Publications by authors named "Maharshi Panchal"

3 Publications

  • Page 1 of 1

A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience.

Authors:
Cory J Evans John M Olson Bama Charan Mondal Pratyush Kandimalla Ariano Abbasi Mai M Abdusamad Osvaldo Acosta Julia A Ainsworth Haris M Akram Ralph B Albert Elitzander Alegria-Leal Kai Y Alexander Angelica C Ayala Nataliya S Balashova Rebecca M Barber Harmanjit Bassi Sean P Bennion Miriam Beyder Kush V Bhatt Chinmay Bhoot Aaron W Bradshaw Tierney G Brannigan Boyu Cao Yancey Y Cashell Timothy Chai Alex W Chan Carissa Chan Inho Chang Jonathan Chang Michael T Chang Patrick W Chang Stephen Chang Neel Chari Alexander J Chassiakos Iris E Chen Vivian K Chen Zheying Chen Marsha R Cheng Mimi Chiang Vivian Chiu Sharon Choi Jun Ho Chung Liset Contreras Edgar Corona Courtney J Cruz Renae L Cruz Jefferson M Dang Suhas P Dasari Justin R O De La Fuente Oscar M A Del Rio Emily R Dennis Petros S Dertsakyan Ipsita Dey Rachel S Distler Zhiqiao Dong Leah C Dorman Mark A Douglass Allysen B Ehresman Ivy H Fu Andrea Fua Sean M Full Arash Ghaffari-Rafi Asmar Abdul Ghani Bosco Giap Sonia Gill Zafar S Gill Nicholas J Gills Sindhuja Godavarthi Talin Golnazarian Raghav Goyal Ricardo Gray Alexander M Grunfeld Kelly M Gu Natalia C Gutierrez An N Ha Iman Hamid Ashley Hanson Celesti Hao Chongbin He Mengshi He Joshua P Hedtke Ysrael K Hernandez Hnin Hlaing Faith A Hobby Karen Hoi Ashley C Hope Sahra M Hosseinian Alice Hsu Jennifer Hsueh Eileen Hu Spencer S Hu Stephanie Huang Wilson Huang Melanie Huynh Carmen Javier Na Eun Jeon Sunjong Ji Jasmin Johal Amala John Lauren Johnson Saurin Kadakia Namrata Kakade Sarah Kamel Ravinder Kaur Jagteshwar S Khatra Jeffrey A Kho Caleb Kim Emily Jin-Kyung Kim Hee Jong Kim Hyun Wook Kim Jin Hee Kim Seong Ah Kim Woo Kyeom Kim Brian Kit Cindy La Jonathan Lai Vivian Lam Nguyen Khoi Le Chi Ju Lee Dana Lee Dong Yeon Lee James Lee Jason Lee Jessica Lee Ju-Yeon Lee Sharon Lee Terrence C Lee Victoria Lee Amber J Li Jialing Li Alexandra M Libro Irvin C Lien Mia Lim Jeffrey M Lin Connie Y Liu Steven C Liu Irene Louie Shijia W Lu William Y Luo Tiffany Luu Josef T Madrigal Yishan Mai Darron I Miya Mina Mohammadi Sayonika Mohanta Tebogo Mokwena Tonatiuh Montoya Dallas L Mould Mark R Murata Janani Muthaiya Seethim Naicker Mallory R Neebe Amy Ngo Duy Q Ngo Jamie A Ngo Anh T Nguyen Huy C X Nguyen Rina H Nguyen Thao T T Nguyen Vincent T Nguyen Kevin Nishida Seo-Kyung Oh Kristen M Omi Mary C Onglatco Guadalupe Ortega Almazan