Marco W Fraaije

Fermentation Pilot Plant, Department of Chemical, Biological and Enviromental Engineering, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.

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June 2020

Approaching boiling point stability of an alcohol dehydrogenase through computationally-guided enzyme engineering.

Authors:

Friso S Aalbers Maximilian Jlj Fürst Stefano Rovida Milos Trajkovic J Rubén Gómez Castellanos Sebastian Bartsch Andreas Vogel Andrea Mattevi Marco W Fraaije

Elife 2020 Mar 31;9. Epub 2020 Mar 31.

Molecular Enzymology Group, University of Groningen, Groningen, Netherlands.

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March 2020

Genome Mining of Oxidation Modules in trans-Acyltransferase Polyketide Synthases Reveals a Culturable Source for Lobatamides.

Authors:

Reiko Ueoka Roy A Meoded Alejandro Gran-Scheuch Agneya Bhushan Marco W Fraaije Jörn Piel

Angew Chem Int Ed Engl 2020 05 19;59(20):7761-7765. Epub 2020 Mar 19.

Institute of Microbiology, ETH Zurich, Vladimir-Prelog-Weg 4, 8093, Zurich, Switzerland.

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May 2020

Mechanistic and Crystallographic Studies of Azoreductase AzoA from A01.

Authors:

Elvira Romero Simone Savino Marco W Fraaije Nikola Lončar

ACS Chem Biol 2020 02 29;15(2):504-512. Epub 2020 Jan 29.

GECCO Biotech , Nijenborgh 4 , 9747AG Groningen , The Netherlands.

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February 2020

Publisher Correction: Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs.

Authors:

Callum R Nicoll Gautier Bailleul Filippo Fiorentini María Laura Mascotti Marco W Fraaije Andrea Mattevi

Nat Struct Mol Biol 2020 Feb;27(2):222

Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, Pavia, Italy.

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February 2020

Production of Hydroxy Acids: Selective Double Oxidation of Diols by Flavoprotein Alcohol Oxidase.

Authors:

Caterina Martin Milos Trajkovic Marco W Fraaije

Angew Chem Int Ed Engl 2020 03 4;59(12):4869-4872. Epub 2020 Feb 4.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, Groningen, The Netherlands.

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March 2020

Biocatalytic Enantioselective Oxidation of -Allylic Alcohols with Flavin-Dependent Oxidases.

Authors:

Somayyeh Gandomkar Etta Jost Doris Loidolt Alexander Swoboda Mathias Pickl Wael Elaily Bastian Daniel Marco W Fraaije Peter Macheroux Wolfgang Kroutil

Adv Synth Catal 2019 Nov 10;361(22):5264-5271. Epub 2019 Oct 10.

Institute of Chemistry, NAWI Graz, BioTechMed Graz University of Graz Heinrichstr. 28 8010 Graz Austria.

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November 2019

Creating Flavin Reductase Variants with Thermostable and Solvent-Tolerant Properties by Rational-Design Engineering.

Authors:

Somchart Maenpuen Vinutsada Pongsupasa Wiranee Pensook Piyanuch Anuwan Napatsorn Kraivisitkul Chatchadaporn Pinthong Jittima Phonbuppha Thikumporn Luanloet Hein J Wijma Marco W Fraaije Narin Lawan Pimchai Chaiyen Thanyaporn Wongnate

Chembiochem 2020 05 21;21(10):1481-1491. Epub 2020 Feb 21.

School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), 555 Moo 1 Payupnai, Wangchan, Rayong, 21210, Thailand.

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May 2020

Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs.

Authors:

Callum R Nicoll Gautier Bailleul Filippo Fiorentini María Laura Mascotti Marco W Fraaije Andrea Mattevi

Nat Struct Mol Biol 2020 01 23;27(1):14-24. Epub 2019 Dec 23.

Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, Pavia, Italy.

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January 2020

An overview of microbial indigo-forming enzymes.

Authors:

Andrea N Fabara Marco W Fraaije

Appl Microbiol Biotechnol 2020 Feb 13;104(3):925-933. Epub 2019 Dec 13.

Molecular Enzymology group, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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February 2020

Structure-Based Redesign of a Self-Sufficient Flavin-Containing Monooxygenase towards Indigo Production.

Authors:

Nikola Lončar Hugo L van Beek Marco W Fraaije

Int J Mol Sci 2019 Dec 5;20(24). Epub 2019 Dec 5.

