Publications by authors named "Mao Yang"

166 Publications

Structure-Composition-Property Relationships in Antiperovskite Nitrides: Guiding a Rational Alloy Design.

ACS Appl Mater Interfaces 2021 Oct 6;13(41):48516-48524. Epub 2021 Oct 6.

School of Physics and Technology, Wuhan University, Wuhan 430072, China.

The alloy strategy through the A- or X-site is a common method for experimental preparation of high-performance and stable lead-based perovskite solar cells. As one of the important candidates for lead-free and stable photovoltaic absorbers, the inorganic antiperovskite family has recently been reported to exhibit excellent optoelectronic properties. However, the current reports on the design of antiperovskite alloys are rare. In this work, we investigated the previously overlooked electronic property (e.g., conduction band convergence), static dielectric constant, and exciton binding energy in inorganic antiperovskite nitrides by first-principles calculations. Then, we revealed a linear relationship between the tolerance factor and various physical quantities. Guided by the established structure-composition-property relationship in six antiperovskite nitrides XNA (X = Mg, Ca, Sr; A = P, As, Sb, Bi), for the first time, we designed a promising antiperovskite alloy MgNAsBi with a quasi-direct band gap of 1.402 eV. Finally, we made a comprehensive comparison between antiperovskite nitrides and conventional halide perovskites for pointing out the future direction for device applications.
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http://dx.doi.org/10.1021/acsami.1c10137DOI Listing
October 2021

Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L.

BMC Plant Biol 2021 Aug 5;21(1):359. Epub 2021 Aug 5.

National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, 210095, China.

Background: Plant height is an important architecture trait which is a fundamental yield-determining trait in crops. Variety with dwarf or semi-dwarf phenotype is a major objective in the breeding because dwarfing architecture can help to increase harvest index, increase planting density, enhance lodging resistance, and thus be suitable for mechanization harvest. Although some germplasm or genes associated with dwarfing plant type have been carried out. The molecular mechanisms underlying dwarfism in oilseed rape (Brassica napus L.) are poorly understood, restricting the progress of breeding dwarf varieties in this species. Here, we report a new dwarf mutant Bndwarf2 from our B. napus germplasm. We studied its inheritance and mapped the dwarf locus BnDWARF2.

Results: The inheritance analysis showed that the dwarfism phenotype was controlled by one semi-dominant gene, which was mapped in an interval of 787.88 kb on the C04 chromosome of B. napus by Illumina Brassica 60 K Bead Chip Array. To fine-map BnDWARF2, 318 simple sequence repeat (SSR) primers were designed to uniformly cover the mapping interval. Among them, 15 polymorphic primers that narrowed down the BnDWARF2 locus to 34.62 kb were detected using a F family population with 889 individuals. Protein sequence analysis showed that only BnaC04.BIL1 (BnaC04g41660D) had two amino acid residues substitutions (Thr187Ser and Gln399His) between ZS11 and Bndwarf2, which encoding a GLYCOGEN SYNTHASE KINASE 3 (GSK3-like). The quantitative real-time PCR (qRT-PCR) analysis showed that the BnaC04.BIL1 gene expressed in all tissues of oilseed rape. Subcellular localization experiment showed that BnaC04.BIL1 was localized in the nucleus in tobacco leaf cells. Genetic transformation experiments confirmed that the BnaC04.BIL1 is responsible for the plant dwarf phenotype in the Bndwarf2 mutants. Overexpression of BnaC04.BIL1 reduced plant height, but also resulted in compact plant architecture.

Conclusions: A dominant dwarfing gene, BnaC04.BIL1, encodes an GSK3-like that negatively regulates plant height, was mapped and isolated. Our identification of a distinct gene locus may help to improve lodging resistance in oilseed rape.
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http://dx.doi.org/10.1186/s12870-021-03137-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340546PMC
August 2021

Systematic Evaluation of Fragmentation Methods for Unlabeled and Isobaric Mass Tag-Labeled O-Glycopeptides.

Anal Chem 2021 08 4;93(32):11167-11175. Epub 2021 Aug 4.

Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen 2200, Denmark.

Dissecting site-specific functions of O-glycosylation requires simultaneous identification and quantification of differentially expressed O-glycopeptides by mass spectrometry. However, different dissociation methods have not been systematically compared in their performance in terms of identification, glycosite localization, and quantification with isobaric labeling. Here, we conducted this comparison on highly enriched unlabeled O-glycopeptides with higher-energy collision dissociation (HCD), electron-transfer/collision-induced dissociation (ETciD), and electron transfer/higher-energy collisional dissociation (EThcD), concluding that ETciD and EThcD with optimal supplemental activation resulted in superior identification of glycopeptides and unambiguous site localizations than HCD in a database search by Sequest HT. We later described a pseudo-EThcD strategy that concatenates the electron transfer dissociation spectrum with the paired HCD spectrum acquired sequentially for the same precursor ions, which combines the identification advantage of ETciD/EThcD with the superior reporter ion quality of HCD. We demonstrated its improvements in identification and quantification of isobaric mass tag-labeled O-glycopeptides and showcased the discovery of the specific glycosites of GalNAc transferase 11 (GALNT11) in HepG2 cells.
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http://dx.doi.org/10.1021/acs.analchem.1c01696DOI Listing
August 2021

Molecular Evolution of clock Genes in Vertebrates.

J Mol Evol 2021 08 23;89(7):494-512. Epub 2021 Jul 23.

Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Sciences, Chongqing, 400715, China.

