Publications by authors named "Meijun Liu"

25 Publications

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Circ-HMGA2 (hsa_circ_0027446) promotes the metastasis and epithelial-mesenchymal transition of lung adenocarcinoma cells through the miR-1236-3p/ZEB1 axis.

Cell Death Dis 2021 Mar 24;12(4):313. Epub 2021 Mar 24.

Medical Oncology Department, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, China.

Lung adenocarcinoma (LUAD) has high incidence and mortality rates worldwide; however, its detailed molecular pathology remains unclear. Although circRNAs have gradually been identified as molecules that are differentially expressed in tumors and play key roles in tumor progression, their role in LUAD is poorly understood. Through microarray analysis, we obtained the circRNA expression profile of LUAD and found that circ-HMGA2 (hsa_circ_0027446), a novel RNA, is highly expressed in LUAD. The high expression of circ-HMGA2 was further verified in 36 paired LUAD and adjacent normal tissues. Functionally, circ-HMGA2 promoted LUAD cell metastasis in vitro and in vivo. The luciferase reporter assay and FISH results showed that circ-HMGA2 interacts with miR-1236-3p and that miR-1236-3p interacts with ZEB1. In addition, miR-1236-3p was expressed at low levels in LUAD, inhibited LUAD cell metastasis, and suppressed the function of circ-HMGA2. ZEB1 is an EMT-promoting transcription factor. The PCR and WB analysis results showed that circ-HMGA2 promotes both ZEB1 expression and EMT. MiR-1236-3p had the opposite effect, reversing the promotive effect of circ-HMGA2 on EMT. In summary, circ-HMGA2 promotes LUAD cell metastasis through the miR-1236-3p/EMT axis, indicating that it could be a therapeutic target in LUAD.
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http://dx.doi.org/10.1038/s41419-021-03601-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991034PMC
March 2021

Analyzing knowledge entities about COVID-19 using entitymetrics.

Scientometrics 2021 Mar 12:1-19. Epub 2021 Mar 12.

Department of Information Management, Peking University, Beijing, China.

COVID-19 cases have surpassed the 109 + million markers, with deaths tallying up to 2.4 million. Tens of thousands of papers regarding COVID-19 have been published along with countless bibliometric analyses done on COVID-19 literature. Despite this, none of the analyses have focused on domain entities occurring in scientific publications. However, analysis of these bio-entities and the relations among them, a strategy called entity metrics, could offer more insights into knowledge usage and diffusion in specific cases. Thus, this paper presents an entitymetric analysis on COVID-19 literature. We construct an entity-entity co-occurrence network and employ network indicators to analyze the extracted entities. We find that ACE-2 and C-reactive protein are two very important genes and that lopinavir and ritonavir are two very important chemicals, regardless of the results from either ranking.
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http://dx.doi.org/10.1007/s11192-021-03933-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953944PMC
March 2021

Luminescent cellulose-based porous binary metal-organic gels in an adsorption bed for effective adsorption and sensitive detection of chlortetracycline hydrochloride.

J Hazard Mater 2021 Feb 25;414:125473. Epub 2021 Feb 25.

Key Lab of Groundwater Resources and Environment (Ministry of Education), Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, 2519 Jiefang Road, Changchun 130021, PR China. Electronic address:

Three novel (Fe-Eu) JLUE-MOGs were successfully fabricated through a solvothermal method and employed to construct the double-effect system for antibiotics adsorption and detection. The characterizations highlighted the properties of ample active sites, large surface areas and hierarchical porous structures, which did contribute to superb and rapid chlortetracycline hydrochloride (CTC) adsorption by JLUE-MOGs. Besides, the effects of initial pH values, JLUE-MOG dosages and co-existing inorganic ions on the CTC adsorption could be explained by pore filling, π-π EDA interaction, electrostatic interaction, water affinity as well as hydrogen bonding. Moreover, the optimized condition was cross-explored by response surface methodology (RSM) with tiny differences compared to actual experiments. In addition, fluorescent JLUE-MOG-7 was implemented for sensitive recognition of CTC and reflecting adsorption processes. Furthermore, shaping JLUE-MOG-7@cellulose aerogels were fabricated as filter materials for applying into an adsorption bed. The breakthrough process was fitted well by Bohart-Adams model and Thomas model, along with recognizable fluorescence changes of immobilized adsorbents. This work develops efficient and luminescent powder-like JLUE-MOGs for antibiotics adsorptive enrichment and sensitive detection. More importantly, immobilized JLUE-MOG@cellulose aerogels, as promising and alternative adsorbents with real-time fluorescence changes, can be utilized for continuously pollutants removal in real wastewater treatment.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125473DOI Listing
February 2021

A prognostic 11-DNA methylation signature for lung squamous cell carcinoma.

J Thorac Dis 2020 May;12(5):2569-2582

Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou 510095, China.

Background: Lung squamous cell carcinoma (LUSC), as the second frequent subtype of lung cancer, causes lots of mortalities primarily due to a lack of precise prognostic markers and timely treatment intervention. Previous studies have constructed several risk prognostic models based on DNA methylation sites in multiple tumors, whereas, DNA methylation signature of LUSC remains to be built, and its predictive value need to be evaluated.

