Publications by authors named "Mei Li"

1,974 Publications

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Introduction of probiotic bacterial consortia promotes plant growth via impacts on the resident rhizosphere microbiome.

Proc Biol Sci 2021 Oct 13;288(1960):20211396. Epub 2021 Oct 13.

Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, Key Lab of Plant immunity, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Weigang 1, Nanjing 210095, People's Republic of China.

Plant growth depends on a range of functions provided by their associated rhizosphere microbiome, including nutrient mineralization, hormone co-regulation and pathogen suppression. Improving the ability of plant-associated microbiomes to deliver these functions is thus important for developing robust and sustainable crop production. However, it is yet unclear how beneficial effects of probiotic microbial inoculants can be optimized and how their effects are mediated. Here, we sought to enhance tomato plant growth by targeted introduction of probiotic bacterial consortia consisting of up to eight plant-associated strains. We found that the effect of probiotic consortium inoculation was richness-dependent: consortia that contained more strains reached higher densities in the tomato rhizosphere and had clearer beneficial effects on multiple plant growth characteristics. Crucially, these effects were best explained by changes in the resident community diversity, composition and increase in the relative abundance of initially rare taxa, instead of introduction of plant-beneficial traits into the existing community along with probiotic consortia. Together, our results suggest that beneficial effects of microbial introductions can be driven indirectly through effects on the diversity and composition of the resident plant rhizosphere microbiome.
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http://dx.doi.org/10.1098/rspb.2021.1396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511750PMC
October 2021

Rapid conversion of IgG to biosensor using an antibody-binding protein-based probe.

Analyst 2021 Oct 11;146(20):6114-6118. Epub 2021 Oct 11.

Key Laboratory for Antibodies Medicine, School of Life Science and Technology, Weifang Medical University, Weifang 261053, China.

We successfully developed a fluorescent probe that can quickly convert full-length antibodies to Quenchbodies, which represent a type of fluorescent immunosensor with high binding affinity and specificity depending on the reaction of antigens and antibodies. An anti-testosterone IgG was successfully converted to an immunosensor that detects testosterone with a limit of detection (LOD) of 0.76 nM and concentration for 50% of maximal effect (EC) of 61.5 nM. Another IgG-based immunosensor detected ractopamine with an LOD of 15.5 pM and EC of 48.6 nM.
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http://dx.doi.org/10.1039/d1an01171eDOI Listing
October 2021

Conditional PD-1/PD-L1 Probody therapeutics induce comparable antitumor immunity but reduced systemic toxicity compared with traditional anti-PD 1/PD-L1 agents.

Cancer Immunol Res 2021 Oct 11. Epub 2021 Oct 11.

Research, CytomX Therapeutics, Inc.

Immune checkpoint blockade has revolutionized cancer treatment. However, most patients do not respond to single-agent therapy. Combining checkpoint inhibitors with other immune-stimulating agents increases both efficacy and toxicity due to systemic T-cell activation. Protease-activatable antibody prodrugs, known as Probody therapeutics (Pb-Tx), localize antibody activity by attenuating capacity to bind antigen until protease activation in the tumor microenvironment. Herein, we show that systemic administration of anti-programmed cell death ligand 1 (anti-PD-L1) and anti-programmed cell death protein 1 (anti-PD-1) Pb-Tx to tumor-bearing mice elicited antitumor activity similar to that of traditional PD 1/PD-L1-targeted antibodies. Pb-Tx exhibited reduced systemic activity and an improved nonclinical safety profile, with markedly reduced target occupancy on peripheral T cells and reduced incidence of early-onset autoimmune diabetes in nonobese diabetic mice. Our results confirm that localized PD-1/PD-L1 inhibition by Pb-Tx can elicit robust antitumor immunity and minimize systemic immune-mediated toxicity. These data provide further preclinical rationale to support the ongoing development of the anti-PD-L1 Pb-Tx CX-072, which is currently in clinical trials.
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http://dx.doi.org/10.1158/2326-6066.CIR-21-0031DOI Listing
October 2021

Association of the Monocyte-to-High-Density Lipoprotein Cholesterol Ratio With Diabetic Retinopathy.

Front Cardiovasc Med 2021 21;8:707008. Epub 2021 Sep 21.

Department of Endocrinology & Metabolism, The Third Affiliated Hospital of Sun Yat-sen University, Guangdong Provincial Key Laboratory of Diabetology, Guangzhou, China.

Chronic inflammation in type 2 diabetes mellitus (T2DM) is an essential contributor to the development of diabetic retinopathy (DR). The monocyte-to-high-density lipoprotein cholesterol (HDL-C) ratio (MHR) is a novel and simple measure related to inflammatory and oxidative stress status. However, little is known regarding the role of the MHR in evaluating the development of DR. A total of 771 patients with T2DM and 607 healthy controls were enrolled in this cross-sectional study. MHR determination and eye examination were performed. The association of MHR with the prevalence of DR in T2DM patients was analyzed. The MHR in patients with DR was significantly higher than that in both non-DR diabetic patients ( < 0.05) and healthy controls ( < 0.01). No significance was observed in the MHR of different DR severity grades. Moreover, the MHR was similar between patients with non-macular oedema and those with macular oedema. Logistic regression analysis demonstrated that MHR was independently associated with the prevalence of DR in diabetic patients [odds ratio (OR) = 1.438, 95% confidence interval (CI): 1.249-1.655, < 0.01]. After additional stratification by HbA1c level and diabetic duration, the MHR was still independently associated with the prevalence of DR. Our study suggests that the MHR can be used as a marker to indicate the prevalence of DR in patients with T2DM.
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http://dx.doi.org/10.3389/fcvm.2021.707008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490616PMC
September 2021

Electroacupuncture and Moxibustion Modulate the BDNF and TrkB Expression in the Colon and Dorsal Root Ganglia of IBS Rats with Visceral Hypersensitivity.

Evid Based Complement Alternat Med 2021 28;2021:8137244. Epub 2021 Sep 28.

Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.

Objective: To evaluate the effects of electroacupuncture and moxibustion on brain-derived neurotrophic factor (BDNF) and its receptor tyrosine kinase receptor B (TrkB) protein and mRNA expressions in the colon and dorsal root ganglia of IBS rats with visceral hypersensitivity and to explore their underlying therapeutic mechanisms.

Method: Forty Sprague Dawley rats were randomly divided into normal, model, model + mild moxibustion (MM), model + electroacupuncture (EA), and model + pinaverium bromide (PB) groups, with eight rats in each group. Chronic visceral hypersensitive IBS rat models were established by colorectal distension (CRD) with mustard oil clyster. Rats in the MM and EA groups, respectively, received moxibustion and electroacupuncture treatments on the Tianshu (ST25) and Shangjuxu (ST37) acupoints once daily for 7 days, and rats in the PB group received pinaverium bromide by oral gavage once daily for 7 consecutive days. After treatment, rats underwent abdominal withdrawal reflex (AWR) scoring under CRD and colon histopathological examination. Immunohistochemistry and real-time quantitative PCR (RT-qPCR) were used to study the protein and mRNA expressions of BDNF and TrkB in the rat colon and dorsal root ganglia.

