Publications by authors named "Mei Sun"

477 Publications

Preliminary evidence in treatment of eosinophilic gastroenteritis in children: A case series.

Authors:
Ying Chen Mei Sun

World J Clin Cases 2022 Jul;10(19):6417-6427

Department of Pediatric Gastroenterology, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.

Background: Eosinophilic gastroenteritis is a rare inflammatory disorder in children. However, there is still no standard guideline in the treatment of pediatric eosinophilic gastroenteritis.

Aim: To report our experience with the diagnosis and treatment of children with eosinophilic gastroenteritis.

Methods: From January 2017 to December 2019, a total of 22 children were diagnosed with eosinophilic gastroenteritis.

Results: Endoscopic examination showed eosinophil infiltration in the duodenum [mean number of eosinophils/high-power field (HPF) = 53.1 ± 81.5], stomach (mean number of eosinophils/HPF = 36.8 ± 50.5), and terminal ileum (mean number of eosinophils/HPF = 49.0 ± 24.0). All 18 children with low eosinophil infiltration (< 14%) responded well to the initial drug treatment without relapse, while two of four children with high eosinophil infiltration (> 14%) relapsed after initial methylprednisolone/montelukast treatment. In addition, children with high eosinophil infiltration (> 14%) showed symptomatic relief and histological remission without further relapse after receiving budesonide/methylprednisolone as initial or relapse treatment.

Conclusion: Methylprednisolone/montelukast is still the best treatment for children with low eosinophil infiltration (< 14%). Budesonide can be considered as the initial or relapse treatment for children with high eosinophil infiltration (> 14%).
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http://dx.doi.org/10.12998/wjcc.v10.i19.6417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294883PMC
July 2022

Serum sphingosine-1 phosphate level is increased in patients with hepatitis B and displays a positive association with liver fibrosis.

Am J Transl Res 2022 15;14(7):4964-4976. Epub 2022 Jul 15.

Department of Laboratory Medicine, The Second Xiangya Hospital, Central South University Changsha 410011, Hunan, China.

Objective: To investigate the difference in serum sphingosine-1 phosphate (S1P) concentration between the HBV hepatitis patients and healthy controls, and its relevant association with serum liver fibrosis indicators.

Methods: A total of 28 HBsAg (+) HBeAg (+) Anti-HBc (+) hepatitis B patients, 42 HBsAg (+) Anti-HBe (+) Anti-HBc (+) hepatitis B patients, and 21 healthy subjects with normal liver function were included. Liquid chromatography-tandem mass method was used to detect the level of serum S1P.

Results: SerumS1P concentration of Anti-HBe (+) hepatitis B patients was higher than that of the control group (P=0.017) and HBeAg (+) patients (P=0.007). At the same time, there was no significant difference in the serum S1P concentration between HBeAg (+) hepatitis B patients and the control group (P>0.05). Moreover, serum S1P concentration was positively correlated with liver fibrosis indices, Collage Type IV Protein (r=0.264; P=0.011), and Chitosanase 3-like protein 1 (r=0.295; P=0.004).

Conclusions: Serum S1P level is increased in patients with hepatitis B and displays a positive association with liver fibrosis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360858PMC
July 2022

Does the National Fitness Policy Promote National Health?-An Empirical Study from China.

Int J Environ Res Public Health 2022 Jul 27;19(15). Epub 2022 Jul 27.

College of Public Administration, Huazhong University of Science & Technology, Wuhan 430074, China.

The influence of national health level in the stability and sustainable development of national society is increasingly prominent. The purpose of this study is to examine whether, when, and how national fitness policies exert influence on national health. Panel data from 2008 to 2017 of 30 Chinese provinces (cities) (except the Tibet autonomous region) were used to systematically reveal the direct impact of national fitness policies on national health and its characteristics in different regions, as well as the interaction mechanisms of human capital and finance health expenditures in public sports. This study found that first, national fitness policies had a positive effect on adult health. Second, sports human capital weakens the health effect of national fitness policies, while public finance health expenditures strengthen this effect. Lastly, the health effect of national fitness policies varies significantly across regions due to uneven regional economic development, and the differences in the effects on different age groups (adults and children) are equally pronounced. This study suggests that national fitness public service system and diverse national fitness plans improving national health level are important for a new dynamic balance and high quality coordinated development in both Chinese economic growth and social welfare.
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http://dx.doi.org/10.3390/ijerph19159191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368402PMC
July 2022

Deciphering the role of microplastic size on anaerobic sludge digestion: Changes of dissolved organic matter, leaching compounds and microbial community.

Environ Res 2022 Aug 8;214(Pt 3):114032. Epub 2022 Aug 8.

School of Environmental Science and Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei, 430074, China.

Here the role of microplastic size on dissolved organic matter, leaching compounds and microbial community during anaerobic sludge digestion was evaluated. Compared to that without the addition of polyvinyl chloride (PVC), during the 30 days' incubation, the anaerobic sludge digestion by adding PVC at the size of 75 μm and the concentration of 2.4 g/g volatile solids (VS) showed a 8.5% lower cumulative methane production, while a 17.9% higher cumulative methane production was noted by adding PVC at the size of 3000 μm and the concentration of 2.4 g/g VS. A long-term fed-batch laboratory-scale fermenter test for 147 days further testified, that higher removal efficiencies of total solids, volatile solids, and total chemical oxygen demand, and higher methane production were noted by adding PVC (2.4 g/g VS, 3000 μm) into the fermenter. More interestingly, higher concentrations of proteins, polysaccharides, volatile fatty acids, and soluble microbial by-products component were noted in the liquid phase of sludge drawn from the fermenter added with PVC since the biomass therein showed higher efficiencies of solubilization, hydrolysis, acidification, and methanogenesis. Moreover, as identified from the fermenter added with PVC, dibutyl phthalate (DBP) was the most predominant leaching phthalates compound, although the biomass therein showed a 93.4% anaerobic biodegradability of DBP. The leaching of DBP drove the predominance of microbial community towards Synergistota and Methanosaeta. More irregular elliptical shallow dimples were noted on the PVC surface after 147 days' incubation, accompanied with abundances of Proteobacteria, Actinobacteriota, Chloroflexi, Methanosaeta and Methanobacterium. The results from this study showed that the size of microplastic was a crucial factor in evaluating its impact on anaerobic sludge digestion.
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http://dx.doi.org/10.1016/j.envres.2022.114032DOI Listing
August 2022

The Antidepressant Effect of Deoiled Sunflower Seeds on Chronic Unpredictable Mild Stress in Mice Through Regulation of Microbiota-Gut-Brain Axis.

Front Nutr 2022 1;9:908297. Epub 2022 Jul 1.

