Publications by authors named "Mao Zhao"

19 Publications

  • Page 1 of 1

Traditional Chinese medicine for Helicobacter pylori infection: A protocol for a systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jan;100(3):e24282

Chengdu University of Traditional Chinese Medicine, School of Basic Medical Sciences, Chengdu, China.

Background: Helicobacter pylori (Hp) is the only bacterium in the stomach. It is characterized by its ability to adhere to gastric mucosa and cause a series of pathological changes in the gastric mucosa. Modern research shows that Hp is an important pathogenic factor for chronic gastritis, gastroduodenal ulcer, and gastric cancer. Triple, quadruple, and triple combinations of antibacterial drugs, proton pump inhibitors, and bismuth aluminate preparations have been developed in modern medical research. Sequential therapy is used to treat Hp, but antibiotic resistance and repeated infections still exist. A large number of clinical trials have proved that traditional Chinese medicine has a good therapeutic effect on Hp. In this systematic review, we aim to evaluate the efficacy and safety of traditional Chinese medicine in the treatment of Hp.

Methods And Analysis: We will search for publications from Web of Science, PubMed, Science Direct, Wan Fang Data Knowledge Service Platform, Chinese Biomedical Literature Database (CBM), Chinese Scientific Journal Database (VIP database), China National Knowledge Infrastructure (CNKI) and EMBASE, which should be published from inception to December 2020. Two researchers will independently perform the selection of the studies, data extraction, and synthesis. The Cochrane Risk of Bias Tool will be used to evaluate the risk of bias in the randomized controlled trials. Statistical analysis will be performed by using the Cochrane Review Manager (RevMan 5.3) software. The I2 test will be used to identify the extent of heterogeneity. We will use the Egger funnel chart to evaluate possible publication biases, in addition, when possible we will perform a subgroup/meta-regression analysis. The strength of the evidence will be assessed according to the Grading of Recommendations Assessment, Development, and Evaluation (GRADE).

Results And Conclusions: This study will systematically evaluate the efficacy of traditional Chinese medicine in the treatment of Hp infection, and provide evidence for the clinical application of this treatment. The results of the research will be published in a peer-reviewed journal.

Ethics: This systematic review will evaluate the efficacy of traditional Chinese medicine for Hp infection. Because all data used in this systematic review and meta-analysis have been published, this review does not require ethical approval.

Trial Registration Number: INPLASY2020120057.
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http://dx.doi.org/10.1097/MD.0000000000024282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837890PMC
January 2021

Efficacy and safety evaluation of acupuncture therapy for patients with salpingitis in IVF-ET: A protocol of systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jan;100(2):e24015

Chengdu University of Traditional Chinese Medicine, School of Basic Medical Sciences.

Background: As an alternative for salpingitis in IVF-ET, acupuncture has gradually attracted the attention of clinicians based on the theory of syndrome differentiation and treatment of Chinese traditional medicine. However, due to the lack of evidence-based medical evidence, the author designed the program to evaluate the effectiveness and safety of acupuncture.

Methods: From the beginning to August 2020, 7 electronic databases will be searched. Two of our researchers will independently conduct research selection, data extraction, and risk assessment of bias. We will use Review Manager 5.3 software for meta-analysis and heterogeneity assessment. In addition, we will use the grading of recommendations assessment, development, and evaluation to evaluate the evidence quality.

Results: This study will demonstrate an evidence-based review of acupuncture for salpingitis in IVF-ET.

Conclusion: The study will provide clear evidence to assess the effectiveness and side effects of acupuncture for salpingitis in IVF-ET.

Trial Registration Number: INPLASY2020110125.
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http://dx.doi.org/10.1097/MD.0000000000024015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7808486PMC
January 2021

The efficacy of Tuina for asthma: A protocol for a systematic review and meta-analysis.

Medicine (Baltimore) 2020 Dec;99(52):e23912

School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Background: Asthma is one of the most common chronic diseases in the world, with ∼100 million asthma patients worldwide. China has become one of the countries with the highest asthma death rate in the world. Asthma is a chronic airway inflammatory disease. Patients with this disease may have symptoms such as cough, wheezing, and difficulty breathing. For many years, Western medicine has mainly used anti-inflammatory, anti-bronchial spasm, asthma, cough, and oxygen to treat this disease, but the effect is not good. Tuina is a common treatment for asthma in China. But at present, there is no systematic evaluation report on its therapeutic effectiveness and safety. This protocol aims to reveal the efficacy and safety of Tuina for treating asthma.