Circadian rhythms not only influence the overall daily routine of organisms but also directly affect life activities to varying degrees. Circadian locomotor output cycle kaput (Clock), the most critical gene in the circadian rhythm feedback system, plays an important role in the regulation of biological rhythms. Here, we aimed to elucidate the evolutionary history of the clock gene family in a taxonomically diverse set of vertebrates, providing novel insights into the evolution of the clock gene family based on 102 vertebrate genomes. Using genome-wide analysis, we extracted 264 clock sequences. In lobe-finned fishes and some basal non-teleost ray-finned fishes, only two clock isotypes were found (clock1 and clock2). However, the majority of teleosts possess three clock genes (two clock1 genes and one clock2 gene) owing to extra whole-genome duplication. The following syntenic analysis confirmed that clock1a, clock1b, and clock2 are conserved in teleost species. Interestingly, we discovered that osteoglossomorph fishes possess two clock2 genes. Moreover, protein sequence comparisons indicate that CLOCK protein changes among vertebrates were concentrated at the N-terminal and poly Q regions. We also performed a dN/dS analysis, and the results suggest that clock1 and clock2 may show distinct fates for duplicated genes between the lobe-finned and ray-finned fish clades. Collectively, these results provide a genome-wide insight into clock gene evolution in vertebrates.
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http://dx.doi.org/10.1007/s00239-021-10020-6DOI Listing
August 2021

Effects of different biomass materials as a salt-isolation layer on water and salt migration in coastal saline soil.

PeerJ 2021 7;9:e11766. Epub 2021 Jul 7.

School of Resources and Environmental Engineering, Ludong University, Yantai, Shandong, China.

The aim of this study was to find a material suited for the prevention of evaporative water loss and salt accumulation in coastal saline soils. One-dimensional vertical water infiltration and phreatic evaporation experiments were conducted using a silty loam saline soil. A 3-cm-thick layer of corn straw, biochar, and peat was buried at the soil depth of 20 cm, and a 6-cm-thick layer of peat was also buried at the same soil depth for comparison. The presence of the biochar layer increased the upper soil water content, but its ability to inhibit salt accumulation was poor, leading to a high salt concentration in the surface soil. The 3-cm-thick straw and 6-cm-thick peat layers were most effective to inhibit salt accumulation, which reduced the upper soil salt concentration by 96% and 93%, respectively. However, the straw layer strongly inhibited phreatic evaporation and resulted in low water content in the upper soil layer. Compared with the straw layer, the peat layer increased the upper soil water content. Thus, burying a 6-cm-thick peat layer in the coastal saline soil is the optimal strategy to retain water in the upper soil layer and intercept salt in the deeper soil layer.
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http://dx.doi.org/10.7717/peerj.11766DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272462PMC
July 2021

A Novel System for Simple Rapid Adenoviral Vector Construction to Facilitate CRISPR/Cas9-Mediated Genome Editing.

CRISPR J 2021 06 2;4(3):381-391. Epub 2021 Jun 2.

Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Recombinant adenoviruses have broad applications for gene delivery and expression. Furthermore, the adenovirus packaging system facilitates the expression of RNA-guided CRISPR/Cas9 nuclease complexes. In this study, we developed a novel system, named AdBlue, for the construction of recombinant adenoviruses using an enzymatic assembly strategy. This system could significantly reduce the time and labor required to generate adenoviral vectors. When applied to CRISPR/Cas9 design, it simplifies the preparation of recombinant adenoviruses carrying nuclease complexes and can induce high levels of site-specific mutagenesis. Our system has outstanding advantages for adenovirus preparation and could be a useful molecular engineering tool for gene delivery and editing.
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http://dx.doi.org/10.1089/crispr.2020.0110DOI Listing
June 2021

ERp44/CG9911 promotes fat storage in adipocytes by regulating ER Ca homeostasis.

Aging (Albany NY) 2021 05 24;13(11):15013-15031. Epub 2021 May 24.

Key Laboratory of Interdisciplinary Research, Chinese Academy of Sciences, Beijing 100101, China.

Fat storage is one of the important strategies employed in regulating energy homeostasis. Impaired lipid storage causes metabolic disorders in both mammals and . In this study, we report CG9911, the homolog of ERp44 (endoplasmic reticulum protein 44) plays a role in regulating adipose tissue fat storage. Using the CRISPR/Cas9 system, we generated a CG9911 mutant line deleting 5 bp of the coding sequence. The mutant flies exhibit phenotypes of lower bodyweight, fewer lipid droplets, reduced TAG level and increased expression of lipolysis related genes. The increased lipolysis phenotype is enhanced in the presence of ER stresses and suppressed by a reduction of the ER Ca. Moreover, loss of results in a decrease of ER Ca in the fat body. Together, our results reveal a novel function of CG9911 in promoting fat storage via regulating ER Ca signal in .
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http://dx.doi.org/10.18632/aging.203063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221293PMC
May 2021

Improving Cyclability of Lithium Metal Anode via Constructing Atomic Interlamellar Ion Channel for Lithium Sulfur Battery.

Nanoscale Res Lett 2021 Mar 23;16(1):52. Epub 2021 Mar 23.

State Key Laboratory of Electronic Thin Films and Integrated Devices, University of Electronic Science and Technology of China, Chengdu, 610054, China.

