Publications by authors named "Man Zhang"

620 Publications

The bZIP transcription factor GmbZIP15 facilitates resistance against and infection in soybean.

iScience 2021 Jun 24;24(6):102642. Epub 2021 May 24.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Plant Protection, College of Life Sciences, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China.

Soybean, one of the most valuable oilseed crops, is under constant pressure from pathogens. bZIP transcription factors (TFs) composing one of the largest TF families in plants have diverse functions. Biochemical and physiological analyses were performed to characterize the regulatory roles of soybean bZIP TF GmbZIP15 in response to pathogens. We found that transgenic soybean plants overexpressing has increased resistance against and . Besides, regulates pathogen response by modulating the antioxidant defense system and phytohormone signaling. In addition, we performed chromatin immunoprecipitation sequencing to identify the downstream genes of in response to . and found that can activate or repress the expression of defense-related genes through direct promoter binding. Taken together, these results indicate that plays a positive role in pathogen resistance in soybean, and this activity may be dependent on phytohormone signaling.
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http://dx.doi.org/10.1016/j.isci.2021.102642DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188564PMC
June 2021

Hydraulic performance prediction and optimization of an engine cooling water pump using computational fluid dynamic analysis.

PLoS One 2021 15;16(6):e0253309. Epub 2021 Jun 15.

College of Mechanical and Electrical Engineering, Shaanxi University of Science and Technology, Xi'an, Shaanxi, China.

In current research, the hydraulic performance prediction and optimization of an engine cooling water pump was conducted by computational fluid dynamic (CFD) analysis. Through CFD simulation, the pump head, shaft power and efficiency for the original pump at volume flow rate 25 L/min and impeller rotating speed 4231 r/min were 3.87 m, 66.7 W and 23.09% respectively. For improving hydraulic performance, an optimization study was carried out. After optimization, four potential optimized designs were put forward. The efficiency of the optimized design No.1 for engine cooling water pump was nearly 6% higher than that of the original pump model; and the head of the optimized design No.2 for engine cooling water pump was 9% higher than that of the original pump model. Under the condition of maintaining the pump head and considering comprehensive improvement effect, the optimized design No.3 was considered as the best design and selected as the test case for validating the optimum design. The hydraulic performance predictions for this optimum engine cooling water pump agreed well with experimental data at design condition with relative discrepancies of 2.9% and 5.5% for the pump head and pump efficiency, respectively. It proved that performance prediction calculation model and the automatic optimization model were effective. This research work can provide theoretical basis for the design, development and optimization of engine cooling water pump.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253309PLOS
June 2021

A 'Concentrate-&-Destroy' technology for enhanced removal and destruction of per- and polyfluoroalkyl substances in municipal landfill leachate.

Sci Total Environ 2021 May 29;791:148124. Epub 2021 May 29.

CTI and Associates, Inc., 28001 Cabot Dr #250, Novi, MI 48377, USA.

Per- and polyfluoroalkyl substances (PFAS) are ubiquitous in landfill leachate due to their widespread applications in various industrial and consumer products. Yet, there has been no cost-effective technology available for treating PFAS in leachate because of the intrinsic persistency of PFAS and the high matrix strength of landfill leachate. We tested a two-step 'Concentrate-&-Destroy' technology for treating over 14 PFAS from a model landfill leachate through bench- and pilot-scale experiments. The technology was based on an adsorptive photocatalyst (Fe/[email protected]), which was able to selectively adsorb PFAS despite the strong matrix effect of the leachate. Moreover, the pre-concentrated PFAS on Fe/[email protected] were effectively degraded under UV, which also regenerates the material. The presence of 0.5 M HO during the photocatalytic degradation enhanced the solid-phase destruction of the PFAS. Fresh Fe/[email protected] at a dosage of 10 g/L removed >95% of 13 PFAS from the leachate, 86% after first regeneration, and 74% when reused three times. Fe/[email protected] was less effective for PFBA and PFPeA partially due to the transformation of precursors and/or longer-chain homologues into these short-chain PFAS. Pilot-scale tests preliminarily confirmed the bench-scale results. Despite the strong interference from additional suspended solids, Fe/[email protected] removed >92% of 18 PFAS in 8 h under the field conditions, and when the PFAS-laden solids were subjected to the UV-HO system, ~84% of 16 PFAS in the solid phase were degraded. The 'Concentrate-&-Destroy' strategy appears promising for more cost-effective removal and degradation of PFAS in landfill leachate or PFAS-laden high-strength wastewaters.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148124DOI Listing
May 2021

Termitomenins F and G, Two New Lignan Glucosides from Terminalia chebula var. tomentella (Kurz) C. B. Clarke.

Nat Prod Bioprospect 2021 Jun 10. Epub 2021 Jun 10.

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China.