Methods: The following databases will be searched by electronic methods: PubMed, EBASE, WHO International Clinical Trials Registry Platform, Embase, the Chinese Biomedical Literature Database (CBM), Wan-fang Data (WANFANG), the China National Knowledge Infrastructure (CNKI), and other sources from inception to November 2020. Bias risk, subgroup analysis, data synthesis, and meta-analyses will be assessed with RevMan V.5.3 software if the data is met inclusion conditions.

Results: This study will present a quality evidence of Tuina for the treatment of astma patients.

Conclusion: The systematic review will present reliable evidence to judge whether or not Tuina is a safe and effective intervention for asthma patients. International Platform of Registered Systematic Review and Meta-Analysis Protocols (INPLASY) registration number: INPLASY2020110100.
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http://dx.doi.org/10.1097/MD.0000000000023912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769308PMC
December 2020

Chinese herbal medicine for previous cesarean scar defect: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Dec;99(50):e23630

School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Background: Previous cesarean scar defect (PCSD) is a gynecological disease that can cause bleeding after intercourse, prolonging menstrual period, intermenstrual bleeding, dysmenorrhea, and even lead to infertility. Chinese herbal medicine plays an important role in the treatment of gynecological diseases in China and East Asia. This study aims to assess the efficacy and safety of Chinese herbal medicine for PCSD.

Methods: We search the following databases: PubMed, the Cochrane Library, Chinese Biomedical Literature Database (CB), Chinese Science and Technique Journals Database (VIP), EMBASE, Chinese National Knowledge Infrastructure Database (CNKI), and the Wanfang Database. Other sources will also be searched like Google Scholar and gray literature. All databases mentioned above are searched from the start date to the latest version. Randomized controlled trials will be included which recruiting PCSD participants to assess the efficacy and safety of Chinese herbal medicines against controls (placebo or other therapeutic agents). Primary outcomes will include the size of PCSD, menstrual cycle, menstrual phase, menstrual volume, duration of disease, security index. Two authors will independently scan the searched articles, extract the data from attached articles, and import them into Endnote X8 and use Microsoft Excel 2013 to manage data and information. We will assess the risk of bias by Cochrane tool of risk of bias. Disagreements will be resolved by consensus or the participation of a third party. All analysis will be performed based on the Cochrane Handbook for Systematic Reviews of Interventions. The meta-analysis in this review will use RevMan 5.3 software.

Results: The study aims to evaluate the efficacy and safety of the treatment that Chinese herbal medicine for PCSD.

Conclusion: This study of the meta-analysis could provide evidence for clinicians and help patients to make a better choice.

Inplasy Registration Number: INPLASY202090080.
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http://dx.doi.org/10.1097/MD.0000000000023630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738138PMC
December 2020

Uncoupling protein-2 regulates M1 macrophage infiltration of gingiva with periodontitis.

Cent Eur J Immunol 2020 ;45(1):9-21

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.

Periodontitis is an inflammatory disease accompanied by alveolar bone loss. Moreover, M1 macrophages play a critical role in the development of periodontal disease. Uncoupling protein-2 (UCP2) is a mitochondrial transporter protein that controls M1 macrophage activation by modulating reactive oxygen species (ROS) production. We investigated the role of UCP2 in M1 macrophage infiltration in gingival tissues with periodontitis. We found that the expression of UCP2 was upregulated in M1 macrophages infiltrating human periodontal tissues with periodontitis. Macrophage-specific knockout of UCP2 could increase the infiltration of macrophage and exacerbate inflammatory response in a mouse gingiva affected with periodontitis, induced by Porphyromonas gingivalis-LPS (Pg-LPS) injection. The loss of UCP2 may contribute to the enhanced abilities of proliferation, migration, pro-inflammatory cytokine secretion, and ROS production in Pg-LPS-treated macrophages. Our results indicate that UCP2 has an important role in M1 macrophage polarization in the periodontal tissue with periodontitis. It might be helpful to provide theoretical basis for design of new therapeutic strategies for periodontitis.
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http://dx.doi.org/10.5114/ceji.2020.94664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226558PMC
January 2020

Effect of resting heart rate on the risk of all-cause death in Chinese patients with hypertension: analysis of the Kailuan follow-up study.

