Publications by authors named "Marco W Fraaije"

100Publications

Production of indigo through the use of a dual-function substrate and a bifunctional fusion enzyme.

Enzyme Microb Technol 2020 Dec 14;142:109692. Epub 2020 Oct 14.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands. Electronic address:

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December 2020

The vast repertoire of carbohydrate oxidases: An overview.

Biotechnol Adv 2020 Sep 19:107634. Epub 2020 Sep 19.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG Groningen, the Netherlands. Electronic address:

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September 2020

Vanillyl alcohol oxidase.

Enzymes 2020 18;47:87-116. Epub 2020 Jul 18.

Laboratory of Food Chemistry, Wageningen University & Research, Wageningen, The Netherlands. Electronic address:

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December 2020

The multipurpose family of flavoprotein oxidases.

Enzymes 2020 18;47:63-86. Epub 2020 Jul 18.

Department of Biology and Biotechnology, University of Pavia, Pavia, Italy.

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December 2020

Facile Stereoselective Reduction of Prochiral Ketones by using an F -dependent Alcohol Dehydrogenase.

Chembiochem 2021 Jan 23;22(1):156-159. Epub 2020 Oct 23.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, Groningen, The Netherlands.

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January 2021

Substrate binding tunes the reactivity of hispidin 3-hydroxylase, a flavoprotein monooxygenase involved in fungal bioluminescence.

J Biol Chem 2020 Nov 11;295(47):16013-16022. Epub 2020 Sep 11.

Molecular Enzymology Group, University of Groningen, Groningen, The Netherlands

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November 2020

Structure of a robust bacterial protein cage and its application as a versatile biocatalytic platform through enzyme encapsulation.

Biochem Biophys Res Commun 2020 Aug 15;529(3):548-553. Epub 2020 Jul 15.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG, Groningen, the Netherlands. Electronic address:

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August 2020

Analysis of the structure and substrate scope of chitooligosaccharide oxidase reveals high affinity for C2-modified glucosamines.

FEBS Lett 2020 Sep 18;594(17):2819-2828. Epub 2020 Jun 18.

Molecular Enzymology Group, University of Groningen, The Netherlands.

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September 2020

High-level production of industrially relevant oxidases by a two-stage fed-batch approach: overcoming catabolite repression in arabinose-inducible Escherichia coli systems.

Appl Microbiol Biotechnol 2020 Jun 22;104(12):5337-5345. Epub 2020 Apr 22.

Fermentation Pilot Plant, Department of Chemical, Biological and Enviromental Engineering, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.

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June 2020

Genome Mining of Oxidation Modules in trans-Acyltransferase Polyketide Synthases Reveals a Culturable Source for Lobatamides.

Angew Chem Int Ed Engl 2020 05 19;59(20):7761-7765. Epub 2020 Mar 19.

Institute of Microbiology, ETH Zurich, Vladimir-Prelog-Weg 4, 8093, Zurich, Switzerland.

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May 2020

Mechanistic and Crystallographic Studies of Azoreductase AzoA from A01.

ACS Chem Biol 2020 02 29;15(2):504-512. Epub 2020 Jan 29.

GECCO Biotech , Nijenborgh 4 , 9747AG Groningen , The Netherlands.

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February 2020

Publisher Correction: Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs.

Nat Struct Mol Biol 2020 Feb;27(2):222

Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, Pavia, Italy.

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February 2020

Production of Hydroxy Acids: Selective Double Oxidation of Diols by Flavoprotein Alcohol Oxidase.

Angew Chem Int Ed Engl 2020 03 4;59(12):4869-4872. Epub 2020 Feb 4.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, Groningen, The Netherlands.

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March 2020

Biocatalytic Enantioselective Oxidation of -Allylic Alcohols with Flavin-Dependent Oxidases.

Adv Synth Catal 2019 Nov 10;361(22):5264-5271. Epub 2019 Oct 10.

Institute of Chemistry, NAWI Graz, BioTechMed Graz University of Graz Heinrichstr. 28 8010 Graz Austria.

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November 2019

Creating Flavin Reductase Variants with Thermostable and Solvent-Tolerant Properties by Rational-Design Engineering.

