Publications by authors named "M L Avinash Tejasvi"

8 Publications

Individuals Age Determination from Human Dental Pulp Through DNA Analysis by PCR.

Glob Med Genet 2021 Jun 15;8(2):57-61. Epub 2021 Feb 15.

Department of Genetics, Central Research Laboratory, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India.

 Age estimation in forensic odontology is having a great importance in recent times because of the request by court or other government authorities so that immigrants whose real age is unknown should not suffer unfair disadvantages because of their supposed age, and so that all legal procedures to which an individual's age is relevant can be properly followed.  The present study was planned to be conducted on pulp tissue and dental hard tissues derived from individuals for DNA isolation and age determination .  The present study was an experimental single-blinded study consisting of 30 extracted teeth categorized into three groups as follows: Group A: 10 to 20 years, Group B: 21 to 30 years, Group C: 31 to 40 years. DNA was isolated from the pulp of each tooth and quantitative polymerase chain reaction (qPCR) for calculating telomere length was performed.  With increase in age, the length of telomere gets shortened which will be helpful in analyzing the age of the person when morphological and biological remnants are not available except the tooth.  The present study found that estimation of human age based on the relative TL measured by the real-time quantitative PCR may be a useful method for age prediction, especially when there is no morphologic information in the biological sample. This is the first study to accesses the age of a person by telomere length using dental pulp.
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http://dx.doi.org/10.1055/s-0041-1723084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110361PMC
June 2021

Genetic Disorders of Bone or Osteodystrophies of Jaws-A Review.

Glob Med Genet 2021 Jun 15;8(2):41-50. Epub 2021 Mar 15.

Department of Oral Pathology, Tirumala Institute of Dental Sciences, Nizamabad, Telangana, India.

Bone is a specialized form of connective tissue, which is mineralized and made up of approximately 28% type I collagen and 5% noncollagenous matrix proteins. The properties of bone are very remarkable, because it is a dynamic tissue, undergoing constant renewal in response to mechanical, nutritional, and hormonal influences. In 1978, "The International Nomenclature of Constitutional Diseases of Bone" divided bone disorders into two broad groups: osteochondrodysplasias and dysostoses. The osteochondrodysplasia group is further subdivided into two categories: dysplasias (abnormalities of bone and/or cartilage growth) and osteodystrophies (abnormalities of bone and/or cartilage texture). The dysplasias form the largest group of bone disorders, hence the loose term "skeletal dysplasia" that is often incorrectly used when referring to a condition that is in reality an osteodystrophy or dysostosis. The word "dystrophy" implies any condition of abnormal development. "Osteodystrophies," as their name implies, are disturbances in the growth of bone. It is also known as osteodystrophia. It includes bone diseases that are neither inflammatory nor neoplastic but may be genetic, metabolic, or of unknown origin. Recent studies have shown that bone influences the activity of other organs, and the bone is also influenced by other organs and systems of the body, providing new insights and evidencing the complexity and dynamic nature of bone tissue. The 1,25-dihydroxyvitamin D3, or simply vitamin D, in association with other hormones and minerals, is responsible for mediating the intestinal absorption of calcium, which influences plasma calcium levels and bone metabolism. Diagnosis of the specific osteodystrophy type is a rather complex process and various biochemical markers and radiographic findings are used, so as to facilitate this condition. For diagnosis, we must consider the possibility of lesions related to bone metabolism altered by chronic renal failure (CRI), such as the different types of osteodystrophies, and differentiate from other possible neoplastic and/or inflammatory pathologies. It is important that the dentist must be aware of patients medical history, suffering from any systemic diseases, and identify the interference of the drugs and treatments to control them, so that we can able to perform the correct diagnosis and propose the most adequate treatment and outcomes of the individuals with bone lesions.
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http://dx.doi.org/10.1055/s-0041-1724105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110367PMC
June 2021

Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method.

Glob Med Genet 2021 Mar 23;8(1):19-23. Epub 2021 Feb 23.

Department of Oral Medicine and Radiology, HKDET Dental College and Hospital Humnabad, Karnataka, India.

