Publications by authors named "Mehmet Kaya"

412 Publications

Prevalence of Anemia and Associated Risk Factors among Pregnant Women, What is the Role of Antenatal Care in Prevention? A Cross-sectional Study.

J Coll Physicians Surg Pak 2021 Nov;31(11):1341-1345

Department of Internal Medicine, Firat University Faculty of Medicine, Elazig, Turkey.

Objective: To determine the prevalence of anemia with the associated factors in pregnant women and the effect of family medicine practice and antenatal care services provided by family physicians on the prevention of anemia during pregnancy.

Study Design: Community-based cross-sectional study.

Place And Duration Of Study: Family Health Centres in the Elazig region, Turkey from January to June 2019.

Methodology: A total of 495 pregnant women, attending the family health centres, were included. Women were administered a questionnaire related with the anemia and related factors; and hemoglobin values were drawn. Hemoglobin concentration <11 g/dl was classified as anaemic. Sociodemographic, individual dietary diversity and obstetric characteristics of the participants were obtained from structured questionnaire form. Binary logistic regression model was employed to determine the effect of explanatory variables on dependent variable anemia.   Results: The prevalence of anemia during pregnancy was 27.9% (Hb<11.0 gr/dl). Prevalence of anemia was found lower in participants with higher education levels (p=0.005) and having nutrition education (p=0.004). Income status (OR=0.017 [95%Cl:0.002-0.15, p<0.001]), dark tea preference (OR=87.127 [95%Cl:17.68-429.36, p<0.001]), and iron supplementation time (OR=0.945 [95%Cl: 0.91-0.98, p=0.002]) were determined as the independent predictors of anemia in pregnancy.

Conclusion: Low income, insufficient iron supplementation treatment and dark tea preference were the main predictors of anemia. Most of the factors that cause anemia during pregnancy can be prevented with patient education and supportive treatments. Therefore, the authors recommend applying effective and quality prenatal care and patient education programs to reduce anemia during pregnancy. Key Words: Pregnant women, Anemia, Prenatal care, Family practice, Turkey.
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http://dx.doi.org/10.29271/jcpsp.2021.11.1341DOI Listing
November 2021

The Effects of Secondary Hyperparathyroidism on Circadian Blood Pressure in Hemodialysis Patients.

J Coll Physicians Surg Pak 2021 Nov;31(11):1325-1330

Department of Internal Medicine, Division of Nephrology, Tokat City Hospital, Tokat,Turkey.

Objective: To determine the relationship between secondary hyperparathyroidism (sHPT) and circadian blood pressure (BP) rthym among patients undergoing hemodialysis treatment.

Study Design: A descriptive and prospective cohort study.

Place And Duration Of Study:   Department of Nephrology, Firat University Hospital, Turkey, between June and December 2019.

Methodology: Eighty-five patients, undergoing hemodialysis three times a week, were included. The BP parameters and circadian blood pressure rhythm were measured by the mobil-o-graph sphygmomanometer. Non-dipper blood pressure phenomenon was defined as a night-time mean artery pressure (MAP)  decrease of <10% from the daytime MAP; sHPT was defined as parathormone >300 pg/ml.

Results: Of the 85 participants, mean age was 57.07 ± 14.46 years. In cohort, sHPT rate 58.8% (n =50) and non-dipper blood pressure pattern rate was 64.7% (n = 55). Systolic blood pressure (p=0.001), diastolic blood pressure (p=0.001), and mean arterial blood pressure (p<0.001) were higher in participants with sHPT. sHPT (p = 0.003) was an independent risk factor for non-dipper blood pressure pattern (odds ratio [OR] 0.065, 95% CI: 0.11-0.390). A negative correlation was identified between parathormone and the reduction in night blood pressure (r = -0.346, p=0.001).

Conclusion: Secondary hyperparathyroidism can cause non-dipper blood pressure pattern by reducing the reduction in night blood pressure. Treatment of sHPT is important in the control of blood pressure and normal circadian blood pressure rhythm in tertiary protection in hemodialysis patients. Key Words: Blood pressure, Circadian rhythm, Hyperparathyroidism secondary, Hemodialysis, Chronic kidney diseases.
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http://dx.doi.org/10.29271/jcpsp.2021.11.1325DOI Listing
November 2021

HER2-low breast cancer could be associated with an increased risk of brain metastasis.

Int J Clin Oncol 2021 Oct 18. Epub 2021 Oct 18.

Department of Medical Oncology, Hacettepe University Cancer Institute, Hacettepe University Oncology Hospital, 06100, Sıhhıye, Ankara, Turkey.

Purpose: The HER2-low breast cancer is a newly recognized entity with the clinical characteristics is yet to be defined. We hypothesized that HER2-low breast cancer could lead to an increased rate of brain metastases in patients with localized breast cancer. We tested this hypothesis in a large cohort of breast cancer patients with long follow-up.

Methods: We included 2686 adult breast cancer patients followed up in Hacettepe University Cancer Center. Patients with 1 + positive HER2 expression and 2 + HER2 expression with a negative FISH were categorized as HER2-low disease. We evaluated the brain metastasis risk with binary logistic regression analyses and reported odds ratios (OR) with 95% confidence intervals (CI).

Results: During a median 95.4 (IQR 72.6-123.1) month follow-up, 184 patients developed brain metastasis (6.9%). The brain metastases were developed in 5.1% of the patients with HER2-negative disease, 8.5% of the patients with HER2-low disease, and 10.1% of the patients with HER2-positive disease. A multivariable binary logistic regression model demonstrated an increased risk of brain metastasis in patients with HER2-low disease (OR: 1.611, 95% CI 1.055-2.460, p = 0.027) and in HER2-positive patients (OR: 1.837, 95% CI 1.308-2.580, p < 0.001). Additionally, HR + -HER2-low disease was associated with a decreased DFS compared to HR + -HER2-negative disease (p = 0.008).

Conclusion: In this study, we observed an increased risk of brain metastasis in localized breast cancer patients with HER2-low disease. We think that a high level of vigilance and a low threshold for brain imaging could benefit HER2-low breast cancer patients similar to the patients with HER-positive disease.
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http://dx.doi.org/10.1007/s10147-021-02049-wDOI Listing
October 2021

The role of vitamin D deficiency on the Covid-19: A systematic review and meta-analysis of observational studies.

Epidemiol Health 2021 Sep 23:e2021074. Epub 2021 Sep 23.

Firat University, Elazig, Turkey.

