Publications by authors named "Man Li"

1,090 Publications

  • Page 1 of 1

Precision therapy with quinidine of KCNT1-related epileptic disorders: a systematic review.

Br J Clin Pharmacol 2022 Aug 8. Epub 2022 Aug 8.

Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Aims: Despite numerous studies on quinidine therapies for epilepsies associated with KCNT1 gene mutations, there is no consensus on its clinical utility. Thus, we reviewed studies evaluating the efficacy and safety of quinidine in KCNT1-related epileptic disorders.

Methods: Electronic databases were queried for in vivo and in vitro studies on quinidine therapy in KCNT1-related epilepsies published on or before May 1st, 2022. The evaluation of evidence was done as per the American Academy of Neurology's classification scheme. Identification of significant factors that possibly influenced therapeutic effects of quinidine were performed using χ tests.

Results: Twenty-seven studies containing 82 patient records were reviewed. Records of eighty patients with 33 KCNT1 mutations were analyzed, of which 20 patients had gained ≥50% seizure reduction due to quinidine therapy. However, quinidine therapy often had different effects on patients with the same KCNT1 mutation. Age, genotypes of KCNT1 mutations, seizure types and brain MRI did not significantly influence the therapeutic effect of quinidine. Prolonged QTc was the most common among all adverse events with quinidine. Notably, results of in vitro quinidine tests did not correspond with in vivo tests.

Conclusions: Therapeutic effects of quinidine on KCNT1-related epilepsies remained indefinite as contradictory results were detected in similar patients. Age, seizure types, genotypes of KCNT1 mutations and brain MRI did not influence the therapeutic effects of quinidine. Insensitivity to quinidine by a certain Kcnt1 genotype in molecular tests predictive of its inefficacy in human populations of the respective mutation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/bcp.15479DOI Listing
August 2022

Long Non-Coding RNA and mRNA Profiles in the Spinal Cord of Rats with Resiniferatoxin-Induced Neuropathic Pain.

J Pain Res 2022 30;15:2149-2160. Epub 2022 Jul 30.

Department of Acupuncture, Wuhan First Hospital, Wuhan, Hubei Province, 430030, People's Republic of China.

Purpose: The ultrapotent transient receptor potential vanilloid 1 (TRPV1) agonist resiniferatoxin (RTX) induces small-fiber sensory neuropathy, which has been widely used model of postherpetic neuralgia to study mechanisms of neuropathic pain and new analgesics. The long non-coding RNA (lncRNA) and mRNA expression profiles in spinal dorsal horn tissues of rats six weeks after RTX injection to identify new RNAs related to neuropathic pain.

Methods: Microarray technology was applied to determine lncRNA expressions in spinal dorsal horn samples of adult rats 6 weeks after treatment with RTX or vehicle. The lncNA/mRNA co-expression network was constructed, and differential expression patterns of lncRNA and mRNA in RTX-treated rats were identified. Differential expressions of lncRNAs and mRNAs between RTX-treated samples and control samples were examined by RT-qPCR.

Results: Microarray analyses showed that 745 mRNA and 139 lncRNAs were upregulated, whereas 590 mRNA and 140 lncRNAs were downregulated in spinal dorsal horn tissues after RTX exposure. TargetScan was used to predict mRNA targets for these lncRNAs, which showed that the transcripts with multiple predicted target sites were related to neurologically important pathways. In addition, differential expressions of lncRNA (ENSRNOG00000022535, ENSRNOG00000042027, NR_027478, NR_030675) and Apobec3b mRNA in spinal cord tissue samples were validated, which confirmed the microarray data. The association between NR_030675 and Apobec3b levels was confirmed, which may be related to neuropathic pain.

Conclusion: Our study reveals lncRNA and mRNA of molecule targets that are enriched in the spinal cord dorsal horn and provides new information for further investigation on the mechanisms and therapeutics of neuropathic pain.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/JPR.S368599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348574PMC
July 2022

Prediction of Neurological Deterioration After Intracerebral Hemorrhage: The SIGNALS Score.

J Am Heart Assoc 2022 Aug 2;11(15):e026379. Epub 2022 Aug 2.

Department of Neurology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei Province China.

Background Intracerebral hemorrhage is the most disabling and lethal form of stroke. We aimed to develop a novel clinical score for neurological deterioration during hospitalization after intracerebral hemorrhage. Methods and Results We analyzed data from the CHERRY (Chinese Cerebral Hemorrhage: Mechanism and Intervention) study. Two-thirds of eligible patients were randomly allocated into the training cohort (n=1027) and one-third into the validation cohort (n=515). Multivariable logistic regression was used to identify factors associated with neurological deterioration (an increase in National Institutes of Health Stroke Scale of ≥4 or death) within 15 days after symptom onset. A prediction score was developed based on regression coefficients derived from the logistic model. The site, size, gender, National Institutes of Health Stroke Scale, age, leukocyte, sugar (SIGNALS) score was developed as a sum of individual points (0-8) based on site (1 point for infratentorial location), size (3 points for >20 mL of supratentorial hematoma volume or 2 points for >10 mL of infratentorial hematoma volume), sex (1 point for male sex), National Institutes of Health Stroke Scale score (1 point for >10), age (1 point for ≥70 years), white blood cell (1 point for>9.0×10/L), and fasting blood glucose (1 point>7.0 mmol/L). The proportion of patients who suffered from neurological deterioration increased with higher SIGNALS score, showing good discrimination and good calibration in the training cohort (C statistic, 0.821; Hosmer-Lemeshow test, =0.687) and in the validation cohort (C statistic, 0.848; Hosmer-Lemeshow test, =0.592), respectively. Conclusions The SIGNALS score reliably predicts the risk of in-hospital neurological deterioration of patients with intracerebral hemorrhage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1161/JAHA.122.026379DOI Listing
August 2022

Ultrasound-based radiomics technology in fetal lung texture analysis prediction of neonatal respiratory morbidity.

Sci Rep 2022 Jul 26;12(1):12747. Epub 2022 Jul 26.

