Publications by authors named "Maria Márcia Pereira Sartori"

9 Publications

  • Page 1 of 1

Glyphosate applied at a hormetic dose improves ripening without impairing sugarcane productivity and ratoon sprouting.

Sci Total Environ 2022 Feb 23;806(Pt 2):150503. Epub 2021 Sep 23.

Laboratory of Ecophysiology Applied to Agriculture (LECA), School of Agricultural Sciences, São Paulo State University (UNESP), Botucatu, SP, Brazil.

The management of sugarcane ripening is essential to ensuring the supply of high-quality raw material for the sugar-alcohol industry; chemical ripeners are frequently used to accelerate sucrose accumulation in the stalks during harvesting. The potential ripening effect of a low dose of glyphosate was evaluated in sugarcane, along with its impact on productivity and sprouting in the next crop cycle. A field experiment was conducted in 2015 and 2016 using a randomized block design with eight replicates in a split-plot scheme, with the following treatments: (1) control with only water application, (2) glyphosate at a low dose of 1.8 g a.e. ha (corresponding to 0.005 L ha of the commercial product (cp)), and (3) glyphosate at the commercially recommended dose for a ripener at 180 g a.e. ha (corresponding to 0.50 L ha of the cp) applied at 60, 45, 30, and 15 days before harvest (DBH). The harvest was performed on May 25, 2016 (0 DBH), and a total of five periods were evaluated. This study showed that the application of a hormetic dose of glyphosate to stimulate sugarcane ripening is promising, despite the limited duration of the effect. The application of the hormetic dose (1.8 g a.e. ha) at 30 DBH improved the technological quality of sugarcane in terms of Brix% juice, pol% cane, purity% juice, moisture% cane, reducing sugars, total reducing sugars, and total recoverable sugar. Additionally, it increased pol productivity, and did not affect ratoon sprouting in the subsequent cycle. Thus, this study provides a strategy for ripening management with a low environmental impact for sugarcane producers through a low (hormetic) dose of glyphosate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.150503DOI Listing
February 2022

Acquisition of the physiological quality of peanut (Arachis hypogaea L.) seeds during maturation under the influence of the maternal environment.

PLoS One 2021 3;16(5):e0250293. Epub 2021 May 3.

Crop Production Department, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.

The scarcity of information on the maturation physiology of the peanut seed (Arachis hypogaea L.; Virgínia group) makes harvesting high quality seeds a challenge for the seed industry. During two consecutive crop seasons, we studied the acquisition of physiological quality of peanut seeds during maturation in tropical conditions. We bring new insights about the period of late maturation of seeds and the influence of the maternal environment on physiological quality. We monitored water content, dry weight, ability of germination, desiccation tolerance, vigor and longevity. In addition, we monitored temperature and precipitation throughout plant growth. We demonstrate that the physiological quality of peanut seeds is acquired during development, with a maximum between 57 and 76 days after flowering in the late stage of maturation. This final period represents about 25% of the development, considered the best time to harvest peanut seeds with the highest quality. Our findings also support the idea that the adequate proportion of rainfall and thermal sum in the maternal environment are factors that favor the acquisition of peanut seed longevity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250293PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092650PMC
October 2021

Effects of cowpea mild mottle virus on soybean cultivars in Brazil.

PeerJ 2020 31;8:e9828. Epub 2020 Aug 31.

Department of Plant Protection, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP), Botucatu, São Paulo, Brazil.

Soybean stem necrosis is caused by cowpea mild mottle virus (CPMMV), transmitted by the whitefly . CPMMV has already been recorded in all major soybean-producing areas of Brazil. The impacts caused by CPMMV to the current Brazilian soybean production are unknown, thus the main objective of this study was to evaluate the effects of CPMMV infection on the main important soybean cultivars grown in the Southern and Midwestern regions of Brazil. Although asymptomatic in some of the tested cultivars, CPMMV infection significantly reduced the plant height, the number of pods per plant and the 1,000-grain weight. In addition, estimated yield losses ranged from 174 to 638 kg ha, depending on the cultivar. Evidence of seed transmission of CPMMV was observed in the BMX POTÊNCIA RR cultivar. These results suggest that CPMMV could have an important role in the reduction of soybean productivity in Brazil, but symptomless infections might be hiding the actual impact of this pathogen in commercial fields and infected seeds could be the primary inoculum source of the virus in the field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.9828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469931PMC
August 2020

Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases.

J Venom Anim Toxins Incl Trop Dis 2019 13;25:e148718. Epub 2019 May 13.

Center for the Study of Venoms and Venomous Animals (CEVAP), UNESP - São Paulo State University, Botucatu, SP, Brazil.

Background: Ruminant feed containing animal byproduct proteins (ABPs) is prohibited in many countries due to its risk of transmitting prion diseases (PD). In most cases the entire herd is sacrificed, which causes great harm to the producer countries by preventing their exportation of ruminant derived-products.

