Publications by authors named "Marisa Espinazo"

3 Publications

  • Page 1 of 1

Phenotypic Effects and Inhibition of Botrydial Biosynthesis Induced by Different Plant-Based Elicitors in Botrytis cinerea.

Curr Microbiol 2018 Apr 17;75(4):431-440. Epub 2017 Nov 17.

Microbiology Laboratory, Andalusian Center for Grape and Grapevine Research, Faculty of Marine and Environmental Sciences, University of Cadiz, Polígono Rio San Pedro s/n, 11510, Puerto Real, Cádiz, Spain.

Botrytis cinerea is considered a model organism for the study of plant-pathogen interaction showing great genetic diversity and a high degree of morphological variability depending on environmental conditions. The use of new compounds and plant-based elicitors may trigger the expression of different B. cinerea genes, providing new sources of virulence factors. This work is focused on elucidating the phenotypic effect in B. cinerea of different carbon sources such as glucose, cellulose and tomato cell walls (TCW). Production of botrydial and dihydrobotrydial toxins was evaluated using thin-layer chromatography (TLC), proton nuclear magnetic resonance spectroscopy (H-NMR) and mass spectrometry (UPLC-HRESIMS). Expression of the toxin biosynthesis gene BcBOT2 was followed using RT-qPCR. Results show an inhibition of the toxin biosynthesis pathway when TCW are present as a sole carbon source, suggesting that the toxin is only produced when rich molecules, like glucose, are available for fungal metabolism. That suggests a connection between gene expression of virulence factors and environmental conditions, where the silent genes can be induced by different culture conditions.
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http://dx.doi.org/10.1007/s00284-017-1399-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5842495PMC
April 2018

Author response.

Ann Allergy Asthma Immunol 2014 Feb;112(2):185

Clínica Dr. Lobatón, Cádiz, Spain.

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http://dx.doi.org/10.1016/j.anai.2013.12.002DOI Listing
February 2014

Measurement of IgE to pollen allergen components is helpful in selecting patients for immunotherapy.

Ann Allergy Asthma Immunol 2013 Oct 2;111(4):295-7. Epub 2013 Aug 2.

Clínica Dr Lobatón, Cádiz, Spain. Electronic address:

Background: Pollen allergy still represents an important cause of allergic morbidity worldwide. Accurate diagnostic methods are important to determine the disease-causing allergen.

Objective: To describe the sensitization patterns of patients with spring pollinosis and to make a real-life evaluation of the usefulness of a predetermined IgE molecular profile compared with other sensitization methods for choosing the composition of immunotherapy.

Methods: One hundred seventy-five patients with a diagnosis of spring pollinosis completed a skin prick test study with Olea europaea, Phleum pratense, palm profilin, and peach peel and an in vitro study of the application of a specific recombinant IgE protocol (nOle e1, rPhl p1-5b, rPhl p12, rPhl p7, and rPru p3). Immunotherapy using the 2 methods was compared.

Results: A high sensitization to nOle e1 and rPhl p1-5b was found. Profilin, polcalcin, and lipid transfer proteins seemed irrelevant for the differential diagnosis of olea and grass pollen sensitization in the most southern area of the Iberian Peninsula. Application of the component resolved the diagnosis, and the choice of immunotherapy was changed in more than 50% of patients.

Conclusion: These results support the necessity of the habitual use of this kind of protocol in routine allergologic practice.
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http://dx.doi.org/10.1016/j.anai.2013.07.005DOI Listing
October 2013