Results: Compared with the normal group, AWR scores and body weight were clearly increased in the model group rats (both < 0.01). The body weights were significantly elevated ( < 0.01, < 0.05), but the AWR scores were reduced ( < 0.05, < 0.01), after electroacupuncture and mild moxibustion treatment. Compared with levels in normal rats, BDNF and TrkB protein and mRNA expressions were significantly elevated in the IBS model rats ( < 0.01) but were downregulated after mild moxibustion, electroacupuncture, and Western medicine treatment ( < 0.01).

Conclusion: Electroacupuncture and moxibustion improved visceral hypersensitivity of IBS rats possibly by reducing BDNF and TrkB protein and mRNA expressions in the colon and dorsal root ganglia.
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http://dx.doi.org/10.1155/2021/8137244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492290PMC
September 2021

OsFes1C, a potential nucleotide exchange factor for OsBiP1, is involved in the ER and salt stress responses.

Plant Physiol 2021 Sep;187(1):396-408

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100093, China.

The endoplasmic reticulum (ER) quality control system monitors protein homeostasis and relies on the activity of many molecular chaperones. Binding immunoglobulin protein (BiP) is a major ER luminal chaperone that is involved in most functions of the organelle. BiP activity is tightly regulated by nucleotide exchange factors (NEFs). However, information about NEFs in plants is limited. We obtained a Fes1-like protein (OsFes1C) through isobaric tags for relative and absolute quantitation-based proteomics analysis of ER-stressed rice (Oryza sativa) seeds. Unlike its homologs in yeast and mammals, which are located in the cytosol and respond to heat stress, OsFes1C is an ER membrane protein and responds to ER and salt stresses. OsFes1C interacts directly with OsBiP1 and the interaction is inhibited by ATP but promoted by ADP, suggesting that OsFes1C acts as a potential NEF of OsBiP1 in vivo. Overexpression or suppression of OsFes1C led to hypersensitivity to ER stress and affected the growth of rice. Furthermore, we established that OsFes1C directly interacts with a putative salt response protein and is involved in the salt response. Taken together, our study marks an important step toward elucidating the functional mechanisms of an identified ER stress response factor in rice.
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http://dx.doi.org/10.1093/plphys/kiab263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418431PMC
September 2021

Pure Mg-Al Layered Double Hydroxide Film on Magnesium Alloys for Orthopedic Applications.

ACS Omega 2021 Sep 16;6(38):24575-24584. Epub 2021 Sep 16.

Department of Orthopedics, Research Center of Medical Sciences, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, China.

Mg alloys are promising biodegradable orthopedic implants in the future. However, poor corrosion resistance and biocompatibility limit their wide applications. In this study, a pure Mg-Al layered double hydroxide (Mg-Al LDH) film on AZ31 was prepared through combining hydrofluoric acid pretreatment and hydrothermal treatment. Electrochemical analysis and the immersion test suggested that the as-prepared Mg-Al LDH-coated sample exhibited significantly enhanced corrosion resistance. The cell culture revealed that the Mg-Al LDH film was favorable for the alkaline phosphatase activity, collagen secretion, and osteogenesis-related gene expression of MC3T3-E1. Furthermore, the LDH-coated sample was beneficial for the migration, vascular endothelial growth factor secretion, and angiogenesis-related gene expression of human umbilical vein endothelial cells. The subcutaneous implantation test demonstrated that the Mg-Al LDH film could protect the substrate from corrosion and induce milder inflammation. The femur implantation demonstrated that the Mg-Al LDH sample showed better bone regeneration and osseointegration than bare AZ31. In summary, the as-prepared pure Mg-Al LDH film is able to enhance the and performances of AZ31, indicating a promising application in the orthopedic field.
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http://dx.doi.org/10.1021/acsomega.1c03169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482458PMC
September 2021

Exposure to money modulates the processing of charitable donation: An event-related potentials study.

Neurosci Lett 2021 Sep 28;765:136277. Epub 2021 Sep 28.

Department of Psychology, Hunan Normal University, Changsha, Hunan, China; Cognition and Human Behavior Key Laboratory of Hunan Province, China. Electronic address:

Exposure to monetary cues might affect charitable donations, but little is known about how monetary cues affect charitable donations from the neural perspective. The present study examined the effect of monetary cues on charitable donations by means of event-related potentials (ERPs). Participants primed with monetary or neutral images decided whether to accept donation offers with the high, moderate, and low costs. The behavioural results showed that in the money-primed condition, participants took more time to decide for the high-cost than for the moderate and low-cost donation offers. The ERP results showed that the P2 and P3 were larger in the money-primed condition relative to the neutral images condition. Notably, participants primed with money demonstrated larger P3 for the high-cost donation offers than for the moderate and low-cost offers, but this difference was not observed in the control condition. These findings indicate that people primed with money may pay more attention to the cost-relevant information related to their self-interests when conducting prosocial behaviours.
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http://dx.doi.org/10.1016/j.neulet.2021.136277DOI Listing
September 2021

Effect of Surgical Masks on Cardiopulmonary Function in Healthy Young Subjects: A Crossover Study.

Front Physiol 2021 10;12:710573. Epub 2021 Sep 10.

Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Mask plays an important role in preventing infectious respiratory diseases. The influence of wearing masks in physical exercise on the human body needs to be studied. The purpose of this study is to explore the influence of wearing surgical masks on the cardiopulmonary function of healthy people during exercise. The physiological responses of 71 healthy subjects (35 men and 36 women, age 27.77 ± 7.76 years) to exercises with and without surgical masks (mask-on and mask-off) were analyzed. Cardiopulmonary function and metabolic reaction were measured by the cardiopulmonary exercise test (CPET). All tests were carried out in random sequence and should be completed in 1 week. The CPETs with the mask-on condition were performed undesirably ( < 0.05), and the Borg scale was higher than the mask-off ( < 0.001). Rest oxygen uptake ( ) and carbon dioxide production ( CO) with the mask-on condition were lower than mask-off ( < 0.01), which were more obvious at peak exercise ( O : 1454.8 ± 418.9 vs. 1628.6 ± 447.2 ml/min, < 0.001; CO : 1873.0 ± 578.7 vs. 2169.9 ± 627.8 ml/min, = 0.005), and the anaerobic threshold (AT) brought forward ( < 0.001). At different stages of CPET with the mask-on condition, inspiratory and expiratory time (Te) was longer ( < 0.05), and respiratory frequency (Rf) and minute ventilation ( ) were shorter than mask-off, especially at peak exercise (Rf : 33.8 ± 7.98 vs. 37.91 ± 6.72 b/min, < 0.001; : 55.07 ± 17.28 vs. 66.46 ± 17.93 l/min, < 0.001). was significantly lower than mask-off just at peak exercise (1.66 ± 0.45 vs. 1.79 ± 0.5 l, < 0.001). End-tidal oxygen partial pressure (PetO), end-tidal carbon dioxide partial pressure (PetCO), oxygen ventilation equivalent ( / O), and carbon dioxide ventilation equivalent ( / CO) with mask-on, which reflected pulmonary ventilation efficiency, were significantly different from mask-off at different stages of CPET ( < 0.05), but no significant difference in percutaneous oxygen saturation (SpO) was found. Differences in oxygen pulse ( O/HR), oxygen uptake efficiency slope (OUES), work efficiency (△ O/△W), peak heart rate (HR), and peak systolic blood pressure (BP) existed between two conditions ( < 0.05). Wearing surgical masks during aerobic exercise showed certain negative impacts on cardiopulmonary function, especially during high-intensity exercise in healthy young subjects. These results provide an important recommendation for wearing a mask at a pandemic during exercises of varying intensity. Future research should focus on the response of wearing masks in patients with related cardiopulmonary diseases.
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http://dx.doi.org/10.3389/fphys.2021.710573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461071PMC
September 2021

Identification and phylogenetic analysis of voltage-gated sodium channel haplotypes in the malaria vector Anopheles sinensis using a high-throughput amplicon sequencing approach.

Parasit Vectors 2021 Sep 26;14(1):499. Epub 2021 Sep 26.

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

Background: Anopheles sinensis is a dominant vector for malaria transmission in Asian countries. Voltage-gated sodium channel (VGSC) mutation-mediated knock-down resistance (kdr) has developed in many A. sinensis populations because of intensive and long-term use of pyrethroids. Our previous study showed that multiple mutations at position 1014 of the VGSC were heterogeneously distributed in A. sinensis populations across Sichuan, China.

Methods: To understand resistance genotypes at the haplotype level and reconstruct the phylogenetic relationship of VGSC haplotypes, a cost-effective next-generation sequencing (NGS)-based amplicon sequencing approach was established to clarify haplotypes containing codon 1014 of the VGSC gene from a total of 446 adults collected in 12 locations of Sichuan, China.

Results: Nineteen (19) haplotypes were identified, including 11 wild 1014L, 6 resistance 1014F, and 2 resistance 1014C haplotypes. We found that resistance haplotypes of A. sinensis VGSC were widely distributed at frequencies ranging from 3.67 to 92.61%. The frequencies of the 1014C haplotype in the southeast of Sichuan (Luzhou, Guangan, and Suining) were relatively higher than those in other sampling locations. Phylogenetic analyses support that kdr-type mutation at position 1014 is not singly originated and resistance 1014C haplotypes evolve from TTT-encoding 1014F.

Conclusions: A cost-effective next-generation sequencing (NGS)-based amplicon sequencing approach has been established in this study. The data revealed the patchy distribution of VGSC resistance haplotypes with overall high frequencies in Sichuan, China. Phylogenetic analyses support multiple origins and sequential evolution (1014L → 1014F → 1014C) for kdr-type mutations in A. sinensis.
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http://dx.doi.org/10.1186/s13071-021-05009-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474825PMC
September 2021

A Nomogram for the Determination of the Necessity of Concurrent Chemotherapy in Patients With Stage II-IVa Nasopharyngeal Carcinoma.

Front Oncol 2021 6;11:640077. Epub 2021 Sep 6.

Department of Radiation Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu, China.

Background: The efficiency of concurrent chemotherapy (CC) remains controversial for stage II-IVa nasopharyngeal carcinoma (NPC) patients treated with induction chemotherapy (IC) followed by intensity-modulated radiotherapy (IMRT). Therefore, we aimed to propose a nomogram to identify patients who would benefit from CC.

Methods: A total of 434 NPC patients (stage II-IVa) treated with IC followed by IMRT between January 2010 and December 2015 were included. There were 808 dosimetric parameters extracted by the in-house script for each patient. A dosimetric signature was developed with the least absolute shrinkage and selection operator algorithm. A nomogram was built by incorporating clinical factors and dosimetric signature using Cox regression to predict recurrence-free survival (RFS). The C-index was used to evaluate the performance of the nomogram. The patients were stratified into low- and high-risk recurrence according to the optimal cutoff of risk score.

Results: The nomogram incorporating age, TNM stage, and dosimetric signature yielded a C-index of 0.719 (95% confidence interval, 0.658-0.78). In the low-risk group, CC was associated with a 9.4% increase of 5-year locoregional RFS and an 8.8% increase of 5-year overall survival (OS), whereas it was not significantly associated with an improvement of locoregional RFS (LRFS) and OS in the high-risk group. However, in the high-risk group, patients could benefit from adjuvant chemotherapy (AC) by improving 33.6% of the 5-year LRFS.

Conclusions: The nomogram performed an individualized risk quantification of RFS in patients with stage II-IVa NPC treated with IC followed by IMRT. Patients with low risk could benefit from CC, whereas patients with high risk may require additional AC.
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http://dx.doi.org/10.3389/fonc.2021.640077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450530PMC
September 2021

Predictive value of early amplitude integrated electroencephalogram (aEEG) in sleep related problems in children with perinatal hypoxic-ischemia (HIE).

BMC Pediatr 2021 Sep 18;21(1):410. Epub 2021 Sep 18.

Soochow Key Laboratory of Prevention and Treatment of Child Brain Injury, Children's Hospital of Soochow University, No.92 Zhongnanjie Road, Suzhou, 215025, P.R. China.

Background: While great attention has been paid to motor and cognitive impairments in children with neonatal Hypoxic-Ischemic Encephalopathy (HIE), sleep related circadian rhythm problems, although commonly present, are often neglected. Subsequently, no early clinical indicators have been reported to correlate with sleep-related circadian dysfunction during development.

Methods: In this study, we first analyzed patterns of the amplitude integrated electroencephalogram (aEEG) in a cohort of newborns with various degrees of HIE. Next, during follow-ups, we collected information of sleep and circadian related problems in these patients and performed correlation analysis between aEEG parameters and different sleep/circadian disorders.

Results: A total of 101 neonates were included. Our results demonstrated that abnormal aEEG background pattern is significantly correlated with circadian rhythmic (r = 0.289, P = 0.01) and breathing issues during sleep (r = 0.237, P = 0.037). In contrast, the establishment of sleep-wake cycle (SWC) showed no correlation with sleep/circadian problems. Detailed analysis showed that summation of aEEG score, along with low base voltage (r = 0.272, P = 0.017 and r = -0.228, P = 0.048, respectively), correlates with sleep circadian problems. In contrast, background pattern (BP) score highly correlates with sleep breathing problem (r = 0.319, P = 0.004).

