Publications by authors named "Martín H Fugassa"

9 Publications

  • Page 1 of 1

Ancient parasitic DNA reveals presence in Final Pleistocene of South America.

Parasitology 2019 09 3;146(10):1284-1288. Epub 2019 Jul 3.

Laboratorio de Parasitología de Sitios Arqueológicos, CONICET-UNMdP, Dean Funes 3250 (7600), Mar del Plata, Buenos Aires, Argentina.

Parasitological analysis of coprolites has allowed exploring ecological relationships in ancient times. Ancient DNA analysis contributes to the identification of coprolites and their parasites. Pleistocene mammalian carnivore coprolites were recovered from paleontological and archaeological site Peñas de las Trampas 1.1 in the southern Puna of Argentina. With the aim of exploring ancient ecological relationships, parasitological analysis was performed to one of them, dated to 16 573-17 002 calibrated years BP, with 95.4% probability. Parasite eggs attributed to Toxascaris sp. by morphological characters were isolated. DNA of coprolite and eggs was extracted to molecular identification. Ancient mitochondrial DNA analysis confirmed the zoological origin of the coprolite as Puma concolor and that of parasite eggs as Toxascaris leonina. This is the oldest molecular parasite record worldwide, and it supports the presence of this parasite since the Pleistocene in America. These findings have implications for the biogeographic history of parasites and for the natural history of the region.
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http://dx.doi.org/10.1017/S0031182019000787DOI Listing
September 2019

First mitochondrial and nuclear DNA sequences of Lamanema chavezi (Nematoda: Molineidae): Novel findings to improve its identification in feces from South American camelids.

Parasitol Int 2019 Feb 17;68(1):60-62. Epub 2018 Oct 17.

CONICET - Laboratorio de Parasitología de Sitios Arqueológicos, Universidad Nacional de Mar del Plata, Calle Funes 3350, Mar del Plata 7600, Buenos Aires, Argentina.

Lamanema chavezi (Family Molineidae) is a parasitic nematode of South American camelids (SACs). A few studies have reported this parasite in SACs, mainly in domestic camelid species (llama and alpaca). Parasite identification by means of copro-parasitological methods is non-invasive and allows performing epidemiological studies. However, egg misidentification and difficulty to culture third-stage larvae do not allow identifying nematodes to species level. In contrast, molecular tools allow identifying eggs of gastrointestinal nematodes more accurately. However, the little genomic information available in databases for some species prevents an accurate diagnosis. In the present work, L. chavezi females present in feces of llamas from northwestern Argentina were molecularly characterized to obtain genomic information and improve parasitological diagnosis of L. chavezi-like eggs present in guanaco feces from southeastern Argentina. An 833-bp fragment of nuclear ribosomal DNA (rDNA) and a 434-bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene from both L. chavezi females and eggs were amplified and sequenced. Comparison between sequences from females and eggs showed 99-99.6% identity to rDNA and 99.5-96.1% to the cox1 gene fragments, confirming egg morphological assignment. A higher divergence between sequences was observed in the cox1 fragment, with a maximum variation of 3.9%. The examination of eggs found in guanaco feces from southeastern Argentina and their specific molecular identification represent the first record for this host in Argentine Patagonia and contribute to improving the diagnosis of gastrointestinal nematodes in SACs, mainly in wild camelids.
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http://dx.doi.org/10.1016/j.parint.2018.10.007DOI Listing
February 2019

Checklist of helminths found in Patagonian wild mammals.

Authors:
Martin H Fugassa

Zootaxa 2015 Sep 3;4012(2):271-328. Epub 2015 Sep 3.

Laboratorio de Paleoparasitología, Universidad Nacional de Mar del Plata - CONICET. Calle Funes 3350, Mar del Plata (7600), Argentina.; Email:

Using available reports, a checklist of the recorded helminth parasites of wild mammals from Patagonia was generated. Records of parasites found in Patagonia were included, together with records from mammals in áreas outside of Patagonia but whose range extends into Patagonia. Information about the host, localities, and references were also included. A total of 1323 records (224 Cestoda, 167 Trematoda, 894 Nematoda, 34 Acanthocephala, and 4 Pentastomida) belonging to 452 helminth species (77 Cestoda, 76 Trematoda, 277 Nematoda, 21 Acanthocephala, and 1 Pentastomida) found in 57 native mammals (22 Rodentia, 4 Didelphimorphia 1 Microbiotheria, 7 Chiroptera, 5 Cingulata, and 13 Carnivora) were listed. However, only 10.6 % of the reports were conducted on samples from Patagonia and corresponded to 25% of mammals in the region. In addition, many studies were made on a few species and, for example, 52% corresponded to studies made on Lama guanicoe. This suggests the need to increase efforts to know the parasitic fauna in a peculiar region as is the Patagonia. This is the first compilation of the helminth parasites of mammals in Argentine Patagonia and is important for parasitological and paleoparasitological studies.
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http://dx.doi.org/10.11646/zootaxa.4012.2.3DOI Listing
September 2015

First palaeoparasitological record of a dioctophymatid egg in an archaeological sample from Patagonia.

Acta Trop 2013 Oct 14;128(1):175-7. Epub 2013 Jun 14.