The extensive chemical investigation on the branches and leaves of Terminalia chebula var. tomentella (Combretaceae) led to the isolation of two new lignan glucosides with a furofuran skeleton, termitomenins F (1) and G (2). In addition, 19 known compounds including five lignan glucosides (3-7), six hydrolyzable tannins (8-13) and eight simple phenolics (14-21) were also identified. Their structures were determined by comprehensive spectroscopic analyses. It is noted that 8 and 9 were C-glycosidic hydrolyzable tannins with one hexahydroxydiphenoyl and one gallagyl group linked to an open-chain glucosyl C-1/O-2/O-3 and O-4/O-6, respectively, which were rarely found in plants. Nine known compounds, 6-9, 13, and 18-21, were procured from the titled plant for the first time, while 3-5, 10-12 and 14-17 were also found in the fruits. Notably, the known hydrolyzable tannins 8-13 exhibited stronger α-glucosidase inhibitory activities with IC values ranging from 0.10 to 3.12 μM, than the positive control, quercetin (IC = 9.38 ± 0.33 μM).
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http://dx.doi.org/10.1007/s13659-021-00314-zDOI Listing
June 2021

Interaction of the Exopolysaccharide from YW11 with Casein and Bioactivities of the Polymer Complex.

Foods 2021 May 21;10(6). Epub 2021 May 21.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China.

There has been an increased application of exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) in fermented dairy products, but interactions between EPS and casein (CAS), and bioactivities of their complex are poorly studied. In this study, EPS produced by YW11 (EPS-YW11) was studied for interactions with CAS in a simulated fermentation system acidified by D-(+)-gluconic acid δ-lactone. The results showed that there was interaction between EPS-YW11 and CAS when EPS (up to 1%, /) was added to the casein solution (3%, /) as observed with increased viscoelasticity, water holding capacity, ζ-potential and particle size of EPS-YW11/CAS complex compared with CAS alone. Microstructural analysis showed that a higher concentration of EPS facilitated more even distribution of CAS particles that were connected through the polysaccharide chains. Infrared spectroscopy further confirmed interactions between EPS and CAS by intermolecular hydrogen bonding, electrostatic and hydrophobic contacts. Further evaluation of the bioactivities of EPS-YW11/CAS complex revealed significantly increased antibiofilm, antioxidation, and bile acids binding capacity. The present study provides further understanding on the mechanism of interactions between EPS produced by LAB and CAS, which would benefit potential applications of EPS in fermented dairy products with enhanced functionality.
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http://dx.doi.org/10.3390/foods10061153DOI Listing
May 2021

Effect of Mobile-Based Lifestyle Intervention on Body Weight, Glucose and Lipid Metabolism among the Overweight and Obese Elderly Population in China: A Randomized Controlled Trial Protocol.

Int J Environ Res Public Health 2021 05 1;18(9). Epub 2021 May 1.

Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.

: Promotion of a healthy lifestyle is considered a good strategy for dealing with chronic diseases. Mobile-based lifestyle interventions have shown beneficial effects in the control and treatment of chronic diseases such as diabetes, obesity and metabolic syndrome. Current clinical trials for mobile-based lifestyle intervention were mainly conducted among non-elderly populations, thus well-designed trials performed among the elderly who are more susceptible to chronic diseases are needed. The study aims to assess the effect of the mobile-based lifestyle intervention on the improvement of body weight, glucose and lipid metabolism among overweight and obese elderly adults in China. : Participants aged 60-80 years who are overweight or obese will be randomly assigned to receive mobile-based nutrition and exercise intervention, mobile-based exercise intervention and no intervention for 3 months. Before the intervention, participants will receive the training of the mobile application and sports bracelet. The primary outcome will be the between-group (three groups) difference in body mass index at the end of intervention. The secondary outcomes will include body composition, parameters of glucose and lipid metabolism, blood pressure, dietary data and physical activity data. All these outcomes will be assessed at baseline, day 45 and day 90. : The trial has been approved by the Ethics Committee of Peking University Health Science Center (IRB00001052-18039).
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http://dx.doi.org/10.3390/ijerph18094854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125256PMC
May 2021

Identification of the PmWEEP locus controlling weeping traits in Prunus mume through an integrated genome-wide association study and quantitative trait locus mapping.

Hortic Res 2021 Jun 1;8(1):131. Epub 2021 Jun 1.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083, Beijing, China.

Weeping Prunus mume (mei) has long been cultivated in East Asia for its specific ornamental value. However, little is known about the regulatory mechanism of the weeping trait in mei, which limits molecular breeding for the improvement of weeping-type cultivars. Here, we quantified the weeping trait in mei using nested phenotyping of 214 accessions and 342 F hybrids. Two major associated loci were identified from the genome-wide association study (GWAS), which was conducted using 3,014,409 single nucleotide polymorphisms (SNPs) derived from resequencing, and 8 QTLs and 55 epistatic loci were identified from QTL mapping using 7,545 specific lengths amplified fragment (SLAF) markers. Notably, an overlapping PmWEEP major QTL was fine mapped within a 0.29 Mb region on chromosome 7 (Pa7), and a core SNP locus closely associated with the weeping trait was screened and validated. Furthermore, a total of 22 genes in the PmWEEP QTL region were expressed in weeping or upright mei based on RNA-seq analysis. Among them, only a novel gene (Pm024213) containing a thioredoxin (Trx) domain was found to be close to the core SNP and specifically expressed in buds and branches of weeping mei. Co-expression analysis of Pm024213 showed that most of the related genes were involved in auxin and lignin biosynthesis. These findings provide insights into the regulatory mechanism of the weeping trait and effective molecular markers for molecular-assisted breeding in Prunus mume.
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http://dx.doi.org/10.1038/s41438-021-00573-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167129PMC
June 2021

Biomechanical Cervical Assessment Using 2-Dimentional Transvaginal Shear Wave Elastography in Nonpregnant and Pregnant Women: A Prospective Pilot Study.

