Publications by authors named "M Torabinejad"

197 Publications

Clinical and Histological Evaluation of Tissue Healing in Beveled or Perpendicular Vertical Releasing Incision.

J Endod 2021 Jul 9. Epub 2021 Jul 9.

Adjunct Professor of Endodonitcs, Loma Linda University School of Dentistry, 11092 Anderdon Street, Loma Linda, CA 92350.

Objectives: The aim of this investigation was to evaluate the clinical and histological differences in wound healing between beveled and perpendicular vertical releasing incisions in dogs.

Material And Methods: Four male mongrel dogs were used in this investigation. In each quadrant, a vertical releasing incision was made on either the mesial or the distal aspect of the cuspid teeth of each animal all on the same day. The sites were randomly selected to either receive a beveled incision or a perpendicular incision. A sulcular muco-periosteal flap was raised, reapproximated, and then sutured using 3-0 vicryl. The animals were evaluated daily for the first week, and then at different time intervals during this investigation. One animal was sacrificed at each time interval of 9, 14, 21 and 28 days after surgery. One animal was sacrificed at each time interval, and a bone block consisting of the cuspid teeth and their surrounding bone and soft tissues were harvested, formalin-fixed, and paraffin embedded. Samples were sectioned serially and stained with H&E. Specimens were evaluated using a microscope with magnification ranging from 10X to 400X by a histopathologist. Four indices were used for histological evaluation.

Results: Clinical results: At day 9 after surgery, a visible groove was seen in the marginal tissues of both perpendicular and beveled incisions. In the 14-day samples, a depression was still present at the perpendicular incision sites, but not in the beveled incision group, where the groove was not visualized at all. Histological results: At day 9, both of the histological sections showed less organized connective tissue and capillary networks with no significant difference in inflammation. At day 14, a depression was still present in the perpendicular incision but not in the beveled incision sites. In the 21 and 28 day samples, irregular capillary arrangements were seen in the connective tissue of the perpendicular incision groups with completely healed epithelium. There were no statistically significant differences noted histologically between the beveled and perpendicular incision groups noted at 9, 14, 21 or 28 days (P >0.05).

Conclusion: Based on these findings, it appears that clinical and histological healing of beveled or perpendicular releasing incisions are similar 28 days post-surgery.
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http://dx.doi.org/10.1016/j.joen.2021.07.005DOI Listing
July 2021

Henry John Van Hassel, DDS, MSD, PhD, 1933-2020: A Pioneer Research Icon.

J Endod 2021 05 27;47(5):684-689. Epub 2021 Feb 27.

Department of Endodontics, School of Dentistry, University of Washington, Seattle, Washington.

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http://dx.doi.org/10.1016/j.joen.2021.02.007DOI Listing
May 2021

Regeneration of Pulp-Dentin Complex in a Tooth with Symptomatic Irreversible Pulpitis and Open Apex Using Regenerative Endodontic Procedures.

J Endod 2021 Feb 10;47(2):247-252. Epub 2020 Oct 10.

Division of Endodontics, Department of Preventative and Restorative Dental Sciences, Loma Linda University School of Dentistry, Loma Linda, California.

Regenerative endodontic procedures are undertaken on permanent teeth with necrotic pulps and open apices in an attempt to resolve symptoms, to allow the continued development of the root(s), and to reestablish vitality. The available histologic analysis of these teeth has, in the majority of studies, shown that true regeneration of the pulp-dentin complex is not achieved. A recent investigation using an animal model outlined a procedure wherein pulpal amputation a few millimeters short of the apex followed by evoked bleeding allowed the complete regeneration of the normal pulp-dentin complex in immature vital teeth of ferrets. By implementation of this procedure, we report successful pulp regeneration outcome evidenced by continued root development and a positive response to pulp vitality tests in a maxillary central incisor with an open apex diagnosed with symptomatic irreversible pulpitis.
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http://dx.doi.org/10.1016/j.joen.2020.09.021DOI Listing
February 2021

Improving pulp revascularization outcomes with buccal fat autotransplantation.

