Publications by authors named "Marina Y Zemskova"

3 Publications

  • Page 1 of 1

Integrin Alpha V in Urine: A Novel Noninvasive Marker for Prostate Cancer Detection.

Front Oncol 2020 10;10:610647. Epub 2021 Mar 10.

Department of the Research, Prostagnost LLC, Moscow, Russia.

Prostate cancer (PCa) diagnosis based on patient urine analysis provides non-invasive and promising method as compared to biopsy and a prostate-specific antigen (PSA) test. This study was conceived to investigate whether Integrin alpha V (ITGAV) protein is present in urine and assess the urinary ITGAV diagnostic potential for PCa.

Materials And Methods: Urinary ITGAV expression was determined by Western blot analysis and quantified by ELISA in urine from men with PCa (n = 47), benign prostate hyperplasia (n = 42) and age-matched controls (n = 22).

Results: The level of ITGAV protein was significantly lower in PCa urine samples as compared to those in the control group (p < 0.00001). The decrease of ITGAV in urine was highly predictive of PCa with 91.5% sensitivity, 91.4% specificity, 0.93 area under the ROC curve, and its specificity was better than that of serum PSA.

Conclusion: Urinary ITGAV provides a novel noninvasive biomarker with high specificity.
View Article and Find Full Text PDF

Download full-text PDF

Source Listing
March 2021

Austalides V and W, new meroterpenoids from the fungus Aspergillus ustus and their antitumor activities.

Bioorg Med Chem Lett 2019 11 4;29(22):126708. Epub 2019 Oct 4.

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms RAS, FSBIS FRC Pushchino Scientific Centre of Biological Research, Russian Academy of Sciences, Pushchino 142290, Russian Federation.

Two new austalide meroterpenoids, named austalides V and W (1 and 2), were isolated from the fungus Aspergillus ustus VKM F-4692. Their structures were elucidated by extensive spectroscopic analysis and by comparison with related known compounds. The main structural feature of both compounds is a tetrahydrofuranyl ring (G), a structural fragment, first found in austalides. Austalides V (1) and W (2) were able to inhibit the propagation of prostate and bladder cancer cells; this biologic activity is possibly related to the inhibition of a number of key pathways regulating cell growth and migration.
View Article and Find Full Text PDF

Download full-text PDF

Source Listing
November 2019

Regulation of prostate stromal fibroblasts by the PIM1 protein kinase.

Cell Signal 2015 Jan 28;27(1):135-46. Epub 2014 Oct 28.

Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, United States; The Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, United States. Electronic address:

The PIM1 oncogene is over-expressed in human prostate cancer epithelial cells. Importantly, we observe that in human hyperplastic and cancerous prostate glands PIM1 is also markedly elevated in prostate fibroblasts, suggesting an important role for this kinase in epithelial/stromal crosstalk. The ability of PIM1 to regulate the biologic activity of stromal cells is demonstrated by the observation that expression of PIM1 kinase in human prostate fibroblasts increases the level and secretion of the extracellular matrix molecule, collagen 1A1 (COL1A1), the pro-inflammatory chemokine CCL5, and the platelet-derived growth factor receptors (PDGFR). PIM1 is found to regulate the transcription of CCL5. In co-cultivation assays where PIM1 over-expressing fibroblasts are grown with BPH1 prostate epithelial cells, PIM1 activity markedly enhances the ability of these fibroblasts to differentiate into myofibroblasts and express known markers of cancer-associated fibroblasts (CAFs). This differentiation can be reversed by the addition of small molecule PIM kinase inhibitors. Western blots demonstrate that PIM1 expression in prostate fibroblasts stimulates the phosphorylation of molecules that regulate 5'Cap driven protein translation, including 4EBP1 and eIF4B. Consistent with the hypothesis that the kinase controls translation of specific mRNAs in prostate fibroblasts, we demonstrate that PIM1 expression markedly increases the level of COL1A1 and PDGFRβ mRNA bound to polysomes. Together these results point on PIM1 as a novel factor in regulation of the phenotype and differentiation of fibroblasts in prostate cancer by controlling both the transcription and translation of specific mRNAs.
View Article and Find Full Text PDF

Download full-text PDF

Source Listing
January 2015