Publications by authors named "Manman Zhang"

68 Publications

Neuroanatomical and functional substrates of the greed personality trait.

Brain Struct Funct 2021 May 8;226(4):1269-1280. Epub 2021 Mar 8.

Key Research Base of Humanities and Social Sciences of the Ministry of Education, Academy of Psychology and Behavior, Tianjin Normal University, Tianjin, 300387, China.

Greedy individuals often exhibit more impulsive decision-making and short-sighted behaviors. It has been assumed that altered reward circuitry and prospection network is associated with greed personality trait (GPT). In this study, we first explored the morphological characteristics (i.e., gray matter volume; GMV) of GPT combined with univariate and multivariate pattern analysis (MVPA) approaches. Second, we adopted a revised version of inter-temporal choice task and independently manipulated the amount and delay time of future rewards. Using brain-imaging design, reward- and prospection-related brain activations were assessed and their associations with GPT were further examined. The MVPA results showed that GPT was associated with the GMVs in the right lateral frontal pole cortex, left ventromedial prefrontal cortex, right lateral occipital cortex, and right occipital pole. Additionally, we observed that the amount-relevant brain activations (responding to reward circuitry) in the lateral orbitofrontal cortex were negatively associated with individual's variability in GPT scores, whereas the delay time-relevant brain activations (responding to prospection network system) in the dorsolateral prefrontal cortex, dorsomedial prefrontal cortex, superior parietal lobule, and anterior cingulate cortex were positively associated with individual's variability in GPT scores. These findings not only provide novel insights into the neuroanatomical substrates underlying the human dispositional greed, but also suggest the critical roles of reward and prospection processing on the greed.
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http://dx.doi.org/10.1007/s00429-021-02240-9DOI Listing
May 2021

Comparison of mitochondrial genomes from multi-, Bi-, and uninucleate .

Mitochondrial DNA B Resour 2021 Feb 9;6(2):472-474. Epub 2021 Feb 9.

Key Laboratory of Pest Monitoring and Green Management, MOA, Department of Plant Pathology, China Agricultural University, Beijing, China.

Six circular mitochondrial genomes of multi-, bi-, and uninucleate isolates were assembled and found that all the genomes contain 14 conserved protein-coding genes, one ribosomal protein (rps3), and 23 tRNA in the same order. The mitogenome sizes of uninucleate isolates were relatively smaller than binucleate and multinucleate stains. The size variations between uninucleate and multinucleate isolates were from both intergenic and intronic regions, whereas the differences between uninucleate and binucleate isolates were predominantly from intergenic regions. The phylogenetic analysis revealed that strains of the same nucleate types had a closer relationship.
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http://dx.doi.org/10.1080/23802359.2021.1872430DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889275PMC
February 2021

Evolutionary and genomic comparisons of hybrid uninucleate and nonhybrid Rhizoctonia fungi.

Commun Biol 2021 Feb 15;4(1):201. Epub 2021 Feb 15.

Key Laboratory of Pest Monitoring and Green Management, MOA; Joint Laboratory for International Cooperation in Crop Molecular Breeding; Department of Plant Pathology, China Agricultural University, Beijing, China.

The basidiomycetous fungal genus, Rhizoctonia, can cause severe damage to many plants and is composed of multinucleate, binucleate, and uninucleate species differing in pathogenicity. Here we generated chromosome-scale genome assemblies of the three nuclear types of Rhizoctonia isolates. The genomic comparisons revealed that the uninucleate JN strain likely arose by somatic hybridization of two binucleate isolates, and maintained a diploid nucleus. Homeolog gene pairs in the JN genome have experienced both decelerated or accelerated evolution. Homeolog expression dominance occurred between JN subgenomes, in which differentially expressed genes show potentially less evolutionary constraint than the genes without. Analysis of mating-type genes suggested that Rhizoctonia maintains the ancestral tetrapolarity of the Basidiomycota. Long terminal repeat-retrotransposons displayed a reciprocal correlation with the chromosomal GC content in the three chromosome-scale genomes. The more aggressive multinucleate XN strain had more genes encoding enzymes for host cell wall decomposition. These findings demonstrate some evolutionary changes of a recently derived hybrid and in multiple nuclear types of Rhizoctonia.
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http://dx.doi.org/10.1038/s42003-021-01724-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884421PMC
February 2021

The Association Between Antidepressant Effect of SSRIs and Astrocytes: Conceptual Overview and Meta-analysis of the Literature.

Neurochem Res 2021 Feb 1. Epub 2021 Feb 1.

Practical Teaching Centre, School of Forensic Medicine, China Medical University, No. 77, Puhe Street, Shenbei District, Shenyang, 110177, People's Republic of China.

Major depressive disorders (MDD) a worldwide psychiatric disease, is yet to be adequately controlled by therapies; while the mechanisms of action of antidepressants are yet to be fully characterised. In the last two decades, an increasing number of studies have demonstrated the role of astrocytes in the pathophysiology and therapy of MDD. Selective serotonin reuptake inhibitors (SSRIs) are the most widely used antidepressants. It is generally acknowledged that SSRIs increase serotonin levels in the central nervous system by inhibiting serotonin transporters, although the SSRIs action is not ideal. The SSRIs antidepressant effect develops with considerable delay; their efficacy is low and frequent relapses are common. Neither cellular nor molecular pharmacological mechanisms of SSRIs are fully characterised; in particular their action on astrocytes remain underappreciated. In this paper we overview potential therapeutic mechanisms of SSRIs associated with astroglia and report the results of meta-analysis of studies dedicated to MDD, SSRIs and astrocytes. In particular, we argue that fluoxetine, the representative SSRI, improves depressive-like behaviours in animals treated with chronic mild stress and reverses depression-associated decrease in astrocytic glial fibrillary acidic protein (GFAP) expression. In addition, fluoxetine upregulates astrocytic mRNA expression of 5-hydroxytriptamin/serotonin receptors (5-HTR). In summary, we infer that SSRIs exert their anti-depressant effect by regulating several molecular and signalling pathways in astrocytes.
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http://dx.doi.org/10.1007/s11064-020-03225-6DOI Listing
February 2021

A comparative study of immobilizing ammonium molybdophosphate onto cellulose microsphere by radiation post-grafting and hybrid grafting for cesium removal.