Institute of Nutrition and Health, Qingdao University, Qingdao, China.

Objectives: Sunflower seeds provide tryptophan-rich proteins with the potential to protect against depression. Tryptophan is a precursor of serotonin and a substrate for the production of indole derivatives by gut microbiota. This study aimed to investigate the association between the depression-alleviating effects of deoiled and dechlorogenic sunflower seeds (DSFS) and regulation of gut microbiota.

Materials And Methods: Male C57BL/6J mice were fed a diet comprising a source of soy protein (normal and model control), DSFS or whey protein concentrate (positive control) for 7 weeks, and chronic stress-induced depression was induced.

Results: Feeding the DSFS diet prevented depression-like behaviors, intestinal barrier damage, elevated plasma corticosterone, and reduced hippocampal serotonin levels in mice. Meanwhile, Feeding the DSFS diet significantly altered the gut microbiota structure, characterized by elevated relative abundances of , , , and , which were inversely associated with depressive behaviors and markers of mucosal barrier damage. DSFS also altered the gut metabolite profile, prevented depression-induced gut L-tryptophan depletion, and upregulated its metabolite indoleacetaldehyde.

Conclusion: Feeding the DSFS diet prevented depression in mice by remodeling the gut microbiota and bacterial tryptophan metabolism.
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http://dx.doi.org/10.3389/fnut.2022.908297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289741PMC
July 2022

Decorin knockdown is beneficial for aged tendons in the presence of biglycan expression.

Matrix Biol Plus 2022 Aug 24;15:100114. Epub 2022 Jun 24.

McKay Orthopaedic Research Laboratory, University of Pennsylvania, Stemmler Hall, 36th and Hamilton Walk, Philadelphia, PA 19104-6081, United States.

Decorin and biglycan are two major small leucine-rich proteoglycans (SLRPs) present in the tendon extracellular matrix that facilitate collagen fibrillogenesis, tissue turnover, and cell signal transduction. Previously, we demonstrated that knockout of decorin prevented the decline of tendon mechanical properties that are associated with aging. The objective of this study was to determine the effects of decorin and biglycan knockdown on tendon structure and mechanics in aged tendons using tamoxifen-inducible knockdown models. We hypothesized that the knockdown of decorin and compound knockdown of decorin and biglycan would prevent age-related declines in tendon mechanics and structure compared to biglycan knockdown and wild-type controls, and that these changes would be exacerbated as the tendons progress towards geriatric ages. To achieve this objective, we created tamoxifen-inducible mouse knockdown models to target decorin and biglycan gene inactivation without the abnormal tendon development associated with traditional knockout models. Knockdown of decorin led to increased midsubstance modulus and decreased stress relaxation in aged tendons. However, these changes were not sustained in the geriatric tendons. Knockdown in biglycan led to no changes in mechanics in the aged or geriatric tendons. Contrary to our hypothesis, the compound decorin/biglycan knockdown tendons did not resemble the decorin knockdown tendons, but resulted in increased viscoelastic properties in the aged and geriatric tendons. Structurally, knockdown of SLRPs, except for the 570d I- group, resulted in alterations to the collagen fibril diameter relative to wild-type controls. Overall, this study identified the differential roles of decorin and biglycan throughout tendon aging in the maintenance of tendon structural and mechanical properties and revealed that the compound decorin and biglycan knockdown phenotype did not resemble the single gene decorin or biglycan models and was detrimental to tendon properties throughout aging.
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http://dx.doi.org/10.1016/j.mbplus.2022.100114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270257PMC
August 2022

Reversal of right ventricular pressure loading improves biventricular function independent of fibrosis in a rabbit model of pulmonary artery banding.

J Physiol 2022 Aug 21;600(16):3689-3703. Epub 2022 Jul 21.

Division of Cardiology, The Labatt Family Heart Centre, Department of Paediatrics, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.

Right ventricular (RV) pressure loading leads to RV and left ventricular (LV) dysfunction through RV hypertrophy, dilatation and fibrosis. Relief of RV pressure load improves RV function. However, the impact and mechanisms on biventricular reverse-remodelling and function are only partially characterized. We evaluated the impact of RV pressure overload relief on biventricular remodelling and function in a rabbit model of reversible pulmonary artery banding (PAB). Rabbits were randomized to three groups: (1) Sham-operated controls (n = 7); (2) PAB (NDef, n = 7); (3) PAB followed by band deflation (Def, n = 5). Sham and NDef animals were sacrificed at 6 weeks after PAB surgery. Def animals underwent PAB deflation at 6 weeks and sacrifice at 9 weeks. Biventricular geometry, function, haemodynamics, hypertrophy and fibrosis were compared between groups using echocardiography, magnetic resonance imaging, high-fidelity pressure-tipped catheters and histology. RV pressure loading caused RV dilatation, systolic dysfunction, myocyte hypertrophy and LV compression which improved after PAB deflation. RV end-diastolic pressure (RVEDP) decreased after PAB deflation, although remaining elevated vs. Sham. LV end-diastolic pressure (LVEDP) was unchanged following PAB deflation. RV and LV collagen volumes in the NDef and Def group were increased vs. Sham, whereas RV and LV collagen volumes were similar between NDef and Def groups. RV myocyte hypertrophy (r = 0.75, P < 0.001) but not collagen volume was related to RVEDP. LV myocyte hypertrophy (r = 0.58, P = 0.016) and collagen volume (r = 0.56, P = 0.031) correlated with LVEDP. In conclusion, relief of RV pressure overload improves RV and LV geometry, hypertrophy and function independent of fibrosis. The long-term implications of persistent fibrosis and increased biventricular filling pressures, even after pressure load relief, need further study. KEY POINTS: Right ventricular (RV) pressure loading in a pulmonary artery banding rabbit model is associated with RV dilatation, left ventricular (LV) compression; biventricular myocyte hypertrophy, fibrosis and dysfunction. The mechanisms and impact of RV pressure load relief on biventricular remodelling and function has not been extensively studied. Relief of RV pressure overload improves biventricular geometry in conjunction with improved RV myocyte hypertrophy and function independent of reduced fibrosis. These findings raise questions as to the importance of fibrosis as a therapeutic target.
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http://dx.doi.org/10.1113/JP283165DOI Listing
August 2022

The efficiency of a clinical pathway to guide combined applications of interventional pulmonology in undiagnosed pleural effusions.

Sci Rep 2022 Jul 1;12(1):11126. Epub 2022 Jul 1.

Department of Pathology, Dalian Municipal Central Hospital Affiliated of Dalian Medical University, Dalian, 116033, China.

