Publications by authors named "Meijing Shi"

5 Publications

  • Page 1 of 1

miR-362-3p Targets Orosomucoid 1 to Promote Cell Proliferation, Restrain Cell Apoptosis and Thereby Mitigate Hypoxia/Reoxygenation-Induced Cardiomyocytes Injury.

Cardiovasc Toxicol 2021 May 18;21(5):387-398. Epub 2021 Jan 18.

Department of Cardiology Center, Hebei General Hospital, No.348 Heping West Road, Shijiazhuang, Hebei, 050051, P.R. China.

This study aimed to investigate the mechanism of how miR-362-3p/orosomucoid 1 (ORM1) involved in hypoxia/reoxygenation (H/R)-induced cardiomyocytes injury. Based on data obtained from Gene Expression Omnibus (GEO) database, we revealed that ORM1 was highly expressed and positively correlated with the expression of inflammatory factors (MAPK1, MAPK3, IL1B and CASP9). miR-362-3p was identified as an upstream regulatory miRNA of ORM1 and negatively modulated the mRNA and protein expression levels of ORM1 in H/R-injured cardiomyocytes. Moreover, we found that miR-362-3p was downregulated in cardiomyocytes injured by H/R. The promoting influence of miR-362-3p mimic on the proliferation and the inhibitory effect of miR-362-3p mimic on the apoptosis of H/R-stimulated cardiomyocytes were eliminated by overexpression of ORM1. Furthermore, miR-362-3p affected the expression of MAPK1, MAPK3, IL1B and CASP9 in H/R-injured cardiomyocytes through targeting ORM1. Our outcomes illustrated that miR-362-3p exhibited a protective influence on H/R-induced cardiomyocytes through targeting ORM1.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12012-020-09631-0DOI Listing
May 2021

Comparative Transcriptomic Analysis of the Development of Sepal Morphology in Tomato ( L.).

Int J Mol Sci 2020 Aug 18;21(16). Epub 2020 Aug 18.

College of Horticulture, Northwest A&F University, Shaanxi 712100, China.

Sepal is an important component of the tomato flower and fruit that typically protects the flower in bud and functions as a support for petals and fruits. Moreover, sepal appearance influences the commercial property of tomato nowadays. However, the phenotype information and development mechanism of the natural variation of sepal morphology in the tomato is still largely unexplored. To study the developmental mechanism and to determine key genes related to downward sepal in the tomato, we compared the transcriptomes of sepals between () mutation and the wild-type by RNA sequencing and found that the differentially expressed genes were dominantly related to cell expansion, auxin, gibberellins and cytokinin. mutation affected cell size and auxin, and gibberellins and cytokinin contents in sepals. The results showed that cell enlargement or abnormal cell expansion in the adaxial part of sepals in . As reported, auxin, gibberellins and cytokinin were important factors for cell expansion. Hence, mutation regulated cell expansion to control sepal morphology, and auxin, gibberellins and cytokinin may mediate this process. One gene and nine genes were dramatically upregulated in the sepal of the mutant, whereas seven genes were significantly downregulated in the sepal of mutant. Further bioinformatic analyses implied that seven / genes might function as negative regulators, while one gene and nine genes might serve as positive regulators of auxin signal transduction, thereby contributing to cell expansion in sepal. Thus, our data suggest that 17 auxin-responsive genes are involved in downward sepal formation in the tomato. This study provides valuable information for dissecting the molecular mechanism of sepal morphology control in the tomato.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms21165914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460612PMC
August 2020

The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells.

Materials (Basel) 2020 Jul 30;13(15). Epub 2020 Jul 30.

National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.

When silk fibroin particles are used for controlled drug delivery, particle size plays a key role in the location of the carrier on the cells as well as the transport pathway, utilization efficiency, and therapeutic effect of the drugs. In this study, the interactions of different-sized silk fibroin particles and cell lines were investigated. Silk fibroin microparticles with dry size of 1.9 ± 0.4 μm (2.7 ± 0.3 μm in wet state) and silk fibroin nanoparticles with dry size of 51.5 ± 11.0 nm (174.8 ± 12.5 nm in wet state) were prepared by salting-out method and high-voltage electrospray method, respectively. CdSe/ZnS quantum dots were coupled to the surface of the micro/nanoparticles. Photostability observations indicated that the fluorescence stability of the quantum dots was much higher than that of fluorescein isothiocyanate. In vitro, microparticles and nanoparticles were co-cultured with human umbilical vein endothelial cells EA.hy 926 and cervical cancer cells HeLa, respectively. The fluorescence test and cell viability showed that the EA.hy926 cells tended to be adhered to the microparticle surfaces and the cell proliferation was significantly promoted, while the nanoparticles were more likely to be internalized in HeLa cells and the cell proliferation was notably inhibited. Our findings might provide useful information concerning effective drug delivery that microparticles may be preferred if the drugs need to be delivered to normal cell surface, while nanoparticles may be preferred if the drugs need to be transmitted in tumor cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma13153372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7436185PMC
July 2020

Transcriptome Profiling of Tomato Uncovers an Involvement of Cytochrome P450s and Peroxidases in Stigma Color Formation.

