Dr Ravishankar Ram Mani, PhD - University of Malaya - Senior Research Fellow

Dr Ravishankar Ram Mani

PhD

University of Malaya

Senior Research Fellow

Kuala Lumpur, Kuala Lumpur | Malaysia

Main Specialties: Biochemical Genetics, Clinical & Laboratory Immunology, Gastroenterology, Hepatology, Infectious Disease, Medical Genetics, Medical Microbiology, Molecular Genetic Pathology, Pharmacology, Statistics

Additional Specialties: Molecular Cell Biology

ORCID logohttps://orcid.org/0000-0003-4229-5778


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Dr Ravishankar Ram Mani, PhD - University of Malaya - Senior Research Fellow

Dr Ravishankar Ram Mani

PhD

Introduction

Currently, I am associated with the Faculty of Science, University of Malaya, under Prof. Subha Bhassu & Faculty of Medicine, under Prof. Suresh, involving Multicentric Projects. We are collaborating with the University of Charles Darwin, NYU School of Medicine, New York State University, Pasteur Institute, France and Indian Council of Medical Research (ICMR), India. In my Ph.D. program, I have contributed towards the study of Molecular cell biology in which explains on expression of leptin (ob gene product) and the cytokines tumor necrosis factor-alpha, interleukins 6 and 8 in a reproductive system with special reference to the polycystic ovarian syndrome in Indian population. I have 18 years of experience at the bench as molecular biology and I am an expert in the new CRISPR/Cas9 technology for genome editing. Specifically, I can design targeting and validation strategies for the creation of mutant mice and other organisms (happy to work on non-model organisms), for example, a gene knockout by introducing a stop codon, an N-terminus GFP fusion, or the introduction of a short mutation like an SNP or HA tag. I have 8 years of experience in this area and have produced three successful modified mice for experiments and validated projects. Given a desired model organism, I can plan the experiment and give you step-by-step instructions to complete the molecular biology components in your lab and advise on which companies to buy reagents which are ideal for the working processes. I am also an expert on NGS, particularly all aspect of the Ion Torrent sequencing platform, from sample QC and library construction to operating the instruments, assessing runs, and interpreting the results. I am also knowledgeable about PacBio and Illumina platforms and can provide consulting about experimental design, library prep, and results. Software: Benchling (for Sanger sequencing analysis, CRISPR targeting designs, PCR and qPCR primer design), Ion Reporter for Ion Torrent sequencing, Galaxy, R/RStudio, TrinityRNAseq, limma for RNAseq analysis. I have previously successfully completed the following Projects: (1) Mechanisms of Immune impaired in chronic Hepatitis C Virus infection. (2) Gut hormones leptin and ghrelin: Proteomics and Genomics approach for studying H. pylori positive related gastric cancer: Facilitate the development of new therapy for Immunocompetence (In collaboration with Nobel laureate Prof. BarryMarshall). My current position would be very much in use for your side as a good and resourceful researcher.
• Ob 6.1 clone for the obesity-related PCOS study - Using RTcPCR. (Through Prof. Hubert Vidal DIRECTOR, INSERM U 449, I have obtained ob 6.1 clone for the obesity-related PCOS study).
• Micro RNA (17.3P.5) gene expression study in YPEN and U87 breast cancer cell line and in control PNI cell line - Using qPCR
• Endocytic Function of von Hippel-Lindau Tumor Suppressor Protein Regulates Surface Localization of Fibroblast Growth Factor Receptor 1 and Cell Motility – Using Cell-surface Biotinylation Assay
• “Studying the host factors associated with age-related female infertility” & “Identifying and suppressing the ovarian miRNAs overexpressed in polycystic ovarian syndrome disease” – Using Micro Array Techniques.
• Mechanisms of Immune impaired in chronic Hepatitis C Virus infection – Using Flow cytometer.
• Proteomics and Genomics approach for studying obesity-related gastric cancer: Facilitate the development of new therapy for Immunocompetence - Agilent 6540 UHD Accurate-Mass Q-TOF mass spectrometer coupled with Agilent 1290 or Agilent 1260 High Performance Liquid Chromatography separation system - Metabolomics & Proteomics.
• Polymorphisms in host CYP2C19 gene and antibiotic resistance attributes of Helicobacter pylori isolates influence the outcome of triple therapy & Polymorphisms at Locus 4p14 of Toll-Like Receptors TLR-1 and TLR-10 Confer Susceptibility to Gastric Carcinoma in Helicobacter pylori Infection by ddPCR.
• Laboratory of Early Mammalian Developmental Biology (LEMDB)
o To definitively identify the exact p38-Mapk paralogue(s) (of the four presents in the mouse genome) responsible for the published observations on PrE differentiation (based on selective pharmacological inhibition of p38-Mapk activity1). Moreover, to ascertain if p38-Mapk activation can be achieved by signaling pathways other than those mediated by FGF-signalling. We employ classical embryological and molecular biological techniques to probe the mechanisms of action of candidate cell fate influencing genes, identified in genomic scale expression screens of the mouse embryo and mouse ES cells. Hence, we anticipate that our multi-disciplined approach, investigating the ‘in vivo’ balance between cell differentiation and retention of pluripotency in early mouse embryogenesis, will not only advance our fundamental knowledge of one of the most enigmatic periods of development but will also provide insight to clinical/ veterinary embryologists and those clinicians working in the field of regenerative stem cell-based medicine.
• SPECIES DIVERSITY OF RATS IN OIL PALM PLANTATIONS IN PENINSULAR MALAYSIA AND ITS CONTROL STRATEGIES
My working techniques and my dedication to delivering results will be also a plus point for the research projects that are given to me. I am trained in many different types of techniques and willing to learn more about the latest techniques to improve and to deliver the best in me for any situation.