Uniform migration of lithium (Li) ions between the separator and the lithium anode is critical for achieving good quality Li deposition, which is of much significance for lithium metal battery operation, especially for Li-sulfur (Li-S) batteries. Commercial separators such as polypropylene or polyethylene can be prepared by wet or dry processes, but they can indeed cause plentiful porosities, resulting in the uneven Li ion stripping/plating and finally the formation of Li dendrites. Thence, we constructed an atomic interlamellar ion channel by introducing the layered montmorillonite on the surface of the separator to guide Li ion flux and achieved stable Li deposition. The atomic interlamellar ion channel with a spacing of 1.4 nm showed strong absorption capacity for electrolytes and reserved capacity for Li ions, thus promoting rapid transfer of Li ions and resulting in even Li ion deposition at the anode. When assembled with the proposed separator, the Coulombic efficiency of Li||Cu batteries was 98.2% after 200 cycles and stable plating/stripping even after 800 h was achieved for the Li||Li symmetric batteries. Importantly, the proposed separator allows 140% specific capacity increase after 190 cycles as employing the Li-S batteries.
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http://dx.doi.org/10.1186/s11671-021-03508-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988023PMC
March 2021

Knockout of the Gene Inhibits Neointima Formation in a Mouse Model of Vascular Injury.

Arterioscler Thromb Vasc Biol 2021 04 25;41(4):1428-1445. Epub 2021 Feb 25.

Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China (Xingli Xu, Xinghua Xu, Y.M., L.L., J.M., T.Z., J.Z., M.Z., L. Meng, L. Ma, J.C., W.C., H.J., Y.Z., C.Z.).

[Figure: see text].
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http://dx.doi.org/10.1161/ATVBAHA.119.313581DOI Listing
April 2021

Association between Baseline SBP/DBP and All-Cause Mortality in Residents of Shanxi, China: A Population-based Cohort Study from 2002 to 2015.

Biomed Environ Sci 2021 Jan;34(1):1-8

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: To investigate the association between blood pressure and all-cause mortality in Shanxi, China.

Methods: The '2002 China Nutrition and Health Survey' baseline data in Shanxi province was used. A retrospective investigation was performed in 2015. The effects of SBP and DBP on the all-cause mortality were analyzed using the Cox regression model. The hazard ratio ( ) and 95% confidence interval ( ) were estimated by the sex and age groups.

Results: The follow-up rate was 76.52% over 13 years, while the cumulative mortality rate for all participants was 917.12/100,000 person-years. The mortality rose with an increasing SBP ( = 270.537, < 0.001) or DBP level ( = 57.240, < 0.001). After adjustment for the confounding factors, a significant association between mortality and high SBP (≥ 160 mmHg) and high DBP (≥ 100 mmHg), with adjusted ranging from 1.405- to 2.179-fold for SBP and 1.550- to 2.854-fold for DBP, was noted. Significant HRs for most DBP subgroups were found in > 60-year-old participants. Males with DBP ≥ 100 mmHg had a significantly higher mortality, with an (95% ) of 2.715 (1.377-5.351).

Conclusion: Adults with SBP > 160 mmHg and DBP > 100 mmHg had a higher mortality risk. Sex and age difference was noted in both DBP and mortality risk.
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http://dx.doi.org/10.3967/bes2021.002DOI Listing
January 2021

High-mechanical strength carboxymethyl chitosan-based hydrogel film for antibacterial wound dressing.

Carbohydr Polym 2021 Mar 4;256:117590. Epub 2021 Jan 4.

Institute for Biomass and Function Materials & National Demonstration Centre for Experimental Light Chemistry Engineering Education, College of Bioresources Chemistry and Materials Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China. Electronic address:

Hydrogels, being highly biocompatible and adaptable with biological tissues, have shown great usability in biomedical applications. In this research, a novel hydrogel film developed from carboxymethyl chitosan (CMCS) loaded with waterborne polyurethane-gelatin hydrolysate was synthesized via aqueous emulsion copolymerization. The synthesized hydrogel film was characterized using mechanical strength tests, FTIR, XPS, SEM, AFM, and various other analysis technologies. The results demonstrated that the hydrogel film exhibited good thermal stability, swelling behavior, as well as controllable biodegradability. Specifically, when the CMCS content was loaded at 6 %, the maximum tensile strength and elongation at the break of the hydrogel film were reached 31.69 MPa and 447.187, respectively. The disk diffusion tests indicated that the hydrogel film presented significant antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). These results indicate that hydrogel films with high mechanical strength and high antibacterial activity could be used for wound dressing applications.
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http://dx.doi.org/10.1016/j.carbpol.2020.117590DOI Listing
March 2021

Asthma and its relationship to mitochondrial copy number: Results from the Asthma Translational Genomics Collaborative (ATGC) of the Trans-Omics for Precision Medicine (TOPMed) program.

PLoS One 2020 25;15(11):e0242364. Epub 2020 Nov 25.

Center for Individualized and Genomic Medicine Research (CIGMA), Department of Internal Medicine, Henry Ford Health System, Detroit, Michigan, United States of America.

Background: Mitochondria support critical cellular functions, such as energy production through oxidative phosphorylation, regulation of reactive oxygen species, apoptosis, and calcium homeostasis.

Objective: Given the heightened level of cellular activity in patients with asthma, we sought to determine whether mitochondrial DNA (mtDNA) copy number measured in peripheral blood differed between individuals with and without asthma.

Methods: Whole genome sequence data was generated as part of the Trans-Omics for Precision Medicine (TOPMed) Program on participants from the Study of Asthma Phenotypes and Pharmacogenomic Interactions by Race-ethnicity (SAPPHIRE) and the Study of African Americans, Asthma, Genes, & Environment II (SAGE II). We restricted our analysis to individuals who self-identified as African American (3,651 asthma cases and 1,344 controls). Mitochondrial copy number was estimated using the sequencing read depth ratio for the mitochondrial and nuclear genomes. Respiratory complex expression was assessed using RNA-sequencing.

