Publications by authors named "Maria L Rodgers"

12 Publications

  • Page 1 of 1

Galaxolide and tonalide modulate neuroendocrine activity in marine species from two taxonomic groups.

Environ Res 2021 Mar 4;196:110960. Epub 2021 Mar 4.

Physical Chemistry Department, University Institute of Marine Research (INMAR), International Campus of Excellence of the Sea (CEI.MAR), University of Cadiz, República Saharaui S/n, 11510, Puerto Real, Cádiz, Spain.

Galaxolide (HHCB) and tonalide (AHTN) are polycyclic musk compounds (PMCs) used in household and personal care products that have been included on the list as emerging contaminants of environmental concern due to their ubiquity in aquatic and terrestrial environments. There still exists a dearth of information on the neurotoxicity and endocrine disrupting effects of these contaminants, especially for marine and estuarine species. Here, we assessed the neuroendocrine effects of HHCB and AHTN using adult clams, Ruditapes philippinarum, and yolk-sac larvae of sheepshead minnow, Cyprinodon variegatus. The clams were treated with concentrations (0.005-50 μg/L) of each compound for 21 days. Meanwhile, sheepshead minnow larvae were exposed to 0.5, 5 and 50 μg/L of HHCB and AHTN for 3 days. Enzyme activities related to neurotoxicity (acetylcholinesterase - AChE), neuroendocrine function (cyclooxygenase - COX), and energy reserves (total lipids - TL) were assessed in R. philippinarum. Gene expression levels of cyp19 and vtg1 were measured in C. variegatus using qPCR. Our results indicated induction of AChE and COX in the clams exposed to HHCB while AHTN exposure significantly inhibited AChE and COX. Gene expression of cyp19 and vtg1 in yolk-sac C. variegatus larvae exposed to 50 μg/L AHTN was significantly downregulated versus the control. The results of this study demonstrate that HHCB and AHTN might pose neurotoxic and endocrine disrupting effects in coastal ecosystems.
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http://dx.doi.org/10.1016/j.envres.2021.110960DOI Listing
March 2021

Characterization of the differential expressed genes and transcriptomic pathway analysis in the liver of sub-adult red drum (Sciaenops ocellatus) exposed to Deepwater Horizon chemically dispersed oil.

Ecotoxicol Environ Saf 2021 May 2;214:112098. Epub 2021 Mar 2.

Mote Marine Laboratory, 1600 Ken Thompson Pkwy, Sarasota, FL 34236, USA.

The Deepwater Horizon blowout resulted in the second-largest quantity of chemical dispersants used as a countermeasure for an open water oil spill in the Gulf of Mexico. Of which, the efficacy of dispersant as a mitigation strategy and its toxic effects on aquatic fauna remains controversial. To enhance our understanding of potential sub-lethal effects of exposure to chemically dispersed-oil, sub-adult red drum (Sciaenops ocellatus) were continuously exposed to a Corexit 9500: DWH crude oil chemically enhanced water accommodated fraction (CEWAF) for 3-days and transcriptomic responses were assessed in the liver. Differential expressed gene (DEG) analysis demonstrated that 63 genes were significantly impacted in the CEWAF exposed fish. Of these, 37 were upregulated and 26 downregulated. The upregulated genes were primarily involved in metabolism and oxidative stress, whereas several immune genes were downregulated. Quantitative real-time RT-PCR further confirmed upregulation of cytochrome P450 and glutathione S-transferase, along with downregulation of fucolectin 2 and chemokine C-C motif ligand 20. Ingenuity Pathway Analysis (IPA) predicted 120 pathways significantly altered in the CEWAF exposed red drum. The aryl hydrocarbon receptor pathway was significantly activated, while pathways associated with immune and cellular homeostasis were primarily suppressed. The results of this study indicate that CEWAF exposure significantly affects gene expression and alters signaling of biological pathways important in detoxification, immunity, and normal cellular physiology, which can have potential consequences on organismal fitness.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112098DOI Listing
May 2021

Characterizing transcriptomic responses of southern flounder (Paralichthys lethostigma) chronically exposed to Deepwater Horizon oiled sediments.