Molecular Enzymology group, University of Groningen, 9747 AG Groningen, The Netherlands.

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December 2019

Stabilization of cyclohexanone monooxygenase by computational and experimental library design.

Authors:

Maximilian J L J Fürst Marjon Boonstra Selle Bandstra Marco W Fraaije

Biotechnol Bioeng 2019 09 24;116(9):2167-2177. Epub 2019 Jun 24.

Molecular Enzymology Group, University of Groningen, Groningen, The Netherlands.

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September 2019

Editorial: , a Source of Biocatalytic Tools.

Authors:

Dirk Tischler Willem J H van Berkel Marco W Fraaije

Front Microbiol 2019 16;10:800. Epub 2019 Apr 16.

Molecular Enzymology, University of Groningen, Groningen, Netherlands.

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April 2019

Characterization of a New DyP-Peroxidase from the Alkaliphilic Cellulomonad, .

Authors:

Mohamed H Habib Henriëtte J Rozeboom Marco W Fraaije

Molecules 2019 Mar 27;24(7). Epub 2019 Mar 27.

Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands.

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March 2019

Beyond active site residues: overall structural dynamics control catalysis in flavin-containing and heme-containing monooxygenases.

Authors:

Maximilian Jlj Fürst Filippo Fiorentini Marco W Fraaije

Curr Opin Struct Biol 2019 12 2;59:29-37. Epub 2019 Mar 2.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands. Electronic address:

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December 2019

Convergent Cascade Catalyzed by Monooxygenase-Alcohol Dehydrogenase Fusion Applied in Organic Media.

Authors:

Lei Huang Friso S Aalbers Wei Tang Robert Röllig Marco W Fraaije Selin Kara

Chembiochem 2019 07 24;20(13):1653-1658. Epub 2019 May 24.

Department of Engineering, Biocatalysis and Bioprocessing Group, Aarhus University, Gustav Wieds Vej 10, 8000, Aarhus, Denmark.

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July 2019

Side-Chain Pruning Has Limited Impact on Substrate Preference in a Promiscuous Enzyme.

Authors:

Maximilian J L J Fürst Elvira Romero J Rúben Gómez Castellanos Marco W Fraaije Andrea Mattevi

ACS Catal 2018 Dec 30;8(12):11648-11656. Epub 2018 Oct 30.

Department of Biology and Biotechnology, University of Pavia, Via Ferrata 1, 27100, Pavia, Italy.

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December 2018

Characterization of a thermostable flavin-containing monooxygenase from Nitrincola lacisaponensis (NiFMO).

Authors:

Nikola Lončar Filippo Fiorentini Gautier Bailleul Simone Savino Elvira Romero Andrea Mattevi Marco W Fraaije

Appl Microbiol Biotechnol 2019 Feb 4;103(4):1755-1764. Epub 2019 Jan 4.

Groningen Enzyme and Cofactor Collection (GECCO), University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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February 2019

Reconstructing the evolutionary history of F-dependent dehydrogenases.

Authors:

M Laura Mascotti Hemant Kumar Quoc-Thai Nguyen Maximiliano Juri Ayub Marco W Fraaije

Sci Rep 2018 12 4;8(1):17571. Epub 2018 Dec 4.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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December 2018

Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.

Authors:

Quoc-Thai Nguyen Elvira Romero Willem P Dijkman Suzan Pantaroto de Vasconcellos Claudia Binda Andrea Mattevi Marco W Fraaije

Biochemistry 2018 10 16;57(43):6209-6218. Epub 2018 Oct 16.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.

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October 2018

Enantio- and regioselective ene-reductions using FH-dependent enzymes.

Authors:

Sam Mathew Milos Trajkovic Hemant Kumar Quoc-Thai Nguyen Marco W Fraaije

Chem Commun (Camb) 2018 Oct;54(79):11208-11211

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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October 2018

Design of Artificial Alcohol Oxidases: Alcohol Dehydrogenase-NADPH Oxidase Fusions for Continuous Oxidations.

Authors:

Friso S Aalbers Marco W Fraaije

Chembiochem 2019 01 4;20(1):51-56. Epub 2018 Oct 4.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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January 2019

Enzyme Fusions in Biocatalysis: Coupling Reactions by Pairing Enzymes.

Authors:

Friso S Aalbers Marco W Fraaije

Chembiochem 2019 01 2;20(1):20-28. Epub 2018 Oct 2.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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January 2019

Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins.