Results: Average mitochondrial copy number was significantly higher among individuals with asthma when compared with controls (SAPPHIRE: 218.60 vs. 200.47, P<0.001; SAGE II: 235.99 vs. 223.07, P<0.001). Asthma status was significantly associated with mitochondrial copy number after accounting for potential explanatory variables, such as participant age, sex, leukocyte counts, and mitochondrial haplogroup. Despite the consistent relationship between asthma status and mitochondrial copy number, the latter was not associated with time-to-exacerbation or patient-reported asthma control. Mitochondrial respiratory complex gene expression was disproportionately lower in individuals with asthma when compared with individuals without asthma and other protein-encoding genes.

Conclusions: We observed a robust association between asthma and higher mitochondrial copy number. Asthma having an effect on mitochondria function was also supported by lower respiratory complex gene expression in this group.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0242364PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688161PMC
January 2021

Mapping the 17q12-21.1 Locus for Variants Associated with Early-Onset Asthma in African Americans.

Am J Respir Crit Care Med 2021 02;203(4):424-436

Department of Internal Medicine, Center for Individualized and Genomic Medicine Research and.

The 17q12-21.1 locus is one of the most highly replicated genetic associations with asthma. Individuals of African descent have lower linkage disequilibrium in this region, which could facilitate identifying causal variants. To identify functional variants at 17q12-21.1 associated with early-onset asthma among African American individuals. We evaluated African American participants from SAPPHIRE (Study of Asthma Phenotypes and Pharmacogenomic Interactions by Race-Ethnicity) ( = 1,940), SAGE II (Study of African Americans, Asthma, Genes and Environment) ( = 885), and GCPD-A (Study of the Genetic Causes of Complex Pediatric Disorders-Asthma) ( = 2,805). Associations with asthma onset at ages under 5 years were meta-analyzed across cohorts. The lead signal was reevaluated considering haplotypes informed by genetic ancestry (i.e., African vs. European). Both an expression-quantitative trait locus analysis and a phenome-wide association study were performed on the lead variant. The meta-analyzed results from SAPPHIRE, SAGE II, and the GCPD-A identified rs11078928 as the top association for early-onset asthma. A haplotype analysis suggested that the asthma association partitioned most closely with the rs11078928 genotype. Genetic ancestry did not appear to influence the effect of this variant. In the expression-quantitative trait locus analysis, rs11078928 was related to alternative splicing of (gasdermin-B) transcripts. The phenome-wide association study of rs11078928 suggested that this variant was predominantly associated with asthma and asthma-associated symptoms. A splice-acceptor polymorphism appears to be a causal variant for asthma at the 17q12-21.1 locus. This variant appears to have the same magnitude of effect in individuals of African and European descent.
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http://dx.doi.org/10.1164/rccm.202006-2623OCDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885840PMC
February 2021

Astilbin-induced inhibition of the PI3K/AKT signaling pathway decelerates the progression of osteoarthritis.

Exp Ther Med 2020 Oct 27;20(4):3078-3083. Epub 2020 Jul 27.

Miao Medicine Department, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550001, P.R. China.

Degeneration and destruction of articular cartilage are the key characteristics of osteoarthritis (OA). In recent studies, the use of astilbin (AST), the primary active ingredient of , has been shown to correlate with a reduction in inflammatory disease symptoms. The present study aimed to investigate the effects and mechanisms of AST on OA. A rat model of OA was constructed and experiments were performed using the AST, PBS, OA and control groups. The cartilage tissues of each group were assessed by hematoxylin and eosin and toluidine blue staining. The gene expression levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6, AKT, PI3K and other related proteins were analyzed by reverse transcription-quantitative PCR and western blot analysis. AST was found to significantly inhibit IL-1β and TNF-α protein expression; this further confirmed that IL-1β, TNF-α and PI3K mRNA expression was downregulated, indicating that the protective mechanism of AST is associated with the PI3K/AKT pathway. Overall, the results of the present study demonstrate that AST can improve OA symptoms by downregulating the PI3K/AKT signaling pathway, and may therefore be a potential therapeutic option for patients with OA.
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http://dx.doi.org/10.3892/etm.2020.9048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444333PMC
October 2020

Chromosome-level genome assembly of the female western mosquitofish (Gambusia affinis).

Gigascience 2020 08;9(8)

Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Sciences, No. 2 Tiansheng Road, Beibei, Chongqing 400715, China.

Background: The western mosquitofish (Gambusia affinis) is a sexually dimorphic poeciliid fish known for its worldwide biological invasion and therefore an important research model for studying invasion biology. This organism may also be used as a suitable model to explore sex chromosome evolution and reproductive development in terms of differentiation of ZW sex chromosomes, ovoviviparity, and specialization of reproductive organs. However, there is a lack of high-quality genomic data for the female G. affinis; hence, this study aimed to generate a chromosome-level genome assembly for it.

Results: The chromosome-level genome assembly was constructed using Oxford nanopore sequencing, BioNano, and Hi-C technology. G. affinis genomic DNA sequences containing 217 contigs with an N50 length of 12.9 Mb and 125 scaffolds with an N50 length of 26.5 Mb were obtained by Oxford nanopore and BioNano, respectively, and the 113 scaffolds (90.4% of scaffolds containing 97.9% nucleotide bases) were assembled into 24 chromosomes (pseudo-chromosomes) by Hi-C. The Z and W chromosomes of G. affinis were identified by comparative genomic analysis of female and male G. affinis, and the mechanism of differentiation of the Z and W chromosomes was explored. Combined with transcriptome data from 6 tissues, a total of 23,997 protein-coding genes were predicted and 23,737 (98.9%) genes were functionally annotated.

