Publications by authors named "Mehmet Tekin"

50 Publications

Phytochemical characterisation of Phlomis linearis Boiss. & Bal and screening for anticholinesterase, antiamylase, antimicrobial, and cytotoxic properties.

Turk J Chem 2021 28;45(2):387-399. Epub 2021 Apr 28.

Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskişehir Turkey.

In the present work, essential oil and fatty acids and extracts obtained from aerial parts of  Boiss. & Bal. were investigated for chemical composition and biological activities. The phytochemical analyses were conducted with gas chromatography-mass spectrometry/flame ionisation detector (GC-MS/FID) and liquid chromatography-mass spectromtetry (LC-MS/MS) techniques. The extracts and essential oil were studied for -amylase and acetylcholinesterase activities with two different spectrophotometric methods. Antimicrobial activities of the extracts were investigated by microdilution. The extracts were evaluated  for cytotoxic effects against cancer and normal cell lines by MTT assay. The essential oil (EO) contained -pinene (12.5%) and -caryophyllene (10.7%) as main compounds. Palmitic(26.5%) and nonadecanoic acids (26.6%) were determined as fatty acids. Phytochemical analysis of the extracts found phenolic acids, phlinosides, verbascoside, and flavonoids. The extracts and essential oil demonstrated poor -amylase inhibitory activity. The best acetylcholinesterase inhibitory activity was obtained for diethly ether extract of (67.2 ± 3.4%) at 10 mg /mL concentration. Ethyl acetate extract found to be effective against at a minimum inhibitory concentration (MIC) of 156.26 µg/mL. Diethyl ether extract of was active on A549 cell lines with an IC = 316 ± 4.16 µg/mL when compared with cisplatin IC = 24.43 ± 0.14 µg/mL. To the best of our knowledge, the present work is the first comprehensive report on anti-acetylcholinesterase, anti-α-amylase, and antimicrobial activities, as well as cytotoxic effects of .
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http://dx.doi.org/10.3906/kim-2009-59DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164195PMC
April 2021

A New Virulent Race of Wheat Stripe Rust Pathogen (Puccinia striiformis f. sp. tritici) on the Resistance Gene Yr5 in Turkey.

Plant Dis 2021 Apr 29. Epub 2021 Apr 29.

Akdeniz University, 37502, Field Crops, Akdeniz University Faculty of Agriculture Dept. of Field Crops, Antalya, Antalya, Konyaaltı, Turkey, 07058;

The gene Yr5 in wheat has generally been known to confer resistance to Puccinia striiformis f. sp. tritici races worldwide. However, a few reports indicated that some isolates of the stripe rust pathogen were virulent on the wheat genotypes with Yr5 in India, Australia, and China (Nagarajan 1986; Wellings and McIntosh 1990; Zhang et al. 2020). In April 2020, a stripe rust infection was observed on Triticum spelta 'Album' (TSA), the Yr5 donor, provided by Turkey Seed Gene Bank in a wheat breeding field at the experimental station of Akdeniz University in the Antalya province of Turkey. The sample was tested on the seedlings of the Yr single-gene lines in the Avocet S (AvS) background including Yr5 near-isogenic line (AvSYr5NIL), TSA, and susceptible bread wheat variety 'Morocco' according to the procedure reported by Wan et al. (2014), and found to be highly virulent on Morocco with infection type (IT) 9 and virulent on AvSYr5NIL and TSA with IT 7. To confirm this novel finding, single-pustule isolates derived from this sample were obtained from TSA and re-tested on the differentials and TSA. The Yr5-virulent isolate yielded similar infection types on these plants further verifying previous results. The single-pustule isolates were determined to be avirulent (Avr) (IT 0 to 6) on AvSYr1NIL, AvSYr8NIL, AvSYr9NIL, AvSYr10NIL, AvSYr32NIL, and AvSYr43NIL and virulent (Vr) (IT 7 to 9) on AvSYr5NIL, AvSYr6NIL, AvSYr7NIL, AvSYr17NIL, AvSYr24NIL, AvSYr27NIL, AvSYr44NIL, AvSYrSpNIL, and TSA. The isolates were identified as a new race and temporarily named TRVR20-5 based on its Avr/Vr pattern. Furthermore, to confirm the presence of Yr5, the genomic DNA of fresh leaves of AvSYr5NIL and TSA used in the virulence testing were isolated using NucleoSpin Plant II (Macherey-Nagel, Germany) according to the manufacturer's procedure. The genomic DNA samples were amplified with the primers STS-7 (5'-GTACAATTCACCTAGAGT-3') and STS-8 (5'-GCAAGTTTTCTCCCTATT-3') (Chen et al. 2003) using a thermal cycler (T-100, BioRad, USA), and the resulting PCR products were digested with DpnII and visualized on an agarose gel. AvSYr5NIL and TSA had the fragment linked to the resistance allele (308 bp) whereas AvS and Morocco had the fragment linked to the susceptible allele (181 bp). Thus, both wheat genotypes were confirmed to carry Yr5, and the new Pst race virulent to Yr5 was confirmed. To the best of our knowledge, this is the first report of a Pst race virulent to Yr5 in Turkey, where stripe rust epidemics have occured in 2 out of every 5 years with 1-5% crop losses in over 25% of the wheat growing areas (Chen 2020). In a nutshell, monitoring the Pst races virulent to Yr5 with a potential to cause devastating epidemics worldwide is crucial due to the fact that international wheat breeding programs have been using Yr5 to develop wheat cultivars with resistance to stripe rust.
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http://dx.doi.org/10.1094/PDIS-03-21-0629-PDNDOI Listing
April 2021

Ability of ESWL nomograms to predict stone-free rate in children.

J Pediatr Urol 2021 Mar 29. Epub 2021 Mar 29.

Baskent University Faculty of Medicine, Department of Urology, Ankara, Turkey.

Introduction: We aimed to evaluate whether the pediatric extracorporeal shock wave lithotripsy (ESWL) nomograms can predict stone-free status in children effectively and whether they are applicable to our series. We hypothesize that two current nomograms predicting successful treatment with ESWL in pediatric patients are valid.

Study Design: We evaluated 415 renal units (children <18 years) with eligible data who received ESWL treatment for upper urinary tract stones. Children's age, gender, stone size, stone surface area, stone location and history of previous intervention were recorded. Children with no residual fragments after ESWL treatment were designated as stone-free. The nomograms described by Dogan and Onal were implemented to our series for the prediction of stone-free status.

