Publications by authors named "M Lozano Ros"

430 Publications

Time-sequenced transcriptomes of developing distal mouse limb buds: A comparative tissue layer analysis.

Dev Dyn 2021 Jul 12. Epub 2021 Jul 12.

Instituto de Biomedicina y Biotecnología de Cantabria, IBBTEC (CSIC-University of Cantabria-SODERCAN), Santander, Spain.

Background: The development of the amniote limb has been an important model system to study patterning mechanisms and morphogenesis. For proper growth and patterning, it requires the interaction between the distal sub-apical mesenchyme and the apical ectodermal ridge (AER) that involve the separate implementation of coordinated and tissue-specific genetic programs.

Results: Here, we produce and analyze the transcriptomes of both distal limb mesenchymal progenitors and the overlying ectodermal cells, following time-coursed dissections that cover from limb bud initiation to fully patterned limbs. The comparison of transcriptomes within each layer as well as between layers over time, allowed the identification of specific transcriptional signatures for each of the developmental stages. Special attention was given to the identification of genes whose transcription dynamics suggest a previously unnoticed role in the context of limb development and also to signaling pathways enriched between layers.

Conclusion: We interpret the transcriptomic data in light of the known development pattern and we conclude that a major transcriptional transition occurs in distal limb buds between E9.5 and E10.5, coincident with the switch from an early phase continuation of the signature of trunk progenitors, related to the initial proximo distal specification, to a late intrinsic phase of development.
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http://dx.doi.org/10.1002/dvdy.394DOI Listing
July 2021

Environmental stress responses in sympatric congeneric crustaceans: Explaining and predicting the context-dependencies of invader impacts.

Mar Pollut Bull 2021 Jun 17;170:112621. Epub 2021 Jun 17.

Laboratoire Environnement de Petit Saut, Hydreco-Guyane, BP 823, 97310 Kourou, French Guiana; Laboratorio de Fisiología y Genética Marina (FIGEMA), Departamento de Acuicultura, Facultad de Ciencias del Mar, Universidad Católica del Norte, Coquimbo, Chile.

The role of ecophysiology in mediating marine biological pollution is poorly known. Here we explore how physiological plasticity to environmental stress can explain and predict the context-dependencies of invasive species impacts. We use the case of two sympatric skeleton shrimps, the invader Caprella scaura and its congener C. equilibra, which is currently replaced by the former on the South European coast. We compare their physiological responses to hyposalinity stress under suboptimal low and high temperature, while inferring on hypoxia tolerance. We use an energy-redox approach, analyzing mortality rate, the energetic balance and the consequent effects on the oxidative homeostasis. We found that decreased seawater salinity and/or oxygen levels can weaken biotic resistance, especially in females of C. equilibra, leading to periods of heightened vulnerability to invasion. Our approach provides mechanistic insights towards understanding the factors promoting invader impacts, highlighting the potential of ecophysiology for improving invasive species management.
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http://dx.doi.org/10.1016/j.marpolbul.2021.112621DOI Listing
June 2021

Keys to discern the Phoenician, Punic and Roman mining in a typical coastal environment through the multivariate study of trace element distribution.

Sci Total Environ 2021 May 26;790:147986. Epub 2021 May 26.

University of Natural Resources and Applied Life Sciences, Peter Johan Str. 82, 1190 Viena, Austria. Electronic address:

Trace element concentrations in the Cartagena Bay coastal record reveal a contribution of natural processes. However, the influence of anthropogenic factors predominates in the last three millennia, particularly aerosol deposition linked to mining and industrial activities in the area. The coastal record of Cartagena can be considered a preserved environment, suitable to search for regional human activity fingerprinting, specifically that related to the deposition of heavy metals such as Pb and Cu. A multivariate statistical analysis was carried out to clarify the geochemical behaviour of trace and major elements. Our study design represents a novel approach to assign natural contributions, such as eolian and riverine input, to coastal deposits, and organic matter preservation under anoxic environments. Therefore, synergies obtained by the simultaneous study of multivariate statistics and enrichment factors allow robust conclusions about palaeoenvironmental evolution and human activities. Anthropogenic influence suggested that Pb mining and metallurgy began during the Chalcolithic period, with considerable inputs of Pb and Cu to atmospheric pollution during Phoenician, Punic and Roman times.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147986DOI Listing
May 2021

Impact of COVID-19 on nutritional status during the first wave of the pandemic.

Clin Nutr 2021 May 8. Epub 2021 May 8.