Methods: The genome-wide DNA methylation data of LUSC samples was obtained from The Cancer Genome Atlas dataset. Univariate Cox analysis and the least absolute shrinkage and selection operator (LASSO) were implemented to identify DNA methylation sites related to overall survival of LUSC patients. Thus, we performed multivariate Cox regression to establish a DNA methylation signature. The Kaplan-Meier (K-M) survival curves and time-dependent receiver operating characteristic (ROC) curves were plotted to estimate the prognostic power of the signature. Comparison with other known prognostic biomarkers, our DNA methylation signature showed higher predictive specificity and sensitivity. In addition, multivariate Cox regression screened out independent prognostic factors and constructed a nomogram.

Results: Several statistical methods were performed to construct an 11-DNA methylation signature. LUSC patients were divided into low- and high-risk group based on risk score, and high-risk group had a shorter survival time. According to the results of K-M and ROC analyses, the 11-DNA methylation signature showed significant sensitivity and specificity in predicting the LUSC patients' overall survival. Finally, we integrated some independent prognostic factors (risk score, metastasis stage, and tobacco smoking history) to construct a nomogram, which has excellent prognostic power and may provide guidance for the therapeutic strategies.

Conclusions: We constructed the first risk prognosis model based on DNA methylation site in LUSC, which showed better predictive ability. In addition, a nomogram integrating the DNA methylation signature, metastasis stage, and tobacco smoking history was developed.
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http://dx.doi.org/10.21037/jtd.2020.03.31DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330303PMC
May 2020

Effect of Huatan Jieyu granules in treatment of Parkinson's disease patients with sleep disorder identified as symptom pattern of phlegma-heat-stirring wind.

J Tradit Chin Med 2020 06;40(3):461-466

Medical Department of Neurology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China.

Objective: To investigate the safety and efficacy of Huatan Jieyu granules in treatment of Parkinson's disease (PD) patients with sleep disorder identified as symptom pattern of phlegma-heat-stirring wind in terms of the theory of Traditional Chinese Medicine.

Methods: In total, 107 Parkinson's disease patients with sleep disorders identified as symptom pattern of phlegma-heat-stirring wind were selected and randomly divided into the experimental group (55 cases) and the control group (52 cases). Both groups were given basic treatment with prednisone. The experimental group of patients was treated with Huatan Jieyu granules and the control group of patents was treated with only the basic treatment. Treatment lasted for 4 weeks. Sleep polygraph were recorded before the study as well as 3 months and 6 months after treatment.

Results: After treated with Huatan Jieyu granules, the total sleep time, and the percentage of non rapid eye movement 2 (NREM 2), non rapid eye movement 3 (NREM 3) and rapid eye movement (REM) sleep period increased significantly, while the percentage of NREM1 sleep period decreased significantly compared with before treatment (P < 0.05).

Conclusion: The treatment of PD patients with sleep disorder by Huatan Jieyu granules can improve their sleep structure and their sleep quality.
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http://dx.doi.org/10.19852/j.cnki.jtcm.2020.03.015DOI Listing
June 2020

UrbanMotion: Visual Analysis of Metropolitan-Scale Sparse Trajectories.

IEEE Trans Vis Comput Graph 2020 May 4;PP. Epub 2020 May 4.

Visualizing massive scale human movement in cities plays an important role in solving many of the problems that modern cities face (e.g., traffic optimization, business site configuration). In this work, we study a big mobile location dataset that covers millions of city residents, but is temporally sparse on the trajectory of individual user. Mapping sparse trajectories to illustrate population movement poses several challenges from both analysis and visualization perspectives. In the literature, there are a few techniques designed for sparse trajectory visualization; yet they do not consider trajectories collected from mobile apps that possess long-tailed sparsity with record intervals as long as hours. This work introduces UrbanMotion, a visual analytics system that extends the original wind map design by supporting map-matched local movements, multi-directional population flows, and population distributions. Effective methods are proposed to extract and aggregate population movements from dense parts of the trajectories leveraging their long-tailed sparsity. Both characteristic and anomalous patterns are discovered and visualized. We conducted three case studies, one comparative experiment, and collected expert feedback in the application domains of commuting analysis, event detection, and business site configuration. The result demonstrates significance and effectiveness of our system in completing key analytics tasks for urban users.
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http://dx.doi.org/10.1109/TVCG.2020.2992200DOI Listing
May 2020

A new simple brain segmentation method for extracerebral intracranial tumors.

PLoS One 2020 17;15(4):e0230754. Epub 2020 Apr 17.

School of Clinical Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu University of TCM, Chengdu, China.

Normal brain segmentation is available via FreeSurfer, Vbm, and Ibaspm software. However, these software packages cannot perform segmentation of the brain for patients with brain tumors. As we know, damage from extracerebral tumors to the brain occurs mainly by way of pushing and compressing while leaving the structure of the brain intact. Three-dimensional (3D) imaging, augmented reality (AR), and virtual reality (VR) technology have begun to be applied in clinical practice. The free medical open-source software 3D Slicer allows us to perform 3D simulations on a computer and requires little user interaction. Moreover, 3D Slicer can integrate with the third-party software mentioned above. The relationship between the tumor and surrounding brain tissue can be judged, but accurate brain segmentation cannot be performed using 3D Slicer. In this study, we combine 3D Slicer and FreeSurfer to provide a novel brain segmentation method for extracerebral tumors. This method can help surgeons identify the "real" relationship between the lesion and adjacent brain tissue before surgery and improve preoperative planning.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230754PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164623PMC
July 2020

SRGN crosstalks with YAP to maintain chemoresistance and stemness in breast cancer cells by modulating HDAC2 expression.

Theranostics 2020 4;10(10):4290-4307. Epub 2020 Mar 4.

