Publications by authors named "Mei Yang"

1,054 Publications

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Combination of autophagy and NFE2L2/NRF2 activation as a treatment approach for neuropathic pain.

Autophagy 2021 Apr 9:1-21. Epub 2021 Apr 9.

Department of Anesthesiology, Changzheng Hospital, Naval Medical University, 415 Fengyang Road, Shanghai 200003, China.

Macroautophagy/autophagy, an evolutionarily conserved process, plays an important role in the regulation of immune inflammation and nervous system homeostasis. However, the exact role and mechanism of autophagy in pain is still unclear. Here, we showed that impaired autophagy flux mainly occurred in astrocytes during the maintenance of neuropathic pain. No matter the stage of neuropathic pain induction or maintenance, activation of autophagy relieved the level of pain, whereas inhibition of autophagy aggravated pain. Moreover, the levels of neuroinflammation and reactive oxygen species (ROS) were increased or decreased following autophagy inhibition or activation. Further study showed that inhibition of autophagy slowed the induction, but increased the maintenance of neuroinflammatory responses, which could be achieved by promoting the binding of TRAF6 (TNF receptor-associated factor 6) to K63 ubiquitinated protein, and increasing the levels of p-MAPK8/JNK (mitogen-activated protein kinase 8) and nuclear factor of kappa light polypeptide gene enhancer in B cells (NFKB/NF-κB). Impaired autophagy also reduced the protective effect of astrocytes on neurons against ROS stress because of the decrease in the level of glutathione released by astrocytes, which could be improved by activating the NFE2L2/NRF2 (nuclear factor, erythroid derived 2, like 2) pathway. We also demonstrated that simultaneous activation of autophagy and the NFE2L2 pathway further relieved pain, compared to activating autophagy alone. Our study provides an underlying mechanism by which autophagy participates in the regulation of neuropathic pain, and a combination of autophagy and NFE2L2 activation may be a new treatment approach for neuropathic pain. 3-MA: 3-methyladenine; 8-OHdG: 8-hydroxydeoxy-guanosine; ACTB: actin, beta; AMPAR: alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor; ATG: autophagy-related; CAMK2/CaMKII: calcium/calmodulin-dependent protein kinase II; CCL7: chemokine (C-C motif) ligand 7; CGAS: cyclic GMP-AMP synthase; CQ: chloroquine; GABA: gamma-aminobutyrate; GCLC: glutamate-cysteine ligase, catalytic subunit; GFAP: glial fibrillary acidic protein; GSH: glutathione; HMOX1/HO-1: heme oxygenase 1; KEAP1: kelch-like ECH-associated protein 1; MAP1LC3/LC3-II: microtubule-associated protein 1 light chain 3 beta (phosphatidylethanolamine-conjugated form); MAPK: mitogen-activated protein kinase; MAPK1/ERK: mitogen-activated protein kinase 1; MMP2: matrix metallopeptidase 2; MAPK8/JNK: mitogen-activated protein kinase 8; MAPK14/p38: mitogen-activated protein kinase 14; NFE2L2/NRF2: nuclear factor, erythroid derived 2, like 2; NFKB/NF-κB: nuclear factor of kappa light polypeptide gene enhancer in B cells; ROS: reactive oxygen species; SLC12A5: solute carrier family 12, member 5; SNL: spinal nerve ligation; TLR4: toll-like receptor 4; TRAF6: TNF receptor-associated factor; TRP: transient receptor potential.
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http://dx.doi.org/10.1080/15548627.2021.1900498DOI Listing
April 2021

Fabrication of Cu-hemin metal-organic-frameworks nanoflower supported on three-dimensional reduced graphene oxide for the amperometric detection of HO.

Mikrochim Acta 2021 Apr 8;188(5):160. Epub 2021 Apr 8.

Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400044, People's Republic of China.

A novel electrochemical sensor based on Cu-hemin metal-organic-frameworks nanoflower/three-dimensional reduced graphene oxide (Cu-hemin MOFs/3D-RGO) was constructed to detect HO released from living cells. The nanocomposite was synthesized via a facile co-precipitation method using hemin as the ligand, then decorated with 3D-RGO. The prepared Cu-hemin MOFs showed a 3D hollow spherical flower-like structure with a large specific surface area and mesoporous properties, which could load more biomolecules and greatly enhance the stability by protecting the activity of hemin. In addition, the introduction of 3D-RGO effectively enhanced the conductivity of Cu-hemin MOFs. Thus, the proposed sensor (Cu-hemin MOFs/3D-RGO/GCE) showed excellent electrochemical performances towards HO with a wide linear range (10-24,400 μM), high sensitivity (207.34 μA mM cm), low LOD (0.14 μM), and rapid response time (less than 3 s). Most importantly, we prepared a Cu-hemin MOFs/3D-RGO/ITO electrode with cells growing on it. Compared with detecting HO in cell suspension by GCE-based electrode, adhesion of cells on ITO could shorten the diffusion distance of HO from solution to the surface of the electrode and achieve in situ and a real-time monitor of HO released by living cells. This self-supported sensing electrode showed great potential applications in monitoring the pathological and physiological dynamics of cancer cells.
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http://dx.doi.org/10.1007/s00604-021-04795-0DOI Listing
April 2021

A home-based, post-discharge early intervention program promotes motor development and physical growth in the early preterm infants: a prospective, randomized controlled trial.

BMC Pediatr 2021 Apr 7;21(1):162. Epub 2021 Apr 7.

Department of Neonatology, National Clinical Research Center for Child Health and Disorders; Ministry of Education Key Laboratory of Child Development and Disorders; Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing, China.

Background: The implementation of early intervention (EI) in medical settings is time-consuming and resource-intensive, which limits its extensive use. In 2018, the Chinese Eugenics Association developed a home-based, post-discharge EI program. This study aims at evaluating the impact of this EI program on neurodevelopment and physical growth of early preterm infants.

Methods: This study was a prospective, partially blinded, randomized controlled trial (RCT), followed by an open phase. A total of 73 infants born at 28 ~ 31 weeks' gestation who were admitted to the Children's Hospital of Chongqing Medical University between December 1, 2019, and June 31, 2020, were enrolled. Another 33 infants were retrospectively recruited as the reference group. Thirty-seven infants randomized in the first early intervention, then standard care (EI-SC) group performed a 30-day EI during RCT period, while 36 infants allocated to SC-EI group were given EI in the following open phase. The test of infant motor performance (TIMP), development quotient (DQ), and anthropometric measures (length, weight, head circumference) were measured at the baseline (T0), termination of the RCT (T1), and termination of the open phase (T2). Repeated measures analysis was performed for comparison among groups.

