Publications by authors named "Mahsa Hadipour Jahromy"

10 Publications

  • Page 1 of 1

Pyrrolopyridine and Isoindole as potential anticonvulsant agents: Design, synthesis and pharmacological evaluation.

Curr Comput Aided Drug Des 2022 May 11. Epub 2022 May 11.

Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Tehran Islamic Azad Medical Sciences University. Tehran, Iran.

Background And Objective: Phthalimide as the rigid form of ameltolide exhibits a phenytoin-like profile of drug-receptor interaction and is active in the MES model and inactive in the PTZ model as anti-epileptic agent. In this research, based on the isosteric replacement, we have reported the design, preparation, and antiepileptic activity of 13 new analogs of pyrrolopyridine and isoindole.

Methods: The designed compounds were prepared by condensing 3, 4-pyridine dicarboxylic anhydride or 4-fluorophthalic anhydride with different respective aryl amines. MES and PTZ induced seizure models were done to evaluate the antiepileptic effect of the prepared ligands.

Results: The prepared ligands have significantly affect both tonic and clonic seizures. In tonic seizures, the prepared compounds decrease mortality to a significant extent and in clonic seizures showed better frequency and latency significantly. Compounds 9, 12 and 13 were the most potent ligands that were more potent than phenytoin.

Conclusion: It is concluded that the best distance between two aryl parts is two bonds and the substitution of the nitro group at the meta position of the phenyl ring is better than para position. Our research group has been investigating this concept for designing newer compounds with better anticonvulsant activity.
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http://dx.doi.org/10.2174/1573409918666220512000247DOI Listing
May 2022

Antidiabetic effects of the ethanolic extract of R.M. Fritsch on streptozotocin-induced diabetes in a mice model.

Food Sci Nutr 2021 Sep 18;9(9):4815-4826. Epub 2021 Jun 18.

Department of Basic Sciences and Pathobiology Faculty of Veterinary Medicine Razi University Kermanshah Iran.

Medicinal plants can protect different organs against diabetes-induced oxidative stress due to their antioxidant compounds. The present study was designed to investigate the potential of R.M. Fritsch (A. saralicum) ethanolic extract to alleviate the adverse effects of streptozotocin (STZ)-induced diabetes in male BALB/c mice. Seventy male mice were randomly divided into seven groups ( = 10). Diabetes was experimentally induced by STZ (60 mg/kg bw). . ethanolic extract with doses 5, 20, 80, and 320 mg/kg was administrated for 20 consecutive days in diabetic animals. Based on the obtained results, the untreated diabetic mice showed high blood glucose level, cholesterol, low-density lipoprotein (LDL), white blood cells count (WBC), and platelets, as well as liver enzymes, urea, and creatinine. Administration of different doses of . extract significantly reduced blood glucose level similar to glibenclamide. Also, the levels of catalase and superoxide dismutase enzymes restored toward normal level. All hepatic and renal function parameters as well as hematological parameters were improved following treatment with . extract particularly at high doses. Histopathological studies showed a decrease in hepatic, renal, and pancreatic damage after treatment with . extract. The results of the present work indicate that . ethanolic extract can attenuate diabetic hepato-renal, pancreatic, and hematological damages.
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http://dx.doi.org/10.1002/fsn3.2405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441324PMC
September 2021

Protective Effects of on Diazinon-Induced Brain Damage in Young Male Mice.

Prev Nutr Food Sci 2020 Mar;25(1):65-70

Herbal Pharmacology Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran 19168, Iran.

Diazinon is an imminent and hazardous chemical organophosphate multiplex that is generally used as a pesticide but is toxic for many species particularly vertebrates. Berberry ( L., family Berberidaceae) is a plant that flourishes in Europe and Asia that has been largely investigated for its therapeutic effects. In the present study, we evaluated the protective effects of on diazinon-induced brain damage in young male mice. Twenty-one young male albino mice weighing 18±2 g were divided in three equal groups of seven mice, and treated orally with either olive oil (control), diazinon 50 mg/kg+ extract 200 mg/kg, or diazinon 50 mg/kg. After three weeks, cerebrum and cerebellum samples were collected for antioxidant assays. The results indicated that diazinon increased oxidative stress in the brain of mice. The glutathione content and proceedings of antioxidant enzymes, such as glutathione peroxidase, superoxide dismutase, and catalase, were significantly reduced in both the cerebellum and cerebrum of diazinon-treated mice, compared with the control group. In addition, acetylcholinesterase (AChE) activity was inhibited by exposure to this pesticide. Administration of 200 mg/kg extract with diazinon significantly decreased oxidative stress indices in all experiments. The results indicated that extract has protective effects against lipid peroxidation of the cerebellum and cerebrum, and in regenerating AChE activity in the brain induced by diazinon.
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http://dx.doi.org/10.3746/pnf.2020.25.1.65DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143012PMC
March 2020

An improvement in acute wound healing in rats by the synergistic effect of photobiomodulation and arginine.