Aquat Toxicol 2021 Jan 7;230:105716. Epub 2020 Dec 7.

Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, FL, 34236, United States.

To obtain a deeper understanding of the transcriptomic responses to oil in southern flounder (Paralichthys lethostigma), we performed quantitative PCR and RNA sequencing on liver and gill tissue after a chronic exposure (35 days) to Deepwater Horizon crude oiled sediment and after a 30-day recovery period. We wanted to understand which specific genes are differentially expressed in liver and gill tissues directly after oiled sediment exposure and with the addition of a recovery period. Furthermore, we wanted to examine specific enriched pathways in these two tissues to determine the impact of exposure with and without a recovery period on biological processes (e.g. immune function). Liver and gill tissues were chosen because they represent two distinct organs that are highly important to consider when examining the impacts of oiled sediment exposure. The liver is the classic detoxification organ, while the gill is in direct contact with sediment in benthic fishes. Examination of these two tissues, therefore, generates a broad understanding of the transcriptomic consequences of oil exposure across an organism. Gene expression for interleukin 8 (il8) and interleukin 1B (il1β) was significantly increased versus control measurements for fish exposed to oiled sediments for 35 days in gill tissue. Hierarchical clustering of gene expression showed that tissue type was the main driver of gene expression (rather than treatment). The inclusion of a 30-day post-exposure recovery period showed a return of il8 and il1β gene expression in the gill to baseline expression levels. However, the recovery period increased the number of differentially expressed genes and significantly affected canonical pathways in both tissue types. Pathways related to cholesterol biosynthesis were significantly suppressed in oil-exposed flounder with a recovery period, but not in the exposed flounder without a recovery period. At the end of the exposure, 17 pathways were significantly affected in the gill, including thyroid hormone metabolism-related pathways, which were the most influenced. Liver tissue from the recovered fish had the greatest number of enriched pathways for any tissue or time point (187). Cellular and humoral immune response pathways were considerably impacted in the liver after the recovery period, suggesting that the immune system was attempting to respond to potential damage caused from the chronic oil exposure. Our results demonstrate that liver and gill tissues from southern flounder were differentially altered by Deepwater Horizon oiled sediment exposure and that a 30-day recovery period after exposure substantially shifted gene expression and canonical pathway profiles.
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http://dx.doi.org/10.1016/j.aquatox.2020.105716DOI Listing
January 2021

The impact of salinity and dissolved oxygen regimes on transcriptomic immune responses to oil in early life stage Fundulus grandis.

Comp Biochem Physiol Part D Genomics Proteomics 2021 Mar 17;37:100753. Epub 2020 Nov 17.

Division of Coastal Sciences, School of Ocean Science and Engineering, University of Southern Mississippi, Ocean Springs, MS 39564, USA.

Understanding the effects of oil exposure on early life stage fish species is critical to fully assessing the environmental impacts of oil spills. Oil released from the 2010 Deepwater Horizon spill reached habitats where estuarine fish routinely spawn. In addition, estuaries are highly dynamic environments, therefore, fish in these areas are routinely exposed to varying salinity and dissolved oxygen (DO) levels, each of which are known to modulate transcriptional responses. Fish exposed to oil often display altered immune competence, and several studies have shown that Deepwater Horizon oil in particular causes modulation of various immune functions. However, few studies have directly examined how environmental parameters may affect oil-induced immunomodulation, particularly in early life stage fishes when the immune system is still developing. To this end, we examined transcriptional patterns of immune genes and pathways in Fundulus grandis larvae to various oil (0, 15 μg/L), salinity (3, 30 ppt), and DO (2.5, 6 mg/L) regimes in a fully factorial design. Our results suggest that immune pathways are generally activated in all treatment groups with the exception of the Low Salinity/No Oil/Hypoxia treatment where immune pathways are largely suppressed, and the High Salinity/No Oil/Hypoxia treatment where pathways are unchanged. The High Salinity/Oil/Hypoxia treatment had the largest number of enriched immune pathways (44 as defined by IPA and 43 as defined by ConsensusPathDB), indicating that oil under certain environmental conditions has the potential to further modulate immune-related genes, pathways, and responses in fish.
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http://dx.doi.org/10.1016/j.cbd.2020.100753DOI Listing
March 2021

Effects of polycyclic aromatic hydrocarbons and abiotic stressors on Fundulus grandis cardiac transcriptomics.