 While DNA profiling has become the principal technique for individualization of biological evidences, ABO blood grouping is still a useful test method in the initial stages of crime investigation. Objectives of the study were blood group determination using slide agglutination method, blood group determination from saliva using absorption inhibition method, and comparison of slide agglutination method with that of absorption inhibition method from saliva sample.  A total of 60 subjects were taken randomly with their age ranging from 20 to 60 years. Sixty subjects were divided in to two groups, study group and control group. 5 to 10 mL of unstimulated saliva was collected from 60 patients and Wieners agglutination test was performed to detect the secretor status of blood using absorption inhibition method and compared with that of slide agglutination method  Out of 60 subjects, 52 subjects showed secretors of antigen in saliva with percentage value of 86.66% and eight subjects were nonsecretors (13.33%). Slightly higher percentage of secretor status was seen in males 84.6 and 88.2% in females.  Evaluation of secretor status of blood group antigen from saliva using absorption inhibition method can be useful method in identification of medicolegal cases.
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http://dx.doi.org/10.1055/s-0041-1723083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7964258PMC
March 2021

Impact of ERCC2 Gene Polymorphisms on OSCC Susceptibility and Clinical Characteristics.

Glob Med Genet 2020 Dec 18;7(4):121-127. Epub 2021 Feb 18.

Department of Prosthodontics, Kamineni Institute of Dental Sciences Narketpally, Telangana, India.

 DNA repair systems play an important role in maintaining the integrity of the human genome. Deficiency in the repair capacity due to either mutations or inherited polymorphisms in DNA repair genes may contribute to variations in the DNA repair capacity and subsequently susceptibility to cancer.  This study aimed to investigate the association between Excision repair cross-complementation groups 2 (ERCC2) single nucleotide polymorphisms (SNPs rs1799793 and rs13181) and the response to platinum-based chemotherapy among patients with oral squamous cell carcinoma (OSCC).  Polymerase chain reaction-based restriction fragment length polymorphism analysis was used to determine the polymorphism from a total of 150 OSCC patients and 150 normal tissues of same patients were collected as controls for this study.  ERCC2 GA (Asp312Asn) AC (Lys751Gln) genotypes were significantly associated (  0.0001 and  = 0.0004, respectively) with OSCC patients, when compared with the controls. These findings suggest that potentially functional SNPs in may contribute to OSCC risk. This study highlights the genetic variant that might play a role in mediating susceptibility to OSCC in this population. An understanding of DNA repair gene polymorphisms might not only enable risk assessment, but also response to therapy, which target the DNA repair pathway.
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http://dx.doi.org/10.1055/s-0041-1722952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938941PMC
December 2020

Comparison of Gender Determination from Dental Pulp and Dentin after Exposure to Various Environmental Conditions: A Polymerase Chain Reaction-based SRY Gene Study.

Contemp Clin Dent 2019 Apr-Jun;10(2):256-262

Private Practioner, Sai Venkateswara Dental Clinic, Ramantapur, Hyderabad, Telangana, India.

Purpose: Sex determination becomes the first priority by a forensic investigator in the process of identification of bodies mutilated beyond recognition due to mass disasters. Sex-determining region Y (SRY) gene is used as a sex-typing marker as the results are said to be 100% accurate. Therefore, an experimental study was conducted to rule out the possibility of the presence of SRY gene in tooth pulp and dentin after being exposed to various environments.

Materials And Methods: Twenty extracted teeth of known gender were taken, of which ten were male and ten were female. The tooth samples were subjected to various environmental conditions for 60 days.

Results: Among the total twenty samples, DNA isolation was done from all the samples of pulp and dentin. Gender determination using SRY gene was correct in all the samples of the pulp, and no interpretation of SRY was possible from the dentin though the DNA was isolated.

Conclusion: The present study has found DNA retrieval can be done from human teeth irrespective of the storage time and environmental conditions for forensic caseworks and SRY gene to be a reliable biomarker for sex determination from the pulp tissue of teeth.
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http://dx.doi.org/10.4103/ccd.ccd_472_18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7145251PMC
April 2020