Publications by authors named "Mariko Kanai"

5 Publications

  • Page 1 of 1

Genomic and Genetic Approaches to Studying Antimalarial Drug Resistance and Plasmodium Biology.

Trends Parasitol 2021 06 11;37(6):476-492. Epub 2021 Mar 11.

Department of Microbiology & Immunology, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA; Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA. Electronic address:

Recent progress in genomics and molecular genetics has empowered novel approaches to study gene functions in disease-causing pathogens. In the human malaria parasite Plasmodium falciparum, the application of genome-based analyses, site-directed genome editing, and genetic systems that allow for temporal and quantitative regulation of gene and protein expression have been invaluable in defining the genetic basis of antimalarial resistance and elucidating candidate targets to accelerate drug discovery efforts. Using examples from recent studies, we review applications of some of these approaches in advancing our understanding of Plasmodium biology and illustrate their contributions and limitations in characterizing parasite genomic loci associated with antimalarial drug responses.
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http://dx.doi.org/10.1016/j.pt.2021.02.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162148PMC
June 2021

Ionic liquid/ZnO(0001[combining macron]) single crystal and epitaxial film interfaces studied through a combination of electrochemical measurements and a pulsed laser deposition process under vacuum.

Phys Chem Chem Phys 2019 Dec 12;21(45):25506-25512. Epub 2019 Nov 12.

Department of Applied Chemistry, School of Engineering, Tohoku University, 6-6-07 Aramaki Aza Aoba, Aoba-ku Sendai, 980-8579, Japan.

O-Polar ZnO(0001[combining macron]) single crystals and ZnO and Mg-doped ZnO (MgZnO) films which were subsequently deposited on the ZnO crystals by a pulsed laser deposition (PLD) method were electrochemically investigated through the interfaces with ionic liquid (IL) in a vacuum. The sample surfaces were confirmed to be atomically clean and flat by reflection high energy electron diffraction (RHEED) observation, prior to their electrochemical measurements. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were then performed, and the donor density, flat band potential of these ZnO samples, and the electric double layer capacitance at the IL/ZnO interfaces were successfully evaluated. The flat band potentials of ZnO and MgZnO films were found to shift to more negative potentials relative to those of the single crystal ZnO, with different values for thicker films, respectively. Some possible origins of the different flat band potentials between ZnO and MgZnO films, and their film thickness dependence of the flat band potential will be discussed in this paper.
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http://dx.doi.org/10.1039/c9cp04875hDOI Listing
December 2019

Dual IFN-γ/hypoxia priming enhances immunosuppression of mesenchymal stromal cells through regulatory proteins and metabolic mechanisms.

J Immunol Regen Med 2018 Mar 25;1:45-56. Epub 2018 Apr 25.

Department of Biomedical Engineering, Columbia University, New York, NY, USA.

The immunosuppressive capacity of human mesenchymal stromal cells (MSCs) renders them promising candidates for treating diverse immune disorders. However, after hundreds of clinical trials, there are still no MSC therapies approved in the United States. MSCs require specific cues to adopt their immunosuppressive phenotype, and yet most clinical trials use cells expanded in basic culture medium and growth conditions. We propose that priming MSCs prior to administration will improve their therapeutic efficacy. Interferon-gamma (IFN-γ) priming are cues common to situations of immune escape that have individually shown promise as MSC priming cues but have not been systematically compared. Using mixed lymphocyte reactions, we show that priming MSCs with either cue alone improves T-cell inhibition. However, combining the two cues results in additive effects and markedly enhances the immunosuppressive phenotype of MSCs. We demonstrate that IFN-γ induces expression of numerous immunosuppressive proteins (IDO, PD-L1, HLA-E, HLA-G), whereas hypoxia switches MSCs to glycolysis, causing rapid glucose consumption and production of T-cell inhibitory lactate levels. Dual IFN-γ/hypoxia primed MSCs display both attributes and have even higher induction of immunosuppressive proteins over IFN-γ priming alone (IDO and HLA-G), which may reflect another benefit of metabolic reconfiguration.
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http://dx.doi.org/10.1016/j.regen.2018.01.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197483PMC
March 2018

Cardiac recovery via extended cell-free delivery of extracellular vesicles secreted by cardiomyocytes derived from induced pluripotent stem cells.

Nat Biomed Eng 2018 May 23;2(5):293-303. Epub 2018 Apr 23.

Department of Medicine, Columbia University, New York, NY, USA.

The ability of extracellular vesicles (EVs) to regulate a broad range of cellular processes has recently been exploited for the treatment of diseases. For example, EVs secreted by stem cells injected into infarcted hearts can induce recovery through the delivery of stem-cell-specific miRNAs. However, the retention of the EVs and the therapeutic effects are short-lived. Here, we show that an engineered hydrogel patch capable of slowly releasing EVs secreted from cardiomyocytes derived from induced pluripotent stem (iPS) cells reduced arrhythmic burden, promoted ejection-fraction recovery, decreased cardiomyocyte apoptosis 24 hours after infarction, and reduced infarct size and cell hypertrophy 4 weeks post-infarction when implanted onto infarcted rat hearts. We also show that the EVs are enriched with cardiac-specific miRNAs known to modulate cardiomyocyte-specific processes. The extended delivery of EVs secreted from iPS-cell-derived cardiomyocytes into the heart may help understand heart recovery and treat heart injury.
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http://dx.doi.org/10.1038/s41551-018-0229-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159913PMC
May 2018

Baicalein 5,6,7-trimethyl ether, a flavonoid derivative, stimulates fatty acid beta-oxidation in skin fibroblasts of X-linked adrenoleukodystrophy.

FEBS Lett 2005 Jan;579(2):409-14

Department of Biological Chemistry, Faculty of Pharmaceutical Science, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.

The purpose of the present study is to identify bioactive compounds with potential for X-linked adrenoleukodystrophy (X-ALD) pharmacological therapy. Various plant natural products including flavonoids were tested for their ability to ameliorate the abnormality of very long chain fatty acid (VLCFA) metabolism in cultured skin-fibroblasts from X-ALD patients. Of the compounds tested, baicalein 5,6,7-trimethyl ether (baicalein-tri-Me) was found to significantly stimulate the VLCFA beta-oxidation activity. Furthermore, the incorporation of [1-(14)C]lignoceric acid into cholesteryl esters was markedly reduced towards the normal level and the VLCFA (C24:0 and C26:0) content was decreased. These results make baicalein-tri-Me a candidate for the therapeutic compound for X-ALD.
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http://dx.doi.org/10.1016/j.febslet.2004.11.102DOI Listing
January 2005
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