Methods: We used stable isotope ratio mass spectrometry (IRMS) of carbon (C/C) and nitrogen (N/N) to trace the animal protein in the blood of 15 buffaloes () divided into three experimental groups: 1 - received only vegetable protein (VP) during 117 days; 2 - received animal and vegetable protein (AVP); and 3 - received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets containing 13.7% bovine meat and bone meal (MBM) added to a vegetable diet (from days 21-117 in the AVP group and until day 47 in the AVPR group, when MBM was removed).

Results: On the 36th day, differences were detectable in the feeding profile (p <0.01) among the three experimental groups, which remained for a further 49 days (85th day). The AVPR group showed isotopic rate reversibility on the 110th day by presenting values similar to those in the control group (VP) (p> 0.05), indicating that it took 63 days to eliminate MBM in this group. Total atoms exchange (> 95%) of C and N was observed through incorporation of the diet into the AVP and AVPR groups.

Conclusions: IRMS is an accurate and sensitive technique for tracing the feeding profile of ruminants through blood analysis, thus enabling investigation of ABP use.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1590/1678-9199-JVATITD-1487-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521725PMC
May 2019

Quantification of soy protein using the isotope method (δ(13)C and δ(15)N) for commercial brands of beef hamburger.

Meat Sci 2016 Dec 22;122:97-100. Epub 2016 Jul 22.

Center of Stable Isotopes, Institute of Biosciences, Univ. Estadual Paulista - UNESP, Distrito de Rubião Júnior, s/n, Botucatu, SP Postal Code 18.618-970, Brazil.

Hamburgers (beef patties) may be adulterated through the overuse of protein extenders. Among vegetables, soy protein is the best substitute for animal protein. These ingredients help to reduce the cost of producing a final product, and they maximize profits for fraudulent industries. Moreover, the ingestion of soy or other non-meat proteins by allergic individuals may present a health risk. In addition, monitoring by supervisory bodies is hampered by a lack of appropriate analytical methodologies. Within this context, the aim of this study was to determine and quantify the levels of added soy protein by determination of (15)N and (13)C stable isotopes. A total of 100 beef hamburger samples from 10 commercial brands were analyzed. Only three samples of the G brand were within the standards set the Brazilian legislation. The remaining 97 samples from 10 commercial brands contained >4% soy protein; therefore, they are adulterated and not in compliance with the current legislation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.meatsci.2016.07.012DOI Listing
December 2016

Effect of Glutamine, Glutamic Acid and Nucleotides on the Turnover of Carbon (δ(13)C) in Organs of Weaned Piglets.

Asian-Australas J Anim Sci 2016 Aug 3;29(8):1152-8. Epub 2015 Sep 3.

Institute of Biosciences, São Paulo State University (UNESP), Environmental Stable Isotopes Center, Botucatu, São Paulo 18618-970, Brazil.

Morphological and physiological alterations occur in the digestive system of weanling piglets, compromising the performance in subsequent phases. This experiment aimed at verifying the influence of glutamine, glutamate and nucleotides on the carbon turnover in the pancreas and liver of piglets weaned at 21 days of age. Four diets were evaluated: glutamine, glutamic acid or nucleotides-free diet (CD); containing 1% glutamine (GD); containing 1% glutamic acid (GAD) and containing 1% nucleotides (ND). One hundred and twenty-three piglets were utilized with three pigs slaughtered at day zero (weaning day) and three at each one of the experimental days (1, 2, 4, 5, 7, 9, 13, 20, 27, and 49 post-weaning), in order to collect organ samples, which were analyzed for the δ(13)C isotopic composition and compared by means of time. No differences were found (p>0.05) among treatments for the turnover of the (13)C in the pancreas (T50% = 13.91, 14.37, 11.07, and 9.34 days; T95% = 46.22, 47.73, 36.79, and 31.04 days for CD, GD, GAD, and ND, respectively). In the liver, the ND presented accelerated values of carbon turnover (T50% = 7.36 and T95% = 24.47 days) in relation to the values obtained for the GD (T50% = 10.15 and T95% = 33.74 days). However, the values obtained for the CD (T50% = 9.12 and T95% = 30.31 days) and GAD (T50% = 7.83 and T95% = 26.03 days) had no differences (p>0.05) among other diets. The technique of (13)C isotopic dilution demonstrated trophic action of nucleotides in the liver.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5713/ajas.15.0132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4932569PMC
August 2016

Analysis of metabolic pools in broilers chicks.

Isotopes Environ Health Stud 2015 18;51(4):525-32. Epub 2015 Sep 18.

b Departamento de Física e Biofísica , Universidade Estadual Paulista (UNESP) , Botucatu , São Paulo , Brazil.

