Publications by authors named "M Zubair Tahir"

1,336 Publications

  • Page 1 of 1

Taste improvement of Maillard reaction intermediates derived from enzymatic hydrolysates of pea protein.

Food Res Int 2021 Feb 16;140:109985. Epub 2020 Dec 16.

Department of Food Science, Rutgers University, New Brunswick, NJ 08901, USA. Electronic address:

Maillard reaction intermediates (MRIs) derived from enzymatic hydrolysates of pea protein, mainly initial Maillard reaction products, were prepared at a low temperature (80 °C) and the reaction time was determined by variable-temperature Maillard reaction. Electronic tongue and sensory evaluation were used to analyze the taste qualities of pea protein hydrolysates and their MRIs. Both evaluations showed that bitterness of enzymatic hydrolysates of pea protein reduced but umami taste increased through Maillard reaction. The intensities of umami and saltiness were positively correlated with the concentration of MRIs. Even when the dosage of MRIs was 0.1% (w/w), MRIs could achieve a 20% reduction in NaCl content without decreasing saltiness, which could be great potential substitutes for salt reduction. On the other hand, the increased MRIs promoted aldosterone secretion in saliva, which might enhance human perception of saltiness.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodres.2020.109985DOI Listing
February 2021

Knowledge, Attitudes, and Practices of the Pakistani Population Regarding Facemasks During the COVID-19 Pandemic.

Asia Pac J Public Health 2021 Mar 1:1010539521997261. Epub 2021 Mar 1.

Dow University of Health Sciences, Karachi, Pakistan.

The main aim of this study was to evaluate the knowledge, attitudes, and practices of the public regarding mask use during the COVID-19 (coronavirus disease 2019) pandemic. In this online survey, a questionnaire designed according to the World Health Organization's recommendations for mask use and its method of use was administered to 426 Pakistani respondents. Only one third (33.1%) had adequate knowledge of mask use, more than two thirds (71.1%) had positive attitude to masks, and only less than half (43.0%) respondents had good practices regarding masks and preventive measures. Massive input from the governing bodies is needed to combat the threat of coronavirus.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1010539521997261DOI Listing
March 2021

Designing Online Grocery Stores to Support Healthy Eating for Weight Loss.

Public Health Nutr 2021 Feb 26:1-33. Epub 2021 Feb 26.

Division of Epidemiology and Community Health, School of Public Health, University of Minnesota, 1300 South 2nd St., Suite 300, Minneapolis, MN55454.

Objectives: This study aimed to identify features to include in online grocery stores to support healthful food purchasing by those striving to lose weight.

Design: A Value Proposition Design Approach was used to gain shopper insights, devise potential online grocery store features, and obtain feedback on these features.

Setting: Telephone interviews were conducted to gain insight into shoppers' needs and perceptions. Results were used by the research team to identify potential online grocery shopping features that may support healthful purchase decisions, and interviews were conducted with a different sample of shoppers to gather feedback on features.

Participants: Insight (n=25) and feedback (n=25) interviews were conducted with convenience samples of adults trying to lose weight.

Results: Participants were primarily female, white, college educated, and with obesity or overweight. Online grocery features devised by the research team based on findings from the insight interviews included a: 1) shopping cart nutrition rating tool; 2) healthy meal planning tool; 3) interactive healthy eating inspiration aisle; and 4) healthy shopping preference settings option. Findings from the feedback interviews indicated that the healthy meal planning tool, healthy shopping preference settings option and shopping cart nutrition rating tool features were positively rated by most participants.

Conclusions: There are multiple features grocers should consider including in their online stores to attract and support customers striving to eat healthy for weight loss.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1017/S1368980021000896DOI Listing
February 2021

A High-Performance Asymmetric Supercapacitor Based on Tungsten Oxide Nanoplates and Highly Reduced Graphene Oxide Electrodes.

Chemistry 2021 Feb 20. Epub 2021 Feb 20.

Johannes Gutenberg University: Johannes Gutenberg Universitat Mainz, Chemistry, GERMANY.

Tungsten oxide/graphene hybrid-materials are attractive semiconductors for energy-related applications. Herein, we report an asymmetric supercapacitor (ASC, HRG//m-WO 3 ASC), fabricated from monoclinic tungsten oxide (m-WO 3 ) nanoplates as a negative-electrode and highly reduced graphene oxide (HRG) as a positive-electrode material. The supercapacitor performance of the prepared electrodes was evaluated in an aqueous-electrolyte (1M H 2 SO 4 ) using three- and two- electrode systems. The HRG//m-WO 3 ASC exhibits a maximum specific capacitance of 389 F/g at a current density of 0.5 A/g, with an associated high energy density of 93 Wh/kg at a power density of 500 W/kg in a wide 1.6 V operating potential window. In addition, the HRG//m-WO 3 ASC displays long-term cycling-stability, maintaining ~92% of the original specific capacitance after 5000 galvanostatic charge-discharge cycles. The m-WO 3 nanoplates were prepared hydrothermally while HRG was synthesized by a modified Hummers method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/chem.202005156DOI Listing
February 2021

Transcatheter aortic valve replacement same-day discharge for selected patients: a case series.

Eur Heart J Case Rep 2021 Feb 12;5(2):ytaa556. Epub 2021 Jan 12.

Department of Cardiology, Sands Constellation Heart Institute, Rochester Regional Health, 1425 Portland Avenue, Rochester, NY 14621, USA.

Background: The coronavirus disease 2019 (COVID-19) pandemic has resulted in drastic changes to the practice of medicine, requiring healthcare systems to find solutions to reduce the risk of infection. Using a case series, we propose a protocol for same-day discharge (SDD) for selected patients undergoing transcatheter aortic valve replacement (TAVR) using real-time remote cardiac monitoring. Six patients with severe symptomatic aortic stenosis underwent TAVR and were discharged on the same day.