The diagnostic procedure of pleural effusion (PEs) is challenging due to low detection rates and numerous aetiologies. Hence, any attempt to enhance diagnosis is worthwhile. We present a clinical pathway to guide combined application of interventional pulmonology (IP) for tracing causes of undiagnosed PEs. Subjects with undiagnosed PEs were identified in the Hospital Information System of Dalian Municipal Central Hospital from January 1, 2012, to December 31, 2018. Eligible subjects were divided into a group of combined tests and a group of medical thoracoscopy (MT). Optimal and subsequent diagnostic tests were performed depending on the guidance of the clinical pathway by matching profitable chest lesions with the respective adaptation. As the guidance of clinical pathway, common bronchoscopy would be preferentially selected if pulmonary lesions involved or within the central bronchus, EBUS-TBNA was favoured when pulmonary lesions were adjacent to the central bronchus or with the enlarged mediastinal/hilar lymph nodes, guided bronchoscopy would be preferred if pulmonary nodules/masses were larger than 20 mm with discernible bronchus signs, CT-assisted transthoracic core biopsy was preferred if pulmonary nodules were less than 20 mm, image guided cutting needle biopsy was the recommendation if the pleural thickness was larger than 10 mm and pulmonary lesions were miliary. MT was preferred only when undiagnosed PEs was the initial symptom and pulmonary lesions were miliary or absent. A total of 83.57% cases of undiagnosed PEs were eligible for the clinical pathway, and 659 and 216 subjects were included in the combined tests and MT groups, respectively, depending on the optimal recommendation of the clinical pathway. The total diagnostic yields in the combined tests and MT groups were 95.99% and 91.20%, respectively, and the difference in total diagnostic yield was statistically significant (χ = 7.510, p = 0.006). Overall, clinical pathway guidance of the combined application of IP is useful for tracing the causes of undiagnosed PEs. The diagnostic yield of undiagnosed PEs is significantly increased compared with that of MT alone.
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http://dx.doi.org/10.1038/s41598-022-15454-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249795PMC
July 2022

Therapeutic Potential of Curcumin in a Rat Model of Dextran Sulfate Sodium-Induced Ulcerative Colitis by Regulating the Balance of Treg/Th17 Cells.

Inflammation 2022 Jun 22. Epub 2022 Jun 22.

Department of Pediatrics, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, Liaoning, 110004, People's Republic of China.

The pathogenesis of ulcerative colitis (UC) remains unclear, and it is believed that an imbalance of regulatory T (Treg) cells and T helper 17 (Th17) cells is related to the occurrence of UC. Curcumin has been confirmed to exert anti-inflammatory effects in bronchial asthma and osteoarthritis by regulating the balance of Treg/Th17 cells. This study aimed to explore the therapeutic potential of curcumin in dextran sulfate sodium (DSS)-induced UC rats by regulating the balance of Treg/Th17 cells. Disease activity index (DAI) scores were calculated. Changes in colon inflammation were observed using hematoxylin and eosin staining. Treg and Th17 cells in the spleen were detected by flow cytometry, and the levels of interleukin (IL)-10 and IL-17A were determined using enzyme-linked immunosorbent assay. In DSS-induced colitis, curcumin significantly ameliorated colitis symptoms by reducing the DAI and increasing colon length. Additionally, curcumin significantly increased the expression of Treg cells and decreased the expression of Th17 cells and the extent of histopathological damage. Furthermore, curcumin increased the expression of IL-10 and decreased the expression of IL-17A. Curcumin attenuates DSS-induced UC injury by regulating Treg/Th17 balance and related cytokine secretion. Thus, curcumin may be a promising therapeutic drug for treating UC.
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http://dx.doi.org/10.1007/s10753-022-01678-1DOI Listing
June 2022

Effects of long-term organic matter application on soil carbon accumulation and nitrogen use efficiency in a double-cropping rice field.

Environ Res 2022 Oct 16;213:113700. Epub 2022 Jun 16.

Hunan Soil and Fertilizer Institute, Changsha, 410125, PR China.

Increasing soil carbon (C) sequestration in paddy field and improving rice nitrogen use efficiency (NUE) are vital for sustainable agriculture and environmental protection. It was a benefit practice for achieving these goals by taken rice straw and organic manure managements. However, there is still need to further investigate the effects of different long-term fertilizer managements on soil C sequestration and NUE under the double-cropping rice system in southern of China. Therefore, the effects of different long-term (36-years) fertilizer practices on soil C sequestration and NUE under the double-cropping rice system in southern of China were investigated in the present paper. The field experiment was included four different fertilizer treatments: chemical fertilizer alone (MF), rice straw residue and chemical fertilizer (RF), 30% organic manure and 70% chemical fertilizer (OM), and without fertilizer input as a control (CK). This result indicated that soil C content at plough layer in paddy field with RF and OM treatments were increased, compared with MF and CK treatments. Besides input C directly into paddy field, soil original organic C accumulation with RF and OM treatments were increased by 1.54% and 3.01%, compared with MF treatment. This result indicated that soil TOC content increase rate and annual topsoil organic C sequestration rate in paddy field with RF and OM treatments increased by 55.56%, 88.89% and 48.05%, 76.62%, compared with MF treatment, respectively. Compared with MF treatment, NUE with RF and OM treatments increased by 10.43% and 22.61%, respectively, mainly due to increasing soil organic C. Grain yield of double-cropping rice with RF and OM treatments increased by 1009.5 and 1166.5 kg ha, compared with MF treatment, respectively. This result indicated that there was significantly correlation between NUE/NUE and TOC content with RF and OM treatments, at early rice and late rice growth seasons. Therefore, it was benefit practice for increasing soil carbon sequestration and improving rice NUE in the double-cropping rice system with long-term application of rice straw and organic manure managements.
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http://dx.doi.org/10.1016/j.envres.2022.113700DOI Listing
October 2022

Heterogeneous Reaction of Peroxyacetyl Nitrate on Real-World PM Aerosols: Kinetics, Influencing Factors, and Atmospheric Implications.

Environ Sci Technol 2022 07 15;56(13):9325-9334. Epub 2022 Jun 15.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.

The formation and decomposition of peroxyacetyl nitrate (PAN), an important atmospheric nitrogen oxide reservoir, can impact the level and cycling of free radicals and nitrogen compounds in the atmosphere. PAN sinks are poorly understood, highlighting the importance of elucidating the heterogeneous reaction of PAN on aerosol surfaces. Here, we report for the first time the uptake behavior, kinetics, and potential mechanism of PAN uptake on real-world aerosol PM using a flow tube system. The uptake coefficients (γ) of PAN increased non-linearly from (1.5 ± 0.7) × 10 at 0% relative humidity (RH) to (9.3 ± 2.0) × 10 at 80% RH. The γ decrease with increasing initial PAN concentration is consistent with the Langmuir-Hinshelwood mechanism. Organic components of aerosols may promote heterogeneous loss of PAN through redox reactions. Higher γ occurs with higher water content, lower pH, and lower ionic strength in the aqueous phase of aerosols. The present study suggests that heterogeneous reaction of PAN on ambient aerosols plays a non-negligible role in the atmospheric PAN budget and provides new insights into the role of PAN in promoting atmospheric oxidation capacity during hazy periods with cold and wet weather conditions.
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http://dx.doi.org/10.1021/acs.est.2c03050DOI Listing
July 2022

Dynamic monitoring and a clinical correlation analysis of the serum vitamin A, D, and E levels in children with recurrent respiratory tract infections.