Obstetrics and Gynecology Hospital of Fudan University, No.128, Shenyang Road, Shanghai, 200090, China.

To develop a novel method for predicting neonatal respiratory morbidity (NRM) by ultrasound-based radiomics technology. In this retrospective study, 430 high-throughput features per fetal-lung image were extracted from 295 fetal lung ultrasound images (four-chamber view) in 295 single pregnancies. Images had been obtained between 28 and 37 weeks of gestation within 72 h before delivery. A machine-learning model built by RUSBoost (Random under-sampling with AdaBoost) architecture was created using 20 radiomics features extracted from the images and 2 clinical features (gestational age and pregnancy complications) to predict the possibility of NRM. Of the 295 standard fetal lung ultrasound images included, 210 in the training set and 85 in the testing set. The overall performance of the neonatal respiratory morbidity prediction model achieved AUC of 0.88 (95% CI 0.83-0.92) in the training set and 0.83 (95% CI 0.79-0.97) in the testing set, sensitivity of 84.31% (95% CI 79.06-89.44%) in the training set and 77.78% (95% CI 68.30-87.43%) in the testing set, specificity of 81.13% (95% CI 78.16-84.07%) in the training set and 82.09% (95% CI 77.65-86.62%) in the testing set, and accuracy of 81.90% (95% CI 79.34-84.41%) in the training set and 81.18% (95% CI 77.33-85.12%) in the testing set. Ultrasound-based radiomics technology can be used to predict NRM. The results of this study may provide a novel method for non-invasive approaches for the prenatal prediction of NRM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-022-17129-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325724PMC
July 2022

Electroacupuncture Reduces Anxiety Associated With Inflammatory Bowel Disease By Acting on Cannabinoid CB1 Receptors in the Ventral Hippocampus in Mice.

Front Pharmacol 2022 8;13:919553. Epub 2022 Jul 8.

Department of Neurobiology, School of Basic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China.

The therapeutic effects of electroacupuncture (EA) on the comorbidity of visceral pain and anxiety in patients with inflammatory bowel disease (IBD) is well known. It has been known that the ventral hippocampus (vHPC) and the cannabinoid type 1 receptors (CB1R) are involved in regulating anxiety and pain. Therefore, in this study, we determined whether EA reduces visceral pain and IBD-induced anxiety CB1R in the vHPC. We found that EA alleviated visceral hyperalgesia and anxiety in TNBS-treated IBD mice. EA reversed over-expression of CB1R in IBD mice and decreased the percentage of CB1R-expressed GABAergic neurons in the vHPC. Ablating CB1R of GABAergic neurons in the vHPC alleviated anxiety in TNBS-treated mice and mimicked the anxiolytic effect of EA. While ablating CB1R in glutamatergic neurons in the vHPC induced severe anxiety in wild type mice and inhibited the anxiolytic effect of EA. However, ablating CB1R in either GABAergic or glutamatergic neurons in the vHPC did not alter visceral pain. In conclusion, we found CB1R in both GABAergic neurons and glutamatergic neurons are involved in the inhibitory effect of EA on anxiety but not visceral pain in IBD mice. EA may exert anxiolytic effect downregulating CB1R in GABAergic neurons and activating CB1R in glutamatergic neurons in the vHPC, thus reducing the release of glutamate and inhibiting the anxiety circuit related to vHPC. Thus, our study provides new information about the cellular and molecular mechanisms of the therapeutic effect of EA on anxiety induced by IBD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2022.919553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305710PMC
July 2022

Copper-thiosemicarbazone complexes conjugated-cellulose fibers: Biodegradable materials with antibacterial capacity.

Carbohydr Polym 2022 Oct 8;294:119839. Epub 2022 Jul 8.

National Local Joint Engineering Laboratory for Advanced Textile Processing and Clean Production, Wuhan Textile University, Wuhan 430073, Hubei, PR China; College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, Hubei, PR China; College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China. Electronic address:

Personal protective equipment (PPE) is vital in battling bacteria crisis, but conventional PPE materials lack antimicrobial activities and environmental friendliness. Our work focused on developing biodegradable and antibacterial fibers as promising bioprotective materials. Here, we grafted highly effective antibacterial copper-thiosemicarbazone complexes (CT1-4) on cellulose fibers via covalent linkages. Multiple methods were used to characterize the chemical composition or morphology of CT1-4 conjugated-fibers. Conjugation of CT1-4 maintains the mechanical properties (Breaking strength: 2.35-2.45 cN/dtex, Breaking elongation: 7.19 %-7.42 %) and thermal stability of fibers. CT1 can endow cellulose fibers with the excellent growth inhibition towards Escherichia coli (E. coli) (GIR: 61.5 % ± 1.28 %), Staphylococcus aureus (S. aureus) (GIR: 85.7 % ± 1.93 %), and Bacillus subtilis (B. subtilis) (GIR: 87.6 % ± 1.44 %). We believe that the application of CT1 conjugated-cellulose fibers is not limited to the high-performance PPE, and also can be extended to various types of protective equipment for food and medicine safety.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carbpol.2022.119839DOI Listing
October 2022

Prediction of Neurological Deterioration After Intracerebral Hemorrhage: The SIGNALS Score.

J Am Heart Assoc 2022 Jul 20:e026379. Epub 2022 Jul 20.

Department of Neurology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei Province China.