Affiliated Cancer Hospital & Institute of Guangzhou Medical University; Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation; The State Key Laboratory of Respiratory; Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Hengzhigang Road 78#, Guangzhou 510095, Guangdong, China.

: Chemoresistance is a significant obstacle to the effective treatment of breast cancer (BC), resulting in more aggressive behavior and worse clinical outcome. The molecular mechanisms underlying breast cancer chemoresistance remain unclear. Our microarray analysis had identified the overexpression of a small molecular glycoprotein serglycin (SRGN) in multidrug-resistant BC cells. Here, we aimed to investigate the role of SRGN in chemoresistance of breast cancer and elucidate the underlying mechanisms. : SRNG overexpression was identified using microarray analysis and its clinical relevance was analyzed. To investigate the role of SRGN, we performed various and studies, as well as characterization of serum and tissue samples from BC patients. Chemosensitivity measurement, gene expression interference, immunofluorescence staining, mammosphere assay, flow cytometry analysis, luciferase reporter assay, ChIP-qPCR, coimmunoprecipitation, and immunohistochemistry were performed to explore the potential functions and mechanisms of SRGN. : We confirmed overexpression of SRGN in chemoresistant BC cells and in serum and tissue samples from BC patients with poor response to chemotherapy. SRGN specifically predicted poor prognosis in BC patients receiving chemotherapy. Mechanistically, SRGN promoted chemoresistance both and by cross-talking with the transcriptional coactivator YES-associated protein (YAP) to maintain stemness in BC cells. Ectopic YAP expression restored the effects of knockdown. Inversely, YAP knockdown rescued the effects of overexpression. The secreted SRGN triggered ITGA5/FAK/CREB signaling to enhance transcription. Reciprocally, YAP promoted transcription in a TEAD1-dependent manner to form a feed-forward circuit. Moreover, the YAP/RUNX1 complex promoted transcription to induce chemoresistance and stemness in BC cells. Importantly, the SRGN levels were positively correlated with the YAP and HDAC2 levels in chemoresistant BC tissues. YAP and HDAC2 acted downstream of SRNG and correlated with poor outcomes of BC patients receiving chemotherapy. : Our findings clarify the roles and mechanisms of SRGN in mediating chemoresistance in breast cancer and suggest its use a potential biomarker for chemotherapeutic response. We believe that novel therapeutic strategies for breast cancer can be designed by targeting the signaling mediated by the crosstalk between SRGN and YAP.
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http://dx.doi.org/10.7150/thno.41008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150493PMC
February 2021

[Superficial temporal artery composite perforator flap in repair of temporal skin and soft tissue defects and reconstruction of sideburns].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2020 Apr;34(4):493-496

Department of Burn and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi Guizhou, 563003, P.R.China.

Objective: To investigate the feasibility and effectiveness of repairing temporal skin and soft tissue defects and reconstructing sideburns with superficial temporal artery composite perforator flap.

Methods: Between January 2018 and January 2019, 12 patients with temporal tumors were treated. There were 5 males and 7 females with an average age of 51 years (range, 37-68 years). There were 8 cases of basal cell carcinoma and 4 cases of squamous cell carcinoma. The disease duration ranged from 3 months to 4 years (mean, 13 months). The area of residual wound after tumor resection was 3.8 cm×2.5 cm-5.2 cm×3.5 cm. The superficial temporal artery composite perforator flap was designed. The hairy superficial temporal artery frontal branch perforator flap was used to repair the hair growing area and reconstruct the sideburn; and the area of the flap was 2.5 cm×1.0 cm-4.2 cm×3.0 cm. And the superficial temporal artery descending branch perforator flap without hair was used to repair the hair-free area; and the area of the flap was 2.5 cm×1.5 cm-7.5 cm×4.0 cm. The donor sites were sutured directly.

Results: All flaps survived, and the incisions at the donor and recipient sites healed by first intention. Eleven patients were followed up 6-12 months (mean, 9 months). The incisions were not obvious. The flaps were flat and the color of the flaps were not significantly different from the surrounding skin. The reconstructed sideburns were consistent with the healthy side and the facial appearance was satisfactory. No local tumor recurred during follow-up.

Conclusion: For the temporal skin and soft tissue defects involving the sideburn, the superficial temporal artery composite perforator flap can be used to repair subunits with different aesthetic characteristics in sections and has the advantages of operating simply, obtaining satisfied facial appearance, and little effect on the donor site.
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http://dx.doi.org/10.7507/1002-1892.201909046DOI Listing
April 2020

EEG-Based Brain Functional Connectivity in First-Episode Schizophrenia Patients, Ultra-High-Risk Individuals, and Healthy Controls During P50 Suppression.

Front Hum Neurosci 2019 14;13:379. Epub 2019 Nov 14.

The National Clinical Research Center for Mental Disorders and Beijing Key Laboratory of Mental Disorders, Beijing Anding Hospital, Capital Medical University, Beijing, China.