Am J Transl Res 2022 15;14(5):3533-3538. Epub 2022 May 15.

Department of Pediatrics, Cangzhou Central Hospital Cangzhou, China.

Objective: To investigate the correlation of the serum vitamin A, D, and E (VA, VD, and VE) levels with the occurrence and development of recurrent respiratory tract infections (RRTIs).

Methods: A total of 129 children with respiratory tract infections (RTIs) treated in our hospital from January 2018 to February 2020 (the RTIs group) and 50 healthy children undergoing physical examinations (the control group) in our hospital were recruited as the study cohort. The serum VA, VD, and VE levels were measured upon admission (the active phase) and at two weeks after discharge (the stable phase). The serum VA, VD, and VE levels in the children with RRTIs were compared with the levels in the control group, and the correlation between these three vitamins and the occurrence and development of RRTIs was analyzed.

Results: The RRTIs group and the RTIs group witnessed markedly lower serum VA, VD, VE, and humoral immunity index levels, including IgG, IgA, and IgM, compared to the control group, with an apparent lower outcome in the RRTIs group than in the RTIs group. The serum levels of the above indexes in the RRTIs children were reduced in the active phase compared with the stable phase. A Pearson correlation analysis showed a positive correlation between VA and IgA. A multivariate logistic regression analysis revealed that a low BMI (Body mass index), prematurity, VA deficiency, VD deficiency, and VE deficiency were the risk factors for RRTIs in children, and outdoor activity was the protective factor.

Conclusion: The VA, VD, and VE levels are closely related to RRTIs in children. It is important to determine and supplement the VA, VD, and VE levels to prevent RTIs in children.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185048PMC
May 2022

Changes of phosphorus species during (hydro) thermal treatments of iron-rich sludge and their solubilization mediated by a phosphate solubilizing microorganism.

Sci Total Environ 2022 Sep 9;838(Pt 4):156612. Epub 2022 Jun 9.

School of Environmental Science and Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.

This study systematically evaluated phosphorus (P) solubilization from pyrochar and hydrochar derived from both raw sludge and iron-rich sludge. The data indicated, that an increase in thermal treatment temperature and the presence of iron promoted the accumulation of P in both pyrochar (derived at 300, 500, and 800 °C) and hydrochar (derived at 100, 200, and 280 °C). After incubating pyrochar and hydrochar with a phosphate solubilizing microorganism (PSM) (Pseudomonas aeruginosa) for 30 days, PSM significantly promoted the solubilization of P in pyrochar and hydrochar synthesized at low temperatures rather than those at high temperatures, with a 59 % increase for the pyrolysis of raw sludge at 300 °C than that pyrolyzed at 800 °C and a 62 % increase for the hydrothermal treatment of raw sludge at 100 °C than that treated at 280 °C. And the phenomena were more obvious on the char samples derived from iron-rich sludge. The mass balance of different P species in the solid and liquid phases indicated that after incubating with PSM for 30 days, NaOH-P was the main P solubilized from the solid phase of pyrochar and HCl-P was the main P solubilized from the solid phase of hydrochar. Considering P availability to plants, the preliminary economic analysis indicated that the hydrothermal treatment of iron-rich sludge at 100 °C showed the highest economic benefits for P recovery, with the net cost of 28.79 USD/ton wet sludge. This study was useful in giving novel insights into the reuse of char samples as P fertilizer, and also suggested the importance of Pseudomonas aeruginosa and other bacteria in sludge application, particularly in terms of P solubilization.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156612DOI Listing
September 2022

Discretion: Whether and How Does It Promote Street-Level Bureaucrats' Taking Charge Behavior?

Front Psychol 2022 24;13:805872. Epub 2022 May 24.

Department of Public Administration, College of Public Administration, Huazhong University of Science and Technology, Wuhan, China.

The extant pieces of literature on discretion has mainly focused on its effect on policy implementation and public service delivery, but few studies have looked at its influence on street-level bureaucrats' work behavior, such as taking charge behavior (TCB), which is of great importance for government reforms, especially in developing and transitional countries. Based on the self-determination theory, this study examines whether and how discretion promotes street-level bureaucrats' TCB. Two studies were conducted among street-level bureaucrats in China. First, a survey experiment ( = 355) suggests that discretion positively predicts street-level bureaucrats' TCB. Then, a survey questionnaire study ( = 442) shows that discretion is positively related to TCB through the mediator of public service motivation (PSM). We concluded with implications for theory and practice.
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http://dx.doi.org/10.3389/fpsyg.2022.805872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9172856PMC
May 2022

[Inhibitory Effect of Interference with PD-L1 Expression on B-cell lymphoma in Mice].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2022 Jun;30(3):771-777

Laboratory of Hematonosis, Northern Jiangsu People's Hospital, Yangzhou Institute of Hematology, Yangzhou 225001, Jiangsu Province, China,E-mail:

Objective: To investigate the potential inhibitory effect of interference with PD-L1 on B cell lymphoma in mice.

Methods: Three shRNA vectors for mouse CD274 (PD-L1) were constructed and transiently transfected into 293T cells. RT-qPCR was used to validate the interference efficiency of CD274. The shRNA vector that interfere efficiently with CD274 expression was packaged by using lentivirus packaging system to generate shRNA lentivirus, and then transfected into A20 lymphoma cell line. The methyl thiazol terazolium (MTT) assay was used to detect proliferation after 48 h culture of CD274-sh A20 cells. Meanwhile, BALB/c mice were hypodermically infected with CD274-sh A20 cells. Infected mice were observed daily and assessed to visualize tumor by in vivo fluorescence imaging.

Results: The proliferation rate of CD274-sh A20 cells in vitro was significantly lower than that of A20 cells (P<0.05). The tumor size detected by in vivo fluorescence imaging showed a significant reduce in tumor bearing mice with CD274-sh compared with other tumor bearing mice. And the weight and size of tumor in CD274-sh group were also significantly reduced compared with other group (P<0.05). Moreover, the survival time of tumor bearing mice in CD274-sh group was longer than that of the PD-L1 high expression group.