Ultrasound Q 2021 Jun;37(2):183-190

Obstetrics and Gynecology.

Abstract: This study evaluated the technical feasibility of 2-dimensional transvaginal shear wave elastography to quantify cervical stiffness in nonpregnant and pregnant women and established normal values in each group. With institutional review board approval, we performed a prospective study with an age-matched historical control design. Sixteen premenopausal nonpregnant women without cervical pathology and 17 low-risk pregnant women (gestational age 17-33 weeks) were enrolled. Cervical shear wave speeds were measured on a SuperSonic Aixplorer machine. The mean shear wave speeds of anterior cervix were 4.96 ± 1.96 m/s in nonpregnant women and 1.92 ± 0.31 m/s in pregnant women. No significant stiffness difference was found between the anterior and posterior cervix (P = 0.15). The upper cervix was stiffer than the lower cervix in the pregnant women (P = 0.00012). Transvaginal shear wave elastography reveals that cervix at a midterm gestation is significantly softer than nonpregnant cervix (P < 0.0001) and suggests a spatial stiffness gradient along the length of the cervix, consistent with histopathology and limited elastography literature. Our results indicate the potential of transvaginal shear wave elastography to provide objective and quantitative estimates of cervical stiffness, especially during pregnancy.
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http://dx.doi.org/10.1097/RUQ.0000000000000561DOI Listing
June 2021

The Effect of Perceived Overqualification on Creative Performance: Person-Organization Fit Perspective.

Front Psychol 2021 13;12:582367. Epub 2021 May 13.

School of Management, Shanghai University, Shanghai, China.

In today's business world, the phenomenon of overqualification is widespread. Organizations need to consider - how to motivate the overqualified employees to utilize their qualifications, for example, promoting creative performance. Based on person-organization (P-O) fit theory, this study explored when and how employees, who feel overqualified can engage in creative performance. Data were collected from 170 supervisor-subordinate dyads of 41 groups in 10 manufacturing companies across two timepoints in China. Results revealed that (1) perceived overqualification is positively related to organizational identification when employee development-oriented organizational culture is strong but negatively related to organizational identification when employee development-oriented organizational culture is weak, (2) organizational identification is positively related to creative performance, (3) the indirect relationship between perceived overqualification and creative performance via organizational identification is moderated by employee development-oriented organizational culture. We provide several theoretical contributions to the overqualification literature and make some suggestions to motivate the overqualified employees to use their surplus qualifications within the organizations.
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http://dx.doi.org/10.3389/fpsyg.2021.582367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155303PMC
May 2021

Dynamic changes in mitochondrial DNA, morphology, and fission during oogenesis of a seasonal-breeding teleost, Pampus argenteus.

Tissue Cell 2021 May 21;72:101558. Epub 2021 May 21.

College of Marine Science, Ningbo University, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo, China; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China.

Mitochondria play crucial roles during oocyte development. In this study, we have investigated mitochondrial morphology, mtDNA, Ca-ATP enzyme activity, and mitochondrial fission factor (mff) expression levels during oogenesis of the silver pomfret Pampus argenteus. The mtDNA increased with oocyte development, and mitochondrial morphology and distribution were stage-specific. In the perinucleolar oocytes, oval mitochondria were dispersed in the cytoplasm. In previtellogenic oocytes, mitochondria massively increased and aggregated, forming mitochondrial clouds. At the same time, two morphologically different types of mitochondria had been distinguished, one of which was elongated with well-developed cristae, and the other was round with distorted and fused cristae. During vitellogenesis, the increases in mitochondria with well-developed cristae and in Ca-ATPase enzymatic activity were accompanied by an accumulation of yolk substance, suggesting the possible participation of mitochondria in the formation of vitellogenesis. Furthermore, we examined the cDNA of mff its transcript levels in relation to oocyte development. The transcript levels of mff were high in the perinucleolar stage, increasing to the highest level at the previtellogenic stage. Immunocytochemistry showed that MFF was detected in the cytoplasm of previtellogenic and midvitellogenic oocytes. We speculated that the mff-mediated mitochondrial fission may play a crucial role in oocyte development, especially in vitellogenesis.
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http://dx.doi.org/10.1016/j.tice.2021.101558DOI Listing
May 2021

Establishment of clinical diagnostic models using glucose, lipid, and urinary polypeptides in gestational diabetes mellitus.

J Clin Lab Anal 2021 May 27:e23833. Epub 2021 May 27.

Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Background: Gestational diabetes mellitus (GDM) has many adverse outcomes that seriously threaten the short-term and long-term health of mothers and infants. This study comprehensively analyzed the clinical diagnostic value of GDM-related clinical indexes and urine polypeptide research results, and established comprehensive index diagnostic models.

Methods: In this study, diagnostic values from the clinical indexes of serum triglyceride (TRIG), high-density lipoprotein cholesterol (HDL-C), fasting plasma glucose (FPG) and glycosylated hemoglobin (HbA1c), and 7 GDM-related urinary polypeptides were analyzed retrospectively. The multiple logistic regression equation, multilayer perceptron neural network model, radial basis function, and discriminant analysis function models of GDM-related indexes were established using machine language.

Results: The results showed that HbA1c had the highest diagnostic value for GDM, with an area under the curve (AUC) of 0.769. When the cut-off value was 4.95, the diagnostic sensitivity and specificity were 70.5% and 70.0%, respectively. Among the seven GDM-related urinary polypeptides, human hemopexin (HEMO) had the highest diagnostic value, with an AUC of 0.690. When the cut-off value was 368.5, the sensitivity and specificity were 79.5% and 43.3%, respectively. The AUC of the multilayer perceptron neural network model was 0.942, followed by binary logistic regression (0.938), radial basis function model (0.909), and the discriminant analysis function model (0.908).

Conclusion: The establishment of a GDM diagnostic model combining blood glucose, blood lipid, and urine polypeptide indexes can lay a foundation for exploring machine language and artificial intelligence in diagnostic systems.
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http://dx.doi.org/10.1002/jcla.23833DOI Listing
May 2021

Insights into the relationships between physicochemical properties, solvent performance, and applications of deep eutectic solvents.

Environ Sci Pollut Res Int 2021 May 24. Epub 2021 May 24.

School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.

Deep eutectic solvent (DES) is regarded as a new generation of green solvent due to its distinctive and tailorable physicochemical properties, such as low volatility, strong solubility, biodegradability, low-cost, environment-friendly, and feasibility of the structural design. As an alternative to traditional organic solvents and ionic liquids (ILs), DESs have been widely applied in many fields, such as organic chemical synthesis, electrochemical deposition, material preparation, biomass catalytic conversion, extraction and separation, detection and analysis, nanotechnology, gas absorption, and drug delivery. In this paper, through in-depth discussion on factors influencing the physicochemical properties of DESs, we summarized the relations between their composition, structure, and performance. Focusing on their solvent performance, we analyzed the latest research results of DESs with different physicochemical properties in various fields. It should be pointed out that designing and synthesizing DESs from the molecular structure aspect to regulate their physicochemical properties is the direction of accurately developing new functional applications of DESs.
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http://dx.doi.org/10.1007/s11356-021-14485-2DOI Listing
May 2021

Artificial intelligence models derived from 2D transperineal ultrasound images in the clinical diagnosis of stress urinary incontinence.

Int Urogynecol J 2021 May 24. Epub 2021 May 24.

Department of Ultrasound, The Third Affiliated Hospital of Sun Yat-Sen University, NO.600 Tianhe Road, Guangzhou, 510630, Guangdong Province, China.

Introduction And Hypothesis: The aim of the study was to develop artificial intelligence (AI) algorithms using 2D transperineal ultrasound (TPUS) static images to simplify the clinical process of diagnosing stress urinary incontinence (SUI) in practice.

Methods: The study involved 400 patients in total, including 265 SUI patients and 135 non-SUI patients who underwent a routine clinical evaluation process by urologists and TPUS. They were classified into different groups based on the International Consultation on Incontinence Questionnaire (ICIQ) to assess the impact of inconvenience on patients' lives. Four AI models were developed by 2D TPUS images: Model A (a single-mode model based on Valsalva maneuver images to classify G-0, G-1, and G-2); Model B (a dual-mode model based on Valsalva maneuver and resting state images to classify G-0, G-1, and G-2); Model C (a single-mode model based on Valsalva maneuver images to classify G-2 and G-01); Model D (a dual-mode model based on Valsalva maneuver and resting state images to classify G-2 and G-01). The performance of the four models was evaluated by confusion matrices and the area under the receiver-operating characteristic curve (AUC).

Results: The dual-mode model based on the Valsalva maneuver and resting-state images (Model D) had a higher accuracy of 86.3% and an AUC of 0.922, which was significantly higher than the AUCs of the other three models: 0.771, 0.862, and 0.827.

Conclusions: The AI algorithm using 2D TPUS static images of the Valsalva maneuver and resting state may be a promising tool in the diagnosis of SUI patients in to relieve clinical processes in practice given its ease of use in clinical applications.
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http://dx.doi.org/10.1007/s00192-021-04859-yDOI Listing
May 2021

Evaluation of the bioaccessibility of tetrahydrocurcumin-hyaluronic acid conjugate using in vitro and ex vivo models.

Int J Biol Macromol 2021 May 15;182:1322-1330. Epub 2021 May 15.

Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA. Electronic address:

Tetrahydrocurcumin-hyaluronic acid (THC-HA) conjugate was synthesized in order to improve the bioaccessibility of tetrahydrocurcumin (THC). The successful conjugation was confirmed by the results from H-nuclear magnetic resonance (H NMR), Differential scanning calorimetry (DSC) and X-ray diffraction (XRD). Bioaccessibility enhancement from the THC-HA conjugate compared to the free crystalline THC suspension was demonstrated by the results from ex vivo Franz diffusion cell using small intestine from porcine and in vitro TNO dynamic gastrointestinal model-1 (TIM-1). Additionally, in vitro release was studied, and the integrity of the conjugate in both simulated gastric and intestinal conditions was found to maintain for up to 4 h. Mucoadhesive assay and rheological results indicated that the mucoadhesive property of THC-HA, in combination with the aqueous solubility enhancement, might contribute to the increased bioaccessibility. This study provides a promising approach to enhance the bioaccessibility of tetrahydrocurcumin through the innovative conjugation with hyaluronic acid.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.086DOI Listing
May 2021

Measuring glucose concentration in a solution based on the indices of polarimetric purity.

Biomed Opt Express 2021 Apr 29;12(4):2447-2459. Epub 2021 Mar 29.

School of Computer and Information, Hefei University of Technology, Hefei, 230009, China.

Polarization imaging is a powerful tool, which can be applied in biomedical diagnosis and many research fields. Here, we propose a new application of the indices of polarimetric purity (IPPs) composed of P1, P2, P3, to describe the glucose concentrations (GC) changes in the scattering system. The results suggest that P1 of the IPPs is a better indicator to GC in the solution than the degree of polarization (DoP) for the forward scattering detection. Meanwhile, the fitting relation among radius of scattering particle, GCs and P1 parameter has also been calculated, in which the error of inversion is no more than 4.73%. In the backscattering detection, the fitted frequency statistical histogram of the IPPs is used to measure the GCs, and their modes can represent changing trend of GCs.
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http://dx.doi.org/10.1364/BOE.414850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086474PMC
April 2021

Targeting LIMK1 with luteolin inhibits the growth of lung cancer in vitro and in vivo.

J Cell Mol Med 2021 Jun 13;25(12):5560-5571. Epub 2021 May 13.

China-US (Henan) Hormel Cancer Institute, Zhengzhou, China.

Lung cancer is the leading cause of cancer-related deaths. LIM domain kinase (LIMK) 1 is a member of serine/threonine kinase family and highly expressed in various cancers. Luteolin, a polyphenolic plant flavonoid, has been reported to suppress tumour proliferation through inducing apoptosis and autophagy via MAPK activation in glioma. However, the mechanism of luteolin on suppressing lung cancer growth is still unclear. We found that luteolin targeted LIMK1 from the in silico screening and significantly inhibited the LIMK1 kinase activity, which was confirmed with pull-down binding assay and computational docking models. Treatment with luteolin inhibited lung cancer cells anchorage-independent colony growth and induced apoptosis and cell cycle arrest at G1 phase. Luteolin also decreased the expression of cyclin D1 and increased the levels of cleaved caspase-3 by down-regulating LIMK1 signalling related targets, including p-LIMK and p-cofilin. Furthermore, luteolin suppressed the lung cancer patient-derived xenograft tumour growth by decreasing Ki-67, p-LIMK and p-cofilin expression in vivo. Taken together, these results provide insight into the mechanism that underlies the anticancer effects of luteolin on lung cancer, which involved in down-regulation of LIMK1 and its interaction with cofilin. It also provides valuable evidence for translation towards lung cancer clinical trials with luteolin.
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http://dx.doi.org/10.1111/jcmm.16568DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184676PMC
June 2021

Ni-Catalyzed radical cyclization of vinyl azides with cyclobutanone oxime esters to access cyanoalkyl containing quinoxalin-2(1)-ones.

Org Biomol Chem 2021 Jun;19(21):4697-4700

Key Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Key Laboratory of Molecule-Based Materials, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, Anhui 241000, China.

A nickel-catalyzed cascade addition/cyclization of 2-azido-N-arylacrylamides and cyclobutanone oxime esters for the construction of 3-cyanoalkylated quinoxalin-2(1H)-ones is developed. This reaction proceeds under mild conditions with good functional group tolerance and broad substrate scope. A preliminary mechanistic experiment indicated that the cyanoalkyl radical might be involved in this transformation.
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http://dx.doi.org/10.1039/d1ob00610jDOI Listing
June 2021

Single-cell analysis reveals urothelial cell heterogeneity and regenerative cues following cyclophosphamide-induced bladder injury.

Cell Death Dis 2021 May 5;12(5):446. Epub 2021 May 5.

State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.

