Publications by authors named "Masoud Parsania"

13 Publications

  • Page 1 of 1

Prevalence and phylogenetic analysis of HTLV-1 in blood donors in Golestan Province, in the Northeast of Iran.

J Virol Methods 2021 Jan 21;290:114073. Epub 2021 Jan 21.

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Electronic address:

The human T-lymphotropic virus type 1 (HTLV-1) can cause ATL or TSP. This study evaluates the prevalence of HTLV-1 infection in blood donors in Golestan province. The study was conducted among 4226 blood donors and ELISA test was performed for the initial HTLV-1 screening. Reactive samples were confirmed by Western blot and Electrochemiluminescence tests. Then recalling donors with reactive results was done and genomic DNA from the new sample was extracted and tested using the Nested PCR method and phylogenetic analysis was performed. At first, 8 samples were reactive with ELISA test and 4 samples were confirmed with western blot, Electrochemiluminescence and Nested PCR tests.The sequences of isolates was related to the HTLV-1 virus and subtype a (cosmopolitan) subgroup A.The prevalence of HTLV-1 virus in Golestan province was about 0.09 %.The genotype of virus isolates had a common ancestor with isolates of the Khorasan region.
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http://dx.doi.org/10.1016/j.jviromet.2021.114073DOI Listing
January 2021

Argon and Argon-Oxygen Plasma Surface Modification of Gelatin Nanofibers for Tissue Engineering Applications.

Membranes (Basel) 2021 Jan 2;11(1). Epub 2021 Jan 2.

Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran 19395/1495, Iran.

In the present study, we developed a novel approach for functionalization of gelatin nanofibers using the plasma method for tissue engineering applications. For this purpose, tannic acid-crosslinked gelatin nanofibers were fabricated with electrospinning, followed by treatment with argon and argon-oxygen plasmas in a vacuum chamber. Samples were evaluated by using scanning electron microscopy (SEM), atomic force microscopy (AFM), attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, contact angle (CA) and X-ray diffraction (XRD). The biological activity of plasma treated gelatin nanofibers were further investigated by using fibroblasts as cell models. SEM studies showed that the average diameter and the surface morphology of nanofibers did not change after plasma treatment. However, the mean surface roughness (RMS) of samples were increased due to plasma activation. ATR-FTIR spectroscopy demonstrated several new bands on plasma treated fibers related to the plasma ionization of nanofibers. The CA test results stated that the surface of nanofibers became completely hydrophilic after argon-oxygen plasma treatment. Finally, increasing the polarity of crosslinked gelatin after plasma treatment resulted in an increase of the number of fibroblast cells. Overall, results expressed that our developed method could open new insights into the application of the plasma process for functionalization of biomedical scaffolds. Moreover, the cooperative interplay between gelatin biomaterials and argon/argon-oxygen plasmas discovered a key composition showing promising biocompatibility towards biological cells. Therefore, we strongly recommend plasma surface modification of nanofiber scaffolds as a pretreatment process for tissue engineering applications.
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http://dx.doi.org/10.3390/membranes11010031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823286PMC
January 2021

Association of TLR3 single nucleotide polymorphisms with susceptibility to HTLV-1 infection in Iranian asymptomatic blood donors.

Rev Soc Bras Med Trop 2020 22;53:e20200026. Epub 2020 Jun 22.

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.

Introduction: The human T-lymphotropic virus type 1 (HTLV-1) has a single-stranded RNA genome and expresses specific proteins that have oncogenic potential. Approximately 15 to 20 million people worldwide have been infected by this virus. Changes in protein or gene expression are the effects of single nucleotide polymorphisms (SNPs) within the Toll-like receptor 3 (TLR3) gene. The function and efficacy of signal transduction also lead to modified immune responses. The present study aimed to investigate the association of SNPs within TLR3 (rs3775291 and rs3775296) with susceptibility to HTLV-1 infection in Iranian asymptomatic blood donors.

Methods: This study was performed on 100 HTLV-1-infected asymptomatic blood donors and 118 healthy blood donors. Genomic DNA from all participants was purified and then amplified using specific PCR primers. SNPs within TLR3 were evaluated using the restriction fragmentation length polymorphism technique, and the results were analyzed using SPSS software (version 22).

Results: The frequencies of the TLR3 (rs3775296) CC, CA, AA genotypes were 70%, 24%, and 6% in the patient group, and 50.8%, 44.9%, and 4.2% in the control group, respectively. There was a significant difference in the frequency distribution of TLR3 (rs3775296) genotypes and alleles, but not in the frequency distribution of TLR3 (rs3775291) genotypes between the patient and control groups.

Conclusions: The TLR3 SNP rs3775296 was significantly associated with HTLV-1 infection and may be a protective factor against this viral infection.
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http://dx.doi.org/10.1590/0037-8682-0026-2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310369PMC
June 2020

Antiviral screening of four plant extracts against acyclovir resistant herpes simplex virus type-1.