Conclusion: PD-L1 plays an important role in the incidence and the progression of lymphoma, and the shRNA-based PD-L1 knockdown can inhibit cell proliferation of A20 cells and partly suppress tumor growth.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2022.03.018DOI Listing
June 2022

Numerical investigation of a squeezing flow between concentric cylinders under the variable magnetic field of intensity.

Sci Rep 2022 Jun 1;12(1):9148. Epub 2022 Jun 1.

Mechanical Engineering Department, College of Engineering, Prince Sattam Bin Abdulaziz University, Wadi ad-Dawasir, 11991, Saudi Arabia.

The ongoing research aims to examine the mass and heat transmission phenomena of squeezing flow between two concentric cylinders under the effect of heat sources and magnetic fields. The impacts of the Lorentz force on the behavior of the liquid flow are elucidated via a magnetic field incorporated in the momentum equation. Furthermore, within concentric cylinders, the expression [Formula: see text] has been employed as a source/sink. The proposed model of PDEs formulates the physical phenomena of time-dependent incompressible two-dimensional squeezing flow via modified Navier-Stokes equation, energy equation, and mass transfer equation, and variable magnetic field. The proposed model involved a highly nonlinear system of PDEs, which has been reduced into a system of ODEs via Lie group of similarity transformation and subsequently solved numerically in MATLAB by Parametric Continuation Method. The direct impact of the squeezing parameter on the profile of temperature and concentration has been observed. The results shown that an increment in the heat source indicates a decline in the liquid temperature profile, that an increment in the heat source indicates a decline in the liquid temperature profile. An increment in the heat source indicates a decline in the liquid temperature prof. At the same time, an inverse relationship is observed for the concentration profile. Therefore, we have witnessed a significant increase in the velocity profiles of the flow, mainly as a result of the heat absorption coefficient. In addition, the declining effect of the Soret number on the concentration profile is noticed. It has been found that it enhanced the entropy generation rate for Pr, [Formula: see text], and Ec, while an opposite impact has been noticed at the Bejan number. The numerical outcomes of the proposed model that explain fluid flow characteristics and fluid flow characteristics are quantitatively elucidated by tables and displayed graphically. The comparison of two numerical results in the cases are found to be in good agreement, as shown in Tables.
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http://dx.doi.org/10.1038/s41598-022-13050-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160035PMC
June 2022

Outcomes of embryo vitrification at different developmental stages: Evaluation of 2412 warming cycles.

Medicine (Baltimore) 2022 May 13;101(19):e29233. Epub 2022 May 13.

Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.

Introduction: Advances in cell culture media have led to a shift from cleavage stage embryo transfer to blastocyst stage transfer. Extended embryo culture to the blastocyst stage provides some theoretical advantages and disadvantages. There has been controversy. This study is sought to evaluate the clinical outcomes of vitrified-warmed cleavage-stage and blastocyst-stage embryo transfers in patients undergoing Artificial Reproductive Technique treatments.

Material And Method: The study was performed on 2740 women undergoing frozen embryo thawing transfer. Patients' basic situation, status of frozen embryo transfer cycle, clinical pregnancy rate, early abortion rate, sex ratio of birth, and birth weight were retrospectively analyzed. We compared the main clinical results of recovery of frozen embryo on the cleavage stage and blastocyst stage. Furthermore, we evaluated the clinical outcomes of blastocyst cryopreservation on Day 5, 6, or 7 after oocyte retrieval according to the day of blastocyst expansion were evaluated.

Results: The implantation ratio of cleavage stage embryos was 21.62% compared with 43.52% on D5 (P < .05). The D5, D6, and D7 implanting rates were statistically different. The pregnancy rates were 57.56%, 51.76%, and 35.95% versus 37.79%, respectively for embryos cryopreserved on D5, D6, D7, and D3. The ectopic pregnancy rate and early abortion rate were statistically different between D5 and D3. The sex ratio, the birth weight, and birth defect were not statistically different among the four groups.

Conclusions: Blastocyst transfer achieved a higher implantation rate than vitrified cleavage stage embryo and decreased ectopic pregnancy rate. With increased incubation days before expansion blastocyst formed, the implantation rate is reduced and the early abortion rate increases.
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http://dx.doi.org/10.1097/MD.0000000000029233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276305PMC
May 2022

Pathways from Neuroticism, Social Support, and Sleep Quality to Antenatal Depression during the Third Trimester of Pregnancy.

Int J Environ Res Public Health 2022 05 5;19(9). Epub 2022 May 5.

Xiangya School of Nursing, Centreal South University, 172 Tongzipo Road, Changsha 410013, China.

: Antenatal depression is a severe public health problem. Many studies support the concept that neuroticism, social support, and sleep quality are closely related to antenatal depression. However, there is little evidence concerning the influencing pathways of these variables on antenatal depression. The aim of this study is to investigate the pathways from neuroticism, social support, and sleep quality to antenatal depression during the third trimester of pregnancy. : A cross-sectional study design was used. A total of 773 eligible women in the third trimester of pregnancy submitted valid questionnaires from June 2016 to April 2017. Instruments with good reliability and validity were used to measure neuroticism, social support, sleep quality, and antenatal depression. Structural equation modeling was used to explore the pathways from neuroticism, social support, and sleep quality to antenatal depression during the third trimester of pregnancy. : Antenatal depression is shown to be positively correlated with neuroticism and negatively correlated with social support and sleep quality. Neuroticism is shown to have a direct effect and indirect effects through social support and sleep quality on antenatal depression. : Neuroticism influences antenatal depression directly and indirectly. Social support and sleep quality are the mediators of the indirect relationship between neuroticism and antenatal depression. Our results suggest that a personality test offered to all pregnant women could help detect a vulnerability to depression, whereupon intervention in the domains of sleep and social support could prove preventive.
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http://dx.doi.org/10.3390/ijerph19095602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100278PMC
May 2022

Prevalence, awareness, and associated factors of high blood pressure among female migrant workers in Central South China.

PeerJ 2022 4;10:e13365. Epub 2022 May 4.

Department of Family Health Care Nursing, School of Nursing, University of California, San Francisco, CA, United States of America.

Background: Although many young Chinese women migrate to urban regions for better opportunities, little is known about the prevalence and awareness of having high blood pressure (HBP) in this population. This study investigated the prevalence, awareness, and factors associated with HBP among young female migrant workers in Central South China.

Methods: We conducted a cross-sectional study to identify HBP (2017 ACC/AHA guidelines) among female migrant workers aged 18-45 years in Central South China. Demographics, anthropometric measurements, hypertension-related lifestyle, awareness of HBP, and blood pressure were recorded. Logistic regression analysis was used to identify the factors associated with HBP (blood pressure ≥ 130/80 mmHg).