Conclusion: Abnormal neonatal aEEG pattern is correlated with circadian related sleep problems. Our study thus provides novel insights into predictive values of aEEG in sleep-related circadian problems in children with HIE.
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http://dx.doi.org/10.1186/s12887-021-02796-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449491PMC
September 2021

Association of Hypertension with Mild Cognitive Impairment in Population from Less-Developed Areas of Multiethnic Northwest China.

Neuroepidemiology 2021 25;55(5):407-415. Epub 2021 Aug 25.

Hypertension Center of People's Hospital of Xinjiang Uygur Autonomous Region, Xinjiang Hypertension Institute, National Health Committee Key Laboratory of Hypertension Clinical Research, Urumqi, China.

Background: Relationship between hypertension and mild cognitive impairment (MCI) remains undetermined in population from less-developed regions. We aimed to explore whether hypertension is associated with MCI in this specific population.

Methods: In this cross-sectional study, we enrolled subjects aged ≥18 years using multistage random sampling from Emin, China, in 2019. Participants underwent questionnaires and data collection including mini-mental state examination (MMSE) and blood pressure measurement.

Results: Finally, 31,329 subjects were included, with 11,270 hypertensives. Compared with normotensive subjects, hypertensives were characterized by significantly older age (55.19 ± 12.25 vs. 43.26 ± 12.71), more men (52.5% vs. 42.9%), low education attainment (≤primary education: 42.4% vs. 26.3%), more abdominal obesity (39.7% vs. 19.1%), poor sleep quality (39.1% vs. 28.7%), and chronic kidney disease (6.6% vs. 3.4%, p for all <0.001). Prevalence of MCI in hypertensives was significantly higher than that of normotensive subjects (24.3% vs. 15.6%, p < 0.001). Multivariate logistic regression analysis showed in a fully adjusted model that the odds for MCI were significantly increased in hypertensives than in normotensive population (OR = 1.19, 95% CI: 1.09, 1.30, p < 0.001) and independent of all the parameters studied including age, education level, and stroke. In the age-stratified regression model, presence of hypertension significantly increased the odds of MCI by 1.17-fold (95% CI: 1.03, 1.33, p = 0.020) and by 1.22-fold (95% CI: 1.04, 1.44, p = 0.016) in middle-aged and elderly population. Sensitivity analysis of excluding those with stroke history showed that hypertension was still a risk factor for MCI in total, middle-aged, and elderly population.

Conclusion: Hypertension is in independent negative association with MCI in middle-aged and elderly population from underdeveloped regions.
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http://dx.doi.org/10.1159/000517956DOI Listing
August 2021

Role of MRI-Based Functional Imaging in Improving the Therapeutic Index of Radiotherapy in Cancer Treatment.

Front Oncol 2021 27;11:645177. Epub 2021 Aug 27.

Department of Gynecology and Obstetrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, China.

Advances in radiation technology, such as intensity-modulated radiation therapy (IMRT), have largely enabled a biological dose escalation of the target volume (TV) and reduce the dose to adjacent tissues or organs at risk (OARs). However, the risk of radiation-induced injury increases as more radiation dose utilized during radiation therapy (RT), which predominantly limits further increases in TV dose distribution and reduces the local control rate. Thus, the accurate target delineation is crucial. Recently, technological improvements for precise target delineation have obtained more attention in the field of RT. The addition of functional imaging to RT can provide a more accurate anatomy of the tumor and normal tissues (such as location and size), along with biological information that aids to optimize the therapeutic index (TI) of RT. In this review, we discuss the application of some common MRI-based functional imaging techniques in clinical practice. In addition, we summarize the main challenges and prospects of these imaging technologies, expecting more inspiring developments and more productive research paths in the near future.
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http://dx.doi.org/10.3389/fonc.2021.645177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429950PMC
August 2021

MFG-E8 accelerates wound healing in diabetes by regulating "NLRP3 inflammasome-neutrophil extracellular traps" axis.

Cell Death Discov 2020 Sep 10;6(1):84. Epub 2020 Sep 10.

Department of Clinical Laboratory, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Sustained activation of NLRP3 inflammasome and release of neutrophil extracellular traps (NETs) impair wound healing of diabetic foot ulcers (DFUs). Our previous study reported that milk fat globule epidermal growth factor VIII (MFG-E8) attenuates tissue damage in systemic lupus erythematosus. However, the functional effect of MFG-E8 on "NLRP3 inflammasome-NETs" inflammatory loop in wound healing of diabetes is not completely elucidated. In this study, neutrophils from DFU patients are susceptible to undergo NETosis, releasing more NETs. The circulating levels of NET components neutrophil elastase and proteinase 3 and inflammatory cytokines IL-1β and IL-18 were significantly elevated in DFU patients compared with healthy controls or diabetic patients, in spite of higher levels of MFG-E8 in DFU patients. In Mfge8 diabetic mice, skin wound displayed exaggerated inflammatory response, including leukocyte infiltration, excessive activation of NLRP3 inflammasome (release of higher IL-1β, IL-18, and TNF-α), largely lodged NETs, resulting in poor angiogenesis and wound closure. When stimulated with high-dose glucose or IL-18, MFG-E8-deficient neutrophils release more NETs than WT neutrophils. After administration of recombinant MFG-E8, IL-18-primed NETosis of WT or Mfge8 neutrophils was significantly inhibited. Furthermore, NET and mCRAMP (component of NETs, the murine equivalent of cathelicidin LL-37 in human)-mediated activation of NLRP3 inflammasome and production of IL-1β/IL-18 were significantly elevated in Mfge8 macrophages compared with WT macrophages, which were also significantly dampened by the administration of rmMFG-E8. Therefore, our study demonstrated that as inhibitor of the "NLRP3 inflammasome-NETs" inflammatory loop, exogenous rMFG-E8 improves angiogenesis and accelerates wound healing, highlighting possible therapeutic potential for DFUs.
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http://dx.doi.org/10.1038/s41420-020-00318-7DOI Listing
September 2020

Protective effect of parecoxib sodium against ischemia reperfusion‑induced intestinal injury.

Authors:
Mei Li Zhi Zheng

Mol Med Rep 2021 Nov 9;24(5). Epub 2021 Sep 9.

Department of Anesthesiology, Huangyan Hospital Affiliated to Wenzhou Medical University, Taizhou First People's Hospital, Taizhou, Zhejiang 318020, P.R. China.