Authors:

Max J L J Fürst Caterina Martin Nikola Lončar Marco W Fraaije

Methods Enzymol 2018 9;608:151-187. Epub 2018 Jun 9.

Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands. Electronic address:

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June 2019

Mining the Genome of : Two New Type I Baeyer-Villiger Monooxygenases From Atacama Desert.

Authors:

Alejandro Gran-Scheuch Milos Trajkovic Loreto Parra Marco W Fraaije

Front Microbiol 2018 18;9:1609. Epub 2018 Jul 18.

Molecular Enzymology Group, University of Groningen, Groningen, Netherlands.

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July 2018

Overriding Traditional Electronic Effects in Biocatalytic Baeyer-Villiger Reactions by Directed Evolution.

Authors:

Guangyue Li Marc Garcia-Borràs Maximilian J L J Fürst Adriana Ilie Marco W Fraaije K N Houk Manfred T Reetz

J Am Chem Soc 2018 08 13;140(33):10464-10472. Epub 2018 Aug 13.

Max-Planck-Institut für Kohlenforschung , Kaiser-Wilhelm-Platz 1 , 45470 Mülheim , Germany.

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August 2018

The Biocatalytic Synthesis of Syringaresinol from 2,6-Dimethoxy-4-allylphenol in One-Pot Using a Tailored Oxidase/Peroxidase System.

Authors:

Mohamed Habib Milos Trajkovic Marco W Fraaije

ACS Catal 2018 Jun 14;8(6):5549-5552. Epub 2018 May 14.

Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen 9747 AG, The Netherlands.

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June 2018

Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design.

Authors:

Caterina Martin Amaury Ovalle Maqueo Hein J Wijma Marco W Fraaije

Biotechnol Biofuels 2018 1;11:56. Epub 2018 Mar 1.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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March 2018

Kinetic Resolution of sec-Thiols by Enantioselective Oxidation with Rationally Engineered 5-(Hydroxymethyl)furfural Oxidase.

Authors:

Mathias Pickl Alexander Swoboda Elvira Romero Christoph K Winkler Claudia Binda Andrea Mattevi Kurt Faber Marco W Fraaije

Angew Chem Int Ed Engl 2018 03 15;57(11):2864-2868. Epub 2018 Feb 15.

Department of Biotechnology, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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March 2018

Same Substrate, Many Reactions: Oxygen Activation in Flavoenzymes.

Authors:

Elvira Romero J Rubén Gómez Castellanos Giovanni Gadda Marco W Fraaije Andrea Mattevi

Chem Rev 2018 02 11;118(4):1742-1769. Epub 2018 Jan 11.

Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia , Via Ferrata 9, 27100 Pavia, Italy.

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February 2018

Characterization of Two VAO-Type Flavoprotein Oxidases from Myceliophthora thermophila.

Authors:

Alessandro R Ferrari Henriëtte J Rozeboom Aniek S C Vugts Martijn J Koetsier Robert Floor Marco W Fraaije

Molecules 2018 Jan 5;23(1). Epub 2018 Jan 5.

Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747AG Groningen, The Netherlands.

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January 2018

Molecular Basis for Converting (2S)-Methylsuccinyl-CoA Dehydrogenase into an Oxidase.

Authors:

Simon Burgener Thomas Schwander Elvira Romero Marco W Fraaije Tobias J Erb

Molecules 2017 Dec 28;23(1). Epub 2017 Dec 28.

Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043 Marburg, Germany.

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December 2017

Rational Engineering of a Flavoprotein Oxidase for Improved Direct Oxidation of Alcohols to Carboxylic Acids.

Authors:

Mathias Pickl Christoph K Winkler Silvia M Glueck Marco W Fraaije Kurt Faber

Molecules 2017 Dec 12;22(12). Epub 2017 Dec 12.

Department of Chemistry, University of Graz, Heinrichstrasse 28, A-8010 Graz, Austria.

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December 2017

Exploring PTDH-P450BM3 Variants for the Synthesis of Drug Metabolites.

Authors:

Nina Beyer Justyna K Kulig Marco W Fraaije Martin A Hayes Dick B Janssen

Chembiochem 2018 02 17;19(4):326-337. Epub 2018 Jan 17.

Biotransformation and Biocatalysis, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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February 2018

Manipulating the stereoselectivity of the thermostable Baeyer-Villiger monooxygenase TmCHMO by directed evolution.

