Publications by authors named "Mabel Mariela Rodríguez Cordeiro"

15 Publications

  • Page 1 of 1

PLGA+HA/βTCP scaffold incorporating simvastatin: a promising biomaterial for bone tissue engineering.

J Oral Implantol 2020 Jul 22. Epub 2020 Jul 22.

DDS, MSc, PhD, professor in the Department of Dentistry, Federal University of Santa Catarina, Brazil.

The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) + hydroxyapatite / β-tricalcium phosphate (PLGA+HA/βTCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% βTCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m:m) of this medication. Scaffolds were synthesized in a cylindric-shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in PBS at 37 °C under constant stirring for 7, 14, 21, and 28 days. Non-degraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro, meso, and micropores in the polymer structure with HA/βTCP particles homogeneously dispersed. Chemical and thermal analyses presented very similar results for both PLGA+HA/βTCP and PLGA+HA/βTCP+SIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/βTCP and PLGA+HA/βTCP+SIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/βTCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1563/aaid-joi-D-19-00148DOI Listing
July 2020

Retrospective study of 124 cases of salivary gland tumors and literature review.

J Clin Exp Dent 2019 Nov 1;11(11):e1025-e1032. Epub 2019 Nov 1.

Department of Pathology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

Background: Salivary gland tumors are a rare and morphologically diverse group of lesions and their frequency is still unknown in several parts of the world. The knowledge of its population characteristics contributes to a better understanding of its etiopathogenesis. Objectives: This study investigated the frequency of salivary gland tumors in a region of southern Brazil and compared these data in a literature review.

Material And Methods: A retrospective study was conducted of salivary gland tumors diagnosed at two pathology centers from 1995 to 2016. Patient age and gender, tumor site and frequency, histopathological diagnosis, and symptomatology were evaluated. Chi-squared tests were used to assess the associations between variables. To compare our data, we also conducted a literature review of publications in the PubMed and LILACS databases of retrospective studies of salivary gland tumors.

Results: A total of 124 salivary gland tumor cases was identified, 81 (65.3%) of which were classified as benign and 43 (34.6%) as malignant. Most tumors occurred in the parotid gland (57.2%). Pleomorphic adenoma was the most common tumor (59.6%), followed by adenocarcinoma not otherwise specified (8.8%). The tumors occurred more often in women (54.8%) than in men (45.2%). Malignant tumors were associated with pain in 31.4% of cases (<0.05). The literature review included 35 articles from different countries. Women were most affected, with a mean age of 41.7 years. The most common benign tumor was pleomorphic adenoma (48.2%) and the most common malignant tumor was mucoepidermoid carcinoma (8.7%).

Conclusions: The results of the present study showed that salivary gland tumors are rare. The parotid gland is the most common location and pleomorphic adenoma are the most frequent lesions. The malignant tumors presented as several histological types and the incidence was variable globally. Salivary gland neoplasms, salivary gland diseases, oral surgery, epidemiology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4317/jced.55685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825733PMC
November 2019

Inhibition of cancer stem cells promoted by Pimozide.

Clin Exp Pharmacol Physiol 2019 02 28;46(2):116-125. Epub 2018 Nov 28.

Department of Morphological Sciences, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.

Over the past years, studies have described that users of antipsychotics are less likely to develop cancer than the population in general due to cytotoxic properties of this class of drugs on cancer cells. For this reason, Pimozide has been widely studied as a potential anticancer treatment, and satisfactory results in melanoma, central nervous system tumours, osteosarcoma, neuroblastoma, myeloproliferative neoplasms, breast, lung, prostate, ovarian, colorectal, pancreatic, and hepatocellular carcinoma have been showed. Moreover, advantages as clinical use approved by the Food and Drug Administration (FDA), high clinical safety, low side effects, and reasonable price have stimulated the treatment with Pimozide instead of other agents. The action mechanism remains unclear, but three vias associated to cancer stem cell (CSC) hypothesis show that Pimozide: (a) blocks CSC features, as epithelial-to-mesenchymal transition (EMT), through inhibition of Wnt-β/catenin signalling; (b) acts as an inhibitor of signal transducer and activator of transcription (STAT-3 and 5), pathway which is activated and up-regulated in CSCs; (c) inhibits ubiquitine specific protease (USP1) and WD repeat-containing protein 48 (WDR48), that are proteins responsible to inhibit the differentiation and to maintain the cell in an undifferentiated state. Based on this perspective, the aim of this manuscript is to review the antineoplastic role of Pimozide during tumorigenesis and its potential to revert the process of undifferentiation and proliferation of CSC through different vias.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1440-1681.13049DOI Listing
February 2019

Expression of Cancer Stem Cell Biomarkers in Human Head and Neck Carcinomas: a Systematic Review.

