Publications by authors named "Mahboubeh Adeli-Sardou"

14 Publications

  • Page 1 of 1

Rapid and Facile Microwave-Assisted Synthesis of Palladium Nanoparticles and Evaluation of Their Antioxidant Properties and Cytotoxic Effects Against Fibroblast-Like (HSkMC) and Human Lung Carcinoma (A549) Cell Lines.

Biol Trace Elem Res 2020 Sep 28;197(1):132-140. Epub 2019 Nov 28.

Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran.

We report here a simple microwave irradiation method (850 W, 3 min) for the synthesis of palladium nanoparticles (Pd NPs) using ascorbic acid (as reducing agent) and sodium alginate (as stabilizer agent). The synthesized nanoparticles were characterized using transmission electron microscopy (TEM), energy dispersive X-ray (EDX), X-ray diffraction spectroscopy (XRD), UV-Visible spectroscopy, and Fourier transform infrared spectroscopy (FTIR) techniques. Antioxidant properties and cytotoxic effects of as-synthesized Pd NPs and Pd (II) acetate were also assessed. UV-Vis study showed the formation of Pd NPs with maximum absorption at 345 nm. From TEM analysis, it was observed that the Pd NPs had spherical shape with particle size distribution of 13-33 nm. Based on DPPH radical scavenging activity and reducing power assay, the antioxidant activities of Pd NPs were significantly higher than the Pd (II) acetate (p < 0.05). At the same concentration of 640 μg/mL, the scavenging activities were 32.9 ± 3.2% (Pd (II) acetate) and 27.2 ± 2.1% (Pd NPs). For A549 cells treated 48 h with Pd NPs, Pd (II) acetate, and cisplatin, the measured concentration necessary causing 50% cell death (IC) was 7.2 ± 1.7 μg/mL, 32.1 ± 2.1 μg/mL, and 206.2 ± 3.5 μg/mL, respectively. On HSkMC cells, the IC of the Pd NPs (320 μg/mL) was higher compared to Pd (II) acetate (228.7 ± 3.6 μg/mL), which confirmed lower cytotoxicity of the Pd NPs.
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http://dx.doi.org/10.1007/s12011-019-01984-0DOI Listing
September 2020

Antimicrobial, anti-biofilm, and anti-proliferative activities of lipopeptide biosurfactant produced by Acinetobacter junii B6.

Microb Pathog 2020 Jan 17;138:103806. Epub 2019 Oct 17.

Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.

Lipopeptide biosurfactants (LPBs) are amphiphilic compounds produced by microorganisms exhibiting various biological activities. The main aim of the present study was to assess the in vitro antimicrobial, anti-biofilm, and cytotoxic effects of LPB produced by Acinetobacter junii (AjL). We determined AjL minimum inhibitory concentration (MIC) against both Gram-positive and Gram-negative bacteria as well as two fungal strains. Also, the anti-biofilm activity of AjL against the biofilm produced by clinically isolated bacterial strains was investigated. The AjL non-selectively showed activity against both Gram-positive and Gram-negative bacterial strains. The obtained results of the present study exhibited that the AjL in concentrations nearly below critical micelle concentration (CMC) has an effective antibacterial activity. It was found that the MIC values of AjL were lower than standard antifungal and it exhibited nearly 100% inhibition against Candida utilis. The attained results of the biofilm formation revealed that AjL disrupted the biofilm of Proteus mirabilis, Staphylococcus aureus, and Pseudomonas aeruginosa at 1250 μg/ml and 2500 μg/ml concentrations. The attained results of cytotoxic effect (determined by WST-1 assay) of the AjL revealed IC of 7.8 ± 0.4 mg/ml, 2.4 ± 0.5 mg/ml, and 5.7 ± 0.1 mg/ml, against U87, KB, and HUVEC cell lines, respectively. The results indicated that AjL has a potential application in the relatively new field of biomedicine.
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http://dx.doi.org/10.1016/j.micpath.2019.103806DOI Listing
January 2020

Antimicrobial and anti-biofilm activities of Bi subnitrate and BiNPs produced by sp. SFG against clinical isolates of , , and .

IET Nanobiotechnol 2019 Jun;13(4):377-381

Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.