Lab Anim Res 2019 11;35:28. Epub 2019 Dec 11.

8Price Institute of Surgical Research, University of Louisville and Noveratech LLC of Louisville, Louisville, KY USA.

In this probe, at first we examined the best route and dosage of arginine administration on wound healing in an excisional wound model in rats. Next, we intend to assess the impact of photobiomodulation (PBM) and arginine, individually and together, on the wound healing. In the pilot study, an excisional wound was made in each of 24 rats. There were 4 groups. Group 1 was the control group. In groups 2 and 3, wounds were topically treated with arginine ointments (ARG.) 2% and 5%, respectively. In group 4, arginine was injected (ARG. INJ.,i.p.). In the main phase, in 24 new rats, an excisional wound was made. There were 4 groups: group 5 served as the control. Wounds in group 6 were topically treated with ARG 2%. Wounds in group 7 were subjected to PBM. Wounds in group 8 were treated with PBM+ARG. 2%. On day 15, wound area measurement, wound strength, and stereological examination were performed. In the pilot study, we found that the ARG 2% ointment significantly decreased wound area than ARG. 5%, ARG. INJ. and control groups, and significantly increased wound strength compared to the control and ARG.5% groups. In the main phase, a significant decrease of wound area in all treatment regimens was induced. PBM + ARG. 2% and PBM treatment regimens significantly improved wound strength and almost all stereological parameters, compared to the control and ARG. 2% groups. PBM + ARG. 2% induced anti-inflammatory and angiogenic activities, and hastened the wound healing process in an excisional wound model in rats.
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http://dx.doi.org/10.1186/s42826-019-0025-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081532PMC
December 2019

Effectiveness of Povidone-Iodine 1% Eye Drop on and Induced-Keratitis in Mice.

Galen Med J 2019 2;8:e1161. Epub 2019 Jun 2.

Herbal Pharmacology Research Center, Department of Pharmacology, Faculty of Medicine, Islamic Azad University, Tehran Medical Sciences, Tehran, Iran.

Background: Bacterial keratitis is an ophthalmic infection that may result in irreversible corneal damage. This study aimed to examine the effectiveness of povidone-iodine eye drop 1% in eye infection caused by inoculation of and of mice.

Materials And Methods: In this study, 49 adult male CBA/J mice were used that divided into seven equal groups. The corneas of all mice were scratched and infected with a clinical strain of either or topically, except control group. Subgroups received chloramphenicol 0.5% eye drop twice daily in case of infection or ciprofloxacin 0.3% eye drop every 4 hours following infection from or povidone-iodine 1% eye drop in both groups, from post infection (PI) day 3 to7. Slit lamp examinations (SLE) of the corneas and eyes were performed every day to examine detectable or intense corneal opacity and erosion.

Results: In all infected mice, SLE scores were significantly higher than the control group on PI day 3. Scores increased steadily by time in all infected groups without treatment, reached to maximal value on PI day 7. In infected groups, treatment with either povidone-iodine 1% or chloramphenicol 0.5% or ciprofloxacin 0.3% on day 3, significantly decreased the SLE scores on PI day 7.

Conclusion: Povidone-Iodine 1% was effective to decrease and induced-keratitis symptoms in mice. Treatment with povidone-iodine 1% was observed time-dependently and was comparable to common eye drop antibiotics.
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http://dx.doi.org/10.22086/gmj.v8i0.1161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343812PMC
June 2019

Anti-dyskinetic efficacy of 5-HT3 receptor antagonist in the hemi-parkinsonian rat model.

IBRO Rep 2019 Jun 14;6:40-44. Epub 2018 Dec 14.

Department of Pharmacology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad university, Tehran, Iran.