Results: From T0 to T1, both groups had significant improvements in all outcome measures (all p < 0.001). A 30-day EI program was more effective in improving TIMP than standard care (from 53.12 ± 8.79 to 83.50 ± 11.85 in EI-SC group vs from 50.52 ± 8.64 to 75.97 ± 13.44 in SC-EI group, F = 4.232, p = 0.044). EI-SC group also had greater improvements in length, weight, and head circumference than SC-EI group (all p < 0.05). From T0 to T2, there was no significant difference regarding the improvements in all outcomes between the groups (all p > 0.05). At the endpoint of T2, the EI-SC and SC-EI group had similar TIMP and anthropometric measures, but much higher than the reference group (all p < 0.05).

Conclusions: These findings demonstrated that a home-based, post-discharge EI program in this study was a practical approach to promote motor development and physical growth in early preterm infants.

Trial Registration: CHICTR, CTR1900028330, registered December 19, 2019, https:// http://www.chictr.org.cn/showproj.aspx?proj=45706.
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http://dx.doi.org/10.1186/s12887-021-02627-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025314PMC
April 2021

Rechargeable Zn-Air Batteries with Outstanding Cycling Stability Enabled by Ultrafine FeNi Nanoparticles-Encapsulated N-Doped Carbon Nanosheets as a Bifunctional Electrocatalyst.

Nano Lett 2021 Apr 5. Epub 2021 Apr 5.

School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.

Despite grand advances in Zn-air batteries in recently years, their commercialization remains challenging due largely to the lack of efficient bifunctional oxygen catalysts. Herein, we report the crafting of a bifunctional electrocatalyst comprising ultrafine alloyed FeNi nanoparticles encapsulated within N-doped layered carbon nanosheets (denoted FeNi/N-LCN) for high-efficiency Zn-air batteries. The FeNi/N-LCN electrocatalyst is yielded via the coordination of triphenylimidazole-containing polyaniline (TPANI) oligomer with Fe- and Ni-containing precursors, followed by hydrogen binding with melamine and subsequent pyrolysis. The as-constructed FeNi/N-LCN manifests outstanding activity and stability toward both the oxygen reduction reaction (ORR) and oxygen evolution reaction (OER). The primary Zn-air battery assembled with FeNi/N-LCN delivers both high specific capacity and peak power density. Remarkably, the rechargeable Zn-air battery can be repeatedly charged and discharged for 1100 h at 5 mA cm and for 600 h at 10 mA cm, representing the highest cycling stability among various reported Zn-air batteries.
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http://dx.doi.org/10.1021/acs.nanolett.1c00279DOI Listing
April 2021

A hybrid method for heartbeat classification via convolutional neural networks, multilayer perceptrons and focal loss.

PeerJ Comput Sci 2020 30;6:e324. Epub 2020 Nov 30.

School of Electrical Engineering and Automation, Hefei University of Technology, Hefei, Anhui, China.

Background: Heart arrhythmia, as one of the most important cardiovascular diseases (CVDs), has gained wide attention in the past two decades. The article proposes a hybrid method for heartbeat classification via convolutional neural networks, multilayer perceptrons and focal loss.

Methods: In the method, a convolution neural network is used to extract the morphological features. The reason behind this is that the morphological characteristics of patients have inter-patient variations, which makes it difficult to accurately describe using traditional hand-craft ways. Then the extracted morphological features are combined with the RR intervals features and input into the multilayer perceptron for heartbeat classification. The RR intervals features contain the dynamic information of the heartbeat. Furthermore, considering that the heartbeat classes are imbalanced and would lead to the poor performance of minority classes, a focal loss is introduced to resolve the problem in the article.

Results: Tested using the MIT-BIH arrhythmia database, our method achieves an overall positive predictive value of 64.68%, sensitivity of 68.55%, -score of 66.09%, and accuracy of 96.27%. Compared with existing works, our method significantly improves the performance of heartbeat classification.

Conclusions: Our method is simple yet effective, which is potentially used for personal automatic heartbeat classification in remote medical monitoring. The source code is provided on https://github.com/JackAndCole/Deep-Neural-Network-For-Heartbeat-Classification.
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http://dx.doi.org/10.7717/peerj-cs.324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924512PMC
November 2020

Rutin prevents inflammation induced by lipopolysaccharide in RAW 264.7 cells via conquering the TLR4-MyD88-TRAF6-NF-κB signalling pathway.

J Pharm Pharmacol 2021 Mar;73(1):110-117

Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang Liaoning Province, People's Republic of China.

Objectives: Inflammation widely exists in many diseases and poses a great threat to human and animal health. Rutin, quercetin-3-rhamnosyl glucoside, has a variety of pharmacological effects, including anti-oxidant, anti-inflammatory, antibacterial, anticancer and radioresistance effects. The current study focused on evaluation of its anti-inflammatory activity and described the mechanism of rutin in lipopolysaccharide-induced RAW 264.7 cells.

Methods: The related gene and protein expression levels were investigated by quantification real-time PCR and western blotting, respectively.

Key Findings: This study revealed that rutin can decrease inducible nitric oxide synthase (iNOS) gene and protein expression levels, effectively increase IκB gene expression, reduce toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), tumour necrosis factor receptor-associated factor 6 (TRAF6) and p65 gene expression and inhibit the phosphorylation of IκB and p65 and the proteins expression of TLR4, MyD88 and TRAF6.

Conclusions: These results suggest that rutin might exert anti-inflammatory effect on LPS-stimulated RAW 264.7 cells and will be potentially useful as an adjuvant treatment for inflammatory diseases.
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http://dx.doi.org/10.1093/jpp/rgaa015DOI Listing
March 2021

Islet Transplantation Reverses Podocyte Injury in Diabetic Nephropathy or Induced by High Glucose via Inhibiting RhoA/ROCK/NF-B Signaling Pathway.

J Diabetes Res 2021 10;2021:9570405. Epub 2021 Mar 10.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000 Zhejiang Province, China.

Objective: Abnormal signaling pathways play a crucial role in the mechanisms of podocyte injury in diabetic nephropathy. They also affect the recovery of podocytes after islet transplantation (IT). However, the specific signaling abnormalities that affect the therapeutic effect of IT on podocytes remains unclear. The purpose of this study was to assess whether the RhoA/ROCK/NF-B signaling pathway is related to podocyte restoration after IT.

Methods: A mouse model of diabetic nephropathy was established using streptozotocin. The mice were then subsequently reared for 4 weeks after islet transplantation to determine the effect of IT. Islet cells, CCG-1423 (RhoA Inhibitor), and fasudil (ROCK inhibitor) were then cocultured with podocytes to assess their protective effects on podocyte injury induced by high glucose (HG). Protein expression levels of RhoA, ROCK1, synaptopodin, IL-6, and MCP-1 in kidney tissues were then measured using immunohistochemistry and Western blotting techniques.

