Publications by authors named "M L Zlochevskiĭ"

10 Publications

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[Cloning of the RIB7 gene encoding the riboflavin synthase of the yeast Pichia guilliermondii].

Genetika 1993 Jun;29(6):922-7

The RIB7 gene encoding the enzyme of the final stage of riboflavin biosynthesis in Pichia guilliermondii--riboflavin synthase was cloned on the pFL38 shuttle vector as the Sau3A fragment of the chromosomal DNA of about 4 kb. The HindIII fragment of 1.4 kb was subcloned from the hybrid plasmid pFR7 obtained onto the pUC18 plasmid. The plasmid pR7 thus constructed transform Escherichia coli ribB-45 mutant cells with a blocked riboflavin synthase approximately at the same frequency as pFR7. High riboflavin synthase activity was discovered in the E. coli transformants carrying pR7 but not pFR7. Using both plasmids we also complemented rib17 mutant of P. guilliermondii.
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June 1993

[Cloning and inactivation of a chromosomal copy of the imidazole glycerophosphate dehydratase (HIS) gene from Hansenula polymorpha].

Mol Gen Mikrobiol Virusol 1991 Jul(7):25-8

A gene for imidazole glycerophosphate dehydratase (HIS) has been selected from the library of Hansenula polymorpha genes by complementation of Escherichia coli hisB mutations or his3 mutation of Saccharomyces cerevisiae. Inactivation of the gene in Hansenula polymorpha strain 48V, as well as Leu(-)-Leu+ conversion of phenotype and arising of antibiotic G418 resistance resulted from transformation of the strain by the linear DNA molecules of the cloned HIS-gene with the in vitro inserted LEU2 gene from Saccharomices cerevisiae and KmR gene. The Southern hybridization analysis of the Leuf+His- G418R clones representing 1-2% of transformants population together with the DNA analysis of the monospore clones from the hybrid Leu+His-G418R transformant tetrad and tester strain have revealed the locus specific nature of the clones.
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July 1991

[Evaluation of the stability of algorithms in data processing by computerized diagnostic cardiocomplex ECG C3T-01].

Authors:
M S Zlochevskiĭ

Med Tekh 1990 Nov-Dec(6):12-6

Evidence is provided for the model of choosing cardiosignal depiction to estimate one of the most important parameters of an outfit operation - stability of the results of electrocardiosignal (ECS) processing. It should be mentioned that despite the fact that the given parameter is not so far generally acknowledged, it reflects accurately enough the operation "reliability" of the automated system. The author bases theoretically the choice of the adaptive piecewise model of ECS. Describes the results of testing real ECS estimated according to 3 different processing algorithms bearing in mind the protection of the latter ones from additive interference of different types. Analogous studies were also carried out with artificial ECS. Analyzing the research findings the author arrives at the conclusion that the up-to-date automated systems are to be equipped with appliances enhancing the computation stability of algorithms of signal processing.
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April 1991

[Cloning of the RIB1 gene coding for the enzyme of the first stage of flavinogenesis in the yeast Pichia guilliermondi, GTP cyclohydrolase, in Escherichia coli cells].

Genetika 1990 Apr;26(4):614-20

The RIB1 gene encoding the enzyme of the first stage of the yeast Pichia guillermondii-GTP-cyclohydrolase- was cloned on pFL38 shuttle vector as the Sau3A fragment of chromosomal DNA of about 9 kb. EcoRI fragment of 4 kb with RIB1 gene was subcloned from the pFRI hybrid plasmid obtained into the pUC18 plasmid and then shortened to give 2.9 kb via deletion in SalGI site. The plasmid constructed was designated pR1. Activity of GTP-cyclohydrolase was 80-100-fold higher in extracts of transformants than in the prototroph strain, which evidence of effective expression of the yeast gene within recombinant plasmids in the cells of this species of bacteria. The enzyme isolated from transformants has molecular mass 179 kDa, is inhibited by PAD and adenyl-nucleotides, which is characteristic of GTP-cyclohydrolase of P. guilliermondii but not of Escherichia coli.
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April 1990

[A complex unit EKGS3T-01 for ECG recording and processing with preserving the syndromal findings].

Med Tekh 1990 Mar-Apr(2):38-41

Functional and technical parameters of the microprocessor diagnostic complex are being considered herein. It allows one to combine the automatic ECG data analysis and the possibility of the active participation in the processing of the cardiological information.
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September 1990

[Expression of human leukocyte interferon type A in Saccharomyces cerevisiae under the control of regulatory elements of the yeast gene URA3].

Mol Gen Mikrobiol Virusol 1987 May(5):26-32

Expression of a chromosomal gene for human leucocyte interferon A was obtained due to interaction of 5'-nontranslating region of a cloned interferon gene with the regulatory sequences from UNA3 yeast gene. The sequence of 5'-nontranslating region from interferon gene essential for its expression in yeast is localized within 130 b p. from the initiating codon. Due to increasing of plasmid stability and copy number a 60-fold increase. in interferon yield was obtained in yeast transformants reaching the level of 6 X 10(7) u X 1(-1). The data are presented supposing the existence of functional polycistronic mRNA in yeast.
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May 1987

[Cloning and the determination of the nucleotide sequences in 2 genes of human leukocyte interferons].

Antibiot Med Biotekhnol 1986 Aug;31(8):592-6

Four different recombinant phages carrying interferon sequences were identified in the human gene bank on vector Charon 4A. A fragment of interferon A gene cloned previously through cDNA was used as a hybridization probe for analysis of the bank. The nucleotide sequence of DNA encoding the interferon genes was determined for 2 recombinant phages. One of these genes corresponded to the interferon A gene but one amino acid substitution (His 34-Arg). The second gene (I1) belonged to the family of the interferon C genes and differed from all the genes described earlier.
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August 1986

[Complex of single-stranded phage DNA and protein--a product of bacteriophage fl gene 3. Distribution of gene 3 protein in the DNA molecule].

Mol Biol (Mosk) 1978 May-Jun;12(3):505-14

Upon a 50% isopropanol treatment of phage fl in a 1 M NaCl solution protein A (gene 3 product)--DNA complex is precipitated while protein B (gene 8 product) was still solubilized. After such a treatment the DNA--protein complex containing 10--40% of protein A and less than 0.0025% of protein B was obtained. Evidence was obtained that there was no non-specific rearrangement of protein A during the isolation procedure. The complex was treated with endonuclease R.HAC III, followed by electrophoresis of the resulted fragments and estimation of the [14C] protein A (labeled with [14C]histidine) throughout the gel. The maximal radioactivity coincided with the DNA bands, being proportional to the DNA content in the respective bands. The data obtained indicate that protein A is iniformly arranged along the DNA molecule.
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August 1978