Publications by authors named "Marco Aurélio Guimarães"

12 Publications

  • Page 1 of 1

Total joint prosthesis for ankylosis after multiples condylar traumas.

Natl J Maxillofac Surg 2020 Jul-Dec;11(2):270-274. Epub 2020 Dec 16.

Federal University of Rio de Janeiro Dental School, Rio de Janeiro, Brazil.

Temporomandibular joint (TMJ) ankylosis is a disease commonly associated with facial trauma, especially in the condylar region. Described as a heterotopic bone growth that causes mouth opening limitation, it may occur in trauma cases misdiagnosed or mistreated. We present the case of a 37-year-old female, with decreased mouth opening after three traumas in the region of the right condyle by a motorcycle accident. We performed custom-made total joint replacement of the TMJ to rehabilitate the patient post arthroplasty. TMJ Concepts protocol was followed to perform the patient's rehabilitation. She is being followed since then by a physical therapist to regain the pre-pathosis status of mouth opening, speech, and mastication function.
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http://dx.doi.org/10.4103/njms.NJMS_91_20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051664PMC
December 2020

Corrigendum to "The impact of burial period on compact bone microstructure: histological analysis of matrix loss and cell integrity in human bones exhumed from tropical soil" [Forensic Sci. Int. 298C (2019) 384-392].

Forensic Sci Int 2019 09 24;302:109863. Epub 2019 Jun 24.

Department of Pathology, Escola Paulista de Medicina - Universidade Federal de São Paulo, São Paulo, SP, Brazil; Northumbria University, Newcastle Upon Tyne, United Kingdom. Electronic address:

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http://dx.doi.org/10.1016/j.forsciint.2019.06.021DOI Listing
September 2019

Corrigendum to "The impact of burial period on compact bone microstructure: histological analysis of matrix loss and cell integrity in human bones exhumed from tropical soil" [Forensic Sci. Int. 298C (2019) 384-392].

Forensic Sci Int 2019 09 24;302:109863. Epub 2019 Jun 24.

Department of Pathology, Escola Paulista de Medicina - Universidade Federal de São Paulo, São Paulo, SP, Brazil; Northumbria University, Newcastle Upon Tyne, United Kingdom. Electronic address:

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http://dx.doi.org/10.1016/j.forsciint.2019.06.021DOI Listing
September 2019

The impact of burial period on compact bone microstructure: Histological analysis of matrix loss and cell integrity in human bones exhumed from tropical soil.

Forensic Sci Int 2019 May 14;298:384-392. Epub 2019 Mar 14.

Department of Pathology, Escola Paulista de Medicina - Universidade Federal de São Paulo, São Paulo, SP, Brazil; Northumbria University, Newcastle Upon Tyne, United Kingdom. Electronic address:

Human bone histological analysis is a useful tool to assess post mortem diagenesis and to predict successful nuclear DNA typing of forensic material. This study is part of a series of studies developed by the authors intended to improve the understanding of post mortem diagenesis and to develop applications for DNA analysis of skeletal species from tropical soils, in order to optimize genetic and anthropological protocols. The aim of this study was to analyze the impact of burial period on the integrity of exhumed compact bone microstructure from tropical climate. In fragments of exhumed human femora from 39 individuals from the same cemetery (exhumed group) and 5 fresh femora from routine autopsies (control group), sections stained by hematoxylin-eosin were analyzed in order to measure bone microstructural integrity. We found that bone integrity index in exhumed group was negatively influenced by the period of burial (r = -0.37, p < 0.05) and highly significantly decreased (p < 0.0001) in comparison to control group. The period of burial and nitric acid decalcification time was positively correlated (r = 0.51; p < 0.01), leading to imply a bone petrification process during inhumation. Exhumed group showed higher level of matrix bone loss (p < 0.001), as expected, and 87% of cases analyzed were "tunneled" as described by Hackett. Bone integrity index and bone matrix tend to decrease in bones buried in tropical soil between 8-14 years of inhumation. This period is short if we consider cases in which there are preserved bones interred for longer periods in other environments. These data must be considered in cases where genetic identification of exhumed skeletons from tropical environment is required. The diagenesis in these bones and the variations of results found are discussed, clarifying some challenges for forensic laboratories, especially in DNA analysis.
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http://dx.doi.org/10.1016/j.forsciint.2019.03.008DOI Listing
May 2019

Validation of a standard forensic anthropology examination protocol by measurement of applicability and reliability on exhumed and archive samples of known biological attribution.

Forensic Sci Int 2017 Oct 7;279:241-250. Epub 2017 Sep 7.