Results: Islet transplantation reduced the expression levels of RhoA/ROCK1 and that of related inflammatory factors such as IL-6 and MCP-1 in the kidney podocytes of diabetic nephropathy. In the same line, islet cells reduced the expression of RhoA, ROCK1, and pp65 in immortalized podocytes under high glucose (35.0 mmol/L glucose) conditions.

Conclusions: Islet transplantation can reverse podocyte injury in diabetes nephropathy by inhibiting the RhoA/ROCK1 signaling pathway. Islet cells have a strong protective effect on podocytes treated with high glucose (35.0 mmol/L glucose). Discovery of signaling pathways affecting podocyte recovery is helpful for individualized efficacy evaluation and targeted therapy of islet transplantation patients.
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http://dx.doi.org/10.1155/2021/9570405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969114PMC
March 2021

A novel multifunctional platform based on ITO/APTES/ErGO/AuNPs for long-term cell culture and real-time biomolecule monitoring.

Talanta 2021 Jun 1;228:122232. Epub 2021 Mar 1.

Key Laboratory for Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, PR China; Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, 400044, PR China. Electronic address:

Integrating long-term cell culture with real-time electrochemical monitoring is a promising strategy for future studies of physiological and pathological processes. However, great challenges still remain in fabricating such a platform with satisfactory electrochemical performance as well as desirable biocompatibility. Herein, we proposed a novel multifunctional platform based on gold nanoparticles/electrochemically reduced graphene oxide/3-aminopropyl-triethoxysilane modified indium tin oxide plate (ITO/APTES/ErGO/AuNPs). The unique biological and electrical properties of AuNPs and ErGO endow the platform with superior electrocatalytic activity and desirable biocompatibility. As a proof of concept, the present platform showed satisfactory electrochemical performance for sensitive and selective detection of hydrogen peroxide (HO) with a sensitivity about 0.25 μA μM cm and a detection limit of 0.38 μM in a linear range of 0.5-1461 μM. And the principle of catalytic reduction was clarified through density functional calculations (DFT). Furthermore, cells grew on the platform exhibited excellent proliferation ability and considerable viability after a long-term cultivation. Based on those desirable performances, in-situ and real-time monitoring of endogenously produced HO released from cancer cells cultured on the platform has been successfully realized, which will be of great significance in pathophysiology research.
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http://dx.doi.org/10.1016/j.talanta.2021.122232DOI Listing
June 2021

PBK/TOPK promotes chemoresistance to oxaliplatin in hepatocellular carcinoma cells by regulating PTEN.

Acta Biochim Biophys Sin (Shanghai) 2021 Mar 27. Epub 2021 Mar 27.

Department of Clinical Laboratory, SSL Central Hospital of Dongguan City, The Third People's Hospital of Dongguan City, Dongguan 523326, China.

Oxaliplatin (OXA) resistance limits the efficiency of treatment for hepatocellular carcinoma (HCC). Studies have shown that the PDZ-binding kinase (PBK) plays important roles in tumors. However, the role of PBK in HCC is still a problem. In this study, we explored whether PBK is involved in the chemoresistance to OXA in HCC. Expressions of PBK in six HCC cell lines and one human hepatocytes line were determined by real-time quantitative PCR and western blot analysis. SNU-182 and HepG2 cells were chosen to induce OXA resistance. PBK was silenced or overexpressed in OXA-resistant and sensitive cell lines. Then, cell proliferation, migration, and invasion were measured by cholecystokinin-8 assay and Transwell assay, respectively. The Cancer Genome Atlas dataset showed that PBK is highly expressed in HCC and signifies poor prognosis to patient with HCC. Results showed that expression of PBK in HCC cells was significantly higher than that in THLE2 cells, and it was further increased in OXA-resistant HCC cells. Silencing of PBK promoted the sensitivity of drug-resistant HCC cells to OXA. Overexpression of PBK relieved the apoptosis induced by OXA and promoted the migration and invasion of OXA-sensitive HCC cells. Thus, this study revealed that high PBK expression is correlated with OXA resistance in HCC cells, which may provide a promising therapeutic target for treating HCC.
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http://dx.doi.org/10.1093/abbs/gmab028DOI Listing
March 2021

Effect of gut microbiota on in vitro bioaccessibility of heavy metals and human health risk assessment from ingestion of contaminated soils.

Environ Pollut 2021 Mar 15;279:116943. Epub 2021 Mar 15.

College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, PR China; Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing, 100085, PR China. Electronic address:

To identify the role of gut microbiota in human health risk assessment, the bioaccessibility of heavy metals in 14 soil samples were determined in simulated gastrointestinal fluids. Compared to the small intestinal phase, the bioaccessibility values of the colon phase varied, either increased by 3.5-fold for As, by 2.2-fold for Cr, and by 1.6-fold for Ni, or reduced by 4.4-fold for Cu, respectively. The colon incubation with adult gut microbiota yielded higher bioaccessibility value of As (1.3 times) and Fe (3.4 times) than that of the child in most soil samples. Colon bioaccessibility was about 60% greater of Cd for the adult and 30% higher of Cr for the child. Congruent data on the bioaccessibility of Cu and Ni was observed. In addition, correlation analysis indicated that in vitro bioaccessibility was primarily related to total concentrations of heavy metals in soils, followed by soil pH and active Fe/Mn oxide. Significantly, risk assessment calculated based on colon bioaccessibility indicated that the target hazard quotient (THQ > 1) of As was presented in 3 soil samples for the adult (1.05-3.35) and in 9 soil samples for the child (1.06-26.93). The hazard index (HI) of the child was 4.00 on average, greater than that of the adult (0.62), primarily due to the contribution of As and Cd. It suggested non-carcinogenic risks are likely to occur in children through typical hand-to-mouth behavior. The adjustment of colon bioaccessibility will result in more accurate risk assessment of human exposure to heavy metals from oral ingestion of contaminated soils.
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http://dx.doi.org/10.1016/j.envpol.2021.116943DOI Listing
March 2021

MiR-520a-3p Inhibited Macrophage Polarization and Promoted the Development of Atherosclerosis via Targeting UVRAG in Apolipoprotein E Knockout Mice.

Front Mol Biosci 2020 9;7:621324. Epub 2021 Mar 9.

Department of Geratology, Cangzhou Central Hospital, Cangzhou, China.

