Mark Howarth

Mark Howarth

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Mark Howarth

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Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions.

Biomaterials 2018 Oct 17;180:253-264. Epub 2018 Jul 17.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK. Electronic address:

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http://dx.doi.org/10.1016/j.biomaterials.2018.07.020DOI Listing
October 2018

Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination.

ACS Nano 2018 Sep 26;12(9):8855-8866. Epub 2018 Jul 26.

Department of Biochemistry , University of Oxford , South Parks Road , Oxford OX1 3QU , United Kingdom.

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http://dx.doi.org/10.1021/acsnano.8b02805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158681PMC
September 2018

Nanoteamwork: covalent protein assembly beyond duets towards protein ensembles and orchestras.

Curr Opin Biotechnol 2018 06 21;51:16-23. Epub 2017 Nov 21.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. Electronic address:

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http://dx.doi.org/10.1016/j.copbio.2017.10.006DOI Listing
June 2018

Editorial overview: Nanobiotechnology: Baby steps and giant strides towards molecular mastery.

Curr Opin Biotechnol 2018 06;51:iv-vi

Integrative Synthetic Biology Centre and School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK; CNRS-UMR8030, Laboratoire iSSB and Université Paris-Saclay and Université d'Évry and CEA, DRF, IG, Genoscope, Évry 91000, France; Institute for Integrative Systems Biology (I2SysBio), University of Valencia-CSIC, 46980 Paterna, Spain. Electronic address:

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http://dx.doi.org/10.1016/j.copbio.2018.05.001DOI Listing
June 2018

New Routes and Opportunities for Modular Construction of Particulate Vaccines: Stick, Click, and Glue.

Front Immunol 2018 26;9:1432. Epub 2018 Jun 26.

Department of Biochemistry, University of Oxford, Oxford, United Kingdom.

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http://dx.doi.org/10.3389/fimmu.2018.01432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028521PMC
June 2018

SnoopLigase Catalyzes Peptide-Peptide Locking and Enables Solid-Phase Conjugate Isolation.

J Am Chem Soc 2018 02 14;140(8):3008-3018. Epub 2018 Feb 14.

Department of Biochemistry, University of Oxford , South Parks Road, Oxford, OX1 3QU, U.K.

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http://dx.doi.org/10.1021/jacs.7b13237DOI Listing
February 2018

Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics.

Angew Chem Int Ed Engl 2017 Dec 5;56(52):16521-16525. Epub 2017 Dec 5.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.

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http://dx.doi.org/10.1002/anie.201707623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5814910PMC
December 2017

Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction.

Cell Chem Biol 2017 Aug 27;24(8):1040-1047.e4. Epub 2017 Jul 27.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. Electronic address:

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http://dx.doi.org/10.1016/j.chembiol.2017.06.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563079PMC
August 2017

Extracellular Self-Assembly of Functional and Tunable Protein Conjugates from Bacillus subtilis.

ACS Synth Biol 2017 06 7;6(6):957-967. Epub 2017 Mar 7.

Centre for Synthetic Biology and Innovation, Imperial College London , London SW7 2AZ, U.K.

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http://pubs.acs.org/doi/10.1021/acssynbio.6b00292
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http://dx.doi.org/10.1021/acssynbio.6b00292DOI Listing
June 2017

Dual Plug-and-Display Synthetic Assembly Using Orthogonal Reactive Proteins for Twin Antigen Immunization.

Bioconjug Chem 2017 05 5;28(5):1544-1551. Epub 2017 May 5.

Department of Biochemistry, University of Oxford , South Parks Road, Oxford OX1 3QU, United Kingdom.

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http://dx.doi.org/10.1021/acs.bioconjchem.7b00174DOI Listing
May 2017

Smart superglue in streptococci? The proof is in the pulling.

Authors:
Mark Howarth

J Biol Chem 2017 05;292(21):8998-8999

From the Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom

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http://dx.doi.org/10.1074/jbc.H117.777466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448131PMC
May 2017

Controlling Multivalent Binding through Surface Chemistry: Model Study on Streptavidin.

J Am Chem Soc 2017 03 9;139(11):4157-4167. Epub 2017 Mar 9.

Biosurfaces Lab, CIC biomaGUNE , Paseo Miramon 182, 20014 Donostia - San Sebastian, Spain.

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http://dx.doi.org/10.1021/jacs.7b00540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364436PMC
March 2017

Programmable polyproteams built using twin peptide superglues.

Proc Natl Acad Sci U S A 2016 Feb 19;113(5):1202-7. Epub 2016 Jan 19.

Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, United Kingdom;

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http://dx.doi.org/10.1073/pnas.1519214113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747704PMC
February 2016

SpyRing interrogation: analyzing how enzyme resilience can be achieved with phytase and distinct cyclization chemistries.

Sci Rep 2016 Feb 10;6:21151. Epub 2016 Feb 10.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.