Results: Overall, 232 female migrants participated in the study (mean age 34.4; standard deviation: 6.4 years). The prevalence of HBP was 27.2% (95% CI [21.6-33.2]), and 88.9% of the participants were unaware of their HBP status. Having rural medical insurance (odds ratio [OR] = 20.7; 95% confidence interval 95% CI [2.1-204.8]), awareness of having HBP (OR = 5.1; 95% CI [1.4-18.5]), physical inactivity (OR = 2.9; 95% CI [1.1-7.9]), and being overweight/obese (OR = 2.7; 95% CI [1.3-6.1]) were independently associated with HBP.

Conclusions: This study revealed a high prevalence of HBP among young Chinese female migrant workers, as well as a high frequency of being unaware of their condition and some associated factors (rural medical insurance, awareness of having HBP, physical inactivity, and overweight/obesity). The uncontrolled HBP among young Chinese female migrant workers suggested that health education needs further promotion in such a population.
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http://dx.doi.org/10.7717/peerj.13365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078134PMC
May 2022

[Upper gastrointestinal ulcer in children: a clinical analysis of 173 cases].

Authors:
En-Hui Wang Mei Sun

Zhongguo Dang Dai Er Ke Za Zhi 2022 Apr;24(4):372-376

Department of Pediatric Gastroenterology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110000, China.

Objectives: To study the clinical manifestations and gastroscopic characteristics of upper gastrointestinal ulcer in children.

Methods: A retrospective analysis was performed for the children who underwent gastroscopy and were found to have upper gastrointestinal ulcer for the first time at the Endoscopy Center of Shengjing Hospital, China Medical University, from January 2011 to May 2021. According to the cause of the disease, they were divided into primary ulcer group (primary group; =148) and secondary ulcer group (secondary group; =25). The clinical data were compared between the two groups.

Results: A total of 173 children with upper gastrointestinal ulcer were enrolled, with a male/female ratio of 3.9:1. Compared with girls, boys had significantly higher proportions of duodenal ulcer and primary ulcer (<0.05). Compared with the children aged below 6 years, the children aged 6-14 years had higher proportions of duodenal ulcer and primary ulcer and lower proportions of giant ulcer and multiple ulcers. Of the 148 children in the primary group, 95 (64.2%) had infection. Abdominal pain was the most common clinical symptom and was observed in 101 children (68.2%). Duodenal ulcer was common and was observed in 115 children (77.7%), followed by gastric ulcer in 25 children (16.9%) and esophageal ulcer in 7 children (4.7%). Multiple ulcers were observed in 32 children (21.6%). Seventy children (47.3%) experienced complications, among which bleeding was the most common complication and was observed in 63 children (43.6%). Of the 25 children in the secondary group, abdominal pain was the most common clinical symptom and was observed in 9 children (36.0%), with a significantly lower incidence rate than the primary group (<0.05); foreign body in the digestive tract was the most common cause of ulcer and was observed in 17 children (68%), followed by abdominal Henoch-Schönlein purpura in 5 children (20.0%) and Crohn's disease in 3 children (12.0%). The secondary group had a significantly higher proportion of multiple ulcer or giant ulcer than the primary group (<0.05).

Conclusions: Upper gastrointestinal ulcer is more common in boys than girls, and duodenal ulcer and primary ulcer are more common in boys. Children aged 6-14 years often have duodenal ulcer and primary ulcer, and giant ulcer and multiple ulcers are relatively uncommon. Primary ulcer in children has a variety of clinical manifestations, mainly abdominal pain, and duodenal ulcer is relatively common, with bleeding as the main complication. The clinical symptoms and endoscopic manifestations of secondary ulcer are closely associated with the primary causes, and it is more likely to induce huge ulcers and multiple ulcers.
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http://dx.doi.org/10.7499/j.issn.1008-8830.2201003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044988PMC
April 2022

Single cell mass spectrometry studies reveal metabolomic features and potential mechanisms of drug-resistant cancer cell lines.

Anal Chim Acta 2022 May 1;1206:339761. Epub 2022 Apr 1.

Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, 73019, USA. Electronic address:

Irinotecan (Iri) is a key drug to treat metastatic colorectal cancer, but its clinical activity is often limited by de novo and acquired drug resistance. Studying the underlying mechanisms of drug resistance is necessary for developing novel therapeutic strategies. In this study, we used both regular and irinotecan-resistant (Iri-resistant) colorectal cell lines as models, and performed single cell mass spectrometry (SCMS) metabolomics studies combined with analyses from cytotoxicity assay, western blot, flow cytometry, quantitative real-time polymerase chain reaction (qPCR), and reactive oxygen species (ROS). Our SCMS results indicate that Iri-resistant cancer cells possess higher levels of unsaturated lipids compared with the regular cancer cells. In addition, multiple protein biomarkers and their corresponding mRNAs of colon cancer stem cells are overexpressed in Iri-resistance cells. Particularly, stearoyl-CoA desaturase 1 (SCD1) is upregulated with the development of drug resistance in Iri-resistant cells, whereas inhibiting the activity of SCD1 efficiently increase their sensitivity to Iri treatment. In addition, we demonstrated that SCD1 directly regulates the expression of ALDH1A1, which contributes to the cancer stemness and ROS level in Iri-resistant cell lines.
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http://dx.doi.org/10.1016/j.aca.2022.339761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046687PMC
May 2022

Development and Validation of a Risk Score Screening Tool to Identify People at Risk for Hypertension in Shanghai, China.

Risk Manag Healthc Policy 2022 30;15:553-562. Epub 2022 Mar 30.

Department of Health Policy and Management, School of Public Health, Fudan University, Shanghai, People's Republic of China.

Purpose: This study aimed to develop a screening tool based on a risk scoring approach that could identify individuals at high risk for hypertension in Shanghai, China.

Methods: A total of 3147 respondents from the 2013 Shanghai Chronic Disease and Risk Factor Surveillance were randomly divided into the derivation group and validation group. The coefficients obtained from multivariable logistic regression were used to assign a score to each variable category. The receiver operating characteristic (ROC) curve was used to find the optimal cut-off point and to evaluate the screening performance.

Results: Age, family history of hypertension, having diabetes, having dyslipidemia, body mass index, and having abdominal obesity contributed to the risk score. The area under the ROC curve was 0.817 (95% CI: 0.797-0.836). The optimal cut-off value of 20 had a sensitivity of 83.4%, and a specificity of 64.3%, demonstrating good performance.

Conclusion: We developed a simple and valid screening tool to identify individuals at risk for hypertension. Early detection could be beneficial for high-risk groups to better manage their conditions and delay the progression of hypertension and related complications.
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http://dx.doi.org/10.2147/RMHP.S354057DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977866PMC
March 2022

Internalised stigma, social support, coping and care-giving burden among Chinese family caregivers of adults diagnosed with schizophrenia: A parallel mediation analysis.