Atherosclerosis (AS), a kind of chronic inflammatory blood vessel disease, is a main cause of cardiovascular disease, which is a leading cause of mortality around the world. Accumulation of macrophages induced by inflammation contributes to AS development. It has been indicated that microRNAs (miRNAs) are involved in the process of AS. However, the pathway and gene miRNAs targeting are poorly understood. Here we reported that miR-520a-3p was increased in mice with AS and silencing of miR-520a-3p attenuated AS process. Furthermore, inhibition of miR-520a-3p increased the expression of α-SMA and collagen. In addition, miR-520a-3p silencing inhibited the expression of M1 macrophage polarization markers and pro-inflammatory genes and promoted the M2 macrophage polarization. What's more, forced expression of miR-520a-3p diminished IL4/IL13 induced macrophage autophagy via targeting UVRAG. Collectively, our study reveals the role of miR-520a-3p in macrophage polarization and suggests the potential of miRNA as a novel treatment target of AS.
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http://dx.doi.org/10.3389/fmolb.2020.621324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985160PMC
March 2021

Development of an X-Band Reflectarray Antenna for Satellite Communications.

Sci Rep 2021 Mar 22;11(1):6530. Epub 2021 Mar 22.

Beijing Institute of Spacecraft System Engineering, Beijing, 100094, China.

An X-band reflectarray antenna using 16 × 12 double square ring elements for satellite communications is proposed in this paper. The feed is a 4 × 3 elements microstrip patch array designed to create edge taper of approximately - 10 dB. A prototype with right-hand circular polarization (RHCP) is manufactured and tested, and the good agreements between simulations and measurements are demonstrated. The good performance is obtained with the aperture efficiency of 40.7% and the 3-dB gain bandwidth of about 10% which is beneficial to nanosatellites.
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http://dx.doi.org/10.1038/s41598-021-85132-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985410PMC
March 2021

Molecular Diversity and Distribution of Arbuscular Mycorrhizal Fungi at Different Elevations in Mt. Taibai of Qinling Mountain.

Front Microbiol 2021 4;12:609386. Epub 2021 Mar 4.

College of Agriculture, Henan University of Science and Technology, Luoyang, China.

Arbuscular mycorrhizal fungi (AMFs) play a vital role in ecosystems, especially in ecosystem variability, diversity, and function. Understanding the AMF diversity, distribution, and their driver at different altitudinal gradients is a benefit for understanding the ecological function of AMF in mountain ecosystems. In this study, we explored the AMF molecular diversity and their distribution from 660 to 3,500 m a.s.l. in Mount Taibai of Qinling Mountains based on high-throughput sequencing technology. A total of 702 operational taxonomic units (OTUs) in 103 species of AMF are isolated from soil samples, which belong to 18 identified and 1 unidentified genus in 10 families. The fungi in the genus of is the most dominant, with the occurrence frequency of 100% and the relative abundance of 42.268% and 33.048% on the species and OTU level, respectively. The AMF colonization in root could be simulated by a cubic function with the change of altitudes with the peak and trough at a.s.l. 1,170 and 2,850 m, respectively. Further, AMF diversity indices including Sob, Shannon diversity, and Pielou evenness also showed the same cubic function change trends with increasing altitude at OTU and species levels. However, the average values of diversity indices at OTU level are always higher than these at the species level. Based on the OTU level, the highest and lowest values of Shannon and Pielou indices are observed at the altitudes of 1,400 and 2,800 m, respectively. The pattern of AMF community distribution in Mt. Taibai is driven by altitude with the characteristics of more abundance in the medium- to low-altitude than high-altitude areas. In general, abundant AMF molecular diversity and species exit in different elevations of Mt. Taibai, which indicate gradient changes with elevations.
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http://dx.doi.org/10.3389/fmicb.2021.609386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974767PMC
March 2021

S100A4 in Spinal Substantia Gelatinosa from Dorsal Root Ganglia Modulates Neuropathic Pain in a Rodent Spinal Nerve Injury Model.

J Pain Res 2021 10;14:665-679. Epub 2021 Mar 10.

Department of Neurobiology, Key Laboratory of Molecular Neurobiology, Ministry of Education, Naval Medical University, Shanghai, People's Republic of China.

Purpose: To detect the spatio-temporal expression of S100A4 in a spinal nerve ligation (SNL) rat model. Also to figure out which other molecules directly interact with S100A4 to explore the possible mechanisms which might be involved in neuropathic pain.

Methods: Seven-week-old male SD rats were used for the SNL model construction. Immunofluorescence and Western blotting were used to detect the spatio-temporal expression of S100A4 in the model. S100A4 was co-labeled with a number of related molecules and marker molecules that can distinguish between cell types. After intrathecal injection of S100A4 neutralizing antibody, the behavioral changes of SNL rats were recorded, and molecular changes compared. The direct interaction between S100A4 and other related molecules was verified by co-immunoprecipitation (co-IP) to explore its possible mechanism.

Results: After spinal nerve ligation, the content of S100A4 in the dorsal root ganglion (DRG) and spinal dorsal horn increased significantly. Intrathecal injection of S100A4 neutralizing antibody could effectively relieve the mechanical pain in rats. co-IP revealed a direct interaction between S100A4 and RAGE.

Conclusion: The content of S100A4 in the DRG and spinal dorsal horn of SNL rats increased, compared with that of the control group. Intrathecal injection of S100A4 neutralizing antibody could effectively relieve the mechanical pain in SNL rats. S100A4 may be involved in the production of neuropathic pain through RAGE or other ways, but the specific mechanism needs to be further studied.
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http://dx.doi.org/10.2147/JPR.S293462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956897PMC
March 2021

Development of an indirect ELISA for detection of anti-Mycoplasma hyopneumoniae IgG in naturally infected pathogen-induced convalescent sera.

BMC Vet Res 2021 Mar 16;17(1):123. Epub 2021 Mar 16.

Laboratory of Veterinary Mycoplasmology, College of Veterinary Medicine, Southwest University, 2 Tiansheng Road, Beibei District, 400715, Chongqing, China.

Background: Immunization of pigs with an inactivated Mycoplasma hyopneumoniae vaccine (bacterin) generates hyperimmune serum that contains high concentrations of anti-M. hyopneumoniae IgG. Commercially available IgG-ELISA kits cannot distinguish between anti-M. hyopneumoniae IgG in inactivated bacterin-induced hyperimmune sera and convalescent sera resulting from natural M. hyopneumoniae infection. Establishment of an ELISA to detect anti-M. hyopneumoniae IgG in convalescent sera will facilitate the evaluation of the M. hyopneumoniae status of pig farms.