Results: Mean age of children was 64.7 ± 57.2 months. Male to female ratio was 219:196.78.8% (327) of children had single stone. Mean stone size was 10.0 ± 3.7 mm and mean stone surface area was 380.0 ± 72.2 mm. Our stone-free rate after single ESWL session was 52.5% (218/415). Mean residual stone size and stone surface area after single session was 6.4 ± 3.3 mm and 36.0 ± 44.2 mm respectively. There were no significant difference between stone-free children and children with residual fragments regarding gender, age and history of previous intervention. Mean stone size and stone surface area in stone-free children were lower and lower pole stones had the lowest stone-free rate (p < 0.05). Area under curve for Dogan and Onal nomogram were 0.628 and 0.580 respectively in ROC analysis (0.05). The agreement between Dogan and Onal score was moderate in our series. In multivariate analysis only stone surface area and Dogan score found to be independent predictors of stone-free status (p < 0.05).

Discussion: Only one study has assessed both nomograms in the literature. Both nomograms are reported to be independent predictors of stone free status. ROC analysis in our study revealed fair accuracy for both nomograms with higher area under curve for Dogan nomogram. Higher accuracy for both nomograms were reported by other authors. These nomograms offer practical data but more effective tools are needed to be developed for the prediction of stone-free status in pediatric ESWL.

Conclusions: Stone size and stone surface area are associated with stone clearance. Dogan and Onal nomograms can be useful in prediction of stone-free status in children. Dogan nomogram is superior to Onal nomogram.
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http://dx.doi.org/10.1016/j.jpurol.2021.03.025DOI Listing
March 2021

Comparison of Simultaneous Capillary Blood Gases and End-Tidal Carbon Dioxide in Mechanically Ventilated Pediatric Patients with Acute Respiratory Failure.

J Pediatr Intensive Care 2021 Mar 20;10(1):58-64. Epub 2020 Jul 20.

Department of Pediatrics, School of Medicine, Adiyaman University, Adiyaman, Turkey.

In the follow-up of ventilation, invasive blood gas analysis and noninvasive monitoring of end-tidal carbon dioxide (ETCO ) are used. We aimed to investigate the relationship between capillary partial pressure of carbon dioxide (PcCO ) levels and ETCO and also to investigate ETCO 's predictive feature of PcCO levels. This study included 28 female and 30 male pediatric patients; 28 patients were type-1 respiratory failure (RF), 16 patients were acute respiratory distress syndrome, and 14 patients were type-2 RF. Our results showed a significant correlation between ETCO and PcCO . Although the strength of the correlation was weak throughout the measurements, the strength of this correlation increased significantly in type-2 RF.
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http://dx.doi.org/10.1055/s-0040-1714096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870332PMC
March 2021

Assessment of (L.) J. Gay for phytochemical composition and antioxidant, anti-inflammatory, antidiabetic and antimicrobial activities.

Z Naturforsch C J Biosci 2021 Feb 9. Epub 2021 Feb 9.

Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskişehir, 26470, Turkey.

Phytochemical profiles of essential oil (), fatty acids, and -hexane (CAH), diethyl ether (CAD), ethyl acetate (CAE) and methanol extracts (CAM) of L. J. Gay (syn. L.) were investigated as well as their antioxidant, anti-inflammatory, antidiabetic and antimicrobial activites. The essential oil was characterized by the content of acetophenone (35.8%) and -caryophyllene (10.3%) by GC-MS/FID. Linoleic and oleic acid were found as main fatty acids. The major constituents of the extracts were found to be 5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, isorhamnetin glucoside, quercetin and quercetin glucoside by LC-MS/MS. Antioxidant activities of the extracts were determined by scavenging of DPPH and ABTS free radicals. Also, the inhibitory effects on lipoxygenase and -glucosidase enzymes were determined. Antimicrobial activity was evaluated against Gram positive, Gram negative bacteria and yeast pathogens. showed the highest antioxidant activity against DPPH and ABTS radicals with IC values of 126.60 and 144.40 μg/mL, respectively. In the anti-inflammatory activity, demonstrated the highest antilipoxygenase activity with an IC value of 105.40 μg/mL, whereas, howed the best inhibition of -glucosidase with an IC value of 396.40 μg/mL in the antidiabetic activity. was effective against at MIC = 312.5 µg/mL. This is the first report on antidiabetic, anti-inflammatory and antimicrobial activities of different extracts of .
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http://dx.doi.org/10.1515/znc-2020-0257DOI Listing
February 2021

The Comparison of LISA and INSURE techniques in term of neonatal morbidities and mortality among premature infants.

Acta Biomed 2020 11 10;91(4):e2020189. Epub 2020 Nov 10.

Department of Pediatrics, Baskent University Konya Training and Research Hospital, Konya, Turkey..

Background And Aim Of The Work: Respiratory distress syndrome (RDS) is the most common cause of respiratory failure among premature infants. The most important choice for the treatment of  RDS is still exogenous surfactant replacement therapy and respiratory support. Today, there are some different surfactant applying techniques. In this study, we aimed to evaluate the effects of the surfactant administration techniques in premature infants less than 33 weeks of gestational age.

Methods: The medical data were collected retrospectively from the medical records of Baskent University, Konya Training and Research Hospital between 2010 and 2016.  The patient divided into two subgroups as Less Invasive Surfactant Administration (LISA) group (n: 35) and Intubation- Surfactant administration and rapid Extubation (INSURE) group (n: 30). Two surfactant administration techniques were evaluated on the neonatal morbidities and mortality among premature infants.

Results: There were no significant differences in maternal and neonatal characteristics between the two groups. Duration on the nasal continues positive airway pressure (nCPAP) is significantly higher in the LISA group as compared with the INSURE group (p<0.001).  And also between two groups, there were no significant differences in term of neonatal mortality and morbidities.

Conclusion: The technique of the surfactant administration has no effect on the postnatal morbidities. LISA method is safe and effective as much as INSURE method, which is still a good alternative in centers with lack of experience about LISA. We need to perform studies that have larger sample size and prospective randomized controlled trials.
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http://dx.doi.org/10.23750/abm.v91i4.8845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927556PMC
November 2020

Clinical results of shock wave lithotripsy treatment in elderly patients with kidney stones: Results of 1433 patients.