J Tissue Eng Regen Med 2020 09 16;14(9):1227-1235. Epub 2020 Aug 16.

Department of Oral and Maxillofacial Pathology and Dental Implant Research Center, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.

Several techniques have been introduced to improve the pulp revascularization outcomes. The use of the tissue graft can create more practical tissue regeneration, provide vascular supply, and enhance tissue healing. The aim of the present study was to investigate the histologic and molecular outcomes of pulp revascularization with buccal fat autotransplantation. Fifty-six open apex roots from four dogs aged 4-6 months were randomly allocated to five groups of endodontic regeneration models: Group 1 (negative control, n = 4); Group 2 (control and without intervention, n = 4); Group 3 (blood clot, n = 16); Group 4 (buccal fat autotransplantation, n = 16); and Group 5 (blood clot plus buccal fat autotransplantation, n = 16). After 3 months, the extracted dog teeth were analyzed by histological and immunohistochemical techniques. Furthermore, real-time quantitative polymerase chain reactions were implemented to assess the gene expression profiles of dentin sialophosphoprotein (DSPP), dentin matrix protein (DMP), collagen I (COL1), and alkaline phosphatase (ALP) on regenerated tissue in the root canals. There were no significant differences in the severity of inflammation and necrosis between intervention groups. Immunohistochemical analysis showed significant differences among the study groups in expression level of extracellular glycoproteins such as fibronectin, laminin, and tenascin C. Group 5 showed an increase in the expression of DMP1 and COL1 genes. The expression of DSPP gene increased significantly in Group 4. The expression of ALP gene increased significantly in Group 3. Using this procedure may open new fields of research for regenerative endodontic procedure in which tissue autotransplant, particularly adipose tissue, may improve the outcomes of pulp revascularization.
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http://dx.doi.org/10.1002/term.3094DOI Listing
September 2020

Regenerative Endodontic Treatment in Immature Noninfected Ferret Teeth Using Blood Clot or SynOss Putty as Scaffolds.

J Endod 2020 Feb 16;46(2):209-215. Epub 2019 Dec 16.

Advanced Specialty Education Programs in Endodontics, School of Dentistry, Loma Linda University, Loma Linda, California.

Introduction: SynOss Putty (Collagen Matrix, Oakland, NJ) has shown the formation of mineralized tissues when used as a scaffold in regenerative endodontic treatment (RET) in immature human teeth. The aim of this study was to compare the outcome of RET in immature ferret teeth using 2 scaffolds: a blood clot and SynOss Putty.

Methods: Thirty-two immature canine teeth in 8 ferrets (95-105 days old) were divided into 4 groups: group 1, no treatment (positive control, n = 8); group 2, full pulpectomy with no further treatment (negative control, n = 8); group 3, revascularization using a blood clot (n = 8); and group 4, revascularization using a SynOss Putty scaffold (n = 8). After 3 months, the animals were euthanized, and the newly formed tissues were examined histologically. The data were statistically analyzed using chi-square and Fisher exact tests.

Results: Normal pulps were found in group 1. No pulp tissue was found in teeth in group 2. In group 3, the pulp tissue and the odontoblastic layer were absent, and the root canal spaces were filled with a hard tissue characterized as bonelike and cementumlike tissues. All teeth except 1 in group 4 showed no hard tissue formation and intracanal/periapical inflammation. SynOss Putty was significantly associated with a lack of tissue formation and intracanal/periapical inflammation (P < .05).

Conclusions: Intracanal hard tissue formation was observed in immature teeth after RET using a blood clot. No tissue regeneration was found in the majority of samples using SynOss Putty as a scaffold.
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http://dx.doi.org/10.1016/j.joen.2019.10.029DOI Listing
February 2020
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