Publications by authors named "Margaret Wanjiku Muchiri"

2 Publications

  • Page 1 of 1

Comparative pathogenicity of Trypanosoma brucei rhodesiense strains in Swiss white mice and Mastomys natalensis rats.

Acta Trop 2015 Oct 19;150:23-8. Epub 2015 Jun 19.

Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Kenya.

We evaluated Mastomys natelensis rat as an animal model for Rhodesian sleeping sickness. Parasitaemia, clinical and pathological characteristics induced by T. b. rhodesiense isolates, KETRI 3439, 3622 and 3637 were compared in Mastomys rats and Swiss white mice. Each isolate was intra-peritonially injected in mice and rat groups (n=12) at 1×10(4) trypanosomes/0.2mL. Pre-patent period (PP) range for KETRI 3439 and KETRI 3622-groups was 3-6 days for mice and 4-5 days for rats while for KETRI 3637-infected mice and rats was 5-9 and 4-12 days, respectively. Pairwise comparison between PP of mice and rats separately infected with either isolate showed no significant difference (p>0.05). The PP's of KETRI 3637-infected mice were significantly (p>0.01) longer than those infected with KETRI 3439 or KETRI 3622, a trend also observed in rats. The second parasitaemic wave was more prominent in mice. Clinical signs included body weakness, dyspnoea, peri-orbital oedema and extreme emaciation which were more common in rats. Survival time for KETRI 3439 and 3622-infected groups was significantly (p<0.05) longer in mice than rats but similar in KETRI 3637-infected groups. Inflammatory lesions were more severe in rats than mice. All mice and KETRI 3622-infected rats had splenomegaly, organ congestion with rats additionally showing prominent lymphadenopathy. KETRI 3439-infected rats showed hemorrhagic pneumonia, enteritis with moderate splenomegaly and lymphadenopathy. KETRI 3637-infected rats had the most severe lesions characterized by prominent splenomegaly, lymphadenopathy, hepatomegaly, enlarged adrenal glands, organ congestion, generalized oedemas, gastroenteritis, pneumonia and brain congestion. KETRI 3637-infected Mastomys is a suitable model for studying pathophysiology of HAT.
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October 2015

Effects of Ethidium (homidium bromide) on female reproductive performance of laboratory-reared tsetse flies, Glossina morsitans morsitans Westwood (Diptera: Glossinidae).

Acta Trop 2010 Nov 1;116(2):157-60. Epub 2010 Aug 1.

Kenya Agricultural Research Institute-Trypanosomiasis Research Centre (KARI-TRC), Kikuyu, Kenya.

Ethidium (homidium bromide) is a trypanocide likely to be encountered as a violative residue in blood collected from abattoirs for feeding laboratory tsetse flies. We investigated its effect on female reproduction of Glossina morsitans morsitans. One-milligram homidium per kilogram body weight was intra-muscularly administered to four steers and blood aseptically collected from them between 15 and 30 min post-treatment, analysed for homidium levels and processed for tsetse feeding. Two hundred teneral female flies were fed on homidium-treated diet while a control group of similar number was given untreated diet and the reproductive performance of the two groups statistically compared. Ethidium, at 266.1 ng homidium/ml blood diet, halved A-class portion of F(1)-pupae, highly reduced decline of F(1)-progeny quality associated with aging parents, but had no significant effect on the pupae viability, fecundity and abortion rate of the flies. We therefore concluded that Ethidium has beneficial effect on laboratory tsetse attributable to clearance of unfavourable microbes mediated by the drug, and could be used as a tsetse diet additive.
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November 2010