Pak J Pharm Sci 2017 Jul;30(4(Suppl.)):1407-1411

Medical genomics research center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.

Herpes simplex virus type 1 (HSV-1) causes serious infections particularly in immunocompromised patients. Methanolic extract of four plants were evaluated for their anti-viral effects against acyclovir resistant HSV-1 in HeLa cell line. The 50% cytotoxic concentration (CC50) as well as the effective minimal cytotoxic concentration of each plant extract were evaluated by MTT assay. Antiviral effects of the plant extracts on HSV-1 were examined at different concentrations of the extract. The effective minimal cytotoxic concentration was evaluated at different times of virus replication after infection. Virus titration was assessed by tissue culture infectious dose 50 (TCID50) method. Among the 4 plant extracts evaluated only Mentha pulegium L. extract was shown to exert the highest antiviral activity, with selectivity index (SI) 10.25. Direct treatment of HSV-1 with Mentha pulegium L. extract resulted in 1.7 log10 TCID50 reduction in virus titers after one hour. The highest reduction in HSV-1 infectivity was obtained 1 hour after the infection of the cells with virus resulting in 2.1 log10 TCID50 reduction as compared to the control. The antiviral effects of Mentha pulegium L. extract on HSV-1 after virus infection was more remarkable than the virucidal activity.
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July 2017

Detection of Human Metapneumovirus and Respiratory Syncytial Virus by Real-Time Polymerase Chain Reaction Among Hospitalized Young Children in Iran.

Jundishapur J Microbiol 2016 Mar 15;9(3):e32974. Epub 2016 Mar 15.

Department of Pediatrics, Iran University of Medical Sciences, Tehran, IR Iran.

Background: Acute respiratory infection plays an important role in hospitalization of children in developing countries; detection of viral causes in such infections is very important. The respiratory syncytial virus (RSV) is the most common etiological agent of viral lower respiratory tract infection in children, and human metapneumovirus (hMPV) is associated with both upper and lower respiratory tract infections among infants and children.

Objectives: This study evaluated the frequency and seasonal prevalence of hMPV and RSV in hospitalized children under the age of five, who were admitted to Aliasghar children's hospital of Iran University of Medical Sciences from March 2010 until March 2013.

Patients And Methods: Nasopharyngeal or throat swabs from 158 hospitalized children with fever and respiratory distress were evaluated for RSV and hMPV RNA by the real-time polymerase chain reaction (PCR) method.

Results: Among the 158 children evaluated in this study, 49 individuals (31.1%) had RSV infection while nine individuals (5.7%) had hMPV infection. Five (55.5%) of the hMPV-infected children were male while four (44.5%) were female and 27 (55.2%) of the RSV-infected patients were females and 22 (44.8%) were males. The RSV infections were detected in mainly < one year old children and hMPV infections were detected mainly in > one year old children. Both RSV and hMPV infections had occurred mainly during winter and spring seasons.

Conclusions: Respiratory syncytial virus was the major cause of acute respiratory infection in children under one-year of age while human metapneumovirus had a low prevalence in this group. The seasonal occurrence of both viruses was the same.
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http://dx.doi.org/10.5812/jjm.32974DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877467PMC
March 2016

An endogenous immune adjuvant released by necrotic cells for enhancement of DNA vaccine potency.

Iran J Immunol 2012 Dec;9(4):215-25

Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran, e-mail:

Background: Improving vaccine potency in the induction of a strong cell-mediated cytotoxicity can enhance the efficacy of vaccines. Necrotic cells and the supernatant of necrotic tumor cells are attractive adjuvants, on account of their ability to recruit antigen-presenting cells to the site of antigen synthesis as well as its ability to stimulate the maturation of dendritic cells.

Objective: To evaluate the utility of supernatant of necrotic tumor cells as a DNA vaccine adjuvant in a murine model.

Method: The supernatant of EL4 necrotic cells was co-administered with a DNA vaccine expressing the glycoprotein B of Herpes simplex virus-1 as an antigen model under the control of Cytomegalovirus promoter. C57BL/6 mice were vaccinated three times at two weeks intervals with glycoprotein B DNA vaccine and supernatant of necrotic EL4 cells. Five days after the last immunization, cell cytotoxicity, IFN-γ and IL-4 were evaluated.

Results: The obtained data showed that the production of IFN-γ from the splenocytes after antigenic stimulation in the presence of the supernatant of necrotic EL4 cells was significantly higher than the other groups (p<0.002). The flow cytometry results showed a significant increase in the apoptosis/necrosis of EL4 cells in the mice immunized with DNA vaccine and supernatant of necrotic EL4 cells comparing to the other groups (p<0.001).