Case Summary: Six patients with symptomatic severe native or bioprosthetic aortic valve stenosis underwent a successful transfemoral TAVR using standard procedures, including the use of rapid atrial pacing to assess the need for permanent pacemaker implantation. Following TAVR, patients were monitored on telemetry in the recovery area for 3 h, ambulated to assess vascular access stability, and discharged with real-time remote cardiac monitoring if no new conduction abnormality was observed. The patients were seen by tele-visits within 2 days and 2 weeks after discharge.

Discussion: Amidst the COVID-19 pandemic, SDD following successful transfemoral TAVR may be feasible for selected patients and reduce potential COVID-19 exposure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/ehjcr/ytaa556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873812PMC
February 2021

In-situ growth of TiO imbedded TiCT nanosheets to construct PCN/TiCT MXenes 2D/3D heterojunction for efficient solar driven photocatalytic CO reduction towards CO and CH production.

J Colloid Interface Sci 2021 Feb 2;591:20-37. Epub 2021 Feb 2.

Chemical Reaction Engineering Group (GREG), School of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310, UTM, Johor Bahru, Johor, Malaysia.

Constructing efficient structured materials for artificial photosynthesis of CO is a promising strategy to produce renewable fuels in addition of mitigating greenhouse effect. In this work, 2D porous g-CN (PCN) coupled exfoliated 3D TiCT MXene (TiC) nanosheets with TiO NPs in-situ growth was constructed in a single step through HF treatment approach. The different exfoliated TiC structures were successfully synthesized for adjusting HF etching time (24 h, 48 h and 96 h). With growing etchant time from 24 to 96 h, the amount of TiO produced was increased, but it has adverse effects on CO and CH production rate. The maximum production rates for CO and CH of 317.4 and 78.55 µmol g h were attained when the 10TiC-48/PCN was employed than using TiC-24/PCN, TiC-96/PCN and PCN composite samples, respectively. The performance of 10TiC-48/PCN composite for CO and CH evolution were 9.9 and 6.7 folds higher than using pristine PCN sample, respectively. The possible mechanism is assigned to porous structure with intimate contact enabling efficient charge carrier separation with the role of TiO NPs to work as a bridge to transport electrons towards MXene surface. Among the reducing agents, water was favorable for CO evolution, whereas, methanol-water system promoted CH production. All these findings confirm that heterojunction formation facilitates charges separation and can be further used in solar energy relating application.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.01.099DOI Listing
February 2021

Cd bioavailability and nitrogen cycling microbes interaction affected by mixed amendments under paddy-pak choi continued planting.

Environ Pollut 2021 Feb 6;275:116542. Epub 2021 Feb 6.

Department of Soil, Water, & Climate, Univ. of Minnesota, 1991 Upper Buford Cir, Falcon Heights, MN, 55108, USA.

Cadmium (Cd) is the most concerning soil pollutant, and a threat to human health, especially in China. The in-situ immobilization of Cadmium by amendments is one of the most widely adopted methods to remedy soil contamination. The study was designed to evaluate the effect of organo-chemical amendments on soil Cd bioavailability and nitrogen cycling microbes under continuous planting of rice (Oryza sativa) and pak choi (Brassica chinensis L.). The experiment was carried out using four amendments, Lime, Zeolite, Superphosphate, and Biochar, at two different ratios; M1: at the ratio of 47:47:5:1, and M2 at the ratio of 71:23:5:1, respectively. Moreover, both M1 and M2 were enriched at four levels (T1: 0.5%; T2: 1%; T3: 2%; T4: 4%). Results showed that compared with CK (Cd enriched soils), the yield of rice under treatments of M1T1 and M2T1 increased by 8.93% and 8.36%, respectively. While the biomass (fresh weight) of pak choi under M1 and M2 amendments increased by 2.52-2.98 times and 0.76-2.89 times respectively, under enrichment treatments T1, T2, and T3. The total Cd concentrations in rice grains treated with M1T3 and M2T3 decreased by 89.25% and 93.16%, respectively, compared with CK. On the other hand, the total Cd concentrations in pak choi under M1T3 and M2T2 decreased by 92.86% and 90.23%, respectively. The results showed that soil pH was the main factor affecting Cd bioavailability in rice and pak choi. The Variance partitioning analysis (VPA) of rice and pak choi showed that soil pH was the most significant contributing factor. In the rice season, the contribution of soil pH (P) on Cd bioavailability was 10.14% (P = 0.102), and in the pak choi season, the contribution of soil pH was 8.38% (P = 0.133). Furthermore, the abundance of ammonia oxidation and denitrifying microorganisms had significantly correlation with soil pH and exchange Cd. In rice season, when the enrichment level of amendments increased from 0.5% (T1) to 2% (T3), the gene abundance of AOA, AOB, nirK, nirS and nosZ (І) tended to decrease. While in pak choi season, when the enrichment level increased at the level of 0.5% (T1), 1% (T2), and 2% (T3), the gene abundance of AOB, nirS, and nosZ (І) increased. Additionally, the gene abundance of AOA and nirK showed a reduction in the pak choi season contrasting to rice. And the mixed amendment M2 performed better at reducing Cd uptake than M1, which may have correlation with the ratio of lime and zeolite in them. Finally, we conclude that between these two amendments, when applied at a moderate level M2 type performed better than M1 in reducing Cd uptake, and also showed positive effects on both gene abundance and increase soil pH.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2021.116542DOI Listing
February 2021

Application of Tourniquet Does Not Influence Early Clinical Outcomes After Total Knee Arthroplasty.

Cureus 2021 Jan 2;13(1):e12435. Epub 2021 Jan 2.

Spine Surgery, Royal National Orthopaedic Hospital, London, GBR.