Chembiochem 2020 05 21;21(10):1481-1491. Epub 2020 Feb 21.

School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), 555 Moo 1 Payupnai, Wangchan, Rayong, 21210, Thailand.

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May 2020

Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs.

Nat Struct Mol Biol 2020 01 23;27(1):14-24. Epub 2019 Dec 23.

Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, Pavia, Italy.

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January 2020

An overview of microbial indigo-forming enzymes.

Appl Microbiol Biotechnol 2020 Feb 13;104(3):925-933. Epub 2019 Dec 13.

Molecular Enzymology group, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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February 2020

Structure-Based Redesign of a Self-Sufficient Flavin-Containing Monooxygenase towards Indigo Production.

Int J Mol Sci 2019 Dec 5;20(24). Epub 2019 Dec 5.

Molecular Enzymology group, University of Groningen, 9747 AG Groningen, The Netherlands.

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December 2019

Stabilization of cyclohexanone monooxygenase by computational and experimental library design.

Biotechnol Bioeng 2019 09 24;116(9):2167-2177. Epub 2019 Jun 24.

Molecular Enzymology Group, University of Groningen, Groningen, The Netherlands.

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September 2019

Editorial: , a Source of Biocatalytic Tools.

Front Microbiol 2019 16;10:800. Epub 2019 Apr 16.

Molecular Enzymology, University of Groningen, Groningen, Netherlands.

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April 2019

Characterization of a New DyP-Peroxidase from the Alkaliphilic Cellulomonad, .

Molecules 2019 Mar 27;24(7). Epub 2019 Mar 27.

Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands.

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March 2019

Beyond active site residues: overall structural dynamics control catalysis in flavin-containing and heme-containing monooxygenases.

Curr Opin Struct Biol 2019 12 2;59:29-37. Epub 2019 Mar 2.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands. Electronic address:

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December 2019

Convergent Cascade Catalyzed by Monooxygenase-Alcohol Dehydrogenase Fusion Applied in Organic Media.

Chembiochem 2019 07 24;20(13):1653-1658. Epub 2019 May 24.

Department of Engineering, Biocatalysis and Bioprocessing Group, Aarhus University, Gustav Wieds Vej 10, 8000, Aarhus, Denmark.

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July 2019

Side-Chain Pruning Has Limited Impact on Substrate Preference in a Promiscuous Enzyme.

ACS Catal 2018 Dec 30;8(12):11648-11656. Epub 2018 Oct 30.

Department of Biology and Biotechnology, University of Pavia, Via Ferrata 1, 27100, Pavia, Italy.

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December 2018

Characterization of a thermostable flavin-containing monooxygenase from Nitrincola lacisaponensis (NiFMO).

Appl Microbiol Biotechnol 2019 Feb 4;103(4):1755-1764. Epub 2019 Jan 4.

Groningen Enzyme and Cofactor Collection (GECCO), University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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February 2019

Reconstructing the evolutionary history of F-dependent dehydrogenases.

Sci Rep 2018 12 4;8(1):17571. Epub 2018 Dec 4.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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December 2018

Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.

Biochemistry 2018 10 16;57(43):6209-6218. Epub 2018 Oct 16.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.

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October 2018

Enantio- and regioselective ene-reductions using FH-dependent enzymes.

Chem Commun (Camb) 2018 Oct;54(79):11208-11211

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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October 2018

Design of Artificial Alcohol Oxidases: Alcohol Dehydrogenase-NADPH Oxidase Fusions for Continuous Oxidations.

Chembiochem 2019 01 4;20(1):51-56. Epub 2018 Oct 4.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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January 2019

Enzyme Fusions in Biocatalysis: Coupling Reactions by Pairing Enzymes.

Chembiochem 2019 01 2;20(1):20-28. Epub 2018 Oct 2.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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January 2019

Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins.

Methods Enzymol 2018 9;608:151-187. Epub 2018 Jun 9.

Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands. Electronic address:

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June 2019

Mining the Genome of : Two New Type I Baeyer-Villiger Monooxygenases From Atacama Desert.

Front Microbiol 2018 18;9:1609. Epub 2018 Jul 18.

Molecular Enzymology Group, University of Groningen, Groningen, Netherlands.

