Publications

PDZ domain-dependent regulation of NHE3 protein by both internal Class II and C-terminal Class I PDZ-binding motifs.
J Biol Chem 2017 May 10;292(20):8279-8290. Epub 2017 Mar 10.
From the Departments of Physiology and Medicine, Gastroenterology Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Phenylquinoxalinone CFTR activator as potential prosecretory therapy for constipation.
Transl Res 2017 Apr 15;182:14-26.e4. Epub 2016 Oct 15.
Departments of Medicine and Physiology, University of California San Francisco, San Francisco, Calif. Electronic address:




Human Enteroids/Colonoids and Intestinal Organoids Functionally Recapitulate Normal Intestinal Physiology and Pathophysiology.
J Biol Chem 2016 Feb 16;291(8):3759-66. Epub 2015 Dec 16.
From the Division of Gastroenterology and Hepatology, Department of Medicine, and Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205,

Enterohemorrhagic Escherichia coli reduce mucus and intermicrovillar bridges in human stem cell-derived colonoids.
Cell Mol Gastroenterol Hepatol 2016 Jan;2(1):48-62.e3
Department of Medicine, Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, MD.


Human Intestinal Enteroids: a New Model To Study Human Rotavirus Infection, Host Restriction, and Pathophysiology.
J Virol 2015 Oct 7;90(1):43-56. Epub 2015 Oct 7.
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA Department of Medicine, Baylor College of Medicine, Houston, Texas, USA

The NHERF2 sequence adjacent and upstream of the ERM-binding domain affects NHERF2-ezrin binding and dexamethasone stimulated NHE3 activity.
Biochem J 2015 Aug 16;470(1):77-90. Epub 2015 Jun 16.
Departments of Medicine, Division of Gastroenterology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, U.S.A. Department of Physiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, U.S.A.


Human enteroids as an ex-vivo model of host-pathogen interactions in the gastrointestinal tract.
Exp Biol Med (Maywood) 2014 Sep 9;239(9):1124-34. Epub 2014 Apr 9.
Department of Medicine, Division of Gastroenterology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

Myosin VI mediates the movement of NHE3 down the microvillus in intestinal epithelial cells.
J Cell Sci 2014 Aug 13;127(Pt 16):3535-45. Epub 2014 Jun 13.
Department of Medicine, Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA Departments of Medicine and Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

Lysophosphatidic acid stimulation of NHE3 exocytosis in polarized epithelial cells occurs with release from NHERF2 via ERK-PLC-PKCδ signaling.
Am J Physiol Cell Physiol 2014 Jul 23;307(1):C55-65. Epub 2014 Apr 23.
Departments of Physiology and Medicine, Gastrointestinal Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland; and

NHERF2/NHERF3 protein heterodimerization and macrocomplex formation are required for the inhibition of NHE3 activity by carbachol.
J Biol Chem 2014 Jul 27;289(29):20039-53. Epub 2014 May 27.
From the Department of Medicine, Division of Gastroenterology and Department of Physiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

AKT and GSK-3 are necessary for direct ezrin binding to NHE3 as part of a C-terminal stimulatory complex: role of a novel Ser-rich NHE3 C-terminal motif in NHE3 activity and trafficking.
J Biol Chem 2014 Feb 7;289(9):5449-61. Epub 2014 Jan 7.
From the Departments of Physiology and Medicine, Gastroenterology Division, Johns, Hopkins University School of Medicine, Baltimore, Maryland 21205 and.

Translating molecular physiology of intestinal transport into pharmacologic treatment of diarrhea: stimulation of Na+ absorption.
Clin Gastroenterol Hepatol 2014 Jan 31;12(1):27-31. Epub 2013 Oct 31.
Departments of Physiology and Medicine, Gastroenterology Division, Johns Hopkins University School of Medicine, Baltimore, Maryland. Electronic address:

NHERF2 protein mobility rate is determined by a unique C-terminal domain that is also necessary for its regulation of NHE3 protein in OK cells.
J Biol Chem 2013 Jun 23;288(23):16960-74. Epub 2013 Apr 23.
Department of Medicine, Division of Gastroenterology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Calmodulin kinase II constitutively binds, phosphorylates, and inhibits brush border Na+/H+ exchanger 3 (NHE3) by a NHERF2 protein-dependent process.
J Biol Chem 2012 Apr 27;287(16):13442-56. Epub 2012 Feb 27.
Division of Gastroenterology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

NHERF1 and NHERF2 are necessary for multiple but usually separate aspects of basal and acute regulation of NHE3 activity.
Am J Physiol Cell Physiol 2011 Apr 29;300(4):C771-82. Epub 2010 Dec 29.
Gastroenterology and Hepatology Division, Department of Medicine, Johns Hopkins Univ. School of Medicine, Baltimore, MD 21205-2195, USA.