BMJ Open 2020 03 10;10(3):e032699. Epub 2020 Mar 10.

Department of Cardiology, Kailuan General Hospital, Tangshan, Hebei, China.

Objective: Previous studies have shown that an elevated heart rate is associated with a higher risk of cardiovascular events. This study aimed to prospectively examine the relationship between resting heart rate (RHR) and all-cause mortality in Chinese patients with hypertension.

Design: An observational, prospective and population-based cohort study.

Setting: The Kailuan cohort study was conducted in Tangshan City in northern China.

Participants: We enrolled 46 561 patients who did not receive beta-blocker treatment and were diagnosed with hypertension for the first time during an employee health examination in Kailuan Group in 2006 and 2008.

Outcome: The primary outcome of this study was all-cause mortality.

Methods: The patients in this study were followed for 9.25±1.63 years. All patients were followed up face to face every 2 years. According to the distribution of RHR in the study population, RHR was categorised into five groups on the basis of quintiles: Q1: RHR ≤68 beats per minute (bpm); Q2: RHR >68 and ≤72 bpm; Q3: RHR >72 and ≤76 bpm; Q4: RHR >76 and ≤82 bpm; Q5: RHR >82 bpm. Cox proportional hazards model, which was adjusted for traditional risk factors, was used.

Results: During follow-up, 4751 deaths occurred. After adjustment for potential confounders, restricted cubic spline regression showed that the risk of all-cause mortality increased with heart rate. In multivariate Cox regression analyses adjusted for age, sex and major covariates, the HR for all-cause mortality was 1.31 (95% CI 1.27 to 1.33) in the highest quintile group (Q5) compared with the lowest quintile group (Q1).

Conclusion: An increase in RHR is a long-term risk factor of all-cause mortality in Chinese patients with hypertension.

Trial Registration Number: ChiCTR-TNC-11001489.
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http://dx.doi.org/10.1136/bmjopen-2019-032699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066611PMC
March 2020

Icariin protects against glucocorticoid induced osteoporosis, increases the expression of the bone enhancer DEC1 and modulates the PI3K/Akt/GSK3β/β-catenin integrated signaling pathway.

Biochem Pharmacol 2017 07 11;136:109-121. Epub 2017 Apr 11.

Department of Pharmacology, Nanjing Medical University, Nanjing 210029, China. Electronic address:

Osteoporosis is a serious public health concern worldwide. Herba epimedii has been used for centuries and even thousands of years to treat osteoporotic conditions. Icariin, a flavonol glycoside, is one of the major active ingredients. In this study, we have shown that icariin protected against glucocorticoid-induced osteoporotic changes in SaoS-2 cells and mice. We have also shown that dexamethasone (a glucocorticoid) suppressed and icariin induced DEC1, a structurally distinct helix-loop-helix protein. DEC1 overexpression promoted whereas DEC1 knockdown decreased osteogenic activity. Likewise, DEC1 overexpression and knockdown inversely regulated the expression of β-catenin and PIK3CA, an essential player in the Wnt/β-catenin and PI3K/Akt signaling pathways, respectively. Interestingly, DKK1, an inhibitor of Wnt/β-catenin signaling inhibitor, and LY294002, an inhibitor of PI3K/Akt signaling, abolished the induction of DEC1 by icariin. It is established that these two pathways are interconnected by the phosphorylation status of GSK3β. Dexamethasone decreased but icariin increased GSK3β phosphorylation. Finally, DEC1 deficient mice developed osteoporotic phenotypes. Taken together, it is concluded that DEC1 likely supports the action of icariin against glucocorticoid induced osteoporosis with an involvement of the PI3K/Akt/GSK3β/β-catenin integrated signaling pathway.
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http://dx.doi.org/10.1016/j.bcp.2017.04.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316728PMC
July 2017

An inflammatory myofibroblastic tumor of the small intestine in a 15-year-old girl.

Turk J Gastroenterol 2017 Jan 23;28(1):71-72. Epub 2016 Dec 23.

Department of General Medicine, Yantai Yuhuangding Hospital, Shandong Province, PR China.

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http://dx.doi.org/10.5152/tjg.2016.0513DOI Listing
January 2017

Fluoxetine reduces CES1, CES2, and CYP3A4 expression through decreasing PXR and increasing DEC1 in HepG2 cells.