Conclusion: The supernatant of necrotic cells contains adjuvant properties that can be considered as a candidate for tumor vaccination.
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http://dx.doi.org/IJIv9i4A1DOI Listing
December 2012

Effect of LIGHT adjuvant on kinetics of T-cell responses induced by HSV-1 DNA immunization.

Iran J Immunol 2011 Jun;8(2):76-84

Department of Virology, Tarbiat Modares University, Tehran, Iran.

Background: Studies on efficacy of various vaccines that prevent or reduce the primary and recurrent HSV-1 infection have demonstrated the importance of cellular immunity for protection against the infection. We previously used DNA vaccination to induce cellular immunity against HSV-1 infection in mice.

Objective: The aim of our study was to evaluate the effect of LIGHT; a member of TNF super family, on the kinetic of CTL response induced by HSV-1 glycoprotein B based DNA vaccine.

Methods: Using a granzyme B ELISA for detection and analysis of CD8+ T cells, CTL activity was determined in the spleen of BALB/c mice at various time points after primary and booster dose of vaccination. The kinetics of CTL response to primary and secondary HSV-1 infection and DNA vaccination were compared to those induced by DNA vaccination in combination with LIGHT adjuvant in the present study.

Results: In primary and secondary immunization, the CTL activity in the HSV injected group peaked 7 days and 12 hours post immunization, respectively. After 5 days, LIGHT could neither accelerate the CTL response compared to DNA vaccination alone nor could enhance the CTL activity in the primary and the first peak of memory response, the amount of granzyme B induced by the LIGHT containing vaccine was significantly higher than that induced by the vaccine without the adjuvant.

Conclusion: Although LIGHT enhances the cellular response in the booster dose of vaccination, it does not accelerate the CTL response.
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http://dx.doi.org/IJIv8i2A2DOI Listing
June 2011

Full length antigen priming enhances the CTL epitope-based DNA vaccine efficacy.

Cell Immunol 2011 19;268(1):4-8. Epub 2011 Jan 19.

Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

Although CD8+ cytotoxic T lymphocyte (CTL) epitope-based DNA vaccination is valuable experience on vaccine research but many attempts are still continued to achieve acceptable protective response. To study the role of full length antigen in CTL epitope immunization, we evaluated cellular immunity of diverse patterns of complete Herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) and the immunodominant CTL epitope (498-505) DNA injection in C57BL/6 mice. Optimal immune response was observed in the group immunized with the full length of gB in the first injection and CTL epitope in the second and third vaccination as assessed by lymphocyte proliferation assay (MTT), cytokine assay (ELISA) and CTL assay. B cell and spatially CD4+ T cell epitopes in full length protein might be important for appropriate priming of CTL immune response. These findings may have important implication for the improvement of CTL epitope based DNA vaccine against HSV and other pathogens.
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http://dx.doi.org/10.1016/j.cellimm.2011.01.003DOI Listing
May 2011

Impact of timing strategy of LIGHT, a new TNF superfamily on immune platform induced by HSV-1 gB DNA vaccine.

Cytokine 2010 Apr 22;50(1):99-103. Epub 2010 Jan 22.

Department of Virology, Tarbiat Modares University, Tehran, Iran.

Although the role of various cytokines on stimulating the immune responses is characterized well, the importance of LIGHT, a member of TNF superfamily, is less clear. In the current study, we administrated LIGHT expression plasmid as an adjuvant to HSV-1 gB DNA vaccine. HSV-1 gB DNA can elicit vigorous humoral and cell mediated immunity in BALB/c mice. LIGHT could potentiate the proliferation of T lymphocytes and induction of T CD8(+) cells performing by measuring Granzyme B, a specific marker of CMI immunity and virus neutralization antibody titer. In this study, timing effect of cytokine administration on the resultant immune pattern was evaluated in three different timing groups. The group received LIGHT 3 days before DNA vaccine, demonstrated significant increase in cell mediated immunity. So, utilization of an adjuvant to DNA vaccine can significantly influences the induced immune response consequently and this phenomenon could be important to obtain the optimal response in DNA vaccine strategy. Given the growing use of plasmid-based immune adjuvants to improve the immunogenicity and efficacy of DNA vaccines, these findings support the need for further detailed study of this class of agent.
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http://dx.doi.org/10.1016/j.cyto.2009.12.012DOI Listing
April 2010

Evaluation of apoptotic and anti-apoptotic genes on efficacy of DNA vaccine encoding glycoprotein B of Herpes Simplex Virus type 1.

Immunol Lett 2010 Feb 21;128(2):137-42. Epub 2009 Dec 21.

Dept of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Islamic Republic of Iran.