Results: In this study, we expressed and purified recombinant Mhp366-N protein, which contains an epitope recognized by M. hyopneumoniae convalescent sera but not hyperimmune sera, for use as a coating antigen. For the M. hyopneumoniae convalescent serum IgG-ELISA, the optimal antigen concentration, blocking buffer, blocking time, dilution of serum, incubation time with serum, secondary antibody dilution, secondary antibody incubation time and colorimetric reaction time were 0.25 µg/mL, 2.5 % skim milk, 1 h, 1:500, 0.5 h, 1:10,000, 1 h and 15 min, respectively. Validation of the M. hyopneumoniae convalescent serum IgG-ELISA showed a cut-off value of 0.323, the intra-assay CV ranged from 3.27 to 7.26 %, the inter-assay CV ranged from 3.46 to 5.93 %, and the assay was able to differentiate convalescent sera from antibodies to 7 other porcine respiratory pathogens. The convalescent serum IgG-ELISA detected no anti-M. hyopneumoniae IgG in hyperimmune serum samples while a commercial IgG-ELISA identified 95/145 of these sera as positive. The accuracy of the M. hyopneumoniae convalescent serum IgG-ELISA was comparable to the sIgA-ELISA but better than the commercial IgG-ELISA.

Conclusions: The convalescent serum IgG-ELISA is a reproducible, sensitive, and specific indirect ELISA to detect anti-M. hyopneumoniae IgG in naturally infected pathogen-induced convalescent sera. This ELISA could be used to carry out large scale surveillance of M. hyopneumoniae infection in pig farms regardless of vaccination status.
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http://dx.doi.org/10.1186/s12917-021-02828-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968261PMC
March 2021

Electrically injected GaN-based microdisk towards an efficient whispering gallery mode laser.

Opt Express 2021 Feb;29(4):5598-5606

III-nitrides based microdisks with the mushroom-type shape are key components for integrated nanophotonic circuits. The air gap undercut in the mushroom-type microdisk is essential for maintaining vertical optical confinement, but this structure is still facing the difficulty of electrical injection. In this work, we demonstrate an electrically injected GaN-based microdisk of such structure. The device is featured with a copper substrate and copper supporting pedestal, through which current can be efficiently injected into the microdisk with low leakage current (less than 10 nA). Bright emission at ∼420 nm was demonstrated from the microdisk under current injection. The copper substrate and supporting pedestal can also extract thermal energy out of the microdisk effectively, and the structure in this work shows a low thermal resistance of ∼788.86 K/W. Low threshold lasing action at ∼405 nm was realized under the optically pumped condition and the threshold energy is ∼35 nJ/pulse. Clear whispering gallery modes were observed and the Q factor is as high as 4504, indicating the high quality of the microdisk cavity. This work is the first step towards low threshold efficient electrically injected microdisk laser with a mushroom-type shape.
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http://dx.doi.org/10.1364/OE.416873DOI Listing
February 2021

Hemorrhagic Shock, Aorto-Esophageal Fistula, and Thoracic Aorta Pseudoaneurysm Caused by Fish Bone.

Circ Cardiovasc Imaging 2021 Mar 12;14(3):e011476. Epub 2021 Mar 12.

The Cardiac Surgery Department (G.C., M.L., Z.W.), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

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http://dx.doi.org/10.1161/CIRCIMAGING.120.011476DOI Listing
March 2021

Resource utilization and costs among patients with heart failure with reduced ejection fraction following a worsening heart failure event.

ESC Heart Fail 2021 Mar 10. Epub 2021 Mar 10.

Department of Medicine, University of Mississippi Medical Center, Jackson, MS, USA.

Aims: The aim of this study is to characterize healthcare resource utilization and costs in patients with heart failure with reduced ejection fraction (HFrEF) following a worsening heart failure event.

Methods And Results: This was a retrospective observational cohort analysis. Patients with HFrEF were identified from the PINNACLE Registry and linked to a nationwide pharmacy and medical claims database. Worsening heart failure was defined as stable heart failure with a subsequent hospitalization and/or intravenous diuretic therapy. Healthcare resource use and costs in 2015 US dollars were analysed for dispensed prescriptions, outpatient encounters, and hospital encounters. Among 11 064 patients with HFrEF, 3087 (27.9%) experienced a worsening heart failure event during an average follow-up of 973 days. During the first 30 days after the worsening event, 19.8% of patients had hospital readmissions with heart failure as the primary or secondary diagnosis. During that same time period, mean per patient heart failure-related healthcare resource use included 1.3 prescriptions, 0.5 practitioner visits, and 0.5 hospital encounters (admissions, observations, or emergency care), for an average total medical cost of $8779 per patient including $5359 in heart failure-related costs. During the first year following worsening heart failure onset, mean per patient total and heart failure-related costs were $62 615 and $35 329, respectively.

Conclusions: The economic burden following a worsening heart failure event calls for further review of methods to prevent progressive disease, improve adherence to guideline-directed therapy, and develop novel treatments and care strategies to moderate further progression.
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http://dx.doi.org/10.1002/ehf2.13155DOI Listing
March 2021

Feature-Level Attention-Guided Multitask CNN for Fault Diagnosis and Working Conditions Identification of Rolling Bearing.

IEEE Trans Neural Netw Learn Syst 2021 Mar 8;PP. Epub 2021 Mar 8.

Accurate and real-time fault diagnosis (FD) and working conditions identification (WCI) are the key to ensuring the safe operation of mechanical systems. We observe that there is a close correlation between the fault condition and the working condition in the vibration signal. Most of the intelligent FD methods only learn some features from the vibration signals and then use them to identify fault categories. They ignore the impact of working conditions on the bearing system, and such a single-task learning method cannot learn the complementary information contained in multiple related tasks. Therefore, this article is devoted to mining richer and complementary globally shared features from vibration signals to complete the FD and WCI of rolling bearings at the same time. To this end, we propose a novel multitask attention convolutional neural network (MTA-CNN) that can automatically give feature-level attention to specific tasks. The MTA-CNN consists of a global feature shared network (GFS-network) for learning globally shared features and K task-specific networks with feature-level attention module (FLA-module). This architecture allows the FLA-module to automatically learn the features of specific tasks from globally shared features, thereby sharing information among different tasks. We evaluated our method on the wheelset bearing data set and motor bearing data set. The results show that our method has a better performance than the state-of-the-art deep learning methods and strongly prove that our multitask learning mechanism can improve the results of each task.
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http://dx.doi.org/10.1109/TNNLS.2021.3060494DOI Listing
March 2021

Lipoprotein glomerulopathy induced by ApoE Kyoto mutation in ApoE-deficient mice.

J Transl Med 2021 03 4;19(1):97. Epub 2021 Mar 4.

Department of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Sichuan, Guoxue Alley, 37#, Wuhou District, Chengdu, 610041, China.