Introduction The use of a tourniquet during total knee arthroplasty (TKA) is still a topic of debate, given the conflicting results in the literature with respect to complications, pain, functional outcome, and blood loss. However, due to a lack of convincing data on early patient-reported outcomes (PROMS), this study aims to compare pain and functional outcomes in total knee arthroplasty patients with and without a tourniquet. Methods A randomized controlled trial was set up at a tertiary care hospital that spanned from 1 February 2015 to 31 July 2018. We included all primary total knee arthroplasties performed for patients aged between 50 and 80 years. Oxford Knee Score (OKS), Numerical Pain Rating Score (NPRS), Visual Analogue Scale (VAS) for satisfaction, active range of knee motion, and Short Form-12 Survey (SF-12) scores were collected pre-surgery and then at six-weeks and six-months interval with a p-value of 0.05 considered to be significant. Results Two hundred and forty patients participated in the study; 117 patients were randomized to surgery with the tourniquet inflated and 123 to surgery with the tourniquet deflated. There were 43.4% males, and 56.6% females in the tourniquet inflated group with an average age of 62.29±9.63 years while in tourniquet deflated group, there were 46.7% males and 53.3% females with a mean age of 65.41±9.042 years (p-value for age is 0.404; the p-value for gender is 0.086).  Despite the increase in intraoperative blood loss in both the groups, there was no significant increase in blood transfusions as both groups recorded the need for postoperative blood transfusion - 12 patients in the tourniquet group and 19 in the non-tourniquet group, but this difference was statistically insignificant (p=0.231). The perioperative blood loss was significantly lower (p<0.001) in the tourniquet group (490.29±47.752) compared to in the non-tourniquet group (526.18±12.796), while the duration of surgery was comparable in both groups (p=0.156). The length of stay for the two groups did not statistically differ (p=0.976) - the mean length of stay for the tourniquet group was 6.16±2.38 days and for the non-tourniquet group it was 6.18±2.34 days. There were no significant differences between the two groups regarding patient-reported outcomes (PROMS) at six-weeks and six-months. However, during the in-hospital stay, only the NPRS score for knee pain showed that the non-tourniquet patients had a lower NPRS compared to the tourniquet group and this difference was statistically significant (p=0.02). During the postoperative hospital stay, there was no significant difference among the two treatment groups for VAS, OKS, SF-12, and range of motion (flexion/extension). At the six weeks follow-up, both groups had similar outcomes for the range of movements and pain scores. Besides, no difference was noted among the tourniquet and non-tourniquet groups even after a follow-up of six months. Regarding complications, 27 patients in the tourniquet group did complain of numbness during the study period compared to 10 in the non-tourniquet group (p=0.001). Conclusion In conclusion, a tourniquet application helps minimize intraoperative blood loss and results in a faster procedure. Furthermore, the application of the tourniquet is safe and effective and does not affect the functional outcomes and pain scale in total knee arthroplasty.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7759/cureus.12435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852345PMC
January 2021

A quality improvement project to assess and refine the handover process at morning trauma meetings.

Ann Med Surg (Lond) 2021 Feb 7;62:406-414. Epub 2021 Jan 7.

Trauma and Orthopaedics Department, Sandwell Hospital, SWBH NHS Trust, United Kingdom.

Background: Poor handover and inadequate transmission of clinical information between shifts can result in patient harm. This study was designed to evaluate the impact of implementing a handover protocol on the quality of information exchanged in the trauma handover meetings in a UK district general hospital.

Methods: A prospective single centre observational study was performed at an acute NHS trust, using the define, measure, analyse, improve and control (DMAIC) methodology. Ten consecutive weekday trauma meetings, involving 43 patients, were observed to identify poor practices in handover. This data was used in conjunction with the Royal College of Surgeon's recommendations for effective handover (2007) to create a standard operating protocol (SOP). Following the implementation of the SOP, a further eight consecutive weekday trauma meetings, involving a further 47 patients, were observed. The data collection was performed by five trained independent observers. The data was analysed using -test for quantitative variables and chi-square or Fisher's exact tests for categorical variables.

Results: An improvement in the trauma handover was demonstrated in multiple aspects of trauma handover including patient's past medical history, date of injury, results, diagnosis, consent, mark and starvation status (all p < 0.001). Subgroup analyses showed that handover of neck of femur patients including information on baseline mobility (p = 0.04), Nottingham Hip Fracture Score (p = 0.01), next of kin discussion (p = 0.075) and resuscitation status (p = 0.001) all improved following our interventions.

Conclusion: These results demonstrate that the implementation of a well-structured handover protocol can improve the transmission of critical information in trauma meetings.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.amsu.2020.12.046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858682PMC
February 2021

Improving the management of type 2 diabetes through large-scale general practice: the role of a data-driven and technology-enabled education programme.

BMJ Open Qual 2021 Feb;10(1)

AT Medics Ltd, London, London, UK

A quality improvement (QI) scheme was launched in 2017, covering a large group of 25 general practices working with a deprived registered population. The aim was to improve the measurable quality of care in a population where type 2 diabetes (T2D) care had previously proved challenging. A complex set of QI interventions were co-designed by a team of primary care clinicians and educationalists and managers. These interventions included organisation-wide goal setting, using a data-driven approach, ensuring staff engagement, implementing an educational programme for pharmacists, facilitating web-based QI learning at-scale and using methods which ensured sustainability. This programme was used to optimise the management of T2D through improving the eight care processes and three treatment targets which form part of the annual national diabetes audit for patients with T2D. With the implemented improvement interventions, there was significant improvement in all care processes and all treatment targets for patients with diabetes. Achievement of all the eight care processes improved by 46.0% (p<0.001) while achievement of all three treatment targets improved by 13.5% (p<0.001). The QI programme provides an example of a data-driven large-scale multicomponent intervention delivered in primary care in ethnically diverse and socially deprived areas.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/bmjoq-2020-001087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7871240PMC
February 2021

Mechanistic new insights of flavonols on neurodegenerative diseases.

Biomed Pharmacother 2021 Feb 2;137:111253. Epub 2021 Feb 2.