Conclusions: The high-quality female G. affinis reference genome provides a valuable omics resource for future studies of comparative genomics and functional genomics to explore the evolution of Z and W chromosomes and the reproductive developmental biology of G. affinis.
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http://dx.doi.org/10.1093/gigascience/giaa092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450667PMC
August 2020

Fine mapping of the BnUC2 locus related to leaf up-curling and plant semi-dwarfing in Brassica napus.

BMC Genomics 2020 Jul 31;21(1):530. Epub 2020 Jul 31.

National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, 210095, China.

Background: Studies of leaf shape development and plant stature have made important contributions to the fields of plant breeding and developmental biology. The optimization of leaf morphology and plant height to improve lodging resistance and photosynthetic efficiency, increase planting density and yield, and facilitate mechanized harvesting is a desirable goal in Brassica napus.

Results: Here, we investigated a B. napus germplasm resource exhibiting up-curled leaves and a semi-dwarf stature. In progeny populations derived from NJAU5737 and Zhongshuang 11 (ZS11), we found that the up-curled leaf trait was controlled by a dominant locus, BnUC2. We then fine mapped the BnUC2 locus onto an 83.19-kb interval on chromosome A05 using single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) markers. We further determined that BnUC2 was a major plant height QTL that explained approximately 70% of the phenotypic variation in two BCF family populations derived from NJAU5737 and ZS11. This result implies that BnUC2 was also responsible for the observed semi-dwarf stature. The fine mapping interval of BnUC2 contained five genes, two of which, BnaA05g16700D (BnaA05.IAA2) and BnaA05g16720D, were revealed by comparative sequencing to be mutated in NJAU5737. This result suggests that the candidate gene mutation (BnaA05g16700D, encoding Aux/IAA2 proteins) in the conserved Degron motif GWPPV (P63S) was responsible for the BnUC2 locus. In addition, investigation of agronomic traits in a segregated population indicated that plant height, main inflorescence length, and branching height were significantly reduced by BnUC2, whereas yield was not significantly altered. The determination of the photosynthetic efficiency showed that the BnUC2 locus was beneficial to improve the photosynthetic efficiency. Our findings may provide an effective foundation for plant type breeding in B. napus.

Conclusions: Using SNP and SSR markers, a dominant locus (BnUC2) related to up-curled leaves and semi-dwarf stature in B. napus has been fine mapped onto an 83.19-kb interval of chromosome A05 containing five genes. The BnaA05.IAA2 is inferred to be the candidate gene responsible for the BnUC2 locus.
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http://dx.doi.org/10.1186/s12864-020-06947-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430850PMC
July 2020

[SLFN14 inhibits LINE-1 transposition activity].

Yi Chuan 2020 Jul;42(7):669-679

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.

Long interspersed nuclear element-1 (LINE-1) is the only active autonomous transposon in the human genome. Its transposition frequently induces host genome instability, leading to a variety of genetic diseases, including cancers. The host factors play important roles in inhibiting LINE-1 retrotransposition. As an important component of the immune system, the host factor SLFN14 has antiviral activity. Our laboratory shows that SLFN14 possesses potent inhibitory activity against LINE-1 retrotransposition. To explore the potential mechanism of SLFN14 inhibition, we analyzed its effects on transcription, translation, reverse transcription and insertion in the LINE-1 replication cycle. We confirmed that SLFN14 could suppress the LINE-1 mRNA level by affecting its transcription and degradation, thereby diminishing the protein and cDNA levels of LINE-1, which eventually block the LINE-1 retrotransposition. Further, by mapping the active domains of SLFN14, we found its inhibitory activity on LINE-1 being closely related to its endoribonuclease and ribosome binding domains. These results demonstrate the mechanism of SLFN14 in regulating LINE-1 replication, which further provide new insights for improving the regulation network of host factors for controlling genomic instability caused by LINE-1 replication.
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http://dx.doi.org/10.16288/j.yczz.20-081DOI Listing
July 2020

A Spatial Group-Based Multi-User Full-Duplex OFDMA MAC Protocol for the Next-Generation WLAN.

Sensors (Basel) 2020 Jul 9;20(14). Epub 2020 Jul 9.

School of Electronics and Information, Northwestern Polytechnical University, Xi'an 710072, China.

The Wireless Local Area Network (WLAN) has become a dominant piece of technology to carry wireless traffic for Internet of Things (IoT). The next-generation high-density WLAN scenario is very suitable for the development trend of the industrial wireless sensor network. However, in the high-density deployed WLAN scenarios, the access efficiency is low due to severe collisions, and the interference is diffused due to the scattered locations of the parallel access stations (STAs), which results in low area throughput, i.e., low spatial reuse gain. A spatial group-based multi-user full-duplex orthogonal frequency division multiple access (OFDMA) (GFDO) multiple access control (MAC) protocol is proposed. Firstly, the STAs in the network are divided into several spatial groups according to the neighbor channel sensing ability. Secondly, a two-level buffer state report (BSR) information collection mechanism based on -probability is designed. Initially, intra-group STAs report their BSR information to the group header using low transmission power. After that, group headers report both their BSR information collected from their members and inter-group interference information to the access point (AP). Finally, AP schedules two spatial groups without mutual interference to carry on multi-user full duplex transmission on the subchannels in cascading mode. The closed-form formulas are theoretically derived, including the number of uplink STAs successfully collected by AP, the network throughput and area throughput under saturated traffic. The simulation results show that the theoretical analysis coincide with the simulation results. The system throughput of the GFDO protocol is 16.8% higher than that of EnFD-OMAX protocol.
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http://dx.doi.org/10.3390/s20143826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411805PMC
July 2020

Astilbin influences the progression of osteoarthritis in rats by down-regulation of PGE-2 expression via the NF-κB pathway.