Xenobiotica 2016 4;46(5):393-405. Epub 2015 Sep 4.

a Department of Pharmacology , Nanjing Medical University , Nanjing , China and.

1. This study investigated the mechanisms of the decreases of carboxylesterases (CES) and cytochrome P4503A4 (CYP3A4) and the enzymatic activities induced by fluoxetine (FLX) in HepG2 cells. We found that FLX decreased the carboxylesterase 1 (CES1) and carboxylesterase 2 (CES2) expression and the hydrolytic activity. 2. FLX decreased the pregnane X receptor (PXR) expression which regulated the target genes such as CYP3A4, whereas increased the differentiated embryonic chondrocyte-expressed gene 1 (DEC1) expression. 3. FLX repressed the PXR at transcriptional level. 4. Overexpression of PXR alone increased the expression of CES1, CES2, and CYP3A4 and attenuated the decreases of CES1, CES2, and CYP3A4 induced by FLX. On the contrary, knockdown of PXR alone decreased the expression of CES1, CES2, and CYP3A4 and almost abolished the decreases of CES1, CES2, and CYP3A4 induced by FLX. 5. Knockdown of DEC1 alone increased the expression of PXR and CYP3A4 and almost abolished the decreases of CES1, CES2, and CYP3A4 induced by FLX. 6. Taken together, the decreases of CES and CYP3A4 expression and enzymatic activities induced by FLX are through decreasing PXR and increasing DEC1 in HepG2 cells.
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http://dx.doi.org/10.3109/00498254.2015.1082209DOI Listing
November 2016

Differentiated embryonic chondrocytes 1 expression of periodontal ligament tissue and gingival tissue in the patients with chronic periodontitis.

Arch Oral Biol 2015 Apr 25;60(4):517-25. Epub 2014 Dec 25.

Department of Pharmacology, Nanjing Medicine University, Nanjing, China. Electronic address:

Objective: To evaluate the DEC1 expression of periodontal ligament tissue and gingival tissue in the patients with chronic periodontitis.

Methods: 20 non-smoking patients with chronic periodontitis and 20 healthy individuals were enrolled. Periodontal ligament tissue and gingival tissue samples from healthy subjects were collected during teeth extraction for orthodontic reason or the third molar extraction. The parallel samples from patients with chronic periodontitis were obtained during periodontal flap operations or teeth extraction as part of periodontal treatment. The DEC1 expression and the alkaline phosphatase (ALP) activity of both the periodontal ligament tissue and gingival tissue were determined by Western blot, Immunohistochemistry and ALP Detection Kit.

Results: The DEC1 expression of periodontal ligament tissue in the patients with chronic periodontitis decreased significantly along with the decreased ALP activity. On the contrary, the DEC1 expression of gingival tissue in the patients with chronic periodontitis increased significantly. Further study found that the DEC1 expression of gingival tissue increased mainly in the suprabasal layer of gingival epithelial cells but decreased in the gingival connective tissue of the patients with chronic periodontitis.

Conclusion: The DEC1 expression decreases in the periodontal ligament tissue which is related to the osteogenic capacity, whereas the DEC1 expression increases in the suprabasal layer of gingival epithelial cells which are involved in immune inflammatory response in the patients with chronic periodontitis. The findings provide a new target to explore the pathology and the therapy of periodontitis.
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http://dx.doi.org/10.1016/j.archoralbio.2014.12.006DOI Listing
April 2015

Down regulation of differentiated embryo-chondrocyte expressed gene 1 is related to the decrease of osteogenic capacity.

Curr Drug Targets 2014 Apr;15(4):432-41

Department of pharmacology, Nanjing medicine University, Nanjing, 210029, China.