Stem Cell Rev Rep 2018 Dec;14(6):769-784

Department of Morphological Sciences, Health Sciences Center, Federal University of Santa Catarina, Trindade, Florianópolis, Santa Catarina, Brazil.

Malignant neoplasms may be composed of several cell groups, including cancer stem cells (CSC). These cells have been related with the capacity of metastasis, relapse and resistance to multiple drugs during chemotherapy. This study aims to identify CSC biomarkers and their expression pattern in human head and neck carcinomas. This study was conducted following the PRISMA checklist. The search for articles was carried out in five databases (PubMed, Scopus, Web of Science, Lilacs and Scielo). The articles found were selected in two phases: 1) reading the titles and / or abstract and 2) reading the full text. At the end, the selected articles were evaluated by QUADAS-2. Most studies evaluated oral neoplastic tissues and, as a control, samples of normal local mucosa. All studies performed immunohistochemistry as a method of immunolocalization and some also applied immunofluorescence. The most commonly used biomarker was CD44. However, other such as Sox2, Oct4, Nestin, Nanog, BMI1, ALDH1, CD133 and CD166 were also found. Several biomarkers were (ALDH1, Sox2, Oct4, ABCB5, AGR2 and TAZ) correlated with clinical characteristics of the tumor, such as staging, tumor size and lymph node metastasis. These data reinforce the CSC theory and favor the use of these biomarkers as possible determinants of prognosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12015-018-9839-4DOI Listing
December 2018

Crown discoloration promoted by materials used in regenerative endodontic procedures and effect of dental bleaching: spectrophotometric analysis.

J Appl Oral Sci 2017 Mar-Apr;25(2):234-242

Universidade Federal de Santa Catarina, Departamento de Odontologia, Florianópolis, Santa Catarina, Brasil.

Objectives: To assess tooth crown's color after intracanal treatment with triple antibiotic paste (TAP) or calcium hydroxide (CH); cervical sealing with glass ionomer cement (GIC) or mineral trioxide aggregate (MTA); and bleaching with carbamide peroxide.

Material And Methods: After pulp removal and color spectrophotometer measurement, 50 bovine incisors were divided into 4 experimental groups and one control (untreated). Experiments were performed in phases (Ph). Ph1: TAP (ciprofloxacin, metronidazole, minocycline), TAPM (ciprofloxacin, metronidazole, amoxicillin), DAP (ciprofloxacin, metronidazole), or CH treatment groups. After 1 and 3 days (d); 1, 2, 3 weeks (w); and 1, 2, 3 and 4 months (m), color was measured and medications were removed. Ph2: GIC or MTA cervical sealing, each using half of the specimens from each group. Color was assessed after 1d, 3d; 1w, 2w, 3w; 1m and 2m. Ph3: Two bleaching sessions, each followed by color measurement. Data were analyzed with ANOVA and post-hoc Holm-Sidak method.

Results: Ph1: Specimens of TAP group presented higher color alteration (ΔE) mean than those of TAPM group. No significant difference was found among TAP or TAPM and CH, DAP or Control groups. Ph2: cervical sealing materials showed no influence on color alteration. Ph3: Different ΔE means (from different groups), prior to bleaching, became equivalent after one bleaching session.

Conclusions: TAP induces higher color alteration than TAPM; color alteration increases over time; cervical sealing material has no influence on color alteration; and, dental bleaching was able to recover, at least partially, the tooth crown's color.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1590/1678-77572016-0398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393545PMC
May 2017

The Role of the Complex USP1/WDR48 in Differentiation and Proliferation Processes in Cancer Stem Cells.

Curr Stem Cell Res Ther 2017 ;12(5):416-422

Department of Pathology, Federal University of Santa Catarina, Reitor Joao David Ferreira Lima Campus, Zip Code 88040-900, Florianopolis, Santa Catarina. Brazil.