Ann Transl Med 2020 Jun;8(12):766

Department of Miao Medicine, the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, China.

Background: Osteoarthritis (OA) is the most common joint disease, affecting most middle-aged and elderly people. Astilin (AST) is the main active ingredient isolated from the traditional Chinese medicine Astilbe chinensis and has anti-inflammatory and anti-arthritis effects. The purpose of this study was to investigate the effect and mechanism of AST on OA in rats mediated by papain.

Methods: In this study, in vivo experiments were conducted to investigate the protective effect and potential mechanism of Astilbin (AST) when it inhibited the development of osteoarthritis (OA).

Results: A rat model of OA is constructed. Through HE staining, it is found that AST can protect the articular surface and reduce damage. The results of immunohistochemical staining also prove that AST can inhibit the expression of prostaglandin E2 (PGE2) and has an excellent inhibitory effect on inflammatory factors. It is found that AST can significantly inhibit the protein expression of interleukin 1 beta (IL-1β), TNF-α, and NF-κB. Polymerase Chain Reaction (PCR) assay shows that the mRNA of IL-1β, TNF-α, and NF-κB is down-regulated, which also proves that the protective mechanism of AST is related to the NF-κB pathway.

Conclusions: In general, this study proves that AST can be a potential therapy for degenerative joint diseases, including OA.
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http://dx.doi.org/10.21037/atm-20-4485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333102PMC
June 2020

Butyrate mitigates TNF-α-induced attachment of monocytes to endothelial cells.

J Bioenerg Biomembr 2020 08 25;52(4):247-256. Epub 2020 Jun 25.

Department of Cardiology, the Fourth Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang, China.

A major cause of late-life health complications is the cardiovascular disease known as atherosclerosis. The process of atherogenesis is marked by endothelial cell dysfunction, the development of atherogenic lesions, and plaque buildup on the intima of the arterial endothelium. This process is fueled primarily by the adhesion of monocytes to endothelial cells via the actions of the cellular adhesion molecules VCAM-1 and E-selectin. When expressed at their basal levels, these molecules are vital to various cellular processes, but when in a state of overproduction, they drive the progression of atherosclerosis by recruiting monocytes to roll along and adhere to the endothelium. TNF-α is theorized to play a causal role in the development of atherosclerosis, but the exact mechanism remains poorly understood. This cytokine is known to upregulate various factors associated with inflammation and oxidative stress, both of which greatly contribute to endothelial dysfunction and atherogenesis. In the present study, we aimed to elucidate the effect of butyrate on these atherogenic processes. Previously not known to have atheroprotective effects, this natural compound shows promise as a treatment for atherosclerosis. In the present study, we found butyrate to exert various anti-inflammatory and downstream regulatory effects. Namely, butyrate ameliorated the overproduction of adhesion molecules, including VCAM-1 and E-selectin, reduced oxidative stress by reducing the levels of ROS and 4-HNE, and suppressed inflammation via inhibition of MCP-1 and IL-8. Additionally, butyrate rescued the reduced expression of the protective factor KLF2, which was mediated through the ERK5 pathway. Thus, butyrate may serve as a promising treatment against atherosclerosis.
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http://dx.doi.org/10.1007/s10863-020-09841-9DOI Listing
August 2020

Huangqi Guizhi Wuwu Decoction for treating cervical radiculopathy: A systematic review and meta-analysis of randomized controlled trials.

Medicine (Baltimore) 2020 Feb;99(7):e19137

First Affiliated Hospital of Anhui University of Chinese Medicine, Shushan District, Anhui, China.

Background: Huangqi Guizhi Wuwu Decoction (HGWD) is a common prescription for the treatment of cervical radiculopathy (CR). And the effectiveness and safety of HGWD for CR were assessed in this study.

Methods: Seven databases were searched. Randomized controlled trials involving HGWD alone or HGWD combined with conventional treatment were enrolled. The authors in pairs independently assessed the risk of bias and extracted the data.

Results: Eight studies involving 783 participants with CR were included. Meta-analysis revealed that the efficacy of HGWD for CR was significantly superior compared with control treatment (risk ratio = 1.12, 95% confidence interval [CI]:1.06-1.19, Z = 3.71; P = .0002). Compare with control group, there is an increase in visual analog scale (mean difference [MD] = 0.99; 95% CI: 0.83-1.14; Z = 12.57; P < .00001). There was also an improvement of neck disability index (MD = 9.2; 95% CI: 8.28-10.11; Z = 19.75; P < .00001). Adverse events were not mentioned in the 8 trials.

Conclusion: HGWD alone or HGWD plus other treatment may be helpful to patients with CR. However, the methodological quality of the randomized controlled trials was generally low. Larger and better-designed randomized controlled trials are recommended.
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http://dx.doi.org/10.1097/MD.0000000000019137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035008PMC
February 2020

LC-DFSA: Low Complexity Dynamic Frame Slotted Aloha Anti-Collision Algorithm for RFID System.

Sensors (Basel) 2019 Dec 31;20(1). Epub 2019 Dec 31.

School of Electronics and Information, Northwestern Polytechnical University, Xi'an 710072, China.