Differentiated embryo-chondrocyte expressed gene 1 (DEC1) is associated with chondrogenesis, neurogenesis, immune response, biological rhythm, lipogenesis, cell differentiation and carcinogenesis. However, there is little information about its contribution to osteoblast osteogenesis. In the present study, we report that DEC1 expression increases along with the degree of mineralization, which parallells with the increase of osteogenesis induction time in SaoS-2 cells. Dexamethasone (DEX) decreases the osteogenesis capacity such as alkaline phosphatase (ALP) activity and mineralization along with decreasing the DEC1 expression. On the contrary, 17β-estradiol (E2) increases the osteogenesis along with increasing the DEC1 expression. Moreover, the overexpression of DEC1 alone increases the ALP activity and mineralization synchronously, and it not only partially reverses the decrease of ALP activity induced by DEX, but almost abolishes the decrease of mineralized nodules induced by DEX. On the other hand, the DEC1 expression decreases in tibia bone marrow side of ovariectomy mice compared with that in sham-operated mice, and E2 treatment ameliorates the decrease of DEC1 expression induced by bilateral ovariectomy and prevents osteoporosis in ovariectomized mice . Taken together, downregulation of DEC1 expression is related to the decrease of osteogenic capacity. The findings provide a novel target for the therapy of osteoporosis.
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http://dx.doi.org/10.2174/1389450114666140102133719DOI Listing
April 2014

Curcumin Protects against 1-Methyl-4-phenylpyridinium Ion- and Lipopolysaccharide-Induced Cytotoxicities in the Mouse Mesencephalic Astrocyte via Inhibiting the Cytochrome P450 2E1.

Evid Based Complement Alternat Med 2013 17;2013:523484. Epub 2013 Jun 17.

Department of Pharmacology, Nanjing Medical University, 140 Hanzhong Road, Jiangsu, Nanjing 210029, China.

Curcumin is extracted from the rhizomes of the ginger family plant Curcuma longa L., which has a good protection for liver, kidney, and immune system. However, there is little information about its contribution in protection of astrocytes recently. The present study was undertaken to elucidate the protective effect of curcumin, an herbal antioxidant, on 1-methyl-4-phenylpyridinium ion- (MPP(+)-) and lipopolysaccharide- (LPS-) induced cytotoxicities, as well as the underlying mechanisms by using primary mouse mesencephalic astrocytes. The results showed that curcumin protected the mesencephalic astrocytes from MPP(+)- and LPS-induced toxicities along with reducing reactive oxygen species (P < 0.05) and maleic dialdehyde (P < 0.05) sufficiently. Moreover, curcumin significantly inhibited the cytochrome P450 2E1 (CYP2E1) expression (P < 0.01 at mRNA level, P < 0.05 at protein level) and its activity (P < 0.05) sufficiently induced by MPP(+) and LPS in the mouse mesencephalic astrocytes. And curcumin as well as diallyl sulphide, a CYP2E1 positive inhibitor, ameliorated MPP(+)- and LPS-induced mouse mesencephalic astrocytes damage. Accordingly, curcumin protects against MPP(+)- and LPS-induced cytotoxicities in the mouse mesencephalic astrocyte via inhibiting the CYP2E1 expression and activity.
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http://dx.doi.org/10.1155/2013/523484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703411PMC
July 2013

[Influence of smoking on survival rate of endosseous implant: a meta analysis].

Shanghai Kou Qiang Yi Xue 2013 Feb;22(1):89-95

Department of Stomatology, Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, China.

Purpose: To evaluate the influence of smoking on survival rate of endosseous implant.

Methods: The literatures published before the year 2012 involving influence of smoking on survival rate of endosseous implant were searched in PubMed,Embase and Cochrane databases. Data from the literatures conforming to the inclusion criteria and exclusive criteria were selected and a meta-analysis was carried out.

Results: Sixteen literatures conforming to the inclusion criteria and exclusive criteria were screened out, which were prospective or retrospective cohort studies. Meta analysis using random effects model showed smoking patients had higher risk to lose endosseous implants than non-smoking patients (synthesized OR 2.24,95% CI 1.66-3.01,P<0.01), which implied the survival rate of endosseous implant in smoking patients was lower than in non-smoking patients. Heterogeneity source was not figured out by meta regression and subgroup analysis. When sensitivity analysis was done by using fixed and random effects model alternatively or excluding articles in which the distribution of implant surface characteristics were uneven between smoking and non-smoking group, result was not changed obviously.

Conclusions: The existing limited evidences show that smoking is harmful to the survival of endosseous implants. This meta-analysis is restricted by the literatures' quality and quantity and the results need to be confirmed by high quality studies.
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February 2013

DEC1 binding to the proximal promoter of CYP3A4 ascribes to the downregulation of CYP3A4 expression by IL-6 in primary human hepatocytes.