Background: Recently, some studies identified the Basic-Helix-Loop-Helix (bHLH) transcription factor as a significant regulator for the evolution of neoplasms. The binding between bHLH proteins and DNA is restricted by heterodimerization with Inhibitors of DNA binding (ID). IDs prevent cellular differentiation, promote growth and sustain tumor development. The wide presence of stem cells in cancers suggests that genes ID are essential to cancer stem cells (CSC) progress. The enzyme Ubiquitin-specific protease 1 (USP1) is reported to deubiquitinate and stabilize IDs. Considering the action of the proteins ID, USP1 contributes to prevent differentiation mediated by bHLH and, consequently, keep CSC original characteristics. USP1 has its activity potentiated when bound to protein WD repeat-containing protein (WDR48).

Objective: To identify the influence of the complex USP1/WDR48 during the CSC tumorigenesis process, and whether this complex is a possible therapeutic target.

Methods: A literature search regarding the role of the complex USP1/WDR48 in inhibiting differentiation and increasing proliferation of CSC was performed, and possible selective molecule inhibitors of these deubiquitinase proteins were investigated.

Results: There is evidence that USP1/WDR48 complex promotes stem cell conservation and regulation of DNA damage repair. For this reason, inhibitors as Pimozide, GW7647, C527, SJB2-043, ML323 have been studied to inhibit USPs in cases of treatment intervention.

Conclusion: It is consolidated in the literature the role of USP1/WDR48 during tumorigenesis. However, these studies are not enough to completely clarify the process; but certainly, the researchers are converging towards a promising direction to provide a new treatment option for cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2174/1574888X12666170315104013DOI Listing
April 2018

Medication-Related Osteonecrosis of Jaws: A Low-Level Laser Therapy and Antimicrobial Photodynamic Therapy Case Approach.

Case Rep Dent 2016 7;2016:6267406. Epub 2016 Sep 7.

Department of Pathology, Ambulatory of Stomatology, University Hospital, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

Medication-related osteonecrosis of the jaws (MRONJ) can be considered an inability of the alveolar bone to respond to an injury, which frequently leads to severe local and systemic complications. Once the problem is installed, dentist must use all therapeutic approaches recommended. This manuscript reports a successful management of MRONJ handled with antibiotics, conservative debridement, low-level laser therapy (LLLT), and photodynamic therapy (PDT) up to 12 months. As healing of MRONJ may be very slow, combined therapeutic approaches are required. Besides the recommended conventional treatment protocol, LLLT and PDT are important tools to contribute to healing and improvement of patient's quality of life.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2016/6267406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030412PMC
September 2016

Immunohistochemical expression of matrix metalloproteinases in ameloblastomas and pericoronal follicles.

J Oral Pathol Med 2016 Sep 23;45(8):586-90. Epub 2015 Dec 23.

Department of Pathology, Health Sciences Center, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil.

Background: Ameloblastoma (AM) is a benign odontogenic neoplasm characterized by local invasiveness and recurrence. We compared the immunohistochemical expression of matrix metalloproteinases in different clinical types of AM as well as in normal odontogenic tissue.

Methods: Thirteen cases of solid AMs, five cases of unicystic AM and eight pericoronal follicles (PF) were selected and subjected to immunohistochemical investigation for matrix metalloproteinase-1, matrix metalloproteinase-2 and matrix metalloproteinase-9 expressions.

Results: The expressions of MMP-1 and MMP-2 were very high in the cytoplasm of cells throughout the entire epithelium and in fibroblasts from the adjacent connective tissue. MMP-9 expression was observed in the same location although with weaker staining. The Kruskal-Wallis test showed statistically significant differences in the epithelial expressions of MMP-1 and MMP-2; there was lower expression among solid AMs when compared with unicystic AM and PF. Compared to both types of AM, higher stromal expression of MMP-9 was found in PF.

Conclusion: MMP-1, MMP-2 and MMP-9 seem to be associated with AM tumour behaviour as well as physiological tissue remodelling within PF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jop.12411DOI Listing
September 2016

Analysis of Bone Repair and Inflammatory Process Caused by Simvastatin Combined With PLGA+HA+βTCP Scaffold.

Implant Dent 2016 Feb;25(1):140-8

*PhD Student, Department of Dentistry, Implantology, Federal University of Santa Catarina, Florianópolis, SC, Brazil. †PhD Student, Department of Physiological Sciences, Neuroscience, Federal University of Santa Catarina, Florianópolis, SC, Brazil. ‡Professor, Department of Physiological Sciences, Neuroscience, Federal University of Santa Catarina, Florianópolis, SC, Brazil. §Professor, Department of Dentistry, Radiology, Federal University of Santa Catarina, Florianópolis, SC, Brazil. ¶Professor, Department of Public Health, Public Health, Federal University of Santa Catarina, Florianópolis, SC, Brazil. ‖PostDoctoral, Department of Stomatology, Implantology, Federal University of Santa Catarina, Florianópolis, SC, Brazil. #Professor, Department of Morphological Sciences, Histology, Federal University of Santa Catarina, Florianópolis, SC, Brazil. **Professor, Department of Morphological Sciences, Anatomy, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

Purpose: This study evaluated the tissue and inflammatory responses to the use of simvastatin and poly(lactic-co-glycolic acid) + hydroxyapatite + β-tricalcium phosphate (PLGA+HA+βTCP) scaffold for bone repair.

