Publications by authors named "M Z Wei"

4,961 Publications

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Design, synthesis and biological evaluation of rhein-piperazine-dithiocarbamate hybrids as potential anticancer agents.

Eur J Med Chem 2022 Aug 5;241:114651. Epub 2022 Aug 5.

Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. Electronic address:

A series of novel rhein-piperazine-dithiocarbamate hybrids 3 were efficiently synthesized from rhein through a catalyst-free and one-pot, three-step sequence involving chlorination and N-acylation followed by dithiocarbamate formation. Hybrids 3 were evaluated for their in vitro cytotoxic potency by MTT assay against several human cancer and non-cancer cells. Five of the hybrids were more cytotoxic to human lung cancer cell line A549 than the parent rhein and the reference, cytarabine (CAR). Structure-activity relationship (SAR) analysis indicated that cytotoxicity was significantly enhanced when ester groups were incorporated into the hybrids (3h-j). In particular, hybrid 3h (IC = 10.93 μg/mL), containing a long-chain alkyl ester, was the most potent compound toward A549 tumor cells, being 7- and 5-fold more toxic than rhein (IC = 77.11 μg/mL) and CAR (IC = 49.27 μg/mL), respectively. Additionally, hybrid 3h was less toxic to the corresponding normal human lung fibroblast cell line, WI-38, with a higher selectivity index (SI, WI-38/A549 ≈ 5) than doxorubicin (DOX, SI ≈ 0), CAR (SI ≈ 2) and rhein (SI ≈ 1). Furthermore, hybrid 3h displayed more toxicity against four types of lung cancer cells (A549, Calu-1, PC-9, and H460; IC = 10.81-23.78 μg/mL) than against six other types of cancer cells (Huh-7, 786-O, HCT116, Hela, SK-BR-3, and SK-OV-3; IC = 23.85-51.98 μg/mL). Further mechanistic studies showed that hybrid 3h induced apoptosis in a concentration-dependent manner in human lung adenocarcinoma cell line PC-9. In vivo safety studies showed that hybrid 3h had no acute toxicity to the major organs of mice and did not lead to blood biochemical index changes. Our results exhibit prominent anti-cancer cell inhibition ability and no obvious systemic toxicity to normal organs, indicating that hybrid 3h has promising potential for further applications in anti-lung cancer drug development.
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http://dx.doi.org/10.1016/j.ejmech.2022.114651DOI Listing
August 2022

Impact of simulating real microplastics on toluene removal from contaminated soil using thermally enhanced air injection.

Environ Pollut 2022 Aug 9:119895. Epub 2022 Aug 9.

State Key Laboratory of Geomechanics and Geotechnical Engineering, Institute of Rock and Soil Mechanics, Chinese Academy of Sciences, Wuhan, 430071, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

This paper investigated the impacts of various real microplastics (MPs), i.e., polyethylene (PE) and polyethylene terephthalate (PET) with different sizes (1000-2000 and 100-200 μm) and different dosages (0.5 and 5% on a dry weight basis), on the toluene removal during the thermally enhanced air injection treatment. First, microscopic tests were carried out to determine the MPs' microstructure and behavior. The PE was mainly a small block, and PET appeared filamentous and sheeted with a larger slenderness ratio. Second, the interactions between MPs and toluene-contaminated soils were revealed by batch adsorption equilibrium experiments and low-field magnetic resonance. The morphological differences and dosage of the MPs impacted soils' total porosity (variation range: 39.2-42.7%) and proportion of the main pores (2-200 μm). Third, the toluene removal during the air injection consisted of compaction, rapid growth, rapid reduction, and tailing stages, and the MPs were regarded as an emerging solid state to affect these removal stages. The final cumulative toluene concentrations of soil-PET mixtures were influenced by total porosity, and those of soil-PE mixtures were controlled by total porosity (influence weight: 0.67) and adsorption capacity (influence weight: 0.33); meanwhile, a self-built comprehensive coefficient of MPs can reflect the relationship between them and cumulative concentrations (correlation coefficient: 0.783).
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http://dx.doi.org/10.1016/j.envpol.2022.119895DOI Listing
August 2022

Chromosome-level and graphic genomes provide insights into metabolism of bioactive metabolites and cold-adaption of Pueraria lobata var. montana.

DNA Res 2022 Aug 12. Epub 2022 Aug 12.

Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.