Cyclophosphamide is a commonly used chemotherapeutic drug to treat cancer with side effects that trigger bladder injury and hemorrhagic cystitis. Although previous studies have demonstrated that certain cell subsets and communications are activated to drive the repair and regeneration of bladder, it is not well understood how distinct bladder cell subsets function synergistically in this process. Here, we used droplet-based single-cell RNA sequencing (scRNA-seq) to profile the cell types within the murine bladder mucous layer under normal and injured conditions. Our analysis showed that superficial cells are directly repaired by cycling intermediate cells. We further identified two resident mesenchymal lineages (Acta2 myofibroblasts and Cd34 fibroblasts). The delineation of cell-cell communications revealed that Acta2 myofibroblasts upregulated Fgf7 expression during acute injury, which activated Fgfr signaling in progenitor cells within the basal/intermediate layers to promote urothelial cell growth and repair. Overall, our study contributes to a more comprehensive understanding of the cellular dynamics during cyclophosphamide-induced bladder injury and may help identify important niche factors contributing to the regeneration of injured bladders.
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http://dx.doi.org/10.1038/s41419-021-03740-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099875PMC
May 2021

Clinical value for the detection of fetal chromosomal deletions/duplications by noninvasive prenatal testing in clinical practice.

Mol Genet Genomic Med 2021 May 5:e1687. Epub 2021 May 5.

Center for Genetic Medicine, Xuzhou Maternity and Child Health Care Hospital Affiliated to Xuzhou Medical University, Xuzhou, China.

Objective: This study was to report the experiences on the clinical value of noninvasive prenatal testing (NIPT) for the screening of fetal chromosomal deletions/duplications.

Methods: We performed a retrospective analysis of a cohort of 20,439 pregnancies undergoing NIPT from March 2017 to September 2020 at a single center. Patients with positive NIPT results for fetal chromosomal deletions or duplications had options of invasive diagnostic testing or no further testing. The data were complied from all cases with positive NIPT results for chromosomal deletions/duplications. The positive predictive value (PPV) was calculated from tabulated data.

Results: In this cohort, positive NIPT results for fetal chromosomal deletions/duplications were found in 60 pregnant women. Of the positive samples, further invasive testing was performed in 39 cases, in which 9 cases were found to be true positive. The overall PPV for chromosomal deletions/duplications was 23.1%. In addition, fetal structural anomaly was found by ultrasound examination in three cases, in which the chromosomal deletions/duplications of three cases were not verified. Moreover, an unexpected pathogenic 8p23.3 deletion was identified by invasive testing in 1 fetus with a false positive NIPT screen for 3q27.3q29 duplication.

Conclusions: In summary, positive NIPT results of chromosomal deletions/duplications were not uncommon in clinical practice, whereas the PPV for the testing was low. Hence, potential risks and high percentage of false positives for these abnormal NIPT results might be informed to pregnant women before the choice made of invasive testing.
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http://dx.doi.org/10.1002/mgg3.1687DOI Listing
May 2021

Development of active and smart packaging films based on starch, polyvinyl alcohol and betacyanins from different plant sources.

Int J Biol Macromol 2021 Apr 28;183:358-368. Epub 2021 Apr 28.

College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, PR China. Electronic address:

In this study, active and smart packaging films were prepared by individually adding betacyanins-rich plant extracts (red pitaya flesh extract (RPFE), prickly pear fruit extract (PPFE), red beetroot extract (RBRE), globe amaranth flower extract (GAFE) and red amaranth leaf extract (RALE)) into starch/polyvinyl alcohol. The structural, physical and functional properties of the films containing betacyanins from different plant sources were compared for the first time. Results showed betacyanins from RPFE, PPFE and RBRE were betanin-type betacyanins. Betacyanins from GAFE and RALE were gomphrenin-type and amaranthin-type betacyanins, respectively. The films containing RPFE and PPFE presented more uniform cross-sections and had the highest water vapor barrier (9.37 and 9.26 × 10 g m s Pa) and mechanical strength (8.26 and 7.87 MPa). However, the film containing GAFE presented the lowest light transmittance but the highest 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (EC = 0.47 mg/mL). All the films containing betacyanins are sensitive to pH 8-12 buffer solutions and volatile ammonia. Notably, the film containing RPFE exhibited distinct color changes (from purple-red to pink) when shrimp spoiled. Our results suggest the structural, physical and functional properties of betacyanins-rich films are closely related to the source and type of betacyanins.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.04.152DOI Listing
April 2021

Corrigendum: Vitamin B12 Enhances Nerve Repair and Improves Functional Recovery After Traumatic Brain Injury by Inhibiting ER Stress-Induced Neuron Injury.

Front Pharmacol 2021 12;12:598335. Epub 2021 Apr 12.

Department of Emergency, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China.

[This corrects the article DOI: 10.3389/fphar.2019.00406.].
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http://dx.doi.org/10.3389/fphar.2021.598335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075574PMC
April 2021

Bidirectional interaction of nobiletin and gut microbiota in mice fed with a high-fat diet.

Food Funct 2021 Apr;12(8):3516-3526

Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, New Jersey 08901, USA.

