Publications by authors named "M Anitha"

55 Publications

The aryl hydrocarbon receptor activates ceramide biosynthesis in mice contributing to hepatic lipogenesis.

Toxicology 2021 06 9;458:152831. Epub 2021 Jun 9.

Center for Molecular Toxicology and Carcinogenesis, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, 16802, USA. Electronic address:

Aryl hydrocarbon receptor (AHR) activation via 2,3,7,8-tetrachlorodibenzofuran (TCDF) induces the accumulation of hepatic lipids. Here we report that AHR activation by TCDF (24  μg/kg body weight given orally for five days) induced significant elevation of hepatic lipids including ceramides in mice, was associated with increased expression of key ceramide biosynthetic genes, and increased activity of their respective enzymes. Results from chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assay (EMSA) and cell-based reporter luciferase assays indicated that AHR directly activated the serine palmitoyltransferase long chain base subunit 2 (Sptlc2, encodes serine palmitoyltransferase 2 (SPT2)) gene whose product catalyzes the initial rate-limiting step in de novo sphingolipid biosynthesis. Hepatic ceramide accumulation was further confirmed by mass spectrometry-based lipidomics. Taken together, our results revealed that AHR activation results in the up-regulation of Sptlc2, leading to ceramide accumulation, thus promoting lipogenesis, which can induce hepatic lipid accumulation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tox.2021.152831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327397PMC
June 2021

Comparison of Jet Injection Technique with Conventional Injection Technique in Patients Undergoing Endodontic Therapy.

Contemp Clin Dent 2021 Jan-Mar;12(1):28-31. Epub 2021 Mar 20.

Department of Oral Medicine and Radiology, Ultra's Best Dental Science College, Madurai, Tamil Nadu, India.

Background: In order to reduce the fear and anxiety of injection during root canal procedures, Madajet injection technique was substituted for the first time among the adult patients to obtain its efficacy and to overcome the painful procedure during the conventional syringe technique.

Aim: The aim of this study is to compare the clinical efficacy and level of patient acceptance of jet injections with conventional syringe technique in patients with symptomatic pulpitis.

Methodology: Seventy patients were randomly divided into needleless pressure injection technique using Madajet XL and conventional syringe technique. The onset of anesthesia was evaluated using the electric pulp tester, and the pain was assessed using the Visual Analog Scale scoring criteria.

Statistical Analysis: The obtained data were tabulated and subjected to the statistical analysis using the Chi-square test.

Results: Needleless pressure injection technique (Madajet XL) proves to be effective in patients with symptomatic irreversible pulpitis during endodontic procedure.

Conclusion: It can be concluded that the needle-less pressure injection technique (Madajet XL) promises to be a viable mode of pain control during endodontic procedures as it converts the solution to a tiny droplet which is then carried by the myelin sheath.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4103/ccd.ccd_466_19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092093PMC
March 2021

Simplified chemical processed CdAlS thin films for high-performance photodetector applications.

J Phys Condens Matter 2021 Apr 26;33(19). Epub 2021 Apr 26.

Department of Chemistry, College of Science, King Abdullah Institute for Nanotechnology, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia.

In this present investigation, we report the effect of aluminum (Al) doping on the photoelectric performance of cadmium sulfide (CdS) thin films prepared by cost-effective automatic nebulizer spray method. The doping of Al concentrations varied from 1 at.% to 9 at.% in the steps of 3 at.%. X-ray diffraction (XRD) patterns show hexagonal crystal structure with polycrystalline nature and the enrichment of crystallite sizes as a function of Al doping concentrations. The formed impurity phase i.e. CdO might be helpful in enhancing the photoelectric performance by its additional photo-generated charge carriers. The optical studies confirm the maximum absorption showed in the visible spectral range with the corresponding minimum bandgap of 2.28 eV for 6 at.% of Al. The room temperature photoluminescence studies show an increase of near-band-edge (NBE) emission as a function of Al doping concentration and this NBE is close to the obtained bandgap in terms of wavelength. In addition, the observed red emission at 635 nm is due to the surface-related impurities or native defect states. From the present work, the observed responsivity (), external quantum efficiency (EQE) and detectivity (*) of the CdS:Al detectors are 8.64 AW, ∼2018% and 9.29 × 10jones, respectively for the optimum 6 at.% of CdS:Al film. The performance of CdS:Al films reported in this work are significantly improved when compared with literature reports. The present investigation, therefore offers a potential material, CdS:Al, as a photodetector for various scientific and industrial applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-648X/abf199DOI Listing
April 2021

Impact of Facultative Bacteria on the Metabolic Function of an Obligate Insect-Bacterial Symbiosis.

mBio 2020 07 14;11(4). Epub 2020 Jul 14.

