Publications by authors named "Mary Carmen Reyes Zamora"

5 Publications

  • Page 1 of 1

Effect of Biomodulina-T® and VA-MENGOC-BC® on lymphocyte subpopulations in older adults.

Exp Gerontol 2021 10 26;153:111497. Epub 2021 Jul 26.

Institute of Hematology and Immunology, Immunochemistry-Immunology Department, 19th St, between 8th and 10th St, Vedado, 10400 Plaza de la Revolución, Havana, Cuba. Electronic address:

Introduction: The elderly population suffers from the natural process called immunosenescence, which may be related to the high mortality rates it has against the SARS-CoV2 virus, which is why therapies that improve the immune status are required. The combined treatment of the VA-MENGOC-BC® (V-BC) vaccine and the Biomodulina T® (BT) drug could achieve this purpose. This treatment could immunomodulate both the innate and adaptive branches of the immune system simultaneously.

Objective: To determine the effect of BT and V-BC on the immunomodulation of lymphocyte subpopulations in older adults.

Methods: Our study was carried out in 30 apparently healthy Cuban adults over 65 years of age. The study included three groups of 10 subjects per treatment: a combination of both and the monotherapies. Before and 7 days after treatment, 2 mL of peripheral blood was drawn from each subject. Multiparametric flow cytometry was used to identify lymphocyte subpopulations. For the comparison between the groups, point estimates and the confidence intervals of the Odds Ratio were made.

Results: We found that subpopulations of B lymphocytes and natural cytotoxic T (NKT) cells increased only with the administration of BT. Additionally, combination treatments and V-BC did not generate statistically significant immunomodulatory changes in any of the studied lymphocyte subpopulations.

Conclusions: BT presented an immunoenhancing effect on the B and NKT lymphocyte subpopulations of older adults. The three-dose treatment scheme a novel and specific treatment strategy for this formulation. We also were verified that the combined application of V-BC and BT did not have the expected benefits. All these findings suggest that BT administration is a promising approach for immune restoration and to offering protection in elderly patients against COVID-19.
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http://dx.doi.org/10.1016/j.exger.2021.111497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310781PMC
October 2021

Immunophenotypic characterization of double positive T lymphocytes in Cuban older adults.

Exp Gerontol 2021 09 10;152:111450. Epub 2021 Jun 10.

Subdirection for Medical Assistance, Longevity, Anging and Health Research Center (CITED), Vedado, Havana, Cuba. Electronic address:

Introduction: Immunosenescence is the functional deterioration of the immune system during natural aging that mainly affects T lymphocytes, within which is the subpopulation of peripheral double positive T lymphocytes that are immunomodulators of the immune response. They are increased in older adults and play a fundamental role in the immunopathogenesis of autoimmune diseases, viral infections and neoplasms.

Objective: To characterize the immunophenotype of peripheral double positive T lymphocytes in Cuban older adults.

Methods: A cross-sectional study was carried out in 30 older adults residing in Cuba. Peripheral blood double positive T lymphocytes were quantified by flow cytometry. The reading was done on a Beckman Coulter Gallios flow cytometer. A Beckman Coulter Gallios flow cytometer was used for data analysis. Percentage values, median and standard deviation were used.

Results: There was a predominance of women who represented 70%. No older adults with low values of double positive T lymphocytes were reported. Women with high percentage and absolute values of DPT lymphocytes predominated in relation to men. Among the high percentage values and the absolute value of DPT lymphocytes, the group ≥80 years prevailed.

Conclusions: Most of the older adults who exhibited high percentage and absolute values of DPT lymphocytes had some associated comorbidity, an idea that suggests that CD4 + CD8+ T cells seem to play an important role in peripheral sites as powerful immunosuppressants or as cells with high cytotoxic potential.
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http://dx.doi.org/10.1016/j.exger.2021.111450DOI Listing
September 2021

Is peanut causing food allergy in Cuba? Preliminary assessment of allergic sensitization and IgE specificity profile to peanut allergens in Cuban allergic patients.

World Allergy Organ J 2017 11;10(1):26. Epub 2017 Jul 11.

Institute of Pharmacy and Foods, Havana University, Havana, Cuba.

Background: Peanut allergy is increasing at an alarming pace in developed countries. Peanut () is a common food in Cuba. Nevertheless, reported values of sensitization and symptom severity are usually low. As our objective, we carried out an evaluation of allergic sensitivity to perform an assessment of allergic sensitization and IgE specificity profile to peanut allergens in Cuban allergic patients.

Methods: The Skin Prick Test (SPT) was performed for each patient, using two glycerinated allergenic extracts, prepared from raw or roasted peanuts. Overall, 316 food allergic patients (159 adults and 157 children) attending allergy services at four hospitals in Havana were included, as well as 303 adult non- allergic volunteers. The IgE binding profile of 26 selected SPT positive patients was further analyzed by immunoblotting.