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http://dx.doi.org/10.1038/srep21151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748275PMC
February 2016

Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher.

Curr Opin Chem Biol 2015 Dec 30;29:94-9. Epub 2015 Oct 30.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. Electronic address:

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http://dx.doi.org/10.1016/j.cbpa.2015.10.002DOI Listing
December 2015

Site-specific biotinylation of purified proteins using BirA.

Methods Mol Biol 2015 ;1266:171-84

Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.

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http://link.springer.com/10.1007/978-1-4939-2272-7_12
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http://dx.doi.org/10.1007/978-1-4939-2272-7_12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304673PMC
September 2015

Say it with proteins: an alphabet of crystal structures.

Authors:
Mark Howarth

Nat Struct Mol Biol 2015 May;22(5):349

Department of Biochemistry, Oxford University, Oxford, UK.

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http://dx.doi.org/10.1038/nsmb.3011DOI Listing
May 2015

Love-Hate ligands for high resolution analysis of strain in ultra-stable protein/small molecule interaction.

Bioorg Med Chem 2014 Oct 24;22(19):5476-86. Epub 2014 Jul 24.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S09680896140054
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http://dx.doi.org/10.1016/j.bmc.2014.07.029DOI Listing
October 2014

Superglue from bacteria: unbreakable bridges for protein nanotechnology.

Trends Biotechnol 2014 Oct 26;32(10):506-12. Epub 2014 Aug 26.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. Electronic address:

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http://dx.doi.org/10.1016/j.tibtech.2014.08.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281928PMC
October 2014

SpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly.

J Am Chem Soc 2014 Sep 21;136(35):12355-63. Epub 2014 Aug 21.

Department of Biochemistry, University of Oxford , South Parks Road, Oxford, OX1 3QU, U.K.

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http://pubs.acs.org/doi/10.1021/ja505584f
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http://dx.doi.org/10.1021/ja505584fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183622PMC
September 2014

SpyTag/SpyCatcher cyclization confers resilience to boiling on a mesophilic enzyme.

Angew Chem Int Ed Engl 2014 Jun 9;53(24):6101-4. Epub 2014 May 9.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU (UK).

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http://dx.doi.org/10.1002/anie.201402519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286826PMC
June 2014

SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture.

Proc Natl Acad Sci U S A 2014 Apr 17;111(13):E1176-81. Epub 2014 Mar 17.

Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, United Kingdom.

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http://www.pnas.org/lookup/doi/10.1073/pnas.1315776111
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http://dx.doi.org/10.1073/pnas.1315776111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977242PMC
April 2014

Plug-and-play pairing via defined divalent streptavidins.

J Mol Biol 2014 Jan 19;426(1):199-214. Epub 2013 Sep 19.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. Electronic address:

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http://dx.doi.org/10.1016/j.jmb.2013.09.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4047826PMC
January 2014

Structural analysis and optimization of the covalent association between SpyCatcher and a peptide Tag.

J Mol Biol 2014 Jan 23;426(2):309-17. Epub 2013 Oct 23.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. Electronic address:

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http://dx.doi.org/10.1016/j.jmb.2013.10.021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3959856PMC
January 2014

Association of water softness and heavy alcohol consumption with higher hospital admission rates for alcoholic liver disease.

Alcohol Alcohol 2012 Nov-Dec;47(6):688-96. Epub 2012 Sep 7.

The Institute of Hepatology, Foundation for Liver Research, 69-75 Chenies Mews, London, WC1E 6HX, UK.

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http://dx.doi.org/10.1093/alcalc/ags088DOI Listing
July 2013

Cholesterol loading and ultrastable protein interactions determine the level of tumor marker required for optimal isolation of cancer cells.

Cancer Res 2013 Apr 1;73(7):2310-21. Epub 2013 Feb 1.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford, United Kingdom.

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http://dx.doi.org/10.1158/0008-5472.CAN-12-2956DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3618857PMC
April 2013

Hydroxy-terminated conjugated polymer nanoparticles have near-unity bright fraction and reveal cholesterol-dependence of IGF1R nanodomains.

ACS Nano 2013 Feb 30;7(2):1137-44. Epub 2013 Jan 30.

Department of Biochemistry, Oxford University, South Parks Road, OX1 3QU, United Kingdom.

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http://dx.doi.org/10.1021/nn3042122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584654PMC
February 2013

Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.

ACS Nano 2012 Dec 5;6(12):11080-7. Epub 2012 Dec 5.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 18-496, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/nn304793zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528850PMC
December 2012

Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin.

Proc Natl Acad Sci U S A 2012 Mar 24;109(12):E690-7. Epub 2012 Feb 24.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.

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http://dx.doi.org/10.1073/pnas.1115485109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3311370PMC
March 2012

A peptide filtering relation quantifies MHC class I peptide optimization.

