Publications by authors named "Mehmet Gamsizkan"

40 Publications

KRAS, BRAF, PIK3CA mutation frequency of radical prostatectomy samples and review of the literature.

Aging Male 2020 Dec;23(5):1627-1641

Department of Pathology, Duzce University, Duzce, Turkey.

Objective: The molecular basis of prostate cancer is highly heterogeneous. Our study aimed to perform the mutation analysis of , and immunohistochemical (IHC) evaluation of EGFR, HER2, p16, and PTEN to demonstrate new areas for targeted therapies.

Methods: A total of 24 prostatectomy samples diagnosed with adenocarcinoma were analyzed by microarray hybridization. Also, these samples were IHC stained for EGFR, HER2, P16, and PTEN. The cases were divided into two groups based on low and high Gleason scores. All findings were compared with the clinicopathological parameters of the patients.

Results: While mutation was in 3/24 (12.5%) of our cases, and mutations were not detected. There was no significant difference between the groups in terms of mutation frequency. HER2 was immunohistochemically negative in all samples. There was no correlation between EGFR, P16 immunopositivity, and clinicopathological features.

Conclusion: mutation frequency is similar to those in Asian populations. and mutation frequencies have been reported in the literature in the range of 0-15% and 0-10.4%, respectively, consistent with our study findings. HER2 immunoexpression is a controversial issue in the literature. EGFR and p16 expressions may not correlate with the stage.
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http://dx.doi.org/10.1080/13685538.2021.1901274DOI Listing
December 2020

Intraoperative Dermatoscopic Features of Subungual Exostosis.

Arch Bone Jt Surg 2020 Nov;8(6):757-759

Department of Pathology, Faculty of edicine, Düzce University, Düzce, Turkey.

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http://dx.doi.org/10.22038/abjs.2020.45293.2239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718573PMC
November 2020

Systemic tranexamic acid promotes bone healing in a rat model of femur fracture.

Jt Dis Relat Surg 2020 ;31(3):432-439

Düzce Üniversitesi Tıp Fakültesi Ortopedi ve Travmatoloji Anabilim Dalı, 81110 Düzce, Türkiye.

Objectives: This study aims to investigate the effect of tranexamic acid (TXA) on the union of closed femoral fractures using radiological and histological methods in an experimental rat model.

Materials And Methods: This experimental study was conducted between June 2017 and February 2018. Closed femoral fractures were created in 36 male Wistar albino rats (age: three months [range, 2.5-3.5 months], weighing 200 grams [range, 180-220 grams]). Half of the animals randomly divided into two groups were administered intravenous single dose of TXA (30 mg/kg), whereas the animals in the control group did not receive any medication. The animals in the two groups were randomly divided into three groups with six animals each and cervical dislocation was performed at days 15, 30, and 45, and radiological and histopathological healing scores were compared.

Results: When the mean radiological scores of the TXA and control groups were compared, a statistically significant difference was found in favor of the TXA group at day 15 (p=0.019), but no significant difference was found in the mean scores on days 30 and 45 (p=0.138 and p=0.269, respectively). Histopathological examination also showed a statistically significant difference between the 15-day mean score values in favor of the TXA group ( p = 0. 017 ).

Conclusion: The use of systemic TXA accelerates early bone formation and fracture healing.
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http://dx.doi.org/10.5606/ehc.2020.75430DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607943PMC
February 2021

Short- and long-term effects of rapamycin on ischemic damage and apoptotic changes in torsion of rat testes.

Naunyn Schmiedebergs Arch Pharmacol 2021 01 19;394(1):85-94. Epub 2020 Aug 19.

Department of Medical Genetics, Duzce University Medical Faculty, Duzce, Turkey.

Rapamycin has antioxidant defense mechanisms and immune suppressive effects. To detect the short- and long-term effects of rapamycin on ischemic damage and apoptotic changes in torsion of rat testes, mature male albino Wistar rats (n = 48) were included in the study as control, sham, early torsion-detorsion (T/D), early rapamycin treatment, early rapamycin control, late T/D, late rapamycin treatment, and late rapamycin control. The right testis was rotated 720° in a clockwise direction during 4 h in operation groups. Rapamycin was administered orally three times: 30 min before detorsion and 24 and 48 h after detorsion. The animals were killed on the third day in early groups and on the tenth day in late groups after detorsion. Statistically significant differences among all groups were detected for SOD and TBARS, mean seminiferous tubule diameter (MSTD) and Cosentino's histologic score (CHS), caspase 3, bax, average number of apoptotic cells per tubule (ANPCT), and percentage of apoptotic tubule (PAT) values. ANPCT values ​​were 10% lower in the rapamycin treatment groups compared with the untreated T/D groups, and the PAT values ​​were also approximately 1.3 times lower. Although short-term usage of rapamycin may reduce to the tubular injury caused by I/R conversely to apoptosis in the testicular tissue after testicular torsion, rapamycin may have the potential to increase the long-term apoptosis with/without testicular torsion and a subsequent regression in fertility.
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http://dx.doi.org/10.1007/s00210-020-01965-4DOI Listing
January 2021

BRAF mutation, TERT promoter mutation, and HER2 amplification in sporadic or neurofibromatosis-related neurofibromas and malignant peripheral nerve sheath tumors: do these molecules have a signature in malignant transformation?