Arch Ital Urol Androl 2020 Dec 18;92(4). Epub 2020 Dec 18.

Department of Urology, Baskent University, Adana.

Objective: In this study, it was aimed to evaluate the efficacy and safety of SWL treatment in elderly patients with kidney stones.

Materials And Methods: Data from a total of 3024 patients who underwent SWL treatment for urinary tract stone disease in three centers of our university were evaluated retrospectively. A total of 1433 patients in the adult age group treated for single kidney stones were included in the study. The patients were divided into 3 groups (18-40, 41-64 and ≥ 65) years depending on their age. Demographic data, stone parameters, stone-free rate (SFR) and clinically insignificant residual fragment (CIRF) rate, number of SWL sessions and complication rate were analyzed according to the age groups.

Results: The mean age of the patients was 47.38 ± 13.24 years. Stone size was significantly lower in the 18-40 years age group compared to other groups (p = 0.000) and the stones were mostly located on the right side in this age group (p = 0.007). There was no significant relationship between age groups and gender, stone localization, and number of SWL sessions. The overall SFR was 66.4%. Although the SFR was lower (61.4%) and the rate of multiple sessions (27.2%) was higher in ≥ 65 years group, there was no statistically significant difference between age groups regarding SFR, CIRF, need for additional sessions, and complication rates.

Conclusions: Due to its similar clinical results, treatment of SWL should not be ignored as a treatment option in the geriatric patient group with kidney stones.
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http://dx.doi.org/10.4081/aiua.2020.4.350DOI Listing
December 2020

Isolation and Identification of F Lavonol Glycosides from (Boiss. & Huet).

Iran J Pharm Res 2020 ;19(2):291-297

Ankara University, Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey.

In the last five decades study on plant secondary metabolites have been increasing. Higher plants with a wide range of secondary metabolites have been very important in the search of new therapeutic agents. In this study secondary metabolites of (Boiss. & Huet) which are endemic in Turkey, were studied. Flavonol glycosides (Rhamnocitrin-3--rhamninoside, Rhamnetin-3-O-rhamninoside, Rhamnazin- 3--rhamninoside, kaempferol3--rhamninoside and, kaempferol-3--glucosyl (1→2) rhamnoside) were isolated by different chromatographic methods and identified by H, C NMR, as well as 2D NMR and Mass spectroscopy techniques from ethyl acetate and aqueous fractions of 's methanolic extract. This is the first study about secondary metabolites of Turkish species.
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http://dx.doi.org/10.22037/ijpr.2019.111705.13360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667530PMC
January 2020

Atypical presentation in patients with 17 α-hydroxylase deficiency caused by a deletion in the CYP17A1 gene: short stature.

Turk J Pediatr 2020 ;62(5):851-857

Department of Medical Genetics, Düzce University Faculty of Medicine, Düzce, Turkey.

Background: Patients with 17α-hydroxylase deficiency (17 OHD) usually present with tall stature and eunuchoid features, rather than growth retardation. However, unlike the classic form of the disease, short stature due to a lack of pubertal growth spurt and sex hormone deficiency was present in our four cases. We wanted to emphasize that short stature might be the cause of first presentation in patients with 17 OHD.

Cases: We report five patients of Kurdish origin with 17 OHD, four of whom had short stature; two presented because of short stature and two were detected as having short stature. The external genitalia had a female appearance and was prepubertal in all cases. Hypertension was also detected in four of the patients. Serum biochemical and hormonal analyses were performed for each patient. Laboratory data suggesting severe growth hormone (GH) deficiency were obtained from one patient, while the other had a familial history suggesting constitutional delay of growth and puberty (CDGP). Whole exome sequence analysis of the CYP17A1 gene was performed on all patients. STR fragment analysis and multiplex ligation dependent probe amplification (MLPA) analysis was also performed to detect mutations associated with congenital adrenal hyperplasia (CAH) in the CYP17A1 gene. No mutation was detected in the whole exome sequence analysis of the CYP17A1 gene in all five patients, although wide deletions were identified in the 1st-6th exons of this gene at MLPA analysis.

Conclusions: Patients with 17α-hydroxylase deficiency can present with short stature because they have no pubertal growth spurt during adolescence. Therefore, 17 OHD should be considered in the differential diagnosis of patients with delayed puberty and short stature.
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http://dx.doi.org/10.24953/turkjped.2020.05.019DOI Listing
January 2020

Identification of Durum Wheat Cultivars and Their Tetraploid Relatives with Low Cadmium Content.

Food Technol Biotechnol 2020 Mar;58(1):49-56

Akdeniz University, Faculty of Agriculture, Department of Field Crops, 07059 Antalya, Turkey.

In this study, 71 durum wheat cultivars ( Desf.), 22 emmer wheat ( L.) and 11 wild emmer ( L.) genotypes were genetically characterized to determine the alleles associated with high cadmium (Cd) content. After genotypic characterization, 14 cultivars selected among all genotypes with low and high Cd content were phenotyped by a pot experiment to verify the genotypic data. Identification of 32 durum wheat, one emmer wheat and four wild emmer genotypes showed that they have alleles associated with high Cd content, while 68 genotypes of which 39 durum wheat, 21 emmer wheat and 7 wild emmer cultivars had alleles associated with low Cd content, respectively. Moreover, phenotypic data obtained from pot experiment were similar to the molecular data. To sum up, the marker successfully classified durum wheat cultivars into either high or low accumulators and these results can be safely used in breeding programs to improve new durum wheat cultivars with alleles associated with low Cd content. Due to routine use of phosphorus fertilizers in agricultural areas and other anthropogenic factors related to Cd toxicity, new durum wheat cultivars with low Cd content should be urgently developed for safe production of macaroni or other types of wheat products for human and animal consumption.
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http://dx.doi.org/10.17113/ftb.58.01.20.6531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365341PMC
March 2020

A novel mutation at the gene in a newborn with transient diabetes mellitus.

Turk Pediatri Ars 2020 19;55(2):195-198. Epub 2020 Jun 19.

Department of Pediatrics, Adıyaman University Faculty of Medicine, Adıyaman, Turkey.