Materials And Methods: Two defects of 5 mm in diameter were made in the calvaria of rats, which were shared into the following 6 groups: naive, sham, vehicle, PLGA+HA+βTCP scaffold, simvastatin (4 mg/mL), and simvastatin with the scaffold. Tissue samples were collected at 1, 7, 15, 30, and 60 days after surgery. Inflammation was evaluated by interleukin-1 beta and tumor necrosis factor alpha quantification and by a hemogram, whereas bone repair was evaluated using densitometry and scanning electron microscopy. Data were statistically analyzed using ANOVA followed by post hoc tests (P < 0.05).

Results: There was an increased cytokine expression in the scaffold and simvastatin groups (P < 0.001 and P < 0.05, respectively) 1 day after surgery but no alterations on the hemogram were observed. It was found on bone tissue samples that 60 days after surgery all groups presented similar densitometry values and morphology characteristics, despite the occurrence of bone formation delay in the simvastatin group (P < 0.01).

Conclusion: The use of simvastatin and PLGA+HA+βTCP scaffold, associated or not, did not lead to improvement in bone repair.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/ID.0000000000000359DOI Listing
February 2016

Microbial reduction by two chemical-mechanical protocols in primary teeth with pulp necrosis and periradicular lesion - an in vivo study.

Braz Dent J 2014 ;25(4):307-13

Department of Morphological Sciences, UFSC - Federal University of Santa Catarina, Florianópolis, SC, Brazil.

The objective of this study was to determine the efficacy of chemical-mechanical procedures of two endodontic protocols for septic content reduction of root canals from primary teeth with pulp necrosis and periradicular lesion. Twenty-four primary root canals with pulp necrosis and periradicular lesion were divided into two treatment groups (n=12): multiple-visit and single-visit protocols. Samples were collected using sterile paper points before and after endodontic cleaning followed by microbiological identification through checkerboard DNA-DNA hybridization. Statistical analysis was performed using Proportion Test for score=0 comparing the findings before and after treatment for each group (Wilcoxon's test) as well as the differences in scores between protocols (Mann-Whitney's test) (p<0.05). Data were expressed as prevalence (presence or absence) and estimate of the average count (x10(5) cells) of each species. Differences in proportions of score=0 prior to treatment were non-significant (p=0.415), demonstrating equivalence between groups. A significant increase in score=0 was detected after treatment for both groups (p<0.0001). Single-visit protocol achieved a significantly greater reduction in mean scoring following endodontic treatment (p=0.024). Both protocols were capable of significantly reducing septic content in root canals of primary teeth with periradicular lesion. Moreover, single-visit protocol showed greater efficacy in reducing endodontic infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1590/0103-6440201302416DOI Listing
August 2016

Dentigerous cyst of inflammatory origin.

J Dent Child (Chic) 2014 May-Aug;81(2):112-6

Department of Pathology, São Thiago University Hospital, at the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.

There is an association between persistent, prolonged inflammation of a primary tooth and the development of an inflammatory dentigerous cyst involving the succedaneous tooth. The purpose of this case report is to describe the management of an inflammatory dentigerous cyst of the permanent maxillary left central incisor in a nine-year-old boy caused by a long-term inflammation/infection of its predecessor. The treatment consisted of conservative decompression, which allowed for rapid healing and the eruption of the permanent tooth. The patient was followed up with periodic clinical and radiographic evaluations for several years.
View Article and Find Full Text PDF

Download full-text PDF

Source
May 2015

Microbial profile of root canals of primary teeth with pulp necrosis and periradicular lesion.

J Dent Child (Chic) 2014 Jan-Apr;81(1):14-9

Department of Morphological Sciences, at the School of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.

Purpose: The purpose of this study was to assess the microbial content of root canals of human primary teeth with pulp necrosis and periradicular lesion.

Methods: Microbial samples were collected from 24 canals of children treated at a pediatric dentistry clinic. Microbiological identification was performed using checker-board DNA-DNA hybridization for 40 different bacteria. Data were analyzed per canal based on the mean count and frequency of each bacterial species.