Primary Affiliation: University of Malaya - Kuala Lumpur, Kuala Lumpur , Malaysia

Specialties:

Additional Specialties:

Research Interests:


View Dr Ravishankar Ram Mani’s Resume / CV

Education

May 2006
Madras University
PhD
Biomedical Genetics

Experience

Apr 2019
SPECIES DIVERSITY OF RATS IN OIL PALM PLANTATIONS IN PENINSULAR MALAYSIA AND ITS CONTROL STRATEGIES
Senior Research Fellow
Aug 2018
Research Scientist (Research Track) | Department of Molecular biology
Senior Researcher
understanding the molecular mechanisms that underpin mammalian preimplantation embryo development; specifically, the derivation of the first three cell lineages (trophectoderm, primitive endoderm
Jul 2018
Proteomics and Genomics approaches for studying obesity related gastric cancer: Facilitate the development of new therapy for Immunocompetence, and investigation of regulatory metabolites and protein analysis by Metabolomics
Senior Researcher

Publications

14Publications

408Reads

6Profile Views

3PubMed Central Citations

Expression of Leptin (Ob Gene Product) in Reproductive System with Special Reference to Polycystic Ovary Syndrome

Int J Health Res, September 2010; 3(3): 1 65

International Journal of Health Research

Purpose: To determine serum leptin and its ob mRNA expression both in the PCOS and non-PCOS ovary, endometrium and adipose tissue in normal or polycystic ovary syndrome (PCOS) in South Indian population. Methods: The regulation of ob gene expression in thin, overweight, obese and morbidly obese PCOS (Polycystic Ovary Syndrome) and non-PCOS subject’s endometrium, ovary and adipose tissue were investigated using a reverse transcription-competitive polymerase chain reaction method to quantify the mRNA levels of leptin and compared with normal weight control adipose tissue. Results: Endometrium, ovary and adipose tissue ob mRNA levels were highly correlated with serum leptin, BMI and body fat distribution of 80 subjects (10 normal weight, 8 thin PCOS, 8 thin non-PCOS, 7 overweight PCOS, 7 overweight non- PCOS, 10 obese PCOS, 10 obese non-PCOS, 10 morbidly obese PCOS and 10 morbidly obese non-PCOS). Ob mRNA levels were positively correlated with serum levels of dehydroepiandrosterone sulphate, free testosterone, luteinizing hormone, and prolactin. Ob mRNA level was inversely correlated with SHBG and androstenedione. Conclusion: Here, we report for the first time in Indian population, that endometrium, ovary, adipose tissue ob mRNA levels and serum leptin are highly correlated and are different in expression levels in thin, obese, morbidly obese PCOS and non-PCOS, when compared to individuals with normal weight. The leptin could be a novel, independent risk factor for PCOS.

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October 2018
3 Reads

Giant Oyster Mushroom Pleurotus giganteus (Agaricomycetes) Enhances Adipocyte Differentiation and Glucose Uptake via Activation of PPARγ and Glucose Transporters 1 and 4 in 3T3-L1 Cells.

Int J Med Mushrooms 2016 ;18(9):821-831

Mushroom Research Centre (MRC), Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

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http://www.dl.begellhouse.com/journals/708ae68d64b17c52,2e89
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http://dx.doi.org/10.1615/IntJMedMushrooms.v18.i9.60DOI Listing
March 2017
21 Reads

CD8+ T cells of chronic HCV-infected patients express multiple negative immune checkpoints following stimulation with HCV peptides.