Background: Lipoprotein glomerulopathy (LPG) is a rare autosomal dominant kidney disease that is most commonly caused by mutations in ApoE Kyoto (p.R43C) and ApoE Sendai (p.R163P). Differences in phenotype among the various ApoE mutations have been suggested, but the pathogenic role of ApoE Kyoto has not been validated in an animal model. This study intended to establish an ApoE Kyoto murine model and to further compare the pathologic differences between ApoE Kyoto and ApoE Sendai.

Method: Male ApoE-deficient mice, 3 months of age, were divided into five groups, including the AD-ApoE Sendai, AD-ApoE Kyoto, AD-ApoE3, AD-eGFP, and ApoE (-/-) groups. The first four groups received recombinant adenovirus that contained the entire coding regions of the human ApoE Sendai and ApoE Kyoto, apoE3, and eGFP genes, respectively. Fasting blood and urine samples were collected at multiple time points. Lipid profiles and urine albumin-creatinine ratio were measured. Renal and aortic histopathologic alterations were analyzed.

Results: After virus injection, plasma human ApoE was detected and rapidly reached the maximum level at 4-6 days in the AD-ApoE Kyoto and AD-ApoE Sendai groups (17.4 ± 3.1 µg/mL vs.: 22.2 ± 4.5 µg/mL, respectively) and at 2 days in the AD-ApoE3 group (38.4 µg/mL). The serum total cholesterol decreased by 63%, 65%, and 73% in the AD-ApoE Kyoto, AD-ApoE Sendai and AD-ApoE3 groups, respectively. There were no significant changes in serum triglyceride and urinary albumin-creatinine ratio among the five groups. Typical lipoprotein thrombi with positive ApoE staining were detected in the AD-ApoE Kyoto and AD-ApoE Sendai groups. The Oil-red O-positive glomerular area tended to be higher in the AD-ApoE Kyoto group (9.2%) than in the AD-ApoE Sendai (3.9%), AD-ApoE3 (4.8%), AD-eGFP (2.9%), and ApoE (-/-) (3.6%) groups. The atherosclerotic plaque area in the aorta was lower in the group injected with various ApoE mutations than in the group without injection of ApoE mutation.

Conclusions: In this animal study, we first established an ApoE Kyoto mutation murine model and confirmed its pathogenic role in LPG. Our results suggested that LPG may be more severe with the ApoE Kyoto than with the ApoE Sendai.
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http://dx.doi.org/10.1186/s12967-021-02765-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934380PMC
March 2021

Treatment of exostosin 1-associated membranous lupus nephritis with multiple low doses of rituximab: A case report.

Medicine (Baltimore) 2021 Mar;100(9):e24887

Renal Division, Department of Medicine.

Rationale: Membranous glomerulonephritis (MN) is the leading cause of nephrotic syndrome in adults and is classified as primary or secondary. Secondary MN accounts for 20% to 30% of all MN cases and can arise from a number of conditions, including autoimmune diseases. Recently exostosin 1/exostosin 2 (EXT1/EXT2) have been identified as the common antigens in secondary autoimmune MN and are present in cases of pure membranous lupus nephritis (LN). The treatment of EXT1/EXT2-associated MN remains elusive.

Patient Concerns: We present the case of a 15-year-old female who presented with nephrotic syndrome, positive ANA and dsDNA, and low serum complements. A renal biopsy revealed pure membranous nephritis with IgG and C3 deposition. EXT1 was found along the glomerular capillary walls and stained positive, while phospholipase A2 receptor (PLA2R) and thrombospondin type-1 domain-containing 7A (THSD7A) were negative.

Diagnosis: The patient was diagnosed with ETX1-associated membranous LN.

Interventions: She was treated with prednisone and multiple low-dose rituximab (4 200 mg doses, approximately every 2 months, based on CD19+ cells counts).

Outcomes: The patient had complete remission within 8 months later, and she remained in remission for the 16-month period of follow-up.

Lessons: To our knowledge, this is the first case of EXT1-associated MN that has been successfully treated by multiple low-dose rituximab. Further studies can investigate the optimal dosage and treatment protocol.
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http://dx.doi.org/10.1097/MD.0000000000024887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939150PMC
March 2021

Association between ambient air pollution and mortality from chronic obstructive pulmonary disease in Wuhan, China: a population-based time-series study.

Environ Sci Pollut Res Int 2021 Mar 1. Epub 2021 Mar 1.

Wuhan Centers for Disease Control and Prevention, No.288 Machang Road, Wuhan, China.

Evidence on the short-term effects of ambient air pollution on chronic obstructive pulmonary disease (COPD) mortality is still not conclusive. The aim of this study was to investigate the relationships between them in Wuhan China. Daily death numbers, concentrations of air pollutants (PM, PM, SO, NO, and O), and meteorological characteristics in Wuhan from January 1, 2014, to December 31, 2019, were collected. Time-series analysis using generalized additive model was applied. The results showed that a total of 16,150 deaths (7.37 deaths per day) from COPD were observed. The daily average concentrations of PM, PM, SO, NO, and O were 59.03, 90.48, 12.91, 48.84, and 91.77 μg/m, respectively. In single pollutant model, for every increase of 10 μg/m in PM, SO, and NO levels, COPD mortality increased by 0.583% (95% CI: 0.055-1.113%), 4.299% (95% CI: 0.978-7.729%), and 1.816% (95% CI: 0.515-3.313%) at lag03, respectively. No significant associations were found for PM and O. Subgroup analysis demonstrated that females were more susceptible to PM, PM, SO, and NO. The concentrations of PM, SO, and NO were significantly associated with COPD mortality for older adults. The effects of PM and O on COPD mortality were higher in warm period. In two-pollutant models, the significantly positive associations between SO and NO and COPD mortality remained after adjusting for PM or O. In conclusions, short-term exposure to PM, SO, and NO are significantly associated with a higher risk of COPD mortality. Female or elderly are more susceptible to air pollution. It is urgent to implement the environmental protection policy.
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http://dx.doi.org/10.1007/s11356-021-13180-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920403PMC
March 2021

[Contrastive analysis of extraction of Polygonatum cyrtonema planting area based on data of "Resource 3"].

