Publications by authors named "Marek Lipiński"

26 Publications

  • Page 1 of 1

Prevalence and bother of lower urinary tract symptoms and overactive bladder in Poland, an Eastern European Study.

Sci Rep 2020 11 13;10(1):19819. Epub 2020 Nov 13.

Department of Urology, Jagiellonian University Medical College, Krakow, Poland.

The prevalence of lower urinary tract symptoms (LUTS) and overactive bladder (OAB) has been measured by population-based investigations in many parts of the world. However, data are lacking for Eastern Europe, and there has not been any large population-representative study in any country of this region. Therefore, the aim of this study was to evaluate the prevalence and associated bother of LUTS and OAB in a population-representative sample of persons aged ≥ 40 years in Poland. This investigation was conducted as a computer-assisted telephone interview. The survey sample was stratified by age, sex, and place of residence to reflect the entire Polish population. LUTS and OAB were assessed by a standardized protocol based on the International Continence Society definitions and validated questionnaires. Of 6005 participants, 57% were women, and the mean age (range) was 60.7 (40-93) years. The prevalence of LUTS was 69.8% (men 66.2%; women 72.6%). There was no difference in prevalence between urban and rural areas. LUTS were often bothersome among men and women, but women were more likely to be bothered compared with men. There were also statistically significant correlations between the frequency and the bother intensity of each of the LUTS. The prevalence of OAB was higher in women (39.5%) than in men (26.8%), and OAB increased with age. Lastly, LUTS had detrimental effects on the quality of life because one third of the participants had concerns about their urinary-specific quality of life. This investigation was the first nationwide, population-representative epidemiological study of LUTS and OAB in an Eastern-European country. LUTS were highly prevalent, often bothersome, and had negative effects on the quality of life of men and women aged ≥ 40 years. Our findings are comparable with other epidemiologic studies of LUTS and OAB conducted in different regions of the world.Trial registration: NCT04121936.
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http://dx.doi.org/10.1038/s41598-020-76846-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666180PMC
November 2020

The correlation of crystalline and elemental composition of urinary stones with a history of bacterial infections: TXRF, XRPD and PCR-DGGE studies.

Eur Biophys J 2019 Jan 27;48(1):111-118. Epub 2018 Nov 27.

2nd Department of Urology, Medical University of Lodz, Pabianicka St. 62, 93-513, Łódź, Poland.

The aim of this study was to analyze the correlation between past bacterial infections and the type and chemical composition of urinary stones experienced by human patients. Bacteria have been recognized to contribute to urinary stones; however, the role of uropathogens in the development of specific stones has not been extensively investigated. The detection of past bacterial infection (eleven different bacterial species) in urinary stones from 83 patients was made on a DNA level using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) and correlated with the chemical composition of urinary stones measured using X-ray powder diffraction (XPRD) technique and their elemental composition by total reflection X-ray fluorescence (TXRF). In this study, two scenarios of urinary stones formation mediated by Proteus sp. or Escherichia coli are presented. The first one is associated with Proteus spp. which dominated in 84% of infectious urinary stones and is strongly correlated with struvite and calcium phosphate, in whose matrix additionally strontium, phosphorus, potassium, nickel and zinc are detected. The formation of these stones is closely correlated with urease activity. The second scenario for urinary stone mineralization is associated with E. coli identified in weddellite stones, in which matrix iron was detected. In conclusion, the statistical correlations of bacterial infections with crystalline and elemental composition showed that in mixed bacterial infections, one scenario dominated and excluded the second one.
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http://dx.doi.org/10.1007/s00249-018-1338-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330562PMC
January 2019

Diagnostic and therapeutic recommendations for patients with nocturia.

Cent European J Urol 2017 25;70(4):388-393. Epub 2017 Sep 25.

Department of Urology, Jagiellonian University, Medical College, Cracow, Poland.

Introduction: Nocturia is defined as the urge to urinate at night when the micturition was directly preceded and followed by sleep. Due to its negative impact on the quality of life of patients, an effective treatment for this disease has become a significant therapeutic challenge. The aim of this article was to explain the main risk factors for the occurrence of nocturia and to present diagnostic and therapeutic schemes in the case of nocturnal polyuria (idiopathic night time polyuria).

Material And Methods: A review of the literature was carried out and the available guidelines of international science societies, which provided the basis for the above recommendations, were analyzed.