Neonatal diabetes mellitus is a monogenic disease that can present with hyperglycemia, dehydration, failure to thrive, and ketoacidosis within the first six months of life. Neonatal diabetes mellitus can be transient or permanent. Here, we describe a 10-week-old infant with transient neonatal diabetes mellitus who presented with diabetic ketoacidosis and was found to have heterozygous a de novo mutation, p.Thr1381Asn in the ABCC8 gene, which encodes the SUR1 protein. There was no family history of diabetes mellitus and the parents were negative for the mutation at ABCC8. The patient started on insulin therapy and remission of diabetes occurred at 4 months of age. The patient remained euglycemic over a 2-year follow-up period without necessitating any medicine.
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http://dx.doi.org/10.14744/TurkPediatriArs.2019.01336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344130PMC
June 2020

Susceptibility Patterns of Multidrug-Resistant Acinetobacter baumannii.

Indian J Pediatr 2021 02 4;88(2):120-126. Epub 2020 Jun 4.

Department of Pediatrics, School of Medicine, Adiyaman University, Adiyaman, Turkey.

Objectives: To investigate the antimicrobial resistance patterns of multidrug-resistant Acinetobacter baumannii (MDRAB) in patients in pediatric intensive care units (PICU) in order to determine a guide for the empirical antibiotic treatment of MDRAB.

Methods: The authors retrospectively evaluated the medical records of patients with MDRAB infections in the PICU during a follow-up period, between January 2015 and January 2017. The identification of A. baumannii was performed using a BD Phoenix 100 Automated Microbiology System. A BD Phoenix NMIC/ID-400 commercial kit was used to test antibiotic susceptibility. All data was entered into Microsoft Excel, and the data was analyzed using SPSS version 23.0.

Results: The mean age of the patients was 8.1 ± 6.2 y. In all, 46 isolates were obtained from 33 patients. The most effective antimicrobial agents were colistin, trimethoprim/sulfamethoxazole, and tigecycline. Nevertheless, with the exception of colistin, no antibiotic was associated with a susceptibility rate of >45% for the isolates. Low sensitivities in 2015 to tigecycline, aminoglycosides, levofloxacin, and carbapenems had been lost in 2016.

Conclusions: Many drugs that were previously effective against MDRAB, have lost their effectiveness. Currently, there is no effective drug to fight MDRAB, apart from colistin. Thus, it is clear that new drugs and treatment protocols should be developed urgently.
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http://dx.doi.org/10.1007/s12098-020-03346-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271137PMC
February 2021

Antimicrobial and Anti-Inflammatory Activity of Some L. (Fabaceae) Species Growing in Turkey.

Turk J Pharm Sci 2019 Jun 27;16(2):240-245. Epub 2019 Mar 27.

Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey.

Objectives: The present study aimed to evaluate the antimicrobial and anti-inflammatory activities of methanol extracts and n-hexane, ethyl acetate, chloroform, and water fractions of five species, namely subsp. , and , growing in Turkey.

Materials And Methods: The antimicrobial activities were screened against ATCC 29213, ATCC 6633, ATCC 25922, ATCC 27853, and ATCC 10231. Broth dilution was used to determine the antimicrobial activities of extracts and fractions. anti-inflammatory activity of these extracts and fractions was determined using human red blood cell membrane stabilization.

Results: The results demonstrated that ethyl acetate fractions of the tested species exhibited higher antimicrobial activity than the other extracts. Among all of the tested extracts and fractions, the highest anti-inflammatory activity was detected in water fractions. Furthermore, water fractions of s showed better anti-inflammatory activity than acetylsalicylic acid and diclofenac sodium, which were used as standard drugs in this assay.

Conclusion: The results indicate the membrane stabilizing effect of the various extracts and fractions of the species and could constitute preliminary work for anti-inflammatory activity experiments.
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http://dx.doi.org/10.4274/tjps.galenos.2018.86719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227967PMC
June 2019

Comparison of cytotoxic effects of calcium silicate-based materials on human pulp fibroblasts Mehmet.

J Dent Res Dent Clin Dent Prospects 2019 ;13(4):241-246

Department of Biology and Genetics, Mustafa Kemal University, Hatay, Turkey.

This study aimed to compare the in vitro cytotoxicity of Theracal LC, BiodentineTM, iRoot BP Plus, and MTA Angelus on human pulp fibroblasts (HPF). Fifteen discs from each calcium silicate-based material were prepared in sterile Teflon molds. After setting, the fabricated discs were eluated with a culture medium for 24 h. HPF cells were plated onto 24-well plates at 5×10 cells/well, and the cells were exposed to the material eluates. The cell viability was evaluated with MTT assay at three different times (24, 48, and 72 h). Data were statistically analyzed. The apoptotic/necrotic status of HPF cells exposed to material eluates was determined by flow cytometry. The differences between the effects of Theracal LC, Biodentine, MTA Angelus, and iRoot BP Plus on HPF cells were found to be statistically significant (P<0.05). Theracal LC was found to be more cytotoxic considering other vital pulp capping materials at 24- (28.3%), 48- (44.9%), and 72-hour (49.2%) intervals. On the other hand, Biodentine showed the least cytotoxic effects (97.1%, 130.0%, and 103.7%, respectively) According to flow cytometry results, Theracal LC material increased apoptosis/necrosis ratios compared to the other materials. Based on the results of the present study, BiodentineTM, MTA Angelus, and iRoot BP Plus can be classified as biocompatible materials in vital endodontic treatments. However, the Theracal LC materials should be used carefully due to their cytotoxic effects.
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http://dx.doi.org/10.15171/joddd.2019.037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072094PMC
January 2019

Evaluation Using Spectral-Domain Optical Coherence Tomography of the Effects of Malnutrition on Ocular Parameters in Pediatric Patients.

Optom Vis Sci 2020 03;97(3):154-161

Department of Ophthalmology, School of Medicine, Adiyaman University, Adiyaman, Turkey.

Significance: We determined decreases in choroidal thickness and retinal nerve fiber layer (RNFL) thickness in all quadrants. Our findings show that the choroid and RNFL are affected before the emergence of ocular symptoms in malnourished children.

Purpose: We aimed to determine whether the RNFL, a component of the neuronal structure, and the choroid, supplying the retina, are affected in children with malnutrition using spectral-domain optical coherence tomography.