Department of Entomology, Cornell University, Ithaca, New York, USA

Beneficial microorganisms associated with animals derive their nutritional requirements entirely from the animal host, but the impact of these microorganisms on host metabolism is largely unknown. The focus of this study was the experimentally tractable tripartite symbiosis between the pea aphid , its obligate intracellular bacterial symbiont , and the facultative bacterium which is localized primarily to the aphid hemolymph (blood). Metabolome experiments on, first, multiple aphid genotypes that naturally bear or lack and, second, one aphid genotype from which was experimentally eliminated revealed no significant effects of on aphid metabolite profiles, indicating that does not cause major reconfiguration of host metabolism. However, the titer of just one metabolite, 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), displayed near-significant enrichment in -positive aphids in both metabolome experiments. AICAR is a by-product of biosynthesis of the essential amino acid histidine in and, hence, an index of histidine biosynthetic rates, suggesting that -mediated histidine production is elevated in -bearing aphids. Consistent with this prediction, aphids fed on [C]histidine yielded a significantly elevated C/C ratio of histidine in bearing aphids, indicative of increased (∼25%) histidine synthesized by However, analysis predicted an increase of only 0.8% in histidine synthesis in -bearing aphids. We hypothesize that imposes increased host demand for histidine, possibly for heightened immune-related functions. These results demonstrate that facultative bacteria can alter the dynamics of host metabolic interactions with co-occurring microorganisms, even when the overall metabolic homeostasis of the host is not substantially perturbed. Although microbial colonization of the internal tissues of animals generally causes septicemia and death, various animals are persistently associated with benign or beneficial microorganisms in their blood or internal organs. The metabolic consequences of these persistent associations for the animal host are largely unknown. Our research on the facultative bacterium , localized primarily to the hemolymph of pea aphids, demonstrated that although imposed no major reconfiguration of the aphid metabolome, it did alter the metabolic relations between the aphid and its obligate intracellular symbiont, Specifically, produced more histidine in positive aphids to support both demand for histidine and -induced increase in host demand. This study demonstrates how microorganisms associated with internal tissues of animals can influence specific aspects of metabolic interactions between the animal host and co-occurring microorganisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/mBio.00402-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360925PMC
July 2020

Highly functionalised (γ-azido/γ-fluoro-β-iodo/)vinyl derivatives from phosphorus based allenes or allenoates: IO halogen bonding interactions.

Org Biomol Chem 2019 Jun;17(23):5736-5748

School of Chemistry, University of Hyderabad, Hyderabad 500 046, Telangana, India.

Multifunctional γ-azido/γ-fluoro-β-iodo-vinyl phosphine oxides/phosphonates/esters/sulfone were synthesised by iodination followed by azidation/fluorination of phosphorus-based allenes or allenoates (allenyl esters) or a sulphur based allene. Surprisingly, the reaction of (γ,β)-diiodo-vinyl-phosphonate with TBAF [n-Bu4NF] led to the corresponding allenylphosphonate; in contrast, the use of CsF in a similar reaction led to novel γ-diiodo-allenylphosphonate along with the corresponding non-halogenated allenylphosphonate. The combination AgF2/CuBr could be used to obtain the γ-fluoro-β-iodo-vinyl phosphine oxides and related phosphorus-free γ-fluoro-β-iodo-vinyl esters. In many cases, IO halogen to oxygen non-covalent bonding interactions ('halogen bonding') involving the phosphoryl (P[double bond, length as m-dash]O) oxygen, as evidenced by single crystal X-ray crystallography, are also observed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9ob00715fDOI Listing
June 2019
-->