Results: Detailed medical history should include information concerning: lower urinary tract symptoms (including nocturia), underlying illnesses, urogenital disorders, previous surgeries and medications administered. Keeping a bladder diary is recommended. The physical examination, depending on the patient's gender, should include gynecological examination with pelvic organ prolapse assessment or prostate evaluation. In laboratory tests, a urinalysis may be used, in particular cases a cytological analysis of urine sediment may be carried out. In addition, a possible ultrasound and/or cystoscopy may be conducted. Nocturia therapy should begin with modifying dietary habits, including compliance with the fluid regimen, avoiding alcohol, coffee and tea. Moderate physical exercise is also recommended. The pharmacological treatment of nocturia caused by nocturnal polyuria is based on the use of desmopressin at a daily single dose of 25 µg for women and 50 µg for men. The use of desmopressin allows for the reduction in the number of nocturia episodes, as well as improves the overall quality of life and sleep. Treatment with desmopressin can also be considered as a form of therapy added in people with an overactive bladder or benign prostatic hyperplasia, in which nocturia is a significant clinical problem.

Conclusions: Desmopressin is an effective and safe first-line treatment option in pharmacological therapy of nocturia caused by nocturnal polyuria.
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http://dx.doi.org/10.5173/ceju.2017.1563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5791408PMC
September 2017

Endoscopic extraperitoneal radical prostatectomy: An initial report following the first 30 cases.

Cent European J Urol 2017 14;70(1):48-52. Epub 2017 Mar 14.

2 Department of Urology, Medical University of Łódź, Łódź, Poland.

Introduction: To present initial observations after the first 30 cases of endoscopic extraperitoneal radical prostatectomy carried out at our department, which so far has had no experience with this surgical procedure.

Material And Methods: In the period of 15 months a group of 30 patients with organ confined prostate cancer, underwent endoscopic extraperitoneal radical prostatectomy using Montsouris technique. All procedures were performed by the same team of two urologists and one resident.

Results: The mean age of the patients was 65.3 years (43-73 years), the mean preoperative prostate specific antigen (PSA) was 7.2 ng/ml (4-9.8 ng/ml), the mean prostate volume measured in TRUS was 41 cm³ (25-80 cm³). The mean operative time was 3 h 55 min (3 h 15 min - 5 h 30 min). The negative margin was achieved in 26 patients (86%). In seven patients (23%) blood transfusion was required. Three patients had intraoperative rectal injury. In two cases trauma was supplied laparoscopically, and in one case it was decided to perform diverting colostomy. The majority of patients (65%) were discharged home on the fifth day after surgery. Two months postoperatively 13 patients (43%) were continent, 16 (35%) presented moderate stress incontinence with occasional urine leakage during normal activity and 1 patient (3%) presented severe stress incontinence.

Conclusions: Endoscopic extraperitoneal radical prostatectomy during the early phase of learning is technically difficult, requiring from the operator the laparoscopic skills, determination and a thorough knowledge of the theoretical basis of the subsequent stages of the procedure. Urologists who start performing this procedures must be aware of possible intra as well as postoperative complications.
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http://dx.doi.org/10.5173/ceju.2017.829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5407326PMC
March 2017

Spatio-temporal genetic structure and the effects of long-term fishing in two partially sympatric offshore demersal fishes.

Mol Ecol 2016 Dec 11;25(23):5843-5861. Epub 2016 Nov 11.

Evolutionary Genomics Group, Department of Botany and Zoology, Stellenbosch University, Private Bag X1, Stellenbosch, 7602, South Africa.

Environmental gradients have been shown to disrupt gene flow in marine species, yet their influence in structuring populations at depth remains poorly understood. The Cape hakes (Merluccius paradoxus and M. capensis) are demersal species co-occurring in the Benguela Current system, where decades of intense fishing resulted in severely depleted stocks in the past. Previous studies identified conflicting mtDNA genetic substructuring patterns and thus contrasting evolutionary trajectories for both species. Using 10 microsatellite loci, the control region of mtDNA and employing a seascape genetics approach, we investigated genetic connectivity and the impact of prolonged exploitation in the two species, which are characterized by different patterns of fishing pressure. Three consecutive years were sampled covering the entire distribution (N = 2100 fishes). Despite large estimated population sizes, both species exhibited low levels of contemporary genetic diversity (0.581 < H  < 0.692), implying that fishing has had a significant impact on their genetic composition and evolutionary trajectories. Further, for M. paradoxus, significant temporal, but not spatial, divergence points to the presence of genetic chaotic patchiness. In contrast, M. capensis exhibited a clear latitudinal cline in genetic differentiation between Namibia and South Africa (F  = 0.063, P < 0.05), with low (0.2% per generation) estimates of contemporary gene flow. Seascape analyses reveal an association with bathymetry and upwelling events, suggesting that adaptation to local environmental conditions may drive genetic differentiation in M. capensis. Importantly, our results highlight the need for temporal sampling in disentangling the complex factors that impact population divergence in marine fishes.
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http://dx.doi.org/10.1111/mec.13890DOI Listing
December 2016

Sensitivity of neoplastic cells to senescence unveiled under standard cell culture conditions.