D-glucose acts via sodium/glucose cotransporter 1 to increase NHE3 in mouse jejunal brush border by a Na+/H+ exchange regulatory factor 2-dependent process.
Gastroenterology 2011 Feb 23;140(2):560-71. Epub 2010 Oct 23.
Department of Medicine, Division of Gastroenterology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.


NHE3 activity is dependent on direct phosphoinositide binding at the N terminus of its intracellular cytosolic region.
J Biol Chem 2010 Nov 24;285(45):34566-78. Epub 2010 Aug 24.
Department of Physiology and Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

NHE3 mobility in brush borders increases upon NHERF2-dependent stimulation by lyophosphatidic acid.
J Cell Sci 2010 Jul 22;123(Pt 14):2434-43. Epub 2010 Jun 22.
Departments of Physiology and Medicine, Gastroenterology Division, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 212052, USA.

Mutations in TTC37 cause trichohepatoenteric syndrome (phenotypic diarrhea of infancy).
Gastroenterology 2010 Jun 20;138(7):2388-98, 2398.e1-2. Epub 2010 Feb 20.
Department of Medical and Molecular Genetics, School of Clinical and Experimental Medicine, University of Birmingham College of Medical and Dental School, Institute of Biomedical Research, Edgbaston, Birmingham, UK.


Lysophosphatidic acid stimulates the intestinal brush border Na(+)/H(+) exchanger 3 and fluid absorption via LPA(5) and NHERF2.
Gastroenterology 2010 Feb 1;138(2):649-58. Epub 2009 Oct 1.
Division of Digestive Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA.

Phospholipase C-gamma binds directly to the Na+/H+ exchanger 3 and is required for calcium regulation of exchange activity.
J Biol Chem 2009 Jul 27;284(29):19437-44. Epub 2009 May 27.
Division of Gastroenterology and Hepatology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Differential roles of NHERF1, NHERF2, and PDZK1 in regulating CFTR-mediated intestinal anion secretion in mice.
J Clin Invest 2009 Mar 16;119(3):540-50. Epub 2009 Feb 16.
Department of Gastroenterology, Hepatology, and Endocrinology, Carl-Neuberg-Strasse 1, Hannover Medical School, Hannover, Germany.


Macropinocytosis in Shiga toxin 1 uptake by human intestinal epithelial cells and transcellular transcytosis.
Am J Physiol Gastrointest Liver Physiol 2009 Jan 30;296(1):G78-92. Epub 2008 Oct 30.
Dept. of Medicine, Division of Gastroenterology, 918 Ross Research Bldg., 720 Rutland Ave., Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.

Casein kinase 2 binds to the C terminus of Na+/H+ exchanger 3 (NHE3) and stimulates NHE3 basal activity by phosphorylating a separate site in NHE3.
Mol Biol Cell 2008 Sep 9;19(9):3859-70. Epub 2008 Jul 9.
Division of Gastroenterology and Hepatology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.


Urine electrolyte, mineral, and protein excretion in NHERF-2 and NHERF-1 null mice.
Am J Physiol Renal Physiol 2008 Apr 6;294(4):F1001-7. Epub 2008 Feb 6.
Univ. of Maryland School of Medicine, 22 S. Greene St., N3W143, Baltimore, MD 21201, USA.


cGMP inhibition of Na+/H+ antiporter 3 (NHE3) requires PDZ domain adapter NHERF2, a broad specificity protein kinase G-anchoring protein.
J Biol Chem 2005 Apr 18;280(17):16642-50. Epub 2005 Feb 18.
Department of Physiology, GI Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.


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