Environ Pollut 2021 Jan 5;273:116432. Epub 2021 Jan 5.

State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electric and Electronic Engineering, Huazhong University of Science and Technology, 430074, Wuhan, China. Electronic address:

Ammonium molybdophosphate (AMP) exhibits high selectivity towards Cs but it cannot be directly applied in column packing, so it is necessary to prepare AMP-based adsorbents into an available form to improve its practicality. This work provided two AMP immobilized cellulose microspheres (MCC@AMP and MCC-g-AMP) as adsorbents for Cs removal by radiation grafting technique. MCC-g-AMP was prepared by radiation graft polymerization of GMA on microcrystalline cellulose microspheres (MCC) followed by reaction with AMP suspension, and MCC@AMP was synthesized by radiation hybrid grafting of AMP and GMA onto MCC through one step. The different structures and morphologies of two adsorbents were characterized by FTIR and SEM. The adsorption properties of two adsorbents against Cs were investigated and compared in batch and column experiments under different conditions. Both adsorbents were better obeyed pseudo-second-order kinetic model and Langmuir model. MCC-g-AMP presented faster adsorption kinetic and more stable structure, whereas MCC@AMP presented more facile synthesis and higher adsorption capacity. MCC@AMP was pH independent in the range of pH 1-12 but MCC-g-AMP was sensitive to pH for Cs removal. The saturated column adsorption capacities of MCC@AMP and MCC-g-AMP were 5.4 g-Cs/L-ad and 0.75 g-Cs/L-ad in column adsorption experiments at SV 10 h. Both adsorbents exhibited very high radiation stability and can maintain an adsorption capacity of >85% even after 1000 kGy γ-irradiation. On the basis, two AMP-loaded adsorbents possessed promising application in removal of Cs from actual contaminated groundwater. These findings provided two efficient adsorbents for treatment of Cs in radioactive waste disposal.
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http://dx.doi.org/10.1016/j.envpol.2021.116432DOI Listing
January 2021

Comparing the prognostic significance of nutritional screening tools and ESPEN-DCM on 3-month and 12-month outcomes in stroke patients.

Clin Nutr 2020 Nov 7. Epub 2020 Nov 7.

Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address:

Background And Aims: Malnutrition is prevalent among individuals with acute ischaemic stroke (AIS) and may worsen clinical outcomes. There is no consensus on the best tool for nutritional screening in this population. The present study compared four screening tools and one diagnostic tool in terms of their prognostic significance in predicting short-term and long-term outcomes in AIS patients.

Methods: We included patients admitted to five major hospitals in Wenzhou and diagnosed with a primary diagnosis of AIS from October 1 to December 31, 2018. The Controlling Nutritional Status (CONUT) score, the Geriatric Nutritional Risk Index (GNRI), the Malnutrition Universal Screening Tool (MUST), the Nutritional Risk Screening Tool 2002 (NRS-2002) and the European Society for Clinical Nutrition and Metabolism diagnostic criteria for malnutrition (ESPEN-DCM) were assessed at admission. The clinical outcomes were evaluated by the modified Rankin Scale (mRS) and mortality at 3 months and 12 months after discharge.

Results: Five hundred and ninety-three patients were included in our prospective study. The mean age was 67.3 ± 12.0 years. Based on the mRS score, 125 patients exhibited poor functional recovery (an mRS ≥3) at 3 months after discharge. Seventeen patients died during the 3-month follow-up period, and the other 25 did not survive 12 months. Multivariate binary logistic regression revealed that inadequate nutritional status at admission, as determined by the CONUT, GNRI, MUST, NRS-2002 and ESPEN-DCM, were independently associated with poor outcomes in AIS patients 3 months after discharge. Both MUST ≥2 and NRS-2002 ≥ 3 showed significant associations with poor outcomes at 12-month post-discharge. Further analysis with the receiver operator characteristic (ROC) curve showed similar results, where all the tools predicted the poor outcomes at 3 months while only the NRS-2002 and MUST scores were significantly associated with the mRS at 12 months post-discharge. Moreover, the area under the curve (AUC) of MUST and NRS-2002 were significantly larger than those for the other tools. The optimal cut-off values of the MUST and NRS-2002 to predict poor outcomes were scores of ≥2 and ≥ 3 points, respectively.

Conclusions: Our data supported a deleterious effect of inadequate nutrition, as evidenced by the nutrition screening tools or ESPEN-DCM, on clinical outcomes during and beyond the acute phase of AIS. We recommended the use of the MUST and NRS-2002 in guiding nutritional support in AIS patients, as they have higher predictive power and can predict both short-term and long-term outcomes.
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http://dx.doi.org/10.1016/j.clnu.2020.11.001DOI Listing
November 2020

Identification and Bioinformatic Assessment of circRNA Expression After Knockdown and Ionizing Radiation Exposure.

DNA Cell Biol 2021 Jan 17;40(1):80-92. Epub 2020 Nov 17.

Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.