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July 2018

Overriding Traditional Electronic Effects in Biocatalytic Baeyer-Villiger Reactions by Directed Evolution.

J Am Chem Soc 2018 08 13;140(33):10464-10472. Epub 2018 Aug 13.

Max-Planck-Institut für Kohlenforschung , Kaiser-Wilhelm-Platz 1 , 45470 Mülheim , Germany.

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August 2018

The Biocatalytic Synthesis of Syringaresinol from 2,6-Dimethoxy-4-allylphenol in One-Pot Using a Tailored Oxidase/Peroxidase System.

ACS Catal 2018 Jun 14;8(6):5549-5552. Epub 2018 May 14.

Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen 9747 AG, The Netherlands.

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June 2018

Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design.

Biotechnol Biofuels 2018 1;11:56. Epub 2018 Mar 1.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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March 2018

Kinetic Resolution of sec-Thiols by Enantioselective Oxidation with Rationally Engineered 5-(Hydroxymethyl)furfural Oxidase.

Angew Chem Int Ed Engl 2018 03 15;57(11):2864-2868. Epub 2018 Feb 15.

Department of Biotechnology, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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March 2018

Same Substrate, Many Reactions: Oxygen Activation in Flavoenzymes.

Chem Rev 2018 02 11;118(4):1742-1769. Epub 2018 Jan 11.

Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia , Via Ferrata 9, 27100 Pavia, Italy.

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February 2018

Characterization of Two VAO-Type Flavoprotein Oxidases from Myceliophthora thermophila.

Molecules 2018 Jan 5;23(1). Epub 2018 Jan 5.

Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747AG Groningen, The Netherlands.

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January 2018

Molecular Basis for Converting (2S)-Methylsuccinyl-CoA Dehydrogenase into an Oxidase.

Molecules 2017 Dec 28;23(1). Epub 2017 Dec 28.

Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043 Marburg, Germany.

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December 2017

Rational Engineering of a Flavoprotein Oxidase for Improved Direct Oxidation of Alcohols to Carboxylic Acids.

Molecules 2017 Dec 12;22(12). Epub 2017 Dec 12.

Department of Chemistry, University of Graz, Heinrichstrasse 28, A-8010 Graz, Austria.

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December 2017

Exploring PTDH-P450BM3 Variants for the Synthesis of Drug Metabolites.

Chembiochem 2018 02 17;19(4):326-337. Epub 2018 Jan 17.

Biotransformation and Biocatalysis, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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February 2018

Exploring the Substrate Scope of Baeyer-Villiger Monooxygenases with Branched Lactones as Entry towards Polyesters.

Chembiochem 2018 02 29;19(4):354-360. Epub 2017 Nov 29.

Maastricht University, Aachen-Maastricht Institute for Biobased Materials (AMIBM), Brightlands Chemelot Campus, Urmonderbaan 22, 6167 RD, Geleen, The Netherlands.

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February 2018

Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase-cyclohexanone monooxygenase fusions.

Appl Microbiol Biotechnol 2017 Oct 15;101(20):7557-7565. Epub 2017 Sep 15.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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October 2017

Creating Oxidase-Peroxidase Fusion Enzymes as a Toolbox for Cascade Reactions.

Chembiochem 2017 11 11;18(22):2226-2230. Epub 2017 Oct 11.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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November 2017

Baeyer-Villiger Monooxygenase FMO5 as Entry Point in Drug Metabolism.

ACS Chem Biol 2017 09 21;12(9):2379-2387. Epub 2017 Aug 21.

Austrian Centre of Industrial Biotechnology, c/o Department of Chemistry, University of Graz , Heinrichstrasse 28, 8010 Graz, Austria.

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September 2017

Two tyrosine residues, Tyr-108 and Tyr-503, are responsible for the deprotonation of phenolic substrates in vanillyl-alcohol oxidase.

J Biol Chem 2017 09 17;292(35):14668-14679. Epub 2017 Jul 17.

From the Laboratory of Biochemistry, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands,

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September 2017

The VAO/PCMH flavoprotein family.

Arch Biochem Biophys 2017 10 30;632:104-117. Epub 2017 Jun 30.