Biochem Pharmacol 2012 Sep 21;84(5):701-711. Epub 2012 Jun 21.

Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, Rhode Island, 02881, USA.

In this study, we provided molecular evidences that interleukin-6 (IL-6) contributed to the decreased capacity of oxidative biotransformation in human liver by suppressing the expression of cytochrome P450 3A4 (CYP3A4). After human hepatocytes were treated with IL-6, differentially expressed in chondrocytes 1 (DEC1) expression rapidly increased, and subsequently, the CYP3A4 expression decreased continuously. Furthermore, the repression of CYP3A4 by IL-6 occurred after the increase of DEC1 in primary human hepatocytes. In HepG2 cells, knockdown of DEC1 increased the CYP3A4 expression and its enzymatic activity. In addition, it partially abolished the decreased CYP3A4 expression as well as its enzymatic activity induced by IL-6. Consistent with this, overexpression of DEC1 markedly reduced the CYP3A4 promoter activity and the CYP3A4 expression as well as its enzymatic activity. Using sequential truncation and site directed mutagenesis of CYP3A4 proximal promoter with DEC1 construct, we showed that DEC1 specifically bound to CCCTGC sequence in the proximal promoter of CYP3A4, which was validated by EMSA and ChIP assay. These findings suggest that the repression of CYP3A4 by IL-6 is achieved through increasing the DEC1 expression in human hepatocytes, the increased DEC1 binds to the CCCTGC sequence in the promoter of CYP3A4 to form CCCTGC-DEC1 complex, and the complex downregulates the CYP3A4 expression and its enzymatic activity.
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http://dx.doi.org/10.1016/j.bcp.2012.06.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4088276PMC
September 2012

Lipopolysaccharide down-regulates carbolesterases 1 and 2 and reduces hydrolysis activity in vitro and in vivo via p38MAPK-NF-κB pathway.

Toxicol Lett 2011 Mar 13;201(3):213-20. Epub 2011 Jan 13.

Department of Pharmacology, Nanjing Medical University, 140 Han Zhong Rd., Nanjing, Jiangsu 210029, China.

Carboxylesterases constitute a class of enzymes that hydrolyze drugs containing such functional groups as carboxylic acid ester, amide, and thioester. Hydrolysis of many drugs is reduced in liver diseases such as hepatitis and cirrhosis. In this study, we have demonstrated, in vitro and in vivo, treatment with LPS decreased the expression of HCE1 and HCE2 and the capacity of hydrolytic activity. In HepG2 cells, the decreased expression by LPS occurred at both mRNA and protein levels. Both HCE1 and HCE2 promoters were significantly repressed by LPS, and the repression was comparable with the decrease in HCE1 and HCE2 mRNA, suggesting the transrepression is responsible for suppressed expression. Further study showed that both PDTC, a NF-κB inhibitor, and SB203580, a p38MAPK inhibitor, could abolish the repression of HCE1 and HCE2 mediated by LPS, but U0126, a selective ERK1/2 inhibitor, could not do so, suggesting the repression of HCE1 and HCE2 by LPS through the p38MAPK-NF-κB pathway. In addition, being pretreated with LPS, HepG2 cells altered the cellular responsiveness to ester therapeutic agents, including clopidogrel (hydrolyzed by HCE1) and irinotecan (hydrolyzed by HCE2). The altered cellular responsiveness occurred at low micromolar concentrations, suggesting that suppressed expression of carboxylesterases by LPS has profound pharmacological and toxicological consequences, particularly with those that are hydrolyzed in an isoform-specific manner. This study provides new insight into the understanding of the pharmacological and toxicological effects and the mechanisms for repressing drug metabolism enzymes in inflammation.
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http://dx.doi.org/10.1016/j.toxlet.2011.01.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284319PMC
March 2011

[Allergy caused by minidose and low concentration Pingyangmycin: a case report].

Hua Xi Kou Qiang Yi Xue Za Zhi 2009 Oct;27(5):572-3

Dept. of Stomatology, Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, China.

The emergence of allergy caused by Pingyangmycin is rare. A case of allergy caused by minidose and low concentration Pingyangmycin was reported in this article.
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October 2009

[Study on friction and abrasion behavior of TiN film on dental NiCr alloy by plasma magnetron reactive sputter deposition].