This paper shows the possibility of obtaining new parameters for the mathematical modelling of data on stable isotopes in biological systems and its application in obtaining data on metabolic pools of blood plasma, blood serum, liver and muscle of broilers. This theory states that the modelling of turnover used for studies of isotopic incorporation when the metabolism has a single metabolic pool is feasible by the technique of setting an exponential. However, when the metabolism has more than one metabolic pool, it is necessary to apply the linearization technique, linear regression adjustment and evaluation of the assumptions of regression to obtain the kinetic parameters such as half-life (T1/2) and isotope exchange rate (k). The application of this technique on carbon-13 data from 100 one-day-old chicks, with the change of diet composed of grains of the photosynthetic cycle of plants from C4 to C3, in broilers has enabled the discovery that the liver, blood plasma and blood serum have a single metabolic pool; however, the pectoral muscle has two metabolic pools. For the liver, blood plasma and blood serum, the half-life values were found by the exponential fit being T1/2 = 1.4 days with the rate of exchange of k = 0.502, T1/2 = 2.4 days with k = 0.293 and T1/2 = 2.0 days with k = 0.348, respectively. For the pectoral muscle, after linearization, the half-life values were found for T1/2(1) = 1.7 and T1/2(2) = 3 days, with exchange rates of k1 = 0.405 and k2 = 0.235, representing approximately 66 and 34%, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10256016.2015.1072529DOI Listing
September 2016

Does the rattle of Crotalus durissus terrificus reveal its dietary history?

J Venom Anim Toxins Incl Trop Dis 2014 9;20(1):53. Epub 2014 Dec 9.

Center for the Study of Venoms and Venomous Animals, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, São Paulo State Brazil ; Botucatu Medical School, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, São Paulo State Brazil ; CEVAP/UNESP, Rua José Barbosa de Barros, 1780, Fazenda Experimental Lageado, Botucatu, SP CEP 18610-307 Brazil.

Background: Environmental devastation threatens the survival of many species, including venomous snakes such as the South American rattlesnake Crotalus durissus terrificus. This observation is based on the decrease of snakes collected and donated to Brazilian research institutes. Nevertheless, some individuals have managed to survive and procreate. The question is how these snakes are adapting in these new environmental conditions.

Methods: To answer it, the carbon-13 level of rattlesnakes and their feed (either laboratory or wild mice) was evaluated by isotope-ratio mass spectrometry. Thus, rattle segments from 16 adults and 15 offspring of captive snakes, and of three wild newborn C. d. terrificus were evaluated as well as 17 Mus musculus mice captured in traps, four live feeder mice and the ration offered to mice at animal houses.

Results: The isotopic exchange time of the captive adult snakes (n = 16) varied between 33 and 37 months and of captive-born animals (n = 15), until reaching a plateau of equilibrium, varied from 18 to 24 months. Regarding the captured Mus musculus (n = 17), 88.23% (n = 15) were from a C4 environment. Of the six rattle rings from offspring of captured C. d. terrificus, five were from a C4 environment, whereas of the 170 rattle rings studied, 60% originated from a C3 environment and 40% from a C4. The same carbon-13 values were found in captive snakes.

Conclusions: Based on the present results, it can be inferred that most C. d. terrificus snakes (60%) fed animals from a C3 environment; birds consist of an alimentary alternative for snakes, as well as rodents, small reptiles and amphibians; different venom compositions among snakes from the same region may be related to the food type; the primary rattle of offspring reflects the maternal diet during gestation; and, finally, the different rattle rings indicate the alimentary history of these animals.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/1678-9199-20-53DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276105PMC
December 2014

Non-specific immune parameters and physiological response of Nile tilapia fed β-glucan and vitamin C for different periods and submitted to stress and bacterial challenge.

Fish Shellfish Immunol 2014 Aug 14;39(2):188-95. Epub 2014 May 14.

UNESP, Univ. Estadual Paulista, FMVZ, AquaNutri, Botucatu, SP, Brazil.

This study attempts to describe the effects of different administration periods of dietary β-glucan and Vit C on the non-specific immune response, physiological parameters and disease resistance of Nile tilapia against Aeromonas hydrophila infection. Therefore, a feeding trial (288 fish) was conducted to determine the best administration period (7, 15, 30 and 45 days) for a Nile tilapia diet supplemented with 0.1% β-glucan and 600 mg Vit C/kg diet. After the administration period, three different groups of 96 fish were exposed to one of the following three stresses: cold-induced stress, transport-induced stress, and A. hydrophila challenge. Hematological, biochemical and immunological responses were analyzed before and/or after stress. Cold-induced stress increased cortisol levels and reduced the leukocyte count in fish fed the test diet for seven days compared with the other periods. After transport-induced stress, fish fed the test diet for seven days required more hours to return to the baseline levels of cortisol and neutrophils. Moreover, independently of the administration period, fish needed 24 h for leukocyte and glucose levels to return to the initial values. The lowest survival after bacterial infection was observed in fish test diet for seven days. Based on fish hematological and biochemical responses, diet supplemented with 0.1% of β-glucan and 600 mg of Vit C/kg fed for at least 15 days is recommended for Nile tilapia especially when fish are likely to encounter transport-induced stress, and this stress was more severe than cold-induced stress or bacterial challenge.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fsi.2014.05.004DOI Listing
August 2014
-->