Objectives: Although vaccination has started, it seems that Covid-19 will continue to threaten public health for a long time. Therefore, in addition to the vaccine, the use of supplements to support the immune system may also be important. The main purpose of this study is to indicate the possible effect of low serum vitamin D (25(OH)D<20 ng/mL or 50nmol/L) on the Covid-19 infection and outcomes.

Methods: To accomplish our objectives, we searched on Google Scholar, PubMed, Scopus, Web of Science, and ScienceDirect databases without any language restrictions for articles between 01.01.2020 and 15.12.2020. We performed three meta-analyses to combine the OR values by paying attention to laboratory measurement units for vitamin D and the measured serum 25(OH)D level.

Results: 23 eligible studies involving the relationship between vitamin D and Covid-19 infection/outcomes were identified (n=206861). We applied three meta-analyses called D-CIMA, D-CSMA and D-CMMA for Covid-19 infection, severity, and mortality, respectively. According to obtained result from D-CIMA, one which has low serum vitamin D are 1.64 times (95%CI=[1.32-2.04],p<0.001) more likely to get Covid-19 infection. In D-CSMA, we found that people with the serum 25(OH)D level below 20ng/mL or 50nmol/L have 2.58 times (95%CI=[1.28-5.19],p=0.008) more likely to risk having severe Covid-19. We obtained from D-CMMA that low vitamin D level has no effect on Covid-19 mortality (OR=2.42 95%CI=[0.73-8.04],p=0.148).

Conclusion: Vitamin D deficiency increases the risk of Covid-19 infection and the potential for the severity of the disease. Therefore, vitamin D supplements should be added to prevention and treatment protocols for Covid-19 disease.
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http://dx.doi.org/10.4178/epih.e2021074DOI Listing
September 2021

Stress Analysis Based on Simultaneous Heart Rate Variability and EEG Monitoring.

IEEE J Transl Eng Health Med 2021 23;9:2700607. Epub 2021 Aug 23.

Department of Biomedical and Chemical Engineering and SciencesFlorida Institute of Technology Melbourne FL 32901 USA.

Objective: Stress is a significant risk factor for various diseases such as hypertension, heart attack, stroke, and even sudden death. Stress can also lead to psychological and behavioral disorders. Heart rate variability (HRV) can reflect changes in stress levels while other physiological factors, like blood pressure, are within acceptable ranges. Electroencephalogram (EEG) is a vital technique for studying brain activities and provides useful data regarding changes in mental status. This study incorporates EEG and a detailed HRV analysis to have a better understanding and analysis of stress. Investigating the correlation between EEG and HRV under stress conditions is valuable since they provide complementary information regarding stress.

Methods: Simultaneous electrocardiogram (ECG) and EEG recordings were obtained from fifteen subjects. HRV /EEG features were analyzed and compared in rest, stress, and meditation conditions. A one-way ANOVA and correlation coefficient were used for statistical analysis to explore the correlation between HRV features and features extracted from EEG.

Results: The HRV features LF (low frequency), HF (high frequency), LF/HF, and rMSSD (root mean square of the successive differences) correlated with EEG features, including alpha power band in the left hemisphere and alpha band power asymmetry.

Conclusion: This study demonstrated five significant relationships between EEG and HRV features associated with stress. The ability to use stress-related EEG features in combination with correlated HRV features could help improve detecting stress and monitoring the progress of stress treatments/therapies. The outcomes of this study could enhance the efficiency of stress management technologies such as meditation studies and bio-feedback training.
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http://dx.doi.org/10.1109/JTEHM.2021.3106803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407658PMC
August 2021

Letter to editor "vitamin D deficiency aggravates covid-19: systematic review and meta-analysis".

Crit Rev Food Sci Nutr 2021 Aug 12:1-2. Epub 2021 Aug 12.

Department of Biostatistics and Medical Informatics, School of Medicine, Fırat University, Elazığ, Turkey.

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http://dx.doi.org/10.1080/10408398.2021.1951650DOI Listing
August 2021

Lancefield classification and antimicrobial resistance of hemolytic streptococci isolated from bovine mastitis.

Vet Ital 2021 May 11;57(1):41-47. Epub 2021 May 11.

Department of Microbiology, Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Turkey.

Streptococcal species are known to be responsible for bovine mastitis. The aim of the present study was to determine antimicrobial drug resistance patterns of hemolytic streptococci distributed according to Lancefield serogrouping. Streptococcus sp. strains were isolated from 124 bovine milk samples from 31 cows with subclinical or clinical mastitis submitted to Mehmet Akif Ersoy University Faculty of Veterinary Medicine, Department of Microbiology Laboratory in Burdur province, Turkey from January 2015 to January 2017. A total of 63 Streptococcus sp. were isolated and the most frequently obtained isolates were classified as Lancefield's serogroup B (84.13%), the remaining isolates as serogroup F (15.87%). Out of 63 isolates, 53 (84.13%) showed beta‑hemolytic activity whereas 10 (15.87%) alpha‑hemolytic activity. Antimicrobial resistance was assessed by disk diffusion test against the most common antibiotics used in the field. Among the 63 Streptococcus sp. tested, the highest antimicrobial resistance patterns were observed for neomycin (95.24%), trimethoprim sulphamethoxazole (87.30%) and gentamicin (69.84%). None of the isolates showed resistance to amoxicillin‑clavulanic acid, except for one serogroup F isolate. The resistance rates for the other antimicrobials ranged from 1.59% to 38.04%. A total of 50 isolates exibited multi‑drug resistance to ≥ 3 antimicrobial agents tested. Overall, our results suggested that there is an urgent need to enhance awareness among the dairy farmers in choosing the appropriate drug for treating mastitis.
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http://dx.doi.org/10.12834/VetIt.1855.9879.3DOI Listing
May 2021

Exosomal delivery of therapeutic modulators through the blood-brain barrier; promise and pitfalls.

Cell Biosci 2021 Jul 22;11(1):142. Epub 2021 Jul 22.

Koç University Research Center for Translational Medicine (KUTTAM), Rumeli Feneri, 34450, Sariyer, Istanbul, Turkey.