Sci Total Environ 2021 Jan 6;752:142156. Epub 2020 Sep 6.

Department of Forestry and Natural Resources, Purdue University, West Lafayette, IN, USA. Electronic address:

Following the 2010 Deepwater Horizon oil spill, extensive research has been conducted on the toxicity of oil and polycyclic aromatic hydrocarbons (PAHs) in the aquatic environment. Many studies have identified the toxicological effects of PAHs in estuarine and marine fishes, however, only recently has work begun to identify the combinatorial effect of PAHs and abiotic environmental factors such as hypoxia, salinity, and temperature. This study aims to characterize the combined effects of abiotic stressors and PAH exposure on the cardiac transcriptomes of developing Fundulus grandis larvae. In this study, F. grandis larvae were exposed to varying environmental conditions (dissolved oxygen (DO) 2, 6 ppm; temperature 20, 30 °C; and salinity 3, 30 ppt) as well as to a single concentration of high energy water accommodated fraction (HEWAF) (∑PAHs 15 ppb). Whole larvae were sampled for RNA and transcriptional changes were quantified using RNA-Seq followed by qPCR for a set of target genes. Analysis revealed that exposure to oil and abiotic stressors impacts signaling pathways associated with cardiovascular function. Specifically, combined exposures appear to reduce development of the systemic vasculature as well as strongly impact the cardiac musculature through cardiomyocyte proliferation resulting in inhibited cardiac function and modulated blood pressure maintenance. Results of this study provide a holistic view of impacts of PAHs and common environmental stressors on the cardiac system in early life stage estuarine species. To our knowledge, this study is one of the first to simultaneously manipulate oil exposure with abiotic factors (DO, salinity, temperature) and the first to analyze cardiac transcriptional responses under these co-exposures.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142156DOI Listing
January 2021

Publisher Correction: Exposure to Oil and Hypoxia Results in Alterations of Immune Transcriptional Patterns in Developing Sheepshead Minnows (Cyprinodon variegatus).

Sci Rep 2020 Mar 11;10(1):4851. Epub 2020 Mar 11.

Division of Coastal Sciences, School of Ocean Science and Engineering, University of Southern Mississippi, Ocean Springs, Mississippi, 39564, USA.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41598-020-61186-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066180PMC
March 2020

Exposure to Oil and Hypoxia Results in Alterations of Immune Transcriptional Patterns in Developing Sheepshead Minnows (Cyprinodon variegatus).

Sci Rep 2020 02 3;10(1):1684. Epub 2020 Feb 3.

Division of Coastal Sciences, School of Ocean Science and Engineering, University of Southern Mississippi, Ocean Springs, Mississippi, 39564, USA.

The area and timing of the Deepwater Horizon oil spill highlight the need to study oil and hypoxia exposure in early life stage fishes. Though critical to health, little research has targeted the effect of oil and hypoxia exposure on developing immune systems. To this end, we exposed sheepshead minnows (Cyprinodon variegatus) at three early life stages: embryonic; post-hatch; and post-larval, to a high energy water accommodated fraction (HEWAF) of oil, hypoxia, or both for 48 hours. We performed RNAseq to understand how exposures alter expression of immune transcripts and pathways. Under control conditions, the embryonic to post-hatch comparison (first transition) had a greater number of significantly regulated immune pathways than the second transition (post-hatch to post-larval). The addition of oil had little effect in the first transition, however, hypoxia elicited changes in cellular and humoral immune responses. In the second transition, oil exposure significantly altered many immune pathways (43), and while hypoxia altered few pathways, it did induce a unique signature of generally suppressing immune pathways. These data suggest that timing of exposure to oil and/or hypoxia matters, and underscores the need to further investigate the impacts of multiple stressors on immune system development in early life stage fishes.
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http://dx.doi.org/10.1038/s41598-020-58171-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997411PMC
February 2020

Deepwater Horizon oil alone and in conjunction with Vibrio anguillarum exposure modulates immune response and growth in red snapper (Lutjanus campechanus).