In the present study sp. Shakibaie, Forootanfar, and Ghazanfari (SFG), was applied for preparation of biogenic Bi nanoparticles (BiNPs) and antibacterial and anti-biofilm activities of the purified BiNPs were investigated by microdilution and disc diffusion methods. Transmission electron micrographs showed that the produced nanostructures were spherical with a size range of 40-120 nm. The measured minimum inhibitory concentration of both the Bi subnitrate and BiNPs against three biofilms producing bacterial pathogens of , , and were found to be above 1280 µg/ml. Addition of BiNPs (1000 µg/disc) to antibiotic discs containing tobramycin, nalidixic acid, ceftriaxone, bacitracin, cefalexin, amoxicillin, and cefixime significantly increased the antibacterial effects against methicillin-resistant (MRSA) in comparison with Bi subnitrate ( < 0.05). Furthermore, the biogenic BiNPs decreased the biofilm formation of , , and to 55, 85, and 15%, respectively. In comparison to Bi subnitrate, BiNPs indicated significant anti-biofilm activity against ( < 0.05) while the anti-biofilm activity of BiNPs against and was similar to that of Bi subnitrate. To sum up, the attained results showed that combination of biogenic BiNPs with commonly used antibiotics relatively enhanced their antibacterial effects against MRSA.
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http://dx.doi.org/10.1049/iet-nbt.2018.5102DOI Listing
June 2019

Evaluation of Carum-loaded Niosomes on Breast Cancer Cells:Physicochemical Properties, In Vitro Cytotoxicity, Flow Cytometric, DNA Fragmentation and Cell Migration Assay.

Sci Rep 2019 05 9;9(1):7139. Epub 2019 May 9.

Department of Biotechnology, Institute of Science, High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.

Thymoquinone (TQ), a phytochemical compound found in Carum carvil seeds (C. carvil), has a lot of applications in medical especially cancer therapy. However, TQ has a hydrophobic nature, and because of that, its solubility, permeability and its bioavailability in biological mediums are poor. To diminish these drawbacks, we have designed a herbal carrier composed of Ergosterol (herbal lipid), Carum carvil extract (Carum) and nonionic surfactants for herbal cancer treatment. C. carvil was extracted and characterized by GC/Mass. Two different formulations containing TQ and Carum were encapsulated into niosomes (Nio/TQ and Nio/Carum, respectively) and their properties were compared together. Morphology, size, zeta potential, encapsulation efficiency (EE%), profile release rate, in vitro cytotoxicity, flow cytometric, DNA fragmentation and cell migration assay of formulations were evaluated. Results show that both loaded formulations have a spherical morphology, nanometric size and negative zeta potential. EE% of TQ and Carum loaded niosomes was about 92.32% ± 2.32 and 86.25% ± 1.85, respectively. Both loaded formulations provided a controlled release compared with free TQ. MTT assay showed that loaded niosomes have more anti-cancer activity compared with Free TQ and free Carum against MCF-7 cancer cell line and these results were confirmed by flow cytometric analysis. Cell cycle analysis showed G2/M arrest in TQ, Nio/TQ and Nio/Carum formulations. TQ, Nio/TQ and Nio/Carum decreased the migration of MCF7 cells remarkedly. These results show that the TQ and Carum loaded niosomes are novel carriers with high efficiency for encapsulation of low soluble phytochemicals and also would be favourable systems for breast cancer treatment.
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http://dx.doi.org/10.1038/s41598-019-43755-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509162PMC
May 2019

cytotoxicity studies of parent and nanoencapsulated Holmium-2,9-dimethyl-1,10-phenanthroline complex toward fish-salmon DNA-binding properties and antibacterial activity.

J Biomol Struct Dyn 2019 10 16;37(17):4437-4449. Epub 2019 Jan 16.

Department of Biotechnology, Institute of Science, High Technology & Environmental Science, Graduate University of Advance Technology , Kerman , Iran.