Anticancer Res 2015 May;35(5):2759-68

Department of Tumor Biology, Medical University of Lodz, Lodz, Poland.

Background: Cancer cells are typically defined as infinitely proliferating, whereas normal cells (except stem cells) are considered as being programmed to become senescent. Our data show that this characterization is misleading.

Materials And Methods: Multiplex Ligation-dependent Probe Amplification, TP53 sequencing, real-time polymerase chain reaction (PCR) for MUC1 and SCGB2A2 and immunocytochemistry, together with senescence detection assay and real-time microscopic observations were used to analyze primary neoplastic cells isolated from prostate, breast and colorectal tumors, as well as stable cancer cell lines (MCF7, MDA-MB-468, SW962, SK-MEL28, NCI-H1975 and NCI-H469).

Results: In all cases of primary cancer cell cultures, in vitro conditions rapidly revealed senescence in the majority of cells. Two out of six stable cancer cell lines did not exhibit any senescence-associated-β-Galactosidase-positive cells. Interestingly, four cell lines had small sub-populations of senescent cells (single SA-β-Gal-positive cells).

Conclusion: Primary neoplastic cells from different types of cancer (prostate, breast, colon cancer) appear to be senescent in vitro. Apparently, cancer cell lines that have been used for many years in drug-testing analyses have constantly been misleading researchers in terms of the general sensitivity of cancer cells to senescence.
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May 2015

[Chromophobe renal cell carcinoma - case report].

Pol Merkur Lekarski 2015 Feb;38(224):100-3

Department of General, Oncologic and Functional Urology - II Department of Urology, Medical University of Łódź, Poland.

Malignant tumors of the kidney, which the most common is renal cell carcinoma (RCC) is diagnosed in Poland in more than 5,000 patients each year. Most cases of kidney cancer occurs after the age of 55 years. In men, the risk is 2 times higher than in women. Among the various histological subtypes of RCC, 5% of cases of chromophobe renal cell carcinoma (chRCC). The 1% is in combination with oncocytoma, creating a hybrid chromophobe renal cell carcinoma. The paper presents a case report of a patient operated on because of a kidney tumor - eosinophilic type of chromophobe cancer. During subsequent care of patients experienced a rare complication of this type of tumor, ie. metastasized to the paraaortic lymph nodes. Another surgery and radiotherapy were later stages of treatment. Discussed in the paper example of a patient with type eosinophilic chRCC indicate the variable nature and mileage as compared with typical of the tumor, thus requiring increased surveillance oncology. This requires a careful approach clinicians at the stage of diagnosis and then treatment and aftercare.
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February 2015

Alternating expression levels of tumor suppressor and cancer-related genes in patients with bladder cancer.

Oncol Lett 2014 Nov 22;8(5):2291-2297. Epub 2014 Aug 22.

Department of Molecular Cancerogenesis, Medical University of Lodz, Lodz 90-752, Poland.

The aim of the present study was to determine the roles of the tumor suppressor and cancer-related genes in bladder tumor carcinogenesis. Reverse transcription-quantitative polymerase chain reaction was used to analyze the status of promoter methylation (using MethylScreen™ technology) and loss of heterozygosity (LOH) in papillary urothelial cancer tissues. The associations between the expression levels of the following tumorigenesis-related genes were also assessed: The tumor suppressor gene the proliferation gene, the and apoptotic genes, the signal transduction gene, the vascular endothelial growth factor gene, and the and cell cycle genes. The results reveal a high frequency of LOH in intron 1 in the gene, as well as an association between reduced expression levels and increased promoter methylation. In addition, the present study demonstrates that in bladder tumors, apoptosis is inhibited by increased expression levels of the gene. A correlation between the proliferation indices of the and the genes was also revealed Furthermore, the expression levels of were identified to be positively associated with those of the gene.
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http://dx.doi.org/10.3892/ol.2014.2476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186597PMC
November 2014

The evaluation of tissue mass loss in the incision line of prostate with benign hyperplasia performed using holmium laser and cutting electrode.

Cent European J Urol 2014 18;67(3):282-6. Epub 2014 Aug 18.

2 Clinic of Urology, Medical University of Łódź, Poland.

Introduction: The aim of this study is to compare the changes in the incision line of prostatic adenoma using a monopolar cutting electrode and holmium laser, as well as the assessment of associated tissue mass and volume loss of benign prostatic hyperplasia (BPH).

Material And Methods: The material used in this study consisted of 74 preparations of prostatic adenoma obtained via open retropubic adenomectomy, with an average volume of 120.7 ml. The material obtained cut in vitro before fixation in formaldehyde. One lobe was cut using holmium laser, the other using a monopolar cutting electrode. After the incision was made, tissue mass and volume loss were evaluated. Thermocoagulation changes in the incision line were examinedunder light microscope.