Laboratory of Biochemistry, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands. Electronic address:

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October 2017

Crystal structures and atomic model of NADPH oxidase.

Proc Natl Acad Sci U S A 2017 06 12;114(26):6764-6769. Epub 2017 Jun 12.

Department of Biology and Biotechnology "L. Spallanzani," University of Pavia, 27100 Pavia, Italy;

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June 2017

Isolation and characterization of a thermostable F:NADPH oxidoreductase from .

J Biol Chem 2017 06 14;292(24):10123-10130. Epub 2017 Apr 14.

From the Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands,

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June 2017

Discovery and characterization of an F-dependent glucose-6-phosphate dehydrogenase (Rh-FGD1) from Rhodococcus jostii RHA1.

Appl Microbiol Biotechnol 2017 Apr 13;101(7):2831-2842. Epub 2016 Dec 13.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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April 2017

Engineering Cyclohexanone Monooxygenase for the Production of Methyl Propanoate.

ACS Chem Biol 2017 01 20;12(1):291-299. Epub 2016 Dec 20.

Molecular Enzymology Group, University of Groningen , Nijenborgh 4, 9747AG Groningen, The Netherlands.

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January 2017

P450 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations.

Appl Microbiol Biotechnol 2017 Mar 29;101(6):2319-2331. Epub 2016 Nov 29.

Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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March 2017

Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase.

Angew Chem Int Ed Engl 2016 12 22;55(51):15852-15855. Epub 2016 Nov 22.

Department of Biotechnology, University of Groningen, Nijenborgh 4, 9747AG, Groningen, The Netherlands.

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December 2016

Bacterial enzymes involved in lignin degradation.

J Biotechnol 2016 Oct 17;236:110-9. Epub 2016 Aug 17.

Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Electronic address:

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October 2016

Biocatalytic Properties and Structural Analysis of Eugenol Oxidase from Rhodococcus jostii RHA1: A Versatile Oxidative Biocatalyst.

Chembiochem 2016 07 7;17(14):1359-66. Epub 2016 Jun 7.

Molecular Enzymology, Groningen Biomolecular Sciences and, Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, NL.

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July 2016

Characterization of a chitinase from the cellulolytic actinomycete Thermobifida fusca.

Biochim Biophys Acta 2016 09 22;1864(9):1253-1259. Epub 2016 Apr 22.

University of Groningen, Molecular Enzymology Group, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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September 2016

Microbial Flavoprotein Monooxygenases as Mimics of Mammalian Flavin-Containing Monooxygenases for the Enantioselective Preparation of Drug Metabolites.

Drug Metab Dispos 2016 08 16;44(8):1270-6. Epub 2016 Mar 16.

Analytical Biochemistry, Groningen Research Institute of Pharmacy (T.G., H.P.P., R.B.), Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute (M.K., M.W.F.), and Interfaculty Mass Spectrometry Center (H.P.P.), University of Groningen, Groningen, the Netherlands.

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August 2016

Covalent immobilization of a flavoprotein monooxygenase via its flavin cofactor.

Enzyme Microb Technol 2016 Jan 30;82:138-143. Epub 2015 Sep 30.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands. Electronic address:

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January 2016

Erratum: Cloning, overexpression and biocatalytic exploration of a novel Baeyer-Villiger monooxygenase from Aspergillus fumigatus Af293.

AMB Express 2014 Dec 20;4(1):62. Epub 2014 Sep 20.

INTEQUI-CONICET, Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, CP 5700, San Luis, Argentina,

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December 2014

Lyophilization conditions for the storage of monooxygenases.

J Biotechnol 2015 Jun 26;203:41-4. Epub 2015 Mar 26.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Electronic address:

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June 2015

Catalases as biocatalysts in technical applications: current state and perspectives.

Appl Microbiol Biotechnol 2015 Apr 12;99(8):3351-7. Epub 2015 Mar 12.

Faculty of Chemistry, University of Belgrade, Studentskitrg 12-16, Belgrade, Serbia.

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April 2015

Expanding the substrate scope of chitooligosaccharide oxidase from Fusarium graminearum by structure-inspired mutagenesis.