Ischemia reperfusion (I/R)‑induced intestinal injury is a pathophysiological process leading to oxidative stress and inflammatory responses, and revealing its underlying mechanisms is essential for developing therapeutic strategies. Cyclooxygenase (COX) has been reported to be involved in I/R injury. Parecoxib sodium, a selective inhibitor for COX‑2, exerts protective effects, such as reducing I/R‑induced injuries in the heart, kidney and brain. However, the potential role of parecoxib sodium in protecting the small intestine against I/R‑induced injury has rarely been investigated. Therefore, the aim of the present study was to elucidate the effects and potential mechanisms of parecoxib sodium in I/R‑induced intestinal injury. In total, 60 Sprague‑Dawley rats were randomly divided into four groups: Control (sham operation) group, intestinal I/R group, 10 mg/kg parecoxib sodium‑pre‑treated I/R (I/R + Pare/10) group and the 20 mg/kg parecoxib sodium‑pre‑treated I/R (I/R + Pare/20) group. A regular I/R model was established to induce the intestinal injury in rats. Parecoxib sodium at 10 or 20 mg/kg was intraperitoneally administered into rats in both I/R + Pare groups once daily for 5 consecutive days prior to ischemia. Blood samples and small intestinal tissues were collected at 2 h after reperfusion. Changes in the levels of malondialdehyde, nitric oxide, interleukin (IL)‑1β, IL‑8, intercellular cell adhesion molecule‑1 and IL‑10, as well as the total antioxidant capacity were determined using ELISA, as were the activities of superoxidase dismutase and myeloperoxidase. Furthermore, the protein expression levels of total caspase‑3, cleaved caspase‑3, Bcl‑2 and Bax were examined via western blot analysis. In addition, the daily survival rate post‑reperfusion was examined for 7 days. It was revealed that parecoxib sodium increased the levels of antioxidants and suppressed the intestinal oxidative injury induced by I/R. Moreover, parecoxib sodium downregulated the expression levels of the proinflammatory factors, but upregulated the expression levels of anti‑inflammatory factors. The results also demonstrated that parecoxib sodium attenuated I/R‑induced apoptosis and increased the survival rate of rats. Thus, administration of parecoxib sodium prior to intestinal I/R attenuated intestinal injury and increased the rat survival rate by inhibiting I/R‑induced inflammation, oxidative stress and apoptosis.
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http://dx.doi.org/10.3892/mmr.2021.12416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436217PMC
November 2021

Design and Preparation Technology of Single/Dual-Cross-Linking Copolymers Based on Oil.

ACS Omega 2021 Aug 19;6(34):21915-21924. Epub 2021 Aug 19.

Institute of Chemical Industry of Forestry Products, CFA, Nanjing 210042, Jiangsu Province, China.

To fully utilize the forestry bioresources, a novel dual-cross-linkable resin monomer of MAHSWAGMA was prepared from oil (SWO). FT-IR and H-NMR analysis demonstrated the successful synthesis of the target product. Five different cross-linking copolymers, including a polymerized vinyl ester/single-cross-linking (PVESC) polymer, a polymerized epoxy/single-cross-linking (PESC) copolymer, a thermal-photo-initiated/dual-cross-linking (TPIDC) copolymer, a photo-thermal-initiated/dual-cross-linking copolymer (PTIDC), and a thermal-initiated/dual-cross-linking copolymer (TIDC), were obtained with different preparation technologies by different initiated cross-linking processes. Thermal and mechanical properties of the five copolymers were all tested, and the effects of different preparation technologies on the properties of prepared copolymers were investigated. The prepared three dual-cross-linking copolymers had higher hardness, relative cross-linking density, glass transition temperature, and more excellent mechanical property than the other two single-cross-linking copolymers. The PTIDC copolymerized system obtained with photo first and thermal latter initiated dual-cross-linking preparation technology had the most excellent comprehensive properties. This study can provide an ideal idea for the design and preparation of dual-cross-linking copolymers based on forestry vegetable oil.
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http://dx.doi.org/10.1021/acsomega.1c02199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8412939PMC
August 2021

Xenon Nanobubbles for the Image-Guided Preemptive Treatment of Acute Ischemic Stroke via Neuroprotection and Microcirculatory Restoration.

ACS Appl Mater Interfaces 2021 Sep 8;13(37):43880-43891. Epub 2021 Sep 8.

School of Biomedical Engineering and Informatics, Nanjing Medical University, Nanjing 211166, P. R. China.

Early lesion site diagnosis and neuroprotection are crucial to the theranostics of acute ischemic stroke. Xenon (Xe), as a nontoxic gaseous neuroprotectant, holds great promise for ischemic stroke therapy. In this study, Xe-encapsulated lipid nanobubbles (Xe-NBs) have been prepared for the real-time ultrasound image-guided preemptive treatment of the early stroke. The lipids are self-assembled at the interface of free Xe bubbles, and the mean diameter of Xe-NBs is 225 ± 11 nm with a Xe content of 73 ± 2 μL/mL. The results show that Xe-NBs can protect oxygen/glucose-deprived PC12 cells against apoptosis and oxidative stress. Based on the ischemic stroke mice model, the biodistribution, timely ultrasound imaging, and the therapeutic effects of Xe-NBs for stroke lesions were investigated . The accumulation of Xe-NBs to the ischemic lesion endows ultrasound contrast imaging with the lesion area. The cerebral blood flow measurement indicates that the administration of Xe-NBs can improve microcirculatory restoration, resulting in reduced acute microvascular injury in the lesion area. Furthermore, local delivery of therapeutic Xe can significantly reduce the volume of cerebral infarction and restore the neurological function with reduced neuron injury against apoptosis. Therefore, Xe-NBs provide a novel nanosystem for the safe and rapid theranostics of acute ischemic stroke, which is promising to translate into the clinical management of stroke.
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http://dx.doi.org/10.1021/acsami.1c06014DOI Listing
September 2021

De novo mutation in COL2A1 leads to lethal foetal skeletal dysplasia.

Bone 2021 Dec 4;153:116169. Epub 2021 Sep 4.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China; Clinical Center of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. Electronic address:

Objective: Skeletal dysplasia caused by genetic mutations places a heavy burden on families and society. This study was performed to precise diagnosis of variants of unknown significance and to expand the genotypic spectrum of lethal skeletal dysplasia.

Methods: According to the ultrasonic phenotype of the proband and whole-exome sequencing results, variation sites or genes that may be related to the disease were screened out. We verified the accuracy of the variation site through Sanger sequencing. Using bioinformatics, zebrafish models, and assisted reproduction technology (ART) combined with preimplantation genetic testing for monogenic diseases, the disease-causing mutation was verified.

Results: A missense mutation (c.3944G>A, p.Cys1315Tyr) was found in the coding region of COL2A1. Although the mutation is a variant of unknown significance, it is highly conserved and was predicted to be harmful by the SIFT and PolyPhen-2 software. In contrast to the control group, col2a1a mutation-expressing zebrafish larvae showed significant spinal curvature. Through preimplantation genetic testing for monogenic diseases excluding the missense mutation, a child conceived by ART was birthed with normal bone development.