APMIS 2020 Sep 29;128(9):515-522. Epub 2020 Jul 29.

Department of Pathology and Cytology, School of Medicine, Duzce University, Duzce, Turkey.

Peripheral nerve sheath tumors may occur sporadically or related to neurofibromatosis (NF). Unless the mechanisms of tumorigenesis in NF related malignant peripheral nerve sheath tumors (MPNST) are better understood, it remained unclear in sporadic cases. We aimed to investigate the genetic route for malignancy in both individuals with NF-1 and sporadic ones to open a way for targeted therapies in the future. We investigated the role of HER2 with Dual ISH DNA Probe Cocktail test, BRAF mutation (exon 15) and TERT promoter mutation frequency with Sanger sequencing method in respectively 25 sporadic neurofibromas, 25 NF-1 related neurofibromas and 25 MPNST cases from two institutes. Categorical data were analyzed and summarized as frequency and percentage. Statistical analysis was done with SPSS v.22 statistical package, and the statistical significance level was considered as 0.05. We identified TERT promoter mutation only in one sporadic MPNST (4%) and no BRAF mutation in any case. HER2 amplification is found in 10/25 (40%) MPNST cases. No mutations or gene amplification detected in neurofibromas (p < 0.001). MPNSTs are sarcomas with poor prognosis and limited treatment options. TERT promoter mutations and HER2 amplification may play a putative role in therapeutic purposes.
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http://dx.doi.org/10.1111/apm.13063DOI Listing
September 2020

Novel mutation identified in the DDB2 gene in patients with xeroderma pigmentosum group-E.

Int J Dermatol 2020 Aug 12;59(8):989-996. Epub 2020 Jun 12.

Duzce University Medical School, Duzce, Turkey.

Background: Xeroderma pigmentosum (XP) is a rare photosensitive syndrome, which is divided into eight complementation groups (XP-A to XP-G and XPV) and characterized by skin cancers diagnosed at early age. A family of seven members (age range between 5 and 47 years) with carriers of the novel nonsense mutation that causes XP-E type were included in the current study.

Methods: DNA was isolated from peripheral blood samples of the proband, and cancer predisposition genes were sequenced with next-generation sequencing. The demographic features and the laboratory, clinical, and histopathological findings of patients were evaluated.

Results: In the proband, squamous cell carcinoma was first diagnosed in the right-eye cornea at the age of 13 years and then in the left-eye cornea at the age of 15 years. Later, the patient was diagnosed with basosquamous cell carcinoma on the dorsum of the nose at the age of 18 years. After genetic analysis, a novel nonsense c.1063C>T(p.Arg355Ter) pathogenic variation that causes XP-E type was detected as homozygous in the DDB2 gene of the proband and her siblings, 11 and 5 years of age, and as heterozygous in her parents and a 22-year-old brother.

Conclusion: Because of the occurrence of early termination codon, truncated nonfunctional proteins or proteins with deleterious loss or gain-of-function activities are synthesized in nonsense mutation. Thus, to avoid the development of pathological lesions, it is important that such patients with nonsense mutation stay away from agents that might cause DNA damage and develop an appropriate lifestyle according to this condition.
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http://dx.doi.org/10.1111/ijd.14957DOI Listing
August 2020

A rare localized giant cell tumor of the tendon sheath originating from the ligamentum mucosum: A case report.

Jt Dis Relat Surg 2020 ;31(1):149-53

Department of Orthopedics and Traumatology, Düzce University Faculty of Medicine, Düzce, Turkey.

Giant cell tumor of the tendon sheath (GCTTS) occurs most often in the hand and rarely in the feet, and as an extremely uncommon presentation in the knee joint. Case reports involving GCTTS in the knee joint generally describe it originating from the nearby anterior cruciate ligament, posterior cruciate ligament, patellar tendon, and medial plica. To the best of our knowledge, there are no previously reported case reports involving GCTTS originating in the ligamentum mucosum. In this article, we describe a 27-year-old male patient who was admitted to the orthopedic emergency room with a painful locked knee. He had severe pain that was worse with activity and a decreased range of motion. Magnetic resonance imaging (MRI) indicated massive swelling and a well-circumscribed lobulated intraarticular mass at the distal one third of the ligamentum mucosum. The mass was removed successfully with arthroscopic-assisted mini-open excision, and histological analysis subsequently diagnosed it as a localized type of GCSTT. The patient remained asymptomatic and a follow-up MRI two years after surgery did not show any recurrence of the lesion.
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http://dx.doi.org/10.5606/ehc.2020.72323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489121PMC
August 2020

Anti-TNF agent etanercept augments UV-induced skin cancer development in SKH-1 mice.

J Dermatolog Treat 2020 Jan 7:1-7. Epub 2020 Jan 7.

Department of Dermatology, Gulhane School of Medicine, Health Sciences University, Ankara, Turkey.