Aquat Toxicol 2018 Nov 8;204:91-99. Epub 2018 Sep 8.

Division of Coastal Sciences, School of Ocean Science and Technology, University of Southern Mississippi, Ocean Springs, MS, 39564, USA. Electronic address:

This study examined the impacts of Macondo oil from the Deepwater Horizon oil spill, both alone and in conjunction with exposure to the known fish pathogen Vibrio anguillarum, on the expression of five immune-related gene transcripts of red snapper (il8, il10, tnfa, il1b, and igm). In order to elucidate this impact, six different test conditions were used: one Control group (No oil/No pathogen), one Low oil/No pathogen group (tPAH50 = 0.563 μg/L), one High oil/No pathogen group (tPAH50 = 17.084 μg/L, one No oil/Pathogen group, one Low oil/Pathogen group (tPAH50 = 0.736 μg/L), and one High oil/Pathogen group (tPAH50 = 15.799 μg/L). Fish were exposed to their respective oil concentrations for one week. On day 7 of the experiment, all fish were placed into new tanks (with or without V. anguillarum) for one hour. At three time points (day 8, day 10, and day 17), fish organs were harvested and placed into RNAlater, and qPCR was run for examination of the above specific immune genes as well as cyp1a1. Our results suggest that cyp1a1 transcripts were upregulated in oil-exposed groups throughout the experiment, confirming oil exposure, and that all five immune gene transcripts were upregulated on day 8, but were generally downregulated or showed no differences from controls on days 10 and 17. Finally, both oil and pathogen exposure had impacts on growth.
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http://dx.doi.org/10.1016/j.aquatox.2018.09.002DOI Listing
November 2018

Monoclonal antibodies against loggerhead sea turtle, Caretta caretta, IgY isoforms reveal differential contributions to antibody titers and relatedness among other sea turtles.

Dev Comp Immunol 2018 10 19;87:12-15. Epub 2018 May 19.

Department of Biological Sciences, Clemson University, Clemson, SC, 29634, USA. Electronic address:

Serum from loggerhead sea turtles, Caretta caretta, was collected from the southeast Atlantic Ocean during routine summer monitoring studies in 2017. Serum immunoglobulin IgY was purified and used to develop IgY isoform-specific monoclonal antibodies (mAb). mAb LH12 was developed against the 66 kDa heavy chain of IgY, mAb LH1 was developed against the truncated heavy chain of approximately 37 kDA, and mAb LH9 was developed against the 23 kDa light chains. mAb LH9 reacts with the light chains of all sea turtles, mAb LH12 reacts with the long heavy chain of all sea turtles within the family Cheloniidae, and mAb LH1 reacts with the truncated form of IgY in both olive and Kemp's ridley turtles. Circulating IgY antibodies against three different marine bacterial pathogens were determined in 16 loggerhead samples using these mAbs. mAb LH12 detects higher titers than mAb LH1, and mAb LH9 detects the highest titers.
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http://dx.doi.org/10.1016/j.dci.2018.05.015DOI Listing
October 2018

Combined effects of Deepwater Horizon crude oil and environmental stressors on Fundulus grandis embryos.

Environ Toxicol Chem 2018 07 4;37(7):1916-1925. Epub 2018 Jun 4.

Department of Forestry and Natural Resources, Purdue University, West Lafayette, Indiana, USA.