Nobiletin is abundant in citrus peels and demonstrates good anti-obesity bioactivity. However, its anti-obesity mechanisms still remain unclear. This study aims to explore the bidirectional interaction between nobiletin and gut microbiota in mice fed with a high-fat diet. For the colonic bioconversion, more demethylated metabolites with higher biological activity were found in feces than nobiletin in the 48 h excretion study and 8 week consecutive dosing study. Moreover, long-term oral intake of nobiletin would modify the gut microbiota with improved demethylation ability and enhanced production of short chain fatty acids. The comparison of metabolite profiles in mouse liver and feces indicated that gut microbiota might have a higher biotransformation activity on nobiletin than the host. Two bacteria at the genus level, Allobaculum and Roseburia, remained enriched by nobiletin after the 4- and 8-week feedings. They might correlate with the enhanced nobiletin biotransformation and actively contribute to the health benefits of nobiletin in vivo. These results suggested that the bidirectional interaction of nobiletin and gut microbiota played an important role on the anti-obesity effect of nobiletin.
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http://dx.doi.org/10.1039/d1fo00126dDOI Listing
April 2021

Pleural biomarkers in diagnostics of malignant pleural effusion: a narrative review.

Transl Lung Cancer Res 2021 Mar;10(3):1557-1570

Department of Laboratory Medicine, the Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China.

Although cytology and pleural biopsy of pleural effusion (PE) are the gold standards for diagnosing malignant pleural effusion (MPE), these tools' diagnostic accuracy is plagued by some limitations such as low sensitivity, considerable inter-observer variation and invasiveness. The assessment of PE biomarkers may hence be seen as an objective and non-invasive diagnostic alternative in MPE diagnostics. In this review, we summarize the characteristics and diagnostic accuracy of available PE biomarkers, including carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), carbohydrate antigens 125 (CA125), carbohydrate antigen 19-9 (CA19-9), carbohydrate antigen 15-3 (CA15-3), a fragment of cytokeratin 19 (CYFRA 21-1), chitinase-like proteins (CLPs), vascular endothelial growth factor (VEGF) and its soluble receptor, endostatin, calprotectin, cancer ratio, homocysteine, apolipoprotein E (Apo-E), B7 family members, matrix metalloproteinase (MMPs) and tissue-specific inhibitors of metalloproteinases (TIMPs), reactive oxygen species modulator 1 (Romo1), tumor-associated macrophages (TAMs) and monocytes, epigenetic markers (e.g., cell-free microRNA and mRNA). We summarized the evidence from systematic review and meta-analysis for traditional tumor markers' diagnostic accuracy. According to the currently available evidence, we conclude that the traditional tumor markers have high specificity (around 0.90) but low sensitivity (around 0.50). The diagnostic accuracy of novel tumor markers needs to be validated by further studies. None of these tumor biomarkers would have sufficient diagnostic accuracy to confirm or exclude MPE when used alone. A multi-biomarker strategy, also encompassing the use of artificial intelligence algorithms, may be a valuable perspective for improving the diagnostic accuracy of MPE.
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http://dx.doi.org/10.21037/tlcr-20-1111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044497PMC
March 2021

The cortical organization of writing sequence: evidence from observing Chinese characters in motion.

Brain Struct Funct 2021 Jun 18;226(5):1627-1639. Epub 2021 Apr 18.

State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing, 100875, China.

Writing sequences play an important role in handwriting of Chinese characters. However, little is known regarding the integral brain patterns and network mechanisms of processing Chinese character writing sequences. The present study decoded brain patterns during observing Chinese characters in motion by using multi-voxel pattern analysis, meta-analytic decoding analysis, and extended unified structural equation model. We found that perception of Chinese character writing sequence recruited brain regions not only for general motor schema processing, i.e., the right inferior frontal gyrus, shifting, and inhibition functions, i.e., the right postcentral gyrus and bilateral pre-SMA/dACC, but also for sensorimotor functions specific for writing sequences. More importantly, these brain regions formed a cooperatively top-down brain network where information was transmitted from brain regions for general motor schema processing to those specific for writing sequences. These findings not only shed light on the neural mechanisms of Chinese character writing sequences, but also extend the hierarchical control model on motor schema processing.
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http://dx.doi.org/10.1007/s00429-021-02276-xDOI Listing
June 2021

Corrigendum to "Characterization and antioxidative activities of polysaccharide in Chinese angelica and its processed products" [Int. J. Biol. Macromol. 67 (2014) 195-200].

Int J Biol Macromol 2021 May 15;178:616. Epub 2021 Apr 15.

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.

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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.095DOI Listing
May 2021

Language switching training modulates the neural network of non-linguistic cognitive control.

PLoS One 2021 15;16(4):e0247100. Epub 2021 Apr 15.

State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, P. R. China.