Nowadays, a large population around the world, especially the elderly, suffers from neurological inflammatory and degenerative disorders/diseases. Current drug delivery strategies are facing different challenges because of the presence of the BBB, which limits the transport of various substances and cells to brain parenchyma. Additionally, the low rate of successful cell transplantation to the brain injury sites leads to efforts to find alternative therapies. Stem cell byproducts such as exosomes are touted as natural nano-drug carriers with 50-100 nm in diameter. These nano-sized particles could harbor and transfer a plethora of therapeutic agents and biological cargos to the brain. These nanoparticles would offer a solution to maintain paracrine cell-to-cell communications under healthy and inflammatory conditions. The main question is that the existence of the intact BBB could limit exosomal trafficking. Does BBB possess some molecular mechanisms that facilitate the exosomal delivery compared to the circulating cell? Although preliminary studies have shown that exosomes could cross the BBB, the exact molecular mechanism(s) beyond this phenomenon remains unclear. In this review, we tried to compile some facts about exosome delivery through the BBB and propose some mechanisms that regulate exosomal cross in pathological and physiological conditions.
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http://dx.doi.org/10.1186/s13578-021-00650-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296716PMC
July 2021

Evaluation of serum/salivary levels of carnosine and cotinine in recurrent wheezing of young children with passive smoking.

Turk J Pediatr 2021 ;63(3):404-416

Departments of Biostatistics, Fırat University Faculty of Medicine, Elazığ, Turkey.

Background: Recurrent wheezing is common in young children, with a cumulative prevalence of up to 40 % in the first 6 years of life. In this study, we aimed to evaluate the relationship between the number of wheezing episodes and the number of cigarettes smoked at home and serum / saliva cotinine and carnosine levels in children with recurrent wheezing.

Methods: This study was conducted with 80 young children with recurrent wheezing, aged between 1-4 years and 50 healthy control groups. Patient population was divided into three groups depending on the number of their exposure to cigarette smoke and wheezing attacks. Serum cotinine, saliva cotinine, serum carnosine, saliva carnosine, vitamin D levels were measured by using the ELISA method.

Results: A significant relationship for serum cotinine and saliva cotinine levels was found between groups (p < 0.05). It was determined that as the number of exposure to cigarette smoke and number of wheezing episodes in young children with recurrent wheezing increased, the level of serum/saliva cotinine levels increased significantly, compared to the control group. In contrast, it was determined that as the number of exposure to cigarette smoke and number of wheezing episodes in young children with recurrent wheezing increased, serum/saliva carnosine levels decreased significantly, compared to the control group. In addition, a significant difference in serum vitamin D levels was found between healthy young children and young children with recurrent wheezing (p < 0.05).

Conclusions: We think that the measurement of salivary cotinine is a useful and noninvasive marker to evaluate passive smoking exposure in the etiology of recurrent wheezing in young children.
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http://dx.doi.org/10.24953/turkjped.2021.03.007DOI Listing
August 2021

Characterization of Suspicious Microcalcifications on Mammography Using 2D Shear-Wave Elastography.

Ultrasound Med Biol 2021 09 11;47(9):2532-2542. Epub 2021 Jun 11.

Department of Pathology, Gaziosmanspasa Education and Research Hospital, Gaziosmanpasa, Istanbul, Turkey.

Our aim was to investigate the correlations between the findings of two-dimensional shear-wave elastography (2D-SWE) and the histopathologic results of microcalcifications (MCs) visualized using ultrasonography (USG). Fifty people with suspicious MCs without accompanying mass were evaluated. They underwent USG and 2D-SWE before USG-guided tru-cut biopsy. SWE values and histopathologic features were compared statistically. The variables between groups were analyzed using the Mann-Whitney U test. Receiver operating characteristic analysis was performed and cut-off values determined to discriminate malignancy, invasiveness and high grade. Pathology confirmed 27 malignant lesions (18 invasive ductal carcinomas, one invasive lobular and eight ductal carcinomas in situ) and 23 benign ones. There was a statistically significant difference between the SWE values of malignant and benign MCs (p < 0.001). The diagnostic performance of SWE for malignancy, invasiveness and high grade were as follows, repectively: sensitivity (93%, 83%, 88%), specificity (91%, 88%, 53%), positive predictive value (93%, 94%, 44%), negative predictive value (91%, 70%, 90%) and area under the curve (0.952, 0.885, 0.776). Cut-off values were determined as 57 kPa for malignancy, 124 kPa for invasiveness and 124.5 kPa for high grade. In conclusion, SWE is a useful method in clinical practice for characterizing MCs that can be visualized with USG.
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http://dx.doi.org/10.1016/j.ultrasmedbio.2021.05.008DOI Listing
September 2021

The role of hemogram parameters and C-reactive protein in predicting mortality in COVID-19 infection.

Int J Clin Pract 2021 Jul 30;75(7):e14256. Epub 2021 Apr 30.

Department of Emergency Medicine, Faculty of Medicine, Mugla Sitki Kocman University, Mugla, Turkey.

Aim: This study aimed to investigate hemogram parameters and C-reactive protein (CRP) that can be used in clinical practice to predict mortality in hospitalized patients with a diagnosis of COVID-19.

Methods: This cohort study was conducted at University Hospital, which is a designated hospital for COVID-19 patients. Adult patients who were admitted to our hospital emergency department with suspected COVID-19 and who were hospitalized in our institution with a COVID-19 diagnosis were analysed.

Results: There were 148 patients hospitalized with COVID-19. All-cause mortality of follow-up was 12.8%. There were statistically significant results between the two groups (survivors and nonsurvivors), which were classified based on hospital mortality rates, in terms of the lymphocyte to C-reactive protein ratio (LCRP), systemic immune inflammation index (SII), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), CRP concentration and comorbid disease. In a receiver operating characteristic (ROC), curve analysis, LCRP, NLR, PLR and SII area under the curve (AUC) for in-hospital mortality were 0.817, 0.816, 0.733 and 0.742, respectively. Based on an LCRP value of 1 for in-hospital mortality, the sensitivity and specificity rates were 100% and 86.8%, respectively. Based on the average SII of 2699 for in-hospital mortality, the sensitivity, specificity and accuracy rates were 68.4%, 77.5% and 76.3%, respectively. A total of 19 patients died during hospitalization. All of these patients had an LCRP level ≤ 1; 14 had an NLR level ≤ 10.8; 13 had an SII ≥ 2699 (Fisher's exact test, P = .000). Independent predictors of in-hospital mortality rates were LCRP < 1, PLR, SII ≥ 2699, white blood cell count, CRP, age, comorbidities, and ICU stay.

Conclusions: We concluded that inflammatory parameters, such as LRCP, SII and NLR, were associated with disease severity and could be used as potentially important risk factors for COVID-19 progression.
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http://dx.doi.org/10.1111/ijcp.14256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250321PMC
July 2021

The effectiveness of two different exercise approaches in adolescent idiopathic scoliosis: A single-blind, randomized-controlled trial.