Despite being employed in the treatment of inflammatory disorders for more than 20 years all over the world, data regarding photocarcinogenic risks of anti-TNF agents is scarce. To assess photocarcinogenic potential of anti-TNF agents. This was a placebo controlled, split-body (UVB-treated versus -untreated) study on mice. Treatment groups were infliximab ( = 11), etanercept ( = 11), cyclosporine ( = 11) and vehicle control ( = 11). Agents were introduced on the 10th week of phototherapy and continued through 24th week. The macroscopic, histological and immunohistochemical analysis of test sites were carried out. Overall 132 tumors were detected on test sites. All of these tumors developed on UV-exposed sides. Histologic examination of these tumors was compatible with keratinocytic neoplasia in 128, mastocytosis in 3, epidermal cyst in 1. Median tumor burden in the UVB exposed areas for ETN, IFX, CYC, and control groups were 14.91, 10.20, 6.28, and 3.14 cm, respectively. ETN group demonstrated both higher tumor burden and keratinocytic neoplasia numbers than controls ( = .03,  = .025). Although there were 1.8 and 1.7 times more keratinocytic neoplasms in IFX and CYC groups compared to controls, these differences didn't reach statistically significant levels ( = .14;  = .19). This study points out to a significant photocarcinogenic potential of anti-TNF agent etanercept.
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http://dx.doi.org/10.1080/09546634.2019.1708851DOI Listing
January 2020

Chondroprotective effect of oil in the early stages of osteoarthritis: an experimental study in rabbits.

J Musculoskelet Neuronal Interact 2019 09;19(3):362-369

Orthopedics and Traumatology Department, Medeniyet University Medical Faculty, Istanbul, Turkey.

Purpose: oil possesses a well-known ability to protect certain organs from oxidative, neoplastic, and inflammatory damage. This study investigated the potential chondroprotective effects of intraarticular injections of oil in a rabbit osteoarthritis model.

Methods: Osteoarthritis models were created by performing anterior cruciate ligament transections in 20 New Zealand rabbits. Rabbits were randomly divided into two groups of 10 and given intraarticular injections in their right knees weekly for 5 weeks, beginning in the third week post-operation. Injections given to the first group contained whole oil, whereas the second group was injected with a saline solution. Knee joints were harvested 8 weeks after surgery. Knee joint surfaces were examined macroscopically, and medial femoral condyle sections were examined microscopically.

Results: There was a statistically significant difference in the macroscopic grading results of the groups, with the group having better results (p=0.001). The a group also received significantly better total Osteoarthritis Research Society International (OARSI) scores (p=0.035).

Conclusions: Intraarticular administration of oil has the potential to protect cartilage from degeneration in the early stages of osteoarthritis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737550PMC
September 2019

Cryosurgery to remove perichondrium for the rabbit ear hypertrophic scar model: a simplified method.

Acta Dermatovenerol Alp Pannonica Adriat 2019 06;28(2):57-59

Department of Dermatology, Gülhane Medical Faculty, University of Health Sciences, Ankara, Turkey.

Introduction: The rabbit ear hypertrophic scar model is a preferred animal model of excessive scarring for investigating the scarring process and novel treatment modalities. In this model, surgical removal of perichondrium can be challenging, and it is often insufficient or damages the underlying cartilage. It is hypothesized that cryosurgery would offer a more efficient alternative to conventional surgery. The objective of this study was to compare structural changes in scar tissues in two groups of the hypertrophic scar model: cryosurgery compared to standard conventional surgery.

Methods: We introduced a novel technique to remove perichondrium using cryosurgery. Hypertrophic scars obtained with conventional surgery and cryosurgery were studied in a left-right comparison method. Comparative parameters included the histological structure of the scars and structural changes in the cartilage just beneath the scarring.

Results: Cryosurgery produced similar scars in comparison to conventional surgery. Although statistically not significant (p = 0.16), the histological findings of cartilage damage were lower in the cryosurgery group (six out of 21) compared to the established model (10 out of 20).

Conclusion: This study suggests that cryotherapy can be used for removal of perichondrium.
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June 2019

Effects of acidic and nonacidic reflux on the eustachian tube: An animal experiment.

Ear Nose Throat J 2018 Dec;97(12):E21-E27

MediENT Ear Nose and Throat Hospital, Halk Sokak no:50/1, Istanbul, Turkey.

We investigated the effects of pepsin/hydrochloric acid and bile acids on eustachian tube function and eustachian tube mucosa in 40 Sprague-Dawley rats. The animals were randomly assigned to groups of 10: one group received pepsin/hydrochloric acid (pepsin/HCl group), another received human bile (human bile group), a third received a mixture of pepsin/HCl and human bile (combination group), and the fourth received isotonic saline solution (control group). Test solutions were applied transnasally three times a day for 10 days. Passive opening pressures and passive closing pressures were measured digitally at baseline and then on days 3, 5, and 10. After 10 days, the rats were sacrificed and histologic changes in the eustachian tube mucosa were analyzed. At study's end, we observed that the increases in passive opening pressures and passive closing pressures in all three experimental groups were significantly greater than those of the control group. Moreover, the increases in passive opening and closing pressures were significantly greater in the combination group than in both the pepsin/HCl and the human bile groups. In the tympanic orifice, the degree of lymphocyte and polymorphonuclear leukocyte infiltration was significantly higher in all three experimental groups than in the control group. In the nasopharyngeal orifice, lymphoid follicle formation was significantly more common in the human bile group than in the control group; also, the presence of subepithelial vasodilation and subepithelial edema was significantly more common in the pepsin/HCl and combination groups than in the controls. Of the three experimental solutions tested, the combination of pepsin/HCl and human bile was the most injurious to eustachian tube function. Reflux of bile acids causes eustachian tube dysfunction, and this damage worsens with the introduction of an acidic compound.
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December 2018

Investigation of the preventive effect of proanthocyanidin in ischemia-reperfusion injury in lung transplantation: An experimental study.