Bilingual language experience, such as switching between languages, has been shown to shape both cognitive and neural mechanisms of non-linguistic cognitive control. However, the neural adaptations induced by language switching remain unclear. Using fMRI, the current study examined the impact of short-term language switching training on the neural network of domain-general cognitive control for unbalanced Chinese-English bilinguals. Effective connectivity maps were constructed by using the extended unified structural equation models (euSEM) within 10 common brain regions involved in both language control and domain-general cognitive control. Results showed that, the dorsal anterior cingulate cortex/pre-supplementary motor area (dACC/pre-SMA) lost connection from the right thalamus after training, suggesting that less neural connectivity was required to complete the same domain-general cognitive control task. These findings not only provide direct evidence for the modulation of language switching training on the neural interaction of domain-general cognitive control, but also have important implications for revealing the potential neurocognitive adaptation effects of specific bilingual language experiences.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247100PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049316PMC
April 2021

[Effect of DR4 Demethylation to the Proliferation and Apoptosis of Myeloid Leukemia K562 Cells].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Apr;29(2):422-427

Xinxiang Medical College, Xinxiang 453003, Henan Province, China.

Objective: To investigate the effect of tumor necrosis factor death receptor (DR) 4 demethylation to the proliferation and apoptosis of myeloid leukemia K562 cells.

Methods: The logarithmic phase of K562 cells were treated by desitabine (DCA) at 0, 0.8, 1.6 and 3.2 μmol/L, and the cells were divided into control group, DCA low dose group, DCA medium dose group and DCA high dose group respectively. The cells in control group were treated by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) 0.5 μg/ml for 24 h, and the cells were divided into TRAIL group. The cells in DCA high dose group were treated by TRAIL 0.5 μg/ml for 24 h, and were divided into DCA high dose + TRAIL group. Methylation-specific polymerase chain reaction (MS-PCR) was used to measure the methylation status of the DR4 gene promoter in the control group and DCA low, medium and high dose groups. Real-time fluorescent quantitative polymerase chain reaction (qRT-PCR) and Western blot were used to determine the relative expression of DR4 mRNA and protein in the control group and DCA low, medium and high dose groups. Dime- thylthiazole (MTT) method was used to determine the inhibition rate of cell proliferation of the cells in control group, DCA high dose group, TRAIL group, DCA high dose + TRAIL group. Flow cytometry was used to determine the apoptotic rate of the cells in control group, DCA high dose group, TRAIL group, DCA high dose + TRAIL group.

Results: The cells in the control group were methylation-positive, the brightness of the methylation bands of the cells in the DCA low, medium, and high dose groups was gradually decreased to disappear, and the DCA high dose group showed negative for methylation. The relative expression of DR4 mRNA and protein in the control group, DCA low, medium and high dose groups was increased sequentially (r=0.624, 0.704). The inhibition rate of cell proliferation of the cells in the control group, DCA high dose group, TRAIL group, DCA high dose + TRAIL group was increased sequentially (r=0.653, 0.754, 0.709, 0.725) at 24, 48 and 72 h.

Conclusion: DCA can reverse the methylation level of DR4 gene promoter in ML K562 cells and up-regulate the expression of DR4, which may enhance the proliferation inhibition and apoptosis promotion effects of TRAIL on K562 cells.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.02.018DOI Listing
April 2021

Real-time ultrasound helps determine the position of PICC tip in premature infants nursing.

Asian J Surg 2021 May 27;44(5):780. Epub 2021 Mar 27.

Department of Liver Surgery & Liver Transplantation, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, 610041, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2021.02.017DOI Listing
May 2021

Risk factors of late-onset postpartum pre-eclampsia in women without hypertension history: To whom should we give take-home messages?

Asian J Surg 2021 May 20;44(5):767. Epub 2021 Mar 20.

Department of Liver Surgery & Liver Transplantation, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, 610041, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2021.02.028DOI Listing
May 2021

Proteomic Analysis of -Induced Mechanism in Grafted Watermelon Seedlings.

Front Plant Sci 2021 4;12:632758. Epub 2021 Mar 4.

Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement/Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

Grafting can improve the resistance of watermelon to soil-borne diseases. However, the molecular mechanism of defense response is not completely understood. Herein, we used a proteomic approach to investigate the molecular basis involved in grafted watermelon leaf defense against f.sp. () infection. The bottle gourd rootstock-grafted (RG) watermelon seedlings were highly resistant to compared with self-grafted (SG) watermelon plants, with a disease incidence of 3.4 and 89%, respectively. Meanwhile, grafting significantly induced the activity of pathogenesis-related proteases under challenge. Proteins extracted from leaves of RG and SG under inoculation were analyzed using two-dimensional gel electrophoresis. Thirty-nine differentially accumulated proteins (DAPs) were identified and classified into 10 functional groups. Accordingly, protein biosynthetic and stress- and defense-related proteins play crucial roles in the enhancement of disease resistance of RG watermelon seedlings, compared with that of SG watermelon seedlings. Proteins involved in signal transduction positively regulated the defense process. Carbohydrate and energy metabolism and photosystem contributed to energy production in RG watermelon seedlings under infection. The disease resistance of RG watermelon seedlings may also be related to the improved scavenging capacity of reactive oxygen species (ROS). The expression profile of 10 randomly selected proteins was measured using quantitative real-time PCR, among which, 7 was consistent with the results of the proteomic analysis. The functional implications of these proteins in regulating grafted watermelon response against are discussed.
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http://dx.doi.org/10.3389/fpls.2021.632758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969889PMC
March 2021