Publications by authors named "Mara Silvia Carvalhaes"

7 Publications

  • Page 1 of 1

Analysis of Spleen Cells in Susceptible and Resistant Mice with Experimental Lagochilascariosis.

ISRN Parasitol 2013 12;2013:180652. Epub 2012 Sep 12.

Department of Microbiology, Immunology, Parasitology and Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.

Lagochilascariosis is an emerging parasitic disease caused by the helminth Lagochilascaris minor. The experimental mouse model has been used to study the immune response against L. minor infection. In the present work, immunohistochemistry analysis of spleen cells populations was evaluated in susceptible (C57BL/6) and resistant (BALB/c) mice experimentally infected with L. minor. The BALB/c mice exhibited increased spleen cell indexes as follows: F4/80+ at 100 days after infection (DPI), CD4+ at 100 and 250 DPI, CD8+ at 35 and 100 DPI, and CD19+ at 100, 150, and 250 DPI. In the spleens of the infected C57BL/6 mice, increased indexes of the following spleen cells were observed: F4/80+ cells at 250 DPI, CD4+ cells at 150 DPI, CD8+ cells at 35, 150, and 250 DPI, and CD19+ cells at 150 to 250 DPI. The index of spleen cells confirmed the differences between the control and infected groups at several time points following the infection. These data demonstrate an association between a preferential increase in the number of CD4+ and CD19+ spleen cells and resistance to experimental lagochilascariosis in BALB/c mice and between a preferential increase in the number of CD8+ spleen cells and susceptibility in C57BL/6 mice.
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http://dx.doi.org/10.5402/2013/180652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890894PMC
June 2016

Lagochilascaris minor: Susceptibility and Resistance to Experimental Infection in Mice Is Independent of H-2 Haplotype and Correlates with the Immune Response in Immunized Animals.

J Parasitol Res 2010 22;2010. Epub 2010 Jun 22.

Laboratory of Immunochemistry, Butantan Institute, 05503-900 São Paulo, Brazil.

Recently, we demonstrated that C57BL/6 mice are more susceptible to experimental lagochilascariosis than BALB/c mice. To investigate the pattern of infection and the role of the genetic background on susceptibility to infection, we studied experimental lagochilascariosis in H-2(a) identical B10.A and A/J mice. Infected B10.A mice had a lower survival ratio and more severe lesions in the lungs than did A/J mice. Splenocytes of A/J mice immunized with the crude extract of the parasite showed increased proliferation and produced a higher level of interleukin 10 and interferon-gamma in the presence of CE or concanavalin A when compared to B10.A mice. This suggests that resistance of A/J mice may be due to less severe lesions in lungs and other organs and a better immune response to parasite antigens. This paper provides evidence that major histocompatibility complex haplotype does not influence the survival to experimental infection with L. minor.
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http://dx.doi.org/10.1155/2010/610457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2915754PMC
July 2011

Experimental lagochilascariosis in X-chromosome-linked immunodeficient mice.

Rev Soc Bras Med Trop 2009 Jul-Aug;42(4):381-5

Department of Microbiology, Immunology, Parasitology and Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.

Lagochilascaris minor is the etiological agent of lagochilascariosis, a disease that affects the neck region and causes exudative abscesses, with eggs, adult parasites and L3/L4 larvae in the purulent exudates. Mice are now considered to be intermediate hosts for the parasite. To determine the pattern of infection in B1 cell-deficient mice, experimental lagochilascariosis was studied in BALB/c and X-chromosome-linked immunodeficient (xid) mice. BALB.xid-infected mice showed lower numbers of larvae. Third-stage larvae, fourth-stage larvae and adult parasites were found in both strains. BALB/c mice produced IgM, IgG, IgA and IgE against the crude extract and secreted/excreted antigens of the parasite. On the other hand, BALB.xid mice did not produce IgM and produced lower levels of IgG and IgA, and similar quantities of IgE.
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http://dx.doi.org/10.1590/s0037-86822009000400005DOI Listing
March 2010

Lagochilascaris minor: antibody production in experimentally infected mice.

Rev Soc Bras Med Trop 2009 May-Jun;42(3):325-8

Department of Microbiology, Immunology, Parasitology and Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.

Lagochilascaris minor is the causative agent of lagochilascariosis, a disease that affects the neck region and causes festering abscesses, with eggs, adult parasites and L3/L4 larvae within the purulent exudates. Today, mice are considered to be intermediate hosts for the parasite. C57BL/6 mice produce immunoglobulin IgM, IgA and IgG against the crude extract of the parasite; on the other hand, antibodies produced against the secreted/excreted antigens of Lagochilascaris minor present lower levels of IgM, IgA and IgG. This is the first description of antibody detection against different antigens of Lagochilascaris minor.
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http://dx.doi.org/10.1590/s0037-86822009000300016DOI Listing
November 2009

[Thereza Liberman Kipnis (*1938 2008)].

Rev Soc Bras Med Trop 2008 Sep-Oct;41(5):546

Universidade Federal de Goiás.

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http://dx.doi.org/10.1590/s0037-86822008000500024DOI Listing
April 2009

[Hematological, serum biochemical and serological profile of Felis domesticus with experimental lagochilascariosis].

Rev Soc Bras Med Trop 2008 Sep-Oct;41(5):496-501

Departamento de Imunologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO.

The present study evaluated the hemogram, different proteins, plasma enzymes, serum enzymes and specific antibody production of Felis domesticus experimentally infected by Lagochilascaris minor. The infected animals were seen to present increased total leukocytes (particularly eosinophils), decreased platelet counts, increased aspartate-aminotransferase and alanine-aminotransferase and, especially, the presence of specific IgG antibodies against antigens of the parasite. The reaction with crude extract of adult parasites was shown to be more specific, thereby enabling serum discrimination between the animals: non-infected, infected with other parasites and infected with lagochilascariosis. This is the first description of the standardization of a serological reaction for diagnosing lagochilascariosis in Felis domesticus.
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http://dx.doi.org/10.1590/s0037-86822008000500012DOI Listing
April 2009

Lagochilascaris minor: experimental infection of C57BL/6 and BALB/c isogenic mice reveals the presence of adult worms.

Exp Parasitol 2008 Jul 26;119(3):325-31. Epub 2008 Mar 26.

Department of Microbiology, Immunology, Parasitology and Pathology (DMIPP), Institute of Tropical Pathology and Public Health (IPTSP), Federal University of Goiás (UFG), Goiânia, Goiás, Brazil.

The nematode Lagochilascaris minor is the causative agent of lagochilascariosis, a human disease that affects the neck region causing exudative abscesses with eggs, larvae, and adult parasites. Mice are currently considered intermediate hosts for the parasite. To determine the pattern of infection and the possibility of mice as definitive hosts for L. minor, experimental lagochilascariosis was studied in two distinct isogenic mouse strains: BALB/c and C57BL/6. Our results indicate that BALB/c mice are more resistant to L. minor infection than C57BL/6, having less intense lesions in the lungs, a lower number of nodules with encysted larvae and fewer adult worms, and displaying a higher serum level of IFN gamma. Both mouse strains had low levels of serum IL-10. We also observed adult parasites in both mouse strains, raising the possibility that mice are definitive hosts of L. minor. This is the first description of adult parasite development of L. minor in mice.
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http://dx.doi.org/10.1016/j.exppara.2008.03.009DOI Listing
July 2008