Turk Gogus Kalp Damar Cerrahisi Derg 2018 Oct 16;26(4):606-613. Epub 2018 Sep 16.

Department of Thoracic Surgery, University of Health Sciences, Gülhane Training and Research Hospital, Ankara, Turkey.

Background: This study aims to investigate the preventive effect of proanthocyanidin against ischemia-reperfusion injury after lung transplantation.

Methods: The study included 12 swines (weighing 35±5 kg) and separated into four groups. Groups 1 and 3 were identified as control groups and left upper lobectomy was performed. Groups 2 and 4 were identified as transplantation groups and left lower lobectomy and heterotransplantation were performed. Proanthocyanidin was only given to groups 3 and 4. Tissue samples were analyzed under light microscope and histopathological findings were recorded.

Results: There was no statistically significant difference between control groups in terms of the numerical values of histopathological findings that include congestion (p=0.565), alveolar edema (p=0.197) and peribronchial inflammation (p=0.444). However, numerical values of acute cellular rejection were statistically significantly different between transplantation groups (p=0.048). Mean oxidative stress enzyme levels were higher in group 2 compared to group 4; however, the difference was not statistically significant (p>0.05).

Conclusion: According to the findings of our experimental study, proanthocyanidin can be safely used in lung transplantation based on its preventive effect in ischemia-reperfusion injury that may lead to morbidity and mortality.
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http://dx.doi.org/10.5606/tgkdc.dergisi.2018.16078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7018171PMC
October 2018

Talar Osteochondroma Fracture Presenting as Posterior Ankle Impingement.

J Am Podiatr Med Assoc 2016 May;106(3):225-8

Osteochondromas are the most common benign bone tumors. They are usually asymptomatic and found incidentally. When symptomatic, the symptoms are usually due to its location and size. Fracture of an osteochondroma presenting as posterior ankle impingement is a rare condition. We describe a 22-year-old man with solitary exostosis who presented with a posterior ankle mass and posterior ankle impingement with 2 years of follow-up. Surgical intervention was the treatment of choice in this patient, and histologic examination revealed a benign osteochondroma. Osteochondromas found in the posterior aspect of the talus can be complicated by fracture due to persistent motion of the ankle. Talar osteochondroma should be included in the differential diagnosis of posterior ankle impingement causes. Posterior talar osteochondromas, especially when a stalk is present, should be treated surgically before it is more complicated by a fracture and posterior ankle impingement.
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http://dx.doi.org/10.7547/15-001DOI Listing
May 2016

Mutation analysis of metastatic melanomas in the central nervous system: results of a panel of 5 genes in 48 cases.

Clin Neuropathol 2016 Jul-Aug;35(4):178-85

Melanocytic lesions in the central nervous system (CNS) may be primary to the site but are more commonly metastases from cutaneous primaries. In fact, melanomas are one of the most common malignancies that can metastasize to the brain, and some patients may not have a diagnosis of melanoma prior to the discovery of the CNS lesion. In such cases, identifying the primary site may be challenging. We reviewed the archives of a large referral center for melanocytic tumors involving the CNS and selected 48 patients for this study based on our inclusion criteria. We used sequencing to identify mutation status of these tumors and compared these with clinicopathological features. Mutations in exon 9, 11, 13, 17, and 18 of KIT gene, exon 15 of BRAF gene, exon 2 and 3 of NRAS gene, exon 4 and 5 of GNAQ and GNA11 genes were analyzed. Mutations in BRAF-exon 15 were the most common among tumors (58.3%). NRAS-exon 2 and NRAS-exon 3 mutations were detected in 3 and 7 cases, respectively. GNAQ-exon 4, GNAQ-exon 5 and GNA11-exon 5 mutation were present in 1 tumor each. Eight tumors were wild type for all 5 genes, and 6 of these were not known primary despite a work-up and clinical follow-up. Only 1 of these tumors showed a mutation in exon 11 of KIT gene. When compared to primary melanocytic lesions of the CNS, metastatic melanomas were characterized by BRAF gene mutations and wild-type GNAQ and GNA11 genes.
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http://dx.doi.org/10.5414/NP300941DOI Listing
January 2017

Nevus sebaceus with basal cell carcinoma, poroma, and verruca vulgaris.

Indian J Pathol Microbiol 2015 Oct-Dec;58(4):534-6

Department of Pathology, Gülhane Military Medical Academy, Ankara, Turkey.