In the present study, we examined how sensitivity to oil changes in combination with environmental stressors in Fundulus grandis embryos. We exposed embryos (<24 h post fertilization) to a range of high-energy water accommodated fraction (HEWAF) concentrations (0-50 parts per billion [ppb] total polycyclic aromatic hydrocarbons [PAHs]) made from Macondo crude oil in conjunction with various environmental conditions (temperature: 20 and 30 °C; salinity: 3, 7, and 30 practical salinity units [PSU]; and dissolved oxygen: 2 and 6 mg/L). Endpoints included mortality, hatching rates, and expression of cytochrome p450 1a and 1c (cyp1a, cyp1c) in hatched larvae. There was 100% mortality for all fish under the 2 parts per million (ppm) dissolved oxygen regimes. For the 6 mg/L dissolved oxygen treatments, mortality and median lethal time (LT50) were generally higher in the 30 °C treatments versus the 20 °C treatments. Oil increased mortality in fish exposed to the highest concentration in the 20-3-6 (°C-PSU-mg/L), 25-7-6, and 30-30-6 conditions. Hatching was driven by environmental conditions, with oil exposure having a significant impact on hatching in only the 25-7-6 and 30-30-6 groups at the greatest HEWAF exposure. Expression of cyp1a was up-regulated in most treatment groups versus the controls, with cyp1c expression exhibiting a similar pattern. These data suggest interactive effects among temperature, salinity, and PAHs, highlighting a need to further assess the effects of oil exposure under various environmental conditions. Environ Toxicol Chem 2018;37:1916-1925. © 2018 SETAC.
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http://dx.doi.org/10.1002/etc.4153DOI Listing
July 2018

Humoral Immune Responses to Select Marine Bacteria in Loggerhead Sea Turtles Caretta caretta and Kemp's Ridley Sea Turtles Lepidochelys kempii from the Southeastern United States.

J Aquat Anim Health 2018 03;30(1):20-30

Department of Biological Sciences, Clemson University, 132 Long Hall, Clemson, South Carolina, 29634, USA.

Serum from Kemp's ridley sea turtles Lepidochelys kempii and loggerhead sea turtles Caretta caretta was collected during summer in 2011, 2012, and 2013. Serum immunoglobulin Y (IgY) recognition of lysate proteins from nine bacterial species and whole bacterium-specific IgY titers to these pathogens were quantified. Serum and purified IgY recognized proteins of all bacteria, with protein recognition for some species being more pronounced than others. Circulating IgY titers against Vibrio vulnificus, V. anguillarum, Erysipelothrix rhusiopathiae, and Brevundimonas vesicularis changed over the years in Kemp's ridley sea turtles, while IgY titers against V. vulnificus, Escherichia coli, V. parahaemolyticus, B. vesicularis, and Mycobacterium marinum were different in loggerhead sea turtles. Serum lysozyme activity was constant for loggerhead sea turtles over the 3 years, while activity in Kemp's ridley sea turtles was lower in 2011 and 2012 than in 2013. Blood packed cell volume, glucose, and serum protein levels were comparable to those of healthy sea turtles in previous studies; therefore, this study provides baseline information on antibody responses in healthy wild sea turtles.
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http://dx.doi.org/10.1002/aah.10012DOI Listing
March 2018

Humoral immune responses to select marine bacteria in loggerhead, Caretta caretta, and Kemp's ridley, Lepidochelys kempii, sea turtles from the south eastern United States.

J Aquat Anim Health 2017 Oct 12. Epub 2017 Oct 12.

a Clemson University , Clemson , SC , USA.

Serum from Kemp's ridley and loggerhead sea turtles was collected in summers of 2011, 2012, and 2013. Serum IgY recognition of lysate proteins from nine bacteria species and whole bacteria-specific IgY titers to these pathogens were quantified. Serum and purified IgY recognized proteins of all bacteria, with protein recognition in some species more pronounced than others. Circulating IgY titers against V. vulnificus, V. anguillarum, E. rhusiopathiae, and B. vesicularis changed over the years in Kemp's ridley turtles, while titers IgY against V. vulnificus, E. coli, V. parahaemolyticus, B. vesicularis, and M. marinum were different in loggerhead turtles. Serum lysozyme activity was constant for loggerhead turtles over the 3 years, while activity in Kemp's ridleys were lower in 2012 than 2013. Blood PCV, glucose levels, and serum protein levels were comparable to healthy turtles in previous studies, therefore, this study provides baseline information on antibody responses in healthy, wild turtles. Received 25 May 2017 accepted 09 Oct 2017 revised 15 Sep 2017.
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http://dx.doi.org/10.1080/08997659.2017.1391887DOI Listing
October 2017