Health Soc Care Community 2022 Mar 30. Epub 2022 Mar 30.

Xiangya School of Nursing, Central South University, Changsha, China.

Care-giving burden and internalised stigma are prevalent among family caregivers of people diagnosed with schizophrenia. Internalised stigma has been regarded as a source of care-giving burden. But it remains unclear if high levels of internalised stigma directly contribute to an increased risk of care-giving burden or if the effects could be buffered by psychological factors. This cross-sectional study was to investigate the relationship between internalised stigma and care-giving burden, and to determine the mediating effects of coping styles and social support. Data were collected from 344 Chinese family caregivers of adults diagnosed with schizophrenia in a psychiatric outpatient department of a tertiary hospital in Changsha, Hunan between April and August 2018. A self-reported questionnaire was used to collect data anonymously. Instruments included the Simplified Coping Style Questionnaire, the Multidimensional Scale of Perceived Social Support, the Internalized Stigma of Mental Illness Scale and the Caregiver Burden Inventory. Data analysis was conducted using descriptive statistics, the Spearman correlation and regression analysis to estimate direct and indirect effects using bootstrap analysis. Results showed that internalised stigma, social support and passive coping were significant correlates of care-giving burden; social support partially mediated the relationship between internalised stigma and care-giving burden; active coping did not show impacts on internalised stigma and care-giving burden. This study provided social workers and healthcare providers with a better understanding of the development of care-giving burden. Comprehensive interventions should be designed to provide supportive resources and reduce the possibilities of internalisation of stigma and passive coping, to alleviate care-giving burden.
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http://dx.doi.org/10.1111/hsc.13780DOI Listing
March 2022

Therapeutic endoscopy of a Dieulafoy lesion in a 10-year-old girl: A case report.

World J Clin Cases 2022 Feb;10(6):1966-1972

Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.

Background: There are multiple causes of sudden gastrointestinal bleeding in children. Reports of Dieulafoy lesions (DLs) in children are scarce. DLs can be fatal without appropriate treatment.

Case Summary: We present a retrospective analysis of the clinical manifestations, endoscopic features, and treatment of a Chinese girl with a DL, as well as a review of the relevant literature. A 10-year-old girl was admitted to our hospital with sudden massive hematemesis and melena. Abdominal computed tomography revealed suspected submucosal bleeding in the stomach. Finally, the disease was diagnosed with endoscopy due to the typical manifestations. We used electrocoagulation and hemoclips under endoscopy for hemostasis. No recurrence of hematemesis was identified during 4-wk' follow-up.

Conclusion: DLs in children are rare but an important cause of sudden gastrointestinal hemorrhage. Many pediatricians are inexperienced and often miss or delay diagnosis. Endoscopy as early as possible is the first choice for diagnosis and treatment.
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http://dx.doi.org/10.12998/wjcc.v10.i6.1966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8891784PMC
February 2022

Single cell mass spectrometry analysis of drug-resistant cancer cells: Metabolomics studies of synergetic effect of combinational treatment.

Anal Chim Acta 2022 Apr 24;1201:339621. Epub 2022 Feb 24.

Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, 73019, USA. Electronic address:

Irinotecan (IRI), a topoisomerase I inhibitor blocking DNA synthesis, is a widely used chemotherapy drug for metastatic colorectal cancer. Despite being an effective chemotherapy drug, its clinical effectiveness is limited by both intrinsic and acquired drug resistance. Previous studies indicate IRI induces cancer stemness in irinotecan-resistant (IRI-resistant) cells. Metformin, an oral antidiabetic drug, was recently reported for anticancer effects, likely due to its selective killing of cancer stem cells (CSCs). Given IRI-resistant cells exhibiting high cancer stemness, we hypothesize metformin can sensitize IRI-resistant cells and rescue the therapeutic effect. In this work, we utilized the Single-probe mass spectrometry technique to analyze live IRI-resistant cells under different treatment conditions. We discovered that metformin treatment was associated with the downregulation of lipids and fatty acids, potentially through the inhibition of fatty acid synthase (FASN). Importantly, certain species can be only detected from cells in their living status. The level of synergistic effect of metformin and IRI in their co-treatment of IRI-resistant cells was evaluated using Chou-Talalay combinational index. Using enzymatic activity assay, we determined that the co-treatment exhibit the highest FASN inhibition compared with the mono-treatment of IRI or metformin. To our knowledge, this is the first single-cell MS metabolomics study demonstrating metformin-IRI synergistic effect overcoming drug resistance in IRI-resistant cells.
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http://dx.doi.org/10.1016/j.aca.2022.339621DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8933618PMC
April 2022

Dexmedetomidine alleviates the hypoxic-ischemic brain damage via miR-20a-5p/methionine adenosyltransferase 2B axis in rat pups.

Neuroreport 2022 03;33(5):205-214

Department of Anesthesiology, Southern Theater General Hospital of PLA, Guangzhou City, Guangdong Province, China.

Objective: The neuroprotective effect of dexmedetomidine (DEX) has been demonstrated in hypoxic-ischemic brain damage (HIBD) animal models, the mechanism of which will be the foothold in this work.

Methods: After establishment of HIBD rat model, the rats were treated with DEX, miR-20a-5p agomir and adenoviral methionine adenosyltransferase 2B (MAT2B) overexpression vector, and then their brain tissues were harvested. The infarction volume and pathological changes of these brain tissues were measured using the triphenyl tetrazolium chloride (TTC), Nissl and hematoxylin-eosin (HE) stainings. The levels of miR-20a-5p, Bcl-2, Bax and MAT2B in these brain tissues were detected by Real-Time PCR (RT-PCR) and western blot. The binding sites of MAT2B and miR-20a-5p were predicted using the TargetScan and verified using the dual-luciferase reporter assay. The memory deficits and spatial learning of rat pups were assessed by Morris water maze test.

Results: MiR-20a-5p expression was upregulated, while MAT2B expression was downregulated in rats with HIBD. MAT2B was targeted by miR-20a-5p. DEX treatment improved the neurons and hippocampal tissue damage and decreased miR-20a-5p level in brain tissues of rats with HIBD. MiR-20a-5p overexpression overturned the protective effect of DEX on brain tissues and learning and memory abilities in rats with HIBD. Moreover, DEX promoted Bcl-2 level while inhibiting Bax level in HIBD rats' brain tissues. Besides, overexpressed MAT2B reversed the effect of overexpressed miR-20a-5p on the levels of MAT2B, Bcl-2 and Bax, brain tissue damage, as well as the learning and memory abilities in rats with HIBD.