Front Plant Sci 2017 31;8:897. Epub 2017 May 31.

College of Horticulture, Northwest A&F UniversityYangling, China.

Stigma is a crucial structure of female reproductive organ in plants. Stigma color is usually regarded as an important trait in variety identification in some species, but the molecular mechanism of stigma color formation remains elusive. Here, we characterized a tomato mutant, (), that shows yellow rather than typical green color in the stigma. Analysis of pigment contents revealed that the level of flavonoid naringenin chalcone was increased in the stigma, possibly as a result of higher accumulation of -coumaric acid, suggesting that naringenin chalcone might play a vital role in yellow color control in tomato stigma. To understand the genes and gene networks that regulate tomato stigma color, RNA-sequencing (RNA-Seq) analyses were performed to compare the transcriptomes of stigmas between mutant and wild-type (WT). We obtained 507 differentially expressed genes, in which, 84 and 423 genes were significantly up-regulated and down-regulated in the mutant, respectively. Two cytochrome P450 genes, and which encode -coumarate 3-hydroxylases, and six peroxidase genes were identified to be dramatically inhibited in the yellow stigma. Further bioinformatic and biochemical analyses implied that the repression of the two and six may indirectly lead to higher naringenin chalcone level through inhibiting lignin biosynthesis, thereby contributing to yellow coloration in tomato stigma. Thus, our data suggest that two and six are involved in yellow stigma formation. This study provides valuable information for dissecting the molecular mechanism of stigma color control in tomato. : This study reveals that two cytochrome P450s (SlC3H1 and SlC3H2) and six peroxidases potentially regulate the yellow stigma formation by indirectly enhancing biosynthesis of yellow-colored naringenin chalcone in the stigma of tomato.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2017.00897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449478PMC
May 2017

Gegen Qinlian decoction alleviates experimental colitis via suppressing TLR4/NF-κB signaling and enhancing antioxidant effect.

Phytomedicine 2016 Sep 15;23(10):1012-20. Epub 2016 Jun 15.

State Key Laboratory of Natural Medicines, Department of Biochemistry, China Pharmaceutical University, Nanjing, China, 210009. Electronic address:

Background: Gegen Qinlian decoction (GQ), a Chinese medicinal herb decoction, has been widely used as efficient medicine for the treatment of acute colitis in clinics, but underlying molecular mechanisms have not been fully clarified.

Hypothesis/purpose: Inflammation and oxidative stress have been reported to constitute a crucial part in the pathogenesis of ulcerative colitis (UC). Hence, this study was designed to investigate the antiinflammatory activity and antioxidative effect of GQ.

Study Design: Mice induced by 5% dextran sulfate sodium (DSS) and macrophage RAW264.7 cells stimulated by lipopolysaccharide (LPS) were used in this study.

Methods: Ethanol extracts of GQ were orally administered for 1 week on the dosage of 0.3, 1.5, or 7.5g/kg/day and berberine (BBR, 100mg/kg/d) was selected as a positive group in the animal experiments. In vitro, GQ (25, 50, 100µg/ml) or BBR (20µM) co-cultured with RAW264.7 for 2h prior to LPS stimulation.

Results: The results showed that GQ oral administration alleviated the severity of colitis notably. It reduced toll-like receptor 4 (TLR4) expression and NF-κB activation in mucosa, which was accompanied with down regulation of several inflammatory cytokines in the colon, including tumor necrosis factor (TNF-α), interleukin (IL)-6, IL-1β and IL-4. Furthermore, GQ oral administration attenuated the oxidative stress in the colon of UC mice, evidenced by the decrease of myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, and the elevation of glutathione (GSH) content. In parallel with the vivo experiment results, cell research indicated GQ dramatically reduced the production of TNF-α, IL-6, IL-1β and nitric oxide (NO), as well as that of reactive oxygen species (ROS) upon stimulation of LPS.

Conclusion: Together, our present study indicates that inhibition of TLR4/NF-κB signaling and enhancement of antioxidant effect might be the potential mechanisms for the therapeutic effect of GQ against UC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phymed.2016.06.010DOI Listing
September 2016
-->