In this study, the interaction of Holmium (Ho) complex including 2, 9-dimethyl-1,10-phenanthroline, also called Neocuproine (Neo), [Ho(Neo)Cl.HO], as fluorescence probe with fish-salmon DNA (FS-DNA) is studied during experimental investigations. Multi-spectroscopic methods are utilized to determine the affinity binding constants () of complex-FS-DNA. It is found that fluorescence of Ho complex is strongly quenched by the FS-DNA through a static quenching procedure. Under optimal conditions in Tris(trishydroxymethyl-aminomethane)-HCl buffer at 25 °C with pH ≈ 7.2, intrinsic binding constant of Ho complex is 6.12 ± 0.04 × 10 M. Also, the binding site number and Stern-Volmer quenching constant are calculated. There are different approaches, including iodide quenching assay, salt effect and thermodynamical assessment to determine the features of the binding mode between Ho complex and FS-DNA. Also, the parent and starch and lipid nanoencapsulated Ho complex, as potent antitumor candidates, were synthesized. The main structure of Ho complex is maintained after encapsulation using starch and lipid nanoparticles. 3-[4,5-Dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method was used to assess the anticancer properties of Ho complex and its encapsulated forms on human cancer cell lines of human lung carcinoma cell line and breast cancer cell line. In conclusion, these compounds could be considered as new antitumor candidates. Communicated by Ramaswamy H. Sarma.
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http://dx.doi.org/10.1080/07391102.2018.1557077DOI Listing
October 2019

Cytotoxicity of biologically synthesised bismuth nanoparticles against HT-29 cell line.

IET Nanobiotechnol 2018 08;12(5):653-657

Sudent Research Committee, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.

This study was purposed to examine the cytotoxicity and functions of biologically synthesised bismuth nanoparticles (Bi NPs) produced by sp. SFG on human colon adenocarcinoma cell line of HT-29. The structural properties of Bi NPs were investigated using transmission electron microscopy, energy dispersive X-ray, and X-ray diffraction techniques. The cytotoxic effects of Bi NPs were analysed using flow cytometry cell apoptosis while western blot analyses were applied to analyse the cleaved caspase-3 expression. Oxidative stress (OS) damage was determined using the measurement of the glutathione (GSH) and malondialdehyde (MDA) levels and antioxidant activity of superoxide dismutase (SOD) and catalase (CAT) levels. The half maximal inhibitory concentration (IC) value of Bi NPs was measured to be 28.7 ± 1.4 µg/ml on HT-29 cell line. The viability of HT-29 represented a concentration-dependent pattern (5-80 µg/ml). The mode of Bi NPs induced apoptosis was found to be mainly related to late apoptosis or necrosis at IC concentration, without the effect on caspase-3 activities. Furthermore, Bi NPs reduced the GSH and increased the MDA levels and decreased the SOD and CAT activities. Taken together, biogenic Bi NPs induced cytotoxicity on HT-29 cell line through the activation of late apoptosis independent of caspase pathway and may enhance the OS biomarkers.
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http://dx.doi.org/10.1049/iet-nbt.2017.0295DOI Listing
August 2018

Antimicrobial and Antioxidant Activity of the Biologically Synthesized Tellurium Nanorods; A Preliminary Study.

Iran J Biotechnol 2017 29;15(4):268-276. Epub 2017 Dec 29.

Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.

Recent theranostic (therapeutic or diagnostic) applications of tellurium nanoparticles have attracted a great interest for development of different methods for synthesis of this valuable nanostructure, especially via biological resources. In the present study, the antimicrobial and antioxidant effects of the tellurium nanorods (Te NRs) biosynthesized by a bacterial strain strain Te were evaluated. The antimicrobial effect of Te NRs and potassium tellurite against different bacterial and fungal pathogens was assessed by microdilution method. Furthermore, the disk diffusion method was used to evaluate the antibacterial effect of the biogenic Te NRs and potassium tellurite against , alone or in combination with various antibiotics. Also, the biogenic Te NRs were investigated for antioxidant activity using 2, 2-diphenyl- 1-picrylhydrazyl (DPPH) scavenging activity and reducing power assay. Transmission electron micrograph (TEM) of the purified Te NRs showed individual and rod-shaped nanostructure (~22 nm diameter by 185 nm in length). Based on the data obtained from both microdilution and disk diffusion method the K TeO exhibited a higher antibacterial and antifungal activity compared to the Te NRs. The measured IC for the biogenic Te NRs (i.e. DPPH radical scavenging activity) was found to be 24.9 μg.mL, while, K TeO has represented only 17.6 ± 0.8 % DPPH radical scavenging effect at the concentration of 160 μg.mL. The reducing power assay revealed a higher electron-donating activity for Te NRs compared to KTeO. Based on the data obtained from both microdilution and disk diffusion method the KTeO exhibited a higher antimicrobial and antifungal activity than Te NRs. Te NRs didn't show the antibacterial effect against the tested bacterial strain: MRSA and showed an inhibitory effect and antibacterial activity of the effective antibiotics. However, more studies should be performed to explore the action mechanism of the produced biogenic Te NRs.
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http://dx.doi.org/10.15171/ijb.1580DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903914PMC
December 2017

Statistical optimization of kojic acid production by a UV-induced mutant strain of Aspergillus terreus.