Authors:

Guangyue Li Maximilian J L J Fürst Hamid Reza Mansouri Anna K Ressmann Adriana Ilie Florian Rudroff Marko D Mihovilovic Marco W Fraaije Manfred T Reetz

Org Biomol Chem 2017 Nov;15(46):9824-9829

Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470, Mülheim an der Ruhr, Germany.

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November 2017

Exploring the Substrate Scope of Baeyer-Villiger Monooxygenases with Branched Lactones as Entry towards Polyesters.

Authors:

Marie A F Delgove Maximilian J L J Fürst Marco W Fraaije Katrien V Bernaerts Stefaan M A De Wildeman

Chembiochem 2018 02 29;19(4):354-360. Epub 2017 Nov 29.

Maastricht University, Aachen-Maastricht Institute for Biobased Materials (AMIBM), Brightlands Chemelot Campus, Urmonderbaan 22, 6167 RD, Geleen, The Netherlands.

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February 2018

Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase-cyclohexanone monooxygenase fusions.

Authors:

Friso S Aalbers Marco W Fraaije

Appl Microbiol Biotechnol 2017 Oct 15;101(20):7557-7565. Epub 2017 Sep 15.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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October 2017

Creating Oxidase-Peroxidase Fusion Enzymes as a Toolbox for Cascade Reactions.

Authors:

Dana I Colpa Nikola Lončar Mareike Schmidt Marco W Fraaije

Chembiochem 2017 11 11;18(22):2226-2230. Epub 2017 Oct 11.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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November 2017

Baeyer-Villiger Monooxygenase FMO5 as Entry Point in Drug Metabolism.

Authors:

Filippo Fiorentini Elvira Romero Marco W Fraaije Kurt Faber Mélanie Hall Andrea Mattevi

ACS Chem Biol 2017 09 21;12(9):2379-2387. Epub 2017 Aug 21.

Austrian Centre of Industrial Biotechnology, c/o Department of Chemistry, University of Graz , Heinrichstrasse 28, 8010 Graz, Austria.

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September 2017

Two tyrosine residues, Tyr-108 and Tyr-503, are responsible for the deprotonation of phenolic substrates in vanillyl-alcohol oxidase.

Authors:

Tom A Ewing Quoc-Thai Nguyen Robert C Allan Gudrun Gygli Elvira Romero Claudia Binda Marco W Fraaije Andrea Mattevi Willem J H van Berkel

J Biol Chem 2017 09 17;292(35):14668-14679. Epub 2017 Jul 17.

From the Laboratory of Biochemistry, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands,

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September 2017

The VAO/PCMH flavoprotein family.

Authors:

Tom A Ewing Marco W Fraaije Andrea Mattevi Willem J H van Berkel

Arch Biochem Biophys 2017 10 30;632:104-117. Epub 2017 Jun 30.

Laboratory of Biochemistry, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands. Electronic address:

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October 2017

Crystal structures and atomic model of NADPH oxidase.

Authors:

Francesca Magnani Simone Nenci Elisa Millana Fananas Marta Ceccon Elvira Romero Marco W Fraaije Andrea Mattevi

Proc Natl Acad Sci U S A 2017 06 12;114(26):6764-6769. Epub 2017 Jun 12.

Department of Biology and Biotechnology "L. Spallanzani," University of Pavia, 27100 Pavia, Italy;

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June 2017

Isolation and characterization of a thermostable F:NADPH oxidoreductase from .

Authors:

Hemant Kumar Quoc-Thai Nguyen Claudia Binda Andrea Mattevi Marco W Fraaije

J Biol Chem 2017 06 14;292(24):10123-10130. Epub 2017 Apr 14.

From the Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands,

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June 2017

Polycyclic Ketone Monooxygenase from the Thermophilic Fungus Thermothelomyces thermophila: A Structurally Distinct Biocatalyst for Bulky Substrates.

Authors:

Maximilian J L J Fürst Simone Savino Hanna M Dudek J Rúben Gómez Castellanos Cora Gutiérrez de Souza Stefano Rovida Marco W Fraaije Andrea Mattevi

J Am Chem Soc 2017 01 3;139(2):627-630. Epub 2017 Jan 3.

Department of Biology and Biotechnology, University of Pavia , Via Ferrata 1, 27100, Pavia, Italy.

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January 2017

Discovery and characterization of an F-dependent glucose-6-phosphate dehydrogenase (Rh-FGD1) from Rhodococcus jostii RHA1.