PLoS One 2021 15;16(4):e0249492. Epub 2021 Apr 15.

School of Physical Therapy and Rehabilitation, Ahi Evran University, Kırşehir, Turkey.

Objectives: The purpose of this study was to compare the efficacy of two different types of exercise methods in patients with adolescent idiopathic scoliosis.

Methods: In total, 28 subjects with adolescent idiopathic scoliosis with a mild curve magnitude (10°-26°) were randomly divided into two groups: the Schroth group (n = 14) and the core group (n = 14). The patients in the Schroth group were treated with supervised Schroth exercises, and the patients in the core group were treated with supervised core stabilization exercises; both groups performed the exercises for three days per week for a total of 10 weeks, and both were given additional traditional exercises to perform. Assessment included Cobb angle (Radiography), trunk rotation (Adam's test), cosmetic trunk deformity (Walter Reed Visual Assessment Scale), spinal mobility (Spinal Mouse), peripheral muscle strength (Biodex System 4-Pro), and quality of life (Scoliosis Research Society-22 questionnaire).

Results: It was found that patients in the Schroth group showed greater improvement in Cobb angles, thoracic trunk rotation angle, cosmetic trunk deformity, spinal mobility, and quality of life than those in the core group (p<0.05), except for in lumbar trunk rotation angle. Peripheral muscle strength improvement was greater in the core group than in the Schroth group (p<0.05).

Conclusion: Schroth exercises are more effective than core stabilization exercises in the correction of scoliosis and related problems in mild adolescent idiopathic scoliosis, and core stabilization exercises are more effective than Schroth exercises in the improvement of peripheral muscle strength.

Trial Registration: NCT04421157.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249492PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049223PMC
September 2021

Tissue distribution and cellular localization of gold nanocarriers with bound oligonucleotides.

Nanomedicine (Lond) 2021 04 14;16(9):709-720. Epub 2021 Apr 14.

Department of Life, Health & Chemical Sciences, The Open University, Milton Keynes, UK.

The aim of the study was to determine how the addition of a DNA oligonucleotide cargo to 3-nm gold glyconanoparticles would affect tissue distribution. Gold glyconanoparticles with 1-6 covalently bound oligonucleotides (40 nt dsDNA) were injected into rats and allowed to circulate for 10 min. Organs were harvested and gold quantitated by inductively coupled plasma mass spectrometry. Cellular localization of the nanocarriers was determined by electron microscopy. Addition of DNA cargo to the nanocarriers prevented localization in the kidney but increased localization in liver hepatocytes and splenic macrophages. There was no significant change in heart, lung or brain. DNA increases the size and adds a strong negative charge to the nanoparticles, which radically affects tissue distribution.
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http://dx.doi.org/10.2217/nnm-2020-0469DOI Listing
April 2021

Arterial Stiffness and its Related Factors in Patients on Hemodialysis.

J Coll Physicians Surg Pak 2021 Feb;31(2):138-143

Department of Biostatistics and Medical Informatics, Faculty of Medicine, Firat University, Turkey.

Objective: To evaluate the prevalence of arterial stiffness in patients on hemodialysis and related factors that aggravate the condition.

Study Design: Prospective-cohort study.

Place And Duration Of Study:   Department of Nephrology, Firat University Hospital, Turkey, from August to October 2019.

Methodology: Central blood pressure (BP), augmentation index (AIx) and aortic pulse wave velocity score (PWVs) were assessed using the mobil-o-graph pulse wave velocity system.  Increased arterial stiffness (AS) wgnas defined by the aortic PWV of  >10 m/s.

Results: The mean PVWs were 8.14 ± 2.39 m/s the mean AIx was 21.23 ± 11.58 m/s, and AS was 33.7% in hemodialysis patients. Systolic blood pressure, pulse rate, pulse pressure, central systolic blood pressure, older age, HD vintage, HT vintage, creatinine, CRP and neutrophil leukocyte ratio (NLR), were related factors with AS. Multivariate linear regression analysis showed that systolic blood pressure (β=0.322; 95% CI: 0.007-0.042; p = 0.006), and age of patients (β=0.787; 95% CI: 0.095-0.127; p<0.001) were independent determinants of PWVs.

Conclusion: The frequency of AS was 33.7% in hemodialysis patients without a history of cardiovascular disease. Increased age and systolic blood pressure were independent factors affecting PWV. Effective blood pressure control therapy can reduce AS in hemodialysis patients. Key Words: Pulse wave velocity, Arterial stiffness, Cardiovasculer disease, Systolic blood pressure, Hemodialysis.
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http://dx.doi.org/10.29271/jcpsp.2021.02.138DOI Listing
February 2021

Effect of humate supplementation to feed and/or litter on performance, intestinal viscosity, litter quality, and occurrence of footpad dermatitis in broilers fed barley-based diets.

Trop Anim Health Prod 2021 Jan 7;53(1):80. Epub 2021 Jan 7.

Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Aydın Adnan Menderes University, Işıklı, Efeler, 09016, Aydın, Turkey.

We investigated the effect of in-feed and/or in-litter supplemental humate against footpad dermatitis (FPD) in broilers fed diets based on barley. Three hundred and sixty 1-day-old Ross 308 broiler chickens were randomly distributed to 24 floor pens (4 treatments, each consisting of 6 replicate pens; 15 chickens per pen) as a completely randomized design with 2 × 2 factorial arrangement of two levels of supplemental humate in feed (0 and 1 g/kg feed) and litter (0 and 5 g/kg litter). Growth performance, intestinal viscosity, litter quality, and incidence and severity of FPD in broilers were measured. In addition, malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined in blood and footpad tissues of broilers with different FPD scores. The results revealed that there was no interaction between humate supplementation to feed and litter. Neither dietary nor litter supplementation of humate had a significant effect on growth performance, intestinal viscosity, litter quality, and occurrence of FPD. And also, MDA and SOD levels in serum and footpad tissue did not affect by either dietary or litter supplementation of humate. The presence of FPD (score 1) had no effect on MDA and SOD levels in serum, however, increased the MDA and SOD levels (P < 0.001, P = 0.001, respectively) in footpad tissue of broilers. The intestinal viscosity did not differ between FPD scores 0 and 1. In conclusion, findings of this experiment suggest that humate supplementation to feed and litter did not alleviate FPD development in broilers fed diets based on barley. In addition, the presence of FPD lesions increases the MDA and SOD levels in the footpad tissues.
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http://dx.doi.org/10.1007/s11250-020-02530-0DOI Listing
January 2021

Identifying side effects of commonly used drugs in the treatment of Covid 19.