Nevus sebaceus (NS) is a congenital, benign, hamartomatous lesion and it is possible to see several benign or malignant tumors accompanying it. One of these is the poroma, which is very rare, and has only been reported twice before, in the English literature. In this paper, we presented two new cases of NS. One of them was a 40-year-old male who presented with a congenital skin lesion on his temporoparietal region. This lesion was composed of four different lesions, including NS, poroma, basal cell carcinoma (BCC), and verruca vulgaris. The second patient was a 41-year-old male presenting with a yellow-brown patch on the scalp. This lesion was comprised of NS and BCC. In addition to these presentations, we discussed the differential diagnosis between BCC and trichoblastoma, both of which are likely to be seen with NS. For this purpose, we recommended an immunohistological panel, which may be useful for differentiating these two morphologically similar lesions.
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http://dx.doi.org/10.4103/0377-4929.168885DOI Listing
April 2017

Molecular alterations in malignant blue nevi and related blue lesions.

Virchows Arch 2015 Dec 24;467(6):723-732. Epub 2015 Sep 24.

Istanbul Faculty of Medicine, Department of Pathology, Istanbul University, Istanbul, Turkey.

Malignant blue nevi (MBN) are extremely rare dermal melanocytic tumors that arise in association with atypical cellular blue nevi (ACBN), cellular blue nevi (CBN), common blue nevi (BN), or de novo. The frequency of BRAF, NRAS, and KIT mutations in malignant melanoma varies according to histological subtype and localization. These mutations are rarely observed in blue nevi, which have recently been shown to carry activating mutations in GNAQ/GNA11 genes. Only few small molecular studies of MBN have been published. The aim of the present study was to analyze in MBN and related blue lesions such as ACBN, CBN, and BN the prevalence of BRAF, NRAS, KIT, GNAQ, and GNA11 gene mutations and their association with clinicopathological features. We included in our study 12 MBN, 6 ACBN, 29 CBN, and 35 common BN diagnosed between 1996 and 2014. Sanger sequencing method was used for mutation analysis. Overall, GNAQ exon 5 mutation was the most frequent alteration (46 %), in 2 of 12 (17 %) MBN, 1 of 6 (17 %) ACBN, 22 of 29 (76 %) CBN, and 13 of 35 (37 %) common BN. BRAF V600E and GNA11 exon 5 mutations were respectively detected in 3 of 12 (25 %) and in 2 of 12 (17 %) MBN while none in ACBN, CBN, and common BN. None of the cases harbored NRAS exon 2/3, KIT exon 9/11/13/17/18, or GNAQ/GNA11 exon 4 mutations. GNAQ gene exon 5 mutations are rare in MBN and ACBN but frequent in CBN and common BN. Remarkably, BRAF V600E and GNA11 exon 5 mutations were only detected in MBN, whereas none were found in ACBN, CBN, or common BN. Our data contribute new elements to the limited data on molecular alterations in MBN.
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http://dx.doi.org/10.1007/s00428-015-1851-3DOI Listing
December 2015

BRAF, KIT, NRAS, GNAQ and GNA11 mutation analysis in cutaneous melanomas in Turkish population.

Indian J Pathol Microbiol 2015 Jul-Sep;58(3):279-84

Department of Pathology, Gulhane Military Medical Academy, Haydarpasa Training Hospital, Istanbul, Turkey.

Background: KIT and mitogen-activated protein kinase cascade are important for melanomagenesis. In the present study, we analyzed the frequency of BRAF, NRAS, KIT, GNAQ and GNA11 gene mutations and investigated their association with clinicopathological features of melanomas in Turkish population.

Materials And Methods: Forty-seven primary cutaneous melanomas were included in our study. Sanger sequencing method was used for mutation analysis in all cases.

Results: Mean age was 62.1 (29-101) years. Female:male ratio was 17:30. Among 47 melanomas, 14 (29.8%) BRAF, 10 (21.3%) NRAS, 4 (8.5%) KIT and 1(2.1%) GNAQ gene mutations were detected. Two of the KIT mutations were found in acral lentiginous melanoma (ALM). In the head and neck region, mutation frequency was significantly lower than in other locations (P = 0.035). The only GNAQ gene mutation (p.Q209L) was detected in a melanoma arising from blue nevus located on the scalp. None of the melanomas harbored NRAS exon 2, KIT exon 13/17/18, GNAQ exon 4 and GNA11 exon 4/5 mutations. Overall mutation frequency did not show significant difference between metastatic (8/14, 57.1%) and nonmetastatic (18/33, 54.5%) patients. We did not observe any significant association between mutation status and gender or age of various patients.

Conclusions: Our results support that BRAF and NRAS gene mutations are common in cutaneous melanomas. The activating mutations of KIT gene are rare and especially seen in ALM. GNAQ and GNA11 mutations are infrequent in cutaneous melanomas and may be associated only with melanomas arising from blue nevus.
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http://dx.doi.org/10.4103/0377-4929.162831DOI Listing
April 2016

A retrospective multicenter evaluation of cutaneous melanomas in Turkey.