RecQ-mediated genome instability protein 1 (RMI1) is an important component of the BLM-Topo IIIα-RMI1-RMI2 complex and plays a critical role in maintaining genome stability. However, the cellular functions of RMI1 in response to ionizing radiation (IR) are poorly understood. In this study, we found that knockdown led to enhanced radiosensitivity and apoptosis after irradiation. To analyze the effect of knockdown on the expression of circular RNAs (circRNAs), we performed high-throughput RNA sequencing on four groups of human embryonic kidney (HEK) 293T cells: control cells and knockdown cells with or without IR exposure. A total of 179 and 160 differentially expressed circRNAs (DE-circRNAs) were identified under knockdown without and with exposure to IR, respectively. The Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed that these DE-circRNAs were involved in a variety of functions and signal pathways, including histone H3-K36 methylation, nuclear pore organization, mRNA destabilization, the mismatch repair pathway, and the apoptotic signaling pathway. Overall, our results indicate that RMI1 plays a crucial role in the response to IR and, more generally, that circRNAs are important in the regulatory mechanism of the radiation response.
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http://dx.doi.org/10.1089/dna.2020.5976DOI Listing
January 2021

Desuccinylation-Triggered Peptide Self-Assembly: Live Cell Imaging of SIRT5 Activity and Mitochondrial Activity Modulation.

J Am Chem Soc 2020 10 11;142(42):18150-18159. Epub 2020 Oct 11.

Department of Chemistry and COSDAF (Centre of Super-Diamond and Advanced Films), City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.

Mimicking nature's ability to orchestrate molecular self-assembly in living cells is important yet challenging. Molecular self-assembly has found wide applications in cellular activity control, drug delivery, biomarker imaging, etc. Nonetheless, examples of suborganelle-confined supramolecular self-assembly are quite rare and research in this area remains challenging. Herein, we have presented a new strategy to program supramolecular self-assembly specifically in mitochondria by leveraging on a unique enzyme SIRT5. SIRT5 is a mitochondria-localized enzyme belonging to a family of NAD-dependent histone deacetylases. Accumulating studies suggest that SIRT5 is involved in regulating diverse biological processes, such as reactive oxygen defense, fatty acid metabolism, and apoptosis. In this study, we designed a novel class of succinylated peptide precursors that can be transformed into self-assembling building blocks through SIRT5 catalysis, leading to the formation of supramolecular nanofibers in vitro and in living cells. The increased hydrophobicity arising from self-assembly remarkably enhanced the fluorescence of nitrobenzoxadiazole (NBD) in the nanofibers. With this approach, we have enabled activity-based imaging of SIRT5 in living cells for the first time. Moreover, SIRT5-mediated peptide self-assembly was found to depolarize mitochondria membrane potential and promote ROS formation. Coincubation of the peptide with three different chemotherapeutic agents significantly boosted the anticancer activities of these drugs. Our work has thus illustrated a new way of mitochondria-confined peptide self-assembly for SIRT5 imaging and potential anticancer treatment.
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http://dx.doi.org/10.1021/jacs.0c08463DOI Listing
October 2020

Profiling of rice Cd-tolerant genes through yeast-based cDNA library survival screening.

Plant Physiol Biochem 2020 Oct 9;155:429-436. Epub 2020 Aug 9.

National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing, China. Electronic address:

The bioaccumulation of cadmium (Cd) in crop and the subsequent food chain has aroused extensive concerns. However, the underlying molecular mechanisms of plant Cd tolerance remain to be clarified from the viewpoint of novel candidate genes. Here we described a highly efficient approach for preliminary identifying rice Cd-tolerant genes through the yeast-based cDNA library survival screening combined with high-throughput sequencing strategy. About 690 gene isoforms were identified as being Cd-tolerant candidates using this shotgun approach. Among the Cd-tolerant genes identified, several categories of genes such as BAX inhibitor (BI), NAC transcription factors and Rapid ALkalinization Factors (RALFs) were of particular interest, and their function of Cd tolerance was further validated via heterologous expression, which suggested that SNAC1, RALF12, OsBI-1 can confer Cd tolerance in yeast and tobacco leaves. Regarding the genes involved in ion transport, the validated Cd-tolerant heavy metal-associated domain (HMAD) isoprenylated protein HIPP42 was particularly noteworthy. Further elucidation of these genes associated with Cd tolerance in rice will benefit agricultural activities.
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http://dx.doi.org/10.1016/j.plaphy.2020.07.046DOI Listing
October 2020

Morphology and transport characterization of solution-processed rubrene thin films on polymer-modified substrates.

Sci Rep 2020 Jul 22;10(1):12183. Epub 2020 Jul 22.

School of Material Science and Engineering, Zhengzhou University, Zhengzhou, 450001, China.

In this report, the morpho-structural peculiarities and the crystallization mechanisms in solution-processed, solvent vapor annealed (SVA) thin films of rubrene (5,6,11,12-tetraphenylnaphthacene) on different substrates were investigated. The high-quality rubrene crystal films with a triclinic crystal structure were successfully prepared on the FTO substrates (glass slide coated with fluorine-tin-oxide) modified by PLA (polylactic acid) for the first time. The area coverage of rubrene crystal and the sizes of rubrene dendritic crystals increased with increasing thickness of PLA film and concentration of rubrene solution. For rubrene molecules, FTO wafers with rough surface provided the possibility of heterogeneous nucleation. During the SVA process, there were two kinds of forces acting on the diffusion of rubrene molecules: one force was provided by the residual chloroform solvent, which was perpendicular to the substrate, and the other force was provided by gaseous dichloromethane, which was parallel to the substrate. The synergy of these two forces was proposed to explain the nucleation and the crystallization processes of rubrene films. The higher nucleus of PLA/rubrene dendrites and the layer-by-layer stacking of needle-shaped nanocrystalline PLA/rubrene were important for exploring their kinetic formation process.
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http://dx.doi.org/10.1038/s41598-020-68293-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376014PMC
July 2020

The Protection Effect of Resveratrol Against Radiation-Induced Inflammatory Bowel Disease via NLRP-3 Inflammasome Repression in Mice.