Sci Rep 2020 12 9;10(1):21508. Epub 2020 Dec 9.

Department of Computer Science, University of Calgary, Calgary, AB, Canada.

To increase the success in Covid 19 treatment, many drug suggestions are presented, and some clinical studies are shared in the literature. There have been some attempts to use some of these drugs in combination. However, using more than one drug together may cause serious side effects on patients. Therefore, detecting drug-drug interactions of the drugs used will be of great importance in the treatment of Covid 19. In this study, the interactions of 8 drugs used for Covid 19 treatment with 645 different drugs and possible side effects estimates have been produced using Graph Convolutional Networks. As a result of the experiments, it has been found that the hematopoietic system and the cardiovascular system are exposed to more side effects than other organs. Among the focused drugs, Heparin and Atazanavir appear to cause more adverse reactions than other drugs. In addition, as it is known that some of these 8 drugs are used together in Covid-19 treatment, the side effects caused by using these drugs together are shared. With the experimental results obtained, it is aimed to facilitate the selection of the drugs and increase the success of Covid 19 treatment according to the targeted patient.
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http://dx.doi.org/10.1038/s41598-020-78697-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7725770PMC
December 2020

Changes of HMGB-1 and sTLR4 levels in cerebrospinal fluid of patients with febrile seizures.

Epilepsy Res 2021 01 28;169:106516. Epub 2020 Nov 28.

Department of Pediatric Neurology, Faculty of Medicine, Akdeniz University, Antalya, Turkey. Electronic address:

Purpose: Fever-induced inflammatory processes and pro-inflammatory cytokines have gained importance in recent years in the pathogenesis of febrile convulsion. Increased levels of HMGB1 (high mobility group box 1), one of the most important pro-inflammatory cytokines, are associated with prolongation of seizure duration, recurrence of seizures and the development of epilepsy. Changes in the sTLR4 level (soluble toll-like receptor 4) in the cerebrospinal fluid (CSF) are thought to be associated with memory and learning functions. In our study, we aimed to evaluate changes in HMGB1 and sTLR4 levels in patients who had febrile seizures between 6 months and 6 years.

Methods: Forty patients who were admitted to Akdeniz University Medical Faculty Hospital between April 2016 and April 2018 with a complaint of febrile seizure and 45 patients whose CSF samples were taken for complaints other than febrile convulsion (control group) were included in our study.

Results: Comparison of the CSF HMGB1 levels of the febrile convulsion group and control group revealed a statistically significant increase in patients with febrile convulsions (p: 0.001). Comparison of the subgroups revealed that the mean value of CSF HMGB1 level was highest in the complex FS group with a mean value of 3363.9 ± 835,47 pg/mL. Comparison of the patient and control groups revealed that the changes in CSF sTLR4 levels were not statistically significant.

Conclusion: HMGB1 level, a key inflammatory molecule, was significantly higher in the CSF of children with febrile seizures. Our data suggest that the HMGB1 network may contribute to the generation of febrile seizures in children.
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http://dx.doi.org/10.1016/j.eplepsyres.2020.106516DOI Listing
January 2021

Basic physiology of the blood-brain barrier in health and disease: a brief overview.

Tissue Barriers 2021 01 15;9(1):1840913. Epub 2020 Nov 15.

Koç University School of Medicine Department of Histology and Embryology, Koç University Research Center for Translational Medicine , Istanbul, Turkey.

The blood-brain barrier (BBB), a dynamic interface between blood and brain constituted mainly by endothelial cells of brain microvessels, robustly restricts the entry of potentially harmful blood-sourced substances and cells into the brain, however, many therapeutically active agents concurrently cannot gain access into the brain at effective doses in the presence of an intact barrier. On the other hand, breakdown of BBB integrity may involve in the pathogenesis of various neurodegenerative diseases. Besides, certain diseases/disorders such as Alzheimer's disease, hypertension, and epilepsy are associated with varying degrees of BBB disruption. In this review, we aim to highlight the current knowledge on the cellular and molecular composition of the BBB with special emphasis on the major transport pathways across the barrier type endothelial cells. We further provide a discussion on the innovative brain drug delivery strategies in which the obstacle formed by BBB interferes with effective pharmacological treatment of neurodegenerative diseases/disorders.
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http://dx.doi.org/10.1080/21688370.2020.1840913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849738PMC
January 2021

The role of fine needle aspiration biopsy in deep lobe parotid tumors: Comparison of preoperative cytology and postoperative histopathologic results.

Am J Otolaryngol 2021 Jan - Feb;42(1):102590. Epub 2020 Jun 10.

Sisli Hamidiye Etfal Training and Research Hospital, Department of Ear Nose Throat Head and Neck Surgery, Istanbul, Turkey.

Purpose: Major salivary gland tumors constitute almost 3% of head and neck tumors. Tumors located exclusively in the deep lobe are not common and 20% of parotid gland tumors originate from deep lobe under the branches of the facial nerve. Accuracy of fine needle aspiration biopsy (FNAB) procedure in salivary gland tumors have been studied extensively, however there isn't any data regarding usefulness of FNAB in tumors located exclusively in deep lobe of parotid gland. In this study we aimed to assess the use of FNAB in deep lobe parotid tumors.

Materials And Methods: We retrospectively analyzed 51 patients with deep lobe parotid tumors who underwent surgery in our clinic between January 2013-December 2018. Characteristics of patients were recorded. Preoperative FNAB results and postoperative final histopathologic diagnosis were compared. Statistical analysis was performed using SPSS 24.0 was used for statistical analysis.

Results: The number of patients that met the inclusion criteria was 51. The mean age of patients were 49.2(14-86). In 40 (78.4) of the patients, tumor was reported as benign and in 11 (21.6) patients FNAB diagnosis was suspicious for malignancy, malignant or non-diagnostic. In final histopathologic diagnosis, 42 of the tumors were benign and 9 were malignant. The most common benign tumor type was pleomorphic adenoma which constitutes 27 of the cases (52.9%). Regarding detection of malignant disease, the sensitivity of FNAB was 90.4%, specificity was 77.7%, positive predictive value was 95%, negative predictive value was 63.6%. There was a substantial agreement between FNAB and final histopathologic diagnosis(kappa = 0,628).

