Publications by authors named "Marcelo Belini Dalio"

3 Publications

  • Page 1 of 1

Assessment of gait dynamics in rats submitted to limb ischemia.

Acta Cir Bras 2011 Dec;26(6):490-5

Division of Vascular and Endovascular Surgery, Department of Surgery and Anatomy, FMRP, USP, Ribeirao Preto, SP, Brazil.

Purpose: To describe a method for the assessment of gait dynamics in rats submitted to limb ischemia.

Methods: Twenty-four male Wistar rats (150-160g) were used. Twelve animals were submitted to limb ischemia by ligation of the common left iliac artery (ischemic group: n = 12); and a sham-operated group was used as control (n=12). After a recovery period of 6 weeks, gait dynamics was assessed by counting the complete footprints and the number of hindlimb-floor contacts during a treadmill test for five minutes at a speed of 12 m.min(-1) and angulation of 15°. The number of contacts of the left hindlimb was divided by the right hindlimb values (LRR) for group comparisons. Ischemic disability was quantified by comparing the area under curve (AUC) created by plotting each contact versus time for each hindlimb. The left hindlimb ischemic disability index (LHDI), which was compared between groups, was defined by the formula: LHDI = (1- AUC (left) / AUC (right)) x 100.

Results: Surgery was well tolerated by all animals. Rats did not suffer tissue loss or ulcerations. Complete footprint LRR was 0.3 ± 0.08 for the ischemic group and 1.3 ± 0.9 for controls (p=0.0043). Number of contacts LLR was 0.5 ± 0.2 for the ischemic group and 1.0 ± 0.1 for the control group (p=0.0051). LHDI was 56.83 ± 10.67 for the ischemic group and 2.50 ± 13.10 for the control group (P = 0.031).

Conclusion: Assessment of gait dynamics in rats submitted to limb ischemia could be done by footprint analysis and hindlimb contact recording during a treadmill test.
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http://dx.doi.org/10.1590/s0102-86502011000600014DOI Listing
December 2011

A study of the effects of pinealectomy on intestinal cell proliferation in infant newborn rats.

Acta Cir Bras 2006 Jan-Feb;21(1):16-20. Epub 2006 Feb 13.

University Hospital, FMRP, USP, São Paulo, Brazil.

Purpose: Study the proliferation rate of jejunum and large intestine crypt epithelial cells, in rats pinealectomized immediately after birth.

Methods: Twenty-four male Wistar rats were distributed into two groups: Acute group (n=12) and Chronic group (n=12). Six animals of each group were operated for removal of the pineal gland (pinealectomy-PnX), and other six were controls (sham pinealectomy-C). Animals from acute and chronic group were sacrificed 15 and 90 days after the surgery, respectively.

Results: In acute group, pinealectomy of new-born rats has not caused significant alteration in cell proliferation (PnX=58.77+/-1.77 and C=60.88+/-1.10 in the descending colon/PnX=31.56+/-0.45 and C=31.73+/-0.47 in the proximal jejunum) and in crypt cell population (PnX=24.92+/-4.82 and C=23.60+/-2.48 in the descending colon/PnX=39.92+/-3.49 and C=44.32+/-5.56 in the proximal jejunum). However, in chronic group there was an uprising crypt cell production per crypt in the proximal jejunum (PnX=57.54+/-2.19 and C=47.19+/-7.3)and in the descending colon (PnX=37.78+/-2.22 and C=17.92+/-2.28).

Conclusion: As the increase of intestinal crypts epithelial cells in chronic group is a carcinogenesis predetermining factor, the understanding of the interaction between pineal gland and this event has great importance.
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http://dx.doi.org/10.1590/s0102-86502006000100005DOI Listing
August 2006

A new experimental model to study preneoplastic lesions in achalasia of the esophagus.

Acta Cir Bras 2005 Nov-Dec;20(6):418-21. Epub 2005 Nov 8.

FMRP, University of São Paulo, Brazil.

Purpose: Develop an experimental model to study esophageal preneoplastic lesions induced by diethylnitrosamine in rats with achalasia.

Methods: Male Wistar rats were divided into four groups: control--C (n=8); rats with megaesophagus--B (n=8); rats treated with DEN--D (n=15) and rats with megaesophagus plus DEN--BD (n=15). Megaesophagus can be experimentally obtained in rats by topical application of benzalkonium chloride. The morphology and PCNA labeling index of the epithelium were evaluated.

Results: The morphometric analysis showed an increase in epithelial thickness in the animals of group BD (2166+/-1012 mm2) when compared to the other groups (C = 878+/-278 mm2; B = 1746+/-144 mm2 and D = 1691+/-697 mm2), mainly due to basal layer hyperplasia, besides an increase in the keratin of the superficial layer. The PCNA labeling index in the basal layer was significantly higher in the group BD (0.695+/-0.111) when compared to the other groups (C = 0.490+/-0.132; B = 0.512+/-0.215 and D = 0.477+/-0.198).

Conclusions: Our data confirm in an experimental model the previous observation in humans of increased epithelial cell proliferation during the esophageal carcinogenic process in achalasia and may be useful to further studies on the mechanisms of the esophageal carcinogenesis and the the design of follow-up endoscopic studies for patients with achalasia.
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http://dx.doi.org/10.1590/s0102-86502005000600004DOI Listing
July 2006