Publications by authors named "Mei Gao-Takai"

2 Publications

  • Page 1 of 1

A low temperature promotes anthocyanin biosynthesis but does not accelerate endogenous abscisic acid accumulation in red-skinned grapes.

Plant Sci 2019 Jun 12;283:165-176. Epub 2019 Mar 12.

Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa 921-8836, Japan.

The effect of temperature on the concentrations of anthocyanins and endogenous plant hormones [abscisic acid (ABA), auxin, and cytokinin] were investigated using the detached berries of two related red-skinned cultivars cv. 'Aki Queen' and 'Ruby Roman' of the table grape Vitis labrusca L. × Vitis vinifera L. The total anthocyanin concentration of both cultivars was lower when exposed to high rather than low temperatures after véraison (the onset of ripening). However, the responses to temperature differed between the two cultivars, and anthocyanin accumulation could occur in 'Ruby Roman' at a higher temperature than in 'Aki Queen'. High temperatures increased the expression of VlMybA1-2 and VlMybA1-3, which encode myeloblastosis (MYB)-related transcription factors; however, the expression of the anthocyanin biosynthesis-related structural genes uridine diphosphate-d-glucose: flavonoid 3-O-glucosyltransferase, flavonoid 3'5' hydroxylase, and flavonoid O-methyltransferase at different temperatures did not correspond with that of the expression of MybAs. The concentration of ABA and its derivatives increased under high temperatures, but that of auxin and cytokinin decreased. The observation that high temperatures induced the accumulation of ABA and expression of VlMybA1s but not the expression of anthocyanin biosynthesis-related structural genes implied the operation of a mechanism different from up-regulation of anthocyanin synthesis by VlMybA1s in the temperature response of grape berries.
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June 2019

Overexpression of Prunus DAM6 inhibits growth, represses bud break competency of dormant buds and delays bud outgrowth in apple plants.

PLoS One 2019 9;14(4):e0214788. Epub 2019 Apr 9.

Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.

Most deciduous fruit trees cultivated in the temperate zone require a genotype-dependent amounts of chilling exposure for dormancy release and bud break. In Japanese apricot (Prunus mume), DORMANCY-ASSOCIATED MADS-box 6 (PmDAM6) may influence chilling-mediated dormancy release and bud break. In this study, we attempted to elucidate the biological functions of PmDAM6 related to dormancy regulation by analyzing PmDAM6-overexpressing transgenic apple (Malus spp.). We generated 35S:PmDAM6 lines and chemically inducible overexpression lines, 35S:PmDAM6-GR. In both overexpression lines, shoot growth was inhibited and early bud set was observed. In addition, PmDAM6 expression repressed bud break competency during dormancy and delayed bud break. Moreover, PmDAM6 expression increased abscisic acid levels and decreased cytokinins contents during the late dormancy and bud break stages in both 35S:PmDAM6 and 35S:PmDAM6-GR. Our analysis also suggested that abscisic acid levels increased during dormancy but subsequently decreased during dormancy release whereas cytokinins contents increased during the bud break stage in dormant Japanese apricot buds. We previously revealed that PmDAM6 expression is continuously down-regulated during dormancy release toward bud break in Japanese apricot. The PmDAM6 expression pattern was concurrent with a decrease and increase in the abscisic acid and cytokinins contents, respectively, in dormant Japanese apricot buds. Therefore, we hypothesize that PmDAM6 represses the bud break competency during dormancy and bud break stages in Japanese apricot by modulating abscisic acid and cytokinins accumulation in dormant buds.
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January 2020