Results: In the case of the holmium laser incision, the average tissue mass loss was 1.73 g, tissue volume loss 3.57 ml and the depth of thermocoagulation was 1.17 mm. When the monopolar cutting electrode was used average tissue mass loss was 0.807 g, tissue volume loss 2.48 ml and the depth of thermocoagulation was 0.19 mm.

Conclusions: Where holmium laser was used, it was observed that the layer of tissue with thermocoagulation changes was deeper than in the case of the monopolar cutting electrode. Moreover, it was noticed that holmium laser caused bigger tissue mass and volume loss than the cutting electrode.
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http://dx.doi.org/10.5173/ceju.2014.03.art14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4165671PMC
September 2014

Rate of positive urine culture and double-J catheters colonization on the basis of microorganism DNA analysis.

Cent European J Urol 2014 17;67(1):81-5. Epub 2014 Apr 17.

2nd Department of Urology, Medical University of Łódź, Łódź, Poland.

Introduction: The aim of the trial was to estimate the relationship between colonization of the Double-J catheter, and the microorganisms cultured from urine.

Material And Methods: 60 patients, who had Double-J catheters inserted, participated in the study. All the subjects had their midstream urine samples taken prior to the stent insertion and removal. A negative urine culture before catheterization was mandatory to participate in the study. The patients were assigned into three subgroups, according to stenting duration: 1) 20 to 30 days (18 cases); 2) 30 to 90 days (30 cases); 3) longer than 90 days (12 cases). Bacterial and fungal DNA was identified using electrophoresis in polyacrylamide gel with a denaturing gradient (PCR-DGGE). The relationship between the genetic analysis of the catheter and the urine culture was estimated.

Results: Urine cultures were positive in only 8 patients, while Double-J catheter analyses were positive in all cases. In 2 cases one type of microorganism was isolated from the stent surface while the remaining 58 catheters were colonized by more than one pathogen. In three cases fungi were isolated. There were only three types of pathogens cultured from urine specimens. Urine and stent cultures were consistent in 5 cases. In 3 cases urine culture and stent analysis were not consistent.

Conclusions: Double-J catheter retention in the urinary tract is associated with an extremely high risk of bacterial colonization, while the risk of urine infection is about 8-fold lower. There is a great inconsistency between urine infection and catheter colonization, indicating a low predictive value of urine culture for estimating stent colonization.
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http://dx.doi.org/10.5173/ceju.2014.01.art18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074722PMC
July 2014

Environmental effects on cephalopod population dynamics: implications for management of fisheries.

Adv Mar Biol 2014 ;67:99-233

Department of Ichthyology and Fisheries Science, Rhodes University, Grahamstown, South Africa.

Cephalopods are a relatively small class of molluscs (~800 species), but they support some large industrial scale fisheries and numerous small-scale, local, artisanal fisheries. For several decades, landings of cephalopods globally have grown against a background of total finfish landings levelling off and then declining. There is now evidence that in recent years, growth in cephalopod landings has declined. The commercially exploited cephalopod species are fast-growing, short-lived ecological opportunists. Annual variability in abundance is strongly influenced by environmental variability, but the underlying causes of the links between environment and population dynamics are poorly understood. Stock assessment models have recently been developed that incorporate environmental processes that drive variability in recruitment, distribution and migration patterns. These models can be expected to improve as more, and better, data are obtained on environmental effects and as techniques for stock identification improve. A key element of future progress will be improved understanding of trophic dynamics at all phases in the cephalopod life cycle. In the meantime, there is no routine stock assessment in many targeted fisheries or in the numerous by-catch fisheries for cephalopods. There is a particular need for a precautionary approach in these cases. Assessment in many fisheries is complicated because cephalopods are ecological opportunists and stocks appear to have benefited from the reduction of key predator by overexploitation. Because of the complexities involved, ecosystem-based fisheries management integrating social, economic and ecological considerations is desirable for cephalopod fisheries. An ecological approach to management is routine in many fisheries, but to be effective, good scientific understanding of the relationships between the environment, trophic dynamics and population dynamics is essential. Fisheries and the ecosystems they depend on can only be managed by regulating the activities of the fishing industry, and this requires understanding the dynamics of the stocks they exploit.
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http://dx.doi.org/10.1016/B978-0-12-800287-2.00002-0DOI Listing
September 2014

Rare case of malignant lymphoma of kidney.

Cent European J Urol 2013 18;66(3):296-8. Epub 2013 Nov 18.

2nd Department of Urology, Medical University of Łódź, Poland.