Methods: One hundred twenty-six malnourished patients without ocular symptoms, aged between 5 and 10 years, and 116 healthy children were included in the study. Age, sex, weight-for-age (WFA) z score, height-for-age z score, body mass index-for-age z score, and spectral-domain optical coherence tomography data were recorded.

Results: Average RNFL thickness was 96.5 μm (82.0 to 128.0 μm) in the malnutrition group and 111.0 μm (95.0 to 128.0 μm) in the control group (P < .001). Retinal nerve fiber layer thickness was statistically significantly lower in all quadrants in malnourished patients compared with the control group. Median choroidal thickness in the foveal center was 304.0 μm (250.0 to 375.0 μm) in the malnutrition group and 345.0 μm (280.0 to 403.0 μm) in the control group (P < .001). Choroidal thickness in all quadrants was also statistically significantly lower in malnourished patients. Positive correlation was determined between average RNFL thicknesses and WFA z score. Average RNFL thickness decreased as WFA z score decreased (r = 0.730 and P < .001). Positive correlation was also observed between choroidal thickness in the foveal center and WFA z score. Foveal center choroidal thickness decreased in line with WFA z score (r = 0.786 and P < .001).

Conclusions: Our results show that the retinal nerve fiber layer and choroidal thickness decreased in malnourished children without clinically reported ocular symptoms. A decreased retinal nerve fiber layer and choroidal thickness may be an important clue to the prevention of retinal pathologies that may develop at later ages if the malnutrition is not addressed.
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http://dx.doi.org/10.1097/OPX.0000000000001490DOI Listing
March 2020

Concurrence of Two Rare Conditions: Simultaneous Bilateral Spontaneous Pneumothorax and Congenital Pulmonary Airway Malformation.

J Pediatr Intensive Care 2019 Sep 25;8(3):178-180. Epub 2019 Jan 25.

Division of Pediatric Intensive Care Unit, School of Medicine, Adiyaman University, Adiyaman, Turkey.

Simultaneous bilateral spontaneous pneumothorax (SBSP) is a very rare type of pneumothorax. Most reported cases are associated with underlying lung diseases. In a small number of pediatric studies, SBSP was found to be associated with human bocavirus bronchiolitis, Langerhans cell histiocytosis, and infection. The present work examines an interesting case of type 2 congenital pulmonary airway malformation presenting with SBSP and bilateral multiple parenchymal cysts.
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http://dx.doi.org/10.1055/s-0039-1677814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6687480PMC
September 2019

The effect of luteolin in prevention of periodontal disease in Wistar rats.

J Periodontol 2019 12 1;90(12):1481-1489. Epub 2019 Jul 1.

Department of Periodontology, Faculty of Dentistry, Cumhuriyet University, Sivas, Turkey.

Background: Periodontal disease is the chronic infectious disease of the periodontium. Because of irreversibility, prevention of disease is one of the most important goals of periodontal treatment. The aim of this study was to evaluate the effect of luteolin, a powerful anti-inflammatory agent, on the prevention of experimental periodontitis by determining morphological and histological tissue alterations.

Methods: This study consisted of 28 rats and four experimental groups: healthy control group (C, n = 6); periodontitis group (P, n = 6); periodontitis and 50 mg/kg luteolin administered group (L-50, n = 8); and periodontitis and 100 mg/kg luteolin administered group (L-100, n = 8). Experimental periodontitis was induced via ligature method around lower right first molar teeth. All rats were euthanized 11 days after. The severity of periodontal destruction was determined by measuring alveolar bone loss under a stereomicroscope. Osteoblast and inflammatory cell counts were counted on hematoxylin-eosin-stained slides and osteoclasts were counted on tartrate-resistant acid phosphatase-stained slides. The levels of inducible nitric oxide synthase (iNOS), bone morphogenetic protein (BMP)-2, matrix metalloproteinase (MMP)-8, tissue inhibitor of MMP (TIMP)-1, receptor activator of nuclear factor κB ligand (RANKL), and osteoprotegerin (OPG) were determined by immunohistochemistry.

Results: The highest alveolar bone loss was observed in the periodontitis group and the luteolin administration decreased bone loss in both groups. Osteoblast cell number was higher and osteoclast and inflammatory cell numbers were lower in the P group compared to C, L-50, and L-100 groups. Luteolin, dose-dependently increased osteoblast cell counts. Luteolin attenuated periodontal inflammation in both L-50 and L-100 groups. Like osteoblast cell numbers, BMP-2 expressions were also elevated in luteolin groups. Both doses of luteolin significantly increased TIMP-1 and BMP-2 expressions and decreased MMP-8 levels. iNOS expressions increased in P group and L-100 significantly decreased iNOS levels. RANKL increased and OPG decreased in P group and 100 mg/kg luteolin increased OPG and decreased RANKL levels significantly.

Conclusions: Within the limits of present experimental study, luteolin successfully improved periodontal health in a ligature-induced experimental periodontitis model in Wistar rats. The decrease in inflammation, osteoclastic and collagenase activity and increase in osteoblastic activity are possibly involved in this process.
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http://dx.doi.org/10.1002/JPER.18-0584DOI Listing
December 2019

The effect of hyperlipidemia on bone graft regeneration of peri-implantal created defects in rabbits.

Int J Implant Dent 2019 May 15;5(1):18. Epub 2019 May 15.

Periodontology Department, Gulhane Faculty of Dentistry, University of Health Sciences, Ankara, Turkey.

Aim: It is reported that hyperlipidemia affects quality and density of bone and adversely affects wound healing. The effect of hyperlipidemia on implant osseointegration and peri-implant defect regeneration has not been fully explained. The purpose of this study was to examine the effects of hyperlipidemia on the healing potential of the materials used for peri-implant bone regeneration and implant stability.

Materials And Methods: Twelve male, New Zealand rabbits were used in this study. Half of the rabbits were fed a 2% cholesterol diet for 8 weeks to induce hypercholesterolemia. Peri-implant defects (7 mm diameter) were created in the tibias of rabbits and placed implants (3.3 mm in diameter). This study was conducted as a split-mouth design. Animals were randomly divided into two groups: (1) hypercholesterol+autogenous graft group and hypercholesterol+xenograft group (n = 6), and (2) autogenous graft and xenograft groups as controls (n = 6). At 8 weeks after surgery, the rabbits were euthanized. During implant surgery and at 8 weeks, implant stability was measured with resonance frequency analysis (RFA values). Bone-to-implant contact (BIC) was analyzed via histomorphometric analysis.