Dysfunctional processing of auditory sensory gating has generally been found in schizophrenic patients and ultra-high-risk (UHR) individuals. The aim of the study was to investigate the differences of functional interaction between brain regions and performance during the P50 sensory gating in UHR group compared with those in first-episode schizophrenia patients (FESZ) and healthy controls (HC) groups. The study included 128-channel scalp Electroencephalogram (EEG) recordings during the P50 auditory paradigm for 35 unmedicated FESZ, 30 drug-free UHR, and 40 HC. Cortical sources of scalp electrical activity were recomputed using exact low-resolution electromagnetic tomography (eLORETA), and functional brain networks were built at the source level and compared between the groups (FESZ, UHR, HC). A classifier using decision tree was designed for differentiating the three groups, which uses demographic characteristics, MATRICS Consensus Cognitive Battery parameters, behavioral features in P50 paradigm, and the measures of functional brain networks based on graph theory during P50 sensory gating. The results showed that very few brain connectivities were significantly different between FESZ and UHR groups during P50 sensory gating, and that a large number of brain connectivities were significantly different between FESZ and HC groups and between UHR and HC groups. Furthermore, the FESZ group had a stronger connection in the right superior frontal gyrus and right insula than the HC group. And the UHR group had an enhanced connection in the paracentral lobule and the middle temporal gyrus compared with the HC group. Moreover, comparison of classification analysis results showed that brain network metrics during P50 sensory gating can improve the accuracy of the classification for FESZ, UHR and HC groups. Our findings provide insight into the mechanisms of P50 suppression in schizophrenia and could potentially improve the performance of early identification and diagnosis of schizophrenia for the earliest intervention.
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http://dx.doi.org/10.3389/fnhum.2019.00379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6870009PMC
November 2019

Static and continuous flow photoelectrocatalytic treatment of antibiotic wastewater over mesh of TiO nanotubes implanted with g-CN nanosheets.

J Hazard Mater 2020 02 20;384:121248. Epub 2019 Sep 20.

State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, PR China. Electronic address:

Photoelectrocatalytic (PEC) especially continuous flow PEC process for organic wastewater treatment greatly depends on both catalytic capacity and practical availability of electrode materials. In this study, g-CN nanosheets are implanted into TiO nanotube arrays mesh (TCNs) through direct calcination of TiO nanotube array mesh loading melamine precursor. The TCNs photoelectrodes exhibit excellent PEC activity in organic pollutant degradation. Typically, almost 100% of tetracycline (TC, an emerging refractory antibiotic pollutant) is removed in 2 h and TOC removal reaches to 93% in 3 h under simulated solar irradiation at 1 V vs. Ag/AgCl. Theoretical calculations are performed to predict the primary reactive sites for radical species attack and the intermediates are identified. Meanwhile, the ecotoxicity of TC-containing wastewater greatly decrease after PEC treatment. Impressively, because of the mesh screen effect and high catalytic capacity of the photoelectrode, continuous flow PEC process keeps 80% removal efficiency of TC in real wastewater in the absence of additional background electrolyte. After prolonging 20 h, the level of treatment is highly stable. This work would set an example for potential large-scale treatment of organic wastewater using PEC process.
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http://dx.doi.org/10.1016/j.jhazmat.2019.121248DOI Listing
February 2020

Deep Dehalogenation of Florfenicol Using Crystalline CoP Nanosheet Arrays on a Ti Plate via Direct Cathodic Reduction and Atomic H.

Environ Sci Technol 2019 Oct 24;53(20):11932-11940. Epub 2019 Sep 24.

Brook Byers Institute for Sustainable Systems and School of Civil and Environmental Engineering , Georgia Institute of Technology , 828 West Peachtree Street , Atlanta , Georgia 30332 , United States.

Efficient elimination of antibacterial activity of halogenated antibiotics by dehalogenation pretreatment is desired for a biochemical treatment process. In this study, crystalline cobalt phosphide nanosheet arrays on a Ti plate (C-CoP/Ti) are fabricated by a simple electrodeposition and phosphorization process. The crystalline structure greatly promotes atomic hydrogen (H*) generation. Moreover, the nanosheet arrays can provide abundant active sites and accelerate electron transfer and mass transport. As a result, the dehalogenation rate of florfenicol (FLO, an emerging organic pollutant) on C-CoP/Ti is 11.1, 2.97, and 13.6 times higher than that on amorphous CoP/Ti, Pd/Ti, and bare Ti, respectively. The C-CoP/Ti electrode achieves 97.4% dehalogenation of FLO (20 mg L) within 30 min at -1.2 V (vs Ag/AgCl). Nearly 100% of Cl and 20% of F are broken away within 120 min, showing the highest electrocatalytic defluorination efficiency reported so far. Both experimental results and theoretical calculations reveal that the dehalogenation of FLO on C-CoP/Ti is synergistically accomplished via direct reduction of electron transfer and indirect reduction of H*. This study develops a highly efficient non-noble metal electrode material for dehalogenation of halogenated organic compounds.
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http://dx.doi.org/10.1021/acs.est.9b04352DOI Listing
October 2019

Enhanced executive attention efficiency after adaptive force control training: Behavioural and physiological results.

Behav Brain Res 2019 12 18;376:111859. Epub 2019 Mar 18.