We present a 64-year-old patient suffering from extranodal malignant lymphoma. The patient was admitted to the County Hospital Urology Ward with suspicion of the left kidney tumor. As part of pre-operational diagnosis, ultrasonographic examination and computed tomography of kidneys were conducted. The results confirmed the initial diagnosis. After undergoing radical surgical treatment, the patient is currently under the care of hematology ward of oncology centre.
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http://dx.doi.org/10.5173/ceju.2013.03.art11DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974471PMC
April 2014

Prediction of bladder cancer based on urinary content of MGEA5 and OGT mRNA level.

Clin Lab 2012 ;58(5-6):579-83

2nd Department of Urology, Medical University of Lodz, Lodz, Poland.

Background: The mRNA expression of genes coding enzymes involved in O-GlcNAcylation were analyzed in urine obtained from 176 bladder cancer (BC) patients and 143 healthy persons.

Methods: MGEA5 and OGT expression was measured by a real-time PCR assay.

Results: OGT expression was not detected in urine of healthy persons but it was found in 51.7% of BC samples. Positive expression of MGEA5 was found in urine of both healthy persons (47.1%) and BC patients (52.3%). Poorly differentiated BC (grade III) showed significantly lower MGEA5 expression than grade I tumors. Contrary, OGT transcript level was significantly higher in grade II and III in comparison to grade I BC. Moreover, there was significant difference in OGT expression between early bladder cancers and invasive or advanced bladder cancers.

Conclusions: These results suggest that analysis of urinary content of MGEA5 and OGT may be useful for bladder cancer diagnostics.
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July 2012

HIF-1, GLUT1, endoglin, and BIRC5 expression in urine samples obtained from patients with bladder malignancies - after photodynamic diagnosis (PDD).

Cent European J Urol 2012 4;65(3):146-50. Epub 2012 Sep 4.

Clinic of Urology and Urological Oncology, Medical University of Łódź, Copernicus Memorial Hospital, Łódź, Poland.

Introduction: Bladder cancer (BC) is a serious medical problem. The high rate of recurrence and progression demands the development of new methods, such as genetic markers, which allow diagnosis and patient follow-up.

Objectives: The aim of this study was to compare expression of HIF-1, GLUT1, endoglin, and BRIC5 in patients without and those with BC. The second group was divided into sub-groups: those without a history of PDD (photodynamic diagnosis) in the diagnostic process and those after PDD.

Methods: Patients with BC were diagnosed using the PDD method using hexaminolevulinate (Hexvix(®)). The expressions of HIF-1, GLUT1, endoglin, and BRIC5 genes were established in urine specimens by real-time quantitative polymerase chain reaction (PCR).

Results: The expressions of all tested genes were higher in the group of patients with BC than in the group without BC. In the group after PDD, a statistically significant overexpression of HIF-1 was observed. In this group, changes were not observed in cases of the other three tested genes.

Conclusions: The differences between the group with PDD and the group without it can be connected with the direct influence of PDD on malignant tissue, which can cause overexpression of HIF-1 only. This is, however, only a hypothesis and needs further study.
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http://dx.doi.org/10.5173/ceju.2012.03.art10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921788PMC
February 2014

Complication rates after prostate biopsy according to the number of sampled cores.

Cent European J Urol 2012 4;65(3):116-8. Epub 2012 Sep 4.

2 Department of Urology, Medical University of Łódź, Poland.

Introduction: A PROSTATE BIOPSY CAN RESULT IN SUCH COMPLICATIONS AS: hematuria, rectal bleeding, pain in hypogastrium, perineum or urethra, fever, nausea, vomiting, retention of urine or other adverse events. The aim of this research was to estimate complication rates after a prostate biopsy based on the number of cores.

Material And Methods: The complication rate was evaluated on the basis of questionnaires filled out by patients. Questions were related to the occurrence of mentioned complications on the first and second day after prostate biopsy. Patients were divided into two groups: 1(st) group (41 patients) 5-8 cores and 2(nd) group (73 patients) 12 or more cores.

Results: There was no significant statistical difference in the occurrence of complications mentioned in the questionnaires in both groups. The biggest difference was recorded for hematuria - 1(st) day: 39% in the 1st and 53% in the 2nd group (p = 0.1398); 2(nd) day: 15% in the 1(st) and 30% in the 2(nd) group (p = 0.0650). Rectal bleeding on the 1(st) day also seems to vary: 12% in the 1st and 26% in the 2(nd) group (p = 0.0835). Other complications occurred in 3-8% of patients. 32% of patients in the 1(st) and 29% in the 2(nd) group (p = 0.7419) had no complications at all.

Conclusions: The most common complications after a prostate biopsy are hematuria and rectal bleeding. Other complication rates are low. In general, complication rates after a prostate biopsy procedure are not related to the number of sampled cores.
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http://dx.doi.org/10.5173/ceju.2012.03.art3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921797PMC
February 2014

Selected examples of complications after minimally invasive treatment for urolithiasis.

Cent European J Urol 2012 12;65(2):80-3. Epub 2012 Jun 12.

