Publications by authors named "M Priyanka"

21 Publications

Columella necrosis in a child secondary to nasal continuous positive airway pressure during neonatal period.

Med J Malaysia 2021 Sep;76(5):771-773

University Malaya Medical Centre, Department of Otorhinolaryngology, Malaysia.

The advent of continuous positive airway pressure ventilation as a mode of treatment for respiratory distress syndrome for premature infants has increased the risk of nasal injuries such as pressure necrosis. We describe a case of a 24-week infant who received CPAP ventilation as a mode of ventilatory support for respiratory distress syndrome and the complication of pressure necrosis of the columella. There are many factors that predispose an infant receiving CPAP ventilation to nasal injury. Many strategies can be employed to reduce the incidence of nasal injuries such as the use of nasal barrier dressings, the use of nasal high flow oxygen (nHF) cannula instead of CPAP ventilation, and the use of nasal masks instead of nasal prongs for CPAP ventilation delivery. The treatment of pressure necrosis can be either medical or surgical. The use of ointments or growth sprays can be used in cases of skin breakdown. Surgical reconstruction can be offered in cases of nasal deformity.
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September 2021

Early-stage culprit in protein misfolding diseases investigated using electrochemical parameters: New insights over peptide-membrane interactions.

Biomed Pharmacother 2021 Oct 28;142:111964. Epub 2021 Jul 28.

Department of Applied Chemistry, School of Applied Natural Science, Adama Science and Technology University, P O Box 1888, Adama, Ethiopia. Electronic address:

The dysfunctioning of β-cells caused by the unspecific misfolding of the human islet amyloid polypeptide (hIAPP) at the membrane results in type 2 diabetes mellitus. Here, we report for the first time, the early-stage interaction of hIAPP oligomers on the DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) lipid membrane using electrochemical parameters. Electrochemical techniques are better than other techniques to detect hIAPP at significantly lower concentrations. The surface level interactions between the peptide (hIAPP) and lipid membrane (DMPC) were investigated using atomic force microscopy (AFM), confocal microscopy (CM) and electrochemical techniques such as Tafel polarization, cyclic voltammetry (CV), differential pulse voltammetry (DPV), linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS). Inserting IAPP into the fluid domains results in breaking the lipid-to-lipid interaction, leading to restriction of membrane mobility. The S values of the liposome and IAPP co-solubilized liposome indicates the cooperative insertion of IAPP. Further, a new method of immobilizing a membrane to the gold surface has been employed, resulting in an electrical contact with the buffer, preventing the direct utilization of a steady-state voltage across the bilayer. The electrochemical studies revealed that the charge transfer resistance decreased for 3-mercaptopropanoic acid modified gold (MPA-Au) electrode coated with the liposome and after the addition of IAPP, followed by an increase in the capacitance. The present study has opened up new dimensions to the understanding of peptide-membrane interactions and shows different experimental approaches for the future researchers in this domain.
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October 2021

Prevalence, service use and clinical correlates of hallucinations and delusions in an out-patient population from India.

J Ment Health 2021 Jun 21:1-9. Epub 2021 Jun 21.

The Banyan Academy of Leadership in Mental Health (BALM), Kanchipuram, India.

Background: Despite the persistent public health problem of positive psychotic symptoms, understanding of symptom specific prevalence rates, clinical correlates and service utilisation are sparse.

Aims: The current study aimed to establish prevalence, clinical and service utilisation correlates of hallucinations and delusions in people accessing outpatient clinics in Tamil Nadu, India.

Methods: Secondary patient data from outpatient clinics, over a 12-month period, in 2016, was used for analysis ( = 917). Based on the presence of positive psychotic symptoms (PPSx), the sample was divided into four groups for analysis- hallucinations-only (H), delusions-only (D), both hallucinations and delusions (HD) and neither PPSx (N-PPSx).

