Publications by authors named "M Rameshpathy"

2 Publications

  • Page 1 of 1

Emergence of plasmid-borne gene in : first report from India.

Access Microbiol 2019 15;1(3):e000024. Epub 2019 May 15.

Antibiotic Resistance and Phage Therapy Laboratory, Department of Biomedical Sciences, School of Bioscience and Technology, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India.

Wastewater has become a potential habitat for multi-drug-resistant bacteria. The present study aims to screen for the presence of carbapenem-resistant bacteria in sewage water samples collected from hospital and non-hospital sources. From a total of 19 sewage water samples collected, 100 carbapenem-resistant non-lactose-fermenting Gram-negative bacteria (CR-NF-GNB) were isolated using MacConkey agar cultured with 8 mg l of meropenem. On screening for beta-lactamase resistance genes ( , , , and ), one isolate, , was found to carry the plasmid-borne gene. To the best of our knowledge, we provide the first report of the rare and emerging opportunistic pathogen encoding the OXA-181 gene in its plasmid.
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http://dx.doi.org/10.1099/acmi.0.000024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471779PMC
May 2019

Human sperm DNA damage inhibition and antioxidant activity of T. arjuna bark: an in vitro study.

3 Biotech 2017 Jul 29;7(3):188. Epub 2017 Jun 29.

Gene Cloning and Technology, SBST, VIT University, Vellore-14, India.

Complimentary or natural antioxidant type of alternative medicine is developed worldwide to treat male infertility. The aim of this study is to the extraction of T. arjuna bark and activity against human sperm DNA damage in asthenoteratospermic smoker's subjects-an in vitro study. All preliminary and antioxidant assays (DPPH, HO, and total antioxidant, reducing power activity) were done. T. arjuna bark metal analysis was done with AAS. On the other hand, patients were asked to fill a direct questionnaire about smoking history; 25 infertile smokers were identified as asthenoteratospermic; 34 fertile non-smokers (control) were assessed for semen parameters by CASA, seminal plasma Zinc analysis by AAS, DNA fragmentation by colorimetric method and semen genomic DNA damage inhibition by modified non-enzymatic salting out extraction method. Most of the antioxidants are highly present in the aqueous extract; meanwhile, the major content in this extract is zinc 16 µg/g (Ca = 0.5 µg/g; Se = 2.2 µg/g and Mg = 1.6 µg/g) along with FT-IR peaks which also confirmed the metal presence. The semen parameters in smokers that were noticed are low sperm count and morphological changes. Meanwhile, in the seminal plasma of smokers, zinc and DNA fragmentation results were positively correlated with sperm morphology (p < 0.001). Repaired DNA bands were noticed in the in vitro study of aqueous T. arjuna bark, in smokers' semen. T. arjuna bark will act as cryo protector as well as great zinc supplementary to maintain sperm motility and morphology in smokers.
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http://dx.doi.org/10.1007/s13205-017-0853-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491448PMC
July 2017