Conclusions: FNAB is a safe and reliable tool to evaluate deep lobe parotid tumors. It is an important part of preoperative surgical planning and can help the surgeon in patient counseling. FNAB with ultrasound guidance is recommended for deep lobe tumors.
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http://dx.doi.org/10.1016/j.amjoto.2020.102590DOI Listing
April 2021

Fabrication, characterization, and in vivo biocompatibility evaluation of titanium-niobium implants.

Proc Inst Mech Eng H 2021 Jan 28;235(1):99-108. Epub 2020 Sep 28.

Meterial Science and Engineering, Engineering Faculty, Gebze Technical University, Gebze, Turkey.

In this study, biocompatible titanium-niobium (Ti-Nb) alloys were fabricated by using powder metallurgy methods. Physical, morphological, thermal, and mechanical analyses were performed and their in vivo compatibility was evaluated. Besides α, β, and α″ martensitic phases, α+β Widmanstätten phase due to increasing sintering temperature was seen in the microstructure of the alloys. Phase transformation temperatures of the samples decreased as Nb content increased. The ratio of Nb in the samples affected their mechanical properties. No toxic effect was observed on implanted sites. This study shows that Ti-Nb alloys can be potentially used for orthopedic applications without any toxic effects.
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http://dx.doi.org/10.1177/0954411920960854DOI Listing
January 2021

The effect of swimming training on adrenomedullin levels, oxidative stress variables, and gastrocnemius muscle contractile properties in hypertensive rats.

Clin Exp Hypertens 2021 Feb 27;43(2):131-137. Epub 2020 Sep 27.

Department of Physiology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University , Aydin, Turkey.

 Regular exercise may have beneficial effects on high blood-pressure, as shown in different types of experimental hypertension models in rats. The present study aims to investigate the effects of 6-week swimming training on blood pressure, oxidative stress variables of selected tissues, serum adrenomedullin (ADM) levels, and in situ muscle contraction in rats with hypertension induced by Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME), an inhibitor of endothelial nitric oxide synthases (eNOs).  Twenty-six male Sprague Dawley, 8 weeks of age, rats were randomly divided into four groups: (I) normotensive (C), (II) normotensive + exercise (E), (III) hypertensive (L), and (IV) hypertensive + exercise (LE). Hypertension was induced by the oral administration of L-NAME (60 mg/kg) for 6 weeks. Exercise was performed 5 times (1-h each) per week for 6 weeks. At the end of the experiment, blood and tissue samples (the gastrocnemius muscle, heart, kidney, and thoracic aorta) were collected following contractile properties of the gastrocnemius muscle  weredetermined. In the collected tissues, oxidative stress (e.g., lipid oxidation and antioxidant enzyme activity) and serum ADM levels were measured. 6-week L-NAME administration  (Group L) led to a significant increase in systolic and diastolic blood pressure compared to other groups.   Importantly, 6-week exercise caused a protective effect of high blood pressure in the rats received L-NAME (Group LE). The level of ADM was lower in the rats received L-NAME than that of the control group. L-NAME increased lipid peroxidation in the thoracic aorta and decreased superoxide dismutase in the heart, kidney and muscle, and decreased catalase and glutathione in the heart. However, the exercise intervention did not have protective effect on the L-NAME-mediated oxidative damage in the collected tissues.   In conclusion, 6-week exercise intervention rescued rats from high blood pressure, but did not have ameliorative effect on the decreased ADM levels.
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http://dx.doi.org/10.1080/10641963.2020.1825726DOI Listing
February 2021

Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.

Methods Mol Biol 2021 ;2367:87-103

Department of Physiology, Koç University School of Medicine, Istanbul, Turkey.

The blood-brain barrier (BBB) constituted by endothelial cells of brain microvessels is a dynamic interface, which controls and regulates the transport of various substances including peptides, proteins, ions, vitamins, hormones, and immune cells from the circulation into the brain parenchyma. Certain diseases/disorders such as Alzheimer's disease, sepsis, and hypertension can lead to varying degrees of BBB disruption. Moreover, impairment of BBB integrity has been implicated in the pathogenesis of various neurodegenerative diseases like epilepsy. In attempts to explore the wide spectrum of pathophysiologic mechanisms of these diseases/disorders, a variety of experimental insults targeted to the BBB integrity in vitro in cell culture models and in vivo in laboratory animals have been shown to alter BBB permeability causing enhanced transport of certain tracers such as sodium fluorescein, cadaverine-Alexa fluor, horseradish peroxidase, FITC-dextran, albumin-Alexa fluor conjugates, and Evans blue dye across the barrier. The permeability changes in barrier-type endothelial cells can be assessed by intravascular infusion of exogenous tracers and subsequent detection of the extravasated tracer in the brain tissue, which enable functional and structural analysis of BBB integrity. In this chapter, we aimed to highlight the current knowledge on the use of four most commonly performed tracers, namely, Evans blue, sodium fluorescein, albumin-Alexa fluor conjugates, and horseradish peroxidase. The experimental methodologies that we use in our laboratory for the detection of these tracers by macroscopy, spectrophotometry, spectrophotofluorometry, confocal laser scanning microscopy, and electron microscopy are also discussed. Tracing studies at the morphological level are mainly aimed at the identification of the tracers both in the barrier-related cells and brain parenchyma. In addition, BBB permeability to the tracers can be quantified using spectrophotometric and spectrophotofluorometric assays and image analysis by confocal laser scanning microscopy and electron microscopy. The results of our studies conducted under various experimental settings using the mentioned tracers indicate that barrier-type endothelial cells in brain microvessels orchestrate the paracellular and/or transcellular trafficking of substances across BBB. These efforts may not only contribute to designing approaches for the management of diseases/disorders associated with BBB breakdown but may also provide new insights for developing novel brain drug delivery strategies.
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http://dx.doi.org/10.1007/7651_2020_316DOI Listing
January 2021

Targeted delivery of lacosamide-conjugated gold nanoparticles into the brain in temporal lobe epilepsy in rats.

Life Sci 2020 Sep 11;257:118081. Epub 2020 Jul 11.