Results: Findings indicate that the most prevalent PPSx were hallucinations (10.7%) however, barriers to service utilisation and clinical correlates were associated predominantly with the D and the HD group; as was severe work impairment. Yet, this group was most likely to remain with psychiatric services. Lastly, diagnostic challenges were apparent within the sample.

Conclusions: The study revealed that despite more barriers to service utilisation, persons with PPSx remain in contact with services. Yet prognosis remains only moderate at best, indicating other mediating and underlying factors impeding recovery may be interplaying and, therefore, a need for enhanced biopsychosocial approaches.
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June 2021

Stereomicroscope as an aid in grossing and histopathological diagnosis: A prospective study.

J Oral Maxillofac Pathol 2020 Sep-Dec;24(3):459-465. Epub 2021 Jan 9.

Department of Oral Pathology, Government Dental College and Hospital, Hyderabad, Telangana, India.

Background: Routinely, naked eye and a magnified glass are used to observe the specimens during grossing procedure. Stereomicroscope is a significant adjuvant to examine a variety of specimens and it enables us to examine the surface details of the specimen efficiently.

Aims And Objectives: To assess the role of stereomicroscope in macroscopic examination and its effectiveness in the study of surface details of the specimen, thereby evaluating its role as an effective tool in grossing and histopathological diagnosis.

Materials And Methods: The study sample consisted of routine specimens received by the Department of Oral and Maxillofacial Pathology, Government Dental College and Hospital, Hyderabad, between July 15, 2018, and September 15, 2018. All the specimens were photographed under direct daylight ad later examined under stereomicroscope. The images were compared and grossing was carried out. The grossed specimen were processed using standard procedure and histopathological diagnosis was made.

Results: Compared to naked eye, the stereomicroscopic observation of specimens showed better details which helped in proper orientation of the specimen.

Conclusion: Stereomicroscope aids as an adjuvant for proper orientation of the specimen which helps in arriving at an accurate final diagnosis.
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January 2021

Generation of single stranded DNA with selective affinity to bovine spermatozoa.

Asian-Australas J Anim Sci 2020 Aug 21. Epub 2020 Aug 21.

Director, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India.

Objective: To generate single stranded DNA oligonucleotides with selective affinity to bovine spermatozoa, assess its binding potential and explore its potential utility in trapping spermatozoa from suspensions.

Methods: A combinatorial library of 94 mer long oligonucleotide was used for Systematic Evolution of Ligands by Exponential Enrichment [SELEX] with bovine spermatozoa. The amplicons from sixth and seventh rounds of SELEX were sequenced, and the reads were clustered employing Cluster Database at High Identity with Tolerance [CD-HIT] and FASTAptamer. The enriched nucleotides were predicted for secondary structures by Mfold, motifs by Multiple Em for Motif Elicitation (MEME) and 5' labelled with biotin/ 6-FAM to determine the binding potential and binding pattern.

Results: We generated 14.1 and 17.7 million reads from sixth and seventh rounds of SELEX respectively to bovine spermatozoa. The CD-HIT clustered 78098 and 21196 reads in the top ten clusters and FASTAptamer identified 2195 and 4405 unique sequences in the top three clusters from the sixth and seventh rounds, respectively. The identified oligonucleotides formed secondary structures with delta G values between -1.17 to -26.18 kcal/ mol indicating varied stability. Confocal imaging with the oligonucleotides from the seventh round revealed different patterns of binding to bovine spermatozoa [fluorescence of the whole head, spot of fluorescence in head and mid- piece and tail]. Use of a 5'-biotin tagged oligonucleotide from the sixth round at 100 pmol with 4 x 106 spermatozoa could trap almost 80% from the suspension.

Conclusion: The binding patterns and ability of the identified oligonucleotides confirms successful optimization of the SELEX process and generation of aptamers to bovine spermatozoa. These oligonucleotides provide a quick approach for selective capture of spermatozoa from complex samples. Future SELEX rounds with X- or Y- enriched sperm suspension will be used to generate oligonucleotides that bind to spermatozoa of a specific sex type.
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August 2020