Conclusion: We identified a de novo mutation in human COL2A1 related to lethal skeletal dysplasia and expanded the mutation spectrum of type II collagenopathies. In addition, we provided a new strategy based on a zebrafish model and ART for patients who harbour variants of unknown significance to have a healthy baby without genetic disease similar to the proband.
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http://dx.doi.org/10.1016/j.bone.2021.116169DOI Listing
December 2021

Ultrasonication enhanced the multi-scale structural characteristics of rice starch following short-chain fatty acids acylation.

Int J Biol Macromol 2021 Nov 4;190:333-342. Epub 2021 Sep 4.

College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China; ARC Functional Grains Centre, Charles Sturt University, Wagga Wagga, NSW 2678, Australia. Electronic address:

Considering the variation of the diffusion character of the three anhydrides, ultrasonication was applied for investigating its impact on the reaction efficiency of the rice starch acylation from three short-chain fatty acids (SCFAs). The current data indicated that the signal peak of the FTIR spectrum at 1720 cm and additional resonances in the NMR confirmed the occurrence of the acylation reaction onto the starch molecules. More interestingly, this is the first study to reveal that a lower power density ultrasonication improved the reaction efficiencies of acetylation (19%), while a higher power density could lead to a reduced acylation reactivity of propionylation compared to the control one. On the contrary, the reaction efficiency of butyrylation (64%) was significantly enhanced by the ultrasound-assisted treatment with a greater association between reaction efficiency and ultrasonic power density, indicating the importance of the diffusion character for impacting the acylation reactivity among these three anhydrides. The ultrasonic-assisted SCFAs-modified rice starch has a lower peak viscosity and setback value, indicating that the replacement of the acyl groups for OH groups in the starch avoids starch molecules rearrangement. Meanwhile, the rheological properties exhibited that the starch achieved from ultrasonic-assistance significantly reduced the area of the hysteresis curve, suggesting a destroyed gel textural property. Thus, an appropriate ultrasonication but not all could effectively enhance the acylation efficiency and improve starch rheological property.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.08.227DOI Listing
November 2021

Pregabalin and Gabapentin in Patients with Spinal Cord Injury-Related Neuropathic Pain: A Network Meta-Analysis.

Pain Ther 2021 Sep 7. Epub 2021 Sep 7.

Department of Neurology, Gongren Hospital, Tangshan, 063000, Hebei, China.

Introduction: This study was performed to explore the efficacy and safety of pregabalin and gabapentin in patients with spinal cord injury (SCI)-induced neuropathic pain to determine which treatment is most suitable for such patients.

Methods: We searched the PubMed, MEDLINE, Embase, and Cochrane Library databases from database inception to August 31, 2020. The quality of the included studies was assessed. We selected the average pain intensity after treatment and the proportion of patients who discontinued treatment because of adverse effects as the outcome indicators for efficacy and safety, respectively. Statistical analyses were performed using Stata, v16.0, and RevMan, v5.3, software.

Results: We included eight randomized controlled trials that examined four interventions (pregabalin, gabapentin, carbamazepine, and amitriptyline). Based on the average pain intensity after treatment, the efficacy order from highest to lowest was pregabalin, gabapentin, amitriptyline, carbamazepine, and placebo. Based on the proportion of patients who discontinued treatment because of adverse effects, the order from highest to lowest was pregabalin, amitriptyline, carbamazepine, gabapentin, and placebo. In addition, five studies reported the overall incidence of treatment-related adverse effects for two interventions (pregabalin and gabapentin). According to the pooled analysis of these studies, the order for the overall incidence of treatment-related adverse effects from highest to lowest was pregabalin, gabapentin, and placebo.

Conclusions: This study revealed that for patients with SCI-related neuropathic pain, pregabalin was the most effective for relieving pain, whereas gabapentin performed better in aspects associated with drug therapy-related safety.
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http://dx.doi.org/10.1007/s40122-021-00302-8DOI Listing
September 2021

Alteration of Bone Density, Microarchitecture, and Strength in Patients with Camurati-Engelmann Disease: Assessed by HR-pQCT.

J Bone Miner Res 2021 Sep 7. Epub 2021 Sep 7.

Department of Endocrinology, Key Laboratory of Endocrinology, NHC, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Camurati-Engelmann disease (CED) is a rare autosomal-dominant skeletal dysplasia caused by mutations in the transforming growth factor-β1 (TGFB1) gene. In this study, a retrospective review of patients with CED evaluated at Peking Union Medical College Hospital in Beijing, China, between November 30, 2000 and November 30, 2020 was conducted. Data including demographic data, manifestations, and examination results were characterized. Furthermore, bone geometry, density, and microarchitecture were assessed and bone strength was estimated by HR-pQCT. Results showed the median age at onset was 2.5 years. Common manifestations included pain in the lower limbs (94%, 17/18), abnormal gait (89%, 16/18), genu valgum (89%, 16/18), reduced subcutaneous fat (78%, 14/18), delayed puberty (73%, 8/11), muscle weakness (67%, 12/18), hearing loss (39%, 7/18), hepatosplenomegaly (39%, 7/18), exophthalmos or impaired vision or visual field defect (33%, 6/18), and anemia (33%, 7/18). Twenty-five percent (4/16) of patients had short stature. Serum level of alkaline phosphatase was elevated in 41% (7/17) of patients whereas beta-C-terminal telopeptide was elevated in 91% of patients (10/11). Among 12 patients, the Z-scores of two patients were greater than 2.5 at the femur neck and the Z-scores of five patients were lower than -2.5 at the femur neck and/or lumbar spine. HR-pQCT results showed lower volumetric BMD (vBMD), altered bone microstructure and lower estimated bone strength at the distal radius and tibia in patients with CED compared with controls. In addition, total volume bone mineral density and cortical volumetric bone mineral density at the radius were negatively correlated with age in patients with CED, but positively correlated with age in controls. In conclusion, the largest case series of CED with characterized clinical features in a Chinese population was reported here. In addition, HR-pQCT was used to investigate bone microstructure at the distal radius and tibia in nine patients with CED, and the alteration of bone density, microstructure, and strength was shown for the first time. © 2021 American Society for Bone and Mineral Research (ASBMR).
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http://dx.doi.org/10.1002/jbmr.4436DOI Listing
September 2021

USP5 Sustains the Proliferation of Glioblastoma Through Stabilization of CyclinD1.

Front Pharmacol 2021 16;12:720307. Epub 2021 Aug 16.

Institute of Pediatric Research, Children's Hospital of Soochow University, Suzhou, China.