The Key Laboratory of High-Altitude Medical Application of Qinghai Province, Qinghai, Xining, 810001, China; Department of Basic Medicine, Medical College of Qinghai University, Qinghai, Xining, 810001, China. Electronic address:

With a large and increasing elderly population, neurodegenerative diseases such as Parkinson's disease (PD), Huntington disease (HD), Alzheimer's disease (AD), Amyotrophic lateral sclerosis (ALS) and Multiple sclerosis (MS) have become a major and growing health problem. During the past few decades, the elderly population has grown 2.5 % every year. Unfortunately, there are no specific therapeutic remedies available to slow the onset or development of these diseases. An aging brain causes many pathophysiological changes and is the major risk factor for most of the neurodegenerative disorders. Polyphenolic compounds such as flavonols have shown therapeutic potential and can contribute to the treatment of these diseases. In this review, evidence for the beneficial neuroprotective effect of multiple flavonols is discussed and their multifactorial cellular pathways for the progressions of age-associated brain changes are identified. Moreover, the animal models of these diseases support the neuroprotective effect and target the potential of flavonols in the treatment of neurodegenerative diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2021.111253DOI Listing
February 2021

National Trends of Sex Disparity in the American College of Cardiology/American Heart Association Guideline Writing Committee Authors Over 15 Years.

Circ Cardiovasc Qual Outcomes 2021 Feb 5;14(2):e007578. Epub 2021 Feb 5.

Division of Cardiology, University of Arizona, Phoenix (M.G.).

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1161/CIRCOUTCOMES.120.007578DOI Listing
February 2021

Knowledge and practices: Risk perceptions of COVID-19 and satisfaction with preventive measures at workplace among maternity care providers in Pakistan.

Eur J Midwifery 2021 29;5. Epub 2021 Jan 29.

Aga Khan Hospital for Women, Karachi, Pakistan.

Introduction: While all healthcare services across the globe deferred non-urgent surgeries, labor wards provided maternity care during the COVID-19 pandemic continuously. This study assesses the knowledge and practices of obstetricians and midwives about personal protective equipment (PPE); their risk perception of COVID-19 and satisfaction with the preventive measures adopted at their workplace.

Methods: A questionnaire designed according to the World Health Organization’s advice on rational use of personal protective equipment for COVID-19 was administered to 452 Pakistani maternity care providers between 1 July and 30 July 2020.

Results: Most (85%) had adequate knowledge and 78.8% had good practices regarding PPE use. The perceived risk of contracting COVID-19 was lower than for influenza and tuberculosis. Perceived risk of contracting COVID-19 was highest for outpatient clinics. Fewer midwives compared to obstetricians (23.3% vs 32.9 %, p=0.001) were satisfied with the job security provided. Only 19.5% were satisfied with the social distancing measures at their setups. Less than one-third (31%) were satisfied with the PPE available to them.

Conclusions: The participants had good knowledge and practices regarding PPE. The perceived risk of contracting COVID-19 was lower than for contracting influenza; however, they were concerned about contracting COVID-19 in outpatient clinics and emergency rooms. They had poor satisfaction with the measures adopted by hospital managements regarding job security and social distancing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18332/ejm/131864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847223PMC
January 2021

Aging and Allostasis: Using Bayesian Network Analytics to Explore and Evaluate Allostatic Markers in the Context of Aging.

Diagnostics (Basel) 2021 Jan 21;11(2). Epub 2021 Jan 21.

The Physical Activity and Nutrition INfluences In Ageing (PANINI) Consortium: School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.

Allostatic load reflects the cumulative strain on organic functions that may gradually evolve into overt disease. Our aim was to evaluate the allostatic parameters in the context of aging, and identify the parameters that may be suitable for an allostatic load index for elderly people (>60 years). From previously published studies, 11 allostatic (bio)markers could be identified that sustain sufficient variability with aging to capture meaningful changes in health status. Based on reported statistics (prevalence of a biomarker and its associated outcome, and/or an odds/risk ratio relating these two), seven of these could be adopted in a Bayesian Belief Network (BBN), providing the probability of "disturbed" allostasis in any given elder. Additional statistical analyses showed that changes in IL-6 and BMI contributed the most to a "disturbed" allostasis, indicating their prognostic potential in relation to deteriorating health in otherwise generally healthy elderly. In this way, and despite the natural decline in variance that irrevocably alters the prognostic relevance of most allostatic (bio)markers with aging, it appeared possible to outline an allostatic load index specifically for the elderly. The allostatic parameters here identified might consequently be considered a useful basis for future quantitative modelling in the context of (healthy) aging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/diagnostics11020157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912325PMC
January 2021

Plaster immobilization versus anterior plating for dorsally displaced distal radial fractures in elderly patients in Pakistan.

J Hand Surg Eur Vol 2021 Jan 24:1753193420977780. Epub 2021 Jan 24.

Department of Orthopaedics, Leeds Teaching Hospital Trust, Leeds, UK.

This prospective, multi-centred, randomized trial examined outcomes of 3- and 12-month follow-ups of 159 elderly participants aged more than 75 years with isolated distal radial fractures, treated by anterior locking plate or closed reduction and cast immobilization. The primary outcome was the patient-rated wrist evaluation (PRWE) score. The PRWE score at 12 months was not significantly different between the two groups; however, the radiological outcomes and complications rates were worse in the casting group. III.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1753193420977780DOI Listing
January 2021

Ankle Fracture Fixation with Use of WALANT (Wide Awake Local Anesthesia with No Tourniquet) Technique: An Attractive Alternative for the Austere Environment.

J Bone Joint Surg Am 2021 Mar;103(5):397-404

Department of Orthopaedics, Sirsyed Medical College, Karachi, Pakistan.

Background: Wide awake local anesthesia with no tourniquet (WALANT) is a simple technique that has had successful application in hand surgery. When utilized in the plating of distal radial fractures, the WALANT technique has proven to be a cost-effective approach in orthopaedic trauma. The aim of the present study was to assess the applicability of the WALANT technique in open reduction and internal fixation of ankle fractures.