Jahzeel Paguntalan Maharshi Panchal Stephanie Pang Harin B Parikh Purvi D Patel Trisha H Patel Julia E Petersen Steven Pham Tien M Phan-Everson Megha Pokhriyal Davis W Popovich Adam T Quaal Karl Querubin Anabel Resendiz Nadezhda Riabkova Fred Rong Sarah Salarkia Nateli Sama Elaine Sang David A Sanville Emily R Schoen Zhouyang Shen Ken Siangchin Gabrielle Sibal Garuem Sin Jasmine Sjarif Christopher J Smith Annisa N Soeboer Cristian Sosa Derek Spitters Bryan Stender Chloe C Su Jenny Summapund Beatrice J Sun Christine Sutanto Jaime S Tan Nguon L Tan Parich Tangmatitam Cindy K Trac Conny Tran Daniel Tran Duy Tran Vina Tran Patrick A Truong Brandon L Tsai Pei-Hua Tsai C Kimberly Tsui Jackson K Uriu Sanan Venkatesh Maique Vo Nhat-Thi Vo Phuong Vo Timothy C Voros Yuan Wan Eric Wang Jeffrey Wang Michael K Wang Yuxuan Wang Siman Wei Matthew N Wilson Daniel Wong Elliott Wu Hanning Xing Jason P Xu Sahar Yaftaly Kimberly Yan Evan Yang Rebecca Yang Tony Yao Patricia Yeo Vivian Yip Puja Yogi Gloria Chin Young Maggie M Yung Alexander Zai Christine Zhang Xiao X Zhang Zijun Zhao Raymond Zhou Ziqi Zhou Mona Abutouk Brian Aguirre Chon Ao Alexis Baranoff Angad Beniwal Zijie Cai Ryan Chan Kenneth Chang Chien Umar Chaudhary Patrick Chin Praptee Chowdhury Jamlah Dalie Eric Y Du Alec Estrada Erwin Feng Monica Ghaly Rose Graf Eduardo Hernandez Kevin Herrera Vivien W Ho Kaitlyn Honeychurch Yurianna Hou Jo M Huang Momoko Ishii Nicholas James Gah-Eun Jang Daphne Jin Jesse Juarez Ayse Elif Kesaf Sat Kartar Khalsa Hannah Kim Jenna Kovsky Chak Lon Kuang Shraddha Kumar Gloria Lam Ceejay Lee Grace Lee Li Li Joshua Lin Josephine Liu Janice Ly Austin Ma Hannah Markovic Cristian Medina Jonelle Mungcal Bilguudei Naranbaatar Kayla Patel Lauren Petersen Amanda Phan Malcolm Phung Nadiyah Priasti Nancy Ruano Tanveer Salim Kristen Schnell Paras Shah Jinhua Shen Nathan Stutzman Alisa Sukhina Rayna Tian Andrea Vega-Loza Joyce Wang Jun Wang Rina Watanabe Brandon Wei Lillian Xie Jessica Ye Jeffrey Zhao Jill Zimmerman Colton Bracken Jason Capili Andrew Char Michel Chen Pingdi Huang Sena Ji Emily Kim Kenneth Kim Julie Ko Sean Louise G Laput Sam Law Sang Kuk Lee Olivia Lee David Lim Eric Lin Kyle Marik Josh Mytych Andie O'Laughlin Jensen Pak Claire Park Ruth Ryu Ashwin Shinde Manny Sosa Nick Waite Mane Williams Richard Wong Jocelyn Woo Jonathan Woo Vishaal Yepuri Dorothy Yim Dan Huynh Dinali Wijiewarnasurya Casey Shapiro Marc Levis-Fitzgerald Leslie Jaworski David Lopatto Ira E Clark Tracy Johnson Utpal Banerjee