Beijing Advanced Innovation Centre for Biomedical Engineering, Beihang University, Beijing, China; School of Mechanical Engineering and Automation, Beihang University, Beijing, China; State Key Lab of Virtual Reality Technology and Systems, Beihang University, Beijing, China. Electronic address:

Attention plays an important role in perception and cognition, and developing an effective method to train and improve attention is an essential and challenging task. In this study, fingertip-based adaptive force control tasks (AFCT) were explored for attention training, and the visual-channel task called an attention network test (ANT) was used to measure the level of attention before and after AFCT. The purposes of this study were to investigate whether AFCT can enhance the attention level on the ANT task and to elucidate the underlying electrophysiological mechanisms. The results showed that the efficiency of the executive control network during ANT was significantly improved by the AFCT training, indicating that the AFCT training may enhance the executive attention level during visual-channel tasks. To measure the behavioural performance during the AFCT training, we used tolerance, variance and duration of the forces to design a comprehensive score of behavioural performance (CSBP), and the electrophysiological mechanisms were also explored using electroencephalography (EEG) recordings. The AFCT and ANT results showed consistency in medial frontal theta activity and in connectivity strength at frontal-parietal regions in the alpha band. These results indicated that the observed attention improvement across tasks executed using different sensory channels may be due to the training of overlapping components of the relevant attention networks. Thus, this study provides further insight into the design of training tasks that stimulate multi-sensory channels, which can be used to improve attention and treat various attention deficit disorders.
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http://dx.doi.org/10.1016/j.bbr.2019.03.028DOI Listing
December 2019

The relation of culture, socio-economics, and friendship to music preferences: A large-scale, cross-country study.

PLoS One 2018 14;13(12):e0208186. Epub 2018 Dec 14.

Institute of Computational Perception, Johannes Kepler University Linz, Linz, Austria.

Music listening is an inherently cultural behavior, which may be shaped by users' backgrounds and contextual characteristics. Due to geographical, socio-economic, linguistic, and cultural factors as well as friendship networks, users in different countries may have different music preferences. Investigating cultural-socio-economic factors that might be associated with between-country differences in music preferences can facilitate music information retrieval, contribute to the prediction of users' music preferences, and improve music recommendation in cross-country contexts. However, previous literature provides limited empirical evidence of the relationships between possible cross-country differences on a wide range of socio-economic aspects and those in music preferences. To bridge this research gap, and drawing on a large-scale dataset, LFM-1b, this study examines the possible relationship between cross-country differences in artist, album, and genre listening frequencies as well as the cross-country distance in geographical, socio-economic, linguistic, cultural, and friendship connections using the Quadratic Assignment Procedure. Results indicate: (1) there is no significant relationship between geographical and economic distance on album, artist, and genre preferences' distance at the country-level; (2) the cross-country distance of three cultural dimensions (masculinity, long-term orientation, and indulgence) is positively associated with both the album and artist preferences distances; (3) the between-country distance in main languages has a positive relationship with the album, artist, and genre preferences distances across countries; (4) the density of friendship connections among countries negatively correlates to the cross-country preference distances in terms of artist and genre. Findings from this study not only expand knowledge of factors related to music preferences at the country level, but also can be integrated into real-world music recommendation systems that consider country-level music preferences.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0208186PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294554PMC
May 2019

Electrocatalytic dechlorination of halogenated antibiotics via synergistic effect of chlorine-cobalt bond and atomic H.

J Hazard Mater 2018 09 3;358:294-301. Epub 2018 Jul 3.

Brook Byers Institute for Sustainable Systems and School of Civil and Environmental Engineering, Georgia Institute of Technology, 828 West Peachtree Street, Atlanta, GA 30332, United States.

Although noble metal electrocatalysts are highly efficient in the dehalogenation of halogenated antibiotics, the prohibitive cost hinders their practical applications. In this study, a cobalt-phosphorous/oxide (CoP/O) composite prepared via a one-step electrodeposition was for the first time applied in electroreductive dechlorination of halogenated antibiotics (HA), including chloramphenicol (CAP), florfenicol (FLO) and thiamphenicol (TAP). CoP/O had a higher FLO dechlorination efficiency (91%) than Pd/C (69.3%) (t = 60 min, C = 20 mg L, applied voltage of -1.2 V vs. saturated calomel electrode (SCE)). Furthermore, the dechlorination efficiencies of CoP/O for CAP and TAP reached to 98.7 and 74.2%, respectively. The electron spin resonance and in situ Raman characterizations confirmed that atomic H* was produced via the CoP and the formation of CoCl bonds occurred on the CoO in CoP/O. The CoCl bond formation could trap HA molecules onto CoP/O and weaken the CCl bond strength. The synergistic effect of H* attack and CoCl bond was responsible for the high dechlorination efficiency. This study offers new insights into the interface mechanism of electroreductive dehalogenation process, and shows a great potential for the remediation of halogenated antibiotics contaminated wastewater.
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http://dx.doi.org/10.1016/j.jhazmat.2018.06.064DOI Listing
September 2018

Compressive Force Spectroscopy: From Living Cells to Single Proteins.

Int J Mol Sci 2018 Mar 23;19(4). Epub 2018 Mar 23.

School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

One of the most successful applications of atomic force microscopy (AFM) in biology involves monitoring the effect of force on single biological molecules, often referred to as force spectroscopy. Such studies generally entail the application of pulling forces of different magnitudes and velocities upon individual molecules to resolve individualistic unfolding/separation pathways and the quantification of the force-dependent rate constants. However, a less recognized variation of this method, the application of compressive force, actually pre-dates many of these "tensile" force spectroscopic studies. Further, beyond being limited to the study of single molecules, these compressive force spectroscopic investigations have spanned samples as large as living cells to smaller, multi-molecular complexes such as viruses down to single protein molecules. Correspondingly, these studies have enabled the detailed characterization of individual cell states, subtle differences between seemingly identical viral structures, as well as the quantification of rate constants of functionally important, structural transitions in single proteins. Here, we briefly review some of the recent achievements that have been obtained with compressive force spectroscopy using AFM and highlight exciting areas of its future development.
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http://dx.doi.org/10.3390/ijms19040960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979447PMC
March 2018

Double-edged sword of interdisciplinary knowledge flow from hard sciences to humanities and social sciences: Evidence from China.