Methods: Fifty-eight patients were recruited from October 2016 to September 2018. Fractures were categorized according to the OTA/AO classification. We did not include patients with Maisonneuve, talar, or posterior malleolar fractures. Mechanism of injury, fracture type, comorbidities, and complications were recorded. The surgical site was infiltrated to achieve local anesthesia using a solution of 0.9% normal saline solution and 2% lidocaine with 1:1,000,000 epinephrine. Functional outcomes were assessed at the time of fracture union with use of the American Orthopaedic Foot & Ankle Society (AOFAS) Ankle-Hindfoot Scale.

Results: Thirty-nine patients (67%) were male and 19 (33%) were female, and the mean age was 46.79 ± 8.54 years. According to the OTA/AO classification, 36 fractures (62%) were 44C2, 11 (19%) were 44B2, 7 (12%) were 44C1, and 4 (7%) were 44B1 fractures. Average blood loss was 29.40 ± 7.38 mL, the mean operative time was 59.72 ± 7.19 minutes, and the mean intraoperative visual analogue scale (VAS) for pain was 1.24 ± 0.43 (range, 0 to 3). The mean time to union was 16.21 ± 3.52 weeks, with a mean AOFAS Ankle-Hindfoot Scale score of 86.38 ± 5.72. Three patients (5.2%) developed nonunion of a fracture site, 2 patients had a surgical site infection, and 1 noncompliant diabetic patient developed osteomyelitis.

Conclusions: The WALANT technique is a safe technique for ankle fracture fixation and has been shown to be useful in foot and ankle surgery, particularly in limited-resource environments.

Level Of Evidence: Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2106/JBJS.20.00196DOI Listing
March 2021

Rare case of right ventricular dilatation associated with anomalous pulmonary venous drainage, sinus venosus atrial septal defect and persistent left superior vena cava.

BMJ Case Rep 2021 Jan 11;14(1). Epub 2021 Jan 11.

Cardiology, Betsi Cadwaladr University Health Board, Rhyl, UK.

A 34-year-old woman was seen in the emergency department for shortness of breath and chest pain. During a pandemic, it is easy to 'think horses and not zebras', and with a patient presenting with the classic coronavirus symptoms it would have been easy to jump to that as her diagnosis. After a careful history and examination, it became clear that there was another underlying diagnosis. Chest X-ray, echocardiogram and CT scan revealed marked right ventricular dilatation and pulmonary hypertension, alongside a persistent left superior vena cava (PLSVC). Further investigation with cardiac MRI and coronary angiography at a tertiary centre demonstrated that she not only have a PLSVC but also a partial anomalous pulmonary venous drainage and sinus venosus atrial septal defect. This case highlights the importance of considering all differentials and approaching investigations in a logical manner.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/bcr-2020-239687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802694PMC
January 2021

Attitudes of nurses and physicians toward nurse-physician interprofessional collaboration in different hospitals of Islamabad-Rawalpindi Region of Pakistan.

J Interprof Care 2021 Jan 11:1-6. Epub 2021 Jan 11.

Final Year Medical Student, Shifa College of Medicine, Shifa Tameer-e-Millat University , Islamabad, Pakistan.

Interprofessional collaboration (IPC) is crucial to efficient patient management in the modern healthcare setting. We sought to determine the attitudes of physicians and nurses working in different hospitals in the Islamabad-Rawalpindi region of Pakistan. We employed the Jefferson Scale of Attitudes toward Physician-Nurse Collaboration (JSAPNC), a 15-item questionnaire that quantifies these attitudes in a meaningful way. Higher scores indicate a more positive attitude toward IPC. Four domains (shared education and teamwork, caring vs. curing, nurses' autonomy, and physicians' dominance) represent the intricate factors that influence IPC in a hospital setting. A total of 374 healthcare professionals responded. Nurses had significantly better opinions about IPC compared to physicians (mean: 50.81 vs. 47.48, <.01). Nurses also outscored physicians in all four domains of the JSAPNC (education and collaboration 24.87 vs. 23.72 <.001, caring vs. curing 10.88 vs. 9.42 <.001, nurse's autonomy 10.89 vs. 10.51 =.004, and physician's authority 4.17 vs. 3.82 =.044). The results show that nurses in Pakistan value IPC more than their physician colleagues. Inculcating the importance of IPC through educational methods might help improve these attitudes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/13561820.2020.1853079DOI Listing
January 2021

An overview of genetic mutations and epigenetic signatures in the course of pancreatic cancer progression.

Cancer Metastasis Rev 2021 Mar 10;40(1):245-272. Epub 2021 Jan 10.

College of Life Science and Bioengineering, Beijing University of Technology, 100 Ping Le Yuan, Chaoyang, Beijing, 100124, China.

Pancreatic cancer (PC) is assumed to be an intimidating and deadly malignancy due to being the leading cause of cancer-led mortality, predominantly affecting males of older age. The overall (5 years) survival rate of PC is less than 9% and is anticipated to be aggravated in the future due to the lack of molecular acquaintance and diagnostic tools for its early detection. Multiple factors are involved in the course of PC development, including genetics, cigarette smoking, alcohol, family history, and aberrant epigenetic signatures of the epigenome. In this review, we will mainly focus on the genetic mutations and epigenetic signature of PC. Multiple tumor suppressor and oncogene mutations are involved in PC initiation, including K-RAS, p53, CDKN2A, and SMAD4. The mutational frequency of these genes ranges from 50 to 98% in PC. The nature of mutation diagnosis is mostly homozygous deletion, point mutation, and aberrant methylation. In addition to genetic modification, epigenetic alterations particularly aberrant hypermethylation and hypomethylation also predispose patients to PC. Hypermethylation is mostly involved in the downregulation of tumor suppressor genes and leads to PC, while multiple genes also represent a hypomethylation status in PC. Several renewable drugs and detection tools have been developed to cope with this aggressive malady, but all are futile, and surgical resection remains the only choice for prolonged survival if diagnosed before metastasis. However, the available therapeutic development is insufficient to cure PC. Therefore, novel approaches are a prerequisite to elucidating the genetic and epigenetic mechanisms underlying PC progression for healthier lifelong survival.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10555-020-09952-0DOI Listing
March 2021

Genome-wide identification of the 14-3-3 gene family and its participation in floral transition by interacting with TFL1/FT in apple.