Many approaches have so far been tried to enhance the immunogenicity of DNA vaccine. These include the use of various factors that induce apoptosis or anti-apoptosis effects when co-delivered with DNA vaccine. In the present study, the effects of pro-apoptotic Bax encoding plasmid (pBax) and anti-apoptotic Bcl-X(L) encoding plasmid (pBcl-xl), intradermally co-injected with glycoprotein B (gB) of Herpes Simplex Virus (HSV)-1 encoding plasmid (pgB) into the C57BL/6 mice were evaluated. Immune responses of the mice to the antigen were assessed by antibody assay, lymphoproliferative responses as well as cytokine and cytotoxic T-lymphocyte (CTL) assay. Analysis of the humoral and cellular responses showed that the mice immunized with pBax and pgB induced higher levels of antibody and Interleukin-4 as well as stronger lymphocyte proliferative responses and cytotoxic activity compared to those mice received pgB alone. pBcl-xl when intradermally co-injected with pgB showed no significant enhancement in immune responses comparing to pgB.
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http://dx.doi.org/10.1016/j.imlet.2009.12.014DOI Listing
February 2010

Kinetics of primary and memory cytotoxic T lymphocyte responses to herpes simplex virus 1 infection: granzyme B mediated CTL activity.

Iran J Immunol 2009 Mar;6(1):22-7

Department of Virology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Background: Herpes simplex virus type 1 is one of the most common viruses among human population. Studies demonstrate the essential role of cell mediated immunity, especially CD8+ T cells, in prevention and clearance of HSV1.

Objective: It is of great importance to improve our knowledge about the kinetics of CTL responses to primary and secondary HSV-1 infection.

Methods: Using a sensitive technique for detection and analysis of CD8+ T cells, granzyme B ELISA, the CTL activity in the spleens of Balb/c mice at various time points after intraperitoneal administration of HSV1 (strain KOS) in primary and secondary infections were determined.

Results: During acute HSV-1 infection, virus specific cytotoxic T cells were detected at day 5 post virus inoculation and peaked at day 7. Six hours after secondary infection the activity of memory CD8+ T cells was detected and peaked at 12 hours post infection.

Conclusion: The peak of CTL activity was found to be day 7 post infection in primary HSV-1 infections which decreased with time. In secondary infections, the activity of CTLs reached the highest level at 12 hours post infection.
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http://dx.doi.org/IJIv6i1A3DOI Listing
March 2009

Naloxone, an opioid receptor antagonist, enhances induction of protective immunity against HSV-1 infection in BALB/c mice.

Microb Pathog 2007 Nov-Dec;43(5-6):217-23. Epub 2007 Jul 31.

Department of Virology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

The immunomodulatory effects of exogenous opioids on induction of acquired immunity during microbial infection are now well known; however, our knowledge about the relationship between endogenous opioid response and microbial infections is rudimentary. Here, we report the effect of administration of Naloxone (NLX), an opioid receptor antagonist, on induction of acquired immunity during primary herpes simplex virus type 1 (HSV-1) infection. BALB/c mice received NLX, twice daily, 2 h before infection with HSV-1 until 7 days after infection. Cell-mediated immunity was assessed by evaluating lymphocyte proliferation, interferon-gamma (IFN-gamma) production, delayed type hypersensitivity (DTH) and mortality rate after acute HSV-1 challenge. The findings showed that a higher level of cell-mediated immunity was induced in the NLX-treated animals compared to the control group after induction of HSV-1 infection. However, the data indicate similar neutralizing antibody production in NLX-treated animals and control animals. This observation and further studies in this field may lead to the use of NLX as an adjuvant for designing microbial vaccines and adjunctive therapy of viral infections.
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http://dx.doi.org/10.1016/j.micpath.2007.06.001DOI Listing
January 2008

Evaluation of gamma-interferon kinetics in HSV-1 infected mice in different days post infection (in vivo) and post re-stimulation (in vitro).

Comp Immunol Microbiol Infect Dis 2007 Jan 13;30(1):1-9. Epub 2006 Nov 13.

Department of Virology, School of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-111, Tehran, Iran.

Gamma interferon (IFN-gamma) is among the most important immune factors for limiting of herpes simplex virus (HSV) infections. However, our knowledge about the kinetics of IFN-gamma production after HSV infection is limited. The present study examines the kinetics of IFN-gamma expression following secondary infection with HSV-1. Using semiquantitative RT-PCR assay, the expression of IFN-gamma in spleen lymphocytes was significantly detected on 14 days but not 7 days after intraperitoneal inoculation of HSV-1, while ELISA detected IFN-gamma on both days. At various hours after in vitro re-stimulation of spleen cells, RT-PCR showed a decreasing pattern of mRNA transcripts, whereas, ELISA assayed an increasing amount of secreted protein through the experiment. Despite the contrast results of ELISA and RT-PCR, regarding the short half-life of mRNA, the data are in correlation with each other and need to interpret.
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http://dx.doi.org/10.1016/j.cimid.2006.09.001DOI Listing
January 2007