Parkinson's disease is a progressive debilitative neurodegenerative disease characterised mostly with bradykinesia, tremor, catatonia, drooping posture, unsteady gate and unstable steps. Levodopa has been proven to be among the most effective and acceptable treatment that can reconstitute dopamine in Parkinson's disease. However, there is a relation between levodopa long term administration and dyskinesia. Regarding the effectiveness of ondansetron in Parkinson's disease, we planned to test its effect on levodopa-induced dyskinesia (LID). In this study, Parkinsonism was induced in 40 adult male rats using 6-OHDA injection into the striatum via stereotaxic surgery. After 2 weeks, all animals tested for Parkinson's disease using apomorphine rotation test. Then, animals with positive symptoms for Parkinsonism divided into 4 equal groups, the first group treated with levodopa 50 mg/kg i.p, the second group received only distilled water, the third and forth groups treated with levodopa 50 mg/kg i.p plus two different doses of ondansetron (0.04 and 0.08 mg/kg i.p) for 3 weeks. Animals tested for dyskinesia using AIMs and rotarod tests at specific days and a week after discontinuation of ondansetron. Evaluations of AIMs test showed significant changes in dyskinetic movements and reduction in scores in groups treating with ondansetron when compared with the first group. Upon discontinuations of ondansetron in the last two groups, AIMs scores significantly increased. While in rotarod test, ondansetron had no additional benefit when added to levodopa in motor coordination of animals. Findings of this study suggest that co administration of ondansetron with levodopa is effective in attenuating dyskinesia.
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http://dx.doi.org/10.1016/j.ibror.2018.12.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302250PMC
June 2019

Effects of blackberry () fruit juice on levodopa-induced dyskinesia in a mice model of Parkinson's disease.

J Exp Pharmacol 2018 4;10:29-35. Epub 2018 Jul 4.

Herbal Pharmacology Research Center, Department of Pharmacology, Faculty of Medicine, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran,

Background And Objective: Levodopa-induced dyskinesia (LID) is a movement disorder that occurs due to levodopa consumption for a long period to attenuate Parkinsonism. Plants have been the basis for medical treatments in human history and still widely practiced. Blackberry () is one of the fruits rich in anthocyanin. The present study examined the effect of blackberry fruit juice on LID in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease in mice.

Materials And Methods: In this study, 42 male mice were used, which were divided into six groups equally: one control group and five groups receiving MPTP injection. After confirmation of Parkinsonism in MPTP groups, one group was preserved without treatment and four other groups were treated with levodopa (50 mg/kg ip). After the onset of LID (2 weeks), one group was kept without additional treatment and three other groups were treated with three different doses of blackberry fruit juice (5, 10, and 15 mL/kg) with levodopa orally for 7 days. Abnormal involuntary movement scale (AIMS) and cylinder behavioral test were carried out according to the schedule. The collected data were analyzed using the SPSS software with the significant level of <0.05.

Results: Parkinson's disease was confirmed with AIMS test on the fourth day after MPTP injection. The onset of LID was observed after 2 weeks of levodopa treatment using both behavioral tests. The result of administration of fruit juice for 1 week showed that this addition is useful in hindering LID. These effects were more pronounced at doses 10 and 15 mL/kg with nearly the same results on attenuating AIMS. Low dose of the fruit juice does not seem to affect LID significantly.

Conclusion: fruit juice is effective to attenuate LID in an MPTP-induced Parkinson mice model.
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http://dx.doi.org/10.2147/JEP.S161782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037159PMC
July 2018

The effect of chronic administration of methylphenidate on morphometric parameters of testes and fertility in male mice.

J Reprod Infertil 2012 Oct;13(4):232-6

Department of Anatomy, Tehran Medical Branch, Islamic Azad University, Tehran, Iran.

Background: Due to common use of methylphenidate (MPH) for the treatment of Attention Deficit Hyperactivity Disorder (ADHD) and the role of the reproductive system in the production of gametes, studying the effects of this medication on the morphometry of testes, serum testosterone concentration, leydig cells function, and fertility rate was the aim of this study.

Methods: Twenty seven male mice (Balb/C), eight weeks old, were randomly divided into one control and two treated groups. After weighing the mice, the treated groups received MPH (produced in Novartis company) at the doses of 2 mg/kg and 10 mg/kg for 40 days. The control group received only normal saline. Subsequently, after weighing the animals, the weights of testes, dimensions of the testis, and the serum testosterone concentration were measured in six mice belonging to each group. After tissue processing, the samples were stained with hematoxylin and eosin, then the leydig cells were counted. In order to assess male fertility in each group, 3 male mice were chosen and each of them was kept with three female mice in a separate cage. After 10 days, the fertility rates of the male mice were determined by counting the number of embryos in uterus and the corpora lutea in their ovaries.