Asian Pac J Cancer Prev 2014 ;15(23):10451-6

Department of Pathology, Maresal Cakmak Military Hospital, Erzurum, Turkey E-mail :

Background: We defined melanoma distribution in a large series of Turkish patients and evaluated the prognostic parameters of melanomas.

Materials And Methods: A total of 1574 patients' data was retrospectively collected at 18 centers in Turkey. Demographic characteristics were questioned and noted. Prognostic parametres were evaluated based on sentinel lymph node involvement.

Results: Mean age was 56.7 (4-99) years. While 844 (53.6%) cases were male, 730 (46.4%) cases were female. One thousand four hundred forty-seven (92%) cases were invasive melanoma and 127 (8%) cases were in-situ melanoma. The most common histopathological form was the superficial spreading melanoma (SSM) which was found in 549 patients (37.9%). It was followed by nodular melanoma in 379 (26.2%), acral lentiginous melanoma (ALM) in 191 (13.2%) and lentigo maligna melanoma in 132 (9.1%), respectively. On univariate analysis, lymphovascular invasion (p<0.001), tumor thickness (p<0.001), histopathological subtype (p<0.001), Clark level (p=0.001), ulceration (p<0.001), ≥6/mm2 mitosis (p=0.005), satellite formation (p=0.001) and gender (p=0.03) were found to be associated with sentinel lymph node positivity. Regression was associated with sentinel lymph node negativity (p=0.017). According to multivariate analysis, lymphovascular invasion and tumor thickness were significant independent predictive factors of SLN positivity. Patient age, tumor localization, precursor lesions, lymphocytic infiltration and neurotropism were not related with sentinel lymph node involvement.

Conclusions: In this retrospective analysis, it was found that the prevalence of SSM is at a lower rate while the prevalence of ALM is at a higher rate when compared to western countries. According to Breslow index; most of the melanoma lesions' thickness were greater than 2 mm, corresponding Clark IV. Vascular invasion and tumor thickness are the most important factors for sentinel lymph node involvement.
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http://dx.doi.org/10.7314/apjcp.2014.15.23.10451DOI Listing
September 2015

A case of Marshall's syndrome and review of the literature.

Int J Dermatol 2015 Jun 10;54(6):e217-21. Epub 2014 Sep 10.

Department of Dermatology, Gülhane School of Medicine, Gülhane Military Medical Academy, Ankara, Turkey.

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http://dx.doi.org/10.1111/ijd.12471DOI Listing
June 2015

A case of atypical scleromyxedema without gammopathy treated with cyclosporine.

Indian J Dermatol Venereol Leprol 2014 May-Jun;80(3):278

Department of Dermatology, Gülhane School of Medicine, Ankara, Turkey.

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http://dx.doi.org/10.4103/0378-6323.132272DOI Listing
December 2014

Confluent and reticulated papillomatosis (Gougerot-Carteaud syndrome) in two brothers.

Acta Dermatovenerol Croat 2014 ;22(1):57-9

Gürol AÇIKGÖZ, MD Assistant Professor of Dermatology Department of Dermatology, Gülhane School of Medicine Etlik, Ankara 06018,Turkey;

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August 2015

An unusual infiltrative basal cell carcinoma with osteoclastic stromal changes mimicking carcinosarcoma: a case report.

Am J Dermatopathol 2015 Jan;37(1):26-30

*Department of Pathology, Erzurum Military Hospital, Erzurum, Turkey; †Departments of Pathology and Dermatology, Dermatopathology Service, University of California-San Francisco, San Francisco, CA; and ‡Department of Pathology, Eskisehir Military Hospital, Eskisehir, Turkey.

A 91-year-old man presented with an ulcerated nodule on his left lower eyelid. The tumor showed an epithelial component composed of basaloid and clear cells and a stroma that contained many osteoclastic giant cells. Strong, diffuse expression for cytokeratin 17 and p63 was noted in the epithelial component, whereas no staining was present in the sarcomatoid stroma, suggesting that the osteoclast-rich stromal component represented an unusual benign stromal reaction to the carcinoma rather than a manifestation of carcinosarcoma. Further supporting this interpretation was the absence of mitotic figures and low Ki-67 proliferation index (of approximately 1%) in the stromal cells. We herein reported a case of unusual infiltrative basal cell carcinoma, accompanied by a clear cell carcinomatous features and concurrent benign osteoclastic stromal changes.
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http://dx.doi.org/10.1097/DAD.0000000000000131DOI Listing
January 2015

Minor salivary gland neoplasms.

J Craniofac Surg 2014 May;25(3):e289-91

From the Departments of *Otolaryngology, Head and Neck Surgery, and †Pathology, Gulhane Military Medical School, Ankara, Turkey.

Objective: This study aimed to investigate the clinical presentation, histopathologic and epidemiological aspects, as well as the treatment modalities and outcomes of patients with minor salivary gland tumors (MSGTs).

Subjects And Methods: A series of 23 patients with MSGTs were reviewed retrospectively.