Background Intracerebral hemorrhage is the most disabling and lethal form of stroke. We aimed to develop a novel clinical score for neurological deterioration during hospitalization after intracerebral hemorrhage. Methods and Results We analyzed data from the CHERRY (Chinese Cerebral Hemorrhage: Mechanism and Intervention) study. Two-thirds of eligible patients were randomly allocated into the training cohort (n=1027) and one-third into the validation cohort (n=515). Multivariable logistic regression was used to identify factors associated with neurological deterioration (an increase in National Institutes of Health Stroke Scale of ≥4 or death) within 15 days after symptom onset. A prediction score was developed based on regression coefficients derived from the logistic model. The site, size, gender, National Institutes of Health Stroke Scale, age, leukocyte, sugar (SIGNALS) score was developed as a sum of individual points (0-8) based on site (1 point for infratentorial location), size (3 points for >20 mL of supratentorial hematoma volume or 2 points for >10 mL of infratentorial hematoma volume), sex (1 point for male sex), National Institutes of Health Stroke Scale score (1 point for >10), age (1 point for ≥70 years), white blood cell (1 point for>9.0×10/L), and fasting blood glucose (1 point>7.0 mmol/L). The proportion of patients who suffered from neurological deterioration increased with higher SIGNALS score, showing good discrimination and good calibration in the training cohort (C statistic, 0.821; Hosmer-Lemeshow test, =0.687) and in the validation cohort (C statistic, 0.848; Hosmer-Lemeshow test, =0.592), respectively. Conclusions The SIGNALS score reliably predicts the risk of in-hospital neurological deterioration of patients with intracerebral hemorrhage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1161/JAHA.121.026379DOI Listing
July 2022

LncRNA CTBP1-DT-encoded microprotein DDUP sustains DNA damage response signalling to trigger dual DNA repair mechanisms.

Nucleic Acids Res 2022 Aug;50(14):8060-8079

Program of Cancer Research, Affiliated Guangzhou Women and Children's Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, China.

Sustaining DNA damage response (DDR) signalling via retention of DDR factors at damaged sites is important for transmitting damage-sensing and repair signals. Herein, we found that DNA damage provoked the association of ribosomes with IRES region in lncRNA CTBP1-DT, which overcame the negative effect of upstream open reading frames (uORFs), and elicited the novel microprotein DNA damage-upregulated protein (DDUP) translation via a cap-independent translation mechanism. Activated ATR kinase-mediated phosphorylation of DDUP induced a drastic 'dense-to-loose' conformational change, which sustained the RAD18/RAD51C and RAD18/PCNA complex at damaged sites and initiated RAD51C-mediated homologous recombination and PCNA-mediated post-replication repair mechanisms. Importantly, treatment with ATR inhibitor abolished the effect of DDUP on chromatin retention of RAD51C and PCNA, thereby leading to hypersensitivity of cancer cells to DNA-damaging chemotherapeutics. Taken together, our results uncover a plausible mechanism underlying the DDR sustaining and might represent an attractive therapeutic strategy in improvement of DNA damage-based anticancer therapies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/nar/gkac611DOI Listing
August 2022

Magnetically Recyclable -SO-Coated Nanoparticles Promote Gas Storage via Forming Hydrates.

ACS Appl Mater Interfaces 2022 Jul 18. Epub 2022 Jul 18.

Key Laboratory of Ocean Energy Utilization and Energy Conservation of the Ministry of Education, Dalian University of Technology, Dalian 116024, China.

Efficient gas enrichment approaches are of great importance for the storage and transportation of clean energy and the sequestration of carbon dioxide. Of special interest is the regulated gas hydrate-based method; however, its operation requires adequate additives to overcome the low-storage capacity issue. Thus, this method is not economically feasible or environmentally friendly. In this work, a novel recyclable hydrate promoter of copolystyrene-sodium [email protected] (PNS) nanoparticles with an integrated core-shell structure was synthesized through emulsion polymerization. This was found to effectively reduce the induction time of methane hydrate formation by one-third compared with the widely used sodium dodecyl sulfate (SDS); the corresponding gas storage capacity was also comparable, up to 155 v/v. In addition, the PNS nanoparticles showed a good performance in foam inhibition upon hydrate decomposition, which frequently occurred with the use of SDS and other surfactant-based promoters. In particular, the new promoters contributed to a more than 30% increase in CO storage capacity, coacting with the fine sediments that mimic a marine environment. This provided further possibilities of sequestering CO in the form a gas hydrate under the seafloor. The underlying mechanism was proposed to involve anchored surfactants on the surface and tiny channels between the nanoparticles that lead to rapid hydrate nucleation and controlled growth. The results showed that the integrated magnetically recovering nanoparticles developed in this study could improve the efficiency of gas storage by forming gas hydrates; the excellent recycling performance paved the way for solving the economic and environmental problems encountered in additive usage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.2c06230DOI Listing
July 2022

Paeonol protects against doxorubicin-induced cardiotoxicity by promoting Mfn2-mediated mitochondrial fusion through activating the PKCε-Stat3 pathway.

J Adv Res 2022 Jul 14. Epub 2022 Jul 14.

National & Local Joint Engineering Research Center of Biodiagnosis and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China; Center for Tumor and Immunology, the Precision Medical Institute, the Second Affiliated Hospital of Xi' an Jiaotong University, Xi'an, Shaanxi 710004, China. Electronic address:

Introduction: The anti-cancer medication doxorubicin (Dox) is largely restricted in clinical usage due to its significant cardiotoxicity. The only medication approved by the FDA for Dox-induced cardiotoxicity is dexrazoxane, while it may reduce the sensitivity of cancer cells to chemotherapy and is restricted for use. There is an urgent need for the development of safe and effective medicines to alleviate Dox-induced cardiotoxicity.

Objectives: The objective of this study was to determine whether Paeonol (Pae) has the ability to protect against Dox-induced cardiotoxicity and if so, what are the underlying mechanisms involved.

Methods: Sprague-Dawley rats and primary cardiomyocytes were used to create Dox-induced cardiotoxicity models. Pae's effects on myocardial damage, mitochondrial function, mitochondrial dynamics and signaling pathways were studied using a range of experimental methods.