Braz J Microbiol 2018 Oct - Dec;49(4):865-871. Epub 2018 Apr 24.

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran; Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. Electronic address:

The ability of four Aspergillus strains for biosynthesis of kojic acid was evaluated among which Aspergillus terreus represented the highest level (2.21g/L) of kojic acid production. Improvement kojic acid production ability of A. terreus by random mutagenesis using different exposure time to ultraviolet light (5-40min) was then performed to obtain a suitable mutant of kojic acid production (designated as C, 7.63g/L). Thereafter, design of experiment protocol was employed to find medium components (glucose, yeast extract, KHPO (NH)SO, and pH) influences on kojic acid production by the C mutant. A 2 fractional factorial design augmented to central composite design showed that glucose, yeast extract, and KHPO were the most considerable factors within the tested levels (p<0.05). The optimum medium composition for the kojic acid production by the C mutant was found to be glucose, 98.4g/L; yeast extract, 1.0g/L; and KHPO, 10.3mM which was theoretically able to produce 120.2g/L of kojic acid based on the obtained response surface model for medium optimization. Using these medium compositions an experimental maximum Kojic acid production (109.0±10g/L) was acquired which verified the efficiency of the applied method.
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http://dx.doi.org/10.1016/j.bjm.2018.03.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175716PMC
December 2018

Probiotic and antioxidant properties of selenium-enriched Lactobacillus brevis LSe isolated from an Iranian traditional dairy product.

J Trace Elem Med Biol 2017 Mar 23;40:1-9. Epub 2016 Nov 23.

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran. Electronic address:

The present study was designed to isolate a highly selenium-tolerant lactobacillus strain from an Iranian traditional dairy product named as Spar. Different criteria such as tolerance to the low pH, simulated gastric juice (SGJ), simulated intestinal juice (SIJ) and bile salts tolerance as well as Caco-2 cell adhesion assay were examined to evaluate the probiotic potentials of the selected isolate. Furthermore, the antioxidant properties of the isolate cultivated in medium containing and free of SeO ions were evaluated using DPPH scavenging and reducing power assays. The isolate was identified using conventional identification and 16S rDNA gene sequencing methods as Lactobacillus brevis LSe. The obtained results showed that the isolate was able to tolerate high concentration of sodium selenite (3.16mM). By decreasing the pH of the SGJ from 6 to 3, the survival percent of L. brevis LSe was not significantly changed over the time (p>0.05). In addition, the survival percent of the isolate in the SIJ (pH 6 and pH 8) was not statistically altered after 3h, 6h and 24h of incubation (p>0.05). In the presence of bile salts (0.3% and 0.6%) the survival rate of L. brevis LSe was not significantly decreased (p>0.05).L. brevis LSe also demonstrated the satisfactory ability to adhere to Caco-2 cells which were similar to that of the reference strain L. plantarum. The obtained results of antioxidant evaluation showed that L. brevis LSe containing elemental Se exhibited significantly higher radical scavenging ability (36.5±1.31%) and reducing power (OD, 0.14) than L. brevis LSe cultured in selenite-free medium (p<0.05). To sum up, further investigations should be conducted to merit the probable potential health benefit of Se-enriched L. brevis LSe and its application as Se-containing supplements or fermented foods.
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http://dx.doi.org/10.1016/j.jtemb.2016.11.013DOI Listing
March 2017

Microwave-assisted biosynthesis of zinc nanoparticles and their cytotoxic and antioxidant activity.

J Trace Elem Med Biol 2017 Jan 3;39:116-123. Epub 2016 Sep 3.