2 Department of Urology, Medical University of Łódź, Łódź, Poland.

In recent years urologists have concentrated on the intense introduction of minimally invasive methods for the treatment of urinary tract diseases with major progress noted in the treatment of urolithiasis. Nowadays extracorporeal shockwave lithotripsy (ESWL), percutaneous nephrolithotomy (PCNL), and ureteroscopic lithotripsy (URSL) are widely used in the treatment of urinary tract lithiasis. The aim of this study is to present examples of urinary tract lithiasis as the complication after minimally invasive methods used in the treatment of urolithiasis. One should remember that even minimally invasive medical procedures using the instruments retained in long-term contact with urine may be the cause of incrustation and stone formation.
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http://dx.doi.org/10.5173/ceju.2012.02.art5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921780PMC
February 2014

Semen quality in men from subfertile couples from the region of Łódź (Poland) according to the new reference values recommended by WHO 2010.

Cent European J Urol 2011 18;64(1):34-8. Epub 2011 Mar 18.

Department of Andrology and Reproductive Endocrinology, Medical University of Łódź, Poland.

The semen analysis is the main diagnostic tool for evaluating the male fertility potential. The standard semen analysis includes evaluation of the sperm concentration, motility, and their morphology. The most important question is whether the results from semen analysis may be accurate markers for male fertility. Therefore, we retrospectively studied sperm quality among men attending the infertility clinic due to reproductive problems consistent with the WHO manual from 1999, which were reassessed according to the manual from 2010. Semen results from 571 males from couples undergoing fertility investigation were analyzed. All subjects included in the study had no abnormalities during examination. In 64 samples (11.2%), a leukocyte count above 1 x 10(6)/ml was found and their semen volume (median 3.2 ml) was significantly lower in comparison with the group without leukocytes (3.6 ml; p <0.001). Normal semen parameters were found in 290 subjects (50.8%) according to the 1999 manual and in 362 men (63.4%) according to the 2010 manual. The normozoospermia group, according to the 2010 manual, had a significantly lower percentage of sperm with progressive motility, motile sperm concentration, and total number of motile sperm in comparison with the normozoospermia group according to the manual from 1999. It seems that routine semen analysis is not sufficient to estimate male fertility potential and some men with normal semen parameters may be subfertile. Further investigations are needed.
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http://dx.doi.org/10.5173/ceju.2011.01.art7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921700PMC
February 2014

Comparison of the effectiveness of crushing concrements in the urinary tract with the use of holmium laser and sonotrode.

Cent European J Urol 2011 18;64(1):26-9. Epub 2011 Mar 18.

2 Clinic of Urology, Department of Urology, Medical University, Łódź, Poland.

Introduction: Holmium lasers and ultrasound probes are widely used in urinary stone lithotripsy. The authors present a comparison of both methods in urinary stones lithotripsy.

Materials And Methods: We analyzed 164-patients who underwent endoscopic lithotripsy. Ninety-eight of them (group I) were treated with the holmium laser Omni-Pulse Max 80 and were compared to the other 66-patients (group II) who were operated on with an ultrasound probe (sonotrode). Prior to the procedures, all patients were exposed to urological ultrasound and radiological imaging in order to localize the stone. The state where the stones were invisible in the radiological and renal ultrasound imaging that was performed after the operation was considered to be effective. Patients were operated under intravenous general or subepidural anesthesia.

Results: The effectiveness of laser lithotripsy in the bladder amounted to 100% (25 of 25 patients). In the case of stones localized in the ureter it reached 89%. The total effectiveness of the procedure reached 92% (90 out of 98 procedures). In group II, the total effectiveness of the procedure reached 79% (52 out of 66 procedures). In group I, two cases the inflammatory changes of the ureter made the endoscopy impossible. These two patients were operated with open surgery. In one case, the laser lithotripsy was complicated with a perforation of the ureter.

Conclusion: The results prove that laser lithotripsy is a method of high effectiveness with a low risk of complication. It might surpass sonotrode and become its alternative. Both methods have both advantages and disadvantages.
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http://dx.doi.org/10.5173/ceju.2011.01.art5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921703PMC
February 2014

[EORTC risk tables--a new diagnostic tool in urology].

Pol Merkur Lekarski 2009 Dec;27(162):524-8

Uniwersytet Medyczny w Łodzi, II Klinika Urologii.