Results: Pae enhanced Mfn2-mediated mitochondrial fusion, restored mitochondrial function and cardiac performance both in vivo and in vitro under the Dox conditions. The protective properties of Pae were blunted when Mfn2 was knocked down or knocked out in Dox-induced cardiomyocytes and hearts respectively. Mechanistically, Pae promoted Mfn2-mediated mitochondria fusion by activating the transcription factor Stat3, which bound to the Mfn2 promoter in a direct manner and up-regulated its transcriptional expression. Furthermore, molecular docking, surface plasmon resonance and co-immunoprecipitation studies showed that Pae's direct target was PKCε, which interacted with Stat3 and enabled its phosphorylation and activation. Pae-induced Stat3 phosphorylation and Mfn2-mediated mitochondrial fusion were inhibited when PKCε was knocked down. Furthermore, Pae did not interfere with Dox's antitumor efficacy in several tumor cells.

Conclusion: Pae protects the heart against Dox-induced damage by stimulating mitochondrial fusion via the PKCε-Stat3-Mfn2 pathway, indicating that Pae might be a promising therapeutic therapy for Dox-induced cardiotoxicity while maintaining Dox's anticancer activity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jare.2022.07.002DOI Listing
July 2022

Efficacy and Safety of Long-Pulsed 755-nm Alexandrite Laser for Keratosis Pilaris: A Split-Body Randomized Clinical Trial.

Dermatol Ther (Heidelb) 2022 Aug 15;12(8):1897-1906. Epub 2022 Jul 15.

Department of Dermatology, Capital Medical University, Beijing Friendship Hospital, Beijing, 100051, China.

Introduction: Keratosis pilaris (KP) is a disfiguring disease and is resistant to treatment. Several treatment methods are available, but the efficacy is limited. This prospective, rater-blinded, split-body comparative study investigated the efficacy and safety of long-pulsed 755-nm alexandrite laser in the treatment of KP.

Methods: Twenty-two patients with KP of bilateral arms were enrolled in this study. All participants were randomized and treated with a long-pulsed 755-nm alexandrite laser on the left or right arm in four sessions held 3 weeks apart. The unified moisturizing lotion was applied on both left and right arms once a day. Physicians' assessment scores and patients' self-assessment scores were recorded, and skin imaging changes in dermoscopy, high-frequency ultrasound, and skin biopsy were obtained at baseline and 4 weeks after the fourth treatment.

Results: Of the 21 patients who completed the study, 15 were women and 6 were men. At 4 weeks after the fourth treatment, the laser side showed significantly lower total (2.0 versus 4.5), roughness (1.0 versus 2.0), and redness (1.0 versus 2.0) scores according to physicians' assessment (all P < 0.05). Furthermore, the laser side showed significantly lower total (2.0 versus 4.0), roughness (1.0 versus 2.0), and redness scores (1.0 versus 2.0) according to the patients' self-assessment (all P < 0.05). The proportions of patients who achieved dermoscopically and ultrasonographically showed excellent improvements in follicular plugs (57.1% versus 14.3%), perifollicular erythema (52.4% versus 9.5%), perifollicular hyperpigmentation (47.6% versus 14.3%), and the number of epidermal bulges (57.1% versus 19.1%) in the laser side was significantly higher than those who achieved such improvements in the control side (all P < 0.05). Histopathology showed that the follicular plugs and inflammatory cell infiltration were improved at the final visit. Three patients exhibited reversible postinflammatory hyperpigmentation.

Conclusion: Long-pulsed 755-nm alexandrite laser treatment is effective and safe in treating both skin roughness and redness in KP.

Trial Registration Number: ChiCTR2100054489.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13555-022-00771-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357583PMC
August 2022

Machine Learning Based on MRI DWI Radiomics Features for Prognostic Prediction in Nasopharyngeal Carcinoma.

Cancers (Basel) 2022 Jun 30;14(13). Epub 2022 Jun 30.

Department of Nuclear Medicine, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai 519099, China.

Purpose: This study aimed to explore the predictive efficacy of radiomics analyses based on readout-segmented echo-planar diffusion-weighted imaging (RESOLVE-DWI) for prognosis evaluation in nasopharyngeal carcinoma in order to provide further information for clinical decision making and intervention.

Methods: A total of 154 patients with untreated NPC confirmed by pathological examination were enrolled, and the pretreatment magnetic resonance image (MRI)-including diffusion-weighted imaging (DWI), apparent diffusion coefficient (ADC) maps, T2-weighted imaging (T2WI), and contrast-enhanced T1-weighted imaging (CE-T1WI)-was collected. The Random Forest (RF) algorithm selected radiomics features and established the machine-learning models. Five models, namely model 1 (DWI + ADC), model 2 (T2WI + CE-T1WI), model 3 (DWI + ADC + T2WI), model 4 (DWI + ADC + CE-T1WI), and model 5 (DWI + ADC + T2WI + CE-T1WI), were constructed. The average area under the curve (AUC) of the validation set was determined in order to compare the predictive efficacy for prognosis evaluation.

Results: After adjusting the parameters, the RF machine learning models based on extracted imaging features from different sequence combinations were obtained. The invalidation sets of model 1 (DWI + ADC) yielded the highest average AUC of 0.80 (95% CI: 0.79-0.81). The average AUCs of the model 2, 3, 4, and 5 invalidation sets were 0.72 (95% CI: 0.71-0.74), 0.66 (95% CI: 0.64-0.68), 0.74 (95% CI: 0.73-0.75), and 0.75 (95% CI: 0.74-0.76), respectively.

Conclusion: A radiomics model derived from the MRI DWI of patients with nasopharyngeal carcinoma was generated in order to evaluate the risk of recurrence and metastasis. The model based on MRI DWI can provide an alternative approach for survival estimation, and can reveal more information for clinical decision-making and intervention.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cancers14133201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264891PMC
June 2022

The role of toll-like receptors (TLRs) in pan-cancer.

Ann Med 2022 12;54(1):1918-1937

Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Toll-like receptors (TLRs) are important components of the innate and adaptive immune systems, and abnormal TLR expression has been linked to a variety of cancers. However, there was a lack of clarity on the association of TLR stimulation with the carcinogenesis of cancer. The study's goal was to analyse the clinical importance of TLRs expression at the mRNA level in pan-cancer datasets, as well as the link between TLR expression and carcinogenesis, progression, and clinical prognosis.