Department of Physiology, School of Medicine, Koç University, Istanbul, Turkey. Electronic address:

Temporal lobe epilepsy (TLE) is the most common form of epilepsy with focal seizures, and currently available drugs may fail to provide a thorough treatment of the patients. The present study demonstrates the utility of glucose-coated gold nanoparticles (GNPs) as selective carriers of an antiepileptic drug, lacosamide (LCM), in developing a strategy to cross the blood-brain barrier to overcome drug resistance. Intravenous administration of LCM-loaded GNPs to epileptic animals yielded significantly higher nanoparticle levels in the hippocampus compared to the nanoparticle administration to intact animals. The amplitude and frequency of EEG-waves in both ictal and interictal stages decreased significantly after LCM-GNP administration to animals with TLE, while a decrease in the number of seizures was also observed though statistically insignificant. In these animals, malondialdehyde was unaffected, and glutathione levels were lower in the hippocampus compared to sham. Ultrastructurally, LCM-GNPs were observed in the brain parenchyma after intravenous injection to animals with TLE. We conclude that glucose-coated GNPs can be efficient in transferring effective doses of LCM into the brain enabling elimination of the need to administer high doses of the drug, and hence, may represent a new approach in the treatment of drug-resistant TLE.
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http://dx.doi.org/10.1016/j.lfs.2020.118081DOI Listing
September 2020

The effects of CLP-induced sepsis on proliferation and apoptosis of granulosa and theca cells in rat ovary: A histochemical and ultrastructural study.

Reprod Biol 2020 Sep 20;20(3):408-416. Epub 2020 May 20.

Istanbul University, Istanbul Faculty of Medicine, Department of Histology and Embryology, Istanbul 34093, Turkey.

Sepsis is defined as a systemic inflammatory response to infection. This study is aimed to evaluate the effects of experimental sepsis on the proliferation and apoptosis of granulosa and theca cells in the rat ovary. 28-day-old immature Wistar-Albino female rats were treated with pregnant mare serum gonadotrophin to develop the first generation of preovulatory follicles. Sepsis was induced by cecal ligation and puncture (CLP). Following in vivo 5-Bromo-2-deoxyuridine (BrdU) labeling, animals were sacrificed and ovaries were embedded in paraffin and Epon. Besides electron microscopic evaluation, BrdU, cleaved caspase-3, p27 immunostaining, and TUNEL labeling were performed. In CLP-operated animals, cleaved caspase-3 immunoreactivity was significantly increased in Graafian follicles. TUNEL and BrdU labeling in the ovarian follicles were not statistically different between CLP and sham-operated rats. In septic animals, p27 immunoreactivity was increased significantly in the nuclei of oocytes and decreased in the cytoplasm of granulosa and theca cells in multilaminar primary follicles compared to the sham group. In ultrastructural evaluation, increased apoptosis was observed in theca interna and granulosa cells in both the early and late stages of follicles in the CLP group. In conclusion, experimentally-induced sepsis leads to apoptosis in ovarian follicles at advanced stages of development. Our data suggest that although sepsis may not cause a potential threat to developing follicles at least in the short term, more severe damage may occur during advanced stages of follicle development.
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http://dx.doi.org/10.1016/j.repbio.2020.04.003DOI Listing
September 2020

Preliminary Comparison of Zero-Gravity Chair With Tilt Table in Relation to Heart Rate Variability Measurements.

IEEE J Transl Eng Health Med 2020 2;8:1900308. Epub 2020 Apr 2.

Department of Biomedical and Chemical Engineering and SciencesFlorida Institute of TechnologyMelbourneFL32901USA.

Heart rate variability (HRV) measurements are performed using a tilt-table (TT) to diagnose dysfunctionality in the autonomic nervous system (ANS) and the cardiovascular system. To maintain homeostasis, the ANS adapts to body position changes through alterations in sympathetic and parasympathetic responses that can be quantified by extracting time-domain and frequency-domain parameters from the heart rate signal. When body position is changed from supine to erect, a healthy subject's response also shows changes in ANS activity. However, TT can be unsafe or uncomfortable for elderly or overweight subjects. Furthermore, it may induce anxiety which alters the HRV measurements. This study proposes an alternative strategy to replace the TT with a zero-gravity chair (ZGC). The statistical analysis between HRV parameters from the TT and the ZGC shows that ZGC can be a feasible alternative to TT. Therefore, ZGC can be used as a more convenient, secure, stable and safer option to the traditional HRV analysis with TT.
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http://dx.doi.org/10.1109/JTEHM.2020.2983147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7166134PMC
April 2020

Isolated aneurysm of right sinus of Valsalva: A rare case report.

Turk Gogus Kalp Damar Cerrahisi Derg 2019 Apr 24;27(2):227-229. Epub 2019 Apr 24.

Department of Cardiovascular Surgery, University of Health Sciences, İstanbul Mehmet Akif Ersoy Thoracic and Cardiovascular Surgery Training and Research Hospital, Istanbul, Turkey.

Isolated sinus of Valsalva aneurysm is a rare congenital pathology associated with a life-threatening course when untreated. In this article, we report a 60-year-old male patient with an isolated aneurysm of right sinus of Valsalva treated by modified Bentall procedure. The patient with cardiac symptoms applied to our clinic for further evaluation. Enhanced computed tomographic angiography and transthoracic echocardiography revealed an isolated aneurysm of right sinus of Valsalva. An optimal management for the condition is not clear, while early surgical intervention is recommended due to potentially life-threatening complications.
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http://dx.doi.org/10.5606/tgkdc.dergisi.2019.17302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7021403PMC
April 2019

Tight junctions in the blood-brain barrier promote edema formation and infarct size in stroke - Ambivalent effects of sealing proteins.

J Cereb Blood Flow Metab 2021 01 13;41(1):132-145. Epub 2020 Feb 13.

Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin-Buch, Germany.

The outcome of stroke is greatly influenced by the state of the blood-brain barrier (BBB). The BBB endothelium is sealed paracellularly by tight junction (TJ) proteins, i.e., claudins (Cldns) and the redox regulator occludin. Functions of Cldn3 and occludin at the BBB are largely unknown, particularly after stroke. We address the effects of Cldn3 deficiency and stress factors on the BBB and its TJs. Cldn3 tightened the BBB for small molecules and ions, limited endothelial endocytosis, strengthened the TJ structure and controlled Cldn1 expression. After middle cerebral artery occlusion (MCAO) and 3-h reperfusion or hypoxia of isolated brain capillaries, Cldn1, Cldn3 and occludin were downregulated. In Cldn3 knockout mice (C3KO), the reduction in Cldn1 was even greater and TJ ultrastructure was impaired; 48 h after MCAO of wt mice, infarct volumes were enlarged and edema developed, but endothelial TJs were preserved. In contrast, junctional localization of Cldn5 and occludin, TJ density, swelling and infarction size were reduced in affected brain areas of C3KO. Taken together, Cldn3 and occludin protect TJs in stroke, and this keeps the BBB intact. However, functional Cldn3, Cldn3-regulated TJ proteins and occludin promote edema and infarction, which suggests that TJ modulation could improve the outcome of stroke.
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http://dx.doi.org/10.1177/0271678X20904687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747158PMC
January 2021

Design of a new dual mesh with an absorbable nanofiber layer as a potential implant for abdominal hernia treatment.