Dose Response 2020 Apr-Jun;18(2):1559325820931292. Epub 2020 Jun 26.

Laboratory of Radiation Medicine and radiation injury effects, Tianjin Institute of Radiology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin, China.

With the extensive application of radiotherapy in various cancers, its side effects in tissues adjacent to cancers are garnering much attention. Intestines are sensitive to irradiation due to its rapid proliferation, and irradiation-induced enteric inflammation is common in patients with pelvic peritoneal tumors. Sirt1, class III protein deacetylase, could lead to transcriptional repression of various inflammation-associated genes, and our previous study has proved its relationship with interleukin (IL)-1β. Here we show that resveratrol, the activator of Sirt1, could alleviate the bowel inflammation induced by irradiation and the expression of Sirt1 is consistent with the inflammation level. We further identified in vivo that Sirt1 repress the expression of IL-1β by the repression of NLR Family, Pyrin Domain Containing protein 3 (NLRP3) expression. In conclusion, this study confirms resveratrol acts against radiation-induced inflammatory bowel disease via NLRP-3 inflammasome repression in mice and supports Sirt1 as a potential biomarker and therapy target in intestinal radiation protection.
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http://dx.doi.org/10.1177/1559325820931292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7323307PMC
June 2020

Exome sequencing identifies somatic mutations in novel driver genes in non-small cell lung cancer.

Aging (Albany NY) 2020 07 6;12(13):13701-13715. Epub 2020 Jul 6.

Department of Respiration, Yangpu Hospital, Tongji University School of Medicine, Shanghai, China.

Lung cancer is the leading cause of cancer death worldwide and accounts for more than one-third of all newly diagnosed cancer cases in China. Therefore, it is of great clinical significance to explore new driver gene mutations in non-small-cell lung cancer (NSCLC). Using an initial bioinformatic analysis, we identified somatic gene mutations in 13 patients with NSCLC and confirmed these mutations by targeted sequencing in an extended validation group of 88 patients. Recurrent mutations were detected in (7.9%), (5.0%), (4.0%), (2.0%), and (2.0%). A functional study was also performed in UNC5D mutants. Mutations in promoted tumorigenesis by abolishing the tumor suppressor function of the encoded protein. Additionally, in ten patients with lung squamous cell carcinoma, we identified mutations in that influenced the expression of target genes and . Overall, the results of our study expanded the known spectrum of driver mutations involved in the pathogenesis of NSCLC.
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http://dx.doi.org/10.18632/aging.103500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377869PMC
July 2020

Iron Aggravates the Depressive Phenotype of Stressed Mice by Compromising the Glymphatic System.

Neurosci Bull 2020 12 24;36(12):1542-1546. Epub 2020 Jun 24.

Practical Teaching Center, School of Forensic Medicine, China Medical University, Shenyang, 110122, China.

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http://dx.doi.org/10.1007/s12264-020-00539-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719143PMC
December 2020

Sleep Deprivation Selectively Down-Regulates Astrocytic 5-HT Receptors and Triggers Depressive-Like Behaviors via Stimulating P2X Receptors in Mice.

Neurosci Bull 2020 Nov 6;36(11):1259-1270. Epub 2020 Jun 6.

Practical Teaching Centre, School of Forensic Medicine, China Medical University, Shenyang, 110122, China.

Chronic loss of sleep damages health and disturbs the quality of life. Long-lasting sleep deprivation (SD) as well as sleep abnormalities are substantial risk factors for major depressive disorder, although the underlying mechanisms are not clear. Here, we showed that chronic SD in mice promotes a gradual elevation of extracellular ATP, which activates astroglial P2X receptors (P2XRs). Activated P2XRs, in turn, selectively down-regulated the expression of 5-HT receptors (5-HTRs) in astrocytes. Stimulation of P2XRs induced by SD selectively suppressed the phosphorylation of AKT and FoxO3a in astrocytes, but not in neurons. The over-expression of FoxO3a in astrocytes inhibited the expression of 5-HTRs. Down-regulation of 5-HTRs instigated by SD suppressed the activation of STAT3 and relieved the inhibition of Ca-dependent phospholipase A2. This latter cascade promoted the release of arachidonic acid and prostaglandin E2. The depression-like behaviors induced by SD were alleviated in P2XR-KO mice. Our study reveals the mechanism underlying chronic SD-induced depression-like behaviors and suggests 5-HTRs as a key target for exploring therapeutic strategies aimed at the depression evoked by sleep disorders.
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http://dx.doi.org/10.1007/s12264-020-00524-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674526PMC
November 2020

Atractylenolide II prevents radiation damage via MAPKp38/Nrf2 signaling pathway.

Biochem Pharmacol 2020 07 30;177:114007. Epub 2020 Apr 30.

Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China. Electronic address:

Ionizing radiation (IR) can act as a negative factor for human homeostasis, by causing and even aggravating a series of pathological conditions. To protect the intactness of normal tissues, effective anti-radiation drugs are urgently needed for alleviating the outcomes of radioactive damage. In this study, we demonstrate that atractylenolide II (ATR II), a sesquiterpenoid monomer extracted from traditional Chinese medicine atractylodes macrocephala, can markedly suppress IR damage by promoting the expression of antioxidant factors heme oxygenase-1 (HO-1) and NAD(P)H dehydrogenase quinone oxido-reductase 1 (NQO-1), which are mediated by nuclear factor-erythroid 2-like 2 (Nrf2) signaling pathway. Furthermore, here we reveal that ATR II effectively upregulates the expression of mitogen-activated protein kinase p38 (MAPKp38), which also acts as a regulator of Nrf2 signaling cascade. Indeed, treatment with a MAPKp38 inhibitor can significantly downregulate the expression of Nrf2 and its downstream target genes HO-1 and NQO-1 and, consequently, abolish the protective effect of ATR II against IR. Consistently, ATR II also has a protective function against IR-induced damage in animal models. In conclusion, our study provides an unexpected function of ATR II in preventing IR-induced damage by modulating MAPKp38/Nrf2 signaling pathway.
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http://dx.doi.org/10.1016/j.bcp.2020.114007DOI Listing
July 2020

A DFT study on the photoelectric properties of rubrene and its derivatives.