Shanghai Kou Qiang Yi Xue 2008 Dec;17(6):629-32

Department of Stomatology, Nanjing General Hospital of Nanjing Command, Nanjing 210002, China.

Purpose: To investigate the effect of TiN film deposited by plasma magnetron reactive sputter deposition technique on the fiction and abrasion behavior of dental NiCr alloy.

Methods: TiN film was deposited on the surface of dental NiCr alloy by plasma magnetron reactive sputter deposition technique. Surface topography of TiN film was observed by electron microscope. The frictional coefficient and abrasive loss in weight were measured by friction and abrasive apparatus. SPSS11.0 software package was used for Student's t test.

Results: The surface topography of TiN film was tiny diffusion and homogeneous distribution common burreed tuber structure. The average frictional coefficient of NiCr of none deposition was 0.651 while 0.525 after TiN deposited (P<0.05). The average abrasive loss in weight of none deposition was 0.0113g while 0.0007g after TiN deposited (P<0.05).

Conclusions: Compared with non deposition NiCr, the fiction and abrasion behavior of NiCr with TiN film deposited by plasma magnetron reactive sputter deposition technique is better.
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December 2008

[Initial identification of quantitative trait loci controlling resistance to banded leaf and sheath blight at elongating and heading date in maize].

Fen Zi Xi Bao Sheng Wu Xue Bao 2006 Apr;39(2):139-44

The Maize Research Institute, Ministry of Education, Sichuan Agriculture University, Ya'an.

The genetic linkage map was constructed with 146 SSR markers based on a maize population consisting of 229 F2 individuals from the cross R15 (resistant) x 478 (susceptible), covering 1666cM on a total of ten chromosomes, with an average interval length of 11.4 cM. The disease index from the population of 229 F24 lines were evaluated for BLSB resistance under artificial inoculation at elongating stage and heading date. With the method of composite interval mapping (CIM) described in QTL cartographer v2.0 procedure, 9 QTLs among of 17 QTLs were identified on 1, 2, 3, 4, 5, 6 and 10 chromosomes at Jointing Stage, accounting for 3.72% to 9.26% of the phenotypic variance. The other 10 QTLs were identified on 2, 3, 4, 5, 6, 8 and 9 chromosomes at elongating stage and heading date, accounting for 4.27% to 9.27% of the phenotypic variance. Among of them, two QTLs were detected at two stages, which were located between bnlgl600-umc1818 and umc1006-umc1723 on chromosome 2 and 6, respectively. The result indicated that the significant difference about QTL Controlling Resistance between the two stages had a close connection with Developing Stages, which was showed on the resistance of banded leaf and sheath blight in maize. So this result provided new information to the resistance breeding of maize.
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April 2006

The development and identification of constructing tissue engineered bone by seeding osteoblasts from differentiated rat marrow stromal stem cells onto three-dimensional porous nano-hydroxylapatite bone matrix in vitro.

Tissue Cell 2005 Oct;37(5):349-57

Department of Biological Science and Technology and State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China.

The purposes of this study were to develop a new cultural method for the rat bone marrow stromal cells (MSCs) to differentiate into osteoblasts well in vitro, and to investigate the feasibility of using MSCs as seed cells and three-dimensional porous nano-hydroxylapatite as scaffolds for constructing tissue-engineered bone. MSCs of rats were isolated, cultured, induced to differentiate into osteoblasts, and then observed with inverted microscopy. Histochemical staining and radio-immunological analysis were applied for identifying MSCs. Whereafter MSCs were seeded onto three-dimensional porous nano-hydroxylapatite scaffolds, and scanning electron microscopy was applied to evaluate their growth on scaffolds. Results showed that MSCs were typical fibroblast-like and possessed a better proliferating capability; the activity of alkaline phosphatase (ALP) and the secretion of osteocalcin of MSCs were produced gradually and increased continuously; the cells seeded on three-dimensional porous nano-hydroxylapatite scaffolds adhered, proliferated and differentiated well. These results demonstrated that the new improved culture method had the advantages of short isolating time, less risk of contamination and higher efficiency and accordingly was conducive to MSCs proliferating and differentiating into osteoblasts, and that it was advantageous to constructing tissue-engineered bone using MSCs as seed cells and three-dimensional porous nano-hydroxylapatite as scaffolds.
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http://dx.doi.org/10.1016/j.tice.2005.05.003DOI Listing
October 2005
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