Results: Detectable levels of bacterial species were observed for 35 probes (88%). The most frequent bacteria were Fusobacterium nucleatum sp. nucleatum, Fusobacterium periodonticum, Prevotella melaninogenica, Prevotella nigrescens, and Prevotella intermedia. Facultative species were identified in 20 root canals (83%), anaerobic species were identified in 24 root canals (100%), and aerobic species in 18 root canals (75%). Black-pigmented bacilli were found in 23 samples (96%). The number of different bacterial species detected per canal ranged from five to 33.

Conclusion: Endodontic infection in primary teeth with pulp necrosis and periradicular lesion is multimicrobial, including aerobic, facultative, and anaerobic micro-organisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
December 2014

Pattern of fluoride-containing dentifrice use and associated factors in preschool children from Ijuí, South Brazil.

Oral Health Prev Dent 2010 ;8(3):277-85

Department of Paediatric Dentistry, School of Dentistry, Lutheran University of Brazil, Porto Alegre-RS 90 035 121, Brazil.

Purpose: The aim of the present study was to evaluate the pattern of fluoride-containing dentifrice use and associated factors in Brazilian preschoolers.

Materials And Methods: A structured questionnaire was answered by parents of 432 children, who were aged 2 to 6 years, from 12 public preschools in Ijuí, South Brazil. Primary outcomes were age of starting fluoride-containing dentifrice use and amount of dentifrice applied to the toothbrush. Poisson regression with robust variance was used to determine the factors associated with the main outcomes.

Results: About 75% of the participants were introduced to fluoride-containing dentifrice before the age of 2 years. The current predominant amount of dentifrice used was obtained by transversal (57.7%), followed by longitudinal deposition on the toothbrush (33.6%), corresponding to 0.3 and 0.9 g of dentifrice, respectively. Previous dental visits and maternal education greater than 4 years were associated with the introduction of fluoride-containing dentifrice before 2 years of age. The probability of using dentifrice in amounts higher than the recommendations doubled in children aged 5 and 6 years.

Conclusions: Oral health interventions should reinforce the importance of early introduction of fluoride-containing dentifrice, especially in less-educated mothers. Furthermore, the amount of dentifrice should be reduced to maximise the caries-preventive effect while minimising dental fluorosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
January 2011

Ex vivo study of manual and rotary instrumentation techniques in human primary teeth.

Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008 Apr;105(4):e84-92

Brazil School of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina.

Objective: This study aimed to evaluate, ex vivo, time and dentin removal by manual and rotary instrumentation in human primary teeth.

Study Design: Eighty primary teeth were divided into 2 groups, embedded in resin, and sectioned for evaluation before and after instrumentation. For manual technique, teeth were prepared using K-type files and rotary preparation was performed with Hero 642. All root canals were prepared up to size 40, and the instrumentation time was recorded. Dentin removal was calculated using digital image software. Data was statistically analyzed using chi-squared, Mann-Whitney, and t tests.

Results: Manual instrumentation removed more dentin in all groups compared with rotary instrumentation (P < .05). In some specimens, root perforations were observed in areas coinciding with largest root resorption. Rotary technique showed the shortest instrumentation time (P < .05).

Conclusions: Deciduous teeth, without significant root resorption, have enough root structure for instrumentation up to size 40. Rotary technique required less time and generated less dentin removal.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tripleo.2007.12.008DOI Listing
April 2008

The effects of periradicular inflamation and infection on a primary tooth and permanent successor.

J Clin Pediatr Dent 2005 ;29(3):193-200

Primary teeth and the permanent successors must be understood as interdependent units, where each one of them interacts with and depends on each other. Pulpal inflammation/infection of a primary tooth and the spread of this condition over the periradicular tissues can lead to alterations in the dental germ of the permanent successor and to the surrounding structures if no therapy is done, i.e. endodontics or extraction. This work will present cases of permanent teeth that showed alteration in eruption and / or in development, as a consequence of inflammation / infection of the preceding primary teeth, such as: hypoplasia, morphological alteration on the dental crown or total arrest of. radicular formation. The teeth analysed in this study belong to patients who attended the Universidade Federal de Santa Catarina Children's Dentistry Clinic. The earlier these lesions are diagnosed, the less were the destructive effects and the consequences on the primary tooth/permanent germ unit.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.17796/jcpd.29.3.5238p10v21r2j162DOI Listing
September 2005