Authors:

Quoc-Thai Nguyen Gianluca Trinco Claudia Binda Andrea Mattevi Marco W Fraaije

Appl Microbiol Biotechnol 2017 Apr 13;101(7):2831-2842. Epub 2016 Dec 13.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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April 2017

Engineering Cyclohexanone Monooxygenase for the Production of Methyl Propanoate.

Authors:

Hugo L van Beek Elvira Romero Marco W Fraaije

ACS Chem Biol 2017 01 20;12(1):291-299. Epub 2016 Dec 20.

Molecular Enzymology Group, University of Groningen , Nijenborgh 4, 9747AG Groningen, The Netherlands.

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January 2017

P450 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations.

Authors:

Nina Beyer Justyna K Kulig Anette Bartsch Martin A Hayes Dick B Janssen Marco W Fraaije

Appl Microbiol Biotechnol 2017 Mar 29;101(6):2319-2331. Epub 2016 Nov 29.

Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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March 2017

Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase.

Authors:

Elvira Romero J Rubén Gómez Castellanos Andrea Mattevi Marco W Fraaije

Angew Chem Int Ed Engl 2016 12 22;55(51):15852-15855. Epub 2016 Nov 22.

Department of Biotechnology, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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December 2016

Discovery of a Xylooligosaccharide Oxidase from Myceliophthora thermophila C1.

Authors:

Alessandro R Ferrari Henriëtte J Rozeboom Justyna M Dobruchowska Sander S van Leeuwen Aniek S C Vugts Martijn J Koetsier Jaap Visser Marco W Fraaije

J Biol Chem 2016 Nov 14;291(45):23709-23718. Epub 2016 Sep 14.

From the Molecular Enzymology Group and

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November 2016

Bacterial enzymes involved in lignin degradation.

Authors:

Gonzalo de Gonzalo Dana I Colpa Mohamed H M Habib Marco W Fraaije

J Biotechnol 2016 Oct 17;236:110-9. Epub 2016 Aug 17.

Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Electronic address:

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October 2016

Biocatalytic Properties and Structural Analysis of Eugenol Oxidase from Rhodococcus jostii RHA1: A Versatile Oxidative Biocatalyst.

Authors:

Quoc-Thai Nguyen Gonzalo de Gonzalo Claudia Binda Ana Rioz-Martínez Andrea Mattevi Marco W Fraaije

Chembiochem 2016 07 7;17(14):1359-66. Epub 2016 Jun 7.

Molecular Enzymology, Groningen Biomolecular Sciences and, Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, NL.

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July 2016

Characterization of a chitinase from the cellulolytic actinomycete Thermobifida fusca.

Authors:

Yasser Gaber Sophanit Mekasha Gustav Vaaje-Kolstad Vincent G H Eijsink Marco W Fraaije

Biochim Biophys Acta 2016 09 22;1864(9):1253-1259. Epub 2016 Apr 22.

University of Groningen, Molecular Enzymology Group, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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September 2016

Microbial Flavoprotein Monooxygenases as Mimics of Mammalian Flavin-Containing Monooxygenases for the Enantioselective Preparation of Drug Metabolites.

Authors:

Turan Gul Marzena Krzek Hjalmar P Permentier Marco W Fraaije Rainer Bischoff

Drug Metab Dispos 2016 08 16;44(8):1270-6. Epub 2016 Mar 16.

Analytical Biochemistry, Groningen Research Institute of Pharmacy (T.G., H.P.P., R.B.), Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute (M.K., M.W.F.), and Interfaculty Mass Spectrometry Center (H.P.P.), University of Groningen, Groningen, the Netherlands.

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August 2016

Covalent immobilization of a flavoprotein monooxygenase via its flavin cofactor.

Authors:

Marzena Krzek Hugo L van Beek Hjalmar P Permentier Rainer Bischoff Marco W Fraaije

Enzyme Microb Technol 2016 Jan 30;82:138-143. Epub 2015 Sep 30.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands. Electronic address:

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January 2016

Erratum: Cloning, overexpression and biocatalytic exploration of a novel Baeyer-Villiger monooxygenase from Aspergillus fumigatus Af293.

Authors:

Maria Laura Mascotti Maximiliano Juri Ayub Hanna Dudek Marcela Kurina Sanz Marco W Fraaije

AMB Express 2014 Dec 20;4(1):62. Epub 2014 Sep 20.

INTEQUI-CONICET, Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, CP 5700, San Luis, Argentina,

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December 2014

Lyophilization conditions for the storage of monooxygenases.