Conclusion: DEX alleviated HIBD via the miR-20a-5p/MAT2B axis in rats.
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http://dx.doi.org/10.1097/WNR.0000000000001750DOI Listing
March 2022

Increased Levels of CHI3L1 and HA Are Associated With Higher Occurrence of Liver Damage in Patients With Obstructive Sleep Apnea.

Front Med (Lausanne) 2022 25;9:854570. Epub 2022 Feb 25.

Department of Laboratory Medicine, The Second Xiangya Hospital of Central South University, Changsha, China.

Background: Obstructive sleep apnea-hypopnea syndrome (OSA) may cause liver fibrosis, and liver fibrosis serum biomarkers plays an important role on the diagnosis of liver fibrosis. In addition, this study aimed to observe the changes of 4 serum markers and Chitinase 3-like protein 1 (CHII3L1) levels in OSA patients with different disease severity and explore their interactions. And then, we examined whether intermittent hypoxia (IH) exposure can activate hepatic stellate cell.

Methods: 74 OSA patients in Second Xiangya hospital from January 2021 to October 2021 was selected and categorized into mild, moderate, and severe groups according to AHI. In addition, 20 subjects were selected as the control group. Serum levels of liver fibrosis markers were determined by electrochemiluminescence immunoassay. Hepatic stellate cells were exposed to intermittent IH or normoxia (RA). Results were analyzed using the SPSS software.

Results: There was a significant increase in serum hyaluronic acid (HA), collagen type IV (CIV) and CHI3L1 levels in OSA patients compared with control group. Specifically, serum liver fibrosis markers HA, CIV and CHI3L1 levels were positively correlated with apnea-hypopnea index (AHI), but negatively correlated with the lowest saturation oxygen (LSaO) respectively. The LX-2 cells (human hepatic stellate cell line) exposed to IH showed significant increases in fibrotic protein expression.

Conclusion: OSA might either directly or indirectly trigger or exacerbate liver fibrosis, possibly via IH-related pathways.
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http://dx.doi.org/10.3389/fmed.2022.854570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913888PMC
February 2022

Probiotics combined with zinc and selenium preparation in the treatment of child rotavirus enteritis.

Am J Transl Res 2022 15;14(2):1043-1050. Epub 2022 Feb 15.

Department of Pediatrics, Cangzhou Central Hospital Cangzhou 061000, Hebei Province, China.

Objective: To investigate clinical effect of probiotics combined with zinc and selenium preparation in the treatment of child rotavirus enteritis.

Methods: In this retrospective study, the patients were divided into two groups based on treatment method. The control group (n=42) received probiotic therapy, while the experimental group (n=43) received probiotics combined with zinc and selenium preparation. Clinical efficacy, stool frequency and incidence of adverse reactions after treatment were compared to assess the clinical effect.

Results: The clinical effect was improved after intervention (P<0.05), and the total effective rate of two groups was 88.4% (38/43), 50% (21/42), respectively. Time to symptom disappearance was significantly decreased in the experimental group as compared to the control group. The myocardial zymogram indices (CK, CK-MB and AST) were decreased after treatment, and the levels in the experimental group were significantly lower than in the control group (P<0.05). Moreover, we observed that the levels of inflammatory factors (IL-6, IL-8 and hsCRP) in the experimental group were significantly lower than those of the control group after intervention (all P<0.05).

Conclusion: Probiotics combined with zinc and selenium preparation can not only significantly improve the clinical effect, but also shorten the course of disease.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8902533PMC
February 2022

Numerical Simulation of a Time-Dependent Electroviscous and Hybrid Nanofluid with Darcy-Forchheimer Effect between Squeezing Plates.

Nanomaterials (Basel) 2022 Mar 6;12(5). Epub 2022 Mar 6.

Department of Pure and Applied Mathematics, University of Haripur, Haripur 22620, Khyber Pakhtunkhwa, Pakistan.

In this article, the behavior of transient electroviscous fluid flow is investigated through squeezing plates containing hybrid nanoparticles. A hybrid nanofluid MoS2+Au/C2H6O2-H2O was formulated by dissolving the components of an inorganic substance such as molybdenum disulfide (MoS2) and gold (Au) in a base fluid of ethylene glycol/water. This hybrid non-liquid flow was modeled by various nonlinear mathematical fluid flow models and subsequently solved by numerical as well as analytical methods. For the numerical solution of nonlinear ODEs, a built-in function BVP4C was used in MATLAB, and the same problem was solved in MATHEMATICA by HAM. The result of the present problem related to the results obtained from the existing literature under certain conditions. The outcomes revealed that the concentration profiles were more sensitive to homogeneity diversity parameters. The simulation of the various physical parameters of the model indicated that the heat transfer through a mixture of hybrid nanofluids was greater than a simple nanofluid. In addition, the phenomenon of mixed convection was considered to improve the velocity of simple nanofluids and hybrid nanofluids, when both cases have low permeability. A rise in the volume fraction of the nanomaterials, Φ, was associated with an increase in the heat transfer rate. It was observed that the heat transfer rate of the hybrid nanofluids MoS2+Au/C2H6O2-H2O was higher than that of the single nanofluids MoS2/C2H6O2-H2O.
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http://dx.doi.org/10.3390/nano12050876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912341PMC
March 2022

Brain-gut-microbiota axis in Parkinson's disease: A historical review and future perspective.

Brain Res Bull 2022 06 1;183:84-93. Epub 2022 Mar 1.

Department of Neurology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. Electronic address:

Parkinson's disease (PD) is the second most common degenerative disease of the central nervous system (CNS) after Alzheimer's disease. In addition to the typical motor symptoms, the clinical manifestations of patients with PD include gastrointestinal symptoms, which even precede the motor symptoms. Recent research has found that the gut microbiota regulates the brain-gut axial interaction through immune, endocrine, and direct neural mechanisms, supporting the hypothesis that the pathological process of PD spreads from the gut to the brain. In this review article, we highlight the landmark findings in the field of PD, with particular attention to the brain-gut-microbiota axis. We summarize the changes and their clinical effects on the gut microbiota and metabolites observed in PD. The intestinal microbiota may contain appropriate targets for the prevention and treatment of PD. Clinical cohort studies suggest that certain intestinal microbes have protective or pathogenic effects on the progression of PD. A better understanding of the interaction between the gut-brain axis, the gut microbiota, and PD has the potential to lead to new diagnostic and therapeutic approaches. Animal experiments suggest that fecal microbiota transplantation (FMT) is helpful for treating PD, and FMT is expected to be an effective treatment for PD in the future.
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http://dx.doi.org/10.1016/j.brainresbull.2022.02.015DOI Listing
June 2022
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