Publications by authors named "Masoud Beigi Boroujeni"

2 Publications

  • Page 1 of 1

Effect of Selenium on Expression of Apoptosis-Related Genes in Cryomedia of Mice Ovary after Vitrification.

Biomed Res Int 2020 23;2020:5389731. Epub 2020 Sep 23.

Department of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran.

Introduction: Freezing of ovarian tissue is used for preservation of fertility. The freezing-thawing process is accompanied by oxidative stress and induction of apoptosis. Apoptosis is a complex process that has been studied in animal models. The present study was aimed at investigating the effect of selenium on suppression of apoptosis during vitrification-thawing process of mice ovary via studying expression of apoptosis-related genes, and also, we aimed to design statistical models for the roles of single genes and gene-gene interactions in suppression of apoptosis.

Methods: A total of 10 right ovary samples from 10 mice were randomly divided into two groups of selenium treatment (at dose 5 g/ml sodium selenite, through adding to the media) and control group. Vitrification-thawing process was done according to the existed protocols. Real-time PCR was used for gene expression study. The apoptosis gene profile included , , , and . General linear model was applied to study single gene associations and gene-gene interactions.

Results: From the studied genes, showed a significant downregulation in the selenium group in comparison to the control group (∆∆CT = 1.96; = 0.013; relative expression (RE) = 0.28). showed a significant upregulation in the selenium group in comparison to the control group (∆∆CT = -2.49; < 0.001; RE = 3.49). No significant result was found for other genes. According to the multiple models, showed a protective single gene association (beta = -0.33; = 0.032), and ∗ interaction was significantly positive (beta = 0.19; = 0.036).

Conclusion: Addition of selenium to cryomedia of vitrification-thawing process could reduce the apoptosis induced by freezing-thawing stress in mice ovary via downregulation of and upregulation of at transcription level. Multivariable statistical models should be performed in future researches to study biological systems.
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May 2021

Ultrastructural changes of corpus luteum after ovarian stimulation at implantation period.

Iran Biomed J 2012 ;16(1):33-7

Dept. of Biology, School of Basic Sciences, Payame Noor University of Tehran, Tehran, Iran.

Background: To achieve multiple oocytes for in vitro fertilization, ovulation induction is induced by gonadotropins; however, it has several effects on oocytes and embryo quality and endometrium receptivity. The aim of this study was to assess ultrastructural changes of corpus luteum after ovarian induction using human menopausal gonadotropin (HMG) and human chorionic gonadotropin (HCG) during luteal phase at implantation period.

Methods: Female NMRI mice (6-8 weeks) were divided into control and stimulated groups. In the control group, the mice were rendered pseudopregnant and in the ovarian induction group, the mice were rendered pseudopregnant after the ovarian induction. The samples were obtained from the ovary in each group at the same time during luteal phase at implantation period. Ultrastructural changes were assessed using electron microscopy study.

Results: Our results displayed some identifiable changes in ultrastructure of corpus luteum in ovarian induction group. These changes included enhancement of the apoptosis and intercellular space, whereas the angiogenesis was decreased. The findings indicated a decline in organelle density in the cytoplasm of ovarian induction, such as mitochondria, endoplasmic reticulum and polyribosome. Furthermore, chromatin condensation of nuclei was observed in some cells.

Conclusion: The ovarian induction using HMG and HCG resulted in some ultrastructural changes on the corpus luteum at implantation period, which could affect on the pregnancy rate.
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September 2012