J Mol Model 2020 Jan 22;26(2):32. Epub 2020 Jan 22.

School of Chemistry and Molecular Engineering, Zhengzhou University, No. 100 Science Avenue, Zhengzhou 450001, Henan, People's Republic of China.

The way to obtain new materials was usually to introduce some groups to molecules. Correlations among the molecular structure and photoelectric properties of rubrene and its eight derivatives have been studied in this paper, and the influences of the introduction of different electron-donating and withdrawing substituents on the molecular orbital, reorganization energies, absorption spectra, and fluorescence spectra of rubrene and its derivatives were discussed. In the present work, density functional theory calculations were performed at the B3LYP/6-311G(d,p) level to optimize the structure, and TD-DFT was used to calculate the absorption and emission spectra. Quantum chemistry calculation results indicated that the maximum absorption wavelengths are redshifted due to the introduction of cyclopentadienyl or furan groups, the maximum absorption wavelengths are blueshifted while methoxy groups substituted on tetracene backbone. We also discussed the influence of substituents on the molecule structure, which suggests that introducing furan and cyclopentadienyl substituents on the tetracene backbone can increase the rigidity of rubrene and improve the fluorescence intensity. The results of reorganization show that the introduction of cyclopentadienyl or furan groups into rubrene is advantageous to the holes transportation, and the introduction of F groups contributes to the electrons transportation. This study provides an insight into the properties of rubrene and its derivatives and supplies an effective method to design new organic semiconductor materials.
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http://dx.doi.org/10.1007/s00894-020-4295-xDOI Listing
January 2020

Fluoxetine improves behavioural deficits induced by chronic alcohol treatment by alleviating RNA editing of 5-HT receptors.

Neurochem Int 2020 03 20;134:104689. Epub 2020 Jan 20.

Practical Teaching Centre, School of Forensic Medicine, China Medical University, Shenyang, PR China. Electronic address:

The alcoholism and major depressive disorder are common comorbidity, with alcohol-induced depressive symptoms being eased by selective serotonin re-uptake inhibitors (SSRIs), although the mechanisms underlying pathology and therapy are poorly understood. Chronic alcohol consumption affects the activity of serotonin 2C receptors (5-HTR) by regulating adenosine deaminases acting on RNA (ADARs) in neurons. Astrogliopathic changes contribute to alcohol addiction, while decreased release of ATP from astrocytes can trigger depressive-like behaviours in mice. In this study, we discovered that chronic alcohol treatment increased editing of RNA of 5-HTR via up-regulating the expression of ADAR2, consequently reducing the release of ATP from astrocytes induced by 5-HTR agonist, MK212. Moreover, SSRI antidepressant fluoxetine decreased the expression of ADAR2 through the transactivation of EGFR/PI3K/AKT/cFos signalling pathway. The increased release of astroglial ATP by MK212 which was suppressed by chronic alcohol consumption, and reduction in ADAR2 activity eliminated the RNA editing of 5-HTR increased by alcohol in vitro and recovered the release of ATP from astrocytes induced by MK212. Meanwhile, fluoxetine improved the behavioural and motor symptoms induced by alcohol addiction and decreased the alcohol intake. Our study suggests that the astrocytic 5-HTR contribute to alcohol addiction; fluoxetine thus can be used to alleviate depression, treat alcohol addiction and improve motor coordination.
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http://dx.doi.org/10.1016/j.neuint.2020.104689DOI Listing
March 2020

A co-registration investigation of inter-word spacing and parafoveal preview: Eye movements and fixation-related potentials.

PLoS One 2019 18;14(12):e0225819. Epub 2019 Dec 18.

School of Psychology, University of Central Lancashire, Preston, United Kingdom.

Participants' eye movements (EMs) and EEG signal were simultaneously recorded to examine foveal and parafoveal processing during sentence reading. All the words in the sentence were manipulated for inter-word spacing (intact spaces vs. spaces replaced by a random letter) and parafoveal preview (identical preview vs. random letter string preview). We observed disruption for unspaced text and invalid preview conditions in both EMs and fixation-related potentials (FRPs). Unspaced and invalid preview conditions received longer reading times than spaced and valid preview conditions. In addition, the FRP data showed that unspaced previews disrupted reading in earlier time windows of analysis, compared to string preview conditions. Moreover, the effect of parafoveal preview was greater for spaced relative to unspaced conditions, in both EMs and FRPs. These findings replicate well-established preview effects, provide novel insight into the neural correlates of reading with and without inter-word spacing and suggest that spatial selection precedes lexical processing.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0225819PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6919607PMC
April 2020

Radiation Responses of Human Mesenchymal Stem Cells Derived From Different Sources.

Dose Response 2019 Oct-Dec;17(4):1559325819893210. Epub 2019 Dec 9.

Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Department of Radiobiology, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin, China.

