Publications by authors named "Martina Ustulin"

5 Publications

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A case of pericarditis caused by Mycoplasma hyorhinis in a weaned piglet.

Porcine Health Manag 2021 Apr 12;7(1):32. Epub 2021 Apr 12.

Peripheral Diagnostic Laboratory of Pordenone, Istituto Zooprofilattico Sperimentale delle Venezie, Cordenons, PN, Italy.

Background: Mycoplasma hyorhinis (M. hyorhinis) is a bacterium commonly found in the upper respiratory tract of healthy pigs and an agent of polyserositis and polyarthritis. Moreover, it can carry antibiotic resistance genes (Wu et al, Vet. Microbiol. 76: 25-30, 2000). Economic losses caused by M. hyorhinis can be reduced by antibiotic therapy, however, isolation and antimicrobic susceptibility profile are rarely performed.

Case Presentation: The present report describes a case of pericarditis caused by M. hyorhinis in a weaned piglet with respiratory symptoms and reduced growth performance. At post mortem examination, the main macroscopic finding was a severe fibrinous pericarditis and M. hyorhins was the only agent isolated from the pericardial fluid. In this strain, Minimum Inhibitory Concentration (MIC) determination revealed resistance to various antimicrobial molecules such as erythromycin, tylosin and tilmicosin.

Conclusion: This paper highlights the importance of including M. hyorhins in the differential diagnosis of polyserositis in swine. Moreover, due the possible presence of multidrug resistance, the determination of antimicrobial susceptibility pattern should be performed on a regular basis.
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http://dx.doi.org/10.1186/s40813-021-00211-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040207PMC
April 2021

Environmental Exposure of Wild Carnivores to Zoonotic Pathogens: Infection in the First Free Living Wolf ( Linnaeus, 1758) Found Dead in the Friuli Venezia Giulia Region.

Int J Environ Res Public Health 2021 03 3;18(5). Epub 2021 Mar 3.

Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università, 35020 Legnaro, Italy.

Leptospirosis is a worldwide-spread zoonosis causing disease and death in dogs and in humans. A Leptospiral infection has been recorded in several wild carnivore species in Europe, but tissue pathological changes were not commonly described. The Grey wolf () has been expanding its distribution range in north-eastern Italy during the last decade. A young wolf, representing the first individual handled in the region, was found road-killed and then submitted to necropsy. Pathological changes included erosive lesions of gingival mucosa, mild liver enlargement, and multifocal degenerative-necrotic areas along with hyperemic reactive lesions; multifocal interstitial nephritis and multifocal lung hemorrhages were observed. A Polymerase Chain Reaction (PCR) able to detect pathogenic species of performed on a kidney sample was positive. Serological reactions for serogroup Gryppotyphosa (1:6400), Pomona (1:800), and Icterohaemorrhagiae (1:200) were evidenced by MAT. Genotyping by Multilocus Sequence Typing (MLST) performed on detected characterized it as belonging to Sequence Type (ST) 117, which refers to , serogroup Pomona, serovar Mozdok. Regardless of the role of infection as an eventual predisposing factor to the road killing of this wolf, to the best of the authors' knowledge, this is the first report of -induced pathology in a wolf in Europe. Surveys on infection in free-ranging wildlife species should be pursued in order to achieve further epidemiological knowledge on the circulation of the strain.
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http://dx.doi.org/10.3390/ijerph18052512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967383PMC
March 2021

Identification of two divergent swine Noroviruses detected at the slaughterhouse in North East Italy.

Porcine Health Manag 2020 16;6. Epub 2020 Apr 16.

3Diagnostic Virology Laboratory, Department of Animal Health, Istituto Zooprofilattico Sperimentale Delle Venezie, Legnaro, Padua, Italy.