Department of Pathology and Legal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address:

Forensic anthropology makes an important contribution to human identification and assessment of the causes and mechanisms of death and body disposal in criminal and civil investigations, including those related to atrocity, disaster and trafficking victim identification. The methods used are comparative, relying on assignment of questioned material to categories observed in standard reference material of known attribution. Reference collections typically originate in Europe and North America, and are not necessarily representative of contemporary global populations. Methods based on them must be validated when applied to novel populations. This study describes the validation of a standardized forensic anthropology examination protocol by application to two contemporary Brazilian skeletal samples of known attribution. One sample (n=90) was collected from exhumations following 7-35 years of burial and the second (n=30) was collected following successful investigations following routine case work. The study presents measurement of (1) the applicability of each of the methods: used and (2) the reliability with which the biographic parameters were assigned in each case. The results are discussed with reference to published assessments of methodological reliability regarding sex, age and-in particular-ancestry estimation.
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http://dx.doi.org/10.1016/j.forsciint.2017.08.015DOI Listing
October 2017

Validation of a standard forensic anthropology examination protocol by measurement of applicability and reliability on exhumed and archive samples of known biological attribution.

Forensic Sci Int 2017 Oct 7;279:241-250. Epub 2017 Sep 7.

Department of Pathology and Legal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address:

Forensic anthropology makes an important contribution to human identification and assessment of the causes and mechanisms of death and body disposal in criminal and civil investigations, including those related to atrocity, disaster and trafficking victim identification. The methods used are comparative, relying on assignment of questioned material to categories observed in standard reference material of known attribution. Reference collections typically originate in Europe and North America, and are not necessarily representative of contemporary global populations. Methods based on them must be validated when applied to novel populations. This study describes the validation of a standardized forensic anthropology examination protocol by application to two contemporary Brazilian skeletal samples of known attribution. One sample (n=90) was collected from exhumations following 7-35 years of burial and the second (n=30) was collected following successful investigations following routine case work. The study presents measurement of (1) the applicability of each of the methods: used and (2) the reliability with which the biographic parameters were assigned in each case. The results are discussed with reference to published assessments of methodological reliability regarding sex, age and-in particular-ancestry estimation.
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http://dx.doi.org/10.1016/j.forsciint.2017.08.015DOI Listing
October 2017

A forensic identification case and DPid - can it be a useful tool?

J Appl Oral Sci 2017 May-Jun;25(3):346-353

Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Estomatologia, Saúde Coletiva e Odontologia Legal, Ribeirão Preto, SP, Brasil.

Objective: The aim of this study was to show DPid as an important tool of potential application to solve cases with dental prosthesis, such as the forensic case reported, in which a skull, denture and dental records were received for analysis.

Material And Methods: Human identification is still challenging in various circumstances and Dental Prosthetics Identification (DPid) stores the patient's name and prosthesis information and provides access through an embedded code in dental prosthesis or an identification card. All of this information is digitally stored on servers accessible only by dentists, laboratory technicians and patients with their own level of secure access. DPid provides a complete single-source list of all dental prosthesis features (materials and components) under complete and secure documentation used for clinical follow-up and for human identification.

Results And Conclusion: If DPid tool was present in this forensic case, it could have been solved without requirement of DNA exam, which confirmed the dental comparison of antemortem and postmortem records, and concluded the case as a positive identification.
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http://dx.doi.org/10.1590/1678-7757-2016-0175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482259PMC
September 2017

Tissue Microarray Analysis Applied to Bone Diagenesis.

Sci Rep 2017 01 4;7:39987. Epub 2017 Jan 4.

Escola Paulista de Medicina-Universidade Federal de São Paulo, Department of Pathology, São Paulo 04023-062, Brazil.

Taphonomic processes affecting bone post mortem are important in forensic, archaeological and palaeontological investigations. In this study, the application of tissue microarray (TMA) analysis to a sample of femoral bone specimens from 20 exhumed individuals of known period of burial and age at death is described. TMA allows multiplexing of subsamples, permitting standardized comparative analysis of adjacent sections in 3-D and of representative cross-sections of a large number of specimens. Standard hematoxylin and eosin, periodic acid-Schiff and silver methenamine, and picrosirius red staining, and CD31 and CD34 immunohistochemistry were applied to TMA sections. Osteocyte and osteocyte lacuna counts, percent bone matrix loss, and fungal spheroid element counts could be measured and collagen fibre bundles observed in all specimens. Decalcification with 7% nitric acid proceeded more rapidly than with 0.5 M EDTA and may offer better preservation of histological and cellular structure. No endothelial cells could be detected using CD31 and CD34 immunohistochemistry. Correlation between osteocytes per lacuna and age at death may reflect reported age-related responses to microdamage. Methodological limitations and caveats, and results of the TMA analysis of post mortem diagenesis in bone are discussed, and implications for DNA survival and recovery considered.
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http://dx.doi.org/10.1038/srep39987DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209720PMC
January 2017

[Forgotten patriots].

Hist Cienc Saude Manguinhos 2010 Jun;17(2):551-4

Centro de Desenvolvimento Sustentável, Universidade de Brasília.

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http://dx.doi.org/10.1590/s0104-59702010000200021DOI Listing
June 2010

A study of the effects of pinealectomy on intestinal cell proliferation in infant newborn rats.

Acta Cir Bras 2006 Jan-Feb;21(1):16-20. Epub 2006 Feb 13.

University Hospital, FMRP, USP, São Paulo, Brazil.

