Publications by authors named "Mariana Georgina Corigliano"

2 Publications

  • Page 1 of 1

Close relationship between the state of the oxygen evolving complex and rice cold stress tolerance.

Plant Sci 2020 Jul 9;296:110488. Epub 2020 Apr 9.

Laboratorio de Fisiología de Estrés Abiótico en Plantas, Unidad de Biotecnología 1, INTECH-CONICET-UNSAM, Chascomús, Argentina. Electronic address:

The results of the present work suggested a relationship between the growth stability and functional/structural parameters associated to the primary photochemistry and oxygen evolving complex (OEC) in tolerant rice plants under suboptimal low temperatures (SLT) stress. This was concluded from the absence of changes in net photosynthetic rate and in fraction of reaction centers to reduce quinone A, and very small changes in P680 efficiency to trap and donate electrons to quinone A and in fraction of active OEC in tolerant plants under cold stress but not in sensitive plants. The SLT stress also induced OEC activity limitations in both genotypes, but in a greater extent in sensitive plants. However, an assay using an artificial electron donor to replace OEC indicated that the P680 capacity to accept electrons was not altered in both genotypes under SLT stress from the beginning of the stress treatment, suggesting that the OEC structure stability is related to rice SLT tolerance to sustain the photosynthesis. This hypothesis was also supported by the fact that tolerant plants but not sensitive plants did not alter the gene expression and protein content of PsbP under SLT stress, an OEC subunit with a role in stabilizing of OEC structure.
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http://dx.doi.org/10.1016/j.plantsci.2020.110488DOI Listing
July 2020

Characterization of the plasmidic or chromosomal cpe gene and metabolic activities in Clostridium perfringens isolates from food in San Luis--Argentina.

Cent Eur J Public Health 2011 Mar;19(1):46-53

General Microbiology, Area of Microbiology, Department of Biochemistry and Biological Sciences, Faculty of Chemistry, Biochemistry and Pharmacy, San Luis National University, Argentina.

Food poisoning and non-food poisoning illnesses due to C. perfringens (by enterotoxin production) have been associated to chromosomal or plasmidic location of the cpe gene, respectively. Clostridial pathogenicity has been correlated to protease and azoreductase production. The aim of this work was: i) to assess the sanitary-hygienic quality of dehydrated soups (100 samples) consumed in San Luis - Argentina; ii) to verify the presence of C. perfringens in these food products using the "Most Probable Number" method (MPN) and plate-counting methods; iii) to characterise enterotoxigenicity in strain isolates by RPLA; iv) to determine the chromosomal or plasmidic location of the cpe gene in enterotoxigenic strains previously isolated from food in our lab, using PCR; v) to correlate chromosomal cpe and spore heat-resistance; vi) to compare protease activity in cpe+ and cpe- strains; and vii) to compare azoreductase activity in cpe+ and cpe- strains. Twenty-six isolates had a count a 3-43 bacteria g(-1) count using MPN; 7.7% exceeded the Argentine Food Code (CAA) limit. All isolates showed protease activity: enterotoxigenic isolates had higher protease activity than non-enterotoxigenic isolates. All isolates showed azoreductase activity: enterotoxigenic isolates had higher activity and shorter reducing times. Enterotoxigenic isolates showed chromosomal location for the gene responsible for the enterotoxin.
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http://dx.doi.org/10.21101/cejph.a3597DOI Listing
March 2011
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