With the rapid development of the Internet of Things (IoT), the radio frequency identification (RFID) system becomes increasingly important. Tag identification is a basic problem of the RFID system, whose purpose is to inventory tags. However, in recent years, it requires a very short time for massive tag identification, which brings serious challenges. The traditional Aloha based anti-collision algorithms have disadvantages of either low efficiency or high complexity. Therefore, this article proposes a low complexity dynamic frame slotted Aloha (DFSA) anti-collision algorithm, named LC-DFSA. The reader can estimate the range of tag numbers according to the last frame size, the number of successful slots and the ratio of idle slots. Then the optimal frame size can be calculated. Complexity analysis is deployed in this article, and we validate the correctness of the analysis. Through our simulations, LC-DFSA outperforms other schemes in both the average access efficiency and the algorithm complexity. It also can be conveniently applied to engineering implementations.
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http://dx.doi.org/10.3390/s20010228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983178PMC
December 2019

Camera calibration using a planar target with pure translation.

Appl Opt 2019 Nov;58(31):8362-8370

This paper presents a novel camera calibration method using a planar target with pure translation and a known translation distance. It only requires the straightness and position accuracy of the one-axis translational platform. There are theoretically no constraints among the normal direction of the planar target, the optical axis direction of the camera, and the moving direction of the translational platform. The paper analyzes the closed-form solution, followed by a nonlinear refinement based on the maximum likelihood criterion. Both computer simulation and real data are implemented to verify the effectiveness of the proposed method. Compared with Zhang's method, the proposed method realizes the camera calibration process automatically and evaluates the calibration process via the measurement accuracy of the calibrated camera, so it is a key factor to advance 3D computer vision one more step from expert to novice use.
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http://dx.doi.org/10.1364/AO.58.008362DOI Listing
November 2019

Fibroblast growth factor-2/platelet-derived growth factor enhances atherosclerotic plaque stability.

J Cell Mol Med 2020 01 21;24(1):1128-1140. Epub 2019 Nov 21.

Department of Cardiology, The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China.

Increased immature neovessels contribute to plaque growth and instability. Here, we investigated a method to establish functional and stable neovessel networks to increase plaque stability. Rabbits underwent aortic balloon injury and were divided into six groups: sham, vector and lentiviral transfection with vascular endothelial growth factor-A (VEGF)-A, fibroblast growth factor (FGF)-2, platelet-derived growth factor (PDGF)-BB and FGF-2 + PDGF-BB. Lentivirus was percutaneously injected into the media-adventitia of the abdominal aorta by intravascular ultrasound guidance, and plaque-rupture rate, plaque-vulnerability index and plaque neovessel density at the injection site were evaluated. Confocal microscopy, Prussian Blue assay, Evans Blue, immunofluorescence and transmission electron microscopy were used to assess neovessel function and pericyte coverage. To evaluate the effect of FGF-2/PDGF-BB on pericyte migration, we used the mesenchymal progenitor cell line 10T1/2 as an in vitro model. VEGF-A- and FGF-2-overexpression increased the number of immature neovessels, which caused intraplaque haemorrhage and inflammatory cell infiltration, eventually resulting in the plaque vulnerability; however, FGF-2/PDGF-BB induced mature and functional neovessels, through increased neovessel pericyte coverage. Additionally, in vitro analysis of 10T1/2 cells revealed that FGF-2/PDGF-BB induced epsin-2 expression and enhanced the VEGF receptor-2 degradation, which negatively regulated pericyte function consistent with the in vivo data. These results showed that the combination of FGF-2 and PDGF-BB promoted the function and maturation of plaque neovessels, thereby representing a novel potential treatment strategy for vulnerable plaques.
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http://dx.doi.org/10.1111/jcmm.14850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933359PMC
January 2020

Galnt11 regulates kidney function by glycosylating the endocytosis receptor megalin to modulate ligand binding.

Proc Natl Acad Sci U S A 2019 12 18;116(50):25196-25202. Epub 2019 Nov 18.

Developmental Glycobiology Section, National Institute of Dental and Craniofacial Research (NIDCR), National Institutes of Health, Bethesda, MD 20892-4370;

Chronic kidney disease (CKD) affects more than 20 million Americans and ∼10% of the population worldwide. Genome-wide association studies (GWAS) of kidney functional decline have identified genes associated with CKD, but the precise mechanisms by which they influence kidney function remained largely unexplored. Here, we examine the role of 1 GWAS-identified gene by creating mice deficient for , which encodes a member of the enzyme family that initiates protein O-glycosylation, an essential posttranslational modification known to influence protein function and stability. We find that -deficient mice display low-molecular-weight proteinuria and have specific defects in proximal tubule-mediated resorption of vitamin D binding protein, α-microglobulin, and retinol binding protein. Moreover, we identify the endocytic receptor megalin (LRP2) as a direct target of Galnt11 in vivo. Megalin in -deficient mice displays reduced ligand binding and undergoes age-related loss within the kidney. Differential mass spectrometry revealed specific sites of Galnt11-mediated glycosylation within mouse kidney megalin/LRP2 that are known to be involved in ligand binding, suggesting that O-glycosylation directly influences the ability to bind ligands. In support of this, recombinant megalin containing these sites displayed reduced albumin binding in cells deficient for Our results provide insight into the association between and CKD, and identify a role for Galnt11 in proper kidney function.
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http://dx.doi.org/10.1073/pnas.1909573116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911204PMC
December 2019

The effect of exercise on cervical radiculopathy: A systematic review and meta-analysis.

Medicine (Baltimore) 2019 Nov;98(45):e17733

Wangjing Hospital of China Academy of Chinese Medical Sciences, Chaoyang District.