Results: Hyperlipidemic groups showed significantly lower BIC values than those of the control groups at 8 weeks (p < 0.05). According to baseline RFA readings, there was no significant difference between control and hyperlipidemic groups (p ˃ 0.05). The hypercholesterol+autogenous graft group had significantly lower RFA readings and BIC values than the hypercholesterol+xenograft group at 8 weeks (p < 0.05).

Conclusion: Within the limitations of this study, it was found that hyperlipidemia may negatively affect the implant stability especially in the autogenous group and also, may decrease peri-implant bone regeneration. However, further studies are necessary to confirm these results more.
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http://dx.doi.org/10.1186/s40729-019-0170-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517464PMC
May 2019

The effect of colchicine on alveolar bone loss in ligature-induced periodontitis.

Braz Oral Res 2019 Feb 11;33:e001. Epub 2019 Feb 11.

Gaziosmanpaşa University, School of Medicine,Department of Histology and Embryology, Tokat, Turkey.

Colchicine is widely used in the treatment of several inflammatory diseases due to its anti-inflammatory effect, but effects on bone metabolism are unclear. The aim of this study was to evaluate the effects of systemically-administered colchicine on healthy periodontium and experimentally-induced periodontitis. In total, 42 male Wistar rats were included in this study. A non-ligated group constituting the negative control group (Control, C, n = 6) and a ligature-only group forming the positive control group (LO, n = 12) were created separately. Twelve rats were treated with 0.4 mg/kg colchicine and another 12 with 1 mg/kg colchicine. In the colchicine-administered groups, right mandibles constituted the ligated groups (1 mgC-L or 0.4 mgC-L) and left mandibles formed the corresponding non-ligated controls (1mgC or 0.4mgC). Silk ligatures were placed at the gingival margin of the lower first molars. The animals were euthanized at different time-points of healing (11 or 30 days). Alveolar bone loss was clinically measured and TRAP+ osteoclasts, osteoblastic activity, and MMP-1 expression were examined histologically. There was no increase in alveolar bone loss with either colchicine dose in healthy periodontium (p > 0.05) and the highest level of alveolar bone loss, TRAP+ osteoclast number, and MMP-1 expression were measured in the LO group (p < 0.05). The 0.4 mgC-L group showed less alveolar bone loss at 11 days (p < 0.05), but greater loss at 30 days. The 1 mgC-L group showed higher osteoblast number than the other ligated groups (p < 0.05) at both time-points. In summary, colchicine did not increase alveolar bone loss in healthy periodontium and also may tend to reduce periodontitis progression. However, further extensive study is necessary to understand the mechanism of colchicine action on alveolar bone loss in periodontitis.
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http://dx.doi.org/10.1590/1807-3107bor-2019.vol33.0001DOI Listing
February 2019

Thrombocytopenia in Infants: CMV-Related ITP or CMV-Related Thrombocytopenia?

Indian J Hematol Blood Transfus 2018 Oct 6;34(4):774-775. Epub 2018 Mar 6.

Department of Pediatrics, School of Medicine, Adiyaman University, Kahta Street, 02000 Adiyaman, Turkey.

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http://dx.doi.org/10.1007/s12288-018-0945-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186251PMC
October 2018

Anti-inflammatory and antibacterial evaluation of Thymus sipyleus Boiss. subsp. sipyleus var. sipyleus essential oil against rhinosinusitis pathogens.

Microb Pathog 2018 Sep 19;122:117-121. Epub 2018 Jun 19.

Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey.

Thymus sipyleus Boiss. subsp. sipyleus var. sipyleus of the Lamiaceae, locally known as thyme scented lemon, which is an endemic taxon collected from Sivas in Anatolia, was investigated in this study due to its folk medicine use against rhinosinusitis. The aromatic characteristics of the plant material gave the idea for the detailed evaluation of the volatiles and essential oil thereof. Consequently, the oil was obtained by Clevenger type hydrodistillation followed by gas chromatography-flame ionization detector (GC-FID) and gas chromatography/mass spectrometry (GC/MS) analyses for phytochemical characterization. To confirm the folk medicinal use against sinusitis, in vitro antimicrobial activities of the essential oil was evaluated by agar diffusion, microdilution and vapour diffusion methods against selected rhinosinusitis associated strains such as Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), S. epidermidis, Streptococcus pyogenes, S. pneumoniae, Pseudomonas aeruginosa, Haemophilus influenzae and Moraxella catarrhalis. Additionally, the in vitro anti-inflammatory activity was evaluated by 5-lipoxygenase (5-LOX) inhibitory effect of the essential oil spectrophotometrically. Furthermore, the composition of the volatiles of the vapour phase of the oil was determined by headspace-solid phase microextraction (HS-SPME-GC/MS) after 15 min and 24 h in accordance with antimicrobial vapour diffusion method conditions, respectively. According to the analytical results, the main component was determined as thymol (66.2%). Whereas in the HS-SPME method p-cymene (26.1%) and γ-terpinene (26%) were identified as the main volatile components within the 15. min., and thymol (75.3%) after 24 h, respectively. The antibacterial activity against rhinosinusitis pathogens varied between 160 and 1250 μg/mL minimum inhibitory concentrations, with the best inhibitory effects observed against the S. aureus, S. pyogenes and M. catarrhalis. The anti-inflammatory activity of the oil was determined as 12.1 ± 1.8% in 100 μg/mL. The results showed the in vitro antimicrobial and anti-inflammatory potential of the oil also in vapour phase against sinusitis supporting the traditional use.
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http://dx.doi.org/10.1016/j.micpath.2018.06.025DOI Listing
September 2018

activity assessment of some species used as traditional wound healer along with identification of the phytochemical profile by a new validated HPLC method.

Iran J Basic Med Sci 2018 Feb;21(2):145-152

Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330, Ankara, Turkey.

Objectives: species are traditionally used as insecticide, and externally wound healer as well as for anti-inflammatory and antihistaminic properties. The wound-healing and anti-inflammatory potential of four species, (Lam.) Willd. subsp. (TA) (Bornm.) Grierson (TH), (Lab.) Schultz Bip. subsp. (TD), and L. (TV) was investigated.