Authors:

Hugo L van Beek Nina Beyer Dick B Janssen Marco W Fraaije

J Biotechnol 2015 Jun 26;203:41-4. Epub 2015 Mar 26.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Electronic address:

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June 2015

Catalases as biocatalysts in technical applications: current state and perspectives.

Authors:

Nikola Lončar Marco W Fraaije

Appl Microbiol Biotechnol 2015 Apr 12;99(8):3351-7. Epub 2015 Mar 12.

Faculty of Chemistry, University of Belgrade, Studentskitrg 12-16, Belgrade, Serbia.

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April 2015

Expanding the substrate scope of chitooligosaccharide oxidase from Fusarium graminearum by structure-inspired mutagenesis.

Authors:

Alessandro R Ferrari Misun Lee Marco W Fraaije

Biotechnol Bioeng 2015 Jun 2;112(6):1074-80. Epub 2015 Mar 2.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

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June 2015

Finding the switch: turning a baeyer-villiger monooxygenase into a NADPH oxidase.

Authors:

Patrícia B Brondani Hanna M Dudek Christian Martinoli Andrea Mattevi Marco W Fraaije

J Am Chem Soc 2014 Dec 1;136(49):16966-9. Epub 2014 Dec 1.

Molecular Enzymology Group, University of Groningen , Nijenborgh 4, 9747AG Groningen, The Netherlands.

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December 2014

The oxidation of thiols by flavoprotein oxidases: a biocatalytic route to reactive thiocarbonyls.

Authors:

Tom A Ewing Willem P Dijkman Jacques M Vervoort Marco W Fraaije Willem J H van Berkel

Angew Chem Int Ed Engl 2014 Nov 5;53(48):13206-9. Epub 2014 Oct 5.

Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3 (The Netherlands) http://www.bic.wur.nl.

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November 2014

Insights in the kinetic mechanism of the eukaryotic Baeyer-Villiger monooxygenase BVMOAf1 from Aspergillus fumigatus Af293.

Authors:

María Laura Mascotti Marcela Kurina-Sanz Maximiliano Juri Ayub Marco W Fraaije

Biochimie 2014 Dec 16;107 Pt B:270-6. Epub 2014 Sep 16.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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December 2014

Not so monofunctional--a case of thermostable Thermobifida fusca catalase with peroxidase activity.

Authors:

Nikola Lončar Marco W Fraaije

Appl Microbiol Biotechnol 2015 Mar 17;99(5):2225-32. Epub 2014 Sep 17.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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March 2015

Synthesis of methyl propanoate by Baeyer-Villiger monooxygenases.

Authors:

Hugo L van Beek Remko T Winter Graham R Eastham Marco W Fraaije

Chem Commun (Camb) 2014 Nov;50(86):13034-6

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Netherlands.

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November 2014

A rapid quantitative activity assay shows that the Vibrio cholerae colonization factor GbpA is an active lytic polysaccharide monooxygenase.

Authors:

Jennifer S M Loose Zarah Forsberg Marco W Fraaije Vincent G H Eijsink Gustav Vaaje-Kolstad

FEBS Lett 2014 Sep 7;588(18):3435-40. Epub 2014 Aug 7.

Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O. Box 5003, N-1432 Aas, Norway. Electronic address:

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September 2014

Kinetic mechanism of putrescine oxidase from Rhodococcus erythropolis.

Authors:

Malgorzata M Kopacz Dominic P H M Heuts Marco W Fraaije

FEBS J 2014 Oct 15;281(19):4384-93. Epub 2014 Aug 15.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.

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October 2014

Turning a monocovalent flavoprotein into a bicovalent flavoprotein by structure-inspired mutagenesis.

Authors:

Malgorzata M Kopacz Marco W Fraaije

Bioorg Med Chem 2014 Oct 4;22(20):5621-7. Epub 2014 Jun 4.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Electronic address:

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October 2014

The taming of oxygen: biocatalytic oxyfunctionalisations.

Authors:

Dirk Holtmann Marco W Fraaije Isabel W C E Arends Diederik J Opperman Frank Hollmann

Chem Commun (Camb) 2014 Nov;50(87):13180-200

DECHEMA Research Institute, Theodor-Heuss-Allee 25, 60486 Frankfurt am Main, Germany.

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November 2014

Enzyme-catalyzed oxidation of 5-hydroxymethylfurfural to furan-2,5-dicarboxylic acid.