Background: Cervical radiculopathy (CR), which is most often stems from degenerative disease in the cervical spine, has increasingly become a common and frequently occurring disease in clinic due to the popularity of electronic products, such as computes and cell phones. Some studies have shown that exercise or exercise combined with other treatments can effectively decrease pain and improve functional status. The objective was to analyze the effects of exercise for treating patients with CR.

Methods: Seven databases were searched from inception to December 2018. Randomized controlled trials involving exercise alone or exercise combined with conventional treatment were enrolled. Data were pooled after trials quality assessment for meta-analysis. Outcomes were pain (visual analog scale [VAS]), quality of life (12-short form health survey, 36-short form health survey), and physical function accessed by neck disability index (NDI).

Results: Ten studies involving 871 participants with CR were included. Meta-analysis revealed that compared with control group, there was a reduction in VAS (standardized mean difference = -0.89; 95% confidence interval [CI]: -1.34 to -0.44; Z = 3.89; P < .001). There was also an improvement of NDI (mean difference = -3.60; 95% CI: -6.27 to -0.94; Z = 2.65; P = .008)]. Additionally, although the results of subgroup analyses were changed due to the paucity of the quantity and quality of the included studies. The pooled results were verified to be stable by sensitivity analyses. Besides, the grading of recommendations assessment, development, and evaluation level of evidence is low for each outcome.

Conclusion: Exercise alone or exercise plus other treatment may be helpful to patients with CR. However, exercise option should be carefully considered for each patient with CR in accordance with their different situations. Large-scale studies using proper methodology are recommended.
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http://dx.doi.org/10.1097/MD.0000000000017733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6855577PMC
November 2019

Glyco-DIA: a method for quantitative O-glycoproteomics with in silico-boosted glycopeptide libraries.

Nat Methods 2019 09 5;16(9):902-910. Epub 2019 Aug 5.

Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.

We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins. This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification). The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures. Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.
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http://dx.doi.org/10.1038/s41592-019-0504-xDOI Listing
September 2019

Fine mapping of an up-curling leaf locus (BnUC1) in Brassica napus.

BMC Plant Biol 2019 Jul 19;19(1):324. Epub 2019 Jul 19.

National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, 210095, China.

Background: Leaf shape development research is important because leaf shapes such as moderate curling can help to improve light energy utilization efficiency. Leaf growth and development includes initiation of the leaf primordia and polar differentiation of the proximal-distal, adaxial-abaxial, and centrolateral axes. Changes in leaf adaxial-abaxial polarity formation, auxin synthesis and signaling pathways, and development of sclerenchyma and cuticle can cause abnormal leaf shapes such as up-curling leaf. Although many genes related to leaf shape development have been reported, the detailed mechanism of leaf development is still unclear. Here, we report an up-curling leaf mutant plant from our Brassica napus germplasm. We studied its inheritance, mapped the up-curling leaf locus BnUC1, built near-isogenic lines for the Bnuc1 mutant, and evaluated the effect of the dominant leaf curl locus on leaf photosynthetic efficiency and agronomic traits.

Results: The up-curling trait was controlled by one dominant locus in a progeny population derived from NJAU5734 and Zhongshuang 11 (ZS11). This BnUC1 locus was mapped in an interval of 2732.549 kb on the A05 chromosome of B. napus using Illumina Brassica 60 K Bead Chip Array. To fine map BnUC1, we designed 201 simple sequence repeat (SSR) primers covering the mapping interval. Among them, 16 polymorphic primers that narrowed the mapping interval to 54.8 kb were detected using a BCF family population with 654 individuals. We found six annotated genes in the mapping interval using the B. napus reference genome, including BnaA05g18250D and BnaA05g18290D, which bioinformatics and gene expression analyses predicted may be responsible for leaf up-curling. The up-curling leaf trait had negative effects on the agronomic traits of 30 randomly selected individuals from the BCF population. The near-isogenic line of the up-curling leaf (ZS11-UC1) was constructed to evaluate the effect of BnUC1 on photosynthetic efficiency. The results indicated that the up-curling leaf trait locus was beneficial to improve the photosynthetic efficiency.

Conclusions: An up-curling leaf mutant Bnuc1 was controlled by one dominant locus BnUC1. This locus had positive effects on photosynthetic efficiency, negative effects on some agronomic traits, and may help to increase planting density in B. napus.
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http://dx.doi.org/10.1186/s12870-019-1938-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6642557PMC
July 2019

The role of ERp44 in glucose and lipid metabolism.

Arch Biochem Biophys 2019 08 5;671:175-184. Epub 2019 Jul 5.

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China. Electronic address:

Endoplasmic Reticulum Protein 44 (ERp44) is a member of the PDI family, named for a molecular weight of 44 kD. White adipose tissue has metabolic and endocrine functions that are important to metabolism. The role of ERp44 in glucose and lipid metabolism is not known yet. The current study was undertaken to investigate the implication of ERp44 in glucose and lipid metabolism. In this study, we generated and characterized ERp44 mice. We used type 2 diabetes models and ERp44 knockout mice to show the implication of ERp44 in glucose and lipid metabolism. Knockout newborns had lower blood glucose compared to wild-type. Adult knockouts had abnormal intraperitoneal, glucose, insulin and pyruvic acid tolerance. Lipocytes were smaller and fewer in knockout mice compared to wild-type. Knockouts resisted to high-fat diet-induced obesity. ERp44 expression in white adipose tissue decreased significantly in type 2 diabetes models. Results suggest that ERp44 is closely associated with glucose and lipid metabolism.
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http://dx.doi.org/10.1016/j.abb.2019.06.011DOI Listing
August 2019
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