Publications by authors named "Marco Salinas"

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promoter DNA-methylation level and glucose metabolism in Ecuadorian women with Turner syndrome.

Horm Mol Biol Clin Investig 2020 Dec 22;42(2):159-165. Epub 2020 Dec 22.

Institute of Biomedicine Research, Central University of Ecuador, Quito, Ecuador.

Objectives: Reduced gene expression of in subjects with insulin resistance (IR) has been reported. Insulin resistance occurs early on the course of Turner syndrome (TS). The main objective of this study was to evaluate the relationship between promoter DNA methylation status in lymphocytes and insulin sensitivity and secretion in Ecuadorian females with TS.

Methods: We examined a cohort of 34 Ecuadorian patients with TS along with a sex-, age- and BMI-matched reference group. All subjects received a standard 75 g oral glucose tolerance test. Insulin resistance and secretion indices were calculated. The methylated DNA/unmethylated DNA ratio and mitochondrial content (mtDNA/nDNA ratio) were further determined.

Results: Notably, the DNA methylation level was significantly higher in TS subjects than the reference group and correlated with IR indices. Conversely, mitochondrial content was significantly lower in the study group than healthy controls and negatively correlated with the methylated DNA/unmethylated DNA ratio in TS individuals. promoter DNA methylation status contributed to 20% of the total variability in Homeostasis Model Assessment for Insulin Resistance (HOMA-IR) independently of BMI or age in TS subjects.

Conclusions: Our collective findings suggest that expression of and lower mitochondrial number affect the metabolic phenotype in TS subjects.
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December 2020