Results: This study included 11 (48%) benign and 12 (52%) malignant tumors of minor salivary glands. Minor salivary gland tumors were more common in men (70%) than in women (30%). The mean age was 31.3 years for benign tumors and 46.3 years for malignant tumors. Pleomorphic adenoma was the most common benign tumor, followed by myoepithelioma. Mucoepidermoid carcinoma and adenoid cystic carcinoma were the most common malignant tumors. The most common symptom was a painless mass of the palate. Surgical treatment was performed in all patients. Adjuvant radiotherapy was used in 3 malignant tumors. Twenty-three patients were followed-up for a median of 5 years. Two patients with malignant tumors underwent a second surgery for postoperative local recurrence. They were successfully treated with the second surgery.

Conclusions: Minor salivary gland tumors are relatively uncommon neoplasms of the head and neck region. There is limited literature on MSGTs. This study provides a versatile approach for MSGTs from demographic data and clinical presentations to treatment modalities and treatment outcomes.
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http://dx.doi.org/10.1097/SCS.0000000000000723DOI Listing
May 2014

Calcifying fibrous tumor: a case report.

Turk Patoloji Derg 2015 ;31(2):141-4

Department of Pathology, Gülhane Military Medical Academy, Faculty of Medicine, ANKARA, TURKEY.

Calcifying fibrous tumors are rarely seen and affect mostly children and young adults. A 21-year-old man presented with multiple palpable masses in the area from the right inguinal region to the anteromedial thigh. We performed a diagnostic excisional biopsy. Histopathologically, it was composed of fibroblasts, psammoma bodies, dystrophic calcifications and foci of mononuclear inflammatory cell infiltration in a collagenous dense stroma. We herein reported a case of calcifying fibrous tumor and discussed its clinical and morphological features with regard to the literature.
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http://dx.doi.org/10.5146/tjpath.2013.01216DOI Listing
January 2016

Taurine attenuates lung ischemia-reperfusion injury after lung transplantation in rats.

J Anesth 2014 Jun 6;28(3):347-53. Epub 2013 Nov 6.

Department of Anaesthesiology, Gazi University, Ankara, Turkey.

Purpose: Taurine, the major intracellular free amino acid found in high concentrations in mammalian cells, is known to be an endogenous antioxidant and a membrane-stabilizing agent. It was hypothesized that taurine may be effective in reducing ischemia-reperfusion injury after lung transplantation and an experimental study was conducted in a rat model.

Methods: The number of Sprague-Dawley rats used in the study was 35. Animals were randomized into five groups of 7 rats each, including control, donor I, donor II, ischemia-reperfusion injury, and treatment groups. All animals were exposed to the same experimental conditions in the preoperative period. Rats were fixed in a supine position after the induction. After the rats were shaved, a left pneumonectomy was performed following sternotomy in control, donor I, and donor II groups. The harvested grafts in donor I and donor II groups were transplanted to the rats of the ischemia-reperfusion group and treatment group, respectively. However, taurine was administered intraperitoneally for 3 days before the harvesting procedure in donor II. All harvested lungs were kept in a Euro-Collins solution at +4 °C for 24 h in a half-inflated manner. After harvesting and transplantation, lungs were sampled for histopathological and biochemical analysis.

Results: Malondialdehyde and superoxide dismutase, glutathione peroxidase, and catalase levels were lower in the treatment group than the other groups (p < 0.05). Histopathological findings were better in treatment group than the ischemia-reperfusion group (p < 0.05).

Conclusion: It was demonstrated that donor treatment with taurine resulted in preservation of transplanted lung tissue in respect to histopathological and biochemical findings.
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http://dx.doi.org/10.1007/s00540-013-1741-2DOI Listing
June 2014

Submandibular triangle masses.

J Craniofac Surg 2013 ;24(5):e529-31

From the * Departments of Otolaryngology, Head and Neck Surgery, and †Pathology, Gulhane Military Medical School, Ankara, Turkey.

Objective: The study aims to analyze the demographic data of a large case series operated on because of submandibular triangle mass for more than 10 years and presents a review of the literature.

Materials And Methods: The charts of patients who underwent surgical intervention for submandibular triangle mass between January 2000 and November 2010 were reviewed. The medical history, age, sex, duration of symptoms, clinical presentation, preoperative investigations, and histopathologic diagnosis were reviewed.

Results: The study included 66 subjects; 12 patients (18.2%) with submandibular sialolithiasis, 18 patients (27.2%) with sialadenitis, 10 patients with lymphadenitis (15.1%), and 26 patients (39.3%) with tumors. Of the tumors, 23% was malignant and 77% was benign. Benign tumors of submandibular gland comprised 22.7% and malign tumors of submandibular gland comprised 3% of all submandibular mass. The most common benign tumor was pleomorphic adenoma. The most frequent histopathologic diagnoses of submandibular masses originated from the submandibular gland, and these comprised 71.2% of all submandibular mass pathologies. The main symptom was a painless mass. Ultrasonography was the most common preoperative diagnostic procedure. Fine-needle aspiration biopsy was performed in 26 patients. A clear diagnosis could not be provided in 3 (12%) patients.