PLoS One 2017 21;12(9):e0184977. Epub 2017 Sep 21.

Department of Information Resource Management, Zhejiang University, Hangzhou, China.

Humanities and Social Sciences (HSS) increasingly absorb knowledge from Hard Sciences, i.e., Science, Technology, Agriculture and Medicine (STAM), as testified by a growing number of citations. However, whether citing more Hard Sciences brings more citations to HSS remains to be investigated. Based on China's HSS articles indexed by the Web of Science during 1998-2014, this paper estimated two-way fixed effects negative binomial models, with journal effects and year effects. Findings include: (1) An inverse U-shaped curve was observed between the percentage of STAM references to the HSS articles and the number of citations they received; (2) STAM contributed increasing knowledge to China's HSS, while Science and Technology knowledge contributed more citations to HSS articles. It is recommended that research policy should be adjusted to encourage HSS researchers to adequately integrate STAM knowledge when conducting interdisciplinary research, as over-cited STAM knowledge may jeopardize the readability of HSS articles.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0184977PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608305PMC
October 2017

MicroRNA-24-1 suppresses mouse hepatoma cell invasion and metastasis via directly targeting O-GlcNAc transferase.

Biomed Pharmacother 2017 Jul 9;91:731-738. Epub 2017 May 9.

School of Life Science and Medicine, Dalian University of Technology, Panjin, China. Electronic address:

MicroRNAs (miRNAs) are endogenous non-coding regulatory RNAs involved in multiple cellular processes. Emerging evidences showed that miRNAs are involved in changing the cell surface glycosylation modification and oncogenesis. In this study, the role of miRNA-24-1 in O-GlcNAcylation and metastasis of mouse hepatocarcinoma cells was investigated. miRNAs expression array profiles were obtained from mouse hepatocarcinoma cell lines Hca-P and Hca-F with the low/high lymphatic metastasis potential, respectively. Based on the miRNAs expression array profiles, miRNA-24-1 expression was found to exhibit converse coincidence with metastasis potential, O-GlcNAc transferase (OGT) expression and O-GlcNAcylation. Dual-luciferase reporter assay revealed that miRNA-24-1 specifically binds to 3'-UTR of OGT. Furthermore, transfecting mouse hepatocarcinoma cells with miR-24-1 mimic and antisense oligonucleotide showed miR-24-mediates OGT expression silencing. This silencing is associated with the suppression of cell metastasis potential, down-regulation of the O-GlcNAcylation on c-Myc and decrease of c-Myc expression at the protein level rather than the mRNA level. Collectively, these results suggested that as a tumor suppressor, miR-24-1 may regulate mouse hepatocarcinoma cells migration and invasion, at least partially through targeting OGT, which could regulate the O-GlcNAcylation and the stability of this oncoprotein c-Myc. This may give insight into a novel mechanism and therapy of tumor lymphatic metastasis.
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http://dx.doi.org/10.1016/j.biopha.2017.05.007DOI Listing
July 2017

Review of systematic reviews and Meta-analyses investigating Traditional Chinese Medicine treatment for type 2 diabetes mellitus.

J Tradit Chin Med 2016 10;36(5):555-63

Objective: To conduct a review of the systematic reviews and meta-analyses on the Traditional Chinese Medicine (TCM) treatment of type 2 diabetes mellitus (T2DM).

Methods: We searched PubMed, Cochrane, Web of Science, Chinese Biomedical Literature Database (CBM), China National Knowledge Infrastructure (CNKI), China Science and Technology Journal Database (VIP), Wanfang, and other databases from database inception to May 2014 for systematic reviews and meta-analyses on TCM treatment of T2DM. Manuscripts were read by two investigators if they met the inclusion criteria, and data were extracted. A Measurement Tool to Assess Systematic Reviews (AMSTAR) was used to classify research quality, and the evidence quality was graded by the Grade of Recommendation, Assessment, Development, and Evaluation (GRADE) system.

Results: Eighteen systematic reviews and meta-analyses were considered. Fifteen analyzed the efficacy of Chinese herbal medicines, and three investigated the efficacy of acupuncture. AMSTAR evaluation ranged from 3-10, and re-evaluation of the main results implied that treatment of T2DM with TCM has certain advantages when compared with conventional Western medicine. However, the evidence quality was generally low.

Conclusion: This work shows favorable evidence for the clinical treatment of TCM on T2DM. However, it is recommended that evidence-based decisions are made based on clinical trials because of the GRADE scores of the studies. To achieve higher quality of clinical research, clinical research on TCM requires specific and suitable research methods. Further trials may increase the quality of evidence to evaluate the clinical efficacy of TCM for T2DM.
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http://dx.doi.org/10.1016/s0254-6272(16)30074-7DOI Listing
October 2016

Mechanisms by which the infection of Sclerotinia sclerotiorum (Lib.) de Bary affects the photosynthetic performance in tobacco leaves.

BMC Plant Biol 2014 Sep 23;14:240. Epub 2014 Sep 23.

Background: Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen which causes disease in a wide range of plants. An observed decrease in photosynthetic performance is the primary reason for the reduction of crop yield induced by S. sclerotiorum. The H2C2O4 is the main pathogenic material secreted by S. sclerotiorum, but the effects of H2C2O4 acidity and the C2O4 2- ion on photosynthetic performance remain unknown.