BMC Genomics 2021 Jan 8;22(1):41. Epub 2021 Jan 8.

College of Horticulture, Northwest A & F University, Yangling, 712100, China.

Background: Apple (Malus domestica Borkh.) is a popular cultivated fruit crop with high economic value in China. Apple floral transition is an important process but liable to be affected by various environmental factors. The 14-3-3 proteins are involved in regulating diverse biological processes in plants, and some 14-3-3 members play vital roles in flowering. However, little information was available about the 14-3-3 members in apple.

Results: In the current study, we identified eighteen 14-3-3 gene family members from the apple genome database, designated MdGF14a to MdGF14r. The isoforms possess a conserved core region comprising nine antiparallel α-helices and divergent N and C termini. According to their structural and phylogenetic features, Md14-3-3 proteins could be classified into two major evolutionary branches, the epsilon (ɛ) group and the non-epsilon (non-ɛ) group. Moreover, expression profiles derived from transcriptome data and quantitative real-time reverse transcription PCR analysis showed diverse expression patterns of Md14-3-3 genes in various tissues and in response to different sugars and hormone treatments during the floral transition phase. Four Md14-3-3 isoforms (MdGF14a, MdGF14d, MdGF14i, and MdGF14j) exhibiting prominent transcriptional responses to sugars and hormones were selected for further investigation. Furthermore, yeast two-hybrid and bimolecular fluorescence complementation experiments showed that the four Md14-3-3 proteins interact with key floral integrators, MdTFL1 (TERMINAL FLOWER1) and MdFT (FLOWERING LOCUS T). Subcellular localization of four selected Md14-3-3 proteins demonstrated their localization in both the cytoplasm and nucleus.

Conclusion: We identified the Md14-3-3 s family in apple comprehensively. Certain Md14-3-3 genes are expressed predominantly during the apple floral transition stage, and may participate in the regulation of flowering through association with flower control genes. Our results provide a preliminary framework for further investigation into the roles of Md14-3-3 s in floral transition.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12864-020-07330-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796649PMC
January 2021

Use of Hyperspectral Reflectance Sensing for Assessing Growth and Chlorophyll Content of Spring Wheat Grown under Simulated Saline Field Conditions.

Plants (Basel) 2021 Jan 6;10(1). Epub 2021 Jan 6.

Department of Biology, College of Science and Humanities at Quwayiyah, Shaqra University, Riyadh 19257, Saudi Arabia.

The application of proximal hyperspectral sensing, using simple vegetation indices, offers an easy, fast, and non-destructive approach for assessing various plant variables related to salinity tolerance. Because most existing indices are site- and species-specific, published indices must be further validated when they are applied to other conditions and abiotic stress. This study compared the performance of various published and newly constructed indices, which differ in algorithm forms and wavelength combinations, for remotely assessing the shoot dry weight (SDW) as well as chlorophyll a (), chlorophyll b (), and chlorophyll a+b () content of two wheat genotypes exposed to three salinity levels. Stepwise multiple linear regression (SMLR) was used to extract the most influential indices within each spectral reflectance index (SRI) type. Linear regression based on influential indices was applied to predict plant variables in distinct conditions (genotypes, salinity levels, and seasons). The results show that salinity levels, genotypes, and their interaction had significant effects ( ≤ 0.05 and 0.01) on all plant variables and nearly all indices. Almost all indices within each SRI type performed favorably in estimating the plant variables under both salinity levels (6.0 and 12.0 dS m) and for the salt-sensitive genotype Sakha 61. The most effective indices extracted from each SRI type by SMLR explained 60%-81% of the total variability in four plant variables. The various predictive models provided a more accurate estimation of and content than of SDW and under both salinity levels. They also provided a more accurate estimation of SDW than of content for salt-tolerant genotype Sakha 93, exhibited strong performance for predicting the four variables for Sakha 61, and failed to predict any variables under control and for Sakha 93. The overall results indicate that the simple form of indices can be used in practice to remotely assess the growth and chlorophyll content of distinct wheat genotypes under saline field conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/plants10010101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825289PMC
January 2021

A new virus of the family Tombusviridae infecting sugarcane.

Arch Virol 2021 Mar 7;166(3):961-965. Epub 2021 Jan 7.

USDA ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA.

Many viral diseases of sugarcane negatively affect yield. A sugarcane accession originating from South Africa exhibiting mosaic symptoms was processed for high-throughput sequencing. Bioinformatic analysis revealed two known sugarcane viruses and a contig of around 2,800 nucleotides resembling umbra-like viruses of the family Tombusviridae. The sequence of the viral contig was confirmed by cloning and Sanger sequencing, and the ends of the virus sequence were determined. Open reading frame analysis revealed the presence of four ORFs. Phylogenetic analysis of the complete virus sequence showed that this virus clusters with other umbra-like viruses of the family Tombusviridae.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00705-020-04908-9DOI Listing
March 2021

COVID-19: A Global Challenge with Old History, Epidemiology and Progress So Far.

Molecules 2020 Dec 23;26(1). Epub 2020 Dec 23.

Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh 202002, UP, India.