Zhongguo Zhong Yao Za Zhi 2021 Jan;46(2):267-271

State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

Polygonatum cyrtonema is a famous bulk medicinal material which is the medicinal and edible homologous. With the implementation of the traditional Chinese medicine industry to promote precise poverty alleviation, the planting area of P. cyrtonema in Jinzhai is becoming larger and larger in recent years. Jinzhai is located in the Dabie Mountainous area, which is the largest mountain area and county in Anhui Province. The cultivation of P. cyrtonema is scattered, and the traditional Chinese medicine resources investigation is not only inefficient and accurate. In this study,the "Resource 3"(ZY-3) remote sensing image was used as the best observation phase,and the method of support vector machine classification was used. The method of parallelepiped, minimum distance, mahalanob is distance, maximum likelihood classification and neural net were used to classify and recognize the P. cyrtonema in the whole region. In order to determine the accuracy and reliability of classification results, the accuracy of six supervised classification results was evaluated by confusion matrix method, and the advantages and disadvantages of six supervised classification methods for extracting P. cyrtonema field planting area were compared and analyzed. The results showed that the method of support vector machine classification was more appropriate than that using other classification methods. It provides a scientific basis for monitoring the planting area of P. cyrtonemain field.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20201026.102DOI Listing
January 2021

[Suitable planting area of Poria cocos in Jinzhai county of Dabie Mountains region].

Zhongguo Zhong Yao Za Zhi 2021 Jan;46(2):260-266

State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

Dabie Mountain in Anhui province is a genuine producing area of Poria cocos, commonly known as Anling. Jinzhai county in Anhui province is a traditional producing area of P. cocos, and it is also a key county for poverty alleviation in Dabie Mountains. Poverty alleviation of traditional Chinese medicine producing area is an important measure to implement the major strategic deployment of the central government. The planting of P. cocos is helpful to promote the development of traditional Chinese medicine industry in Dabie Mountains and help poverty alleviation. P. cocos is a saprophytic fungus with special demands on soil and ecological environment, and its planting appears a scattered and irregular distribution. Traditional investigation methods are time-consuming and laborious, and the results are greatly influenced by subjective factors. In order to obtain the suitable planting area of P. cocos in Jinzhai county, according to the field survey, the research team has explored the regional, biological characteristics and cultivation methods of P. cocos in the county, and obtained the altitude distribution area suitable for the growth of P. cocos. Then, the MaxEnt niche model was used to analyze the relationship between ecological factors and distribution areas, and the potential distribution zoning of P. cocos in Jinzhai county was studied. Combined with the characteristics of P. cocos planting pattern, taking ZY-3 remote sensing image as the data source, the maximum likelihood method was used to extract the area that could be used for P. cocos cultivation in Jinzhai county, and the reason why artificial planting P. cocos was mainly distributed in the west of Jinzhai county was analyzed. The suitable regional classification of P. cocos in Jinzhai county was obtained by superposition of suitable altitude distribution area, MaxEnt analysis and area extracted from remote sensing image, which provided data support for the planting planning of P. cocos in Jinzhai county.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20201024.102DOI Listing
January 2021

Oxygen content-related DNA damage of graphene oxide on human retinal pigment epithelium cells.

J Mater Sci Mater Med 2021 Feb 27;32(2):20. Epub 2021 Feb 27.

State Key Laboratory of Ophthalmology, Optometry and Visual Science, Institute of Advanced Materials for Nano-Bio Applications, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, China.

Arguments regarding the biocompatibility of graphene-based materials (GBMs) have never ceased. Particularly, the genotoxicity (e.g., DNA damage) of GBMs has been considered the greatest risk to healthy cells. Detailed genotoxicity studies of GBMs are necessary and essential. Herein, we present our recent studies on the genotoxicity of most widely used GBMs such as graphene oxide (GO) and the chemically reduced graphene oxide (RGO) toward human retinal pigment epithelium (RPE) cells. The genotoxicity of GO and RGOs against ARPE-19 (a typical RPE cell line) cells was investigated using the alkaline comet assay, the expression level of phosphorylated p53 determined via Western blots, and the release level of reactive oxygen species (ROS). Our results suggested that both GO and RGOs induced ROS-dependent DNA damage. However, the DNA damage was enhanced following the reduction of the saturated C-O bonds in GO, suggesting that surface oxygen-containing groups played essential roles in the reduced genotoxicity of graphene and had the potential possibility to reduce the toxicity of GBMs via chemical modification.
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http://dx.doi.org/10.1007/s10856-021-06491-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914238PMC
February 2021

Alterations of housekeeping proteins in human aged and diseased hearts.

Pflugers Arch 2021 Mar 26;473(3):351-362. Epub 2021 Feb 26.

Department of Physiology and Biophysics, Rush University Medical Center, 1750 West Harrison St. 1255 Jelke South, Chicago, IL, 60612, USA.

Pathological remodeling includes alterations of ion channel function and calcium homeostasis and ultimately cardiac maladaptive function during the process of disease development. Biochemical assays are important approaches for assessing protein abundance and post-translational modification of ion channels. Several housekeeping proteins are commonly used as internal controls to minimize loading variabilities in immunoblotting protein assays. Yet, emerging evidence suggests that some housekeeping proteins may be abnormally altered under certain pathological conditions. However, alterations of housekeeping proteins in aged and diseased human hearts remain unclear. In the current study, immunoblotting was applied to measure three commonly used housekeeping proteins (β-actin, calsequestrin, and GAPDH) in well-procured human right atria (RA) and left ventricles (LV) from diabetic, heart failure, and aged human organ donors. Linear regression analysis suggested that the amounts of linearly loaded total proteins and quantified intensity of total proteins from either Ponceau S (PS) blot-stained or Coomassie Blue (CB) gel-stained images were highly correlated. Thus, all immunoblotting data were normalized with quantitative CB or PS data to calibrate potential loading variabilities. In the human heart, β-actin was reduced in diabetic RA and LV, while GAPDH was altered in aged and diabetic RA but not LV. Calsequestrin, an important Ca regulatory protein, was significantly changed in aged, diabetic, and ischemic failing hearts. Intriguingly, expression levels of all three proteins were unchanged in non-ischemic failing human LV. Overall, alterations of human housekeeping proteins are heart chamber specific and disease context dependent. The choice of immunoblotting loading controls should be carefully evaluated. Usage of CB or PS total protein analysis could be a viable alternative approach for some complicated pathological specimens.
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http://dx.doi.org/10.1007/s00424-021-02538-xDOI Listing
March 2021

Increased Risk of Infections with Anti-TNF Agents in Patients with Crohn's Disease After Elective Surgery: Meta-Analysis.

Dig Dis Sci 2021 Feb 26. Epub 2021 Feb 26.

Alimentiv Inc. (Formerly Robarts Clinical Trials Inc.), 100 Dundas Street, Suite 200, London, ON, 27N6A 5B6, Canada.

Background: Postoperative complication rates in patients with inflammatory bowel disease (IBD) receiving preoperative biologics have been analyzed without considering the surgical context. Emergency surgery may be associated with an increased risk of infectious complications, compared to elective operations.