Authors:

Willem P Dijkman Daphne E Groothuis Marco W Fraaije

Angew Chem Int Ed Engl 2014 Jun 6;53(25):6515-8. Epub 2014 May 6.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen (The Netherlands) http://www.rug.nl/staff/m.w.fraaije/research.

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June 2014

Stabilization of cyclohexanone monooxygenase by a computationally designed disulfide bond spanning only one residue.

Authors:

Hugo L van Beek Hein J Wijma Lucie Fromont Dick B Janssen Marco W Fraaije

FEBS Open Bio 2014 3;4:168-74. Epub 2014 Feb 3.

Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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March 2014

A fast, sensitive and easy colorimetric assay for chitinase and cellulase activity detection.

Authors:

Alessandro R Ferrari Yasser Gaber Marco W Fraaije

Biotechnol Biofuels 2014 Mar 10;7(1):37. Epub 2014 Mar 10.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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March 2014

Beyond the Protein Matrix: Probing Cofactor Variants in a Baeyer-Villiger Oxygenation Reaction.

Authors:

Christian Martinoli Hanna M Dudek Roberto Orru Dale E Edmondson Marco W Fraaije Andrea Mattevi

ACS Catal 2013 ;3(12):3058-3062

Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.

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January 2013

Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688.

Authors:

Willem P Dijkman Marco W Fraaije

Appl Environ Microbiol 2014 Feb 22;80(3):1082-90. Epub 2013 Nov 22.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

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February 2014

Cascade reactions in multicompartmentalized polymersomes.

Authors:

Ruud J R W Peters Maïté Marguet Sébastien Marais Marco W Fraaije Jan C M van Hest Sébastien Lecommandoux

Angew Chem Int Ed Engl 2014 Jan 19;53(1):146-50. Epub 2013 Nov 19.

Radboud University Nijmegen, Institute for Molecules and Materials, Bio-organic Chemistry, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands).

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January 2014

Extending the substrate scope of a Baeyer-Villiger monooxygenase by multiple-site mutagenesis.

Authors:

Hanna M Dudek Michael J Fink Amol V Shivange Alexander Dennig Marko D Mihovilovic Ulrich Schwaneberg Marco W Fraaije

Appl Microbiol Biotechnol 2014 May 19;98(9):4009-20. Epub 2013 Nov 19.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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May 2014

DyP-type peroxidases: a promising and versatile class of enzymes.

Authors:

Dana I Colpa Marco W Fraaije Edwin van Bloois

J Ind Microbiol Biotechnol 2014 Jan 9;41(1):1-7. Epub 2013 Nov 9.

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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January 2014

Cloning, overexpression and biocatalytic exploration of a novel Baeyer-Villiger monooxygenase from Aspergillus fumigatus Af293.

Authors:

Maria Laura Mascotti Maximiliano Juri Ayub Hanna Dudek Marcela Kurina Sanz Marco W Fraaije

AMB Express 2013 06 14;3(1):33. Epub 2013 Jun 14.

INTEQUI-CONICET, Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, CP 5700, Argentina.

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June 2013

Special issue: Catalytic mechanisms by biological systems--introduction.

Authors:

Marco W Fraaije Nigel S Scrutton

FEBS J 2013 Jul 3;280(13):2947. Epub 2013 Jun 3.

Molecular Enzymology Group, University of Groningen, Groningen, The Netherlands.

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July 2013

Flavoprotein oxidases: classification and applications.

Authors:

Willem P Dijkman Gonzalo de Gonzalo Andrea Mattevi Marco W Fraaije

Appl Microbiol Biotechnol 2013 Jun 3;97(12):5177-88. Epub 2013 May 3.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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June 2013

A generic, whole-cell-based screening method for Baeyer-Villiger monooxygenases.

Authors:

Hanna M Dudek Petra Popken Edwin van Bloois Wouter A Duetz Marco W Fraaije

J Biomol Screen 2013 Jul 27;18(6):678-87. Epub 2013 Mar 27.

Molecular Enzymology Group, University of Groningen, Groningen, The Netherlands.

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July 2013

Precursor of ether phospholipids is synthesized by a flavoenzyme through covalent catalysis.

Authors:

Simone Nenci Valentina Piano Sara Rosati Alessandro Aliverti Vittorio Pandini Marco W Fraaije Albert J R Heck Dale E Edmondson Andrea Mattevi

Proc Natl Acad Sci U S A 2012 Nov 29;109(46):18791-6. Epub 2012 Oct 29.