PLoS Comput Biol 2011 Oct 13;7(10):e1002144. Epub 2011 Oct 13.

Biological Computation Group, Microsoft Research, Cambridge, United Kingdom.

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http://dx.doi.org/10.1371/journal.pcbi.1002144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195949PMC
October 2011

Mechanisms for size-dependent protein segregation at immune synapses assessed with molecular rulers.

Biophys J 2011 Jun;100(12):2865-74

Sir Alexander Fleming Building, Division of Cell and Molecular Biology, Imperial College London, London, UK.

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http://dx.doi.org/10.1016/j.bpj.2011.05.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3123984PMC
June 2011

Spontaneous intermolecular amide bond formation between side chains for irreversible peptide targeting.

J Am Chem Soc 2010 Apr;132(13):4526-7

Department of Biochemistry, Oxford University, South Parks Road, Oxford, OX1 3QU, UK.

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http://pubs.acs.org/doi/abs/10.1021/ja910795a
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http://dx.doi.org/10.1021/ja910795aDOI Listing
April 2010

Electrophilic affibodies forming covalent bonds to protein targets.

J Biol Chem 2009 Nov 15;284(47):32906-13. Epub 2009 Sep 15.

Department of Biochemistry, Oxford University, South Parks Road, Oxford OX1 3QU, United Kingdom.

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http://dx.doi.org/10.1074/jbc.M109.034322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2781706PMC
November 2009

Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin.

Nat Protoc 2008 ;3(3):534-45

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1038/nprot.2008.20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2671200PMC
November 2008

Monovalent, reduced-size quantum dots for imaging receptors on living cells.

Nat Methods 2008 May 20;5(5):397-9. Epub 2008 Apr 20.

Department of Chemistry, 77 Massachusetts Avenue, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1038/nmeth.1206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2637151PMC
May 2008

Tapasin shapes immunodominance hierarchies according to the kinetic stability of peptide-MHC class I complexes.

Eur J Immunol 2008 Feb;38(2):364-9

Cancer Sciences Division, University of Southampton School of Medicine, SGH, Southampton, UK.

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http://doi.wiley.com/10.1002/eji.200737832
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http://dx.doi.org/10.1002/eji.200737832DOI Listing
February 2008

Compact biocompatible quantum dots functionalized for cellular imaging.

J Am Chem Soc 2008 Jan 5;130(4):1274-84. Epub 2008 Jan 5.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.

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http://dx.doi.org/10.1021/ja076069pDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665712PMC
January 2008

A monovalent streptavidin with a single femtomolar biotin binding site.

Nat Methods 2006 Apr;3(4):267-73

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1038/nmeth861DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2576293PMC
April 2006

Giving cells a new sugar-coating.

Nat Chem Biol 2006 Mar;2(3):127-8

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http://dx.doi.org/10.1038/nchembio0306-127DOI Listing
March 2006

DNA transfection screening from single beads.

J Comb Chem 2004 Sep-Oct;6(5):753-60

Department of Chemistry, University of Southampton, Southampton SO17 1BJ, U.K.

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http://dx.doi.org/10.1021/cc049945tDOI Listing
January 2006

Targeting quantum dots to surface proteins in living cells with biotin ligase.

Proc Natl Acad Sci U S A 2005 May 16;102(21):7583-8. Epub 2005 May 16.

Department of Chemistry, The Picower Center for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1073/pnas.0503125102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1129026PMC
May 2005

Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase.

Nat Methods 2005 Feb 21;2(2):99-104. Epub 2005 Jan 21.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Room 18-496, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1038/nmeth735DOI Listing
February 2005

Tapasin enhances MHC class I peptide presentation according to peptide half-life.

Proc Natl Acad Sci U S A 2004 Aug 30;101(32):11737-42. Epub 2004 Jul 30.

Medical Research Council Human Immunology Unit, Oxford University, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom.

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http://dx.doi.org/10.1073/pnas.0306294101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC511045PMC
August 2004

The processing of antigens delivered as DNA vaccines.

Immunol Rev 2004 Jun;199:27-39

Cancer Sciences Division, University of Southampton School of Medicine, Southampton General Hospital, Southampton, UK.

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http://dx.doi.org/10.1111/j.0105-2896.2004.00141.xDOI Listing
June 2004

Solid-phase synthesis of 89 polyamine-based cationic lipids for DNA delivery to mammalian cells.

Chemistry 2004 Jan;10(2):463-73

Department of Chemistry, University of Southampton, Southampton SO17 1BJ, UK.

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http://dx.doi.org/10.1002/chem.200305232DOI Listing
January 2004

Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin.

Immunity 2002 Jan;16(1):99-109

University of Oxford, Nuffield Department of Clinical Medicine, Institute for Molecular Medicine, John Radcliffe Hospital, OX3 9DS, Oxford, United Kingdom.

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January 2002