Methods: The expression profile of TLRs derived from UCSC pan-cancer data was analysed in multiple dimensions, including clinical analysis, immunological subtype analysis, tumour microenvironment (TME) analysis, tumour stem cell correlation analysis, and drug sensitivity analysis. Additionally, we analyse protein-protein interactions, functional enrichment, and chromatin accessibility, as well as TLR expression in single-cell sequencing data.

Results: Our multi-omics analysis results imply that TLRs may operate as a biological marker for carcinogenesis and progression, a potential target for anti-tumour therapy, and a prognostic biomarker, laying the theoretical groundwork for future translational medicine research.

Conclusion: TLRs are involved in the formation of malignancies and can be explored in further detail as potential prognostic indicators. Key MessagesToll-like receptors (TLRs) are key factors in the process of the innate and adaptive immune response, and their aberrant expression of TLRs have been widely reported in various cancer. However, the association between TLRs stimulation and tumorigenesis of cancer has not been well clarified.In this study, in the pan-cancer data, integrated TLR family gene expression analysis, clinical correlation analysis, immune subtype correlation analysis, tumour microenvironment correlation analysis, tumour stem cell correlation analysis, and drug sensitivity correlation analysis were performed.TLRs play an important role in the development of tumours and can be studied in depth as potential prognostic markers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/07853890.2022.2095664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9272932PMC
December 2022

Benzo[b]naphtho[1,2-d]thiophene Sulfoxides: Biomimetic Synthesis, Photophysical Properties, and Applications.

Angew Chem Int Ed Engl 2022 Jul 6:e202203908. Epub 2022 Jul 6.

School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei Province, China.

A practical synthesis of nonsymmetrical thiophene-fused aromatic systems has been developed that was inspired by the biodegradation of benzothiophene. For the first time, the photophysical properties of a series of π-conjugated benzo[b]naphtho[1,2-d]thiophene (BNT) sulfoxides were explored both in solution and in the solid state. The excellent fluorescence characteristics enable various applications of these compounds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202203908DOI Listing
July 2022

Contributes to the Maintenance of the Cancer Stem-like Phenotype in Non-Small Cell Lung Cancer by Regulating Histone Deacetylase 8.

Ann Clin Lab Sci 2022 May;52(3):439-451

Anhui Province Key Laboratory of Clinical and Preclinical Research in Respiratory Disease; Molecular Diagnosis Center, Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.

Objective: Cancer stem-like cells (CSLCs) are closely associated with tumor recurrence, metastasis, and drug-resistance. is related to tumorigenesis and development of non-small cell lung cancer (NSCLC). The role and regulatory mechanism of in CSLCs of NSCLC remain unclear. This study aimed to identify the biological characteristics of CSLCs and the role of in maintaining stemness of NSCLC.

Methods: H1299-spheres and A549-spheres were obtained by oncosphere-forming assay and CSLCs by flow cytometry. Expression of CD133, aldehyde dehydrogenase isoform 1, E-cadherin, vimentin, and histone deacetylase 8 (HDAC8) was tested using immunofluorescence staining, qRT-PCR, and Western blotting. CCK-8 and Transwell assays were employed to determine proliferation, migration, and invasion ability of CSLCs and adherent monolayer cells in NSCLC. We regulated expression and HDAC activity in CSLCs to explore the mechanism of in stemness maintenance and analyzed expression of target proteins in NSCLC tissues.

Results: Compared with monolayer cells, CSLCs showed a decreased response to cisplatin-mediated inhibition of proliferation, increased migration and invasion, and high expression of and HDAC8, accompanied by EMT marker alterations. Targeted knockdown of in CSLCs of NSCLC resulted in diminished stemness phenotypes and HDAC8 expression, whereas inhibition of HDAC activity affected stemness maintenance. Moreover, the expression of target proteins showed consistent changes in NSCLC tissues.

Conclusions: Compared with monolayer cells, cancer stem-like phenotype properties of NSCLC were altered, was involved in stemness maintenance of CSLCs, and this process may be related to the activation of HDAC8.
View Article and Find Full Text PDF

Download full-text PDF

Source
May 2022

EGCG-coated silver nanoparticles self-assemble with selenium nanowires for treatment of drug-resistant bacterial infections by generating ROS and disrupting biofilms.

Nanotechnology 2022 Jul 19;33(41). Epub 2022 Jul 19.

School of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, People's Republic of China.

Bacterial infections pose a serious threat to human health, and the development of new antibiotics has not kept pace with the development of bacterial resistance. Therefore, there is an urgent need to design antibiotic-like nano-formulations that break through bacterial resistance mechanisms. In this work, we successfully synthesized a safe and effective antibacterial nano-formulation of [email protected]@EGCG by self-assembly of epigallocatechin gallate (EGCG)-coated silver nanoparticles (Ag) on the surface of selenium nanowires (Se). Thebacteriostatic results showed that 40g [email protected]@EGCG had significant antibacterial activity against drug-resistant() and() by destroying the formation of bacterial biofilm, promoting the production of high concentration reactive oxygen species and destroying bacterial cell wall. In addition, the results ofantibacterial experiments showed that subcutaneous administration of 10 mg kgof [email protected]@EGCG could promote wound healing by reducing apoptosis and inflammatory responses in infected wounds. It is worth mentioning that the reduced and modified [email protected]@EGCG by this natural product has negligibletoxicity. This development strategy of nano-antibacterial materials, which breaks through the drug resistance mechanism, provides new ideas for the development of drugs for drug-resistant bacterial infections.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ac7db0DOI Listing
July 2022

Development of Designer Transcription Activator-Like Effector-Based Plant Growth Regulator for Higher Yield in Rice.

Front Plant Sci 2022 14;13:924645. Epub 2022 Jun 14.

