Publications by authors named "Marta Bartosik"

3 Publications

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[Occurrence of Legionella in technological water and studies of the total number of bacteria and fungi in indoor air at workplaces where water aerosol is generated].

Med Pr 2014 ;65(3):325-34

Background: The aim of the study was to confirm the fact that technological water is a significant carrier of Legionella, a potential serious threat to the health of operators of mechanical devices generating contaminated water aerosol.

Material And Methods: Microbiological analyses of water and indoor air were conducted in 8 different production facilities involved in mechanical processing of glass. The study covered 81 samples of water collected from technical water systems. Isolation of Legionella and the determination of total number of microorganisms were processed according to PN-EN ISO 11731-2:2008E and PN-EN ISO 6222:2004P, respectively. Air samples were collected using air samplers and total numbers of bacteria and fungi were determined.

Results: The studies of process water, indicated the presence of Legionella in 27.2% of collected samples. These bacteria were present in both closed and open process water reservoirs at 10 cfu/100 ml to 2.9 x 10(4) cfu/100 ml. The count of other associated bacteria exceeded 10(3) cfu/ml. All strains isolated from Legionella-positive samples were identified as L. pneumophila SG 2-14. In 5 of 8 studied production facilities an increased total number of aerial bacteria and fungi was observed in samples collected in close vicinity of aerosol source.

Conclusions: To reduce the number of microorganisms in water it is required to introduce technological water quality monitoring and procedures for the cleaning and disinfecting of mechanical devices generating water aerosol.
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October 2014

Persistent colonization of 2 hospital water supplies by L. pneumophila strains through 7 years--sequence-based typing and serotyping as useful tools for a complex risk analysis.

Ann Agric Environ Med 2013 ;20(4):687-94

National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland.

Contamination with Legionella spp. of hot water system (HWS) in hospitals is a considerable problem and elimination of bacteria poses difficulties. Obligatory control of Legionella spp. in hospital HWS was implemented in Poland in 2008y. After that, Legionella spp. has been isolated repeatedly from HWS of the majority of hospitals. The aim of our study was to confirm the permanent colonization with Legionella spp. of 2 hospital HWSs based on the antigenic (serogroup/subgroups) and genetic properties (SBT, rtxA) of L.pneumophila strains isolated in 2004-2011. The dynamic of L.pneumophila population was also examined due to methods of disinfections applied during 7 years. Totally, 134 environmental samples were collected from two hospitals in 2004-2011 (118 from HWSs). During the study disinfection by chlorine dioxide was implemented in both hospitals, while thermal shock was added in the hospital A. Isolated L.pneumophila were serogrouped (105 strains) using Dresden MAb Panel, genotyped by sequence based typing (53) and by harboring of rtxA gene (58 isolates). Legionella spp. were still presented in both systems after 7 years. Exactly the same strains (ST1, ST87, ST114, ST992) were found in the hospital B. While changes of L.pneumophila population were observed in the hospital A: strains still occurred after 7 years (ST835 Sg6, ST114 Sg6); modified antigenic properties (ST835 - Sg12 vs. Sg6); eliminated or maybe not detected (ST81, ST838, ST959). Moreover, the majority of examined strains ST1 (Sg1, OLDA) harboured rtxA gene (hospital B). Our results and data in the EWGLI SBT base indicated higher risk of Legionella infection in the hospital B than A--because of heavy colonization with L.pneumophila ST1. The risk assessment of Legionella infection based only on technical parameters, extent of colonization/contamination level may be not completed. It should be supplemented with the additional examination: serotyping, genotyping and virulence testing of isolated strains.
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August 2014

[Respiratory symptoms among industrial workers exposed to water aerosol. A pilot study of process water and air microbial quality].

Med Pr 2013 ;64(1):47-55

Zakład Higieny Srodowiska, Narodowy Instytut Zdrowia Publicznego - Paistwowy Zaklad Higieny, Warszawa, Poland.

Background: The frequency of respiratory symptoms in workers exposed to water aerosol was evaluated along with the preliminary assessment of microbiological contamination of air and water used in glass processing plants.

Material And Methods: A questionnaire survey was conducted in 131 workers from 9 glass processing plants. Questions focused on working conditions, respiratory symptoms and smoking habits. A pilot study of air and water microbiological contamination in one glass processing plant was performed. Water samples were tested for Legionella in accordance with EN ISO 11731-2:2008 and for total colony count according to PN-EN ISO 6222:2004. Air samples were tested for total numbers of molds and mildews.

Results: During the year preceding the survey acute respiratory symptoms occurred in 28.2% of participants, while chronic symptoms were reported by 29% of respondents. Increased risks of cough and acute symptoms suggestive of pneumonia were found among the respondents working at a distance up to 20 m from the source of water aerosol compared to other workers (OR = 2.7), with no difference in the frequency of other symptoms. A microbiological analysis of water samples from selected glass plant revealed the presence of L. pneumophila, exceeding 1000 cfu/100 ml. The number of bacteria and fungi detected in air samples (above 1000 cfu/m3) suggested that water aerosol at workplaces can be one of the sources of the air microbial contamination.

Conclusions: The questionnaire survey revealed an increased risk of cough and acute symptoms suggestive of pneumonia in the group working at a shortest distance form the source of water aerosol.
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http://dx.doi.org/10.13075/mp.5893/2013/0006DOI Listing
June 2013
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