Glioblastoma multiforme (GBM) is one of the most malignant primary tumors in humans. Despite standard therapeutic strategy with tumor resection combined with radiochemotherapy, the prognosis remains disappointed. Recently, deubiquitinating enzymes (DUBs) has been reported as potential cancer therapy targets due to their multifunctions involved in the regulation of tumorigenesis, cell cycle, apoptosis, and autophagy. In this study, we found that knockdown of ubiquitin specific protease (USP5), a family member of DUB, could significantly suppress GBM cell line U251 and DBTRG-05MG proliferation and colony formation by inducing cell cycle G1/S arrest, which was correlated with downregulation of CyclinD1 protein level. CyclinD1 had been reported to play a critical role in the tumorigenesis and development of GBM regulating cell cycle transition. Overexpression of USP5 could significantly extend the half-life of CyclinD1, while knockdown of USP5 decreased the protein level of CyclinD1, which could be restored by proteasome inhibitor MG-132. Indeed, USP5 was found to directly interact with CyclinD1, and decrease its K48-linked polyubiquitination level. Furthermore, knockdown of USP5 in U251 cells remarkably inhibited tumor growth . Taken together, these findings demonstrate that USP5 plays a critical role in tumorigenesis and progression of GBM by stabilizing CyclinD1 protein. Targeting USP5 could be a potential therapeutic strategy for GBM.
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http://dx.doi.org/10.3389/fphar.2021.720307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415357PMC
August 2021

Cryptogenic Stroke Caused by Pulmonary Arterial Venous Malformation with Massive Right-to-Left Shunt: A Case Report.

Neurol Ther 2021 Sep 3. Epub 2021 Sep 3.

Research Team for Acupuncture Effect and Mechanism, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China.

Background: Pulmonary arterial venous malformation (PAVM) is an abnormal vascular malformation between pulmonary arteries and veins characterized by varying degrees of right-to-left shunts (RLS). Cryptogenic stroke (CS) due to paradoxical embolism (PE) caused by PAVM is relatively rare in the clinic.

Case Presentation: We report the case of a 54-year-old right-handed woman who presented with sudden-onset left-sided limb weakness for 2 h. A physical examination revealed normal vital signs but weakness in her left upper and lower limbs, graded as 1/5 using the Medical Research Council scale. Her National Institutes of Health Stroke Scale (NIHSS) score was 8, and her modified Rankin scale (mRS) was 4. Brain diffusion-weighted imaging showed acute infarction in the right basal ganglia and the radiation crown but brain magnetic resonance angiography found no obvious abnormality. A transcranial Doppler ultrasound with bubble study (TCD-b) found the rain curtain sign of microbubbles in the left middle cerebral artery, reflecting significant RLS. Transesophageal echocardiography (TEE) and transthoracic echocardiography (TTE) were conducted to distinguish between intra- and extracardiac shunts. A pulmonary computerized tomography angiogram (CTA) demonstrated a PAVM. We considered the patient had CS due to PE caused by PAVM. Thrombolytic therapy within the time window was performed. Then, transcatheter device occlusion of the arteriovenous fistula was successfully undertaken, and the patient carried on with rehabilitation training. At a 15-month follow-up, there were no catheter-related complications or recurrent stroke, and her NIHSS and mRS scores were both 0.

Conclusions: PAVM is an important risk factor for PE and CS and should not be ignored as a possible etiology in stroke patients without any other risk factors. CTA of the pulmonary artery is the recommended gold standard for diagnosing and locating a PAVM. Thrombolytic therapy within the time window combined with transcatheter device occlusion of arteriovenous malformation and rehabilitation training may benefit the recovery of patients with CS caused by PE resulting from PAVM.
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http://dx.doi.org/10.1007/s40120-021-00275-yDOI Listing
September 2021

Effect of Yiqi Buxue Decoction on Hemodynamic Changes of the Uterine Artery and Fetal Umbilical Artery and Pregnancy Outcomes in Pregnant Patients with Pulmonary Arterial Hypertension.

Evid Based Complement Alternat Med 2021 17;2021:1849114. Epub 2021 Aug 17.

Department of Gynecology and Obstetrics, Beijing ChuiYangLiu Hospital, Beijing 100022, China.

Objective: To explore the effect of Yiqi Buxue decoction on hemodynamic changes of the uterine artery and fetal umbilical artery and pregnancy outcomes in pregnant patients with pulmonary arterial hypertension (PAH).

Methods: 120 pregnant patients with PAH treated in our hospital (January 2019-January 2020) were chosen as the research objects, and randomly split into group A ( = 60) and group B ( = 60). Both groups received routine treatment, and group B was treated with sildenafil citrate, while group A was treated with Yiqi Buxue decoction combined with sildenafil citrate. Both groups received 6 weeks of treatment to analyze the hemodynamic changes of the uterine artery and fetal umbilical artery and compare the cardiopulmonary function indexes and pregnancy outcomes between the two groups.

Results: The hemodynamic indexes of the uterine artery and fetal umbilical artery, cardiopulmonary function indexes, and pregnancy outcomes in group A after treatment were notably better compared with group B ( < 0.01).

Conclusion: Yiqi Buxue decoction can stabilize the hemodynamics of pregnant patients with PAH, improve their cardiopulmonary function, alleviate hypotension, and thus, reduce the possibility of adverse pregnancy outcomes, which should be popularized in practice.
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http://dx.doi.org/10.1155/2021/1849114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387161PMC
August 2021

A Novel Anti-Noise Fault Diagnosis Approach for Rolling Bearings Based on Convolutional Neural Network Fusing Frequency Domain Feature Matching Algorithm.

Sensors (Basel) 2021 Aug 17;21(16). Epub 2021 Aug 17.

Institute of Remote Sensing and Geographic Information System, Peking University, Beijing 100871, China.

The development of deep learning provides a new research method for fault diagnosis. However, in the industrial field, the labeled samples are insufficient and the noise interference is strong so that raw data obtained by the sensor are occupied with noise signal. It is difficult to recognize time-domain fault signals under the severe noise environment. In order to solve these problems, the convolutional neural network (CNN) fusing frequency domain feature matching algorithm (FDFM), called CNN-FDFM, is proposed in this paper. FDFM extracts key frequency features from signals in the frequency domain, which can maintain high accuracy in the case of strong noise and limited samples. CNN automatically extracts features from time-domain signals, and by using dropout to simulate noise input and increasing the size of the first-layer convolutional kernel, the anti-noise ability of the network is improved. Softmax with temperature parameter T and D-S evidence theory are used to fuse the two models. As FDFM and CNN can provide different diagnostic information in frequency domain, and time domain, respectively, the fused model CNN-FDFM achieves higher accuracy under severe noise environment. In the experiment, when a signal-to-noise ratio (SNR) drops to -10 dB, the diagnosis accuracy of CNN-FDFM still reaches 93.33%, higher than CNN's accuracy of 45.43%. Besides, when SNR is greater than -6 dB, the accuracy of CNN-FDFM is higher than 99%.
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http://dx.doi.org/10.3390/s21165532DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401700PMC
August 2021

Mental Health and Personality Traits during COVID-19 in China: A Latent Profile Analysis.