Pueraria lobata var. montana (P. montana) belongs to the genus Pueraria and originated in Asia. Compared with its sister P. thomsonii, P. montana has stronger growth vigor and cold-adaption, but contains less bioactive metabolites such as puerarin. To promote the investigation of metabolic regulation and genetic improvement of Pueraria, the present study reports a chromosome-level genome of P. montana with length of 978.59 Mb and scaffold N50 of 80.18 Mb. Comparative genomics analysis showed that P. montana possesses smaller genome size than that of P. thomsonii owing to less repeat sequences and duplicated genes. A total of 6,548 and 4,675 variety-specific gene families were identified in P. montana and P. thomsonii, respectively. The identified variety-specific and expanded/contracted gene families related to biosynthesis of bioactive metabolites and microtubules are likely the causes for the different characteristics of metabolism and cold-adaption of P. montana and P. thomsonii. Moreover, a graphic genome was constructed based on 11 P. montana accessions. Total 92 structural variants were identified and most of which are related to stimulus-response. In conclusion, the chromosome-level and graphic genomes of P. montana will not only facilitate the studies of evolution and metabolic regulation, but also promote the breeding of Pueraria.
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http://dx.doi.org/10.1093/dnares/dsac030DOI Listing
August 2022

Vibrationally-Resolved X-ray Photoelectron Spectra of Six Polycyclic Aromatic Hydrocarbons from First-Principles Simulations.

J Phys Chem A 2022 Aug 12. Epub 2022 Aug 12.

MIIT Key Laboratory of Semiconductor Microstructure and Quantum Sensing, Department of Applied Physics, School of Science, Nanjing University of Science and Technology, 210094 Nanjing, China.

Vibrationally resolved C 1s X-ray photoelectron spectra (XPS) of a series of six polycyclic aromatic hydrocarbons (PAHs; phenanthrene, coronene, naphthalene, anthracene, tetracene, and pentacene) were computed by combining the full core hole density functional theory and the Franck-Condon simulations with the inclusion of the Duschinsky rotation effect. Simulated spectra of phenanthrene, coronene, and naphthalene agree well with experiments both in core binding energies (BEs) and profiles, which validate the accuracy of our predictions for the rest molecules with no high-resolution experiments. We found that three types of carbons (inner C), (peripheral C bonded to three C atoms), and (peripheral C bonded to an H atom) show decreasing BEs. In linear PAHs (the latter four), -type carbons further split into 1 or 2 (on inner or edge benzene ring) subtypes with chemical shifts of ca. 0.2-0.4 eV. All major Franck-Condon-active modes are characterized to be in-plane vibrations: low-frequency (<800 cm) C-C ring deformation modes play an essential role in determining the peak asymmetries; and for each -type carbon a high-frequency (ca. 3600 cm) C*-H stretching mode is responsible for the high-energy tail. We found that core ionization leads to reduction of all C*-C and C*-H bond lengths and ring deformation with a definite direction. Based on theoretical spectra of four linear PAHs, we found asymptotic relations and anticipated possible spectral features for even larger linear PAHs. Our calculations provide accurate reference spectra for XPS characterizations of PAHs, which are useful in understanding the vibronic coupling effects in this family.
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http://dx.doi.org/10.1021/acs.jpca.2c04426DOI Listing
August 2022

MITF-Mediated lncRNA CCDC183-As1 Promotes the Tumorigenic Properties and Aerobic Glycolysis of Bladder Cancer via Upregulating TCF7L2.

J Oncol 2022 31;2022:6785956. Epub 2022 Jul 31.

Department of Anesthesiology, The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu, China.

As a primary malignancy tumor of the urology system, bladder cancer (BC) is characterized by its high recurrence and metastasis characteristics. Despite the great improvement in clinical interventions over the past decades, the outcomes of BC patients are still unsatisfactory. Novel molecular mechanisms for developing effective diagnostic and therapeutic strategies are urgently needed; therefore, we screened the lncRNA expression profile in four pairs of BC tissues, showing that CCDC183-AS1 was the most upregulated lncRNA. Subsequently, results of CCK-8, EdU, Transwell, and aerobic glycolysis detection showed that CCDC183-AS1 plays an oncogene role in BC progression. Furthermore, an investigation of the downstream and upstream factors of CCDC183-AS1 identified a novel MITF/CCDC183-AS1/miR-4731-5p/TCF7L2 axis in BC progression, which might furnish novel insights for developing effective diagnostic and therapeutic strategies for BC.
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http://dx.doi.org/10.1155/2022/6785956DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357683PMC
July 2022
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