Results: The prevalence of sensitization to peanut was 4.6% in general adult population, whereas in adult food-allergic patients it was 18.6%. Prevalence rates were even greater in food allergic children achieving 25.8%. Sensitization frequencies were apparently greater for roasted, as compared to raw peanuts, although the difference was not significant (> 0.05, Mc Nemar's). IgE binding was shown mostly by the 15 and 17 kDa bands, tentatively identified as the major allergens Ara h 2 and Ara h 6. The IgG4 binding profile was similar to IgE, although with more prominence of the bands at 37 and 28 KDa, corresponding to an Ara h 3 fragment and Peanut Agglutinin.

Discussion: The study estimated a relatively high prevalence of peanut sensitization in population. Data reported here suggest that IgE sensitization in Cuban patients is focused mostly on MW bands corresponding to the major allergens Ara h 6 and Ara h 2.

Conclusions: Sensitization to peanut allergen is indeed relatively frequent in Cuba. The IgE profile is congruent to a sensitization pattern by ingestion of roasted peanuts and is directed to well-known major allergens.
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http://dx.doi.org/10.1186/s40413-017-0156-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5504782PMC
July 2017

[Sensitivity and specificity of prick skin test with two concentrations of standardized extract of Culex quinquefasciatus in allergic children].

Rev Alerg Mex 2016 Jan-Mar;63(1):11-9

Departamento de Alergenos, Centro Nacional de Biopreparados. Dirección de Investigaciones, Facultad de Ciencias Médicas General Calixto García Íñiguez, La Habana, Cuba.

Background: Diagnostic options for immune reactions to mosquito bites are limited. In Cuba, IgE-mediated reactions are frequently related to Culex quinquefasciatus bite.

Objective: To determine the sensitivity and specificity of skin prick test with two doses of standardized extract in nitrogen protein units (PNU of Culex quinquefasciatus (BIOCEN, Cuba).

Material And Method: An analytical study was conducted on 100 children between 2 and 15 years old. Fifty atopic patients with a history of allergy to mosquito bite and positive specific serum IgE Culex quinquefasciatus and fifty atopic patients without a history of allergy to mosquito bite and negative specific serum IgE to Culex quinquefasciatus. Skin prick tests (SPT) were performed by duplicates on the forearms of the patients. Investigated doses were 100 PNU/mL and 10 PNU/mL.

Results: SPT with the highest concentration obtained a mean wheal size of 22.09 mm2 and for lower doses of 8.09 mm2, a statistically significant difference (p=0.001, Student's t test). Positive skin test correlated in 100% of patients with the presence of specific IgE. Testing with both doses showed a 94% of specificity and 88% of sensitivity.

Conclusion: The diagnostic accuracy of SPT using both doses of standardized extract was similar, which justifies its use for diagnosis of sensitization to Culex quinquefasciatus in patients with symptoms of allergy to mosquito bite.
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http://dx.doi.org/10.29262/ram.v63i1.114DOI Listing
October 2018

[Sensitization to three house dust mites in a children allergic population of cuba].

Rev Alerg Mex 2012 Jul-Sep;59(3):148-54

Departamento de Alergenos BioCen, Mayabeque, Cuba.

Background: Allergic diseases constitute a worldwide health problem and mites are among the main etiological agents.

Objective: To characterize the sensitization to the mites Dermatophagoides pteronyssinus, Dermatophagoides siboney, and Blomia tropicalis, by skin tests in a children allergic sample from Cuba.

Methods: This is a descriptive transversal investigation that included 103 allergic children, with ages ranging from 3 to 15 years, who underwent skin prick tests with D. pteronyssinus, D. siboney, and B. tropicalis allergenic extracts. For the analysis of the results, sex, place of residence and degree of exposure to house dust were considered.

Results: The greater sensitization was to D. pteronyssinus (36,9%), followed by D. siboney (35%) and B. tropicalis (33%). The greater geometric mean of the wheal diameter was 5,14 mm for D. pteronyssinus (IC95%: 4.52-5.76 mm); 4.82 mm for D. siboney (IC95%: 4.22-5.43 mm) and 4.25 mm for B. tropicalis (IC95%: 3.93-4.58 mm). Fifty six children (54.3%) were not sensitive to none of the three mites. In 45.7% we found sensitization at least to one mite, and 21.4% were sensitive to the three mites. There were no significant differences (p >0.05) in the sensitization according to sex, place of residence and degree of exposure to house dust. The percentage of sensitive patients with rhinitis plus asthma was more significant (p < 0.001), and in them, the geometric mean of the wheal diameter was significant for D. pteronyssinus (p=0.028) and D. siboney (p=0. 027).

Conclusions: A third of our studied child population, residing in a non-industrialized tropical area, presented sensitization to one or some of the three tested mites. There was a different pattern of sensitization with mites in relation to gender. Patients with asthma plus rhinitis showed more sensitization concerning the number and intensity of the skin tests.
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April 2016
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