Aims: To conduct a systematic review and meta-analysis investigating the relationship between preoperative biologic therapy and postoperative outcomes in Crohn's disease (CD) and ulcerative colitis (UC), focusing on elective surgery.

Methods: Electronic databases were searched up to February 12, 2020, for studies of patients with IBD undergoing elective abdominal surgery receiving biologic therapy within 3 months before surgery compared to no therapy, or another biologic therapy. Certainty of evidence was evaluated using GRADE. The primary outcomes were the rate of infections and total complications within 30 days. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated.

Results: Thirty-three studies were included. Preoperative treatment with anti-tumor necrosis factor (TNF) therapy in patients with CD undergoing elective surgery was associated with increased odds of infection (OR 2.05; 95% CI 1.40-3.01), but not total complications (OR 1.03; 95% CI 0.71-1.51). In elective surgery for UC, preoperative anti-TNF therapy was not associated with infectious (OR 1.03; 95% CI 0.34-3.07) or total complications (OR 0.67; 95% CI 0.29-1.58). Limited data indicate that emergency surgery did not significantly affect the rate of complications.

Conclusions: Anti-TNF therapy prior to elective surgery may increase the odds of postoperative infection in CD, although the certainty of evidence is very low. More evidence is needed, particularly for newer biologics.
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http://dx.doi.org/10.1007/s10620-021-06895-6DOI Listing
February 2021

How Family's Support of Perseverance in Creative Efforts Influences the Originality of Children's Drawing During the Period of COVID-19 Pandemic?

Front Psychol 2021 9;12:600810. Epub 2021 Feb 9.

School of Economics and Management, University of Chinese Academy of Sciences, Beijing, China.

This study points out that families' support of perseverance in creative efforts will increase children's originality of creative drawing through children's persistence in information searching. Data analysis based on 134 Chinese young children's creative drawings and survey supports the above hypothesis. Moreover, children's exposure to COVID-19 pandemic positively moderates the relationship between supporting perseverance and children's search persistence, such that high exposure to COVID-19 pandemic will increase the positive relationship between support of perseverance and search persistence. And children's prosocial motivation inhibits the influence of search persistence on originality. Contributions to the theory of children's creativity are discussed.
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http://dx.doi.org/10.3389/fpsyg.2021.600810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900148PMC
February 2021

Haem relieves hyperoxia-mediated inhibition of HMEC-1 cell proliferation, migration and angiogenesis by inhibiting BACH1 expression.

BMC Ophthalmol 2021 Feb 25;21(1):104. Epub 2021 Feb 25.

Department of Ophthalmology, Xinqiao Hospital, Army Medical University, Xinqiao Road, Shapingba District, Chongqing, 400032, China.

Background: Hyperoxia-mediated inhibition of vascular endothelial growth factor (VEGF) in the retina is the main cause of impeded angiogenesis during phase I retinopathy of prematurity (ROP). Human retinal angiogenesis involves the proliferation, migration and vessel-forming ability of microvascular endothelial cells. Previous studies have confirmed that BTB and CNC homology l (BACH1) can inhibit VEGF and angiogenesis, while haem can specifically degrade BACH1. However, the effect of haem on endothelial cells and ROP remains unknown.

Methods: In this report, we established a model of the relative hyperoxia of phase I ROP by subjecting human microvascular endothelial cells (HMEC-1) to 40% hyperoxia. Haem was added, and its effects on the growth and viability of HMEC-1 cells were evaluated. Cell counting kit 8 (CCK8) and 5-ethynyl-2'-deox-yuridine (EdU) assays were used to detect proliferation, whereas a wound healing assay and Matrigel cultures were used to detect the migration and vessel-forming ability, respectively. Western blot (WB) and immunofluorescence (IF) assays were used to detect the relative protein levels of BACH1 and VEGF.

Results: HMEC-1 cells could absorb extracellular haem under normoxic or hyperoxic conditions. The proliferation, migration and angiogenesis abilities of HMEC-1 cells were inhibited under hyperoxia. Moderate levels of haem can promote endothelial cell proliferation, while 20 μM haem could inhibit BACH1 expression, promote VEGF expression, and relieve the inhibition of proliferation, migration and angiogenesis in HMEC-1 cells induced by hyperoxia.

Conclusions: Haem (20 μM) can relieve hyperoxia-induced inhibition of VEGF activity in HMEC-1 cells by inhibiting BACH1 and may be a potential medicine for overcoming stunted retinal angiogenesis induced by relative hyperoxia in phase I ROP.
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http://dx.doi.org/10.1186/s12886-021-01866-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905865PMC
February 2021

Early Improvement of Acute Respiratory Distress Syndrome in Patients With COVID-19 in the Intensive Care Unit: Retrospective Analysis.

JMIR Public Health Surveill 2021 03 9;7(3):e24843. Epub 2021 Mar 9.

Department of Intensive Care Unit, Chongqing Sixth People's Hospital, Chongqing, China.

Background: Since the start of the COVID-19 pandemic, there have been over 2 million deaths globally. Acute respiratory distress syndrome (ARDS) may be the main cause of death.

Objective: This study aimed to describe the clinical features, outcomes, and ARDS characteristics of patients with COVID-19 admitted to the intensive care unit (ICU) in Chongqing, China.

Methods: The epidemiology of COVID-19 from January 21, 2020, to March 15, 2020, in Chongqing, China, was analyzed retrospectively, and 75 ICU patients from two hospitals were included in this study. On day 1, 56 patients with ARDS were selected for subgroup analysis, and a modified Poisson regression was performed to identify predictors for the early improvement of ARDS (eiARDS).

Results: Chongqing reported a 5.3% case fatality rate for the 75 ICU patients. The median age of these patients was 57 (IQR 25-75) years, and no bias was present in the sex ratio. A total of 93% (n=70) of patients developed ARDS during ICU stay, and more than half had moderate ARDS. However, most patients (n=41, 55%) underwent high-flow nasal cannula oxygen therapy, but not mechanical ventilation. Nearly one-third of patients with ARDS improved (arterial blood oxygen partial pressure/oxygen concentration >300 mm Hg) in 1 week, which was defined as eiARDS. Patients with eiARDS had a higher survival rate and a shorter length of ICU stay than those without eiARDS. Age (<55 years) was the only variable independently associated with eiARDS, with a risk ratio of 2.67 (95% CI 1.17-6.08).

Conclusions: A new subphenotype of ARDS-eiARDS-in patients with COVID-19 was identified. As clinical outcomes differ, the stratified management of patients based on eiARDS or age is highly recommended.
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http://dx.doi.org/10.2196/24843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7945975PMC
March 2021