Mesenchymal stem cells (MSCs) derived from different tissues may aid in the regeneration of radiation-induced organ lesions; however, the radiation responses of human MSCs from different sources are unknown. In our study, a comparison of the results from cell proliferation, apoptosis, cell cycle, DNA damage, and DNA repair assays consistently showed that MSCs derived from adipose tissue possess a significantly stronger radiation resistance capacity than MSCs derived from umbilical cord and gingival, which is accompanied by a higher level of phosphorylated signal transducer and activator of transcription 3 (Stat3) expression. This reminds us Stat3 could be a potential biomarker of radiation resistance. These findings provide a better understanding of radiation-induced biologic responses in MSCs and may lead to the development of better strategies for stem cell treatment and cancer therapy.
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http://dx.doi.org/10.1177/1559325819893210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902398PMC
December 2019

Eye Movements Reveal Delayed Use of Construction-Based Pragmatic Information During Online Sentence Reading: A Case of Chinese … Construction.

Front Psychol 2019 30;10:2211. Epub 2019 Oct 30.

School of Psychological and Cognitive Sciences, Peking University, Beijing, China.

An event-related potential (ERP) study demonstrated that construction-based pragmatic constraints in Chinese (e.g., … that constrains a low-likelihood event and is similar to in English) can rapidly influence sentence comprehension and the mismatch of such constraints would lead to increased neural activity on the mismatching word. Here we examine to what extent readers' eye movements can instantly reveal the difficulties of mismatching constraints when participants read sentences with the structure + + + + + + . By embedding high-likelihood or neutral events in the construction, we created incongruent and underspecified sentences and compared such sentences with congruent ones describing events of low expectedness. Relative to congruent sentences, the VP region of incongruent sentences showed no significant differences on first-pass reading time measures, but the total fixation duration was reliably longer. Moreover, readers made more regressions from the VP and the sentence-final region to previous regions in the incongruent than the congruent condition. These findings suggest that the effect of pragmatic constraints is observable during naturalistic sentence reading, reflecting the activation of the construction-based pragmatic information for the late integration of linguistic and extra-linguistic information at sentential level.
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http://dx.doi.org/10.3389/fpsyg.2019.02211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6842951PMC
October 2019

Maize NAC-domain retained splice variants act as dominant negatives to interfere with the full-length NAC counterparts.

Plant Sci 2019 Dec 7;289:110256. Epub 2019 Sep 7.

National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

The plant-specific NAC transcription factors play diverse roles in various stress signaling. Alternative splicing is particularly prevalent in plants under stress. However, the investigation of cadmium (Cd) on the differential expression of the splice variants of NACs is in its infancy. Here, we identified three Cd-induced intron retention splice NAC variants which only contained the canonical NAC domain, designated as nacDomains, derived from three Cd-upregulated maize NACs. Subcellular localization analysis indicated that both nacDomain and its full-length NAC counterpart co-localized in the nucleus as manifested in the BiFC assay, thus implied that nacDomains and their corresponding NACs form heterodimers through the identical NAC domain. Further chimeric reporter/effector transient expression assay and Cd-tolerance assay in tobacco leaves collectively indicated that nacDomain-NAC heterodimers were involved in the regulation of NAC function. The results obtained here were in accordance with the model of dominant negative, which suggested that nacDomain act as the dominant negative to antagonize the regulation of NAC on its target gene expression and the Cd-tolerance function performance of NAC transcription factor. These findings proposed a novel insight into understanding the molecular mechanisms of Cd response in plants.
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http://dx.doi.org/10.1016/j.plantsci.2019.110256DOI Listing
December 2019

ZBTB20 promotes cell migration and invasion of gastric cancer by inhibiting IκBα to induce NF-κB activation.

Artif Cells Nanomed Biotechnol 2019 Dec;47(1):3862-3872

Department of Oncology, Xuzhou Central Hospital, Xuzhou Medical University , Xuzhou , China.

Zinc finger and BTB domain containing 20 (ZBTB20), a sequence-specific transcriptional repressor, has been found to be involved in tumorigenesis. However, its role(s) in gastric cancer and the molecular mechanisms involved are poorly investigated. Here, our data demonstrated that ZBTB20 expression was markedly upregulated in gastric cancer cell lines infected with () and in gastric cancer tumor samples. Loss- and gain-of-function studies showed that ZBTB20 promoted cell proliferation, invasion and migration of gastric cancer cell lines. Mechanistically, the phosphorylation of NF-κBp65 and expression and activity of MMP-2 and MMP-9 were increased, while IκBα expression was decreased by ZBTB20 in gastric cancer cells. We further revealed that IκBα overexpression significantly inhibited NF-κB signaling as well as cell migration, invasion and proliferation in gastric cancer cell lines induced by ZBTB20 overexpression. Therefore, our findings emphasize an important role for ZBTB20 in controlling gastric cancer development, which is helpful to identify potential therapeutic targets for its treatment.
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http://dx.doi.org/10.1080/21691401.2019.1670188DOI Listing
December 2019

Methylene Blue-Mediated Photodynamic Therapy Induces Macrophage Apoptosis via ROS and Reduces Bone Resorption in Periodontitis.

Oxid Med Cell Longev 2019 14;2019:1529520. Epub 2019 Aug 14.

Department of Prosthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Aim: To investigate whether methylene blue-mediated photodynamic therapy (MB-PDT) can affect the "fate" of macrophages or in periodontitis tissues and to explore the potential mechanism.

Methods: For treatments, THP-1 macrophages were divided into three experimental groups: C/control, no treatment; MB, methylene blue treatment; and MB-PDT, MB and laser irradiation treatment. Then, apoptosis and apoptosis-related proteins were detected in each group. For treatments, periodontitis was ligature-induced in the first molars of the bilateral maxilla in 12 Sprague Dawley (SD) rats. After six weeks, the ligatures were removed and all the induced molars underwent scaling and root planning (SRP). Then, the rats were divided into three groups according to the following treatments: SRP, saline solution; MB, phenothiazinium dye; and MB-PDT, MB and laser irradiation. Apoptotic macrophages, inflammation levels, and alveolar bone resorption in the periodontal tissues of rats were analyzed in each group.