J Tissue Eng Regen Med 2020 02 30;14(2):347-354. Epub 2019 Dec 30.

Department of Polymer Engineering, Yalova University Faculty of Engineering, Yalova, Turkey.

Dual meshes are often preferred in the treatment of umbilical and incisional hernias where the abdominal wall defect is large. These meshes are generally composed of either two nonabsorbable layers or a nonabsorbable layer combined with an absorbable one that degrades within the body upon healing of the defect. The most crucial point in the design of a dual mesh is to produce the respective layers based on the structure and requirements of the recipient site. We herein developed a dual mesh that consists of two layers: a nanofibrous layer made of poly (glycerol sebacate)/poly (caprolactone) (PGS/PCL) to support the healing of the abdominal wall defect and a nondegradable, nonadhesive smooth layer made of polycarbonateurethane (PU) with suitable properties to avoid the adhesion of the viscera to the mesh. To prepare the double-sided structure, PGS/PCL was directly electrospun onto the PU film. This processing approach provided a final product with well-integrated layers as observed by a scanning electron microscope. Tensile test performed at the dry state of the samples showed that the dual mesh has the ability to elongate seven times more as compared with the commercially available counterparts, mimicking the native tissue properties. The degradation test carried out at physiological conditions revealed that PGS started to degrade within the first 15 days. in vitro studies with human umbilical vein endothelial cells demonstrated the double function of the meshes, in which PU layer did not allow cell adhesion, whereas PGS/PCL layer has the ability to support cell adhesion and proliferation. Therefore, the material developed in this study has the potential to be an alternative to the existing hernia mesh products.
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http://dx.doi.org/10.1002/term.3000DOI Listing
February 2020

The effects of magnesium sulfate on cyclophosphamide-induced ovarian damage: Folliculogenesis.

Acta Histochem 2020 Feb 4;122(2):151470. Epub 2019 Dec 4.

Istanbul University, Istanbul Faculty of Medicine, Department of Histology and Embryology, Istanbul 34093, Turkey. Electronic address:

Cyclophosphamide (CYP) is one of the alkylating chemotherapeutic agents and its adverse effects on folliculogenesis in the ovary are well-known due to the previous scientific research on this topic. Magnesium has various effects in organisms, including catalytic functions on the activation and inhibition of many enzymes, and regulatory functions on cell proliferation, cell cycle, and differentiation. In this study, the effects of magnesium sulfate (MgSO) on CYP induced ovarian damage were investigated. Immature Wistar-Albino female rats of 28-days were treated with pregnant mare serum gonadotrophin (PMSG) to develop the first generation of preovulatory follicles. Rats of the experimental groups were then treated with either CYP (100 mg/kg, i.p) and MgSO (270 mg/kg loading dose; 27 mg/kg maintenance doseX12, i.p) solely or in combination. Following in-vivo 5-bromo-2-deoxyuridine (BrdU) labeling, animals were sacrificed and ovaries were embedded in paraffin and Epon. In the ovaries, added to the evaluation of general morphology and follicle count; BrdU and TUNEL-labeling, cleaved caspase-3 and p27 (cyclin-dependent kinase inhibitor) staining was also performed immunohistochemically and an ultrastructural evaluation was performed by transmission electron microscopy (TEM). The number of primordial follicles were decreased and multilaminar primary and atretic follicles were increased in CYP group. After MgSO treatment, while primordial follicle pool were elevated, the number of atretic follicles were decreased. Additionally, decreased BrdU-labeling, increased cleaved caspase 3 immunoreactivity and increased TUNEL labeling were observed in CYP group. In CYP treated animals, observations showed that while MgSO administration caused no alterations in BrdU proliferation index and caspase-3 immunoreactivity, it significantly reduced the TUNEL labeling. It was also observed that, while p27 immunoreactivity significantly increased in the nuclei of granulosa and theca cells in the CYP group; MgSO treatment significantly reduced these immunoreactivities. The ultrastructural observations showed frequent apoptotic profiles in granulosa and theca cells in both early and advanced stages of follicles in the CYP group and the MgSO treatment before the CYP application led to ultrastructural alleviation of the apoptotic process. In conclusion, our data suggest that MgSO may provide an option of pharmacologic treatment for fertility preservation owing to the beneficial effects of on chemotherapy-induced accelerated follicular apoptotic process, and the protection of the primordial follicle pool.
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http://dx.doi.org/10.1016/j.acthis.2019.151470DOI Listing
February 2020

Efficacy and Safety of Paliperidone Palmitate Treatment in Patients With Schizophrenia: A Real-World Multicenter, Retrospective, Mirror-Image Study.

J Clin Psychopharmacol 2019 Nov/Dec;39(6):604-610

Department of Psychiatry, Marmara University School of Medicine, Istanbul, Turkey.

Purpose: The aim of the study was to assess efficacy and safety of paliperidone palmitate (PP) in schizophrenic patients using real-life data.

Methods: This national, multicenter, retrospective, and mirror-image study was performed reviewing the medical records of patients in 18 centers. Adult schizophrenic patients receiving PP treatment (n = 205) were enrolled. Patients' data covering the last 12 months before the initial PP injection and the period until the end of study with at least 12 months after the initial PP injection were evaluated. Patients' characteristics, scale scores, and adverse events were recorded.

Results: Nonadherence to prior medication was the most frequent reason for switching to PP treatment. Comparing with the period before PP treatment, the rate of patients visiting the hospital for relapse (79.5% vs 28.9%, P < 0.001) and the median number of hospitalizations (2 vs 0, P < 0.001) were lower during PP treatment. During PP treatment, the Positive and Negative Syndrome Scale score decreased by 20% or more (response to treatment) in 75.7% of the patients. The frequency of adverse events did not differ between the period before and during PP treatment. Improvement in functionality was higher in those with disease duration of 5 years or less.

Conclusions: Paliperidone palmitate is effective and safe in treatment of schizophrenic patients and in switching to PP treatment in patients with schizophrenia, which reduced the percentage of patients admitted to the hospital for relapse and the median number hospitalization, and has positive effects on functionality.
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http://dx.doi.org/10.1097/JCP.0000000000001133DOI Listing
April 2020
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