Results: The results of this study revealed that prescription of different doses of MPH can cause a significant decrease of the body weight. It reduces the number of leydig cells, too (p<0.01). Moreover, serum testosterone concentration (67.72±8.24 ng/ml in control group and 0.302±0.416 ng/ml after treatment with 2 mg/kg/day MPH) and fertility rate (95.42%±4.68% in control group and 64.96%±18.51% after treatment with 2 mg/kg/day MPH) of the male mice declined significantly in the treated groups compared with the control group (p<0.01), but it did not cause any changes in the weight or morphometric parameters of testes.

Conclusion: The results of this study confirmed that MPH can negatively affect serum testosterone concentration and fertility rate of the male mice by decreasing the number of leydig cells and reducing the body weight.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3719348PMC
October 2012

Chondroprotective effects of pomegranate juice on monoiodoacetate-induced osteoarthritis of the knee joint of mice.

Phytother Res 2010 Feb;24(2):182-5

Department of Pharmacology, Tehran Medical Branch, Islamic Azad University, Tehran, Iran.

To study the effectiveness of pomegranate juice on osteoarthritis, mono-iodoacetate induced loss of articular cartilage in the mouse tibiofemoral joint was used as a model. Mono-iodoacetate is an inhibitor of glycolysis which promotes osteoarthritis similar to that noted in human osteoarthritis. The histopathology of the subchondral bone and cartilage of mouse knee joints treated with a single intra-articular injection of mono-iodoacetate (0.1 mg) and killed at 1, 14 and 28 days post injection was investigated. The effect of pomegranate juice (4 mL/kg, 10 mL/kg, 20 mL/kg, orally) was studied in different groups. Histopathological changes in knee joints were seen after 2 weeks. Early osteoarthritis was characterized by areas of chondrocyte degeneration, which sometimes involved the entire thickness of the articular cartilage in the tibial plateaus and femoral condyles. Changes to the subchondral bone and proteoglycan contents, focal fragmentation and collapse of bony trabeculae with fibrosis and necrosis, and synovial cell proliferation were observed. The administration of pomegranate juice dose dependently prevented the negative effects of iodoacetate. Chondrocyte damage was significantly prevented, with proteoglycan less affected, especially in the groups receiving a high amount of pomegranate juice. No cell proliferation or inflammatory cells were detected in the synovial fluid. The effectiveness of pomegranate juice in improving histopathological damage is emphasized and its chondroprotective effect in vivo highlighted.
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http://dx.doi.org/10.1002/ptr.2880DOI Listing
February 2010

Binary combinations of propofol and barbiturates on human alpha(1) glycine receptors expressed in Xenopus oocytes.

Eur J Pharmacol 2003 Sep;477(2):81-6

Welsh School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Avenue, CF10 3XF Cardiff, UK.

To test whether there is a common site of action for intravenous anaesthetics at the glycine receptor, the effects of binary combinations of thiopentone, pentobarbitone, methohexitone, and propofol have been tested on human alpha(1) glycine receptors expressed in Xenopus laevis oocytes using two-electrode voltage-clamp techniques. Thiopentone (5-40 microM), pentobarbitone (25-400 microM) and propofol (2-100 microM) (but not methohexitone), potentiated the glycine-induced (50 microM) current in a dose-dependent manner, with the maximum potentiation observed to be 218%, 400%, and 576%, respectively. In binary combination with thiopentone, pentobarbitone or propofol, methohexitone reduced potentiation compared to that by the individual anesthetics to 190%, 260% and 460%, respectively. Combination of thiopentone and pentobarbitone (50 microM) increased potentiation, compared to that by thiopentone alone. Binary combinations of propofol with either thiopentone or pentobarbitone showed more potentiation, compared to that observed with the individual anesthetics. Our results indicate that thiopentone, pentobarbitone and propofol all act as positive allosteric modulators at the alpha(1) glycine receptor. In contrast, methohexitone has no action alone but acts as a competitive antagonist to thiopentone, pentobarbitone and propofol. We suggest that, on the basis of these results, these four intravenous anaesthetics share a common site of action at the glycine receptor.
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http://dx.doi.org/10.1016/j.ejphar.2003.08.017DOI Listing
September 2003
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