Humans have witnessed three deadly pandemics so far in the twenty-first century which are associated with novel coronaviruses: SARS, Middle East respiratory syndrome (MERS), and COVID-19. All of these viruses, which are responsible for causing acute respiratory tract infections (ARTIs), are highly contagious in nature and/or have caused high mortalities. The recently emerged COVID-19 disease is a highly transmittable viral infection caused by another zoonotic novel coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Similar to the other two coronaviruses such as SARS-CoV-1 and MERS-CoV, SARS-CoV-2 is also likely to have originated from bats, which have been serving as established reservoirs for various pathogenic coronaviruses. Although, it is still unknown how SARS-CoV-2 is transmitted from bats to humans, the rapid human-to-human transmission has been confirmed widely. The disease first appeared in Wuhan, China, in December 2019 and quickly spread across the globe, infected 48,539,872 people, and caused 1,232,791 deaths in 215 countries, and the infection is still spreading at the time of manuscript preparation. So far, there is no definite line of treatment which has been approved or vaccine which is available. However, different types of potential vaccines and therapeutics have been evaluated and/or are under clinical trials against COVID-19. In this review, we summarize different types of acute respiratory diseases and briefly discuss earlier outbreaks of coronaviruses and compare their occurrence and pathogenicity with the current COVID-19 pandemic. Various epidemiological aspects of COVID-19 such as mode of spread, death rate, doubling time, etc., have been discussed in detail. Apart from this, different technical issues related to the COVID-19 pandemic including use of masks and other socio-economic problems associated with the pandemic have also been summarized. Additionally, we have reviewed various aspects of patient management strategies including mechanism of action, available diagnostic tools, etc., and also discussed different strategies for the development of effective vaccines and therapeutic combinations to deal with this viral outbreak. Overall, by the inclusion of various references, this review covers, in detail, the most important aspects of the COVID-19 pandemic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules26010039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795815PMC
December 2020

Characterisation at the Bonding Zone between Fly Ash Based Geopolymer Repair Materials (GRM) and Ordinary Portland Cement Concrete (OPCC).

Materials (Basel) 2020 Dec 24;14(1). Epub 2020 Dec 24.

Department of Physics, Częstochowa University of Technology, 42-200 Częstochowa, Poland.

In recent years, research and development of geopolymers has gained significant interest in the fields of repairs and restoration. This paper investigates the application of a geopolymer as a repair material by implementation of high-calcium fly ash (FA) as a main precursor, activated by a sodium hydroxide and sodium silicate solution. Three methods of concrete substrate surface preparation were cast and patched: as-cast against ordinary Portland cement concrete (OPCC), with drilled holes, wire-brushed, and left as-cast against the OPCC grade 30. This study indicated that FA-based geopolymer repair materials (GRMs) possessed very high bonding strength at early stages and that the behavior was not affected significantly by high surface treatment roughness. In addition, the investigations using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) spectroscopy have revealed that the geopolymer repair material became chemically bonded to the OPC concrete substrate, due to the formation of a C-A-S-H gel. Fundamentally, the geopolymer network is composed of tetrahedral anions (SiO) and (AlO) sharing the oxygen, which requires positive ions such as Na, K, Li, Ca, Na, Ba, NH, and HO. The availability of calcium hydroxide (Ca(OH)) at the surface of the OPCC substrate, which was rich in calcium ions (Ca), reacted with the geopolymer; this compensated the electron vacancies of the framework cavities at the bonding zone between the GRM and the OPCC substrate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma14010056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795290PMC
December 2020

Case Report: as a Cause of Chronic Meningitis.

Front Neurol 2020 8;11:601242. Epub 2020 Dec 8.

Division of Infectious Diseases, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, United States.

is closely related to and rarely isolated in clinical specimens. is increasingly recognized as a pathogen in immunocompromised hosts. We present the third known case of meningitis in a young immunocompetent host. A 27-year-old female with a history of intravenous heroin use and chronic hepatitis C presented with a 10-month history of headaches and progressive bilateral vision loss. On physical examination, visual acuity was 20/20 in her right eye and grade II papilledema was noted. Examination of her left eye revealed complete loss of vision and grade IV papilledema. An MRI with and without contrast revealed increased leptomeningeal enhancement involving the posterior fossa and spinal cord. After multiple lumbar punctures, cerebrospinal fluid fungal cultures grew . The patient was successfully treated with a combination of liposomal amphotericin and fluconazole for 6 weeks with complete resolution of her CNS symptoms, with the exception of irreversible vision loss. We report a case of chronic meningitis due to in an immunocompetent woman with hepatitis C and a history of intravenous heroin use. Additional studies are needed to confirm risk factors for colonization, which likely predisposes individuals to invasive candidiasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2020.601242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7752766PMC
December 2020

Synthesis, Characterization, and Studies of Novel Spirooxindole Derivatives as Ecto-5'-Nucleotidase Inhibitors.

ACS Med Chem Lett 2020 Dec 29;11(12):2397-2405. Epub 2020 Oct 29.

Centre for Advanced Drug Research, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, Pakistan.

Ecto-5'-nucleotidase (ecto-5'-NT, CD73) inhibitors are promising drug candidates for cancer therapy. Traditional efforts used to inhibit the ecto-5'-nucleotidase have involved antibody therapy or development of small molecule inhibitors that can mimic the acidic and ionizable structure of adenosine 5'-monophosphate (AMP). Herein, we report an efficient, environment friendly route for the synthesis of non-nucleotide based small molecules, i.e., substituted spirooxindole derivatives - and investigated their inhibitory potential on human and rat recombinant ecto-5'-nucleotidase isozymes. These attempts have resulted in the identification of compound (IC = 0.15 ± 0.02 μM) inhibitor on -ecto-5'-NT which showed 280-fold higher inhibition and compound (IC ± 0.19 ± 0.03 μM) on -ecto-5'-NT with 406-fold enhanced inhibition than reference standard sulfamic acid. Moreover, studies were carried out to assess binding interactions of potent compounds within enzyme active sites and demonstrated excellent correlation with the experimental findings.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsmedchemlett.0c00343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734640PMC
December 2020

Interactions Between Two Invertebrate Pathogens: An Endophytic Fungus and an Externally Applied Bacterium.