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

Recent studies have shown that reprogramming of gene expression in a genome can induce the production of proteins enabling yield increase. The transcription activator-like effectors (TALEs) from several species of bacterial have been extensively studied, and a series of research tools, such as genome editing tool TALENs and gene expression activators, have been developed based on the specific protein-nucleic acid recognition and binding mechanisms of TALEs. In this proof-of-principle study, we designed and constructed a designer TALE (dTALE), designated as dTALE-NOG1, to specifically target the promoter of gene in rice, and demonstrated that this dTALE can be used as a new type of plant growth regulator for better crop growth and harvest. In doing so, the dTALE-NOG1 was transferred into the non-pathogenic pv. () strain PH to generate a genetically engineered bacteria (GEB) strain called PH-dtNOG1. Functional verification showed that dTALE-NOG1 could significantly induce the expression of . By spraying cell suspension of PH-dtNOG1 on the rice plants during the tillering stage, the transcription level of was highly enhanced, the grain number of rice plants was increased by more than 11.40%, and the grain yield per plant increased by more than 11.08%, demonstrating that the dTALE-NOG1 was highly effective in enhancing rice yield. This work provided a new strategy for manipulating agronomical traits by reprogramming gene expression in a crop genome.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2022.924645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237611PMC
June 2022

Ectopic Expression of Executor Gene Enhances Resistance to Both Bacterial and Fungal Diseases in Rice.

Int J Mol Sci 2022 Jun 11;23(12). Epub 2022 Jun 11.

National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agriculture Sciences (CAAS), Beijing 100081, China.

Bacterial blight (BB) and bacterial leaf streak (BLS), caused by phytopathogenic bacteria pv. () and pv. (), respectively, are the most serious bacterial diseases of rice, while blast, caused by (. ), is the most devastating fungal disease in rice. Generating broad-spectrum resistance to these diseases is one of the key approaches for the sustainable production of rice. Executor () genes are a unique type of plant resistance () genes, which can specifically trap transcription activator-like effectors (TALEs) of pathogens and trigger an intense defense reaction characterized by a hypersensitive response in the host. This strong resistance is a result of programed cell death induced by the gene expression that is only activated upon the binding of a TALE to the effector-binding element (EBE) located in the gene promoter during the pathogen infection. Our previous studies revealed that the gene has the broadest and highest resistance to BB. To investigate whether the -mediated resistance is efficient against pv. (), the causal agent of BLS, we generated a new version of , designated as , to specifically trap the conserved TALEs from multiple strains. The results showed that the confers broad resistance against both BB and BLS in rice. Moreover, our further experiment on the transgenic plants firstly demonstrated that the -gene-mediated defensive reaction is also effective against . , the causal agent of the most devastating fungal disease in rice. Our current work provides a new strategy to exploit the full potential of the -gene-mediated disease resistance in rice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms23126545DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224217PMC
June 2022

Integrated Analysis of Glutathione Metabolic Pathway in Pancreatic Cancer.

Front Cell Dev Biol 2022 3;10:896136. Epub 2022 Jun 3.

Program of Cancer Research, Affiliated Guangzhou Women and Children's Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China.

Metabolic enzyme-genes (MEs) play critical roles in various types of cancers. However, MEs have not been systematically and thoroughly studied in pancreatic cancer (PC). Global analysis of MEs in PC will help us to understand PC progressing and provide new insights into PC therapy. In this study, we systematically analyzed RNA sequencing data from The Cancer Genome Atlas (TCGA) (n = 180 + 4) and GSE15471 (n = 36 + 36) and discovered that metabolic pathways are disordered in PC. Co-expression network modules of MEs were constructed using weighted gene co-expression network analysis (WGCNA), which identified two key modules. Both modules revealed that the glutathione signaling pathway is disordered in PC and correlated with PC stages. Notably, glutathione peroxidase 2 (), an important gene involved in glutathione signaling pathway, is a hub gene of the key modules. Analysis of immune microenvironment components reveals that PC stage is associated with M2 macrophages, the marker gene of which is significantly correlated with . The results indicated that is associated with PC progression, providing new insights for future targeted therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2022.896136DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204196PMC
June 2022

Genetic loci and prioritization of genes for kidney function decline derived from a meta-analysis of 62 longitudinal genome-wide association studies.

Kidney Int 2022 Jun 16. Epub 2022 Jun 16.

The George Institute for Global Health, University of New South Wales, Sydney, Australia.

Estimated glomerular filtration rate (eGFR) reflects kidney function. Progressive eGFR-decline can lead to kidney failure, necessitating dialysis or transplantation. Hundreds of loci from genome-wide association studies (GWAS) for eGFR help explain population cross section variability. Since the contribution of these or other loci to eGFR-decline remains largely unknown, we derived GWAS for annual eGFR-decline and meta-analyzed 62 longitudinal studies with eGFR assessed twice over time in all 343,339 individuals and in high-risk groups. We also explored different covariate adjustment. Twelve genome-wide significant independent variants for eGFR-decline unadjusted or adjusted for eGFR-baseline (11 novel, one known for this phenotype), including nine variants robustly associated across models were identified. All loci for eGFR-decline were known for cross-sectional eGFR and thus distinguished a subgroup of eGFR loci. Seven of the nine variants showed variant-by-age interaction on eGFR cross section (further about 350,000 individuals), which linked genetic associations for eGFR-decline with age-dependency of genetic cross-section associations. Clinically important were two to four-fold greater genetic effects on eGFR-decline in high-risk subgroups. Five variants associated also with chronic kidney disease progression mapped to genes with functional in-silico evidence (UMOD, SPATA7, GALNTL5, TPPP). An unfavorable versus favorable nine-variant genetic profile showed increased risk odds ratios of 1.35 for kidney failure (95% confidence intervals 1.03-1.77) and 1.27 for acute kidney injury (95% confidence intervals 1.08-1.50) in over 2000 cases each, with matched controls). Thus, we provide a large data resource, genetic loci, and prioritized genes for kidney function decline, which help inform drug development pipelines revealing important insights into the age-dependency of kidney function genetics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.kint.2022.05.021DOI Listing
June 2022

TMEM158 Regulates the Canonical and Non-Canonical Pathways of TGF-β to Mediate EMT in Triple-Negative Breast Cancer.