CONICET-Lab. de Paleoparasitología y Arqueología Contextual, UNMdP, Argentina. Electronic address:

The collection of parasitological information from ancient material requires an exhaustive study of samples. In 2005, cestode and nematode eggs were found in a coprolite sample tentatively assigned to a canid. The sample was obtained from the layer of the archaeological site located in Cerro Casa de Piedra, Santa Cruz Province, Argentina, and dated from 6540±110 years before present. The aim of the present work was to reexamine this fixed sample in order to confirm the presence of these parasites. The palaeoparasitological results support our previous findings. Interestingly, another parasite was also confirmed: a dioctophymatid nematode. Dioctophyma renale has been reported in several modern carnivores in the Southern Hemisphere but in ancient materials, it has only been reported in human coprolites from Switzerland. This report constitutes the first evidence of the presence of a dioctophymatid nematode parasite dioctophymatid nematode in American pre-Columbian times. The results obtained in this work show the importance of revising earlier palaeoparasitological results.
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http://dx.doi.org/10.1016/j.actatropica.2013.06.001DOI Listing
October 2013

Parasitism of prehistoric humans and companion animals from Antelope Cave, Mojave County, northwest Arizona.

J Parasitol 2011 Oct 23;97(5):862-7. Epub 2011 Mar 23.

Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata 7600, Buenos Aires, Argentina.

Previously, we reported a tick recovered from Antelope Cave in extreme northwest Arizona. Further analyses of coprolites from Antelope Cave revealed additional parasitological data from coprolites of both human and canid origin. A second tick was found. This site is the only archaeological locality where ticks have been recovered. We also discovered an acanthocephalan in association with Enterobius vermicularis eggs in the same coprolite. This association shows that the coprolite was deposited by a human. This discovery expands our knowledge of the range of prehistoric acanthocephalan infection. In addition, findings from canid coprolites of Trichuris vulpis are reported. This is the first published discovery of T. vulpis from a North American archaeological context. The close association of dogs with humans at Ancestral Puebloan (Anasazi) sites raises the potential that zoonotic parasites were transferred to the human population. The archaeological occupation is associated with the Ancestral Pueblo culture 1,100 yr ago.
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http://dx.doi.org/10.1645/GE-2459.1DOI Listing
October 2011

Trichuris or Capillaria?

Authors:
Martin H Fugassa

Parasitol Int 2010 Mar 4;59(1):104. Epub 2010 Jan 4.

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http://dx.doi.org/10.1016/j.parint.2009.12.008DOI Listing
March 2010

Paleoparasitological analysis of rodent coprolites in holocenic samples from Patagonia, Argentina.

J Parasitol 2009 Jun;95(3):646-51

CONICET, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Funes 3250, (7600) Mar del Plata, Argentina.

The aim of the present study was to examine the parasite fauna present in rodent coprolites collected from Cerro Casa de Piedra (CCP7), located in Perito Moreno National Park (P.N.PM., 47 degrees 57'S and 72 degrees 05'W), Santa Cruz Province, Argentina. Eight coprolites obtained from the layer XIII of CCP7, with an antiquity considered as 7,920 +/- 130 yr B.P, were examined for parasites. Each coprolite was whole processed, rehydrated, homogenized, spontaneously sedimented, and examined using light microscopy. Eggs of parasites were measured and photographed. All the samples were parasitized by nematodes, with 267 eggs of Trichuris sp., 24 eggs of an aspidoderid, and 3 capillariid eggs. The rodent host was tentatively identified as a species of Ctenomys, the hypogeic rodents endemic to South America. The finding of Paraspidodera in Patagonian samples represents new evidence that strengthens the co-phylogenies between nematodes of this genus and Ctenomys and reinforces the value of parasites as tags in paleoparasitology.
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http://dx.doi.org/10.1645/GE-1809.1DOI Listing
June 2009

Capillaria spp. eggs in Patagonian archaeological sites: statistical analysis of morphometric data.

Mem Inst Oswaldo Cruz 2008 Feb;103(1):104-5

Laboratorio de Zoonosis Parasitarias, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Buenos Aires, Argentina.

Discriminant analysis was used to identify eggs of Capillaria spp. at specific level found in organic remains from an archaeological site in Patagonia, Argentina, dated of 6,540 +/- 110 years before present. In order to distinguish eggshell morphology 149 eggs were measured and grouped into four arbitrary subsets. The analysis used on egg width and length discriminated them into different morphotypes (Wilks' lambda = 0.381, p < 0.05). The correlation analysis suggests that width was the most important variable to discriminate among the Capillaria spp. egg morphotypes (Pearson coefficient = 0.950, p < 0.05). The study of eggshell patterns, the relative frequency in the sample, and the morphometric data allowed us to correlate the four morphotypes with Capillaria species.
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http://dx.doi.org/10.1590/s0074-02762008000100016DOI Listing
February 2008

Quantitative paleoparasitology applied to archaeological sediments.

Mem Inst Oswaldo Cruz 2006 Dec;101 Suppl 2:29-33

Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Buenos Aires, Argentina.

Three techniques to extract parasite remains from archaeological sediments were tested. The aim was to improve the sensibility of recommended paleoparasitological techniques applied in archaeological remains. Sediment collected from the pelvic girdle of a human body found in Cabo Vírgenes, Santa Cruz, Argentina, associated to a Spanish settlement founded in 1584 known as Nombre de Jesús, was used to search for parasites. Sediment close to the skull was used as control. The techniques recommended by Jones, Reinhard, and Dittmar and Teejen were used and compared with the modified technique presented here, developed to improve the sensibility to detect parasite remains. Positive results were obtained only with the modified technique, resulting in the finding of Trichuris trichiura eggs in the sediment.
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http://dx.doi.org/10.1590/s0074-02762006001000006DOI Listing
December 2006