G3 (Bethesda) 2021 Jan;11(1)

Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.

Undergraduate students participating in the UCLA Undergraduate Research Consortium for Functional Genomics (URCFG) have conducted a two-phased screen using RNA interference (RNAi) in combination with fluorescent reporter proteins to identify genes important for hematopoiesis in Drosophila. This screen disrupted the function of approximately 3500 genes and identified 137 candidate genes for which loss of function leads to observable changes in the hematopoietic development. Targeting RNAi to maturing, progenitor, and regulatory cell types identified key subsets that either limit or promote blood cell maturation. Bioinformatic analysis reveals gene enrichment in several previously uncharacterized areas, including RNA processing and export and vesicular trafficking. Lastly, the participation of students in this course-based undergraduate research experience (CURE) correlated with increased learning gains across several areas, as well as increased STEM retention, indicating that authentic, student-driven research in the form of a CURE represents an impactful and enriching pedagogical approach.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/g3journal/jkaa028DOI Listing
January 2021

Cyclooxygenase inhibition targets neurons to prevent early behavioural decline in Alzheimer's disease model mice.

Brain 2016 07 13;139(Pt 7):2063-81. Epub 2016 May 13.

1 Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA

Identifying preventive targets for Alzheimer's disease is a central challenge of modern medicine. Non-steroidal anti-inflammatory drugs, which inhibit the cyclooxygenase enzymes COX-1 and COX-2, reduce the risk of developing Alzheimer's disease in normal ageing populations. This preventive effect coincides with an extended preclinical phase that spans years to decades before onset of cognitive decline. In the brain, COX-2 is induced in neurons in response to excitatory synaptic activity and in glial cells in response to inflammation. To identify mechanisms underlying prevention of cognitive decline by anti-inflammatory drugs, we first identified an early object memory deficit in APPSwe-PS1ΔE9 mice that preceded previously identified spatial memory deficits in this model. We modelled prevention of this memory deficit with ibuprofen, and found that ibuprofen prevented memory impairment without producing any measurable changes in amyloid-β accumulation or glial inflammation. Instead, ibuprofen modulated hippocampal gene expression in pathways involved in neuronal plasticity and increased levels of norepinephrine and dopamine. The gene most highly downregulated by ibuprofen was neuronal tryptophan 2,3-dioxygenase (Tdo2), which encodes an enzyme that metabolizes tryptophan to kynurenine. TDO2 expression was increased by neuronal COX-2 activity, and overexpression of hippocampal TDO2 produced behavioural deficits. Moreover, pharmacological TDO2 inhibition prevented behavioural deficits in APPSwe-PS1ΔE9 mice. Taken together, these data demonstrate broad effects of cyclooxygenase inhibition on multiple neuronal pathways that counteract the neurotoxic effects of early accumulating amyloid-β oligomers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/brain/aww117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4939702PMC
July 2016

Prostaglandin signaling suppresses beneficial microglial function in Alzheimer's disease models.

J Clin Invest 2015 Jan 8;125(1):350-64. Epub 2014 Dec 8.

Microglia, the innate immune cells of the CNS, perform critical inflammatory and noninflammatory functions that maintain normal neural function. For example, microglia clear misfolded proteins, elaborate trophic factors, and regulate and terminate toxic inflammation. In Alzheimer's disease (AD), however, beneficial microglial functions become impaired, accelerating synaptic and neuronal loss. Better understanding of the molecular mechanisms that contribute to microglial dysfunction is an important objective for identifying potential strategies to delay progression to AD. The inflammatory cyclooxygenase/prostaglandin E2 (COX/PGE2) pathway has been implicated in preclinical AD development, both in human epidemiology studies and in transgenic rodent models of AD. Here, we evaluated murine models that recapitulate microglial responses to Aβ peptides and determined that microglia-specific deletion of the gene encoding the PGE2 receptor EP2 restores microglial chemotaxis and Aβ clearance, suppresses toxic inflammation, increases cytoprotective insulin-like growth factor 1 (IGF1) signaling, and prevents synaptic injury and memory deficits. Our findings indicate that EP2 signaling suppresses beneficial microglia functions that falter during AD development and suggest that inhibition of the COX/PGE2/EP2 immune pathway has potential as a strategy to restore healthy microglial function and prevent progression to AD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1172/JCI77487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382270PMC
January 2015