Front Microbiol 2020 30;11:522368. Epub 2020 Nov 30.

USDA-ARS, SEFTNRL, Byron, GA, United States.

The members of family Noctuidae exist in diverse environments and many species from this group are of agriculture importance, particularly spp. (Hübner) (Lepidoptera: Noctuidae) is a major pest of many legumes and cereal crops. Due to environmental and regulatory concerns, safe alternatives to broad spectrum chemical insecticides are needed for the control of key noctuid pests such as . A strain of (Cordycipitaceae: Hypocreales) was evaluated for its ability to colonize endophytically in chickpea plants, and its effectiveness against second (L2) and fourth (L4) larval instars of . was inoculated to chickpea plants through injection and endophytic establishment was confirmed by re-isolating the fungi from leaf samples. A detached leaf assay was used to evaluate pathogenicity. was also applied to both larval stages through leaf dip method. In a novel approach, combined treatments of bacteria and endophytic fungi were compared with single-pathogen treatments. Relative to the single treatments, the combined pathogen treatments exhibited an increase in larval mortality, and decrease in pupation, adult emergence and egg eclosion. Specifically, synergistic effects on mortality were observed when larvae were exposed to simultaneous application of (1 × 10 conidia ml) with (0.75 μg ml). Both instars exhibited varying level of growth, development, frass production, diet consumption and fecundity when exposed to the chickpea leaves inoculated with endophytic and dipped with sub-lethal doses of . These findings indicate that the integrated application of endophytic colonized and can be effectively used against .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2020.522368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734284PMC
November 2020

Does the posterior glenoid osteotomy reduce the rate of recurrence in patients with posterior shoulder instability - A systematic review.

Orthop Traumatol Surg Res 2021 Feb 11;107(1):102760. Epub 2020 Dec 11.

Pan Am Clinic, University of Manitoba, Winnipeg, Manitoba, Canada.

Background: Posterior shoulder instability (PSI) is becoming an increasingly recognised condition. A number of different treatment modalities exist to treat PSI including arthroscopic or open surgeries when non-operative treatment has failed. The primary aim of this systematic review was to analyse the rate of recurrent instability after posterior glenoid osteotomy (PGO) for recurrent PSI, while secondary aim was to identify complication rate and the amount of retroversion correction.

Patients And Methods: A review of the online databases MEDLINE and Embase was conducted on 1 November 2019 according to PRISMA guidelines. The review was registered prospectively in the PROSPERO database (Registration No. CRD42020161984). Clinical studies reporting either the recurrence rate, complications or amount of retroversion correction after PGO for PSI were included. The studies were appraised using the Methodological Index for Non-Randomized Studies (MINORS) tool.

Results: The search strategy identified 9 studies eligible for inclusion. Of the 9 studies, 4 showed an improvement in retroversion with a mean change in retroversion of 10. All 9 studies reported on recurrence rate with an overall rate of 22%. Complications were discussed in only 7 of the studies with overall rate of 18.3%. The most common complication reported in the studies were degenerative changes of the glenohumeral joint (7.3%) and iatrogenic fractures (5.5%).

Conclusion: PGO is a viable option in patients with recurrent PSI that have increased retroversion and have failed non-operative or arthroscopic treatment. It does however carry a significant risk of complications.

Level Of Evidence: IV; Systematic review.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.otsr.2020.102760DOI Listing
February 2021

The validity argument: Addressing the misconceptions.

Med Teach 2020 Dec 11:1-8. Epub 2020 Dec 11.

Department of Basic Clinical Skills, Faculty of Medicine, Sudan International University, Khartoum, Sudan.

Assessment of students necessitates validity. Validity provides strong reasoning for decisions made based on students' scores. It is a unitary concept; the extent to which evidence supports and justify the proposed interpretations of test scores. However, the comprehension of the concept of validity has been associated with some observed misconceptions in the literature. With the consequent impact on the research and application being in consideration, this work aims to address some associated misconceptions to clarify the concept of validity. In doing so, we are adopting the current standard framework that is based on the five sources of validity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/0142159X.2020.1856802DOI Listing
December 2020

Translation and Adaptation of the Diagnostic Criteria for Temporomandibular Disorders into the Malay Language: Psychometric Evaluation of Contents.

J Oral Facial Pain Headache 2020 Fall;34(4):323-330

Aims: To develop the Malay DC/TMD through a formal cross-cultural adaptation (CCA) process for use in non-English speaking populations and to determine the reliability and validity of the Malay Graded Chronic Pain Scale (M-GCPS) and Malay Jaw Functional Limitation Scale (M-JFLS).

Methods: The English DC/TMD was translated into the Malay language using the forward-backward translation procedures specified in the INfORM guideline. The initial Malay instrument was pre-tested, and any discrepancies were identified and reconciled before producing the final Malay DC/TMD. Psychometric properties of the M-GCPS and M-JFLS were evaluated using a convenience sample of 252 subjects and were assessed using internal consistency and test-retest reliability, as well as face, content, concurrent, and construct validity testing. Internal consistency was assessed using Cronbach's alpha, while test-retest reliability was examined using intraclass correlation coefficient (ICC). Concurrent and construct validity of both domains were performed using Spearman ρ correlation test. In addition, construct and discriminant validity were appraised using Kruskal-Wallis and Mann-Whitney U tests, respectively.

Results: Cronbach's alpha values for the M-GCPS and M-JFLS were 0.95 and 0.97, respectively. The ICC was 0.98 for the M-GCPS and 0.99 for M-JFLS. The majority of the tested associations for both domains were found to be statistically significant, with good positive correlations.

Conclusion: The M-GCPS and M-JFLS were found to be reproducible and valid. The Malay DC/TMD shows potential for use among Malay-speaking adults.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.11607/ofph.2624DOI Listing
December 2020