Cell Immunol 2017 03 16;313:1-9. Epub 2016 Dec 16.

Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia; Division of Infection Biology, Department of Life Sciences, School of Basic and Applied Sciences, Central University of Tamil Nadu (CUTN), Thiruvarur 610 005, India; Center of Excellence for Research in AIDS (CERiA), University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia. Electronic address:

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http://dx.doi.org/10.1016/j.cellimm.2016.12.002DOI Listing
March 2017
26 Reads
3 Citations
1.924 Impact Factor

Pleurotus giganteus (Berk. Karun & Hyde), the giant oyster mushroom inhibits NO production in LPS/HO stimulated RAW 264.7 cells via STAT 3 and COX-2 pathways.

BMC Complement Altern Med 2017 Jan 13;17(1):40. Epub 2017 Jan 13.

Mushroom Research Centre, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.

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http://dx.doi.org/10.1186/s12906-016-1546-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5237140PMC
January 2017
58 Reads
2.020 Impact Factor

Polymorphisms at Locus 4p14 of Toll-Like Receptors TLR-1 and TLR-10 Confer Susceptibility to Gastric Carcinoma in Helicobacter pylori Infection.

PLoS One 2015 11;10(11):e0141865. Epub 2015 Nov 11.

Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Lembah Pantai, 50603, Kuala Lumpur, Malaysia.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0141865PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4641589PMC
June 2016
116 Reads
3.234 Impact Factor

Monoterpenes: Novel insights into their biological effects and roles on glucose uptake and lipid metabolism in 3T3-L1 adipocytes.

Food Chem 2016 Apr 12;196:242-50. Epub 2015 Sep 12.

Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address:

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http://dx.doi.org/10.1016/j.foodchem.2015.09.042DOI Listing
April 2016
6 Reads
3.391 Impact Factor

Peripheral loss of CD8(+) CD161(++) TCRVα7·2(+) mucosal-associated invariant T cells in chronic hepatitis C virus-infected patients.

Eur J Clin Invest 2016 Feb 5;46(2):170-80. Epub 2016 Jan 5.

Tropical Infectious Disease Research and Education Center (TIDREC), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

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http://dx.doi.org/10.1111/eci.12581DOI Listing
February 2016
30 Reads
2.734 Impact Factor

Increased frequency of late-senescent T cells lacking CD127 in chronic hepatitis C disease.

Eur J Clin Invest 2015 May 16;45(5):466-74. Epub 2015 Mar 16.

Department of Medical Microbiology, Tropical Infectious Disease Research and Education Center (TIDREC), University of Malaya, Lembah Pantai, Kuala Lumpur, Malaysia.

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http://dx.doi.org/10.1111/eci.12429DOI Listing
May 2015
15 Reads
2.734 Impact Factor

Analysis of structure, function, and evolutionary origin of the ob gene product--leptin.

J Biomol Struct Dyn 2007 Oct;25(2):183-8

Department of Genetics, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600 113, India.

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http://dx.doi.org/10.1080/07391102.2007.10507167DOI Listing
October 2007
11 Reads
2.983 Impact Factor

Possible correlation of leptin with body fat distribution and adiposity: Evaluation of serum leptin in South Indian population.

Reprod Med Biol 2007 Jun 14;6(2):117-125. Epub 2007 May 14.

Department of Genetics, Dr ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, Tamilnadu, India.

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http://dx.doi.org/10.1111/j.1447-0578.2007.00174.xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904587PMC
June 2007
7 Reads

Cytokines and leptin correlation in patients with polycystic ovary syndrome: Biochemical evaluation in south Indian population.

Reprod Med Biol 2005 Dec 2;4(4):247-254. Epub 2005 Nov 2.

Department of Genetics, Dr ALM PG Institute of Basic Medical sciences, University of Madras, Taramani.

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http://dx.doi.org/10.1111/j.1447-0578.2005.00114.xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904660PMC
December 2005
9 Reads

Body fat distribution and leptin correlation in women with polycystic ovary syndrome: Endocrine and biochemical evaluation in south Indian population.

Reprod Med Biol 2005 Mar 7;4(1):71-78. Epub 2005 Mar 7.

Department of Genetics, Dr ALM PG Institute of Basic Medical sciences, University of Madras, Taramani, Chennai.

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http://dx.doi.org/10.1007/bf03016140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904616PMC
March 2005
17 Reads