Department of Biology and Biotechnology, University of Pavia, 27100 Pavia, Italy.

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November 2012

The enigmatic reaction of flavins with oxygen.

Authors:

Pimchai Chaiyen Marco W Fraaije Andrea Mattevi

Trends Biochem Sci 2012 Sep 20;37(9):373-80. Epub 2012 Jul 20.

Department of Biochemistry and Center of Excellence in Protein Structure and Function, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand.

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September 2012

A stepwise approach for the reproducible optimization of PAMO expression in Escherichia coli for whole-cell biocatalysis.

Authors:

Edwin van Bloois Hanna M Dudek Wouter A Duetz Marco W Fraaije

BMC Biotechnol 2012 Jun 21;12:31. Epub 2012 Jun 21.

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.

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June 2012

Exploring the structural basis of substrate preferences in Baeyer-Villiger monooxygenases: insight from steroid monooxygenase.

Authors:

Stefano Franceschini Hugo L van Beek Alessandra Pennetta Christian Martinoli Marco W Fraaije Andrea Mattevi

J Biol Chem 2012 Jun 17;287(27):22626-34. Epub 2012 May 17.

Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.

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June 2012

Blending Baeyer-Villiger monooxygenases: using a robust BVMO as a scaffold for creating chimeric enzymes with novel catalytic properties.

Authors:

Hugo L van Beek Gonzalo de Gonzalo Marco W Fraaije

Chem Commun (Camb) 2012 Apr 27;48(27):3288-90. Epub 2012 Jan 27.

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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April 2012

Hot or not? Discovery and characterization of a thermostable alditol oxidase from Acidothermus cellulolyticus 11B.

Authors:

Remko T Winter Dominic P H M Heuts Egon M A Rijpkema Edwin van Bloois Hein J Wijma Marco W Fraaije

Appl Microbiol Biotechnol 2012 Jul 11;95(2):389-403. Epub 2012 Jan 11.

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, The Netherlands.

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July 2012

Functionalization of oxidases with peroxidase activity creates oxiperoxidases: a new breed of hybrid enzyme capable of cascade chemistry.

Authors:

Remko T Winter Tomas E van den Berg Dana I Colpa Edwin van Bloois Marco W Fraaije

Chembiochem 2012 Jan 23;13(2):252-8. Epub 2011 Dec 23.

Laboratory of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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January 2012

Risk assessment studies on succinate dehydrogenase inhibitors, the new weapons in the battle to control Septoria leaf blotch in wheat.

Authors:

Bart A Fraaije Carlos Bayon Sarah Atkins Hans J Cools John A Lucas Marco W Fraaije

Mol Plant Pathol 2012 Apr 20;13(3):263-75. Epub 2011 Sep 20.

Rothamsted Research, Plant Pathology and Microbiology Department, Harpenden, Hertfordshire AL5 2JQ, UK.

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April 2012

Turning a riboflavin-binding protein into a self-sufficient monooxygenase by cofactor redesign.

Authors:

Gonzalo de Gonzalo Christian Smit Jianfeng Jin Adriaan J Minnaard Marco W Fraaije

Chem Commun (Camb) 2011 Oct 8;47(39):11050-2. Epub 2011 Sep 8.

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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October 2011

Dynamic kinetic resolution of α-substituted β-ketoesters catalyzed by Baeyer-Villiger monooxygenases: access to enantiopure α-hydroxy esters.

Authors:

Ana Rioz-Martínez Aníbal Cuetos Cristina Rodríguez Gonzalo de Gonzalo Iván Lavandera Marco W Fraaije Vicente Gotor

Angew Chem Int Ed Engl 2011 Aug 15;50(36):8387-90. Epub 2011 Jul 15.

Dpto de Química Orgánica e Inorgánica, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo c/Julián Clavería 8, 33006 Oviedo, Spain.

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August 2011

Mapping the substrate binding site of phenylacetone monooxygenase from Thermobifida fusca by mutational analysis.

Authors:

Hanna M Dudek Gonzalo de Gonzalo Daniel E Torres Pazmiño Piotr Stepniak Lucjan S Wyrwicz Leszek Rychlewski Marco W Fraaije

Appl Environ Microbiol 2011 Aug 1;77(16):5730-8. Epub 2011 Jul 1.

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

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August 2011

Publications by authors named "Marco W Fraaije"

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