Norovirus (NoV) has emerged as one of the major causative agents of non-bacterial, food- and water-borne gastroenteritis in humans, with the main genogroup involved in human outbreaks (GII), which has been detected worldwide in different animal species including swine. A four-month investigation at the slaughterhouse aiming to examine the presence of NoV in the swine in North-Eastern Italy, enabled the detection of two divergent Noroviruses (NoVs) (GII.P11) in two swine farms. This represents the first study in the swine population of North-Eastern Italy, which has paved the way for future integrated virological and epidemiological investigations on swine NoVs.
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http://dx.doi.org/10.1186/s40813-020-00147-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160966PMC
April 2020

Phenotypic features and phylogenetic background of extraintestinal hemolytic Escherichia coli responsible of mortality in puppies.

Vet Microbiol 2015 Aug 14;179(1-2):126-30. Epub 2015 Mar 14.

Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro, Italy. Electronic address:

A 10-day-old litter of five puppies of Bracco Italiano dog breed showed weakness and diarrhea and, 2 days later, four of them died. At the same time, the bitch showed high hyperthermia (40 °C) and endometritis. The necropsy of a puppy revealed a severe lobar pneumonia accompanied with a bilateral nephrosis. No gross lesions were detected in other organs. Histopathology of the lung revealed severe multifocal fibrino-suppurative necrotizing bronchiolar-alveolitis associated with rod-shaped bacterial aggregates and diffuse interstitial lymphocytic infiltration. The kidney showed severe multifocal necrosis of the tubular epithelium and diffuse severe congestion of the parenchyma. A pure culture of hemolytic Escherichia coli carrying the Cnf-1 gene was identified, from both the puppy organs and bitch's milk. Moreover, phylo-typing assigned them to the phylogroup B2. Two weeks later, fecal samples from the bitch and the survived puppy were collected for a second microbiological analysis, identifying two hemolytic E. coli strains, Cnf positive and Cdt negative and Cnf and Cdt negative, respectively. Some E. coli pathogenic strains may cause enteric or extraintestinal disease. In dogs and cats, strains of extraintestinal pathogenic E. coli (ExPEC) produce specific virulent factors such as hemolysis and cytotoxin necrotizing factors (Cnf). In this episode, we hypothesize that the bitch's milk could be the main source of ExPEC infection causing high puppies mortality. The role of the bitch as a carrier could not be excluded: stressful conditions, such as pregnancy and delivery, would change the host-pathogen dynamics possibly increasing the release of the infectious burden.
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http://dx.doi.org/10.1016/j.vetmic.2015.03.004DOI Listing
August 2015

Genetic complexity and multiple infections with more Parvovirus species in naturally infected cats.

Vet Res 2011 Mar 2;42:43. Epub 2011 Mar 2.

Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Italy.

Parvoviruses of carnivores include three closely related autonomous parvoviruses: canine parvovirus (CPV), feline panleukopenia virus (FPV) and mink enteritis virus (MEV). These viruses cause a variety of serious diseases, especially in young patients, since they have a remarkable predilection for replication in rapidly dividing cells. FPV is not the only parvovirus species which infects cats; in addition to MEV, the new variants of canine parvovirus, CPV-2a, 2b and 2c have also penetrated the feline host-range, and they are able to infect and replicate in cats, causing diseases indistinguishable from feline panleukopenia. Furthermore, as cats are susceptible to both CPV-2 and FPV viruses, superinfection and co-infection with multiple parvovirus strains may occur, potentially facilitating recombination and high genetic heterogeneity. In the light of the importance of cats as a potential source of genetic diversity for parvoviruses and, since feline panleukopenia virus has re-emerged as a major cause of mortality in felines, the present study has explored the molecular characteristics of parvovirus strains circulating in cat populations. The most significant findings reported in this study were (a) the detection of mixed infection FPV/CPV with the presence of one parvovirus variant which is a true intermediate between FPV/CPV and (b) the quasispecies cloud size of one CPV sample variant 2c. In conclusion, this study provides new important results about the evolutionary dynamics of CPV infections in cats, showing that CPV has presumably started a new process of readaptation in feline hosts.
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http://dx.doi.org/10.1186/1297-9716-42-43DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3059301PMC
March 2011
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