Endocrinology and Nutrition Service, Hospital Universitari Germans Trias i Pujol, Badalona, Spain; Autonomous University of Barcelona, Barcelona, Spain. Electronic address:

Background & Aims: Patients affected by COVID-19 may develop disease related malnutrition (DRM) due to the catabolic situation, symptoms that interfere with intake and prolonged hospital stay. This study aims to know the percentage of patients admitted for COVID-19 who required artificial nutrition (AN), their clinical characteristics, as well as the prevalence of DRM and the risk of sarcopenia at hospital discharge and after 6 months.

Material And Methods: Observational, prospective study, with successive inclusion of adult patients admitted for COVID-19 in whom institutional nutritional support (NS) care protocol was applied. Those who received AN underwent a nutritional screening by Short Nutritional Assessment Questionnaire (SNAQ) and an assessment by Subjective Global Assessment (SGA) at hospital discharge, as well as a screening for sarcopenia (SARC-F test) and SNAQ re-test 15 days and 6 months after by a phone call. Symptoms related to food intake, anthropometric and analytical data were also collected.

Results: We evaluated 936 patients with a mean age of 63.7 ± 15.3 years; predominantly male (59.7%), overweight 41%, obesity 40.4%; hypertension 52.9%; diabetes mellitus 26.6% and cancer 10.4%. The stay hospital length was 17.3 ± 13.8 days and 13.6% patients died during hospitalization. The modality of nutritional support was: 86.1% dietary adaptation + oral nutritional supplements (ONS); 12.4% enteral nutrition (EN) by nasogastric (NG) tube; 0.9% parenteral nutrition (PN) and 0.6% EN plus PN. Focusing on patients who received AN, follow-up post discharge was possible in 62 out of 87 who survived. Of these, at the time of hospital discharge, 96.7% presented nutritional risk by SNAQ and 100% malnutrition by SGA (20% B; 80% C). During admission, 82.3% presented intense anorexia and the mean weight loss was 10.9 ± 6 Kg (p < 0.001). Fifteen days after being discharged, 12.9% still had anorexia, while hyperphagia appeared in 85.5% of the patients and risk of sarcopenia by SARC-F was present in 87.1% of them. Six months after discharge, 6.8% still had anorexia and 3.4% hyperphagia, with a global weight gain of 4.03 ± 6.2 Kg (p=<0.0001). Risk of malnutrition was present in only 1.7% of the patients, although risk of sarcopenia persisted in 49.2%.

Conclusion: All patients admitted by COVID-19 for whom EN or PN were indicated following an institutional protocol still presented malnutrition at hospital discharge, and almost all showed risk of sarcopenia, that persisted in almost half of them at 6 months. These findings suggest that nutritional and functional problems persist in these patients after discharge, indicating that they require prolonged nutritional support and monitoring.
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http://dx.doi.org/10.1016/j.clnu.2021.05.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106234PMC
May 2021

Home physiotherapists assisting follow-up treatment in cystic fibrosis: a multicenter observational study.

Monaldi Arch Chest Dis 2021 Apr 15;91(2). Epub 2021 Apr 15.

Cystic Fibrosis Centre, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan; 3Department of Clinical Sciences and Community Health, Laboratory of Medical Statistics, Epidemiology and Biometry G. A. Maccacaro, University of Milan .

Inhaled therapies are relatively simple and easy to be managed however ineffective use of aerosols when self-administered may occur. We described variation of the number of clinic visits, lung function and number of antibiotic courses performed over 12 months in participants with cystic fibrosis (CF), when supervised or not by physiotherapists (PTs) at home. Participants in 8 Italian CF centers with a prescription of dry-powder antibiotic choose whether to be supervised at home (PT-FU) or not (non-PT-FU), in adjunct to routine clinic visits. PTs assisted participants with their inhaled therapies regimen and reviewed the airway clearance program in use.  Mixed-effect regression models were fitted to evaluate the variation of selected endpoints over time. A total of 163 participants were included.  Lung function declined over time in both groups, at higher extent in the non-PT-FU group at 6 months (-1.8, 95%CI: -4.4 to 0.7 % predicted), without reaching statistical significance, whereas in the PT-FU group only, nearly one visit less was recorded (p=0.027). Regardless the type of supervision adopted, the number of antibiotic courses did not change compared to the previous year. We counted 19/90 (21.1%) drop-out in the PT-FU, double compared to the group followed up at the clinics (p=0.065). Participants under a course of an inhaled antibiotic therapy showed a 1-year decline in lung function, whereas only the group receiving home supervision counted nearly one visit less at the CF center, whose clinical relevance should be further discussed.
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http://dx.doi.org/10.4081/monaldi.2021.1619DOI Listing
April 2021