Materials And Methods: The chloroform (CHCl) and methanol:water (80:20) extracts were prepared from the aerial parts of each plant. For assessment of the wound-healing activity, linear incision on rats and circular excision on mice wound models were used and histopathological analyses were conducted on the tissues treated with the test materials. For the evaluation of the anti-inflammatory activity, Whittle Method based on the inhibition of the acetic acid-induced increase in capillary permeability was used. In order to elucidate the phytochemical contents of the extracts, HPLC profiles of active fractions were screened and quantitative analysis was conducted within the scope of HPLC analysis.

Results: The CHCl extracts of TD, TA and TV were found to have significant wound healing activity (37.1%, 30.8% and 26.1% tensile strength; 88.05%, 72.93% and 44.88% contraction values, respectively) and anti-inflammatory activities (31.5% and 26.6% inhibition values for TD and TA). Parthenolide content of the CHCl extracts of TA, TH and TV were found 242.66±1.53, 190.16±5.62 and 177.51±3.73 µg/100 mg plant material, respectively.

Conclusion: According to the results, the other secondary metabolites present in the aerial parts of the species possibly exerted synergistic effects on the observed healing of the wounds.
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http://dx.doi.org/10.22038/IJBMS.2018.24258.6055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811753PMC
February 2018

Molecular and agro-morphological characterization of ancient wheat landraces of turkey.

BMC Plant Biol 2017 Nov 14;17(Suppl 1):171. Epub 2017 Nov 14.

Department of Field Crops, Faculty of Agriculture, Akdeniz University, Antalya, Turkey.

Background: Turkey is one of the important gene centers for many crop species. In this research, some ancient wheats such as tetraploid and diploid hulled wheats together with hexaploid tir wheats (Triticum aestivum ssp. leucospermum Korn.) landraces mainly adapted to harsh winter conditions of Eastern Anatolian region of Turkey were characterized at agro-morphological and molecular level. Totally 50 hulled wheat population from Kastamonu, Konya and Kayseri provinces and 15 tir wheats from Kars provinces of Turkey were in-situ collected for characterization in 2013. Some quantitative and qualitative traits of each population were determined.

Results: Twenty three hulled wheat population collected from Kastamonu province were distinguished into nine emmer and 14 einkorn wheats at morphological level. Additionally, Konya, Kayseri and Kars population were characterized as einkorn, emmer and tir wheat, respectively. Among the evaluated traits, protein ratios of hulled wheats were strikingly higher than registered cultivars. All the populations were also examined by molecular level by using fluorescently labelled 11 polymorphic SSRs primers. The primers exhibited 104 bands, ranging from 6 to 16 with a mean value 9.45 per loci. The clustering analysis separated the germplasm into two clusters which were also divided into two subclusters based on genetic similarity coefficient. Sixty-five population and five checks were analyzed to estimate mean number of alleles (N), expected and observed heterozygoties (He and Ho), polymorphism information content (PIC), Wright fix index (F), genetic deviation from Hardy-Weinberg expectation (Fit-Fis) and genetic variation (Fst) were determined as 9.45, 0.71, 0.07, 0.67, 0.90, 0.39, 0.87 and 0.39, respectively. A clear genetic deviation from Hardy - Weinberg expectation was observed among population in particular. These results showed considerable genetic variation among landraces rather than within population.

Conclusions: These molecular information has revealed genetically diverse einkorn, emmer wheat and tir wheat population could be used as parents for further breeding studies in both Turkey and abroad. Furthermore, the molecular analysis has also generally discriminated the germplasm into ploidy level.
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http://dx.doi.org/10.1186/s12870-017-1133-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5688393PMC
November 2017

The First Infant Death Associated With Human Coronavirus NL63 Infection.

Pediatr Infect Dis J 2017 02;36(2):231-233

From the *Department of Pediatrics, Adiyaman University, School of Medicine, Adiyaman, Turkey; and †Public Health Institutions of Turkey, National Virology Reference Laboratory/National Influenza Center, Ankara, Turkey.

Human coronavirus NL63 (HCoV-NL63) primarily infects the upper respiratory tract. However, it may cause severe lower respiratory tract infection, and the clinical course may be severe in immunocompromised patients. To our knowledge, child death due to HCoV-NL63 has not been reported. We present a fatal lower respiratory tract disease associated with HCoV-NL63 in a 7-month-old malnourished infant.
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http://dx.doi.org/10.1097/INF.0000000000001390DOI Listing
February 2017

Evaluation of hepatoprotective and antidiabetic activity of Alchemilla mollis.

Biomed Pharmacother 2017 Feb 12;86:172-176. Epub 2016 Dec 12.

Ankara University, Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey.

Alchemilla mollis (Buser) Rothm aerial part and root methanolic-water extracts were evaluated for their hepatoprotective activity on carbon tetrachloride induced hepatotoxicity and hypoglycemic activity on alloxan-induced diabetic mice. None of the tested extracts exhibited effects on blood glucose levels. However hepatoprotective activity results have revealed that serum ALT levels were significantly lowered by both the aerial part and root extracts at doses of 100mg/kg and 200mg/kg. Histopathological examination showed that A. mollis aerial parts and roots induced significant recovery from cellular damage; when compared to the carbon tetrachloride group, the most significant activity was observed with A. mollis aerial part extracts at a dose of 200mg/kg. There is evidence of a hepatoprotective activity of A. mollis on the phenolic content of the plant, especially in the case of flavonoids, which have potent antioxidant properties.
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http://dx.doi.org/10.1016/j.biopha.2016.12.005DOI Listing
February 2017

Evaluation of Choroidal Thickness in Children With Iron Deficiency Anemia.

Invest Ophthalmol Vis Sci 2016 Nov;57(14):5940-5944

Department of Pediatrics, Adiyaman University School of Medicine, Adiyaman, Turkey.

Purpose: The purpose of this study was to determine whether there are differences in choroidal thickness in children with iron deficiency anemia (IDA).

Methods: Fifty-two patients with IDA and 54 healthy children between 3 and 16 years of age were enrolled in this study. After complete eye examinations were conducted for each participant, the choroidal thickness was measured using optical coherence tomography. Correlations between the choroidal thickness and clinical and laboratory parameters were also evaluated.