Biotechnol Bioeng 2015 Jun 2;112(6):1074-80. Epub 2015 Mar 2.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

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June 2015

Finding the switch: turning a baeyer-villiger monooxygenase into a NADPH oxidase.

J Am Chem Soc 2014 Dec 1;136(49):16966-9. Epub 2014 Dec 1.

Molecular Enzymology Group, University of Groningen , Nijenborgh 4, 9747AG Groningen, The Netherlands.

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December 2014

The oxidation of thiols by flavoprotein oxidases: a biocatalytic route to reactive thiocarbonyls.

Angew Chem Int Ed Engl 2014 Nov 5;53(48):13206-9. Epub 2014 Oct 5.

Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3 (The Netherlands) http://www.bic.wur.nl.

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November 2014

Insights in the kinetic mechanism of the eukaryotic Baeyer-Villiger monooxygenase BVMOAf1 from Aspergillus fumigatus Af293.

Biochimie 2014 Dec 16;107 Pt B:270-6. Epub 2014 Sep 16.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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December 2014

Not so monofunctional--a case of thermostable Thermobifida fusca catalase with peroxidase activity.

Appl Microbiol Biotechnol 2015 Mar 17;99(5):2225-32. Epub 2014 Sep 17.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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March 2015

Synthesis of methyl propanoate by Baeyer-Villiger monooxygenases.

Chem Commun (Camb) 2014 Nov;50(86):13034-6

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Netherlands.

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November 2014

A rapid quantitative activity assay shows that the Vibrio cholerae colonization factor GbpA is an active lytic polysaccharide monooxygenase.

FEBS Lett 2014 Sep 7;588(18):3435-40. Epub 2014 Aug 7.

Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O. Box 5003, N-1432 Aas, Norway. Electronic address:

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September 2014

Kinetic mechanism of putrescine oxidase from Rhodococcus erythropolis.

FEBS J 2014 Oct 15;281(19):4384-93. Epub 2014 Aug 15.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.

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October 2014

Turning a monocovalent flavoprotein into a bicovalent flavoprotein by structure-inspired mutagenesis.

Bioorg Med Chem 2014 Oct 4;22(20):5621-7. Epub 2014 Jun 4.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Electronic address:

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October 2014

The taming of oxygen: biocatalytic oxyfunctionalisations.

Chem Commun (Camb) 2014 Nov;50(87):13180-200

DECHEMA Research Institute, Theodor-Heuss-Allee 25, 60486 Frankfurt am Main, Germany.

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November 2014

Enzyme-catalyzed oxidation of 5-hydroxymethylfurfural to furan-2,5-dicarboxylic acid.

Angew Chem Int Ed Engl 2014 Jun 6;53(25):6515-8. Epub 2014 May 6.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen (The Netherlands) http://www.rug.nl/staff/m.w.fraaije/research.

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June 2014

Stabilization of cyclohexanone monooxygenase by a computationally designed disulfide bond spanning only one residue.

FEBS Open Bio 2014 3;4:168-74. Epub 2014 Feb 3.

Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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March 2014

A fast, sensitive and easy colorimetric assay for chitinase and cellulase activity detection.

Biotechnol Biofuels 2014 Mar 10;7(1):37. Epub 2014 Mar 10.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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March 2014

Beyond the Protein Matrix: Probing Cofactor Variants in a Baeyer-Villiger Oxygenation Reaction.

ACS Catal 2013 ;3(12):3058-3062

Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.

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January 2013

Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688.

Appl Environ Microbiol 2014 Feb 22;80(3):1082-90. Epub 2013 Nov 22.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

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February 2014

Cascade reactions in multicompartmentalized polymersomes.

Angew Chem Int Ed Engl 2014 Jan 19;53(1):146-50. Epub 2013 Nov 19.

Radboud University Nijmegen, Institute for Molecules and Materials, Bio-organic Chemistry, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands).

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January 2014

Extending the substrate scope of a Baeyer-Villiger monooxygenase by multiple-site mutagenesis.

Appl Microbiol Biotechnol 2014 May 19;98(9):4009-20. Epub 2013 Nov 19.

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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May 2014

DyP-type peroxidases: a promising and versatile class of enzymes.

J Ind Microbiol Biotechnol 2014 Jan 9;41(1):1-7. Epub 2013 Nov 9.