Results: , flow cytometry analysis demonstrated that 10 M MB and 40 J/cm laser irradiation maximized the apoptosis rate (34.74%) in macrophages. Fluorescence probe and Western blot analyses showed that MB-PDT induced macrophage apoptosis via reactive oxygen species (ROS) and the mitochondrial-dependent apoptotic pathway. Conversely, the addition of exogenous antioxidant glutathione (GSH) and the pan-caspase inhibitor Z-VAD-FMK markedly reduced the apoptotic response in macrophages. , immunohistochemistry, histology, radiographic, and molecular biology experiments revealed fewer infiltrated macrophages, less bone loss, and lower IL-1 and TNF- levels in the MB-PDT group than in the SRP and MB groups ( < 0.05). Immunohistochemistry analysis also detected apoptotic macrophages in the MB-PDT group.

Conclusion: MB-PDT could induce macrophage apoptosis and in rats with periodontitis. This may be another way for MB-PDT to relieve periodontitis in addition to its antimicrobial effect. Meanwhile, MB-PDT induced apoptosis in THP-1 macrophages via the mitochondrial caspase pathway.
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http://dx.doi.org/10.1155/2019/1529520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710739PMC
January 2020

Simultaneous detection of five flavoring agents in chewing gum by ultrasound-microwave synergistic extraction coupled with gas chromatography.

Sci Rep 2019 08 19;9(1):12085. Epub 2019 Aug 19.

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China.

So far, the identification and determination of flavor additives in food has gained extensive attention in the area of food safety. However, it remains a big challenge for simultaneous detection of diverse flavor agents. In this work, a novel gas chromatography method coupled with ultrasound-microwave synergistic extraction was developed for simultaneous detection of five flavor compounds, including butyl butyrate, menthol, methyl salicylate, eugenol and vanilline. In this strategy, ultrasound-microwave synergistic extraction was used to extract the five flavoring agents from chewing gum. The effects of extractants, solid-liquid ratio, extraction time and microwave power on extraction yield were researched by using orthogonal test. After the optimization of programme temperature and splitless injection, the five flavoring agents were well separated and simultaneously detected with wide linear ranges, low limits of detection, high accuracy and good repeatability. Therefore, this proposed method would hold great promise for assay application on the food safety.
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http://dx.doi.org/10.1038/s41598-019-48522-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700314PMC
August 2019

Regulation of Glutarate Catabolism by GntR Family Regulator CsiR and LysR Family Regulator GcdR in Pseudomonas putida KT2440.

mBio 2019 07 30;10(4). Epub 2019 Jul 30.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of China

Glutarate, a metabolic intermediate in the catabolism of several amino acids and aromatic compounds, can be catabolized through both the glutarate hydroxylation pathway and the glutaryl-coenzyme A (glutaryl-CoA) dehydrogenation pathway in KT2440. The elucidation of the regulatory mechanism could greatly aid in the design of biotechnological alternatives for glutarate production. In this study, it was found that a GntR family protein, CsiR, and a LysR family protein, GcdR, regulate the catabolism of glutarate by repressing the transcription of and , two key genes in the glutarate hydroxylation pathway, and by activating the transcription of and , two key genes in the glutaryl-CoA dehydrogenation pathway, respectively. Our data suggest that CsiR and GcdR are independent and that there is no cross-regulation between the two pathways. l-2-Hydroxyglutarate (l-2-HG), a metabolic intermediate in the glutarate catabolism with various physiological functions, has never been elucidated in terms of its metabolic regulation. Here, we reveal that two molecules, glutarate and l-2-HG, act as effectors of CsiR and that KT2440 uses CsiR to sense glutarate and l-2-HG and to utilize them effectively. This report broadens our understanding of the bacterial regulatory mechanisms of glutarate and l-2-HG catabolism and may help to identify regulators of l-2-HG catabolism in other species. Glutarate is an attractive dicarboxylate with various applications. Clarification of the regulatory mechanism of glutarate catabolism could help to block the glutarate catabolic pathways, thereby improving glutarate production through biotechnological routes. Glutarate is a toxic metabolite in humans, and its accumulation leads to a hereditary metabolic disorder, glutaric aciduria type I. The elucidation of the functions of CsiR and GcdR as regulators that respond to glutarate could help in the design of glutarate biosensors for the rapid detection of glutarate in patients with glutaric aciduria type I. In addition, CsiR was identified as a regulator that also regulates l-2-HG metabolism. The identification of CsiR as a regulator that responds to l-2-HG could help in the discovery and investigation of other regulatory proteins involved in l-2-HG catabolism.
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http://dx.doi.org/10.1128/mBio.01570-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6667623PMC
July 2019

Distinct profiles of cognitive impairment associated with different silent cerebrovascular lesions in hypertensive elderly Chinese.

J Neurol Sci 2019 Aug 28;403:139-145. Epub 2019 Jun 28.

Department of Medicine, The University of Hong Kong, Hong Kong; Research Centre of Heart, Brain, Hormone & Healthy Aging, The University of Hong Kong, Hong Kong. Electronic address:

Background/objectives: Silent cerebrovascular lesions (SCLs) and their underlying pathology are now recognized as important causes of cognitive impairment in the elderly. However, the distinct profile of cognitive deficits associated with each type of SCLs remains unclear.

Methods: Of 497 otherwise healthy hypertensive elderly Chinese, 398 participants (mean age 72.0, ranging from 65 to 99, SD = 5.1) successfully completed a battery of structured neuropsychological tests and a multi-sequence 3 T MRI scanning. SCLs were rated independently. Correlations between each MRI marker and cognitive function were assessed using a series of linear regression models.