Results: There were no statistically significant differences between the two groups in terms of visual acuity, intraocular pressure, central corneal thickness, or axial length (P > 0.05). The choroidal thicknesses at the foveal center were 303.13 ± 27.14 μm in the IDA patients and 333.67 ± 39.77 μm in the healthy control children (P < 0.001); additionally, the choroidal thicknesses at each point within the horizontal nasal and temporal quadrants were thinner in the IDA group. There were positive correlations between the choroidal thickness and hemoglobin (r = 0.337; P < 0.001), mean corpuscular volume (r = 0.305; P = 0.001), iron (r = 0.264; P = 0.006), and ferritin (r = 0.287; P = 0.003) levels; however, there were no correlations between the clinical or ocular characteristics and the choroidal thickness.

Conclusions: The patients with IDA had significantly thinner choroidal thicknesses than those of the healthy children. Choroidal thinning in childhood may be an early sign of deterioration in the ocular blood circulation, without any risk of atherosclerosis in advanced age in the patients with IDA.
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http://dx.doi.org/10.1167/iovs.15-18713DOI Listing
November 2016

The Combined Use of Inhaled and Intravenous Steroids for Children With Chemical Pneumonitis After Ingestion of Paint Thinner.

Iran Red Crescent Med J 2016 Jul 15;18(7):e24300. Epub 2016 May 15.

Medical Faculty, Pediatric Department, Adiyaman University, Adiyaman, Turkey.

Background: Some studies in the literature support the use of either inhaled or systemic steroids for the treatment of chemical pneumonitis; however, no data have been published on the combined use of inhaled and intravenous (IV) steroids.

Objectives: This brief report describes the effective use of inhaled plus systemic steroids in managing six critical pediatric patients.

Patients And Methods: Medical records of patients were analyzed retrospectively.

Results: Of the six patients, 83.3% (n = 5) were male and 16.7% (n = 1) were female, with a mean age of 2.1 ± 0.49 years. The most common clinical signs were dyspnea (83.3%), fever (66.6%), and vomiting (66.6%). Owing to supportive treatments and the combined steroid treatment, respiratory distress diminished and there was no need for oxygen in any of the patients after 5 days. All patients were discharged without any sequelae.

Conclusions: The use of steroids in treating hydrocarbon pneumonias is still controversial. However, we suggest that the combined use of inhaled and intravenous steroids had positive effects on the clinical and radiological recovery of our patients.
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http://dx.doi.org/10.5812/ircmj.24300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5020603PMC
July 2016

Antibacterial resistance patterns of pediatric community-acquired urinary infection: Overview.

Pediatr Int 2017 Mar 6;59(3):309-315. Epub 2016 Nov 6.

Department of Pediatrics, School of Medicine, Adiyaman University, Adiyaman, Turkey.

Background: Urinary tract infection (UTI) is common in children. The aim of this study was therefor to construct a guide for the empirical antibiotic treatment of community-acquired UTI by investigating the etiology and antimicrobial resistance patterns of uropathogens and analyzing the epidemiological and clinical patient characteristics.

Methods: A total of 158 children with positive urine culture were included in the study. Antibiotic susceptibility testing was performed with Vitek 2 Compact for 28 commonly used antimicrobials.

Results: Mean age was 3.36 ± 3.38 years (range, 45 days-15 years). Escherichia coli (60.1%), and Klebsiella spp. (16.5%) were the most common uropathogens. For all Gram-negative isolates, a high level of resistance was found against ampicillin/sulbactam (60.1%), trimethoprim/sulfamethoxazole (44.2%), cefazolin (36.2%), cefuroxime sodium (33.5%), and amoxicillin/clavulanate (31.5%). A low level of resistance was noted against cefepime (8.7%), ertapenem (4.6%), norfloxacin (1.3%), and meropenem (0.7%). There was no resistance against amikacin.

Conclusions: There is high antibiotic resistance in children with UTI. The patterns of uropathogen antimicrobial resistance vary in susceptibility to antimicrobials depending on region and time. Thus, the trends of antibiotic susceptibility patterns should be analyzed periodically to select the appropriate regimen for UTI treatment.
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http://dx.doi.org/10.1111/ped.13139DOI Listing
March 2017

Author's reply to the letter to the editor regarding "Hemoglobin level is a more predictive diagnostic marker for acute pyelonephritis than mean platelet volume".

Clin Exp Nephrol 2016 10 26;20(5):825. Epub 2016 Apr 26.

Department of Pediatrics, Adiyaman University, School of Medicine, Kahta Street, 02000, Adiyaman, Turkey.

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http://dx.doi.org/10.1007/s10157-016-1269-5DOI Listing
October 2016

Choroidal Thickness in Children with Beta Thalassemia Major.

Optom Vis Sci 2016 06;93(6):600-6

*MD Departments of Ophthalmology (AS, SB, ASK) and Pediatrics (MT, CK, HA), School of Medicine, Adiyaman University, Adiyaman, Turkey.

Purpose: The purpose of this study was to determine whether there are differences in choroidal thickness in children with beta thalassemia major (β-TM).

Methods: Thirty-five patients with β-TM and 38 healthy children aged between 3 and 16 years participated in the study. After complete eye examinations were conducted on the participants, choroidal thickness measurements were performed using optical coherence tomography. Correlations between choroidal thickness and laboratory and clinical parameters, such as age, sex, hemoglobin and ferritin levels, duration of disease, type and duration of chelating therapy, visual acuity, intraocular pressure, central corneal thickness, and axial length were also evaluated.

Results: The mean ages for the study group and for the control group were 8.2 ± 2.7 and 7.9 ± 2.4 years, respectively. There were no statistical differences between groups in terms of visual acuity, intraocular pressure, central corneal thickness, or axial length (p > 0.05). Choroidal thicknesses at the foveal center were 286 ± 33 μm in β-TM patients and 335 ± 423 μm in the healthy control children. Choroidal thicknesses at each point within the horizontal nasal and temporal quadrants were thinner in the β-TM group. There was a positive correlation between choroidal thickness and hemoglobin levels and a negative correlation between choroidal thickness and ferritin levels (r = 0.924, p < 0.001 and r = -0.947, p < 0.001, respectively). There was no correlation between clinical or ocular characteristics and choroidal thickness.

Conclusions: Choroidal thickness was significantly thinner in all quadrants in children with β-TM. This thinning of the choroid may be the reason for the development of eye disorders in older patients with β-TM.
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http://dx.doi.org/10.1097/OPX.0000000000000833DOI Listing
June 2016