75-85% newly diagnosed bladder cancers are lesions that are not invading the muscle layer of bladder. Patients treated for bladder cancer in this stage are characterized that part of them will develop recurrent bladder cancer and it may progress. Depending on a patient's characteristics, after transurethral resection (TUR), the probability of recurrence at one year ranges from about 15% to 70% and the probability of progression at five years ranges from about 7% to 40%. Non-muscle invasive bladder cancer is a heterogeneous group of tumors with completely different oncological outcome. Taking into consideration the risk of recurrence and progression, many researchers attempted to define risk factors for bladder cancer. After many test and researches had been conducted in different research and development centres a few risk factors were detected such as: number of tumors, tumor size, recurrence rate, staging (7T), grading (G), presence of carcinoma in situ (CIS). Basing on those factors patients with non-muscle invasive bladder cancer were divided into three risk groups. Low risk group - single, diameter < 3 cm, TaG1 tumors, high risk group - multiple, diameter > 3 cm, frequently recurrent, T1G3 tumors with concomitant CIS, intermediate risk group - the rest of tumors not mentioned in prior two groups. When using this division into three risk groups there is no possible to predict the probability of bladder cancer recurrence and progression separately Although prognostic factors may indicate a high risk for recurrence, the risk for progression may still be low and other tumors may have a high risk of both recurrence and progression. In order to separately predict the short-term and long-term risks of both recurrence and progression in individual patients, the EORTC developed a scoring system and risk tables (EORTC nomograms).
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December 2009

Sperm storage and mating in the deep-sea squid Joubin, 1931 (Oegopsida: Octopoteuthidae).

Mar Biol 2010 10;157(2):393-400. Epub 2009 Nov 10.

Earth & Oceanic Sciences Research Institute, Auckland University of Technology, Private Bag 92006, Auckland, 1142 New Zealand.

Spermatangium implantation is reported in the large oceanic squid , based on ten mated females from the stomachs of sperm whales. Implanted spermatangia were located in the mantle, head and neck (on both sides) or above the nuchal cartilage, under the neck collar and were often associated with incisions. These cuts ranged from 30 to 65 mm in length and were probably made by males, using the beak or arm hooks. This is the first time wounds facilitating spermatangium storage have been observed in the internal muscle layers (rather than external, as observed in some other species of squid). The implications of these observations for the mating behavior of the rarely encountered squid are discussed.
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http://dx.doi.org/10.1007/s00227-009-1326-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873075PMC
November 2009

Remarkably low mtDNA control region diversity in an abundant demersal fish.

Mol Phylogenet Evol 2010 Jun 15;55(3):1183-8. Epub 2009 Sep 15.

Evolutionary Genomics Group, Department of Botany and Zoology, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch 7602, South Africa.

Cape hake, Merluccius paradoxus, is a valuable commercially exploited demersal species. Using the 5' mtDNA control region we show that 96% of 1013 fishes sampled over a three-year period share one of two dominant haplotypes; 19 haplotypes were recovered in total, suggesting a genetically homogenous population of fish. Accordingly, haplotype and nucleotide diversities are low (h = 0.53, pi = 0.0014); an asymptotic haplotype accumulation curve suggests that few additional haplotypes exist. Comparing h and pi with other fish species shows that M. paradoxus and other southern African fish species have remarkably low genetic diversity values compared with other global marine fishes. Despite low genetic variability, frequency differences among M. paradoxus haplotypes suggest weakly structured populations between Namibia and South Africa. However, given the remarkably homogeneous mtDNA population genetic structure between fishes sampled along 1800 km, it is clear that faster evolving markers such a microsatellites are also needed before inferences can be made regarding stock identification and management of this species.
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http://dx.doi.org/10.1016/j.ympev.2009.09.018DOI Listing
June 2010

Mitochondrial DNA analyses of the Cape hakes reveal an expanding, panmictic population for Merluccius capensis and population structuring for mature fish in Merluccius paradoxus.

Mol Phylogenet Evol 2007 Feb 11;42(2):517-27. Epub 2006 Aug 11.

Evolutionary Genomics Group, Department of Botany and Zoology, University of Stellenbosch, Private Bag X1, Matieland, Stellenbosch 7602, South Africa.

The Cape hake species, Merluccius capensis and Merluccius paradoxus are the most important resource of the South African and Namibian demersal fishery, but it is unclear whether there is a single population of each shared by both countries. We analysed the population structure and evolutionary history of these two species using the variable 5' region of the mtDNA control region for 311 specimens of M. capensis and 333 specimens of M. paradoxus sampled between Lüderitz (southern Namibia) to south of Cape Point (South Africa). 107 haplotypes for M. capensis and eight haplotypes for M. paradoxus were recovered. AMOVA and pairwise Phi(st) analyses revealed no structure in M. capensis, however significant genetic differentiation between Namibian and South African 'populations' was detected for M. paradoxus. This was only restricted to mature fish older than 3 and 4 years and not for juvenile fish younger than 3 years. Analyses reveal that M. capensis has undergone population expansion (Fu's Fs=-26.65, P<0.001), possibly within the last 4500-23,000 years, whereas M. paradoxus has not. Our study highlights the utility of genetic markers to unravel the evolutionary history of sympatric species, as well as addressing management issues within regions where commercially valuable fish stocks are shared between nations.
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http://dx.doi.org/10.1016/j.ympev.2006.08.004DOI Listing
February 2007

[Experiences in the treatment of benign prostatic hyperplasia with high power potassium-titanyl-phosphate (KTP) laser].