J Cancer 2022 21;13(8):2694-2704. Epub 2022 May 21.

Department of Oncology & Department of Breast Surgery, The Second Hospital of Dalian Medical University, Dalian, 116023 China.

Triple-negative breast cancer (TNBC) is a highly aggressive form of breast cancer with high metastatic potential. To date, no directed treatment options have been developed for the treatment of metastatic or advanced TNBC. The oncogene TMEM158, also known as RIS1, is upregulated by Ras-induced cellular senescence. Although TMEM158 has been shown to be involved in tumor progression, little is known about the molecular function and expression of TMEM158 in breast cancer. The present study evaluated the expression and prognostic relevance of TMEM158 in breast cancer patients from several databases. Gene set enrichment analysis (GSEA) showed that TMEM158 was closely associated with epithelial-mesenchymal transition (EMT) and TGF-β pathways. Gain- and loss-of-function assays indicated that overexpressed TMEM158 might participate in EMT by activating the TGF-β pathway, which in turn promotes tumor migration, invasion, and metastasis. These findings suggest that TMEM158 has the potential to become a new therapeutic target for TNBC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.65822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174849PMC
May 2022

Differential and shared genetic effects on kidney function between diabetic and non-diabetic individuals.

Commun Biol 2022 Jun 13;5(1):580. Epub 2022 Jun 13.

Division of Kidney, Urologic and Hematologic Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.

Reduced glomerular filtration rate (GFR) can progress to kidney failure. Risk factors include genetics and diabetes mellitus (DM), but little is known about their interaction. We conducted genome-wide association meta-analyses for estimated GFR based on serum creatinine (eGFR), separately for individuals with or without DM (n = 178,691, n = 1,296,113). Our genome-wide searches identified (i) seven eGFR loci with significant DM/noDM-difference, (ii) four additional novel loci with suggestive difference and (iii) 28 further novel loci (including CUBN) by allowing for potential difference. GWAS on eGFR among DM individuals identified 2 known and 27 potentially responsible loci for diabetic kidney disease. Gene prioritization highlighted 18 genes that may inform reno-protective drug development. We highlight the existence of DM-only and noDM-only effects, which can inform about the target group, if respective genes are advanced as drug targets. Largely shared effects suggest that most drug interventions to alter eGFR should be effective in DM and noDM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s42003-022-03448-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192715PMC
June 2022

Triglyceride-Glucose Index and the Prognosis of Patients with Acute Ischemic Stroke: A Meta-Analysis.

Horm Metab Res 2022 Jun 13;54(6):361-370. Epub 2022 Jun 13.

Department of Neurology, Fourth Hospital of Harbin Medical University, Harbin, China.

A higher triglyceride-glucose (TyG) index has been related to an increased incidence of stroke in community population. A meta-analysis was performed to evaluate the association between TyG index and prognosis in patients with acute ischemic stroke (IS). Observational studies, which evaluated the influence of TyG index on functional outcome and mortality in patients with acute IS were retrieved by search the PubMed, Embase, Web of Science, Wanfang and China National Knowledge Infrastructure databases from inception to February 20, 2022. Two authors independently collected the data of study characteristics and outcomes. A random-effect model was used to pool the results via incorporating the influence of possible between-study heterogeneity. Eight cohort studies involving 34 076 patients with acute IS contributed to the study. Pooled results showed that a higher TyG index was independently associated with increased risks of all-cause mortality (OR: 1.60, 95% CI: 1.19-2.15, p=0.002; I2=78%) and poor functional outcome (OR: 1.37, 95% CI: 1.11-1.69, p=0.004; I2=71%). Further sensitivity analyses by excluding one cohort study at a time showed consistent results (p all<0.05). Subgroup analyses showed similar results in prospective and retrospective cohort studies, in non-diabetic and diabetic patients, and in studies with follow-up durations within 3 months and of 12 months (p for subgroup analyses all>0.05). In conclusion, higher TyG index is a predictor of all-cause mortality and poor functional outcome in patients with acute IS, and TyG index may be useful for prognostic evaluation in these patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1055/a-1853-9889DOI Listing
June 2022

Association of the Weight-Adjusted-Waist Index With Risk of All-Cause Mortality: A 10-Year Follow-Up Study.

Front Nutr 2022 25;9:894686. Epub 2022 May 25.

Department of Geriatrics, the 2nd Medical Center, Chinese PLA General Hospital, Beijing, China.

Background: To explore the relationship between weight-adjusted-waist index (WWI) and the risk of all-cause mortality in one urban community-dwelling population in China.

Methods: This is a prospective cohort study with a sample of 1,863 older adults aged 60 years or over in Beijing who completed baseline examinations in 2009-2010 and a 10-year follow-up in 2020. WWI was calculated as waist circumference (cm) divided by the square root of weight (kg). Cox regression analysis was performed to investigate the significance of the association of WWI with all-cause mortality. The area under the receiver operating characteristic (ROC) curves were used to compare the ability of each obesity index to predict mortality.

Results: During a median follow-up of 10.8 years (1.0 to 11.3 years), 339 deaths occurred. After adjusted for covariates, the hazard ratios (HRs) for all-cause mortality progressively increased across the tertile of WWI. Compared with the lowest WWI category (tertile1 <10.68 cm/√kg), with WWI 10.68 to 11.24cm/√kg, and≥11.25 cm/√kg, the HRs (95% confidence intervals (CIs)) for all-cause mortality were 1.58 (1.12-2.22), and 2.66 (1.80-3.92), respectively. In stratified analyses, the relationship between WWI and the risk of all-cause mortality persisted. The area under ROC for WWI was higher for all-cause mortality than BMI, WHtR, and WC.

Conclusion: WWI was associated with a higher risk for all-cause mortality, and the association was more robust with the highest WWI category.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnut.2022.894686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174751PMC
May 2022

Evaluation of China Healthy Lifestyle for All Interventions Based on RE-AIM Framework - China, 2007-2020.

