Publications by authors named "Maureen Duflot"

3 Publications

  • Page 1 of 1

Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish: Digestion method and viability studies.

J Fish Dis 2021 Jul 21. Epub 2021 Jul 21.

ANSES, Laboratory for Food Safety, Boulogne-sur-Mer, France.

Some trematode metacercariae, including marine digeneans belonging to the genus Cryptocotyle, induce black spots in target tissues due to the attraction of fish host melanophores. To promote precise quantification of infection, the counting of black spots has to be confirmed by reliable quantification of metacercariae after tissue digestion. This process ensures the isolation of undamaged parasites for morphological and molecular identification. The aim of this work was to optimize the pepsin digestion protocol and to assess the duration of viability of Cryptocotyle metacercariae in fish post-mortem (pm). Four digestion protocols were compared by measuring the viability rate of metacercariae. The present study shows that the orbital digestion method was the least destructive for metacercariae and allowed better quantification of Cryptocotyle infection. Moreover, morphological identification seemed reliable up to 8 days pm for Cryptocotyle infection.
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http://dx.doi.org/10.1111/jfd.13495DOI Listing
July 2021

A review of molecular identification tools for the opisthorchioidea.

J Microbiol Methods 2021 Aug 1;187:106258. Epub 2021 Jun 1.

ANSES, Laboratory for Food Safety, F-62200 Boulogne-sur-Mer, France. Electronic address:

The superfamily Opisthorchioidea encompasses the families Cryptogonimidae, Opisthorchiidae and Heterophyidae. These parasites depend on the aquatic environment and include marine and freshwater species. Some species, such as Clonorchis sinensis and Opisthorchis viverrini, have a high impact on public health with millions of infected people worldwide and have thus been the object of many studies and tool developments. However, for many species, tools for identification and detection are scarce. Although morphological descriptions have been used and are still important, they are often not efficient on the immature stages of these parasites. Thus, during the past few decades, molecular approaches for parasite identification have become commonplace. These approaches are efficient, quick and reliable. Nonetheless, for some parasites of the superfamily Opisthorchioidea, reference genomic data are limited. This study reviews available genetic data and molecular tools for the identification and/or the detection of this superfamily. Molecular data on this superfamily are mostly based on mitochondrial and ribosomal gene sequence analyses, especially on the cytochrome c oxidase subunit 1 gene and internal transcribed spacer regions respectively.
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http://dx.doi.org/10.1016/j.mimet.2021.106258DOI Listing
August 2021

LF H NMR T relaxation rate as affected by water addition, NaCl and pH in fresh, frozen and cooked minced hake.

Food Chem 2019 Mar 23;277:229-237. Epub 2018 Oct 23.

Institute of Food Science, Technology and Nutrition, Consejo Superior de Investigaciones Científicas (ICTAN-CSIC), Calle José Antonio Novais 10, 28040 Madrid, Spain. Electronic address:

Low-Field Nuclear Magnetic Resonance of proton transverse relaxation signal (T) was monitored in hake (Merluccius merluccius) mince with different thermal histories (fresh, frozen, cooked) as affected by pH, water and NaCl addition, and it was related to water holding capacity (WHC). Modifications of T signals were found in terms of changes in relaxation times and relative abundance of the relaxation components. The relaxation rate of the major component (1/T) increased significantly upon frozen storage or pH increase, whereas water or NaCl addition had the opposite effect. WHC decreased with freezing or water addition and increased with NaCl or basic pH; thus, T and WHC best correlated when each factor was analysed separately. Linearity found for pH and protein concentration with 1/T was consistent with chemical exchange being responsible for these changes. The significance of these results for technological situations where compositional and biochemical changes are occurring is discussed.
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http://dx.doi.org/10.1016/j.foodchem.2018.10.106DOI Listing
March 2019