Wiad Lek 2006 ;59(3-4):196-8

Z Kliniki Urologii z Pracownia Litotrypsji Uniwersytetu Medycznego w Lodzi.

Unlabelled: Benign prostatic hyperplasia (BPH) exist in about 70% men after 60 years old. High power KTP laser is a new tool for the treatment of BPH.

Material And Method: 49 men were treated by photoselective vaporisation of the prostate (PVP) from August 2003 to May 2004. Thirty patients with follow-up period longer than 12 weeks (12 to 26 weeks) were analyzed. Preoperative prostate volume ranged from 31 to 136 cc. PSA range, estimated before treatment, was normal in all patients (pts). International Prostate Symptom Score (I-PSS) estimated before treatment was over 24 and Quality of Life (QoL) was over 3 in all pts. The maximum peak urinary flow (Qmax) before treatment ranged from 7.2 to 14.1 ml/s.

Results: One patient was catheterized with Foley catheter at the finish of the procedure. Two pts required catheterization in the first day after treatment. In two pts increasing of body temperature to 38 degrees C was observed in the first day after treatment. Twenty four hours after treatment haematuria required catheterization was observed in one patient. Seven days after treatment in 4 pts massive haematuria was observed (two required hospitalisation). In one patient because of urinary retention 4 weeks after PVP transurethral resection of the prostate was performed. Four weeks after PVP I-PSS decreased from 24 to 20 and after 12 weeks to 15. The Qmax increased and ranged from 11.3 ml/s to 17.1 ml/s 4 weeks after PVP and 12 weeks after PVP ranged from 15.1 to 22.8 ml/s.

Conclusions: Photoselective vaporization of the prostate reduces I-PSS in all patients 12 weeks after procedure. Short follow-up period and a limited number of patients in study group cannot lead to ultimate conclusions. However the results encourage to undertaking further studies on PVP for the treatment of BPH.
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October 2006

[Estrogens in the pathogenesis of coronary artery disease in men].

Pol Arch Med Wewn 2005 Feb;113(2):191-5

Klinika Kardiologii.

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February 2005

[Testosterone and coronary artery disease in men].

Pol Arch Med Wewn 2005 Jan;113(1):92-6

Klinika Kardiologii, Uniwersytet Medyczny w Lodzi.

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January 2005

[Value of the photodynamic method in the diagnosis of superficial bladder tumors].

Wiad Lek 2004 ;57 Suppl 1:192-5

Z Kliniki Urologii i Pracowni Litotrypsji Uniwersytetu Medycznego w Lodzi.

Photodynamic diagnosis (PDD) is one of most precise method of neoplasms recognition. The aim of the study was to estimate the value of 5-amino levulinic acid in diagnosis of superficial bladder tumours. Since 1996 to March 2004, 597 cystoscopies were performed with 5-amino levulinic acid in 151 patients with superficial bladder tumours (102 men and 49 women) aged 19-87 years old. 132 pts were treated by PDD after transurethral resection of bladder tumours (TURB). 749 specimens were taken from mucosa during PDD. Before PDD 50 ml of of 5-amino levulinic acid solution was instilled into bladder. As a source of ultraviolet light (UV) D--Light Storz xenon lamp (405 nm) was used. In 16 pts single PDD was performed in 38 double and in 97 PDD was performed three or more times (3 to 7). All pts were observed 12 months before and after PDD. In 102 from 151 pts in white light cystoscopy (WL) no changes in the bladder mucosa were found. In both kind of light (WL and UV) changes were not observed in 44 pts (29.1%). In 58 pts in WL no changes were observed but in UV places of neoplastic changes in the mucosa were found. In these cases the presence of superficial bladder tumours was confirmed. These changes were pathologically confirmed only in UV in 26 pts. Ta (G1 and G2) changes were confirmed in 52 pts (21 in WL and additionally 31 in UV). T1 changes were confirmed in UV in 26 pts but in WL in 25 pts. In 3 pts T2 changes were confirmed in both UV and WL. Cystoscopy in ultraviolet light (photodynamic diagnosis--PDD) with 5 amino levulinic acid gives more precise diagnosis of superficial bladder tumours then the one in standard white light. Cystoscopy performed to diagnose bladder tumours and early recurrence and new focus of tumours recognition without PDD is less effective. Introduction of superficial bladder tumours photodynamic diagnosis into health care system reduced recurrences and improves results of treatment.
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July 2005