Results: S. sclerotiorum infection significantly decreased photosynthetic O2 evolution and the maximum quantum yield of photosystem II (Fv/Fm) in tobacco leaves under high-light. H2C2O4 (the main pathogenic material secreted by S. sclerotiorum) with pH 4.0 also significantly decreased photosynthetic performance. However, treatment with H3PO4 and HCl at the same pH as H2C2O4 caused much less decrease in photosynthetic performance than H2C2O4 did. These results verify that the acidity of the H2C2O4 secreted by S. sclerotiorum was only partially responsible for the observed decreases in photosynthesis. Treatment with 40 mM K2C2O4 decreased Fv/Fm by about 70% of the levels observed under 40 mM H2C2O4, which further demonstrates that C2O4(2-) was the primary factor that impaired photosynthetic performance during S. sclerotiorum infection. K2C2O4 treatment did not further decrease photosynthetic performance when D1 protein synthesis was fully inhibited, indicating that C2O4(2-) inhibited PSII by repressing D1 protein synthesis. It was observed that K2C2O4 treatment inhibited the rate of RuBP regeneration and carboxylation efficiency. In the presence of a carbon assimilation inhibitor, K2C2O4 2 treatment did not further decrease photosynthetic performance, which infers that C2O4(2-) inhibited PSII activity partly by repressing the carbon assimilation. In addition, it was showed that C2O4(2-) treatment inhibited the PSII activity but not the PSI activity.

Conclusions: This study demonstrated that the damage to the photosynthetic apparatus induced by S. sclerotiorum is not only caused by the acidity of H2C2O4, but also by C2O4(2-) which plays a much more important role in damaging the photosynthetic apparatus. C2O4(2-) inhibits PSII activity, as well as the rate of RuBP regeneration and carboxylation efficiency, leading to the over production of reactive oxygen species (ROS). By inhibiting the synthesis of D1, ROS may further accelerate PSII photoinhibition.
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http://dx.doi.org/10.1186/s12870-014-0240-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180539PMC
September 2014

Effect of leaf dehydration duration and dehydration degree on PSII photochemical activity of papaya leaves.

Plant Physiol Biochem 2014 Sep 20;82:85-8. Epub 2014 May 20.

College of Life Science, Northeast Forestry University, Harbin 150040, Heilongjiang, China.

Although the effect of dehydration on photosynthetic apparatus has been widely studied, the respective effect of dehydration duration and dehydration degree was neglected. This study showed that, when leaves dehydrated in air, the PSII activities of leaves decreased with the decline of leaf relative water content (RWC). Unexpectedly, when leaves dehydrated to same RWC, the decreases in Fv/Fm, Ψo and RC/CSm were lower in leaves dehydrating at 43 °C than those at 25 °C. However, to reach the same RWC, leaves dehydrating at 43 °C experienced 1/6 of the dehydration duration for leaves dehydrating at 25 °C. To distinguish the respective effect of dehydration degree and dehydration duration on photosynthetic apparatus, we studied the PSII activities of leaves treated with different concentration of PEG solutions. Increasing dehydration degree aggravated the decline of Fv/Fm, Ψo and RC/CSm in leaves with the same dehydration duration, while prolonging the dehydration duration also exacerbated the decline of Fv/Fm, Ψo and RC/CSm in leaves with identical dehydration degree. With the same dehydration degree and duration, high temperature enhanced the decrease of Fv/Fm, Ψo and RC/CSm in the leaves. When leaves dehydrated in air, the effect of high temperature was underestimated due to reduction of dehydration duration. The results demonstrated that, dehydration degree and duration both play important roles in damage to photosynthetic apparatus. We suggest that, under combined stresses, the effects of dehydration degree and duration on plants should be considered comprehensively, otherwise, partial or incorrect results may be obtained.
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http://dx.doi.org/10.1016/j.plaphy.2014.05.003DOI Listing
September 2014

Photoinhibition-like damage to the photosynthetic apparatus in plant leaves induced by submergence treatment in the dark.

PLoS One 2014 19;9(2):e89067. Epub 2014 Feb 19.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shanxi, China.

Submergence is a common type of environmental stress for plants. It hampers survival and decreases crop yield, mainly by inhibiting plant photosynthesis. The inhibition of photosynthesis and photochemical efficiency by submergence is primarily due to leaf senescence and excess excitation energy, caused by signals from hypoxic roots and inhibition of gas exchange, respectively. However, the influence of mere leaf-submergence on the photosynthetic apparatus is currently unknown. Therefore, we studied the photosynthetic apparatus in detached leaves from four plant species under dark-submergence treatment (DST), without influence from roots and light. Results showed that the donor and acceptor sides, the reaction center of photosystem II (PSII) and photosystem I (PSI) in leaves were significantly damaged after 36 h of DST. This is a photoinhibition-like phenomenon similar to the photoinhibition induced by high light, as further indicated by the degradation of PsaA and D1, the core proteins of PSI and PSII. In contrast to previous research, the chlorophyll content remained unchanged and the H2O2 concentration did not increase in the leaves, implying that the damage to the photosynthetic apparatus was not caused by senescence or over-accumulation of reactive oxygen species (ROS). DST-induced damage to the photosynthetic apparatus was aggravated by increasing treatment temperature. This type of damage also occurred in the anaerobic environment (N2) without water, and could be eliminated or restored by supplying air to the water during or after DST. Our results demonstrate that DST-induced damage was caused by the hypoxic environment. The mechanism by which DST induces the photoinhibition-like damage is discussed below.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0089067PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929631PMC
January 2015

A four-domain Kunitz-type proteinase inhibitor from Solen grandis is implicated in immune response.