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran; Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran. Electronic address:

The present study was designed for microwave assisted synthesis of zinc nanoparticles (Zn NPs) using Lavandula vera leaf extract in the presence of ZnSO (1mM). The biogenic Zn NPs were then characterized using scanning electron microscopy (SEM), energy dispersive X-ray (EDX), X-ray diffraction spectroscopy (XRD), UV-visible spectroscopy, and Fourier transform infrared spectroscopy (FTIR) techniques. Thereafter, the cytotoxic effect of ZnSO and Zn NPs on different cell lines was investigated by MTT-based cytotoxicity assay and their antioxidant properties were assessed using DPPH scavenging activity and reducing power assay. The SEM micrograph showed that the Zn NPs had spherical shape with the size range of 30-80nm. For A549, MCF-7, HT-29, and Caco-2 cell lines treated with Zn NPs, the concentration necessary causing 50% cell death (IC) was found to be 22.3±1.1μgmL, 86±3.7μgmL, 10.9±0.5μgmL, and 56.2±2.8μgmL, respectively. In the case of ZnSO, the same results (IC) were observed at concentration of 81.6±1.3μgmL (A549), 121.0±2.4μgmL (MCF-7), 43.0±1.4μgmL (HT-29), and 85.7±2.3μgmL (Caco-2). The obtained results of antioxidant activity showed that the IC values of butylated hydroxyanisole (BHA) and Zn NPs were 44μgmLand 65.3μgmL, respectively, while ZnSO at concentration of 200μgmL exhibited only 10.9% DPPH radical scavenging effect. Moreover, the reducing power of Zn NPs and BHA were significantly higher than ZnSO (p<0.05). To sum up, application of L. vera leaf extract combined with microwave heating energy led to simple and fast formation of Zn nanostructures exhibited higher antioxidant and cytotoxic activity compared to soluble Zn ions. However, identification of the related mechanisms merit further studies.
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http://dx.doi.org/10.1016/j.jtemb.2016.09.001DOI Listing
January 2017

The Zn(II) nanocomplex: Sonochemical synthesis, characterization, DNA- and BSA-binding, cell imaging, and cytotoxicity against the human carcinoma cell lines.

J Fluoresc 2016 May 18;26(3):1007-20. Epub 2016 Mar 18.

Department of Biotechnology, Institute of Science, High Technology & Environmental Science, Graduate University of Advanced Technology, Kerman, Iran.

The focus of this article is preparation of a new kind of nanomaterial, the Zn(II) nanocomplex, to decrease growth of human carcinoma cell lines. The Zn(II) nanocomplex coordinated by phendione, [Zn(phendione)3](PF6)2 (where phendione is 1,10-phenanthroline-5,6-dione), has been synthesized by sonochemical method and characterized by FT-IR, dynamic light scattering (DLS), and scanning electron microscopy (SEM). The interaction of the complex and nanocomplex with fish sperm DNA (FS-DNA) has been investigated under physiological conditions by a series of experimental methods (fluorescence titration, viscosity, cyclic voltammetry (CV), competitive DNA-binding studies with ethidium bromide, and SEM). Results have indicated that the complex binds to FS-DNA by two biding modes, viz., electrostatic and partial insertion phendione between the base stacks of double-stranded DNA. The quenching constants (Ksv), binding constants (Kbin), and number of binding sites (n) at different temperatures, as well as thermodynamic parameters (ΔH(o), ΔS(o) and ΔG(o)) have been calculated for the BSA-complex system. Protein binding studies show that the complex and nanocomplex could bind with BSA. Results of synchronous fluorescence of BSA show that addition of the complex affect the microenvironment of both tyrosine and tryptophan residues during the binding process. The in vitro cytotoxicity of the complex and nanocomplex against the human carcinoma cell lines (MCF-7 and A-549) was evaluated by MTT assay. Results indicate that the complex and nanocomplex have greater cytotoxicity activity against MCF-7 with IC50 values of 0.2 and 0.9 mg/L, respectively. Results of the microscopic analyses of the cancer cells confirm results of cytotoxicity.
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http://dx.doi.org/10.1007/s10895-016-1788-1DOI Listing
May 2016

Sonochemical Synthesis and Characterization of the Copper(II) Nanocomplex: DNA- and BSA-Binding, Cell Imaging, and Cytotoxicity Against the Human Carcinoma Cell Lines.

J Fluoresc 2016 Mar 21;26(2):545-58. Epub 2015 Dec 21.

Department of Biotechnology, Institute of Science, High Technology & Environmental Science, Graduate University of Advanced Technology, Kerman, Iran.