Int J Environ Res Public Health 2021 08 17;18(16). Epub 2021 Aug 17.

Psychology Department, Nottingham Trent University, Nottingham NG1 4FQ, UK.

During the COVID-19 pandemic, mental health problems have increased and are likely to be influenced by personality traits. The present study investigated the association between personality traits and mental health problems (anxiety, depression, post-traumatic stress syndrome (PTSD) symptoms, and obsessive-compulsive disorder (OCD) symptoms) through the person-centered approach because this has some advantages over the variable-centered approach. The data were collected from a sample of 765 Chinese citizens who participated in an online survey in October 2020. Latent profile analysis identified three latent personality profiles-highly adaptive, adaptive, and maladaptive. Highly adaptive individuals had higher extroversion, agreeableness, conscientiousness, openness, and lower neuroticism, while maladaptive individuals had lower extroversion, agreeableness, conscientiousness, openness, and higher neuroticism. Multivariate analysis of variance results showed that individuals with highly adaptive profiles had lower anxiety, depression, and PTSD symptoms compared to individuals with adaptive and maladaptive profiles. The findings of the present study indicate mental health professionals would benefit from formulated intervention plans given the association between latent personality profiles and mental health problems.
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http://dx.doi.org/10.3390/ijerph18168693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391495PMC
August 2021

Healthcare Warranty Policies Optimization for Chronic Diseases Based on Delay Time Concept.

Healthcare (Basel) 2021 Aug 23;9(8). Epub 2021 Aug 23.

School of Management, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

Warranties for healthcare can be greatly beneficial for cost reductions and improvements in patient satisfaction. Under healthcare warranties, healthcare providers receive a lump sum payment for the entire care episode, which covers a bundle of healthcare services, including treatment decisions during initial hospitalization and subsequent readmissions, as well as disease-monitoring plans composed of periodic follow-ups. Higher treatment intensities and more radical monitoring strategies result in higher medical costs, but high treatment intensities reduce the baseline readmission rates. This study intends to provide a systematic optimization framework for healthcare warranty policies. In this paper, the proposed model allows healthcare providers to determine the optimal combination of treatment decisions and disease-monitoring policies to minimize the total expected healthcare warranty cost over the prespecified period. Given the nature of the disease progression, we introduced a delay time model to simulate the progression of chronic diseases. Based on this, we formulated an accumulated age model to measure the effect of follow-up on the patient's readmission risk. By means of the proposed model, the optimal treatment intensity and the monitoring policy can be derived. A case study of pediatric type 1 diabetes mellitus is presented to illustrate the applicability of the proposed model. The findings could form the basis of developing effective healthcare warranty policies for patients with chronic diseases.
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http://dx.doi.org/10.3390/healthcare9081088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392548PMC
August 2021

Determination of Susceptibility Breakpoint for Cefquinome against in Pigs.

Antibiotics (Basel) 2021 Aug 9;10(8). Epub 2021 Aug 9.

National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, China.

(), a zoonotic pathogen, causes severe diseases in both pigs and human beings. Cefquinome can display excellent antibacterial activity against gram-negative and gram-positive bacteria. The aim of this study was to derive an optimal dosage of cefquinome against with a pharmacokinetic/pharmacodynamic (PK/PD) integration model in the target infection site and to investigate the cutoffs monitoring the changes of resistance. The minimum inhibitory concentration (MIC) distribution of cefquinome against 342 strains was determined. MIC and MIC were 0.06 and 0.25 μg/mL, respectively. The wild-type cutoff was calculated as 1 μg/mL. A two-compartmental model was applied to calculate the main pharmacokinetic parameters after 2 mg/kg cefquinome administered intramuscularly. An optimized dosage regimen of 3.08 mg/kg for 2-log CFU reduction was proposed by ex vivo PK/PD model of infected swine. The pharmacokinetic-pharmacodynamic cutoff was calculated as 0.06 μg/mL based on PK/PD targets. Based on the clinical effectiveness study of pathogenic MIC isolates, the clinical cutoff was calculated as 0.5 μg/mL. A clinical breakpoint was proposed as 1 μg/mL. In conclusion, the results offer a reference for determining susceptibility breakpoint of cefquinome against and avoiding resistance emergence by following the optimal dosage regimen.
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http://dx.doi.org/10.3390/antibiotics10080958DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389024PMC
August 2021

Application Evaluation of High-Flow Humidified Oxygen in Patients with Respiratory Failure after General Anesthesia Extubation for Multiple Injuries.

J Healthc Eng 2021 12;2021:1387129. Epub 2021 Aug 12.

Department of Emergency Medicine, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China.

Objective: To explore the effect of high-flow humidified oxygen therapy (HFNC) on patients with respiratory failure after general anesthesia extubation for multiple injuries.

Methods: 214 patients with multiple injuries in our hospital who underwent general anesthesia and suffered respiratory failure after weaning extubation and received sequential treatment were included. And, they were divided into control group (HFNC group) and observation group (NIMV group) according to the random number table method. Patients in the control group (125 cases) used high-flow nasal cannula (HFNC) after general anesthesia extubation, while patients in the observation group (89 cases) used NIMV. The respiratory rate, heart rate, finger pulse oxygen, oxygenation index (PaO/FiO), and re-tracheal intubation rate in the two groups were compared at 2, 8, and 24 hours after sequential treatment, and the mortality rate and hospital stay of ICU time were whole-course observation. And, the effect of conventional oxygen inhalation or HFNC on oxygenation and prognosis was analyzed. Then, SPSS21.0 software was applied for statistical analysis. To analyze the effect of conventional oxygen inhalation or HFNC on the improvement of oxygenation and prognosis, the receiver operating characteristic (ROC) curve can be used to evaluate the feasibility and treatment effect of high-flow nasal oxygen therapy (HFNC) for patients with respiratory failure after general anesthesia extubation for multiple injuries.

Results: Compared with the NIMV group, the respiratory frequency and heart rate of the HFNC group were significantly improved after 2 h, 8 h, and 24 h. At the same time, the finger pulse oxygen and oxygenation index increased significantly and returned to normal levels. HFNC can significantly reduce the reintubation rate, ICU hospital stay, and mortality rate. The area under the ROC curve was 0.9102, with 95% CI (0.8256, 0.9949) and < 0.0001.

Conclusion: For patients with multiple injuries undergoing general anesthesia and respiratory failure after weaning and extubation, the application of HFNC can moderate patients' heart rate and respiratory rate faster, increase oxygenation index and finger pulse oxygen, and reduce the reintubation rate, mortality rate, and ICU stay. At the same time, it can effectively improve the respiratory failure of patients after extubation and reduce the occurrence of complications.
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http://dx.doi.org/10.1155/2021/1387129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382541PMC
August 2021
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