China CDC Wkly 2022 May;4(19):403-406

Department of Epidemiology and Health Statistics, School of Public Health, Hebei Medical University, Hebei Key Laboratory of Environment and Population Health, Shijiazhuang City, Hebei Province, China.

What Is Already Known About This Topic?: Since the launch of China Healthy Lifestyle for All (CHLA), each action area has been evaluated at the local level and effective results have been achieved in most areas.

What Is Added By This Report?: Based on the Reach, Effectiveness, Adoption, Implementation, and Maintenance (RE-AIM) model, the study found that there is an imbalance in the development of CHLA, and some of the action goals and indicators are not satisfactory.

What Are The Implications For Public Health Practices?: A national action evaluation index system should be built to ensure the sustainability and scientific nature of this strategy. In addition, the government needs to attach great importance to CHLA to effectively help residents take health actions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.46234/ccdcw2022.091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167605PMC
May 2022

Quantitative Analysis on NCDs Prevention and Control Policies Based on the Perspective of Policy Instruments - China, 1990-2020.

China CDC Wkly 2022 May;4(19):397-402

NCDs Control and Prevention Society, Chinese Preventive Medicine Association, Beijing, China.

What Is Already Known About This Topic?: Noncommunicable diseases (NCDs) are a major public health problem in the world. NCDs are the leading cause of premature deaths and disabilities among Chinese residents, resulting in heavy economic and health burdens.

What Is Added By This Report?: This study conducted a quantitative analysis of the policy texts on NCDs prevention and control in China from 1990 to 2020, based on the perspective of policy instruments. It was discovered that China's NCDs prevention and control policies developed rapidly from the ground up over the 30 years from 1990 to 2020 and that the majority of China's NCDs prevention and control policies were environment-oriented, while supply-oriented and demand-oriented policies were insufficient.

What Are The Implications For Public Health Practice?: The findings of this study suggested that increasing supply-oriented and demand-oriented strategies should be prioritized in the future formulation and revision of NCDs prevention and control policies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.46234/ccdcw2022.090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167603PMC
May 2022

Recommendations and Future Direction for Population-Based Prevention and Control of Behavioral Risk Factors for NCDs.

China CDC Wkly 2022 May;4(19):407-410

NCDs Control and  Prevention  Society,  Chinese  Preventive  Medicine  Association, Beijing,  China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.46234/ccdcw2022.092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167604PMC
May 2022

Formation mechanisms of CsPbBr/CsPbBr microscale composites assisted with imidazolium cations and their device applications.

Dalton Trans 2022 Jun 21;51(24):9445-9453. Epub 2022 Jun 21.

State Key Laboratory of Luminescent Materials and Devices, and Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, South China University of Technology, Guangzhou 510641, China.

The instability of all-inorganic perovskite nanocrystals (NCs) remains a major challenge to their practical applications in displays and lighting. In order to improve their environmental resistance, highly luminescent CsPbBr NC embedded CsPbBr microcrystals (MCs) have been fabricated by an anti-solvent reprecipitation method with the assistance of 1-alkyl-3-methyl-imidazolium bromides. The heterostructure of the MCs is investigated in detail, and their formation mechanism is discussed in terms of the dissolution-precipitation equilibria and the total energy of variously scaled nanoparticles density functional theory (DFT) calculations. Although the imidazolium ligands are not directly capping with the inner CsPbBr NCs, they are important to the formation of these MCs. The MCs exhibit better thermal resistance compared to conventional CsPbBr NCs prepared by the hot-injection method. Additionally, a prototype white light-emitting diode (WLED) was fabricated to demonstrate its practical application prospects. Thanks to the narrow emission bands and enhanced stability of the MCs, the WLED shows greater performance compared to bare perovskite NCs, indicating its great potential as a green phosphor in lighting and display applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2dt00842dDOI Listing
June 2022

Rapamycin Attenuates Anxiety and Depressive Behavior Induced by Helicobacter pylori in Association with Reduced Circulating Levels of Ghrelin.

Neural Plast 2022 30;2022:2847672. Epub 2022 May 30.

Department of Gastroenterology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.

Background: Helicobacter pylori (H. pylori) infection is closely associated with depression and development of neuroinflammation. The aim of this study is to explore the relationship between H. pylori, depression, and circulating levels of ghrelin.

Methods: Mice were randomly divided into three groups: healthy control group (gavaged sterile saline and injected with saline, = 8); H. pylori+saline group (gavaged H. pylori and injected with saline, = 8); and H. pylori+rapa group (gavaged H. pylori and injected with rapamycin, = 8). Open field test (OFT), sucrose preference test (SPT), forced swim test (FST), and tail suspension test (TST) were used for anxiety and depressive behavior test. Western blotting was utilized to assess mTOR, p-mTOR, and GSMD expression, and serum ghrelin levels were estimated using ELISA.

Results: In the OFT, the control mice moved more and exhibited a increase in crossing number relative to the H. pylori+saline mice (all < 0.05). Increased quantity of fecal boli can be indicative of increased anxiety and emotionality of the subject animal. H. pylori+saline mice exhibited an increase in fecal boli when compared to control mice and H. pylori+rapa mice ( < 0.05). H. pylori infected mice decreasing the expression of ghrelin. The protein levels of p-mTOR/mTOR in the gastric antrum mTOR signaling activation and low-level ghrelin in H. pylori-infect mice compared to those in control mice (all  <0.001). Compared with single H. pylori infection, mTOR inhibitors increased the ghrelin secretion of H. pylori infection to a certain extent ( < 0.05). The protein levels of GSDMD expression significantly increase in hippocampus of H. pylori-infected mice ( < 0.001). Rapamycin treatment inhibited expression of GSDMD in H. pylori-infected mice ( < 0.05).

Conclusions: H. pylori infection is associated with increased expression of mTOR and decreased circulating levels of ghrelin. Elevated pyroptosis in the brain and anxiety- and depressed-like behaviors occur when ghrelin levels are suppressed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/2847672DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170403PMC
May 2022
-->