Results: Strictly lobar cerebral microbleeds were linked to impaired language function (B = -0.231, p < 0.05). Silent lacunes were associated with poor executive function, but the association disappeared after additional adjustment for white matter hyperintensities. White matter hyperintensities (especially periventricular hyperintensities) were associated with poor executive function (B = -0.126, p < 0.05) and slower information processing speed (B = -0.149, p < 0.05).

Conclusion: Different SCLs were associated with different patterns of cognitive deficits, indicating that different SCLs may have distinct impacts on cognitive performance.
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http://dx.doi.org/10.1016/j.jns.2019.06.028DOI Listing
August 2019

Integrated Analysis of the Altered lncRNAs and mRNAs Expression in 293T Cells after Ionizing Radiation Exposure.

Int J Mol Sci 2019 Jun 18;20(12). Epub 2019 Jun 18.

Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China.

Tissue and cell damage caused by ionizing radiation is often highly genotoxic. The swift repair of DNA damage is crucial for the maintenance of genomic stability and normal cell fitness. Long noncoding RNAs (lncRNAs) have been reported to play an important role in many physiological and pathological processes in cells. However, the exact function of lncRNAs in radiation-induced DNA damage has yet to be elucidated. Therefore, this study aimed to analyze the potential role of lncRNAs in radiation-induced DNA damage. We examined the expression profiles of lncRNAs and mRNAs in 293T cells with or without 8 Gy irradiation using high-throughput RNA sequencing. We then performed comprehensive transcriptomic and bioinformatic analyses of these sequencing results. A total of 18,990 lncRNAs and 16,080 mRNAs were detected in all samples. At 24 h post irradiation, 49 lncRNAs and 323 mRNAs were differentially expressed between the irradiation group and the control group. qRT-PCR was used to verify the altered expression of six lncRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that the predicted genes were mainly involved in the histone mRNA metabolic process and Wnt signaling pathways. This study may provide novel insights for the study of lncRNAs in radiation-induced DNA damage.
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http://dx.doi.org/10.3390/ijms20122968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627384PMC
June 2019

Transcriptional regulation of MdPIN3 and MdPIN10 by MdFLP during apple self-rooted stock adventitious root gravitropism.

BMC Plant Biol 2019 May 30;19(1):229. Epub 2019 May 30.

State Key Laboratory of Crop Biology; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), Ministry of Agriculture; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, Shandong, China.

Background: The close planting of dwarfing self-rooted rootstocks is currently a widely used method for apple production; however, self-rooted rootstocks are weak with shallow roots and poor grounding. Therefore, understanding the molecular mechanisms that establish the gravitropic set-point angles (GSAs) of the adventitious roots of self-rooted apple stocks is important for developing self-rooted apple rootstock cultivars with deep roots.

Results: We report that the apple FOUR LIPS (MdFLP), an R2R3-MYB transcription factor (TF), functions in establishing the GSA of the adventitious roots of self-rooted apple stocks in response to gravity. Biochemical analyses demonstrate that MdFLP directly binds to the promoters of two auxin efflux carriers, MdPIN3 and MdPIN10, that are involved in auxin transport, activates their transcriptional expression, and thereby promotes the development of adventitious roots in self-rooted apple stocks. Additionally, the apple auxin response factor MdARF19 influences the expression of those auxin efflux carriers and the establishment of the GSA of adventitious roots of apple in response to gravity by directly activating the expression of MdFLP.

Conclusion: Our findings provide new insights into the transcriptional regulation of MdFLP by the auxin response factor MdARF19 in the regulation of the GSA of adventitious roots of self-rooted apple stocks in response to gravity.
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http://dx.doi.org/10.1186/s12870-019-1847-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6543673PMC
May 2019

The influence of foveal lexical processing load on parafoveal preview and saccadic targeting during Chinese reading.

J Exp Psychol Hum Percept Perform 2019 Jun;45(6):812-825

School of Psychology.

Whether increased foveal load causes a reduction of parafoveal processing remains equivocal. The present study examined foveal load effects on parafoveal processing in natural Chinese reading. Parafoveal preview of a single-character parafoveal target word was manipulated by using the boundary paradigm (Rayner, 1975; pseudocharacter or identity previews) under high foveal load (low-frequency pretarget word) compared with low foveal load (high-frequency pretarget word) conditions. Despite an effective manipulation of foveal processing load, we obtained no evidence of any modulatory influence on parafoveal processing in first-pass reading times. However, our results clearly showed that saccadic targeting, in relation to forward saccade length from the pretarget word and in relation to target word skipping, was influenced by foveal load and this influence occurred independent of parafoveal preview. Given the optimal experimental conditions, these results provide very strong evidence that preview benefit is not modulated by foveal lexical load during Chinese reading. (PsycINFO Database Record (c) 2019 APA, all rights reserved).
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http://dx.doi.org/10.1037/xhp0000644DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532562PMC
June 2019

Pharmacokinetic study of rosavin in rat plasma with ultra performance LC-MS/MS after intravenous and gavage administration.

Bioanalysis 2019 May 16;11(9):837-845. Epub 2019 May 16.

Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, PR China.

A sensitive and selective ultra performance LC-MS/MS (UPLC-MS/MS) method was developed to investigate the pharmacokinetics of rosavin as a potential adaptogenic drug isolated from in rat plasma with salidroside as an internal standard. Chromatographic separation was performed on a UPLC HSS T3 column (1.8 μm, 100 mm × 2.1 mm) with gradient elution. Multiple reaction monitoring was employed for MS analysis. Rosavin and salidroside were determined with multiple reaction monitoring-ion transitions 427.2 → 293.1 and 299.1 → 119.1, respectively. The validated UPLC-MS/MS method showed a satisfied linear range in 5-5000 ng/ml and was successfully applied for the pharmacokinetic study of rosavin in the rat after intravenous and gavage administration.
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http://dx.doi.org/10.4155/bio-2019-0022DOI Listing
May 2019