The focus of the present work is the preparation of new metal-based nanodrug to overcome limitations of chemotherapy such as poor water solubility of most common chemotherapeutic drugs. The copper(II) complex of 1,2,4-triazine derivatives, [Cu(dppt)2(H2O)2](2+) (dppt is 5,6-diphenyl- 3- (2-pyridyl)-1,2,4-triazine), has been synthesized at nano-size by sonochemical method and characterized by FTIR, zetasizer, and scanning electron microscopy (SEM). The interaction of the complex and nanocomplex with fish sperm DNA (FS-DNA) and BSA have been investigated under physiological conditions by a series of experimental methods. The results have indicated that the complex binds to FS-DNA by two biding modes, viz., electrostatic and intercalates into the base pairs of DNA. The competitive study with ethidium bromide (EB) shows that the complex and nanocomplex competes for the DNA-binding sites with EB. Protein binding studies show that the complex and nanocomplex could bind with BSA. The results of synchronous fluorescence of BSA show that additions of the complex affect the microenvironment of both tyrosine and tryptophan residues during the binding process. The in vitro cytotoxicity of the complex (solution in DMSO) and nanocomplex (colloid in H2O) against the human carcinoma cell lines (MCF-7 and A-549) was evaluated by MTT assay. The results of in vitro cytotoxicity indicate that the complex and nanocomplex have excellent cytotoxicity activity against MCF-7 and A-549. Results of the microscopic analyses of the cancer cells confirm the results of the cytotoxicity.
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http://dx.doi.org/10.1007/s10895-015-1739-2DOI Listing
March 2016

A mononuclear Cu(II) complex with 5,6-diphenyl-3-(2-pyridyl)-1,2,4-triazine: Synthesis, crystal structure, DNA- and BSA-binding, molecular modeling, and anticancer activity against MCF-7, A-549, and HT-29 cell lines.

Eur J Med Chem 2015 9;96:66-82. Epub 2015 Apr 9.

Università degli Studi di Messina, dip. Scienze Chimiche, Viale Ferdinando S. d'Alcontres, 98166 Messina, Italy.

The copper(II) complex of 1,2,4-triazine derivatives, [Cu(dppt)2(H2O)](PF6)2(dppt is 5,6-diphenyl-3-(2-pyridyl)-1,2,4-triazine), has been synthesized and fully characterized by spectroscopic methods and single crystal X-ray diffraction. The in vitro DNA-binding studies of the complex have been investigated by several methods. The results showed that the complex intercalates into the base pairs of DNA. The complex also indicated good binding propensity to BSA. The results of molecular docking and molecular dynamic simulation methods confirm the experimental results. Finally, the in vitro cytotoxicity indicate that the complex has excellent anticancer activity against the three human carcinoma cell lines, MCF-7, A-549, and HT-29, with IC50 values of 9.8, 7.80, and 4.50 μM, respectively. The microscopic analyses of the cancer cells demonstrate that the Cu(II) complex apparently induced apoptosis.
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http://dx.doi.org/10.1016/j.ejmech.2015.04.020DOI Listing
February 2016

Antioxidant and cytotoxic effect of biologically synthesized selenium nanoparticles in comparison to selenium dioxide.

J Trace Elem Med Biol 2014 Jan 1;28(1):75-9. Epub 2013 Aug 1.

Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran. Electronic address:

The present study was designed to evaluate antioxidant and cytotoxic effect of selenium nanoparticles (Se NPs) biosynthesized by a newly isolated marine bacterial strain Bacillus sp. MSh-1. An organic-aqueous partitioning system was applied for purification of the biogenic Se NPs and the purified Se NPs were then investigated for antioxidant activity using DPPH scavenging activity and reducing power assay. Cytotoxic effect of the biogenic Se NPs and selenium dioxide (SeO2) on MCF-7 cell line was assesed by MTT assay. Tranmission electron micrograph (TEM) of the purified Se NPs showed individual and spherical nanostructure in size range of about 80-220nm. The obtained results showed that, at the same concentration of 200μg/mL, Se NPs and SeO2 represented scavenging activity of 23.1±3.4% and 13.2±3.1%, respectively. However, the data obtained from reducing power assay revealed higher electron-donating activity of SeO2 compared to Se NPs. Higher IC50 of the Se NPs (41.5±0.9μg/mL) compared to SeO2 (6.7±0.8μg/mL) confirmed lower cytotoxicity of the biogenic Se NPs on MCF-7 cell line.
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http://dx.doi.org/10.1016/j.jtemb.2013.07.005DOI Listing
January 2014