Purpose: Study the proliferation rate of jejunum and large intestine crypt epithelial cells, in rats pinealectomized immediately after birth.

Methods: Twenty-four male Wistar rats were distributed into two groups: Acute group (n=12) and Chronic group (n=12). Six animals of each group were operated for removal of the pineal gland (pinealectomy-PnX), and other six were controls (sham pinealectomy-C). Animals from acute and chronic group were sacrificed 15 and 90 days after the surgery, respectively.

Results: In acute group, pinealectomy of new-born rats has not caused significant alteration in cell proliferation (PnX=58.77+/-1.77 and C=60.88+/-1.10 in the descending colon/PnX=31.56+/-0.45 and C=31.73+/-0.47 in the proximal jejunum) and in crypt cell population (PnX=24.92+/-4.82 and C=23.60+/-2.48 in the descending colon/PnX=39.92+/-3.49 and C=44.32+/-5.56 in the proximal jejunum). However, in chronic group there was an uprising crypt cell production per crypt in the proximal jejunum (PnX=57.54+/-2.19 and C=47.19+/-7.3)and in the descending colon (PnX=37.78+/-2.22 and C=17.92+/-2.28).

Conclusion: As the increase of intestinal crypts epithelial cells in chronic group is a carcinogenesis predetermining factor, the understanding of the interaction between pineal gland and this event has great importance.
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http://dx.doi.org/10.1590/s0102-86502006000100005DOI Listing
August 2006

Hyperplasia of the colonic neuroendocrine cells after pinealectomy in rats. The new evidence for the existence of connections between the distant parts of the diffuse neuroendocrine system.

Neuro Endocrinol Lett 2005 Oct;26(5):511-4

Department of Pathology, Ribeirão Preto School of Medicine, University of São Paulo, Brazil.

Objectives: The diffuse neuroendocrine system (DNES) participates in the systemic homeostasis and may work as a unified system with integrated functions but anatomically disperse. However, the mechanisms that are involved in the integration of the distant parts of the DNES are poorly known. This study is aimed on the effects of a pinealectomy on the population of the neuroendocrine cells (NECs) in the colon of rats.

Methods: A group of seven newborn rats and one of adult rats were submitted to a pinealectomy. The animals were sacrificed after 15 days and 90 days, respectively. The control groups of adult and newborn rats were subjected to a 'sham surgery' and then sacrificed after similar periods of time. Paraffin-included sections of the colon samples were stained by the Churukian-Schenk method in order to selectively stain the NECs. The NEC index was estimated by the expression: iNEC = NECs population per crypt/Total cell population of the crypt. The results were expressed as median and absolute range, and the statistical significance was demonstrated by the Mann-Whitney (U) test.

Results: The morphometric analysis showed a significant increase in the number of colonic NECs of the adult rats subjected to a pinealectomy when compared to the controls. No statistical difference was observed between the iNECs of the newborn groups of rats.

Conclusion: This finding suggests the existence of regulatory mechanisms that integrate distant parts of the DNES, by adapting the population of the endocrine cells after the ablation of an important component of the system. Further studies on the mechanisms involved in the integration of the DNES may be facilitated by the simple experimental model that we propose.
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October 2005

Prepubertal administration of estradiol valerate disrupts cyclicity and leads to cystic ovarian morphology during adult life in the rat: role of sympathetic innervation.

Endocrinology 2003 Oct 19;144(10):4289-97. Epub 2003 Jun 19.

Department of Physiology, Universidad de Sao Paulo, Brazil.

Administration of estradiol valerate (EV) to adult rats leads to anovulation and cystic ovarian morphology. Sympathetic ovarian nerve denervation (SONX) overcomes this disruption. In this study, we determined whether EV administration to juvenile rats prevents achievement of reproductive competence, disrupts cyclicity, and whether this programming is facilitated via activation of the sympathetic nerve input to the ovary. Prepubertal rats were administered 2 mg EV in corn oil or corn oil alone. One half of the animals from each group underwent SONX on d 71 of life. Rats were euthanized on d 91 for determination of serum gonadotropins, progesterone, Delta4 androstenedione, and estradiol concentrations, ovarian norepinephrine (NE), and 3beta-hydroxysteroid dehydrogenase (3beta-HSD) activities and ovarian dynamics. Results revealed that EV administration during juvenile period advanced pubertal onset, suppressed circulating LH, FSH, and Delta4 androstenedione, increased ovarian NE, estradiol, and 3beta-HSD activities, disrupted ovarian dynamics evidenced as absent corpus luteum and presence of ovarian cysts and culminated in anovulation. SONX restored cyclicity in these animals, normalized LH, estradiol, ovarian 3beta-HSD activities, and ovarian dynamics as evidenced by the disappearance of ovarian cysts and appearance of corpus luteum and restored corpus luteum function. These findings provide evidence that EV exposure during juvenile life leads to long-lasting deleterious reproductive consequences via activation of the sympathetic ovarian nerve.
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http://dx.doi.org/10.1210/en.2003-0146DOI Listing
October 2003