Conclusion: Infectious conditions and benign tumors are more frequent than malign tumors in the submandibular region. The histopathologic diagnoses mainly consisted of submandibular sialadenitis, sialolithiasis, pleomorphic adenoma, and lymphadenitis. Ultrasonography is the first option of radiologic evaluation. Fine-needle aspiration biopsy is a very useful and usually sufficient diagnostic procedure for histopathologic diagnosis. Excisional biopsy can be performed when the fine-needle aspiration biopsy failed.
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http://dx.doi.org/10.1097/SCS.0b013e3182a238f9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3781294PMC
September 2014

The effects of hyperbaric oxygen therapy on experimental colon anastomosis after preoperative chemoradiotherapy.

Int Surg 2013 Jan-Mar;98(1):33-42

1 Department of Surgery.

The aim of the present study was to investigate the effect of hyperbaric oxygen therapy (HBOT) on colon anastomosis after chemoradiotherapy (CRT). Sixty female Wistar-Albino rats were divided into 5 groups and underwent left colon resection and end-to-end anastomosis. CRT simulation was performed on 2 sham groups before the anastomosis, and 1 of these groups was administered additional postoperative HBOT. Two groups were administered CRT before the anastomosis, and 1 of them received additional postoperative HBOT. On postoperative day 5, all groups underwent relaparotomy; burst pressure was measured and samples were obtained for histopathologic and biochemical analysis. There was a significant weight loss in the CRT groups and postoperative HBOT had an improving effect. Significantly decreased burst pressure values increased up to the levels of the controls after HBOT. Hydroxyproline levels were elevated in all groups compared to the control group. Hydroxyproline levels decreased with HBOT after CRT. No significant difference was observed between the groups regarding fibrosis formation at the anastomosis site. However, regression was observed in fibrosis in the group receiving HBOT after CRT. Preoperative CRT affected anastomosis and wound healing unfavorably. These unfavorable effects were alleviated by postoperative HBOT. HBOT improved the mechanical and biochemical parameters of colon anastomosis in rats.
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http://dx.doi.org/10.9738/CC130.2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3723167PMC
August 2014

Intraosseous tophus deposits in the os trigonum.

Orthopedics 2012 Jan 16;35(1):e120-3. Epub 2012 Jan 16.

Department of Orthopaedics and Traumatology, Bakirkoy Dr Sadi Konuk Education and Research Hospital, Tevfik Saglam cad. No: 11, 34147,Zuhuratbaba, Istanbul, Turkey.

High levels of uric acid cause accumulation of monosodium urate crystals. This formation of masses is called tophus. Intraosseous tophus deposits are rare, even for patients with gout. We report an unusual case of intraosseous tophus deposits in the os trigonum. The patient presented with ankle pain with no previous history of gout. On examination, tenderness on the posterior aspect of his ankle and limitation of plantarflexion was noted. Laboratory values were normal, except for an elevated serum uric acid value. Radiographs of the right ankle showed the presence of a large os trigonum with osteosclerotic changes, whereas magnetic resonance imaging showed intraosseous tophus deposits in the os trigonum. Conservative therapy failed, and the patient was admitted for an endoscopic resection of the os trigonum.Intraosseous chalky crystals were detected during endoscopic resection of the os trigonum. The histological diagnosis was tophaceous gout. The underlying pathological mechanism of intraosseous tophi is uncertain. Penetration of urate crystals from the joint due to hyperuricemia may be the mechanism of deposition in this patient.When a patient with hyperuricemia presents with posterior ankle impingement symptoms, intraosseous tophus deposits should be included in the differential diagnosis. Posterior endoscopic excision may be an option for treating intraosseous lesions of the os trigonum because of good visualization, satisfactory excision, and rapid recovery time.
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http://dx.doi.org/10.3928/01477447-20111122-32DOI Listing
January 2012

Proanthocyanidin prevents myocardial ischemic injury in adult rats.

Med Sci Monit 2011 Nov;17(11):BR326-331

Department of Cardiovascular Surgery, Gulhane Military Medical School, Ankara, Turkey.

Background: Proanthocyanidin is a bioflavonoid known to have protective effect against oxidative injury. We investigated the cardioprotective effect of proanthocyanidin.

Material/methods: Thirty-two Rattus Norvegicus rats were categorized equally as the control group (CG), proanthocyanidin group (PCG), ischemia group (IG) and proanthocyanidin-treated group (PCT). Rats in CG and IG were fed standard rat food and PCG and PCT were fed standard rat food plus proanthocyanidin (100 mg/kg/day twice a day by oral gavage) for 3 weeks. In CG and PCG the myocardial samples were prepared immediately, and in IG and PCT hearts were placed in transport solution and kept at 4°C for 5 hours, then prepared for evaluation. Malondialdehyde (MDA) level, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were measured.

Results: MDA levels were significantly higher in IG and PCT than in CG and PCG. The activity of SOD was significantly lower in IG and higher in PCG than in the other groups. The activity of GPx was significantly lower in IG than in the other groups. The activities of CAT were significantly lower in IG and PCT than in the other groups and were significantly lower in IG than PCT. Histopathologic evaluation revealed normal findings in CG and PCG. While ischemic injury was observed in IG, the content of muscle fibers was better preserved in PCT.

Conclusions: Proanthocyanidin may have a protective effect on myocardial ischemic injury.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539496PMC
http://dx.doi.org/10.12659/msm.882042DOI Listing
November 2011