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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January 2014

Cloning, overexpression and biocatalytic exploration of a novel Baeyer-Villiger monooxygenase from Aspergillus fumigatus Af293.

AMB Express 2013 06 14;3(1):33. Epub 2013 Jun 14.

INTEQUI-CONICET, Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, CP 5700, Argentina.

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June 2013

Special issue: Catalytic mechanisms by biological systems--introduction.

FEBS J 2013 Jul 3;280(13):2947. Epub 2013 Jun 3.

Molecular Enzymology Group, University of Groningen, Groningen, The Netherlands.

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July 2013

Flavoprotein oxidases: classification and applications.

Appl Microbiol Biotechnol 2013 Jun 3;97(12):5177-88. Epub 2013 May 3.

Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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June 2013

A generic, whole-cell-based screening method for Baeyer-Villiger monooxygenases.

J Biomol Screen 2013 Jul 27;18(6):678-87. Epub 2013 Mar 27.

Molecular Enzymology Group, University of Groningen, Groningen, The Netherlands.

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July 2013

Precursor of ether phospholipids is synthesized by a flavoenzyme through covalent catalysis.

Proc Natl Acad Sci U S A 2012 Nov 29;109(46):18791-6. Epub 2012 Oct 29.

Department of Biology and Biotechnology, University of Pavia, 27100 Pavia, Italy.

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November 2012

The enigmatic reaction of flavins with oxygen.

Trends Biochem Sci 2012 Sep 20;37(9):373-80. Epub 2012 Jul 20.

Department of Biochemistry and Center of Excellence in Protein Structure and Function, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand.

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September 2012

A stepwise approach for the reproducible optimization of PAMO expression in Escherichia coli for whole-cell biocatalysis.

BMC Biotechnol 2012 Jun 21;12:31. Epub 2012 Jun 21.

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.

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June 2012

Exploring the structural basis of substrate preferences in Baeyer-Villiger monooxygenases: insight from steroid monooxygenase.

J Biol Chem 2012 Jun 17;287(27):22626-34. Epub 2012 May 17.

Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.

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June 2012

Blending Baeyer-Villiger monooxygenases: using a robust BVMO as a scaffold for creating chimeric enzymes with novel catalytic properties.

Chem Commun (Camb) 2012 Apr 27;48(27):3288-90. Epub 2012 Jan 27.

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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April 2012

Hot or not? Discovery and characterization of a thermostable alditol oxidase from Acidothermus cellulolyticus 11B.

Appl Microbiol Biotechnol 2012 Jul 11;95(2):389-403. Epub 2012 Jan 11.

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, The Netherlands.

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July 2012

Functionalization of oxidases with peroxidase activity creates oxiperoxidases: a new breed of hybrid enzyme capable of cascade chemistry.

Chembiochem 2012 Jan 23;13(2):252-8. Epub 2011 Dec 23.

Laboratory of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

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January 2012

Risk assessment studies on succinate dehydrogenase inhibitors, the new weapons in the battle to control Septoria leaf blotch in wheat.

Mol Plant Pathol 2012 Apr 20;13(3):263-75. Epub 2011 Sep 20.

Rothamsted Research, Plant Pathology and Microbiology Department, Harpenden, Hertfordshire AL5 2JQ, UK.

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April 2012

Turning a riboflavin-binding protein into a self-sufficient monooxygenase by cofactor redesign.

Chem Commun (Camb) 2011 Oct 8;47(39):11050-2. Epub 2011 Sep 8.

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

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October 2011

Dynamic kinetic resolution of α-substituted β-ketoesters catalyzed by Baeyer-Villiger monooxygenases: access to enantiopure α-hydroxy esters.

Angew Chem Int Ed Engl 2011 Aug 15;50(36):8387-90. Epub 2011 Jul 15.

Dpto de Química Orgánica e Inorgánica, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo c/Julián Clavería 8, 33006 Oviedo, Spain.

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August 2011

Mapping the substrate binding site of phenylacetone monooxygenase from Thermobifida fusca by mutational analysis.

Appl Environ Microbiol 2011 Aug 1;77(16):5730-8. Epub 2011 Jul 1.

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

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August 2011