Fish Shellfish Immunol 2012 Dec 26;33(6):1276-84. Epub 2012 Sep 26.

Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Fisheries Research Institute, Yantai 264006, China.

Serine proteinase inhibitor (SPI) serves as a negative regulator in immune signal pathway by restraining the activities of serine proteinase (SP) and plays an essential role in the innate immunity. In the present study, a Kunitz-type SPI was identified from the mollusk razor clam Solen grandis (designated as SgKunitz). The full-length cDNA of SgKunitz was of 1284 bp, containing an open reading frame (ORF) of 768 bp. The ORF encoded four Kunitz domains, and their amino acids were well conserved when compared with those in other Kunitz-type SPIs, especially the six cysteines involved in forming of three disulfide bridges in each domain. In addition, the tertiary structure of all the four domains adopted a typical model of Kunitz-type SPI family, indicating SgKunitz was a new member of Kunitz-type SPI superfamily. The mRNA transcripts of SgKunitz were detected in all tested tissues of razor clam, including muscle, mantle, gonad, gill, hepatopancreas and hemocytes, and with the highest expression level in gill. When the razor clams were stimulated by LPS, PGN or β-1, 3-glucan, the expression level of SgKunitz mRNA in hemocytes was significantly up-regulated (P < 0.01), suggesting SgKunitz might involved in the processes of inhibiting the activity of SPs during the immune responses triggered by various pathogens. Furthermore, the recombinant protein of SgKunitz could effectively inhibit the activities of SP trypsin and chymotrypsin in vitro. The present results suggested SgKunitz could serve as an inhibitor of SP involving in the immune response of S. grandis, and provided helpful evidences to understand the regulation mechanism of immune signal pathway in mollusk.
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http://dx.doi.org/10.1016/j.fsi.2012.09.015DOI Listing
December 2012

A phase I pharmacokinetics study of 9-nitrocamptothecin in patients with advanced solid tumors.

Cancer Chemother Pharmacol 2011 Apr 30;67(4):955-61. Epub 2010 Dec 30.

School of Chemical Engineering and Technology, Tianjin University, 300072 Tianjin, China.

Purpose: 9-Nitrocamptothecin (9-NC) is a novel orally administered camptothecin analog. The purpose of this study is to evaluate the pharmacokinetics and safety of 9-nitrocamptothecin in patients with advanced solid tumors.

Methods: The 23 patients for a single-dose pharmacokinetic experiment were divided into 3 dosing cohorts. The dosage of 9-nitrocamptothecin capsule was 1.25, 1.5 and 1.75 mg/m(2), respectively. The 8 patients for a multiple-dose pharmacokinetic study were orally administered 9-nitrocamptothecin 1.5 mg/m(2) for 5 consecutive days. Determination of the plasma concentration of 9-nitrocamptothecin was performed by high-performance liquid chromatography-ultraviolet detector technique, and determination of plasma concentration of 9-aminocamptothecin was performed by high-performance liquid chromatography-fluorescence detector technique.

Results: In the single-dose pharmacokinetic study, the mean ± SD 9-nitrocamptothecin C(max) were 94.49 ± 41.38, 115.56 ± 63.27 and 147.57 ± 38.19 ng/mL; AUC(0-36) were 877.14 ± 360.90, 961.33 ± 403.58 and 1,189.75 ± 405.80 ng h/mL, respectively; the mean ± SD 9-aminocamptothecin C(max) were 12.85 ± 6.46, 10.72 ± 6.58 and 28.74 ± 31.94 ng/mL; AUC(0-36) were 157.61 ± 111.61, 88.71 ± 39.51 and 173.52 ± 122.19 ng h/mL, respectively. In the multiple-dose pharmacokinetic study, the mean ± SD 9-nitrocamptothecin AUC(ss) was 907.04 ± 736.47 ng h/mL, C(max) was 85.98 ± 47.52 ng/mL, C(min) was 18.91 ± 22.50 ng/mL, C(av) was 37.79 ± 30.69 ng/mL, DF was 2.16 ± 0.87; the mean ± SD 9-aminocamptothecin AUC(ss) was 442.73 ± 308.39 ng h/mL, C(max) was 34.83 ± 18.31 ng/mL, C(min) was 10.32 ± 6.95 ng/mL, C(av) was 18.45 ± 12.85 ng/mL, DF was 1.34 ± 0.42. Comparing single-dose 1.5 mg/m(2) group with multiple-dose 1.5 mg/m(2) group, no significant difference was observed in 9-NC pharmacokinetic parameters, but with respect to the metabolite, significant differences were observed in C(max) and AUC. The toxicity of 9-NC varied from mild to moderate. No grade 3 or grade 4 toxicity was observed during the study. There was 2- to 13-fold variabilities in 9-NC and 9-AC exposure among different patients for any given dose of 9-NC.

Conclusions: All participants had good tolerance throughout the study. 9-NC and 9-AC exposure did not increase proportionally to the dose ranging from 1.25 to 1.75 mg/m(2). After 5-day continuous administration, accumulation was observed in the metabolite 9-AC, but not in 9-NC.
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http://dx.doi.org/10.1007/s00280-010-1546-zDOI Listing
April 2011